Preparation method and application of protein polypeptide graft for enhancing immunity

Abstract

The invention discloses a preparation method and application of a protein polypeptide graft capable of enhancing immunity, and relates to the technical field of nutriment preparation, a stirrer is applied in the preparation method of the protein polypeptide graft capable of enhancing immunity, the stirrer comprises an operation tank, and a first stirring chamber and a second stirring chamber are symmetrically formed in the operation tank; and a first stirring shaft is arranged in the first stirring chamber in a penetrating mode, the top of the first stirring shaft is connected with a first transmission disc, and the top of the first transmission disc is connected with a speed adjusting motor. According to the preparation method and application of the protein polypeptide graft capable of enhancing immunity, the anti-virus and anti-infection capability of a human body can be improved, so that the defense function of the human body is improved, a certain protein protection function is achieved, the polypeptide polysaccharide graft is formed through reaction, the bad taste of protein polypeptide is improved, meanwhile, the moisture absorption problem of polysaccharide is solved, and the immunity of the human body is enhanced. Not only is the taste ensured, but also the shelf life of the product is prolonged.

Description

Technical Field

[0001] The present invention relates to the technical field of nutritional product preparation, and particularly to a preparation method and application of an immune-enhancing protein polypeptide graft. Background Art

[0002] When a living body has normal immunity, it can resist external invasions and repair its own cells to maintain health. However, when the body's immune level decreases due to reasons such as diseases, malnutrition, or changes in the external environment, it will cause harm to the body or aggravate the symptoms. To get rid of stubborn chronic diseases, the key is to mobilize one's own immunity, make full use of the regulatory role of the immune system, establish a strong firewall of one's own, establish a natural disease-resistant barrier, and prevent diseases from invading. Milk protein, egg white protein, and polysaccharides are all beneficial substances that can improve human immunity. The polypeptides obtained by enzymatic hydrolysis of proteins can be quickly absorbed and have a high absorption rate. However, the protein after forming polypeptides has a bitter and astringent taste, making it difficult to be accepted; soluble polysaccharides have a light sweet taste and are easily accepted, but soluble polysaccharides are prone to moisture absorption and agglomeration during storage, resulting in a shortened shelf life of such substances during application.

[0003] Therefore, in view of this, in order to study and improve the existing structure and deficiencies, a preparation method and application of an immune-enhancing protein polypeptide graft are proposed. Summary of the Invention

[0004] In view of the deficiencies of the prior art, the present invention provides a preparation method and application of an immune-enhancing protein polypeptide graft, and solves the problems raised in the above background art.

[0005] To achieve the above objectives, the present invention is realized through the following technical solutions: A preparation method of an immune-enhancing protein polypeptide graft, the preparation method of the immune-enhancing protein polypeptide graft includes the following steps:

[0006] Step 1: Add purified water 1.5 times the formula amount of milk protein and egg white protein into the first stirring chamber, set the temperature to 40 °C, add the formula amount of milk protein and egg white protein in sequence, stir to dissolve them, then raise the temperature to 60 °C, add sodium citrate solution to adjust the PH to 9.5, and then add 1% of the protein formula amount of alkaline protease 100,000 U / g, keep warm and continuously stir and react for 1 h to obtain a protein hydrolysate;

[0007] Step 2: Add purified water 0.5 times the formula amount of polysaccharide into the second stirring chamber, set the temperature to 40 °C, stir to dissolve it fully, then add citric acid solution to adjust the PH to 3.0, raise the temperature to 75 °C, and continuously stir for 1 h to obtain a polysaccharide solution;

[0008] Step 3: The electric telescopic rod extends, enabling the pressing tube roller to press on the surface of the stressed hose and rotate synchronously with the second stirring shaft. Thus, the polysaccharide solution inside the second stirring chamber is continuously injected into the slope box. The slope box rotates with the first stirring shaft and evenly scatters the polysaccharide solution into the protein hydrolysate. Sodium citrate solution is added to the first stirring chamber under stirring to adjust the pH to 9.5, and the temperature is continuously raised to 85 °C and continuously stirred for reaction for 1.5 h to obtain the grafted reactant;

[0009] Step 4: The grafted reactant is cooled to 40 °C, and citric acid solution is added under stirring to adjust the pH to 6.5. Then it is pumped into a high-speed centrifugal spray dryer, the inlet air temperature is adjusted to 155 °C, and spray drying is carried out. After drying, it is sieved through a 20-mesh sieve to obtain the protein-polypeptide-polysaccharide graft.

