Angelica sinensis aqueous solution for destroying ampullaria gigas eggs and preparation method thereof

Abstract

The invention relates to the technical field of biopesticides, in particular to a levisticum aqueous solution for destroying ampullaria gigas eggs and a preparation method. The invention discloses a levisticum aqueous solution for destroying ampullaria gigas eggs, which comprises the following components in percentage by weight: 0.1-1% of a levisticum extract, 10-30% of a solvent, 5-10% of an emulsifier, 20-30% of an auxiliary agent, 1-2% of a defoaming agent and the balance of water, the preparation method of the levisticum aqueous solution comprises the following steps: mixing the levisticum extract and the solvent, adding the emulsifier, the assistant, the water and the defoaming agent, and mixing to obtain the levisticum aqueous solution. The added levisticum extract can prevent and treat ampullaria gigas eggs, and has the advantages of good diffusivity, good egg killing effect and low use cost.

Description

Technical Field

[0001] This application relates to the technical field of biological pesticides, and particularly relates to an angelica levisticum aqueous solution for destroying the eggs of Pomacea canaliculata and a preparation method thereof. Background Art

[0002] Pomacea canaliculata can harm aquatic crops, threaten aquatic organisms and has a strong reproductive ability. With global warming and its strong adaptability, the harm it causes has been increasing year by year. Almost all rivers and paddy fields in the south have Pomacea canaliculata, resulting in reduced yields of aquatic plants such as rice, lotus ponds, and water chestnuts. At the same time, in aquaculture fields such as fish ponds and shrimp ponds, Pomacea canaliculata competes with fish and shrimp for food and oxygen, leading to a significant reduction in the yields of aquatic organisms such as fish and shrimp. Currently, the main pesticides for killing Pomacea canaliculata are drugs such as niclosamide, which have good snail-killing effects and are safe for fish and shrimp, but cannot control the hatching of eggs and cannot kill snails at the root.

[0003] Currently, there are more than 10 kinds of active ingredients of plant-derived biological pesticides registered in China, such as matrine, nicotine, rotenone, pyrethrins, etc. Only 3 kinds of active ingredients, namely matrine, nicotine, and rotenone, are used to control spider mites.

[0004] Levisticum belongs to the Umbelliferae family and the genus Levisticum. It is native to western Asia and is widely cultivated in European and North American countries. China introduced it from Europe in 1957. Currently, it is planted in Hebei, Shandong, Henan, Inner Mongolia, Liaoning, Shaanxi, Shanxi, Jiangsu and other provinces and regions, and is used by the folk as a substitute for angelica. Some European countries include this product in the pharmacopoeia and use its roots for diuresis, stomach strengthening, expectoration, aromatic excitement, expelling wind and sweating, and treating gynecological diseases, neurological diseases, edema and chronic heart diseases. There is no report at home and abroad on the plant-derived biological pesticide developed with angelica levisticum oil as the mother liquor for destroying the eggs of Pomacea canaliculata.

[0005] Therefore, the development of plant-derived biological pesticides using angelica levisticum provides a new way for the comprehensive development and utilization of this medicinal plant. Summary of the Invention

[0006] In view of the deficiencies of the prior art, this application provides an angelica levisticum aqueous solution for destroying the eggs of Pomacea canaliculata and a preparation method thereof.

[0007] The purpose of this application is to provide a formula for a plant-derived aqueous solution that can destroy the eggs of Pomacea canaliculata and its preparation method; during the peak spawning period of Pomacea canaliculata, spraying the angelica levisticum aqueous solution evenly on the egg masses of Pomacea canaliculata can destroy the structure of the eggs of Pomacea canaliculata, making them unable to hatch, and the use cost is relatively low. Using this aqueous solution to control the eggs of Pomacea canaliculata can significantly reduce the number of times and dosage of chemical pesticides used, and effectively reduce the population of Pomacea canaliculata in river fields.

[0008] In the first aspect, this application provides an angelica levisticum aqueous solution for destroying the eggs of Pomacea canaliculata, adopting the following technical solution: An angelica sinensis aqueous solution for destroying the eggs of Pomacea canaliculata, by weight percentage, comprises 0.1%-1% of angelica sinensis extract, 10%-30% of solvent, 5%-10% of emulsifier, 20%-30% of auxiliary agent, 1%-2% of defoamer, and the balance is water.

