Tumor immunoreactivity detection kit and use method thereof

Abstract

The invention discloses a tumor immunoreactivity detection kit, and relates to the technical field of detection kits.The tumor immunoreactivity detection kit comprises a kit body, one end of the kit body is rotationally connected with a top cover through a kit body opening and closing assembly, a detection panel is arranged in the kit body, and a reagent detection assembly used for tumor immunoreactivity detection is arranged on the detection panel; an opening and closing plate assembly is arranged at the other end of the box body; a reagent or a sample is sucked through a rubber head dropper in the reagent detection assembly for tumor immunoreactivity detection, the interior and the exterior of the rubber head dropper are synchronously disinfected and cleaned through the dropper disinfecting and cleaning mechanism, a top cover and a box body are sealed through the box body opening and closing assembly, and one end of the box body is sealed through the opening and closing plate assembly. The opening and closing plate is opened so as to transport reagents or samples in the kit body, or the opening and closing plate is opened so as to directly take out and replace the reagent detection area after detection from the outside of the kit body; the device has the advantages of being convenient to use, good in sealing performance, capable of saving resources, good in safety and convenient to transport.

Description

Technical Field

[0001] The present invention relates to the technical field of detection kits, and particularly relates to a tumor immunoreactivity detection kit and a using method thereof. Background Art

[0002] A detection kit is a device used to hold chemical reagents for detecting chemical components, drug residues, virus types, etc. It is generally used in hospitals and pharmaceutical enterprises. At the same time, the kit is also widely used in the detection of tumor immunoreactivity. By detecting a fluid sample with the kit, it is possible to quickly and relatively accurately conduct a preliminary screening on whether a patient is ill. Most existing kits are open box bodies provided with multiple reaction grooves. During the test, the detection test is dropped into the reaction groove, and then the fluid sample is dropped into the reaction groove to be mixed and reacted with the reagent, and the color display is observed for preliminary screening. The disadvantages of existing kits are that the design of the open box body easily causes the fluid sample to splash out, resulting in medical staff or testers coming into contact with the sample, increasing the possibility of infection and having poor safety; at the same time, for the purpose of preventing reagent contamination, products such as droppers in existing products are all for single use, lacking a disinfection and cleaning mechanism for the dropper products, which easily causes the problem of resource waste.

[0003] Existing detection kit products, such as a tumor detection kit provided by CN221419153U with a publication number, include a detection kit body. Installation grooves are symmetrically opened on the inner walls of the detection kit body, and a buffer mechanism is installed inside the installation grooves. A sterile well plate is installed inside the detection kit body, an isolation well plate is installed above the sterile well plate, and a plurality of reagent holes are symmetrically opened above the isolation well plate and extend through the isolation well plate into the sterile well plate. Accommodation cavities are symmetrically opened on both sides of the reagent holes inside the isolation well plate, and a telescopic mechanism is installed inside the accommodation cavities. Adjusting mechanisms are symmetrically installed at the chamfered corners at the bottom end inside the detection kit body; however, this application only focuses on the storage and fixation of detection reagents during transportation and does not design a cleaning and disinfection structure for the droppers used in the detection. Similar to most detection kit products, it easily causes the problem of resource waste. Summary of the Invention

[0004] In view of the above problems, the present invention aims to provide a tumor immunoreactivity detection kit. By designing a box body opening and closing component, through structures such as connecting rods and sliders, when the detection kit is in a closed state, the interior of the box body is sealed. By designing a reagent detection component, a reagent placement area and a reagent detection area are set on the detection panel for reagent detection, and a dropper disinfection and cleaning mechanism is set to disinfect the rubber head dropper. By designing an opening and closing plate component, a movable plate is driven to slide up and down by a slidable button to open or close the side of the box body, facilitating the replacement of the reagent detection area on the detection panel, and having the advantages of convenient use, good sealing performance, resource conservation, good safety, and easy transportation.

[0005] The main idea of the technical solution adopted by the present invention: Design a box body opening and closing component, by setting structures such as connecting rods and sliders between the box body and the top cover to seal the interior of the box body and prevent external bacteria or microorganisms from entering the box body and contaminating the sample or reagent; Design a reagent detection component, by setting a replaceable reagent placement area and a reagent detection area on the detection panel for tumor immunoreactivity detection, and setting a dropper disinfection and cleaning mechanism to disinfect the rubber head dropper to avoid the problem of resource waste caused by frequent replacement of the rubber head dropper; Design an opening and closing plate component, by driving the movable plate to adjust up and down by a button located on one side of the box body, the box body can be sealed or one side of the box body can be opened to facilitate the replacement of the reagent detection area.

[0006] In order to achieve the above object, the technical solution adopted by the present invention is as follows: A tumor immunoreactivity detection kit, comprising a box body, one end of the box body is rotatably connected with a top cover through a box body opening and closing component, a detection panel is arranged in the box body, a reagent detection component for performing tumor immunoreactivity detection is arranged on the detection panel, and an opening and closing plate component is arranged at the other end of the box body.