[0010] Furthermore, the preparation method of the immunopotentiating protein-polypeptide graft uses a stirrer, which includes an operation tank. Inside the operation tank, a first stirring chamber and a second stirring chamber are symmetrically arranged. A first stirring shaft penetrates through the inside of the first stirring chamber. The top of the first stirring shaft is connected with a first transmission disc, and the top of the first transmission disc is connected with a speed-regulating motor.

[0011] Furthermore, a second stirring shaft penetrates through the inside of the second stirring chamber. The top of the second stirring shaft is connected with a gearbox, the top of the gearbox is connected with a second transmission disc, and a transmission belt is sleeved on the sides of the second transmission disc and the first transmission disc.

[0012] Furthermore, an upper part of the inner wall of the second stirring chamber is provided with a slide rail groove, and a peristaltic infusion assembly is arranged inside the slide rail groove.

[0013] Furthermore, the peristaltic infusion assembly includes a stressed hose, a feed pipe, and a discharge pipe. One end of the stressed hose is connected with the feed pipe, and the other end of the stressed hose is connected with the discharge pipe.

[0014] Furthermore, the peristaltic infusion assembly further includes a battery box, an electric telescopic rod, and a pressing tube roller. An upper part of the outer wall of the second stirring shaft is fixed with the battery box, and electric telescopic rods are arranged on both sides of the battery box. The end of the electric telescopic rod is rotatably connected with the pressing tube roller.

[0015] Furthermore, a uniform liquid distribution assembly is fixed on the upper part of the outer wall of the first stirring shaft. The uniform liquid distribution assembly includes a support frame, a slope box, liquid distribution holes, and a rotating ring cover. The outer end of the support frame is fixed with the slope box, and liquid distribution holes are evenly arranged at the bottom of the slope box. The top of the slope box is rotatably connected with the rotating ring cover. One end of the discharge pipe penetrates through the surface of the rotating ring cover, and the discharge pipe has an inverted U-shaped structure.

[0016] Furthermore, the protein-peptide graft in the preparation method of the immune-enhancing protein-peptide graft comprises the following raw materials in parts by weight:

[0017] The milk protein contains casein, 60-70 parts of albumin, 5-10 parts of egg white protein, and 20-30 parts of polysaccharide;

[0018] The polysaccharide formula includes 5 parts of isomaltooligosaccharide, 4 parts of fructooligosaccharide and 1 part of stachyose.

[0019] Furthermore, in the step 1, the output speed of the speed regulating motor, i.e., the speed of the first stirring shaft, is 150 r / min;

[0020] In step 2, the rotation speed of the second stirring shaft is 150 r / min;

[0021] In step three, the stirring speed of the first stirring shaft is 120 r / min, while the speed of the second stirring shaft remains unchanged through the gearbox.

[0022] Furthermore, the method for preparing the protein polypeptide graft for enhancing immunity is applied in the technical field of nutrient preparation.

[0023] The present invention provides a method for preparing an immune-enhancing protein polypeptide graft and its application, which has the following beneficial effects:

[0024] 1. The preparation method and application of the immune-enhancing protein polypeptide graft can improve the human body's anti-virus and anti-infection capabilities, thereby improving the body's defense function, and also has a certain protein protection function. By reacting to form a polypeptide polysaccharide graft, not only the bad taste of the protein polypeptide is improved, but also the moisture absorption problem of the polysaccharide is improved, which not only ensures the taste but also extends the shelf life of the product.