[0009] This application discloses an angelica sinensis aqueous solution for destroying the eggs of Pomacea canaliculata. The added angelica sinensis extract can prevent and control the eggs of Pomacea canaliculata, and has the advantages of good diffusibility, good ovicidal effect, and low use cost. The angelica sinensis aqueous solution prepared in this application is a plant-derived ovicide, which can kill eggs and fundamentally solve the problem.

[0010] Preferably, the angelica sinensis aqueous solution, by weight percentage, comprises 0.1%-0.5% of angelica sinensis extract, 15%-20% of solvent, 8%-10% of emulsifier, 20%-25% of auxiliary agent, 1%-2% of defoamer, and the balance is water.

[0011] More preferably, the angelica sinensis aqueous solution, by weight percentage, is 0.2% of angelica sinensis extract, 18% of solvent, 10% of emulsifier, 20% of auxiliary agent, 2% of defoamer, and the balance is water.

[0012] Preferably, the solvent comprises at least one of isobutanol, isopropanol, dimethyl sulfoxide, dimethylformamide, and ether.

[0013] More preferably, the solvent is isopropanol.

[0014] Preferably, the emulsifier comprises at least one of calcium dodecylbenzenesulfonate, fatty alcohol polyoxyethylene ether, castor oil polyoxyethylene ether, Ethylan NS-500LQ nonionic hydroxy polyoxyethylene block copolymer, and sodium dioctyl sulfosuccinate.

[0015] More preferably, the emulsifier is calcium dodecylbenzenesulfonate.

[0016] Preferably, the auxiliary agent comprises at least one of lecithin, trisiloxane, octamethylcyclotetrasiloxane, and polyethylene glycol.

[0017] More preferably, the auxiliary agent is polyethylene glycol.

[0018] Preferably, the defoamer comprises at least one of isooctanol, polyol fatty acid ester, distearoylethylenediamine, and halogenated hydrocarbon.

[0019] More preferably, the defoamer is distearoylethylenediamine.

[0020] Preferably, the angelica sinensis extract is angelica sinensis oil.

[0021] By adopting the above technical solutions, in the present application, through the improvement of raw materials, Levisticum officinale oil is added. The Levisticum officinale oil contains active ingredients such as phthalide derivatives, volatile oils, coumarin compounds, and terpene compounds. The killing mechanism of the eggs of Pomacea canaliculata is as follows: (1) Insecticidal effect: The phthalide derivatives in Levisticum officinale oil can kill the eggs.

[0022] (2) Penetration effect: The volatile oil in Levisticum officinale oil can penetrate the outer protein layer of the egg membrane and destroy its structural integrity; (3) Enzymatic hydrolysis effect: The coumarin compounds in Levisticum officinale oil can activate or simulate the action of certain enzymes, decompose the chitin layer in the egg membrane, and further weaken the protective ability of the egg membrane; (4) Oxidation effect: The terpene compounds in Levisticum officinale oil will attack the double bond of the hydroxyl radical ·OH of the unsaturated fatty acid in the egg membrane, trigger an oxidation reaction, destroy the lipid structure of the inner layer of the egg membrane, and cause the egg membrane to lose its protective function.

[0023] The present application discloses a Levisticum officinale aqueous agent for destroying the eggs of Pomacea canaliculata. The added Levisticum officinale oil can prevent and control the eggs of Pomacea canaliculata, and has the advantages of good diffusibility, good egg-killing effect, and low use cost.

[0024] Preferably, the preparation method of the Levisticum officinale oil is as follows: The Levisticum officinale seeds are crushed into 60 meshes to obtain Levisticum officinale seed powder; The Levisticum officinale seed powder is mixed with a solvent, and ultrasonic extraction, filtration, and concentration are carried out to obtain Levisticum officinale oil.

[0025] Preferably, the mass-volume ratio of the Levisticum officinale seed powder to the solvent is (1-5) g:(2-6) ml.

[0026] In a specific feasible implementation scheme, the Levisticum officinale oil is extracted from the seeds of Levisticum officinale or the Levisticum officinale plant that can be planted on a large scale artificially.

[0027] In a specific feasible implementation scheme, the extraction process of the Levisticum officinale oil is as follows: The Levisticum officinale seeds (or Levisticum officinale plants) are crushed into 60 meshes to facilitate the release of active ingredients during the extraction process; the Levisticum officinale seed powder is mixed with a solvent and placed in an ultrasonic extractor. The cavitation effect and mechanical vibration of ultrasonic waves are used to accelerate the dissolution and release of active ingredients; an appropriate extraction time is set to ensure the full extraction of active ingredients; after the extraction is completed, the solid residue is removed by filtration, and the extract is concentrated to obtain a concentrated extract; the concentrated extract is dried to obtain Levisticum officinale oil.

