Cell culture meat food hazard factor screening system and equipment

Abstract

The invention belongs to the technical field of cell culture meat screening, and particularly relates to a cell culture meat food hazard factor screening system and device.The cell culture meat food hazard factor screening system comprises a sensing unit, an execution unit, an analysis unit and a control unit; the sensing unit comprises an integrated sensor and living cell imaging equipment, the integrated sensor is used for collecting the culture environment of cell culture meat in real time, and the living cell imaging equipment is used for dynamically tracking the cell growth state; the execution unit is used for executing sample data acquisition and detection according to presetting; and the analysis unit analyzes and processes the data information sent by the sensing unit and the execution unit based on an AI data analysis platform. The intelligent level of screening the harmful factors of the cell culture meat can be greatly improved, so that dynamic evaluation and early warning of the harmful factors in the production process of the cell culture meat are realized, workers can timely and effectively perform corresponding treatment, and the production quality of the cell culture meat is further ensured.

Description

Technical Field

[0001] The present invention belongs to the technical field of cell - cultured meat screening, and particularly relates to a cell - cultured meat food hazard factor screening system and device. Background Art

[0002] Cell - cultured meat food refers to a kind of food that uses cell culture engineering and tissue engineering technologies to cultivate animal muscle tissue in vitro as edible material. Specifically, it is achieved by collecting and storing animal cells, and then culturing them in a bioreactor. The cells gradually multiply in a culture medium rich in oxygen, nutrients, and growth factors. Sometimes, an edible scaffold is also used to allow the cells to multiply around it, thus forming a structured tissue. By changing the composition of the culture medium, these cells can differentiate into muscle, fat, or connective tissue. Compared with traditional animal meat, cell - cultured meat shows more scientific controllability, with clear production purposes, high production efficiency, low engineering metabolism in the production process, and advantages such as rich edible protein types and food markets.

[0003] During the production process of cell - cultured meat, hazard factors such as allergens, food - borne pathogenic bacteria, and new toxins may be introduced or exist. In order to timely and effectively remove hazard factors and ensure the production quality of cell - cultured meat, it is necessary to screen cell - cultured meat hazard factors. However, the existing intelligent level of cell - cultured meat hazard factor screening is low, and it cannot achieve dynamic assessment and early warning of hazard factors, resulting in the need for staff to perform periodic sampling and detection. Moreover, the instantaneous fluctuations of environmental parameters will affect the production of cell - cultured meat. Periodic sampling and detection have lag, and at the same time, it will also increase the burden on staff.

[0004] Therefore, in view of the above - mentioned technical problems, it is necessary to provide a cell - cultured meat food hazard factor screening system and device.

[0005] The information disclosed in this background art section is only intended to enhance the overall understanding of the present invention and should not be regarded as an admission or any form of implication that this information constitutes prior art already known to those of ordinary skill in the art. Summary of the Invention

[0006] The purpose of the present invention is to provide a cell - cultured meat food hazard factor screening system and device, which can solve the problem of low intelligent level of cell - cultured meat hazard factor screening.

[0007] To achieve the above - mentioned purpose, the technical solution provided by a specific embodiment of the present invention is as follows:

[0008] A cell - cultured meat food hazard factor screening system includes: a sensing unit, an execution unit, an analysis unit, and a control unit;

[0009] The sensing unit includes an integrated sensor and a live cell imaging device. The integrated sensor is used to collect the parameters of the cell-cultured meat culture environment in real time, and the live cell imaging device is used to dynamically track the cell growth state;

[0010] The execution unit is used to collect and detect sample data according to a preset execution;

[0011] The analysis unit analyzes and processes the data information sent by the sensing unit and the execution unit based on an AI data analysis platform;

[0012] The control unit dynamically adjusts the execution unit according to the analysis result of the analysis unit and triggers a traceability warning;

[0013] Among them, the execution unit includes a primary screening module and a re-inspection module. The primary screening module is used to quickly detect the microbial and chemical indicators of cell-cultured meat, and the re-inspection module is used to conduct in-depth laboratory analysis on the samples that fail the primary screening.