[0025] 2. The preparation method and application of the protein polypeptide graft for enhancing immunity, through a single speed-regulating motor and a gearbox, can simultaneously enable the first stirring shaft and the second stirring shaft to complete the preparation and transportation of two solutions at different rotation speeds, wherein when the electric telescopic rod is extended to make the tube pressing roller rotate, the force-bearing hose is continuously squeezed, thereby allowing the polysaccharide solution to automatically flow into the first stirring chamber and mix with the protein hydrolyzate, and the bottom of the slope box is arranged in a slope, and under the action of its rotating centrifugal force, the polysaccharide solution passes through the liquid separation hole and is evenly sprinkled from above the protein hydrolyzate, thereby improving the mixing effect and speed of the polysaccharide solution and the protein hydrolyzate. BRIEF DESCRIPTION OF THE DRAWINGS

[0026] Figure 1 It is a schematic diagram of the internal structure of the engine speed sensor working tank of the present invention;

[0027] Figure 2 It is a schematic diagram of the appearance structure of the top surface of the working tank of the present invention;

[0028] Figure 3 This is a schematic diagram of the appearance structure of the bottom of the working tank of the present invention;

[0029] Figure 4 This is a schematic diagram of the structure of the second stirring shaft of the present invention;

[0030] Figure 5 This is a schematic diagram of the structure of the first stirring shaft of the present invention;

[0031] Figure 6 This is a schematic diagram of the internal structure of the slope box of the present invention;

[0032] Figure 7 This is a schematic diagram of the test results of the connection system of the present invention.

[0033] In the figure: 1. Working tank; 2. First stirring chamber; 3. Second stirring chamber; 4. First stirring shaft; 5. First transmission disc; 6. Speed-regulating motor; 7. Second stirring shaft; 8. Gearbox; 9. Second transmission disc; 10. Transmission belt; 11. Slide rail groove; 12. Peristaltic infusion assembly; 1201. Force-bearing hose; 1202. Feed pipe; 1203. Discharge pipe; 1204. Battery box; 1205. Electric telescopic rod; 1206. Pipe-pressing roller; 13. Uniform liquid distribution assembly; 1301. Support frame; 1302. Slope box; 1303. Liquid distribution hole; 1304. Rotating ring cover. Specific embodiments

[0034] The following further describes in detail the embodiments of the present invention in conjunction with the drawings and examples. The following examples are used to illustrate the present invention, but cannot be used to limit the scope of the present invention.

[0035] As Figures 1-7 shown, the present invention provides a technical solution: A preparation method of an immunopotentiating protein polypeptide graft, wherein the protein polypeptide graft comprises the following raw materials in parts by weight:

[0036] Milk protein comprises casein, albumin 60 - 70 parts, egg white protein 5 - 10 parts, and polysaccharide 20 - 30 parts;

[0037] Among them, the formula of the polysaccharide comprises isomaltooligosaccharide 5 parts, fructooligosaccharide 4 parts, and stachyose 1 part;

[0038] The preparation method of the immunopotentiating protein polypeptide graft comprises the following steps:

[0039] Step 1: Add purified water which is 1.5 times the formula amount of milk protein and egg white protein into the first stirring chamber 2, set the temperature to 40°C, sequentially add the formula amount of milk protein and egg white protein, stir to dissolve them, then raise the temperature to 60°C, add sodium citrate solution to adjust the pH to 9.5, and then add alkaline protease 100,000 U / g in an amount of 1% of the protein formula, and keep warm and continuously stir for reaction for 1 h to obtain a protein hydrolysate;

[0040] Step 2: Add purified water which is 0.5 times the formula amount of polysaccharide into the second stirring chamber 3, set the temperature to 40°C, stir to dissolve it fully, then add citric acid solution to adjust the pH to 3.0, raise the temperature to 75°C and continuously stir for 1 h to obtain a polysaccharide solution;

[0041] Step 3: The electric telescopic rod 1205 extends so that the pressing tube roller 1206 presses on the surface of the stress hose 1201 and rotates synchronously with the second stirring shaft 7, thereby continuously injecting the polysaccharide solution inside the second stirring chamber 3 into the slope box 1302. The slope box 1302 rotates with the first stirring shaft 4 and evenly scatters the polysaccharide solution into the protein hydrolysate. Add sodium citrate solution in the first stirring chamber 2 under stirring to adjust the pH to 9.5, continuously raise the temperature to 85°C and continuously stir for reaction for 1.5 h to obtain a graft reaction product;

[0042] Step 4: Cool the graft reaction product to 40°C, add citric acid solution in the stirring state to adjust the pH to 6.5, then pump it into a high-speed centrifugal spray dryer, adjust the inlet air temperature to 155°C, and carry out spray drying. After drying, pass through a 20-mesh sieve to obtain a protein-polypeptide-polysaccharide graft;