[0028] In the second aspect, the present application provides a preparation method of a Levisticum officinale aqueous agent for destroying the eggs of Pomacea canaliculata, adopting the following technical solutions: A preparation method of Levisticum officinale water agent for destroying the eggs of Pomacea canaliculata, the steps of the preparation method are as follows: Weigh each raw material according to the formula; After mixing the Levisticum officinale extract and the solvent, then add an emulsifier, an auxiliary agent, water and an antifoaming agent for mixing to obtain the Levisticum officinale water agent.

[0029] The Levisticum officinale water agent prepared in this application is a plant-derived ovicide, which can kill eggs, fundamentally solve the problem, and when used simultaneously with a molluscicide, it can achieve double killing of eggs and molluscs, greatly reducing the density of molluscs and controlling the base number of molluscs.

[0030] In summary, this application includes at least one of the following beneficial technical effects: A Levisticum officinale water agent for destroying the eggs of Pomacea canaliculata and a preparation method disclosed in this application add 0.1%-2% of Levisticum officinale oil, so that the prepared Levisticum officinale water agent can destroy the eggs of Pomacea canaliculata, and has the advantages of good diffusibility, good ovicidal effect, low use cost, etc.; the Levisticum officinale water agent prepared in this application is a plant-derived ovicide, which can kill eggs and fundamentally solve the problem. Brief Description of the Drawings

[0031] Figure 1 Is the effect diagram before applying medicine to the eggs of Pomacea canaliculata; Figure 2 Is the control effect diagram of the Levisticum officinale water agent prepared by using Example 1 on the eggs of Pomacea canaliculata, where Figure 2 The left is the control effect diagram of the eggs of Pomacea canaliculata 1 week after applying medicine; Figure 2 The right is the control effect diagram of the eggs of Pomacea canaliculata without applying medicine after 1 week. Detailed Description of the Invention

[0032] The following refers to specific implementation examples to illustrate the present invention. Those skilled in the art can understand that these implementation examples are only used to illustrate the present invention and do not limit the scope of the present invention in any way.

[0033] The experimental methods in the following examples are all conventional methods unless otherwise specified. The medicinal material raw materials, reagent materials, etc. used in the following examples are all commercially available products unless otherwise specified.

[0034] The following further details the present application with reference to examples and comparative examples.

[0035] Preparation Example: The preparation method of Levisticum officinale oil, the steps are as follows: Crush 100 g of Levisticum officinale seeds into 60 meshes to obtain Levisticum officinale seed powder; Mix 100 g of Levisticum officinale seed powder with 210 mL of solvent ethanol, place it in an ultrasonic extractor and perform ultrasonic extraction for 30 minutes.

[0036] The conditions for ultrasonic extraction are as follows: power: 250 W; frequency: 40 kHz; temperature: 40 °C.

[0037] After the extraction is completed, the solid residue is removed by filtration, and the extract is concentrated (-0.06 MPa, 45 °C) to obtain a concentrated extract. The concentrated extract is dried to obtain 14.42 g of Levisticum oil.

[0038] Example 1: A Levisticum aqueous solution for destroying Pomacea canaliculata eggs. To prepare 100 g of the Levisticum aqueous solution, the raw materials are as follows by weight percentage: Levisticum oil 0.2%, isopropanol 18%, calcium dodecylbenzenesulfonate 10%, polyethylene glycol 4000 20%, distearoylethylenediamine 2%, and deionized water is added to make up to 100%.

[0039] The Levisticum oil is prepared by the method of Preparation Example.

[0040] The preparation method of the Levisticum aqueous solution is as follows: (1) According to the weight ratio of the formula; (2) Under normal temperature conditions, first add Levisticum oil to the modulation kettle, then add isopropanol and stir to dissolve; then add calcium dodecylbenzenesulfonate, polyethylene glycol 4000, water and distearoylethylenediamine, and stir and mix well for 2 hours to obtain the Levisticum aqueous solution.

[0041] Example 2: A Levisticum aqueous solution for destroying Pomacea canaliculata eggs. To prepare 100 g of the Levisticum aqueous solution, the raw materials are as follows by weight percentage: Levisticum oil 0.1%, isopropanol 10%, calcium dodecylbenzenesulfonate 5%, polyethylene glycol 4000 20%, distearoylethylenediamine 1%, and deionized water is added to make up to 100%.