[0007] The box body opening and closing component includes a first fixed block and a second fixed block fixed on both sides of the top cover and the box body, a double-section connecting rod is rotatably connected between the first fixed block and the second fixed block, a first slider is slidably arranged on the second fixed block, a slider connecting rod is rotatably arranged on the first slider, and the slider connecting rod is rotatably connected with the first fixed block.

[0008] The reagent detection component includes a reagent placement area in the middle of the detection panel, a reagent detection area is slidably installed on one side of the reagent placement area, and a rubber head dropper and a dropper disinfection and cleaning mechanism are arranged on the other side of the reagent placement area.

[0009] Based on the above technical solution, further, the dropper disinfection and cleaning mechanism includes a disinfectant liquid housing fixed to the bottom of the box body. A balloon is provided at the bottom inside the disinfectant liquid housing. An liquid outlet pipe is provided at the top of the disinfectant liquid housing. A double-layer liquid spraying structure is elastically slidably arranged at the top of the liquid outlet pipe. A liquid accumulation tray is fixed to the top of the liquid outlet pipe, and a water-absorbing sponge is also provided on the liquid accumulation tray.

[0010] Based on the above technical solution, further, the double-layer liquid spraying structure includes an inner liquid spraying pipe communicated with the liquid outlet pipe. The middle part of the inner liquid spraying pipe is communicated with an outer liquid spraying pipe through a plurality of liquid infusion ports. A plurality of liquid outlet nozzles are evenly distributed at the tops of the inner liquid spraying pipe and the outer liquid spraying pipe. An inclined plane block matched with the bottom of the rubber head dropper is rotatably arranged between the inner liquid spraying pipe and the outer liquid spraying pipe. A plurality of drainage through holes are formed at the bottom of the inclined plane block. A plurality of total drainage holes staggered with the liquid infusion ports are formed at the bottom of the outer liquid spraying pipe.

[0011] Based on the above technical solution, further, an air pump for continuously inflating the balloon is also provided at the bottom of the box body. A liquid inlet for supplementing the disinfectant liquid is also provided at one side of the top of the disinfectant liquid housing, and a rubber stopper is fixed at the liquid inlet.

[0012] The opening and closing plate assembly includes a sliding plate slidably arranged at one end of the box body. A locking pin is fixed to the top of the sliding plate. A pair of rollers capable of clamping the locking pin are arranged on the box body. A second slider is arranged on one side of each roller. The second slider and both sides of the box body are elastically connected through a slider return spring. A limiting connecting rod is rotatably connected to the second slider, and the end of the limiting connecting rod away from the second slider is rotatably connected to both sides of the box body.

[0013] Based on the above technical solution, further, a sliding button is fixed to the sliding plate, and the sliding button is slidably arranged in a button sliding groove at one end of the box body.

[0014] A using method of a tumor immunoreactivity detection kit includes the following steps: S1. Open the top cover, remove the rubber head dropper located on the detection panel, use the rubber head dropper to suck the reagent from the reagent placement area, and drop it into the reagent detection area for tumor immunoreactivity detection; S2. Press the rubber head dropper downward above the double-layer liquid spraying structure to simultaneously disinfect and clean the inside and outside of the rubber head dropper for the next detection; S3. Push the sliding button upward to drive the sliding plate to slide upward, and clamp and lock the locking pin at the top of the sliding plate through the second slider and the rollers. Then, pull out the reagent detection area from one end of the box body and replace it; S4. Disinfect the rubber head dropper again and put it back on the detection panel. Slide the sliding button downward, and close the top cover through the box body opening and closing assembly to seal the inside of the box body for the overall transportation of the box body.

[0015] The beneficial effects of the present invention are as follows: 1. By designing a reagent detection component, a rubber dropper is used to suck the reagent for tumor immunoreactivity detection, and a dropper disinfection and cleaning mechanism is used to comprehensively disinfect and clean the interior and outer wall of the rubber dropper, enabling the rubber dropper to be repeatedly used for multiple detections, reducing the consumption of rubber droppers, and thus achieving the purpose of saving resources.

[0016] 2. By arranging a box body opening and closing component between the box body and the top cover, through a slider and connecting rod structure, while ensuring that the top cover can be stably opened and closed, a certain fixing effect is provided when the top cover is closed to the top of the box body. Combining with the opening and closing plate component, one end of the box body is sealed by a sliding plate, thereby providing a good sealing effect for the box body, preventing external bacteria or microorganisms from entering the interior of the box body, reducing the possibility of sample contamination, protecting the internal reagent and sample from the influence of the external environment, and improving the overall safety of the device while facilitating the transportation of the entire box body.

[0017] 3. By designing the opening and closing plate component, while providing a sealing effect for the box body, the sliding plate is driven to move upward by a sliding button, so as to replace the reagent detection area inside the box body without disassembling the detection panel, making the present invention also have a certain degree of flexibility.