[0043] In Step 1, the output rotation speed of the speed-regulating motor 6, that is, the rotation speed of the first stirring shaft 4, is 150 r / min;

[0044] In Step 2, the rotation speed of the second stirring shaft 7 is 150 r / min;

[0045] In Step 3, the stirring rotation speed of the first stirring shaft 4 is 120 r / min, and the rotation speed of the second stirring shaft 7 remains unchanged through the gearbox 8;

[0046] The protein-polypeptide-polysaccharide graft can be made into an oral preparation. The oral preparation can be powder, tablet, granule or drink, and flavoring sugars, sugar alcohols, fruit powders, fruit grains, milk or cheese and other flavoring agents or nutrients such as probiotics can also be added according to the taste requirements;

[0047] Example 1:

[0048] An immune-enhancing milk protein polypeptide graft, comprising the following raw materials in parts by weight:

[0049] 60 parts of milk protein (mainly casein and albumin), 10 parts of egg white protein, and 30 parts of polysaccharide (including 5 parts of isomaltooligosaccharide, 4 parts of fructooligosaccharide, and 1 part of stachyose).

[0050] The preparation method includes the following steps:

[0051] Step 1: Protein hydrolysis:

[0052] Add purified water 1.5 times the formula amount of milk protein and egg white protein into the first stirring chamber 2, set the temperature to 40 °C, and the magnetic stirring speed to 150 r / min. When the temperature rises to 40 °C, add the formula amount of milk protein and egg white protein in sequence. After stirring to dissolve, raise the temperature to 60 °C, add an appropriate amount of sodium citrate solution, adjust the pH to 9.5, then add 1% of the formula amount of milk protein of alkaline protease (100,000 U / g), and keep stirring and reacting for 1 h at a constant temperature;

[0053] Step 2: Polysaccharide dissolution:

[0054] Add purified water 0.5 times the formula amount of polysaccharide into the second stirring chamber 3, set the temperature to 40 °C, and the magnetic stirring speed to 150 r / min. When the temperature rises to 40 °C, add the formula amount of polysaccharide. After stirring to dissolve it completely, add an appropriate amount of citric acid solution, adjust the pH to 3.0, raise the temperature to 75 °C, and keep stirring for 1 h;

[0055] Step 3: Grafting reaction:

[0056] Transfer the polysaccharide solution after stirring for 1 h in Step 2 into the protein hydrolysate after reacting for 1 h in Step 1. Add an appropriate amount of sodium citrate solution to adjust the pH to 9.5 under stirring. Set the temperature to 85 °C, the stirring speed to 120 r / min, continuously raise the temperature to 85 °C, and keep stirring and reacting for 1.5 h in this temperature range;

[0057] Step 4: Drying:

[0058] Cool the grafting reactant in Step 3 to 40 °C, add an appropriate amount of citric acid solution to adjust the pH to 6.5 under stirring, and then pump the reactant into a high-speed centrifugal spray dryer (model: MDR-50) through a peristaltic pump (model: KZ15, adjust the speed to 15 rpm). Adjust the inlet air temperature to 155 °C for spray drying. After drying, pass through a 20-mesh sieve to obtain the milk protein polypeptide polysaccharide graft;

[0059] Example 2:

[0060] An immune-enhancing milk protein polypeptide polysaccharide reactant, including the following raw materials in parts by weight:

[0061] 80 parts of milk protein (mainly casein and albumin), 5 parts of egg white protein, 15 parts of polysaccharide (including 5 parts of isomaltooligosaccharide, 4 parts of fructooligosaccharide, and 1 part of stachyose);

[0062] The preparation method includes the following steps:

[0063] Step 1: Protein hydrolysis:

[0064] Add purified water 1.5 times the formula amount of milk protein and egg white protein into the first stirring chamber 2, set the temperature to 40°C, and the magnetic stirring speed to 150 r / min. When the temperature rises to 40°C, add the formula amount of milk protein and egg white protein in sequence. After stirring to dissolve, raise the temperature to 50°C, add an appropriate amount of sodium citrate solution, adjust the pH to 9.5, then add 1% of the protein formula amount of alkaline protease (100,000 U / g), and keep stirring and reacting for 1 h.

