A portable fluorescence detector for rapid detection

Abstract

The application discloses a portable fluorescence detector for rapid detection, which comprises a box body and a top cover. A fixed hinge is arranged between the box body and the top cover. A control panel and an observation port are arranged on the outer surface of the top cover. The observation port is provided with an upper filter, which is used for observing the inside of the box body. A hole plate is arranged in the box body. The front end of the hole plate is provided with a guide rail. The surface of the guide rail is provided with a first sliding block and a second sliding block. The fluorescence of the sample in the sample cell can be directly detected through the dichroic mirror, the filter support and the laser structure. The detection method is simple and convenient to adjust. The structure is divided into three parts. When the position is wrong, the user can adjust it by himself without repeated maintenance and fine adjustment.

Description

TECHNICAL FIELD

[0001] The present application relates to the field of trace detection, in particular to a portable fluorescence detector for rapid detection. BACKGROUND

[0002] Cancer is one of the diseases with high mortality rate globally, and early detection and timely diagnosis are crucial for improving cure rate and survival rate. Traditional cancer screening methods, such as imaging examination and tissue biopsy, usually require professional equipment, are expensive, and are complex to operate, making it difficult to popularize in primary medical environment. Therefore, there is an urgent need for an efficient, convenient and economical screening tool that can sensitively detect potential markers in the early stage of cancer

[0003] The fluorescence detector has strong clinical detection effect in trace detection of cancer, especially the targeted combination of fluorescence probes and tumor markers (such as ctDNA, exosomes), which can realize ultra-trace analysis and have certain guiding effect on the disease condition.

[0004] With the progress of molecular biology technology, early screening of cancer has gradually shifted to detection of specific disease markers. However, existing portable fluorescence instruments still have some challenges in practical application, especially when the internal adjustment or operation of the device is required. Due to the high integration of the internal components, the adjustment process is complex, and mis-touch or mis-touch often occurs, which makes the portable fluorescence instrument unable to use. SUMMARY

[0005] The technical problem to be solved by the present application is to overcome the defects of the prior art and provide a portable fluorescence detector for rapid detection, which mainly solves the technical problems of too many internal components, complex adjustment process and inconvenience of carrying.

[0006] In order to solve the above technical problems, the present application provides the following technical solutions:

[0007] The present application provides a portable fluorescence detector for rapid detection, which comprises a fluorescence detector including a box body and a top cover, and a fixed hinge is installed between the box body and the top cover, characterized in that:

[0008] A control panel and an observation port are installed on the outer surface of the top cover, and the observation port is provided with an upper filter for observing the inside of the box body.

[0009] The box body is internally provided with a hole plate, the front end of the hole plate is provided with a guide rail, the surface of the guide rail is provided with a first sliding block and a second sliding block, the upper side of the first sliding block is provided with a first adjustable three-axis support, the end of the first adjustable three-axis support is provided with a laser, the upper side of the second sliding block is provided with a second adjustable three-axis support, the end of the second adjustable three-axis support is provided with a dichroic clamp, the end of the dichroic clamp is provided with a dichroic mirror, the bottom side of the dichroic mirror is provided with a sample cell, the bottom of the sample cell is provided with a heating pad, the heating pad is connected to a control panel, a filter holder is arranged between the dichroic mirror and the laser, and the filter holder is arranged on the surface of the hole plate; the dichroic mirror, the filter holder and the laser are located on the same horizontal line, and the angle between the dichroic mirror and the plane is 45 degrees.

[0010] Preferably, the upper surfaces of the first sliding block and the second sliding block are provided with an extension piece and a bolt, the bolt is used for fixing the extension piece to the surfaces of the first sliding block and the second sliding block, the bottoms of the first adjustable three-axis support and the second adjustable three-axis support are arranged on the surface of the extension piece, and the extension piece is used for adjusting the angle in the horizontal direction.

[0011] Preferably, the laser is a 488 nm, 50 mW semiconductor laser, and the surface of the filter holder is provided with a 480 nm band-pass filter.