[0018] 4. By arranging the box body opening and closing component, the reagent detection component and the opening and closing plate component, the use method of the present invention is more convenient, and the overall structure of the present invention is simple and convenient for production. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] Figure 1 is a three-dimensional structural schematic diagram of the present invention; Figure 2 is a top view of the present invention; Figure 3 is a front view of the present invention; Figure 4 is a cross-sectional view of the front view of the present invention; Figure 5 is a front cross-sectional view when the top cover of the present invention is closed; Figure 6 is a three-dimensional structural schematic diagram of the detection panel of the present invention; Figure 7 is a three-dimensional structural schematic diagram of the box body and the opening and closing plate component of the present invention; Figure 8 is Figure 7 a partial detailed view of the opening state of the opening and closing plate component at A; Figure 9 is a partial detailed view of the closed state of the opening and closing plate component; Figure 10Schematic three-dimensional structure diagram of the dropper disinfection and cleaning mechanism; Figure 11 Cross-sectional view of the dropper disinfection and cleaning mechanism; Figure 12 Cross-sectional view of the dropper disinfection and cleaning mechanism at another angle when it is pressed down; Figure 13 For Figure 11 Local detail view at B; Figure 14 For Figure 12 Local detail view at C.

[0020] Wherein: 1. Box body; 101. Detection panel clamping plate; 102. Opening and closing plate sliding groove; 103. Roller sliding groove; 104. Button sliding groove; 105. Object taking groove; 2. Top cover; 3. Box body opening and closing assembly; 301. First fixing block; 302. Second fixing block; 303. Double-section connecting rod; 304. First slider; 305. Slider connecting rod; 306. Fixed pin; 4. Detection panel; 401. Plate body; 402. Detection area installation groove; 403. Rubber head dropper installation groove; 404. Dropper disinfection and cleaning mechanism installation groove; 5. Reagent detection assembly; 501. Reagent placement area; 5011. Detection reagent groove; 5012. Sample groove; 502. Reagent detection area; 5021. Reaction tank; 5022. Color comparison card; 503. Rubber head dropper; 504. Dropper disinfection and cleaning mechanism; 5041. Disinfectant liquid shell; 5042. Airbag; 5043. Liquid outlet pipe; 5044. Double-layer liquid spraying structure; 50441. Inner liquid spraying pipe; 50442. Outer liquid spraying pipe; 50443. Liquid infusion port; 50444. Liquid outlet nozzle; 50445. Inclined block; 50446. Drainage through hole; 50447. Total drainage hole; 5045. Liquid accumulation tray; 5046. Absorbent sponge; 5047. Air pump; 5048. Liquid inlet; 5049. Rubber plug; 5050. Double-layer liquid spraying structure return spring; 6. Opening and closing plate assembly; 601. Sliding plate; 602. Locking pin; 603. Roller; 604. Second slider; 605. Slider return spring; 606. Limit connecting rod; 607. Sliding button. Specific embodiments

[0021] To make the objectives, technical solutions, and advantages of the embodiments of the present application clearer, the technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present application. Obviously, the described embodiments are some, but not all, of the embodiments of the present application. Usually, the components of the embodiments of the present application described and illustrated in the accompanying drawings here can be arranged and designed in various different configurations.

[0022] The inventor's research found that most of the existing reagent kits are open box bodies with multiple reaction grooves. During testing, the detection test is dropped into the reaction groove, and then the fluid sample is dropped into the reaction groove to be mixed and reacted with the reagent, and the color development is observed for preliminary screening. The disadvantages of the existing reagent kits are that the design of the open box body easily causes the fluid sample to splash out, resulting in medical staff or testers coming into contact with the sample, increasing the possibility of infection and having poor safety. At the same time, for the purpose of preventing reagent contamination, products such as droppers in existing products are all for single use, lacking a disinfection and cleaning mechanism for dropper products, which easily causes waste of resources.

[0023] Based on the above findings, the present application proposes a tumor immunoreactivity detection reagent kit, a box body opening and closing assembly, by setting structures such as connecting rods and sliders between the box body and the top cover to seal the interior of the box body and prevent external bacteria or microorganisms from entering the box body and contaminating the sample or reagent; designing a reagent detection assembly, by setting replaceable reagent placement areas and reagent detection areas on the detection panel for tumor immunoreactivity detection, and setting a dropper disinfection and cleaning mechanism to disinfect the rubber dropper, avoiding the problem of resource waste caused by frequent replacement of the rubber dropper; designing an opening and closing plate assembly, which can seal the box body or open one side of the box body by driving the movable plate to move up and down with a button located on one side of the box body, so as to replace the reagent detection area; having the advantages of convenient use, good sealing performance, resource saving, good safety and easy transportation.