[0042] The Levisticum oil is prepared by the method of Preparation Example.

[0043] The preparation method of the Levisticum aqueous solution is as follows: (1) According to the weight ratio of the formula; (2) Under normal temperature conditions, first add Levisticum oil to the modulation kettle, then add isopropanol and stir to dissolve; then add calcium dodecylbenzenesulfonate, polyethylene glycol 4000, water and distearoylethylenediamine, and stir and mix well for 2 hours to obtain the Levisticum aqueous solution.

[0044] Example 3: An Levisticum officinale water-based agent for destroying Pomacea canaliculata eggs. To prepare 100 g of the Levisticum officinale water-based agent, the raw materials are as follows by weight percentage: 1% of Levisticum officinale oil, 30% of isopropanol, 10% of calcium dodecylbenzenesulfonate, 30% of polyethylene glycol 4000, 2% of distearoylethylenediamine, and deionized water is added to make up 100%.

[0045] The Levisticum officinale oil is prepared by the method of Preparation Example.

[0046] The preparation method of the Levisticum officinale water-based agent is as follows: (1) According to the weight ratio of the formula; (2) Under normal temperature conditions, first add Levisticum officinale oil to the modulation kettle, then add isopropanol and stir to dissolve; then add calcium dodecylbenzenesulfonate, polyethylene glycol 4000, water and distearoylethylenediamine, and stir and mix well for 2 hours to obtain the Levisticum officinale water-based agent.

[0047] Example 4: An Levisticum officinale water-based agent for destroying Pomacea canaliculata eggs. To prepare 100 g of the Levisticum officinale water-based agent, the raw materials are as follows by weight percentage: 0.1% of Levisticum officinale oil, 15% of isopropanol, 8% of calcium dodecylbenzenesulfonate, 20% of polyethylene glycol 4000, 1% of distearoylethylenediamine, and deionized water is added to make up 100%.

[0048] The Levisticum officinale oil is prepared by the method of Preparation Example.

[0049] The preparation method of the Levisticum officinale water-based agent is as follows: (1) According to the weight ratio of the formula; (2) Under normal temperature conditions, first add Levisticum officinale oil to the modulation kettle, then add isopropanol and stir to dissolve; then add calcium dodecylbenzenesulfonate, polyethylene glycol 4000, water and distearoylethylenediamine, and stir and mix well for 2 hours to obtain the Levisticum officinale water-based agent.

[0050] Example 5: An Levisticum officinale water-based agent for destroying Pomacea canaliculata eggs. To prepare 100 g of the Levisticum officinale water-based agent, the raw materials are as follows by weight percentage: 0.5% of Levisticum officinale oil, 20% of isopropanol, 10% of calcium dodecylbenzenesulfonate, 25% of polyethylene glycol 4000, 2% of distearoylethylenediamine, and deionized water is added to make up 100%.

[0051] The Levisticum officinale oil is prepared by the method of Preparation Example.

[0052] The preparation method of the Levisticum officinale water-based agent is as follows: (1) According to the weight ratio of the formula; (2) Under normal temperature conditions, first add Levisticum officinale oil to the modulation kettle, and then add isopropanol and stir to dissolve; then add calcium dodecylbenzenesulfonate, polyethylene glycol 4000, water and distearoylethylenediamine, and fully stir and mix for 2 hours to obtain the Levisticum officinale aqueous solution.

[0053] Example 6: A Levisticum officinale aqueous solution for destroying the eggs of Pomacea canaliculata. Prepare 100 g of the Levisticum officinale aqueous solution. Each raw material is calculated by weight percentage: Levisticum officinale oil 0.1%, isopropanol 18%, calcium dodecylbenzenesulfonate 10%, polyethylene glycol 4000 20%, distearoylethylenediamine 2%, and deionized water is added to make up 100%.

[0054] The Levisticum officinale oil is prepared by the method of Preparation Example.

[0055] The preparation method of the Levisticum officinale aqueous solution is as follows: (1) According to the weight ratio of the formula; (2) Under normal temperature conditions, first add Levisticum officinale oil to the modulation kettle, and then add isopropanol and stir to dissolve; then add calcium dodecylbenzenesulfonate, polyethylene glycol 4000, water and distearoylethylenediamine, and fully stir and mix for 2 hours to obtain the Levisticum officinale aqueous solution.