[0012] Preferably, the size of the sample cell is 2*2*2 cm, and the viewing port and the dichroic mirror are vertically corresponding.

[0013] Preferably, the upper filter is an emission filter, the upper filter is 535 nm, the dichroic mirror is used for reflecting 450-500 nm and transmitting 515-800 nm.

[0014] Preferably, the control panel comprises a micro control chip or a PLC chip, and the control panel is electrically connected to the heating pad and the laser respectively.

[0015] Preferably, the front part of the box body is provided with a clamping groove, the inner side of the clamping groove is provided with a plug buckle, and the plug buckle is used for buckling to the top cover.

[0016] Preferably, the inner side of the top cover is provided with a foam pad, the foam pad is used for placing the filter holder and the dichroic mirror, the bottom of the top cover is provided with a blocking plate, a mounting type hinge is connected between the blocking plate and the top cover, the inner wall of the top cover is provided with a buckling groove, the inner surface of the blocking plate is provided with a buckling block, and the buckling block and the buckling groove are correspondingly arranged.

[0017] Preferably, the front part of the blocking plate is provided with a mounting groove, the position of the mounting groove corresponds to the plug buckle, the width of the mounting groove is greater than that of the plug buckle, the rear part of the blocking plate is provided with a rotating shaft, the surface of the rotating shaft is provided with an inclined support rod, and the front surface of the box body is provided with a limiting groove.

[0018] Preferably, the mounting hinge comprises an outer fixing sleeve and an inner rotating shaft, the inner rotating shaft is arranged in the outer fixing sleeve in an axial sleeve manner, the inner rotating shaft is provided with a connecting piece, and the connecting piece is connected with the blocking plate; the outer fixing sleeve is provided with a sliding groove, the sliding groove is in an L shape on the surface of the outer fixing sleeve, and the connecting piece structure is arranged in correspondence with the sliding groove.

[0019] Compared with the prior art, the application has the following beneficial effects:

[0020] 1: The application realizes direct fluorescence detection of samples in a sample cell through the two-color mirror, the filter holder and the laser structure, the detection method is simple, and the user can adjust it conveniently, the structure is only divided into three parts, even if the structure is misaligned on site, the user can also adjust and use it by himself, without repeated maintenance and fine adjustment operations.

[0021] 2: The application further comprises a blocking plate structure, the blocking plate can be used to place multiple samples on it in a field environment to achieve rapid detection, and when the blocking plate is not used, the parts inside the foam pad can be fixed to avoid damage of the internal glass parts due to vibration when the box is moved. DETAILED DESCRIPTION

[0022] The accompanying drawings are used to provide a further understanding of the application, and constitute a part of the specification, and are used to explain the application together with embodiments of the application, and do not constitute a limitation to the application. In the drawings:

[0023] Figure 1 is a schematic view of the overall structure of example 1;

[0024] Figure 2 is an expanded view of the box structure of example 1;

[0025] Figure 3 is a schematic view of the sample cell structure of example 1;

[0026] Figure 4 is a schematic view of the filter holder structure of example 1;

[0027] Figure 5 is a schematic view of the overall structure of example 2;

[0028] Figure 6 is a split view of the mounting hinge structure of example 2;

[0029] In the diagram: 1. Fluorescence detector; 101. Housing; 102. Top cover; 103. Fixed hinge; 104. Control panel; 105. Observation port; 106. Upper filter; 2. Perforated plate; 201. Guide rail; 202. First slider; 203. Bolt; 204. Extension; 205. First adjustable triaxial support; 206. Laser; 207. Second slider; 208. Second adjustable triaxial support; 209. Two-color clamp; 3. Two-color... 4. Filter holder; 5. Sample cell; 501. Heating pad; 6. Fixing body; 7. Barrier plate; 701. Mounting hinge; 702. Foam pad; 703. Sealing strip; 704. Fastening groove; 705. Fastening block; 706. Mounting groove; 707. Diagonal brace; 708. Rotating shaft; 8. Slot; 801. Snap-on; 802. Limiting groove; 9. Outer fixing sleeve; 901. Sliding groove; 902. Inner rotating shaft; 903. Connecting piece. Detailed Implementation

[0030] The preferred embodiments of the present invention will be described below with reference to the accompanying drawings. It should be understood that the preferred embodiments described herein are for illustration and explanation only and are not intended to limit the present invention.