[0065] Step 2: Polysaccharide dissolution:

[0066] Add purified water 0.5 times the formula amount of polysaccharide into the second stirring chamber 3, set the temperature to 40°C, and the magnetic stirring speed to 150 r / min. When the temperature rises to 40°C, add the formula amount of polysaccharide. After stirring to fully dissolve, add an appropriate amount of citric acid solution, adjust the pH to 3.0, raise the temperature to 75°C, and keep stirring for 1 h.

[0067] Step 3: Grafting reaction:

[0068] Transfer the polysaccharide solution after stirring for 1 h in Step 2 into the protein hydrolysate after reacting for 1 h in Step 1. Add an appropriate amount of sodium citrate solution to adjust the pH to 9.5 under stirring, set the temperature to 85°C, and the stirring speed to 150 r / min. Keep heating to 85°C and keep stirring and reacting for 1.5 h in this temperature range.

[0069] Step 4: Drying:

[0070] Cool the grafted reactant in Step 3 to 40°C, add an appropriate amount of citric acid solution to adjust the pH to 6.5 under stirring, then pump the reactant into a high-speed centrifugal spray dryer (model: MDR-50) through a peristaltic pump (model: KZ15, adjust the speed to 15 rpm), adjust the inlet air temperature to 155°C, and perform spray drying. After drying, pass through a 20-mesh sieve to obtain the protein-polypeptide-polysaccharide graft;

[0071] The difference between Example 2 and Example 1 lies in the content of protein and polysaccharide components. The solubilities of the reactants obtained in the two examples are close, and there are slight differences in taste. The sweetness of Example 1 is slightly greater than that of Example 2;

[0072] Perform grafting degree tests on the reactants of Example 1 and Example 2 respectively. The specific test method is as follows:

[0073] Weigh 50 mg of the protein sample and place it in a 100 mL volumetric flask. Add an appropriate amount of distilled water and shake to dissolve. Then, make up the volume to 100 mL with distilled water. After shaking well, pour out an appropriate amount into a centrifuge tube and centrifuge at 6000 r·min-1 for 20 min. Measure 200 μL of the supernatant and place it in a stoppered test tube. Add 4 mL of distilled water, shake well, and measure the absorbance at a wavelength of 340 nm. The blank control is distilled water to obtain A0. Then, measure another 200 μL of the supernatant after centrifugation and place it in a stoppered test tube. Add 4 mL of o-phthalaldehyde (OPA) solution, shake well, and place it in a water bath. React for 2 min at 35 °C in the dark. Measure the absorbance at a wavelength of 340 nm. The blank control is distilled water to obtain A t , based on the following formula:

[0074]

[0075] A0 is the absorbance value of the unreacted solution in L·(g·cm)-1; A t is the absorbance of the solution after the reaction in L·(g·cm)-1.

[0076] Preparation of the above sodium dodecyl sulfate (SDS) solution (0.2 mg / mL): Weigh 20 mg of SDS and place it in a 100 mL volumetric flask. Add an appropriate amount of distilled water to dissolve and then make up the volume to 100 mL with distilled water. Shake well and set aside for use;

[0077] Preparation of the above sodium tetraborate solution (0.1 mol / L, pH 9.5): Weigh 3.81 g of sodium tetraborate and place it in a 100 mL volumetric flask. Add an appropriate amount of distilled water to dissolve and then make up the volume to 100 mL with distilled water. Shake well, measure the pH, and set aside for use;

[0078] Preparation of the above o-phthalaldehyde (OPA) solution: Weigh 40 mg of o-phthalaldehyde and place it in a 50 mL volumetric flask. Add 1 mL of distilled water to dissolve, then add 2.5 mL of the sodium dodecyl sulfate (SDS) solution (0.2 mg / mL) prepared in 1.1, then add the sodium tetraborate solution (0.1 mol / L, pH 9.5) prepared in 1.2, then add 0.1 mL of β-mercaptoethanol, and finally make up the volume to 50 mL with distilled water. Shake well and set aside for use;

[0079] The test results of the grafting degree are shown in Figure 7 ;

[0080] It is concluded from the above tests that the grafting degree of the protein polypeptide polysaccharide obtained by this formula and process is relatively high. After the polysaccharide ratio is increased, the grafting degree will increase to a certain extent. Considering the different demands of the human body for high-quality protein and polysaccharide, the ratio of protein to polysaccharide is determined to be 3 - 4:2 - 1;