[0056] Example 7: A Levisticum officinale aqueous solution for destroying the eggs of Pomacea canaliculata. Prepare 100 g of the Levisticum officinale aqueous solution. Each raw material is calculated by weight percentage: Levisticum officinale oil 1%, isopropanol 18%, calcium dodecylbenzenesulfonate 10%, polyethylene glycol 4000 20%, distearoylethylenediamine 2%, and deionized water is added to make up 100%.

[0057] The Levisticum officinale oil is prepared by the method of Preparation Example.

[0058] The preparation method of the Levisticum officinale aqueous solution is as follows: (1) According to the weight ratio of the formula; (2) Under normal temperature conditions, first add Levisticum officinale oil to the modulation kettle, and then add isopropanol and stir to dissolve; then add calcium dodecylbenzenesulfonate, polyethylene glycol 4000, water and distearoylethylenediamine, and fully stir and mix for 2 hours to obtain the Levisticum officinale aqueous solution.

[0059] Example 8: A Levisticum officinale aqueous solution for destroying the eggs of Pomacea canaliculata. Prepare 100 g of the Levisticum officinale aqueous solution. Each raw material is calculated by weight percentage: Levisticum officinale oil 0.5%, isopropanol 18%, calcium dodecylbenzenesulfonate 10%, polyethylene glycol 4000 20%, distearoylethylenediamine 2%, and deionized water is added to make up 100%.

[0060] The Levisticum officinale oil is prepared by the method of Preparation Example.

[0061] The preparation method of Levisticum officinale water-based agent is as follows: (1) According to the weight ratio of the formula; (2) Under normal temperature conditions, first add Levisticum officinale oil to the modulation kettle, then add isopropanol and stir to dissolve; then add calcium dodecylbenzenesulfonate, polyethylene glycol 4000, water and distearoylethylenediamine, and fully stir and mix for 2 hours to obtain Levisticum officinale water-based agent.

[0062] Comparative Example 1: A Levisticum officinale water-based agent for destroying the eggs of Pomacea canaliculata (i.e., an ovicide for destroying the eggs of Pomacea canaliculata), prepare 100 g of ovicide, and each raw material is calculated by weight percentage: isopropanol 18%, calcium dodecylbenzenesulfonate 10%, polyethylene glycol 4000 20%, distearoylethylenediamine 2%, and deionized water is added to make up to 100%.

[0063] The preparation method of the ovicide is as follows: (1) According to the weight ratio of the formula; (2) Under normal temperature conditions, add isopropanol to the modulation kettle and stir to dissolve; then add calcium dodecylbenzenesulfonate, polyethylene glycol 4000, water and distearoylethylenediamine, and fully stir and mix for 2 hours to obtain the ovicide.

[0064] Comparative Example 2: A Levisticum officinale water-based agent for destroying the eggs of Pomacea canaliculata, prepare 100 g of Levisticum officinale water-based agent, and each raw material is calculated by weight percentage: Levisticum officinale seed powder 0.2%, isopropanol 18%, calcium dodecylbenzenesulfonate 10%, polyethylene glycol 4000 20%, distearoylethylenediamine 2%, and deionized water is added to make up to 100%.

[0065] The preparation method of Levisticum officinale water-based agent is as follows: (1) According to the weight ratio of the formula; (2) Under normal temperature conditions, first add Levisticum officinale seed powder to the modulation kettle, then add isopropanol and stir to dissolve; then add calcium dodecylbenzenesulfonate, polyethylene glycol 4000, water and distearoylethylenediamine, and fully stir and mix for 2 hours to obtain Levisticum officinale water-based agent.

[0066] Comparative Example 3: A Levisticum officinale water-based agent for destroying the eggs of Pomacea canaliculata, prepare 100 g of Levisticum officinale water-based agent, and each raw material is calculated by weight percentage: Levisticum officinale oil 0.09%, isopropanol 18%, calcium dodecylbenzenesulfonate 10%, polyethylene glycol 4000 20%, distearoylethylenediamine 2%, and deionized water is added to make up to 100%.

[0067] Levisticum officinale oil is prepared by the method of Preparation Example.