[0031] Example 1

[0032] like Figures 1-4 As shown, the present invention provides a portable fluorescence detector for rapid detection. The fluorescence detector 1 includes a housing 101 and a top cover 102, and a fixed hinge 103 is installed between the housing 101 and the top cover 102, wherein:

[0033] A control panel 104 and an observation port 105 are installed on the outer surface of the top cover 102. The observation port 105 is provided with an upper filter 106, which is used to observe the inside of the housing 101.

[0034] An orifice plate 2 is installed inside the housing 101. A guide rail 201 is provided at the front end of the orifice plate 2. A first slider 202 and a second slider 207 are installed on the surface of the guide rail 201. A first adjustable triaxial support 205 is installed on the upper side of the first slider 202. A laser 206 is installed at the end of the first adjustable triaxial support 205. A second adjustable triaxial support 208 is installed on the upper side of the second slider 207. A dichroic clamp 209 is installed at the end of the second adjustable triaxial support 208. A dichroic mirror 3 is installed at the end of the dichroic clamp 209. A sample cell 5 is provided on the bottom side of the dichroic mirror 3. A heating pad 501 is installed at the bottom of the sample cell 5 and is connected to the control panel 104. A filter holder 4 is provided between the dichroic mirror 3 and the laser 206 and is installed on the surface of the orifice plate 2. The dichroic mirror 3, the filter holder 4 and the laser 206 are located on the same horizontal line, and the dichroic mirror 3 has an angle of 45 degrees with the plane.

[0035] The overall structure is designed as a box 101. After closing the top cover 102, the inside of the box 101 is in a lightless state. The first slider 202 and the second slider 207 can move left and right on the guide rail 201 to adjust the distance, and the first adjustable three-axis support 205 and the second adjustable three-axis support 208 respectively complete the angle adjustment of the dichroic clamp 209 and the laser 206. After closing the box 101, the laser 206 is started by the control panel 104. The emitted laser is filtered by the excitation filter on the filter support 4 to obtain the specific wavelength light required for exciting the target fluorescent substance. Based on the deflection angle of the dichroic mirror 3, the excitation light and the emission light are effectively separated to reduce interference, so that the sample on the sample cell 5 emits fluorescence after absorbing light energy, and the fluorescence is reflected to the observation port 105 through the dichroic mirror 3, realizing the effects of simple integration and convenient carrying, and only 100 μL of sample volume is required to complete the detection. Because the laser light source with adjustable focus and the precise optical system are used, the device is not only convenient to carry, but also can efficiently detect cancer-related markers at low concentrations.

[0036] Further, the upper surfaces of the first slider 202 and the second slider 207 are respectively provided with an extension piece 204 and a bolt 203. The bolt 203 is used to fix the extension piece 204 to the surface of the first slider 202 and the second slider 207. The bottom of the first adjustable three-axis support 205 and the second adjustable three-axis support 208 is respectively installed on the surface of the extension piece 204. The extension piece 204 is used to adjust the angle in the horizontal direction. The laser 206 and the dichroic clamp 209 can adjust the included angle in the horizontal direction, so as to facilitate the fine adjustment operation in the box 101.

[0037] Further, the laser 206 is a 488 nm, 50 mW semiconductor laser, and the filter support 4 is provided with a 480 nm band-pass filter. The above structure is mainly used for accurately exciting specific fluorescent substances and optimizing optical signals. The combination optimizes the wavelength purity of the 488 nm laser through the 480 nm band-pass filter, ensures efficient excitation of the target fluorescence (such as GFP), and at the same time suppresses stray light, and is suitable for biomedical imaging, cell analysis and other scenes that require high-specificity fluorescent excitation.