[0081] The zebrafish tests were conducted on the reactants of the two embodiments respectively. According to the evaluation criteria, the compositions of the above embodiment groups can promote the transformation of lymphocytes, enhance the activities of T cells and NK cells, repair damaged cells, improve the body's anti-viral and anti-infection abilities, and thus improve the body's defense function;

[0082] The above embodiments only represent one implementation mode of the present invention, which proves that the above solutions can all improve the body's anti-viral and anti-infection abilities, thereby improving the body's defense function, and also have a certain protein protection function.

[0083] Based on the above description, the present invention can improve the body's anti-viral and anti-infection abilities, thereby improving the body's defense function, and also has a certain protein protection function. Moreover, through the reaction to form a polypeptide-polysaccharide graft, it not only improves the poor taste of protein polypeptides, but also improves the moisture absorption problem of polysaccharides, not only ensuring the taste but also extending the shelf life of the product.

[0084] As Figures 1-6 shown, a stirrer is used in the preparation method of the immune-enhancing protein polypeptide graft. The stirrer includes an operation tank 1. First stirring chambers 2 and second stirring chambers 3 are symmetrically arranged inside the operation tank 1. A first stirring shaft 4 penetrates through the inside of the first stirring chamber 2. A first transmission disk 5 is connected to the top of the first stirring shaft 4. A speed-regulating motor 6 is connected to the top of the first transmission disk 4. A second stirring shaft 7 penetrates through the inside of the second stirring chamber 3. A gearbox 8 is connected to the top of the second stirring shaft 7. A second transmission disk 9 is connected to the top of the gearbox 8. A transmission belt 10 is sleeved on the side surfaces of the second transmission disk 9 and the first transmission disk 4. An upper part of the inner wall of the second stirring chamber 3 is provided with a slide rail groove 11. A peristaltic infusion assembly 12 is arranged inside the slide rail groove 11. The peristaltic infusion assembly 12 includes a stress hose 1201, a feed pipe 1202 and a discharge pipe 1203. One end of the stress hose 1201 is connected to the feed pipe 1202, and the other end of the stress hose 1201 is connected to the discharge pipe 1203. The peristaltic infusion assembly 12 further includes a battery box 1204, an electric telescopic rod 1205 and a pressure tube roller 1206. A battery box 1204 is fixed to the upper part of the outer wall of the second stirring shaft 7. Electric telescopic rods 1205 are arranged on both sides of the battery box 1204. The end of the electric telescopic rod 1205 is rotatably connected to the pressure tube roller 1206. A uniform liquid distribution assembly 13 is fixed to the upper part of the outer wall of the first stirring shaft 4. The uniform liquid distribution assembly 13 includes a support frame 1301, a slope box 1302, liquid distribution holes 1303 and a rotating ring cover 1304. The outer end of the support frame 1301 is fixed to the slope box 1302. Liquid distribution holes 1303 are evenly opened at the bottom of the slope box 1302. A rotating ring cover 1304 is rotatably connected to the top of the slope box 1302. One end of the discharge pipe 1203 penetrates through the surface of the rotating ring cover 1304, and the discharge pipe 1203 is in an inverted U-shaped structure;

[0085] The specific operation is as follows. The protein hydrolyzate solution and the polysaccharide solution are respectively prepared simultaneously inside the first stirring chamber 2 and the second stirring chamber 3. Among them, the speed-regulating motor 6 drives the first stirring shaft 4 to stir the various ingredients inside the first stirring chamber 2 at a speed of 150 r / min. Through the first transmission disk 5 and the transmission belt 10, the second transmission disk 9 is synchronously driven to rotate, and through the gearbox 8, the second stirring shaft 7 stirs the various ingredients inside the second stirring chamber 3 at a speed of 150 r / min. Thus, the protein hydrolyzate solution and the polysaccharide solution are respectively formed. Heating mechanisms are provided inside both the first stirring chamber 2 and the second stirring chamber 3 to provide the temperatures required for the preparation of the two solutions.