[0024] Refer to Figures 1 - 5 As shown, the present application discloses a tumor immunoreactivity detection reagent kit, including a box body 1. One end of the box body 1 is rotatably connected to a top cover 2 through a box body opening and closing assembly 3. When the box body 1 and the top cover 2 are combined, they can exactly form a complete rectangular parallelepiped shell structure. A horizontal object taking groove 105 is opened at one end of the box body 1. The overall material of the box body 1 and the top cover 2 should be selected from materials with good strength and corrosion resistance.

[0025] The box body opening and closing assembly 3 includes a first fixed block 301 and a second fixed block 302 fixed to both sides of the top cover 2 and the box body 1. In some embodiments, the cross-sectional shape of the first fixed block 301 is approximately a right trapezoid, and it is symmetrically fixed to both sides of the top cover 2 by means of bolt connection or bonding. The second fixed block 302 is a flat rectangular parallelepiped structure with a slide rail opened in the middle, and two second fixed blocks 302 are symmetrically installed on both sides of the box body 1 by screwing fixing pins 306 into both ends thereof; A double-segment connecting rod 303 is rotatably connected between the first fixed block 301 and the second fixed block 302. A first slider 301 is slidably arranged on the slide rail in the middle of the second fixed block 302. A slider connecting rod 305 is rotatably arranged on the first slider 301, and the slider connecting rod 305 is rotatably connected to the first fixed block 301. Then, when the top cover 2 is opened or closed, through the rotational action of the double-segment connecting rod 303 and the first slider 301 and the slider connecting rod 305, the opening and closing of the top cover 2 can be made smoother. When the top cover is in the fully open state, through the slider and connecting rod structure of the box body opening and closing assembly 3, the top cover 2 can be prevented from falling off. When the top cover 2 is in the closed state, through the first slider 304 and the slider connecting rod 305, a certain degree of limiting effect can be exerted on the top cover 2 to prevent the top cover 2 from being opened during transportation, causing the internal tumor immunoreactivity detection reagent to spill to the outside and causing environmental pollution, or preventing bacteria or microorganisms from the outside from entering the inside of the box body, reducing the possibility of sample contamination, protecting the internal reagent and sample from being affected by the external environment, and being beneficial to improving the overall safety of the device.

[0026] At the same time, since some reagents or samples are sensitive to environmental temperature and humidity, by setting the box body opening and closing assembly 3 to keep the inside of the box body 1 sealed, the temperature and humidity inside the box body 1 can also be kept in a relatively stable state. In some embodiments, a temperature control board or other structures can be selected to be arranged inside the box body 1 to adjust the temperature and humidity inside the box body 1, so as to keep the reagent or sample in good activity, and further ensure the accuracy and stability of the detection results.

[0027] In some embodiments, a driving mechanism can also be selected to be arranged at each rotational connection of the box body opening and closing assembly 3, and a control button capable of actuating the driving mechanism is arranged on the box body 1, so that the box body 1 can be opened or kept in the closed state through the control button, so as to improve the overall usability of the device.

[0028] A detection panel 4 is arranged in the box body 1. Specifically, the detection panel 4 includes a plate body 401. A detection area installation groove 402 is opened at one end of the plate body 401, and a rubber head dropper installation groove 403 and a dropper disinfection and cleaning mechanism installation groove 404 are opened at the other end of the plate body 401. Two detection panel clamping plates 101 are fixed on both sides inside the box body 1 and below the second fixed block 302. The overall area of the detection panel 4 matches the inside of the box body 1, and the plate body 401 of the detection panel 4 can be placed into the box body 1 from the top of the box body 1 or from the access slot 105 at one end of the box body 1.

[0029] Refer to Figure 6, a reagent detection assembly 5 for tumor immunoreactivity detection is provided on the detection panel 4. The reagent detection assembly 5 includes a reagent placement area 501 located in the middle of the detection panel 4, that is, in the middle of the plate body 401. The reagent placement area 501 includes a fixing frame structure that can be fixed to the middle of the plate body 401. A plurality of detection reagent grooves 5011 and sample grooves 5012 are fixed on both sides of the fixing frame structure. The structures of the detection reagent grooves 5011 and the sample grooves 5012 are similar to test tubes, which can prevent reagents or samples from spilling out when the box body 1 is in the transportation state. By classifying and placing the reagents and samples for tumor immunoreactivity detection, it is convenient for operators to perform detections; On one side of the reagent placement area 501, that is, in the detection area installation groove 402, a reagent detection area 502 is slidably installed. In some embodiments, the reagent detection area 502 can be regarded as a multi-channel reagent detection plate. Reaction grooves 5021 and color comparison cards 5022 are provided on the detection plate. By using a rubber dropper 503 to respectively suck the reagents and samples and put them into the reaction grooves 5021, and the reaction results are detected by comparing with the color comparison card. After the detection is completed, the reagent detection area 502 can be pulled out from the detection panel 4 through the extraction slot 105 at one end of the box body 1, and a new reagent detection area 502 can be replaced.