[0014] In one or more embodiments of the present invention, the integrated sensor is used to monitor the pH value, dissolved oxygen content, and temperature of the cell-cultured meat culture environment in real time, so as to realize the dynamic assessment and warning of hazard factors;

[0015] The live cell imaging device is a CellAssist 50 system. The CellAssist 50 system uses label-free high-throughput imaging, can monitor 50 microplates at the same time, perform more than 100-layer scans, and the imaging depth can reach 4 mm in the Z-axis imaging; the CellAssist 50 system supports two high-resolution imaging methods: bright field and phase contrast, and is equipped with 4x, 10x, and 20x lenses, with fast imaging speed; it has a unique Z-axis imaging function: CellAssist 50 can perform label-free 3D imaging, capture clear images of more than 100 focal planes, and the distance between each focal plane is 2 μm to 50 μm; it can be compatible with a variety of samples; it has powerful analysis capabilities: CellAssist 50 can perform big data fast analysis, output indicators such as cell fusion rate curve, number of cell clusters, doubling time, IC50, etc., and it also has a clone tracking function, which can trace the origin of each clone cluster and manage the data of multiple plates at the same time;

[0016] The live cell imaging device in this application can automatically scan the cell morphology changes every 15 minutes to warn of early apoptosis or abnormal proliferation.

[0017] In one or more embodiments of the present invention, the device of the primary screening module is a rapid detector for diseased meat. The rapid detector for diseased meat adopts a four-wavelength cold light source and a rotating colorimetric cell design, and has the characteristics of high efficiency, accuracy, real-time monitoring, simple operation, and versatility, which is convenient for quickly detecting cell-cultured meat;

[0018] The re-inspection module performs in-depth analysis on the initially screened unqualified samples based on whole-genome sequencing technology, which can comprehensively screen for unknown pathogenic microorganisms and trace the pollution pathway.

[0019] In one or more embodiments of the present invention, the AI data analysis platform constructs a detection database by combining machine learning algorithms and Internet of Things technology, and based on the historical data in the database, realizes the dynamic assessment of risk factors and forms a risk heat map.

[0020] In one or more embodiments of the present invention, the control unit includes a traceability platform. The control unit optimizes the detection cycle of the execution unit in combination with the dynamic assessment results and feeds back the unqualified results of the samples to the traceability platform, and uses the traceability platform to locate the pollution source.

[0021] A screening device for harmful factors in cell-cultured meat products includes a rapid detector for diseased meat, which is applied to a screening system for harmful factors in cell-cultured meat products. The rapid detector for diseased meat includes: a main body of the diseased meat detector, a fixing frame, and a plurality of re-inspection sample temporary storage mechanisms;

[0022] A pair of connecting rods are fixedly connected to the fixing frame, and the fixing frame is installed on the side wall of the main body of the diseased meat detector through the connecting rods. A plurality of installation grooves are provided on the fixing frame;

[0023] A plurality of the re-inspection sample temporary storage mechanisms are respectively arranged in the plurality of installation grooves. The re-inspection sample temporary storage mechanism includes a temporary storage tank. A storage tube and a heat preservation liquid are arranged in the temporary storage tank. The storage tube is arranged in the heat preservation liquid, and an end cover is threadedly connected to the temporary storage tank.

[0024] In one or more embodiments of the present invention, a fixed outer ring is connected to the outer wall of the temporary storage tank. The diameter of the fixed outer ring is larger than the diameter of the installation groove, and the fixed outer ring contacts the upper end surface of the fixing frame. When the temporary storage tank is placed in the installation groove, the fixed outer ring contacts the upper end surface of the fixing frame to support the temporary storage tank;

[0025] A fixed inner ring is provided on the inner wall of the temporary storage tank. A plurality of fixing columns are connected between the fixed inner ring and the inner wall of the temporary storage tank. The inner diameter of the fixed inner ring is larger than the outer diameter of the storage tube. The fixed inner ring is used to stabilize the storage tube, prevent the storage tube from moving in the temporary storage tank, and ensure the installation of the initially screened unqualified samples in the storage tube.

[0026] In one or more embodiments of the present invention, a top cover is connected to the inner top wall of the end cover, and the top cover contacts the top wall of the storage tube to further fix the storage tube;

[0027] A cavity is provided inside the end cap, and a plurality of self - melting cooling particles are provided inside the cavity. The self - melting cooling particles are used to lower the temperature of the heat - insulating liquid, avoiding problems such as deterioration of the initially screened unqualified samples in the storage tube during the re - inspection transportation, and thus ensuring the re - inspection effect of the initially screened unqualified samples in the follow - up.

[0028] A release tube is connected to the end cap, and the cavity is connected to the inside of the temporary storage tank through the release tube. The self - melting cooling particles in the cavity can enter the temporary storage tank through the release tube and enter the heat - insulating liquid.

[0029] A control valve is installed on the release tube to control the on - off of the release tube.

[0030] In one or more embodiments of the present invention, a panel is installed on the outer top wall of the end cap, and the control valve is electrically connected to the panel. The opening state of the control valve can be controlled by using the panel.

[0031] A supplementary cover door is provided on the side wall of the end cap, and the supplementary cover door corresponds to the cavity, facilitating the replenishment of self - melting cooling particles into the cavity.