[0086] During the preparation of the polysaccharide solution, the electric telescopic rod 1205 is in a contracted state. When the polysaccharide solution is prepared and needs to be transported from the second stirring chamber 3 to the inside of the first stirring chamber 2 to be mixed with the protein hydrolyzate solution, the battery box 1204 supplies power to the electric telescopic rod 1205 to make it extend, so that the pressure tube roller 1206 extends into the slide rail groove 11. The battery box 1204 rotates synchronously with the second stirring shaft 7, making the pressure tube roller 1206 perform a rotational motion to continuously squeeze the stress hose 1201, so that the polysaccharide solution flows successively along the feed pipe 1202, the stress hose 1201, and the discharge pipe 1203, and finally the polysaccharide solution is injected into the slope box 1302.

[0087] The slope box 1302 rotates synchronously with the first stirring shaft 4. The top of the slope box 1302 is rotatably connected to the rotating ring cover 1304. One end of the discharge pipe 1203 penetrates the surface of the rotating ring cover 1304 and enters the inside of the slope box 1302. Therefore, the rotating ring cover 1304 remains stationary and does not affect the rotation of the slope box 1302. At the same time, the rotating ring cover 1304 can prevent the polysaccharide solution from overflowing from the top. The bottom of the slope box 1302 is sloped. Under the action of the rotational centrifugal force, the polysaccharide solution passes through the liquid separation holes 1303 and evenly spills from above the protein hydrolyzate solution, and then the first stirring shaft 4 rotates to stir and mix the polysaccharide solution and the protein hydrolyzate solution.

[0088] Based on the above description, the present invention can simultaneously make the first stirring shaft 4 and the second stirring shaft 7 complete the preparation and transportation of the two solutions at different speeds through a single speed-regulating motor 6 in cooperation with a gearbox 8. Among them, when the electric telescopic rod 1205 extends to make the pressure tube roller 1206 perform a rotational motion, the stress hose 1201 is continuously squeezed, so that the polysaccharide solution automatically flows into the first stirring chamber 2 to be mixed with the protein hydrolyzate solution. The bottom of the slope box 1302 is sloped. Under the action of its rotational centrifugal force, the polysaccharide solution passes through the liquid separation holes 1303 and evenly spills from above the protein hydrolyzate solution, thereby improving the mixing effect and speed of the polysaccharide solution and the protein hydrolyzate solution.

[0089] The preparation method of the protein polypeptide graft for enhancing immunity is applied to the technical field of nutritional product preparation.

[0090] The embodiments of the present invention are given for purposes of illustration and description, and are not exhaustive or limit the invention to the disclosed form. Many modifications and variations are obvious to those of ordinary skill in the art. The embodiments are chosen and described in order to best explain the principles of the invention and its practical application, and to enable those of ordinary skill in the art to understand the invention and design various embodiments with various modifications suitable for a particular purpose.

Claims

1. A method for preparing a protein polypeptide graft for enhancing immunity, characterized in that: The method for preparing the protein polypeptide graft for enhancing immunity comprises the following steps: Step 1: Add 1.5 times the amount of purified water of the formula of milk protein and egg white protein into the first stirring chamber, set the temperature to 40°C, add the formula amount of milk protein and egg white protein in turn, stir to dissolve, then raise the temperature to 60°C, add sodium citrate solution to adjust the pH to 9.5, then add 1% of the protein formula amount of alkaline protease 100,000 U / g, keep warm and continue stirring for 1 hour to obtain protein hydrolyzate; Step 2: Add 0.5 times the amount of purified water in the second stirring chamber, set the temperature to 40°C, stir to fully dissolve, then add citric acid solution to adjust the pH to 3.0, raise the temperature to 75°C and continue stirring for 1 hour to obtain a polysaccharide solution; Step 3: The electric telescopic rod is extended so that the tube pressing roller can be pressed on the surface of the stressed hose and rotate synchronously with the second stirring shaft, thereby continuously injecting the polysaccharide solution in the second stirring chamber into the slope box, and the slope box rotates with the first stirring shaft to evenly sprinkle the polysaccharide solution into the protein hydrolyzate, and sodium citrate solution is added to the first stirring chamber under stirring to adjust the pH to 9.5, and the temperature is continuously raised to 85°C and stirred for 1.5 hours to obtain the grafted reactant; Step 4: The grafted reactants are cooled to 40°C, citric acid solution is added under stirring to adjust the pH to 6.5, and then pumped into a high-speed centrifugal spray dryer, the inlet air temperature is adjusted to 155°C, and spray dried. After drying, the reactants are sieved through a 20-mesh sieve to obtain a protein, polypeptide, and polysaccharide grafted product.