[0030] A rubber dropper 503 is provided in the rubber dropper installation groove 403 on the other side of the reagent placement area 501, and a dropper disinfection and cleaning mechanism 504 is provided in the dropper disinfection and cleaning mechanism installation groove 404. The rubber dropper 503 is similar to existing rubber dropper products. In some embodiments, the bottom of the rubber dropper 503 is of an oblique cutting structure and can cooperate with the inclined plane block 50445 in the dropper disinfection and cleaning mechanism 504.

[0031] Refer to Figures 10 - 14 , the dropper disinfection and cleaning mechanism 504 includes a disinfectant liquid shell 5041 fixed to the bottom of the box body 1. In some embodiments, the disinfectant liquid shell 5041 is of a cylindrical shell structure, and its interior is filled with disinfectant liquids such as alcohol; At the bottom inside the disinfectant solution housing 5041, there is an airbag 5042. At the top of the disinfectant solution housing 5041, there is a liquid outlet pipe 5043. At the top of the liquid outlet pipe 5043, a double-layer liquid spraying structure 5044 is elastically slidably arranged. When the bottom of the dropper 503 contacts the top of the double-layer liquid spraying structure 5044 and presses it down, the disinfectant solution in the disinfectant solution housing 5041 can be squeezed, and the disinfectant solution can be backflowed upward through the liquid outlet pipe 5043 into the double-layer liquid spraying structure 5044, and sprayed onto the inside and outer wall of the dropper 503 to disinfect and clean the dropper 503. Before each time of sucking a reagent or a sample, the dropper 503 is cleaned and disinfected through the double-layer liquid spraying structure 5044, so that the dropper 503 can be used for multiple detections repeatedly, thereby reducing the usage amount of the dropper 503, and further achieving the purpose of saving resources; At the top of the liquid outlet pipe 5043, a liquid collecting tray 5045 is fixed. On the liquid collecting tray 5045, there is also a water-absorbing sponge 5046. The liquid collecting tray 5045 is a disc-shaped structure with the bottom lower than the edge, which can gather the disinfectant solution discharged after disinfection towards the center at its bottom. At the same time, the water-absorbing sponge 5046 is an annular structure, arranged along the middle of the liquid collecting tray 5045, and the inner diameter of the cross-section of the water-absorbing sponge 5046 matches the outer diameter of the cross-section of the double-layer liquid spraying structure 5044. When the double-layer liquid spraying structure 5044 is pressed down by the dropper 503, the water-absorbing sponge 5046 will not obstruct the double-layer liquid spraying structure 5044. The disinfectant solution after disinfecting the dropper 503 can be discharged from the outer wall of the double-layer liquid spraying structure 5044 and absorbed through the water-absorbing sponge 5046, which can effectively recycle the part of the disinfectant solution sprayed to the outside and prevent it from leaking to the external environment and causing environmental pollution.