[0038] Further, the size of the sample cell 5 is 2×2×2 cm, and the observation port 105 and the dichroic mirror 3 are vertically corresponding. The 2×2×2 cm sample cell 5 can accommodate 200 μL of sample, so as to facilitate the naked eye observation of the sample fluorescence at the observation port 105.

[0039] Furthermore, the upper filter 106 is an emission filter with a wavelength of 535 nm. The dichroic mirror 3 is used to reflect 450-500 nm and transmit 515-800 nm. The combination of the upper filter 106 and the dichroic mirror 3 is mainly used to achieve efficient fluorescence excitation and separation. The dichroic mirror 3 is used to reflect short-wavelength excitation light (such as 488 nm laser) and guide it to the sample to excite fluorescence, while allowing long-wavelength fluorescence (such as above 500 nm) to pass through and enter the detection end (such as the observation port). The upper filter 106 further filters the fluorescence signal, allowing only narrow-band light near 535 nm to pass through (e.g., 530-540 nm), blocking other wavelength interference (such as autofluorescence or stray light). The fluorescence emission wavelength is usually longer than the excitation light (e.g., GFP emits 509 nm). The dichroic mirror 3 allows fluorescence above 515 nm to pass through, preventing the excitation light from directly entering the detection end.

[0040] Furthermore, the control panel 104 includes a microcontroller chip or a PLC chip, and the control panel 104 is electrically connected to the heating pad 501 and the laser 206 respectively; a fixing body 6 is installed at the bottom of the filter bracket 4, and the fixing body 6 is installed on the surface of the perforated plate 2.

[0041] Working principle: During use, the sample to be tested is added to the EP tube, ensuring that the sample volume does not exceed 100 μL, and then added to the sample cell 5. The instrument is then turned on, and the heating pad 501 begins to heat to the required temperature, ensuring that the sample reacts under optimal temperature conditions. At this time, the excitation source of the laser 206 illuminates the sample through the 480 nm excitation filter. After absorbing the light energy, the sample emits fluorescence, and finally the fluorescence information is obtained through the observation port 105, so as to achieve efficient detection of cancer-related biomarkers at low concentrations. This device is not only highly integrated and portable, but also requires only a small amount of sample to complete the detection operation. At the same time, the device is easy to fine-tune internally and can effectively improve the fluorescence detection sensitivity and reduce background noise.

[0042] Example 2

[0043] The difference from Example 1 is that, as Figure 1 , 5 As shown in Figure 6, the front of the housing 101 is provided with a slot 8, and the inner side of the slot 8 is provided with a buckle 801, which is used to fasten to the top cover 102.

[0044] The top cover 102 has a foam pad 702 on the inside, which is used to place the filter holder 4 and the dichroic mirror 3. A barrier plate 7 is installed at the bottom of the top cover 102, and an installation hinge 701 is connected between the barrier plate 7 and the top cover 102. The inner wall of the top cover 102 has a snap-fit ​​groove 704, and a snap-fit ​​block 705 is installed on the inner surface of the barrier plate 7. The snap-fit ​​block 705 and the snap-fit ​​groove 704 are correspondingly set.

[0045] The front part of the blocking plate 7 is provided with a mounting groove 706, which is in position corresponding to the plug 801 and has a width greater than the plug 801. The rear part of the blocking plate 7 is provided with a rotating shaft 708, the surface of which is provided with an inclined support rod 707. The front surface of the box body 101 is provided with a limiting groove 802. The inner wall edge of the top cover 102 is provided with a sealing strip 703, which is provided in a U-shaped structure.

[0046] The mounting type hinge 701 comprises an outer fixed sleeve 9 and an inner rotating shaft 902, which is used to be axially sleeved with the outer fixed sleeve 9. The surface of the inner rotating shaft 902 is provided with a connecting piece 903, which is connected with the blocking plate 7. The surface of the outer fixed sleeve 9 is provided with a sliding groove 901, which is in an L-shaped structure on the surface of the outer fixed sleeve 9. The structure of the connecting piece 903 is correspondingly arranged with the sliding groove 901.