2. The method for preparing a protein polypeptide graft for enhancing immunity according to claim 1, characterized in that: The method for preparing the protein polypeptide graft for enhancing immunity uses an agitator, which comprises an operating tank (1), wherein a first stirring chamber (2) and a second stirring chamber (3) are symmetrically provided inside the operating tank (1), and a first stirring shaft (4) is passed through the first stirring chamber (2), a first transmission disc (5) is connected to the top of the first stirring shaft (4), and a speed regulating motor (6) is connected to the top of the first transmission disc (4).

3. The method for preparing a protein polypeptide graft for enhancing immunity according to claim 2, characterized in that: A second stirring shaft (7) is inserted into the interior of the second stirring chamber (3), and a gearbox (8) is connected to the top of the second stirring shaft (7), and a second transmission disc (9) is connected to the top of the gearbox (8), and a transmission belt (10) is sleeved on the sides of the second transmission disc (9) and the first transmission disc (4).

4. The method for preparing a protein polypeptide graft for enhancing immunity according to claim 3, characterized in that: A slide rail groove (11) is provided on the upper portion of the inner wall of the second stirring chamber (3), and a peristaltic infusion assembly (12) is provided inside the slide rail groove (11).

5. The method for preparing a protein polypeptide graft for enhancing immunity according to claim 4, characterized in that: The peristaltic infusion assembly (12) comprises a force-bearing hose (1201), a feed pipe (1202) and a discharge pipe (1203), wherein one end of the force-bearing hose (1201) is connected to the feed pipe (1202), and the other end of the force-bearing hose (1201) is connected to the discharge pipe (1203).

6. The method for preparing a protein polypeptide graft for enhancing immunity according to claim 5, characterized in that: The peristaltic infusion assembly (12) further comprises a battery box (1204), an electric telescopic rod (1205) and a tube pressing roller (1206); the battery box (1204) is fixed to the upper portion of the outer wall of the second stirring shaft (7), and the electric telescopic rod (1205) is arranged on both sides of the battery box (1204); the end of the electric telescopic rod (1205) is rotatably connected to the tube pressing roller (1206).

7. The method for preparing a protein polypeptide graft for enhancing immunity according to claim 6, characterized in that: A uniform liquid separation component (13) is fixed to the upper part of the outer wall of the first stirring shaft (4), and the uniform liquid separation component (13) comprises a support frame (1301), a slope box (1302), liquid separation holes (1303) and a rotating ring cover (1304). The outer end of the support frame (1301) is fixed with the slope box (1302), and the bottom of the slope box (1302) is uniformly provided with liquid separation holes (1303). The top of the slope box (1302) is rotatably connected to the rotating ring cover (1304), and one end of the discharge pipe (1203) passes through the surface of the rotating ring cover (1304), and the discharge pipe (1203) is in an inverted U-shaped structure.

8. The method for preparing a protein polypeptide graft for enhancing immunity according to claim 1, characterized in that: The protein-peptide graft in the preparation method of the protein-peptide graft for enhancing immunity comprises the following raw materials in parts by weight: The milk protein contains casein, 60-70 parts of albumin, 5-10 parts of egg white protein, and 20-30 parts of polysaccharide; The polysaccharide formula includes 5 parts of isomaltooligosaccharide, 4 parts of fructooligosaccharide and 1 part of stachyose.

9. The method for preparing a protein polypeptide graft for enhancing immunity according to claim 1, characterized in that: In the step 1, the output speed of the speed regulating motor (6), i.e., the speed of the first stirring shaft (4), is 150 r / min; In step 2, the rotation speed of the second stirring shaft (7) is 150 r / min; In step three, the stirring speed of the first stirring shaft (4) is 120 r / min, while the speed of the second stirring shaft (7) is kept constant through the gearbox (8).

10. The method for preparing a protein polypeptide graft for enhancing immunity according to any one of claims 1 to 9, characterized in that: The method for preparing the protein polypeptide graft for enhancing immunity is applied in the technical field of nutrient preparation.

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