[0032] In one or more embodiments of the present invention, a temperature sensor is installed on the inner wall of the temporary storage tank. The temperature sensor is located below the fixed inner ring, and the temperature sensor is electrically connected to the panel. The temperature sensor is used to monitor the temperature of the heat - insulating liquid, so as to timely add self - melting cooling particles into the heat - insulating liquid, use the self - melting cooling particles to lower the temperature of the heat - insulating liquid, avoid problems such as deterioration of the initially screened unqualified samples in the storage tube during the re - inspection transportation, and thus ensure the re - inspection effect of the initially screened unqualified samples in the follow - up.

[0033] Compared with the prior art, a cell - cultured meat food hazard factor screening system and equipment of the present invention can greatly improve the intelligent level of cell - cultured meat hazard factor screening, realize the dynamic assessment and early warning of hazard factors during the production process of cell - cultured meat, so that the staff can make corresponding treatments in a timely and effective manner, and thus ensure the production quality of cell - cultured meat. BRIEF DESCRIPTION OF THE DRAWINGS

[0034] In order to more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the following will briefly introduce the drawings required for the description of the embodiments or the prior art. Obviously, the drawings described below are only some embodiments recorded in the present invention. For those of ordinary skill in the art, other drawings can be obtained based on these drawings without creative efforts.

[0035] Figure 1 It is a framework diagram of a cell - cultured meat food hazard factor screening system in an embodiment of the present invention.

[0036] Figure 2 Stereogram of a screening device for harmful factors in cell-cultured meat products in an embodiment of the present invention;

[0037] Figure 3 Sectional view of the retest sample temporary storage mechanism in an embodiment of the present invention;

[0038] Figure 4 For Figure 3 Schematic diagram of the structure at position A in

[0039] Figure 5 For Figure 3 Schematic diagram of the structure at position B in

[0040] Figure 6 First-angle stereogram of the retest sample temporary storage mechanism in an embodiment of the present invention;

[0041] Figure 7 Second-angle stereogram of the retest sample temporary storage mechanism in an embodiment of the present invention;

[0042] Figure 8 Sectional view of the self-fusing cooling particles in an embodiment of the present invention;

[0043] Figure 9 For Figure 8 Schematic diagram of the structure at position C in

[0044] Figure 10 Schematic diagram of the principle of the auxiliary decision-making system of a screening device for harmful factors in cell-cultured meat products in an embodiment of the present invention.

[0045] Main reference numeral description:

[0046] 1 - Main body of the diseased meat detector, 2 - Fixed frame, 201 - Connecting rod, 3 - Retest sample temporary storage mechanism, 301 - Temporary storage tank, 3011 - Fixed outer ring, 3012 - Fixed inner ring, 302 - Storage tube, 303 - Heat preservation liquid, 304 - End cover, 3041 - Top cover, 3042 - Release tube, 3043 - Panel, 3044 - Supplementary cover door, 305 - Telescopic member, 306 - Support plate, 307 - Spring, 308 - Protective sleeve, 4 - Temperature sensor, 5 - Self-fusing cooling particles, 501 - Rubber film, 502 - Ice cubes, 503 - Capillary pores, 504 - Outer membrane layer, 505 - Filling layer, 506 - Reinforcing belt, 507 - Water-soluble film. Detailed implementation manners

[0047] To enable those skilled in the art to better understand the technical solutions in the present invention, the following will clearly and completely describe the technical solutions in the embodiments of the present invention in conjunction with the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. All other embodiments obtained by those of ordinary skill in the art based on the embodiments of the present invention without creative efforts shall fall within the protection scope of the present invention.

[0048] As Figure 1 shown, a hazard factor screening system for cell-cultured meat food in an embodiment of the present invention includes a sensing unit, an execution unit, an analysis unit, and a control unit.

[0049] Among them, the sensing unit includes an integrated sensor and a live cell imaging device, and the integrated sensor is used to collect the cell-cultured meat culture environment in real time.

[0050] Specifically, the integrated sensor integrates a pH sensor, a dissolved oxygen sensor, and a temperature sensor on the same chip, and is used to monitor the pH value, dissolved oxygen content, and temperature of the cell-cultured meat culture environment in real time, so as to realize the dynamic assessment and early warning of hazard factors. At the same time, a graphene quantum dot sensor is also integrated on the chip to improve the detection sensitivity to the ppb level.