[0032] The double-layer liquid spraying structure 5044 includes an inner liquid spraying pipe 50441 communicated with the liquid outlet pipe 5043. The inner liquid spraying pipe 50441 is a hollow cylindrical tubular structure provided with three boss structures. The top of the boss at its bottommost can be clamped by the bottom of the boss near the top of the liquid outlet pipe 5043, and there are also a plurality of liquid inlet ports near the boss at the bottom of the inner liquid spraying pipe 50441. When the double-layer liquid spraying structure 5044 is pressed down, the bottom boss of the inner liquid spraying pipe 50441 is disengaged from the clamping with the liquid outlet pipe 5043, so that the disinfectant solution in the liquid outlet pipe 5043 flows into the inner liquid spraying pipe 50441 through the liquid inlet ports; Meanwhile, a double-layer liquid spraying structure return spring 5050 is provided between the top of the top boss of the liquid outlet pipe 5043 and the bottom of the middle boss of the inner liquid spraying pipe 50441. When the rubber head dropper 503 no longer presses down the double-layer liquid spraying structure 5044, the inner liquid spraying pipe 50441 is lifted upward along the liquid outlet pipe 5043 as a whole through the double-layer liquid spraying structure return spring 5050, so that the bottom boss of the inner liquid spraying pipe 50441 is clamped with the liquid outlet pipe 5043 again, completing the overall reset of the double-layer liquid spraying structure 5044. At this time, the disinfectant in the liquid outlet pipe 5043 no longer flows into the inner liquid spraying pipe 50441; The middle part of the inner liquid spraying pipe 50441 is communicated with the outer liquid spraying pipe 50442 through a plurality of liquid infusion ports 50443. At the same time, the bottom of the outer liquid spraying pipe 50442 is fixed to the topmost boss of the inner liquid spraying pipe 50441. A plurality of liquid outlet nozzles 50444 are evenly distributed at the tops of the inner liquid spraying pipe 50441 and the outer liquid spraying pipe 50442. When the double-layer liquid spraying structure 5044 is pressed down as a whole, the disinfectant flowing into the inner liquid spraying pipe 50441 flows into the outer liquid spraying pipe 50442 through the liquid infusion ports 50443 and is sprayed out through the liquid outlet nozzles 50444. At this time, since the rubber head dropper 503 is integrally sleeved between the inner liquid spraying pipe 50441 and the outer liquid spraying pipe 50442, the disinfectant can simultaneously disinfect and clean the inside and outside of the rubber head dropper 503; In some embodiments, the inner diameter of the liquid outlet nozzle 50444 is smaller than the inner diameter of the liquid infusion port 50443. Then, when the double-layer liquid spraying structure 5044 is pressed down, the disinfectant flowing into the inner liquid spraying pipe 50441 will preferentially flow into the outer liquid spraying pipe 50442 through the liquid infusion ports 50443 and then be sprayed out through the liquid outlet nozzles 50444; A bevel block 50445 matched with the bottom of the rubber head dropper 503 is rotatably arranged between the inner liquid spraying pipe 50441 and the outer liquid spraying pipe 50442. When the bottom of the rubber head dropper 503 is inserted between the inner liquid spraying pipe 50441 and the outer liquid spraying pipe 50442, it can contact the bevel block 50445 and make it rotate, and finally fit with the bottom of the rubber head dropper 503, so as to stably press down the double-layer liquid spraying structure 5044 as a whole; A plurality of drainage holes 50446 are provided at the bottom of the inclined surface block 50445, and a plurality of main drainage holes 50447 interlaced with the infusion port 50443 are provided at the bottom of the external liquid spraying tube 50442. After disinfection, the disinfectant flows downward along the gap between the inclined surface block 50445 and the internal liquid spraying tube 50441, and the gap between the inclined surface block 50445 and the external liquid spraying tube 50442, respectively. The disinfectant between the inclined surface block 50445 and the internal liquid spraying tube 50441 can be merged into the space between the inclined surface block 50445 and the external liquid spraying tube 50442 through the drainage holes 50446 at the bottom of the inclined surface block 50445, and discharged to the absorbent sponge 5046 located on the liquid accumulation tray 5045 through the main drainage holes 50447, so as to recover and absorb the disinfectant.

[0033] The bottom of the box body 1 is also provided with an air pump 5047 for continuously inflating the airbag 5042. The top side of the disinfectant housing 5041 is also provided with a liquid inlet 5048 for replenishing disinfectant. A rubber plug 5049 is fixed to the liquid inlet 5048. Disinfectant is replenished into the disinfectant housing 5041 through the liquid inlet 5048. At the same time, the rubber plug 5049 can provide a sealing effect for the disinfectant housing 5041 and will not be opened by the liquid pressure in the disinfectant housing 5041. The air pump 5047 is a low-power air pump product, which can provide continuous but trace amounts of gas to the airbag 5042. When there is a large amount of residual disinfectant in the disinfectant housing 5041, the disinfectant can be squeezed upward, thereby further ensuring the clamping state of the bottom boss of the inner spray tube 50441 and the liquid outlet tube 5043, preventing the disinfectant from flowing into the inner spray tube 50441. After the rubber-tipped dropper 503 performs multiple disinfections, the residual disinfectant in the disinfectant housing 5041 is reduced. The expansion effect of the airbag 5042 can make up for the reduced amount of disinfectant, so that the disinfectant is maintained in a state that can be triggered by the double-layer spray structure 5044, thereby improving the overall durability of the device.

[0034] The other end of the box body 1, i.e., the end of the box body 1 on which the taking slot 105 is provided, is provided with an opening and closing plate assembly 6, and the opening and closing plate assembly 6 includes a vertically arranged sliding plate 601, and both sides of the end of the box body 1 on which the taking slot 105 is provided are provided with opening and closing plate sliding grooves 102 that match the two ends of the sliding plate 601, and when the sliding plate 601 slides to the bottom along the opening and closing plate sliding grooves 102, it can just block the taking slot 105 provided on the box body 1; A locking pin 602 is fixed on the top of the sliding plate 601. The locking pin 602 is a hollow pin-shaped structure with a certain elasticity. The overall width of the middle part is greater than the top and the bottom. The bottom of the locking pin 602 is an inwardly concave semicircular shape, and the top is a spike-shaped structure. On the box body 1, and above the sliding plate 601, there is a pair of rollers 603 capable of clamping the bottom of the locking pin 602. Each roller 603 is provided with a shaft member in the middle, and is respectively slidably installed in two roller chutes 103 opened on the box body 1 through the shaft member. Each roller 603, and on the side away from between the two rollers 603, is provided with a second slider 604. The second slider 604 is elastically connected to both sides of the box body 1 through a slider return spring 605. Under the action of the slider return spring 605, the second slider 604 can push the roller 603 towards the middle position under normal conditions. Since the roller 603 is clamped in the roller chute 103, it will not fall from the hole through which the locking pin 602 passes. When the sliding plate 601 slides upward, the roller 603 can be pushed towards both ends by the tip at the top of the locking pin 602. When the sliding plate 601 continues to slide upward until the bottom of the locking pin 602 contacts the rollers 603 on both sides, under the action of the second slider 604 and the slider return spring 605, the roller 603 can clamp the bottom of the locking pin 602, thereby fixing the sliding plate 601. At this time, the sliding plate 601 no longer blocks the access slot 105 at one end of the box body 1, facilitating the operator to directly take out the reagent detection area 502 or the entire detection panel 4 from the box body 1; A limiting connecting rod 606 is rotatably connected to the second slider 604. The end of the limiting connecting rod 606 away from the second slider 604 is rotatably connected to both sides of the box body 1 near the top. By setting the limiting connecting rod 606, the second slider 604 can be made to fit with the bottom at its position during sliding, preventing the second slider 604 from moving upward under the squeezing action of the slider return spring 605 and the roller 603, thereby improving the overall reliability of the device. In some implementation schemes, it is also possible to choose to open chutes on both sides of the bottom at its position, and provide a limiting plate structure on the bottom of the second slider 604 that matches the chute to achieve the purpose of preventing the second slider 604 from moving upward.