[0047] Specifically, the blocking plate 7 is arranged at the bottom of the top cover 102, and the cross section of the blocking plate 7 corresponds to the size of the clamping groove 8, so that the filter holder 4, the dichroic mirror 3 and the sample cell 5 and other components damaged due to vibration can be placed at the foam pad 702 and connected to the clamping groove 704 by the clamping block 705 of the blocking plate 7 when they are disassembled, so as to form the fixation of the foam pad 702. At the same time, the front part of the blocking plate 7 and the sealing strip 703 can seal between the top cover 102 and the box body 101, so that the box body 101 in the non-use state is not only in a sealed state, but also the internal parts can be protected.

[0048] In a further embodiment, the blocking plate 7 also has another use mode, that is, when the box body 101 needs to be used, the blocking plate 7 is directly rotated to the outside of the box body 101, as shown in Figure 5 At this time, the blocking plate 7 is rotated based on the rotating shaft 708 by the inclined support rod 707, until the inclined support rod 707 is inserted into the limiting groove 802. The mounting type hinge 701 can also form a fixed effect and is fixed to the mounting groove 706 of the blocking plate 7 in cooperation with the plug 801. At the same time, the surface of the blocking plate 7 is also provided with an inward recess, that is, after the deformation of the blocking plate 7, a small table plate of the box body 101 is formed to place a terminal device such as a mobile phone, a video camera or a recording terminal. The user mainly observes the internal sample fluorescence information from the observation port 105 on the side of the fixed hinge 103.

[0049] In further embodiments, the mounting hinge 701 is designed with a detachable structure of the outer fixed sleeve 9 and the inner rotating shaft 902, so that when the inner rotating shaft 902 is inside the outer fixed sleeve 9, the radially opened sliding groove 901 can allow the connecting piece 903 to rotate 180 degrees inside it, that is, when the blocking plate 7 is inside the box body 101, the connecting piece 903 is located in the middle of the sliding groove 901, at this time the inner rotating shaft 902 can be rotated at will; when the blocking plate 7 needs to be disassembled, only the connecting piece 903 is placed in the axial sliding groove 901 of the sliding groove 901, so that the blocking plate 7 can be directly disassembled from the top cover 102, only the plug-in buckle 801 and the mounting groove 706, and the inclined support rod 707 are used as a triangular support, so that when the top cover 102 is opened, the blocking plate 7 can move a certain distance to the fixed hinge 103 side due to the width of the mounting groove 706 being greater than the plug-in buckle 801, so that the inner rotating shaft 902 and the outer fixed sleeve 9 are arranged in front and back staggered, and the blocking plate 7 can be placed alone and does not affect the use of the top cover 102, so the surface of the blocking plate 7 can place multiple different samples, so as to realize the effect of convenient replacement of samples and rapid detection.

[0050] Finally, it should be noted that: the above only describes the preferred embodiments of the present application and is not intended to limit the present application. Although the present application has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions recorded in the foregoing embodiments or make equivalent replacements to some technical features. Any modification, equivalent replacement, improvement, etc. within the spirit and principles of the present application shall be included in the protection scope of the present application.