[0051] In addition, the live cell imaging device is used to dynamically track the cell growth state. The live cell imaging device is a CellAssist 50 system. The CellAssist 50 system uses label-free high-throughput imaging, can simultaneously monitor 50 microplates, perform more than 100-layer scans, and has a Z-axis imaging depth of up to 4 mm; the CellAssist 50 system supports two high-resolution imaging modes of bright field and phase contrast, is equipped with 4x, 10x, and 20x lenses, and has a fast imaging speed; it has a unique Z-axis imaging function: CellAssist 50 can perform label-free 3D imaging, capture clear images of more than 100 focal planes, and each focal plane is 2 μm to 50 μm apart; it can be compatible with a variety of samples; it has powerful analysis capabilities: CellAssist 50 can perform rapid big data analysis, output indicators such as cell fusion rate curve, number of cell clusters, doubling time, IC50, etc., and it also has a clone tracking function, which can trace the origin of each clone cluster and manage the data of multiple plates at the same time.

[0052] The CellAssist 50 system in this application can automatically scan the cell morphology changes every 15 minutes and give early warning of early apoptosis or abnormal proliferation. Through the mutual cooperation of the integrated sensor and the live cell imaging device, it is possible to realize the real-time monitoring of the cell-cultured meat culture environment and also to detect cell metabolic foreign substances such as volatile basic nitrogen and histamine.

[0053] As Figure 1 shown, the execution unit is used to collect and detect sample data according to a preset execution.

[0054] Among them, the execution unit includes a preliminary screening module and a re-inspection module. The preliminary screening module is used to quickly detect the microbial and chemical indicators of cultured meat. For example, a device that can obtain the structure within 30 minutes is used to improve the efficiency of preliminary screening detection.

[0055] Preferably, the device of the preliminary screening module in this application is a rapid detector for diseased meat. The rapid detector for diseased meat adopts a four-wavelength cold light source and a rotating colorimetric cell design, and has the characteristics of high efficiency, accuracy, real-time monitoring, simple operation and versatility, which is convenient for quickly detecting cultured meat.

[0056] In addition, the re-inspection module is used to conduct in-depth laboratory analysis on the samples that fail the preliminary screening. The re-inspection module conducts in-depth analysis on the samples that fail the preliminary screening based on the whole genome sequencing technology. The whole genome sequencing technology can comprehensively screen unknown pathogenic microorganisms and trace the pollution pathway.

[0057] Of course, other devices can also be used for detection by the execution unit, including but not limited to microbial pathogen detectors, multi-parameter food safety rapid detectors, X-ray detection, etc. The detection scope of the execution unit covers microbial pollution detection (such as Salmonella, Escherichia coli, Listeria, etc.), chemical residue and toxin analysis detection (such as antibiotics, hormones, mycotoxins, endotoxins, etc.) and physical hazard (such as cell culture scaffold materials, metal particles, etc.) screening to ensure the screening effect of cultured meat.

[0058] As Figure 1 shown, the analysis unit analyzes and processes the data information sent by the sensing unit and the execution unit based on the AI data analysis platform.

[0059] Among them, the AI data analysis platform constructs a detection database by combining machine learning algorithms and Internet of Things technology. Based on the historical data in the database, it realizes the dynamic assessment of risk factors and forms a risk heat map (such as the location of high pollution areas). Staff can quickly understand the current quality of cultured meat and the subsequent spoilage expectation according to the risk heat map, which is convenient for staff to make timely early warning preparations.

[0060] The machine learning algorithm can perform unsupervised learning and deep learning. For example, in unsupervised learning, the microbial community type is identified through clustering (such as Dirichlet multinomial mixture model) to assist in discovering environmentally sensitive species. In deep learning, such as when processing high-throughput sequencing data, the potential relationship between the microbiome and the environment is mined through a convolutional neural network (CNN).

[0061] As Figure 1As shown, the control unit dynamically adjusts the execution unit according to the analysis result of the analysis unit and triggers a traceability warning.

[0062] Among them, the control unit includes a traceability platform. The control unit optimizes the detection cycle of the execution unit in combination with the dynamic evaluation result, and feeds back the unqualified result of the sample to the traceability platform to locate the pollution source using the traceability platform.

[0063] For example, a cell-cultured meat enterprise uses the re-inspection module equipment to screen out that the residual gentamicin (the concentration exceeds the standard by 3 times) in a batch of cell-cultured meat culture medium, and promptly terminates production.

[0064] Another example is that a cell-cultured meat enterprise screens out that a batch of cell-cultured meat products contains 0.5m metal particles through X-ray, traces it back to the wear of the stirring paddle of the reaction tank, and promptly replaces the equipment to avoid losses.

[0065] In addition, the control unit can also be equipped with a remote update function and multi-terminal data synchronization, such as 4G / 5G / Bluetooth / Wi-Fi transmission, etc., which is convenient for staff to view relevant data and assist staff in making relevant decisions. If there are still problems with the re-inspected cell-cultured meat samples, corresponding treatment measures and methods will be provided.