[0035] A sliding button 607 is fixed on the sliding plate 601. The sliding button 607 is slidably arranged in a button chute 104 at one end of the box body 1. The button chute 104 is vertically opened along the side of the box body 1 where the access slot 105 is opened, and the button chute 104 is located above the access slot 105. By pushing the sliding button 607 upward or downward, and driving the sliding plate 601 to open or block the access slot 105 on the box body 1, it is convenient for the operator to replace the reagent detection area 502, or to provide a good sealing effect for the box body 1, thereby improving the overall flexibility of the device.

[0036] A method for using an oral opening device applicable to clinical dentistry includes the following steps: S1, open the top cover 2, remove the rubber-tipped dropper 503 on the detection panel 4, use the rubber-tipped dropper 503 to draw reagent from the reagent placement area 501, and drop it into the reagent detection area 502 to perform tumor immune reactivity detection; S2, press the rubber-tipped dropper 503 downward along the double-layer liquid spray structure 5044 to simultaneously disinfect and clean the inside and outside of the rubber-tipped dropper 503 for the next test; S3, push the sliding button 607 upward to drive the sliding plate 601 to slide upward, and lock the locking pin 602 on the top of the sliding plate 601 by the second slider 604 and the roller 603, and then pull out the reagent detection area 502 from one end of the box body 1 and replace it; S4. After disinfecting the rubber-tipped dropper 503 again, put it back on the detection panel 4, slide the sliding button 607 downward, and close the top cover 2 through the box body opening and closing component 3 to seal the inside of the box body 1 so that the box body 1 can be transported as a whole.

[0037] Practical application cases By lifting the top cover 2, the device is opened as a whole, and the reagents and samples required for tumor immune reactivity detection are added to the reagent placement area 501 on the detection panel 4 for detection and use. If the sample is a solid tissue, the sample can be pre-processed by shredding, digestion, and preparation of single cell suspension, and then directly placed in the sample slot 5012; when the reagents and samples in the box body 1 need to be transported, the top cover 2 and the sliding plate 601 are closed to seal the inside of the box body 1 for transportation; When tumor immune reactivity detection is required, the top cover 2 is opened, and the rubber-tipped dropper 503 is taken out. First, the rubber-tipped dropper 503 is inserted between the inner liquid spraying tube 50441 and the outer liquid spraying tube 50442, and the rubber-tipped dropper 503 is pressed down. The disinfectant in the disinfectant shell 5041 is sucked by the double-layer liquid spraying structure 5044 to disinfect the rubber-tipped dropper 503. Then, the reagent or sample is sucked by the rubber-tipped dropper 503 and placed in the reaction tank 5021 of the reagent detection area 502 for tumor immune response, and the detection result is compared with the colorimetric card 5022. The rubber-tipped dropper 503 needs to be disinfected and cleaned in the interval between each reagent or sample suction and after the detection. When the tumor immune reactivity test is completed, push the sliding button 607 to move the sliding plate 601 upward, so that the reagent detection area 502 can be removed from the sample retrieval slot 105 without removing the detection panel 4, and replaced with a new reagent detection area 502 for the next tumor immune reactivity test.

[0038] The foregoing has shown and described the basic principles, main features and advantages of the present invention. Those skilled in the art should understand that the present invention is not limited by the above embodiments, and what is described in the above embodiments and the specification is only to illustrate the principles of the present invention. Without departing from the spirit and scope of the present invention, the present invention will have various changes and improvements, and these changes and improvements fall within the scope of the present invention claimed. The scope of protection claimed by the present invention is defined by the appended claims and their equivalents.