[0068] The preparation method of Levisticum officinale water-based agent is as follows: (1) According to the weight ratio of the formula; (2) Under normal temperature conditions, first add Levisticum officinale oil to the modulation kettle, then add isopropanol and stir to dissolve; then add calcium dodecylbenzenesulfonate, polyethylene glycol 4000, water and distearoylethylenediamine, and fully stir and mix for 2 hours to obtain the Levisticum officinale aqueous solution.

[0069] Comparative Example 4: A Levisticum officinale aqueous solution for destroying the eggs of Pomacea canaliculata. Prepare 100 g of the Levisticum officinale aqueous solution. Each raw material is calculated by weight percentage: Levisticum officinale oil 1.1%, isopropanol 18%, calcium dodecylbenzenesulfonate 10%, polyethylene glycol 4000 20%, distearoylethylenediamine 2%, and deionized water is supplemented to 100%.

[0070] The Levisticum officinale oil is prepared by the method of Preparation Example.

[0071] The preparation method of the Levisticum officinale aqueous solution is as follows: (1) According to the weight ratio of the formula; (2) Under normal temperature conditions, first add Levisticum officinale oil to the modulation kettle, then add isopropanol and stir to dissolve; then add calcium dodecylbenzenesulfonate, polyethylene glycol 4000, water and distearoylethylenediamine, and fully stir and mix for 2 hours to obtain the Levisticum officinale aqueous solution.

[0072] Performance test 1: Laboratory hatching rate test, the steps are as follows: 1. Egg preparation: During the peak spawning period of Pomacea canaliculata, regularly collect the naturally laid egg masses; 2. Incubation condition setting: Place the egg masses in a constant temperature and humidity incubator at 27 ± 2 °C and relative humidity of 75%, and adopt a 12-hour light / 12-hour dark cycle; 3. Calculation method of hatching rate: Hatching rate = number of hatched Pomacea canaliculata eggs / total number of Pomacea canaliculata eggs × 100%.

[0073] 4. Experimental method: Preparation of the medicament: The dosage of the medicament corresponding to each Pomacea canaliculata egg mass is prepared as follows: Take 1 g of the Levisticum officinale aqueous solution (prepared in Examples 1-8 and Comparative Examples 1-4), add it to 9 ml of water and mix well to obtain the medicament.

[0074] Medication method: Spray the medicament on the Pomacea canaliculata egg masses to ensure uniform spraying. After spraying, incubate the egg masses, observe the development status of the Pomacea canaliculata eggs after 7-14 days, and calculate the hatching rate.

[0075] 5. Experimental Results: The detection results of the hatching rate of Pomacea canaliculata eggs after 14 days are shown in Table 1 below.

[0076] The effect diagram of Pomacea canaliculata eggs before applying the medicine is as Figure 1 shown; applying the Levisticum officinale water agent prepared in Example 1 to Pomacea canaliculata eggs, its control effect is as Figure 2 shown; according to Figure 2 it can be seen that Figure 2 On the left is the control effect diagram of Pomacea canaliculata eggs after applying the medicine for 1 week, indicating that after applying the medicine for 1 week, the Pomacea canaliculata eggs did not hatch, further demonstrating that the Levisticum officinale water agent prepared in this application can destroy the structure of Pomacea canaliculata eggs and prevent them from hatching, thereby achieving the killing of Pomacea canaliculata eggs; Figure 2 On the right is the control effect diagram of unmedicated Pomacea canaliculata eggs (blank group) after 1 week, indicating that the Pomacea canaliculata eggs were not medicated and hatched after 1 week.

[0077] Table 1 Detection Results of Hatching Rate Hatching rate (%) Example 1 0 Example 2 5 Example 3 4 Example 4 3 Example 5 2 Example 6 1 Example 7 3 Example 8 1 Comparative Example 1 100 Comparative Example 2 94 Comparative Example 3 76 Comparative Example 4 71 Blank group (without applying medicine) 100 Combined with Table 1, it can be seen that spraying the Levisticum officinale water agent (Examples 1-8) prepared by the method of this application on Pomacea canaliculata egg masses, after 14 days of applying the medicine, the hatching rate is 0-5%, indicating that the Levisticum officinale water agent prepared by the method of this application can destroy the structure of Pomacea canaliculata eggs and prevent them from hatching, thereby achieving the killing of Pomacea canaliculata eggs.

[0078] Combined with the data results of Example 1 and Comparative Example 1, it can be seen that the data result of Example 1 is better than that of Comparative Example 1, indicating that adding Levisticum officinale oil can kill Pomacea canaliculata eggs and prevent them from hatching.