[0066] Such as Figures 2 to 10 As shown, a screening device for harmful factors of cell-cultured meat food includes a rapid detector for diseased meat. The rapid detector for diseased meat is applied to the screening system for harmful factors of cell-cultured meat food. The rapid detector for diseased meat includes: a main body 1 of the diseased meat detector, a fixing frame 2, and a number of re-inspection sample temporary storage mechanisms 3.

[0067] Among them, a pair of connecting rods 201 are fixedly connected to the fixing frame 2. The fixing frame 2 is installed on the side wall of the main body 1 of the diseased meat detector through the connecting rods 201, and a number of installation slots are provided on the fixing frame 2. The re-inspection sample temporary storage mechanism 3 can be temporarily placed in the installation slot so as to place the cell-cultured meat samples that are unqualified in the preliminary screening by the main body 1 of the diseased meat detector into the re-inspection sample temporary storage mechanism 3 for temporarily storing the cell-cultured meat samples that are unqualified in the preliminary screening, avoiding the problem of deterioration of the cell-cultured meat samples that are unqualified in the preliminary screening and facilitating subsequent re-inspection.

[0068] Such as Figures 2 to 10 As shown, a number of re-inspection sample temporary storage mechanisms 3 are respectively arranged in a number of installation slots. The re-inspection sample temporary storage mechanism 3 is used to temporarily store the cell-cultured meat samples that are unqualified in the preliminary screening to avoid the samples from deteriorating due to external environmental factors.

[0069] Among them, the re-inspection sample temporary storage mechanism 3 includes a temporary storage tank 301. A fixed outer ring 3011 is connected to the outer wall of the temporary storage tank 301. The diameter of the fixed outer ring 3011 is greater than the diameter of the installation groove, and the fixed outer ring 3011 contacts the upper end surface of the fixing frame 2. When the temporary storage tank 301 is placed in the installation groove, the fixed outer ring 3011 contacts the upper end surface of the fixing frame 2 to support the temporary storage tank 301.

[0070] In addition, a fixed inner ring 3012 is provided on the inner wall of the temporary storage tank 301. A number of fixed columns are connected between the fixed inner ring 3012 and the inner wall of the temporary storage tank 301. The inner diameter of the fixed inner ring 3012 is greater than the outer diameter of the storage tube 302. The fixed inner ring 3012 is used to stabilize the storage tube 302, prevent the storage tube 302 from moving in the temporary storage tank 301, and ensure the storage of the initially screened unqualified samples in the storage tube 302.

[0071] Specifically, a storage tube 302 and a heat preservation liquid 303 are provided in the temporary storage tank 301. The storage tube 302 is arranged in the heat preservation liquid 303. The heat preservation liquid 303 is used to reduce the heat preservation of the initially screened unqualified samples in the storage tube 302 and prevent the initially screened unqualified samples from deteriorating due to excessive temperature.

[0072] Preferably, the entire temporary storage tank 301 is made of heat-insulating material to prevent the heat preservation liquid 303 from being affected by the external environment. The storage tube 302 is made of heat-conducting material. The heat preservation liquid 303 can be used to cool the initially screened unqualified samples in the storage tube 302. At the same time, the heat preservation liquid 303 is ice water, which is convenient for obtaining materials.

[0073] As Figures 2 to 10 shown, a end cap 304 is threadedly connected to the temporary storage tank 301 for installing the storage tube 302 in the temporary storage tank 301.

[0074] Among them, a top cover 3041 is connected to the inner top wall of the end cap 304. The top cover 3041 contacts the top wall of the storage tube 302 to further fix the storage tube 302, prevent the storage tube 302 from shifting in the temporary storage tank 301, and ensure the safety of the initially screened unqualified samples in the storage tube 302.

[0075] In addition, a cavity is provided in the end cap 304. A plurality of self-fusing cooling particles 5 are provided in the cavity. The particle size of the self-fusing cooling particles 5 is smaller than the inner diameter of the release tube 3042. When the heat preservation liquid 303 has a temperature rise, the self-fusing cooling particles 5 are used to reduce the temperature of the heat preservation liquid 303, ensure the low-temperature preservation effect of the heat preservation liquid 303 on the initially screened unqualified samples in the storage tube 302, prevent the initially screened unqualified samples from deteriorating during the re-inspection transportation, and further ensure the re-inspection effect of the subsequent initially screened unqualified samples.

[0076] Preferably, a heat-insulating layer is provided on the inner wall of the cavity to prevent the self-fusing cooling particles 5 from being affected by the external temperature.