Claims

1. A tumor immune reactivity detection kit, comprising a kit body (1), characterized in that: The box body (1) is rotatably connected to the top cover (2) via a box body opening and closing assembly (3); a detection panel (4) is provided in the box body (1); a reagent detection assembly (5) for detecting tumor immune reactivity is provided on the detection panel (4); and an opening and closing plate assembly (6) is provided at the other end of the box body (1).

2. A tumor immune reactivity detection kit according to claim 1, characterized in that: The box body opening and closing assembly (3) comprises a first fixed block (301) and a second fixed block (302) fixed to the top cover (2) and both sides of the box body (1); a two-section connecting rod (303) is rotatably connected between the first fixed block (301) and the second fixed block (302); a first sliding block (304) is slidably arranged on the second fixed block (302); and the first sliding block (304) is rotatably connected to the first fixed block (301) via a sliding block connecting rod (305).

3. A tumor immune reactivity detection kit according to claim 1, characterized in that: The reagent detection assembly (5) comprises a reagent placement area (501) located on the detection panel (4), a reagent detection area (502) is provided on one side of the reagent placement area (501), and a rubber-tipped dropper (503) and a dropper disinfection and cleaning mechanism (504) are provided on the other side of the reagent placement area (501).

4. A tumor immune reactivity detection kit according to claim 3, characterized in that: The dropper disinfecting and cleaning mechanism (504) comprises a disinfectant shell (5041) fixed to the bottom of the box body (1), an air bag (5042) is arranged inside the disinfectant shell (5041), a liquid outlet pipe (5043) is arranged at the top of the disinfectant shell (5041), a double-layer liquid spraying structure (5044) is elastically slidably arranged at the top of the liquid outlet pipe (5043), a liquid collection tray (5045) is fixed at the top of the liquid outlet pipe (5043), and a water-absorbing sponge (5046) is also arranged on the liquid collection tray (5045).

5. A tumor immune reactivity detection kit according to claim 4, characterized in that: The double-layer liquid spray structure (5044) comprises an inner liquid spray tube (50441) connected to a liquid outlet tube (5043); the middle of the inner liquid spray tube (50441) is connected to an outer liquid spray tube (50442) via a plurality of liquid infusion ports (50443); a plurality of liquid outlet nozzles (50444) are evenly distributed on the tops of the inner liquid spray tube (50441) and the outer liquid spray tube (50442); an inclined surface block (50445) that matches the bottom of the rubber-tipped dropper (503) is rotatably arranged between the inner liquid spray tube (50441) and the outer liquid spray tube (50442); a plurality of drainage through holes (50446) are provided at the bottom of the inclined surface block (50445); and a plurality of total drainage holes (50447) that are interlaced with the liquid infusion ports (50443) are provided at the bottom of the outer liquid spray tube (50442).

6. A tumor immune reactivity detection kit according to claim 4, characterized in that: An air pump (5047) for continuously inflating the airbag (5042) is also provided at the bottom of the box body (1), and a liquid inlet (5048) for replenishing the disinfectant is also provided on one side of the top of the disinfectant housing (5041), and a rubber plug (5049) is fixed to the liquid inlet (5048).

7. A tumor immune reactivity detection kit according to claim 1, characterized in that: The opening and closing plate assembly (6) comprises a sliding plate (601) slidably arranged at one end of the box body (1), a locking pin (602) being fixed to the top of the sliding plate (601), a pair of rollers (603) capable of engaging the locking pin (602) being arranged on the box body (1), a second sliding block (604) elastically connected to two sides of the box body (1) being arranged on one side of each roller (603), and the second sliding block (604) being rotatably connected to two sides of the box body (1) via a limiting connecting rod (606).

8. A tumor immune reactivity detection kit according to claim 7, characterized in that: A sliding button (607) is fixed on the sliding plate (601), and the sliding button (607) is slidably arranged in a button sliding groove (104) at one end of the box body (1).

9. A method for using a tumor immune reactivity detection kit, characterized in that: The following steps are involved: S1, open the top cover (2), remove the rubber-tipped dropper (503) located on the detection panel (4), use the rubber-tipped dropper (503) to draw the reagent from the reagent placement area (501), and drop it into the reagent detection area (502) to perform tumor immune reactivity detection; S2, pressing the rubber-tipped dropper (503) downward along the top of the double-layer liquid spraying structure (5044) to simultaneously disinfect and clean the inside and outside of the rubber-tipped dropper (503) for the next test; S3, push the sliding button (607) upward to drive the sliding plate (601) to slide upward, and lock the locking pin (602) on the top of the sliding plate (601) by means of the second sliding block (604) and the roller (603), and then extract the reagent detection area (502) from one end of the box body (1) and replace it; S4. After the rubber-tipped dropper (503) is disinfected again, it is placed back on the detection panel (4), the sliding button (607) is slid downward, and the top cover (2) is closed by the box body opening and closing assembly (3) so that the inside of the box body (1) is sealed so that the box body (1) can be transported as a whole.

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