[0079] Combined with the data results of Example 1 and Comparative Example 2, it can be seen that the data result of Example 1 is better than that of Comparative Example 2, indicating that the added Levisticum officinale oil is an extract of Levisticum officinale with high purity, and the high-purity Levisticum officinale oil effectively improves the killing performance of the water agent on Pomacea canaliculata eggs.

[0080] Combined with the data results of Example 1, Example 6, Example 7, Comparative Example 3, and Comparative Example 4, it can be seen that the addition amount of Levisticum officinale oil has a certain influence on the killing effect of Pomacea canaliculata eggs, and when the addition amount of Levisticum officinale oil is 0.1%-1% (by weight percentage), the effect is the best.

[0081] Combined with the data results of Example 1, Example 6, Example 7, and Example 8, it can be seen that when the addition amount of Levisticum officinale oil is 0.1%-0.5% (by weight percentage), the killing effect on Pomacea canaliculata eggs is the best.

[0082] Performance Detection 2: Field experiment, which is carried out according to the following steps during implementation: 1. Selection of test plot: From April to June, during the peak spawning period of Pomacea canaliculata, select waters such as paddy fields and water channels severely damaged by Pomacea canaliculata as the test sites.

[0083] 2. Experimental methods: Preparation of medicaments: Prepare the dosage of the medicament according to the number of Pomacea canaliculata egg masses in the area, specifically as follows: For the dosage of the medicament corresponding to each Pomacea canaliculata egg mass, the preparation steps are as follows: Take 1 g of Levisticum officinale water extract (prepared in Examples 1-8 and Comparative Examples 1-4), add it to 9 ml of water and mix evenly to obtain the medicament.

[0084] Application method of the medicament: Spray the medicament on the Pomacea canaliculata egg masses to ensure uniform spraying. After spraying, observe the development status of the Pomacea canaliculata eggs 7-14 days later and calculate the hatching rate.

[0085] 3. Experimental results: The detection results of the hatching rate of Pomacea canaliculata eggs after 14 days are shown in the following table.

[0086] Table 2 Detection results of hatching rate Combined with Table 1, it can be seen that spraying the Levisticum officinale water extract (Examples 1-8) prepared by the method of the present application on the Pomacea canaliculata egg masses, after 14 days of applying the medicine, the hatching rate is 0-5%, indicating that the Levisticum officinale water extract prepared by the method of the present application can damage the structure of Pomacea canaliculata eggs and make them unable to hatch, thereby achieving the killing of Pomacea canaliculata eggs.

[0087] Combined with the data results of Example 1 and Comparative Example 1, it can be seen that the data result of Example 1 is better than that of Comparative Example 1, indicating that the added Levisticum officinale oil can kill Pomacea canaliculata eggs and make them unable to hatch.

[0088] Combined with the data results of Example 1 and Comparative Example 2, it can be seen that the data result of Example 1 is better than that of Comparative Example 2, indicating that the added Levisticum officinale oil is the extract of Levisticum officinale with high purity, and the high-purity Levisticum officinale oil effectively improves the killing performance of the water extract on Pomacea canaliculata eggs.

[0089] Combined with the data results of Example 1, Example 6, Example 7, Comparative Example 3, and Comparative Example 4, it can be seen that the addition amount of Levisticum officinale oil has a certain influence on the killing effect of Pomacea canaliculata eggs, and when the addition amount of Levisticum officinale oil is 0.1%-1% (by weight percentage), the effect is the best.

[0090] Combined with the data results of Example 1, Example 6, Example 7, and Example 8, it can be seen that when the addition amount of Levisticum officinale oil is 0.1%-0.5% (by weight percentage), the killing effect on Pomacea canaliculata eggs is the best.

[0091] Performance test 3: The field experiment was carried out according to the following steps: 1. Test preparation (1) Test agent: 26% molluscicide suspension, manufacturer: Jiangsu Aijin Crop Science and Technology Group Co., Ltd.; The Angelica sinensis aqueous solution was prepared according to Examples 1-8 and Comparative Examples 1-4.

[0092] The preparation method of the medicine comprises the following steps: mixing 100 g of 26% molluscicide suspension, 100 g of angelica aqueous solution and 800 g of water to obtain the medicine; (2) Selection of test site: From April to June, during the peak spawning period of golden apple snails, we selected rice fields, canals and other water areas where golden apple snails caused more serious damage as test sites.