[0077] Specifically, a release pipe 3042 is connected to the end cap 304. The cavity is connected to the inside of the temporary storage tank 301 through the release pipe 3042. The self-melting cooling particles 5 in the cavity can enter the temporary storage tank 301 through the release pipe 3042 and enter the heat preservation liquid 303.

[0078] Preferably, a control valve is installed on the release pipe 3042 to control the on-off of the release pipe 3042.

[0079] In addition, a panel 3043 is installed on the outer top wall of the end cap 304. The control valve is electrically connected to the panel 3043. The opening state of the control valve can be controlled by using the panel 3043. At the same time, the panel 3043 can also display the temperature of the heat preservation liquid 303, which is convenient for the staff to manually control the control valve.

[0080] Furthermore, a supplementary cover door 3044 is provided on the side wall of the end cap 304. The supplementary cover door 3044 corresponds to the cavity, which is convenient for supplementing the self-melting cooling particles 5 into the cavity.

[0081] As Figures 2 to 10 shown, a telescopic member 305 is installed on the bottom wall of the temporary storage tank 301. The free end of the telescopic member 305 is connected to a support plate 306. The storage pipe 302 is placed on the support plate 306. The support plate 306 is used to support the storage pipe 302. The storage pipe 302 is fixed in the temporary storage tank 301 under the combined action of the top cover 3041, the telescopic member 305 and the support plate 306, ensuring the transportation safety of the initially screened unqualified samples in the storage pipe 302.

[0082] Among them, a spring 307 is provided outside the telescopic member 305, which is used to apply a force to the support plate 306 and can fix the storage pipe 302 in the temporary storage tank 301 under the combined action of the top cover 3041, ensuring the transportation safety of the initially screened unqualified samples in the storage pipe 302.

[0083] In addition, a protective sleeve 308 is provided outside the spring 307. The protective sleeve 308 is arranged between the bottom wall of the temporary storage tank 301 and the support plate 306. The protective sleeve 308 is used to protect the telescopic member 305 and the spring 307, avoiding the influence of the heat preservation liquid 303 on the telescopic member 305 and the spring 307.

[0084] As Figures 2 to 10As shown, a temperature sensor 4 is installed on the inner wall of the temporary storage tank 301. The temperature sensor 4 is located below the fixed inner ring 3012 and is electrically connected to the panel 3043. The temperature sensor 4 is used to monitor the temperature of the heat preservation liquid 303. If the temperature of the heat preservation liquid 303 approaches 10°C, it will send a signal to the panel 3043. The panel 3043 controls the opening of the control valve, and the self-melting cooling particles 5 in the cavity enter the temporary storage tank 301 through the release pipe 3042 and then enter the heat preservation liquid 303. The self-melting cooling particles 5 are used to reduce the temperature of the heat preservation liquid 303, so that the temperature of the heat preservation liquid 303 drops to about 4°C, thereby avoiding problems such as deterioration of the initially screened unqualified samples in the storage pipe 302 during the re-inspection transportation and ensuring the re-inspection effect of the initially screened unqualified samples in the follow-up.

[0085] As Figures 2 to 10 shown, the self-melting cooling particles 5 include a rubber film 501. The rubber film 501 is used to contain ice cubes 502. Ice cubes 502 are provided inside the rubber film 501. The ice cubes 502 can be used to reduce the temperature of the heat preservation liquid 303 to ensure the continuous low-temperature preservation effect of the heat preservation liquid 303 on the initially screened unqualified samples in the storage pipe 302.

[0086] Among them, a number of capillary pores 503 are provided on the side wall of the rubber film 501. When the self-melting cooling particles 5 do not enter the heat preservation liquid 303, the rubber film 501 shrinks under the action of the ice cubes 502. At this time, the aperture of the capillary pores 503 becomes smaller and is blocked, that is, the ice cubes 502 will not pass through the capillary pores 503. When the self-melting cooling particles 5 enter the heat preservation liquid 303, the rubber film 501 unfolds, the aperture of the capillary pores 503 becomes larger, and the ice cubes 502 can pass through the capillary pores 503.

[0087] In addition, an outer film layer 504 is provided outside the rubber film 501. The outer film layer 504 is used to protect the filling layer 505. A filling layer 505 is provided between the rubber film 501 and the outer film layer 504. When the filling layer 505 comes into contact with the heat preservation liquid 303, bubbles will be formed. The bubbles can be used to mix the heat preservation liquid 303, so that the temperature of the heat preservation liquid 303 is kept uniform and the temperature difference is avoided.

[0088] Preferably, the filling layer 505 is an effervescent tablet. The effervescent tablet will produce bubbles when it meets water. The bubbles can be used to mix the heat preservation liquid 303 and avoid the temperature difference in the heat preservation liquid 303.