Claims

1. A portable fluorescence detector for rapid detection, the fluorescence detector (1) comprising a box (101) and a top cover (102), a fixed hinge (103) being installed between the box (101) and the top cover (102), characterized in that: an observation port (105) and a control panel (104) are installed on the outer surface of the top cover (102), the observation port (105) is provided with an upper filter (106) for observing the inside of the box (101); a hole plate (2) is installed in the box (101), the front end of the hole plate (2) is provided with a guide rail (201), the surface of the guide rail (201) is provided with a first sliding block (202) and a second sliding block (207), the upper side of the first sliding block (202) is provided with a first adjustable three-axis support (205), the end of the first adjustable three-axis support (205) is provided with a laser (206); the upper side of the second sliding block (207) is provided with a second adjustable three-axis support (208); the end of the second adjustable three-axis support (208) is provided with a dichroic clamp (209), the end of the dichroic clamp (209) is provided with a dichroic mirror (3), the bottom side of the dichroic mirror (3) is provided with a sample cell (5), the bottom of the sample cell (5) is provided with a heating pad (501), and the heating pad (501) is connected to the control panel (104), a filter support (4) is provided between the dichroic mirror (3) and the laser (206), and the filter support (4) is installed on the surface of the hole plate (2); the dichroic mirror (3), the filter support (4) and the laser (206) are located on the same horizontal line, and the angle between the dichroic mirror (3) and the plane is 45 degrees; a foam pad (702) is provided on the inner side of the top cover (102), the foam pad (702) is used for placing the filter support (4) and the dichroic mirror (3), a blocking plate (7) is installed at the bottom of the top cover (102), and a mounting hinge (701) is connected between the blocking plate (7) and the top cover (102); a buckling groove (704) is provided on the inner wall of the top cover (102), a buckling block (705) is installed on the inner surface of the blocking plate (7), and the buckling block (705) and the buckling groove (704) are correspondingly arranged; a clamping groove (8) is provided at the front of the box (101), an insertion buckle (801) is provided on the inner side of the clamping groove (8), and the insertion buckle (801) is used for buckling to the top cover (102); an installation groove (706) is provided at the front of the blocking plate (7), the installation groove (706) is correspondingly arranged at the position of the insertion buckle (801), and the width of the installation groove (706) is greater than that of the insertion buckle (801), a rotating shaft (708) is installed at the rear of the blocking plate (7), an inclined support rod (707) is installed on the surface of the rotating shaft (708), a limiting groove (802) is provided on the front surface of the box (101), and the inclined support rod (707) is used for being inserted into the limiting groove (802); a sealing strip (703) is provided on the edge of the inner wall of the top cover (102), and the sealing strip (703) is provided in a U-shaped structure.

2. The portable fluorometer for rapid detection according to claim 1, characterized in that, The upper surface of the first slider (202) and the second slider (207) is provided with an extension piece (204) and a bolt (203), the bolt (203) is used for fixing the extension piece (204) to the surface of the first slider (202) and the second slider (207), the bottom of the first adjustable three-axis support (205) and the second adjustable three-axis support (208) is mounted on the surface of the extension piece (204), and the extension piece (204) is used for adjusting the angle in the horizontal direction.

3. The portable fluorometer for rapid detection according to claim 1, characterized in that, The laser (206) is a 488 nm, 50 mW semiconductor laser, and the surface of the filter support (4) is provided with a 480 nm band-pass filter.

4. The portable fluorometer for rapid detection according to claim 1, characterized in that, The size of the sample cell (5) is 2*2*2 cm, and the observation port (105) and the dichroic mirror (3) are vertically corresponding.

5. The portable fluorometer for rapid detection according to claim 1, characterized in that, The upper filter (106) is an emission filter, and the upper filter (106) is 535 nm, the dichroic mirror (3) is used for reflecting 450-500 nm and transmitting 515-800 nm.

6. The portable fluorometer for rapid detection according to claim 1, wherein The control panel (104) includes a micro control chip or a PLC chip, the control panel (104) is electrically connected to the heating pad (501) and the laser (206) respectively, and the bottom of the filter support (4) is provided with a fixing body (6) mounted on the surface of the hole plate (2).

7. The portable fluorometer for rapid detection according to claim 1, wherein The mounting type hinge (701) includes an outer fixed sleeve (9) and an inner rotating shaft (902), the inner rotating shaft (902) is used for being axially sleeved with the outer fixed sleeve (9), the surface of the inner rotating shaft (902) is provided with a connecting piece (903), and the connecting piece (903) is connected with the blocking plate (7); the surface of the outer fixed sleeve (9) is provided with a sliding groove (901), the sliding groove (901) is L-shaped on the surface of the outer fixed sleeve (9), and the connecting piece (903) is correspondingly arranged with the sliding groove (901).

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