[0093] (3) Experimental site planning: The experimental site was divided into 4 plots, each plot generally covering an area of ​​50 square meters.

[0094] 2. Experimental methods: According to 1g / m 2 Use a sprayer to evenly spray the liquid medicine on the water surface, waterside plants and places where golden apple snails often appear in the test site. When applying the medicine, pay attention to the wind direction to prevent the liquid medicine from drifting to non-test areas.

[0095] After spraying, observe the development status of the golden apple snail eggs after 7-14 days and calculate the hatching rate; observe the growth status of the golden apple snails after 20-25 days and calculate the protective effect against the golden apple snails.

[0096] 3. Experimental results: Table 3 Experimental results As shown in Table 1, the Angelica sinensis aqueous solution (Examples 1-8) prepared by the method of the present application is used in combination with a molluscicide to kill Pomacea canaliculata and its eggs.

[0097] The present application discloses an Angelica sinensis aqueous solution for destroying golden apple snail eggs, which will provide a strong technical guarantee for the production of pollution-free vegetables, Chinese medicinal materials, fruits and green foods in my country. Through industrialization, it will significantly increase the output of plant-derived biological pesticides in my country, provide a continuous supply of biological pesticides for the development of pollution-free, green and organic foods, and safeguard the promotion of food production safety, agricultural product quality safety and agricultural ecological safety, and has a relatively broad market prospect.

Claims

1. A European Angelicae Sinensis aqueous solution for destroying Pomacea canaliculata eggs, characterized in that: Calculated by weight percentage, it includes 0.1%-1% of European Angelica extract, 10%-30% of solvent, 5%-10% of emulsifier, 20%-30% of auxiliary agent, 1%-2% of defoaming agent, and the balance is water.

2. The Angelica sinensis aqueous solution for destroying Pomacea canaliculata eggs according to claim 1, characterized in that: Calculated by weight percentage, it includes 0.1%-0.5% of European Angelica extract, 15%-20% of solvent, 8%-10% of emulsifier, 20%-25% of auxiliary agent, 1%-2% of defoaming agent, and the balance is water.

3. The Angelica sinensis aqueous solution for destroying Pomacea canaliculata eggs according to claim 1 or 2, characterized in that: The solvent includes at least one of isobutyl alcohol, isopropyl alcohol, dimethyl sulfoxide, dimethylformamide and ether.

4. The Angelica sinensis aqueous solution for destroying Pomacea canaliculata eggs according to claim 1 or 2, characterized in that: The emulsifier includes at least one of calcium dodecylbenzene sulfonate, fatty alcohol polyoxyethylene ether, castor oil polyoxyethylene ether, Ethylan NS-500LQ nonionic hydroxy polyethylene oxide block copolymer, and sodium di-sec-octyl maleate sulfonate.

5. The Angelica sinensis aqueous solution for destroying Pomacea canaliculata eggs according to claim 1 or 2, characterized in that: The auxiliary agent includes at least one of phospholipids, trisiloxane, octamethylcyclotetrasiloxane and polyethylene glycol.

6. The Angelica sinensis aqueous solution for destroying Pomacea canaliculata eggs according to claim 1 or 2, characterized in that: The defoaming agent includes at least one of isooctyl alcohol, polyol fatty acid ester, distearyl ethylenediamine and halogenated hydrocarbon.

7. The Angelica sinensis aqueous solution for destroying Pomacea canaliculata eggs according to claim 1 or 2, characterized in that: The Angelica sinensis extract is Angelica sinensis oil.

8. The Angelica sinensis aqueous solution for destroying Pomacea canaliculata eggs according to claim 7, characterized in that: The preparation method of the Angelica dahurica oil is as follows: Grinding the Angelica sinensis seeds to obtain Angelica sinensis seed powder; The angelica seed powder is mixed with a solvent, and ultrasonic extraction, filtration and concentration are performed to obtain the angelica oil.

9. The Angelica sinensis aqueous solution for destroying Pomacea canaliculata eggs according to claim 8, characterized in that: The mass volume ratio of the Angelica sinensis seed powder to the solvent is (1-5) g: (2-6) ml.

10. A method for preparing an angelica sinensis aqueous solution for destroying Pomacea canaliculata eggs, characterized in that: The steps of the preparation method are as follows: Weigh each raw material according to the formula; The angelica extract and the solvent are mixed, and then an emulsifier, an auxiliary agent, water and a defoaming agent are added and mixed to obtain the angelica aqueous solution.

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