[0089] Specifically, a reinforcing belt 506 is provided inside the filling layer 505 to increase the integrity of the filling layer 505 and avoid the problem of looseness when the filling layer 505 is installed outside the capillary pores 503.

[0090] Preferably, both the outer film layer 504 and the reinforcing belt 506 are made of cloth material.

[0091] In addition, a water-soluble film 507 is provided outside the outer membrane layer 504. The water-soluble film 507 is used to protect the filling layer 505 and prevent the self-fusing cooling particles 5 from reacting in the cavity. The water-soluble film 507 can dissolve in the heat preservation liquid 303, enabling the heat preservation liquid 303 to react with the filling layer 505 to generate bubbles, and using the bubbles to mix the heat preservation liquid 303 to avoid temperature differences in the heat preservation liquid 303.

[0092] As Figures 2 to 10 shown, an auxiliary decision-making system is also provided in the main body 1 of the diseased meat detector. The auxiliary decision-making system can send relevant auxiliary suggestions to the staff. For example, for the cell-cultured meat that passes the initial screening, suggestions on the storage time and storage method are provided, and at the same time, suggestions on the best consumption time can be provided. For the samples that fail the initial screening, the samples can be temporarily stored in the re-inspection sample temporary storage institution 3 for subsequent re-inspection.

[0093] During specific use, the main body 1 of the diseased meat detector is used to conduct an initial screening on the cell-cultured meat sample. If the sample passes the initial screening, the auxiliary decision-making system can provide suggestions on the storage time, storage method, and best consumption time to the staff.

[0094] If the initially screened sample is unqualified, the sample needs to be temporarily stored in the re-inspection sample temporary storage institution 3 for subsequent re-inspection, so as to trace and locate.

[0095] When using the re-inspection sample temporary storage institution 3 to store the initially screened unqualified samples, first remove the end cap 304 from the temporary storage tank 301, take out the storage tube 302, place the initially screened unqualified sample in the storage tube 302, cover the cap of the storage tube 302, and then place the storage tube 302 in the temporary storage tank 301. One end of the storage tube 302 passes through the fixed inner ring 3012 and is placed on the support plate 306, and then the end cap 304 is reinstalled. The top cover 3041 squeezes the storage tube 302, and with the cooperation of the telescopic member 305, the support plate 306, and the spring 307, the storage tube 302 can be fixed in the temporary storage tank 301. At the same time, the heat preservation liquid 303 in the temporary storage tank 301 is used to store the initially screened unqualified samples in the storage tube 302 at a low temperature to prevent the initially screened unqualified samples from deteriorating due to temperature.

[0096] The temperature sensor 4 can be used to monitor the temperature of the heat preservation liquid 303. If the temperature of the heat preservation liquid 303 approaches 10 °C, a signal will be sent to the panel 3043, and the panel 3043 controls the opening of the control valve. The self-fusing cooling particles 5 in the cavity enter the temporary storage tank 301 through the release tube 3042 and into the heat preservation liquid 303. The self-fusing cooling particles 5 are used to reduce the temperature of the heat preservation liquid 303, so that the temperature of the heat preservation liquid 303 drops to about 4 °C, thereby preventing the initially screened unqualified samples in the storage tube 302 from deteriorating during re-inspection transportation and ensuring the re-inspection effect of the subsequent initially screened unqualified samples.

[0097] When the self-fusing cooling particles 5 come into contact with the heat preservation liquid 303, the water-soluble film 507 dissolves in the heat preservation liquid 303. At this time, the filling layer 505 contacts the heat preservation liquid 303 through the outer film layer 504. The filling layer 505 reacts with the heat preservation liquid 303 to generate bubbles. At the same time, the rubber film 501 can unfold under the action of the heat preservation liquid 303, making the pore diameter of the capillary pores 503 larger. The ice cubes 502 can be discharged through the capillary pores 503 and enter the heat preservation liquid 303. With the cooperation of the bubbles, the heat preservation liquid 303 can be cooled evenly without temperature difference, and thus the heat preservation effect of the heat preservation liquid 303 on the initially screened unqualified samples in the storage tube 302 can be continuously ensured, avoiding the deterioration problem of the initially screened unqualified samples during the re-inspection transportation and ensuring the accuracy of the subsequent re-inspection.

[0098] For those skilled in the art, it is obvious that the present invention is not limited to the details of the above exemplary embodiments, and the present invention can be implemented in other specific forms without departing from the spirit or basic characteristics of the present invention. Therefore, from any point of view, the embodiments should be regarded as exemplary and non-limiting. The scope of the present invention is defined by the appended claims rather than the above description. Therefore, all changes falling within the meaning and scope of the equivalent elements of the claims are intended to be included in the present invention. Any reference signs in the claims should not be regarded as limiting the claimed claim.

[0099] In addition, it should be understood that although this specification is described according to embodiments, not every embodiment only contains an independent technical solution. This narrative way of the specification is only for clarity. Those skilled in the art should regard the specification as a whole, and the technical solutions in each embodiment can also be appropriately combined to form other embodiments that can be understood by those skilled in the art.

Claims

1. A cell cultured meat food hazard factor screening system, characterized in that: include: A sensing unit, the sensing unit comprising an integrated sensor and a live cell imaging device, the integrated sensor being used to collect the cell culture environment in real time, and the live cell imaging device being used to dynamically track the cell growth state; An execution unit, the execution unit is used to execute sample data collection and detection according to a preset; An analysis unit, which analyzes and processes data information sent by the perception unit and the execution unit based on the AI ​​data analysis platform; and a control unit, which dynamically adjusts the execution unit according to the analysis result of the analysis unit and triggers a traceability warning; Among them, the execution unit includes a primary screening module and a re-inspection module. The primary screening module is used to quickly detect the microbial and chemical indicators of cell-cultured meat, and the re-inspection module is used to conduct in-depth laboratory analysis on samples that fail the primary screening.

2. A cell cultured meat food hazard factor screening system according to claim 1, characterized in that: The integrated sensor is used to monitor the pH value, dissolved oxygen content and temperature of the cell culture environment in real time. The living cell imaging device is the CellAssist 50 system, which automatically scans cell morphology changes every 15 minutes to warn of early apoptosis or abnormal proliferation.

3. The cell cultured meat food hazard factor screening system according to claim 1, characterized in that: The initial screening module equipment is a diseased meat rapid detector, and the re-inspection module performs in-depth analysis of samples that fail the initial screening based on whole genome sequencing technology.

4. The cell cultured meat food hazard factor screening system according to claim 1, characterized in that: The AI ​​data analysis platform combines machine learning algorithms and Internet of Things technologies to build a detection database, realizes dynamic evaluation of risk factors based on historical data in the database, and forms a risk heat map.

5. The cell cultured meat food hazard factor screening system according to claim 4, characterized in that: The control unit includes a traceability platform. The control unit optimizes the detection cycle of the execution unit in combination with the dynamic evaluation results, and feeds back the unqualified sample results to the traceability platform, and uses the traceability platform to locate the source of pollution.

6. A cell cultured meat food hazard factor screening device, comprising a diseased meat rapid detector, the diseased meat rapid detector is applied to the cell cultured meat food hazard factor screening system according to any one of claims 1 to 5, characterized in that: The diseased meat rapid detector comprises: Diseased meat detector body; A fixing frame, a pair of connecting rods are fixedly connected to the fixing frame, the fixing frame is installed on the side wall of the diseased meat detector body through the connecting rods, and a plurality of mounting grooves are provided on the fixing frame; A plurality of temporary storage mechanisms for re-examination samples are respectively arranged in the plurality of the installation grooves. The temporary storage mechanisms for re-examination samples include temporary storage tanks. The temporary storage tanks are provided with storage tubes and insulation liquid. The storage tubes are arranged in the insulation liquid. The temporary storage tanks are threadedly connected with end covers.

7. The cell cultured meat food hazard factor screening device according to claim 6, characterized in that: The outer wall of the temporary storage tank is connected to a fixed outer ring, the diameter of the fixed outer ring is larger than the diameter of the mounting groove, the fixed outer ring is in contact with the upper end surface of the fixing frame, the inner wall of the temporary storage tank is provided with a fixed inner ring, a plurality of fixed columns are connected between the fixed inner ring and the inner wall of the temporary storage tank, and the inner diameter of the fixed inner ring is larger than the outer diameter of the storage tube.

8. The cell cultured meat food hazard factor screening device according to claim 7, characterized in that: The inner top wall of the end cover is connected with a top cover, and the top cover contacts the top wall of the storage tube. A cavity is provided in the end cover, and a plurality of self-melting cooling particles are provided in the cavity. A release pipe is connected to the end cover, and the cavity is connected with the interior of the temporary storage tank through the release pipe, and a control valve is installed on the release pipe.

9. The cell cultured meat food hazard factor screening device according to claim 8, characterized in that: A panel is installed on the outer top wall of the end cover, the control valve is electrically connected to the panel, and a supplementary cover door is provided on the side wall of the end cover, and the supplementary cover door corresponds to the cavity.

10. The cell cultured meat food hazard factor screening device according to claim 9, characterized in that: A temperature sensor is installed on the inner wall of the temporary storage tank. The temperature sensor is arranged on the lower side of the fixed inner ring. The temperature sensor is electrically connected to the panel.

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