Hippophae rhamnoides composition for dispelling effects of alcohol, hippophae rhamnoides plant drink as well as preparation

Abstract

The invention discloses a hippophae rhamnoides composition for dispelling effects of alcohol and a hippophae rhamnoides plant The hippophae rhamnoides fruit composition comprises the following components by weight: 16g of hippophae rhamnoides fruit primary pulp, 0.1 g of globe artichoke juice powder, 0.45 g of ampelopsis grossedentata leaf instant powder, 0.01 g of rhizoma curcumae longae, 0.02 g of radix puerariae powder and 0.03 g of hovenia dulcis thunb powder. The hippophae rhamnoides composition for dispelling the effects of alcohol is prepared from the following six ingredients: hippophae rhamnoides, radix puerariae, hovenia dulcis thunb, globe artichoke, ampelopsis grossedentata leaves and turmeric, so that the hippophae rhamnoides composition has the effects of nourishing the liver to promote digestion, clearing heat, promoting diuresis, promoting the circulation of qi, harmonizing blood, dissolving turbidity, reducing lipid and relieving Meanwhile, the hippophae rhamnoides plant drink can effectively relieve shielding damage of ethanol to gastrointestinal tracts, reduce the levels of serum ALT and AST after drinking, relieve oxidative stress of the liver, also has a certain improvement effect on balance movement and learning and memory ability damage caused by drinking, and has a relatively comprehensive hangover alleviating effect.

Description

Technical Field

[0001] The present invention relates to a composition with hangover effect, and specifically to a seabuckthorn plant drink. Background Art

[0002] Excessive drinking has become one of the main public health problems in our country, leading to various systemic diseases. Therefore, it is very important to relieve the impact of alcohol on the human body in a timely manner.

[0003] Traditional Chinese medicine, as a component of natural origin, plays an important role in exerting the hangover effect. Summary of the Invention

[0004] The purpose of the present invention is to solve the defects existing in the prior art and provide a new hangover product to comprehensively reduce the damage caused by alcohol to the body.

[0005] To achieve the above purpose, the present invention provides a seabuckthorn composition for hangover, which comprises the following components in weight ratio: 16 g of seabuckthorn puree, 0.1 g of artichoke juice powder, 0.45 g of instant powder of Ampelopsis grossedentata leaves, 0.01 g of turmeric, 0.02 g of kudzu root powder, and 0.03 g ofhovenia dulcis powder.

[0006] The seabuckthorn composition for hangover of the present invention is composed of seabuckthorn, kudzu root, hovenia dulcis, artichoke, Ampelopsis grossedentata leaves, and turmeric. In the formula, seabuckthorn is used as the monarch drug, nourishing yin and liver, strengthening the stomach and promoting digestion, activating blood circulation and removing stasis. Kudzu root and hovenia dulcis are used as the ministerial drugs, with kudzu root relieving hangover and reducing fever, promoting the production of body fluid and quenching thirst, lifting yang and stopping diarrhea; hovenia dulcis relieving alcohol toxicity, stopping polydipsia, stopping vomiting, and promoting urination and defecation. Artichoke and Ampelopsis grossedentata leaves are used as the assistant drugs, both of which can clear away damp-heat and remove the pathological factors of alcohol-induced damp-heat toxin. Turmeric is used as the adjuvant drug, regulating qi and soothing the liver, promoting blood circulation and removing stasis, and eliminating masses and accumulations under the hypochondrium. The six herbs in this composition are used together to achieve the effects of nourishing the liver and promoting digestion, clearing away heat and dampness, regulating qi and activating blood circulation, resolving turbidity and reducing lipid, and relieving the harm of alcohol toxicity.

[0007] The present invention also provides a seabuckthorn plant drink for hangover. Each 120 g of the seabuckthorn plant drink comprises the following components: 9 g of edible glucose, 1.2 g of galactooligosaccharide, 16 g of seabuckthorn puree, 0.1 g of artichoke juice powder, 5 g of ultra-concentrated lactic acid bacteria fermentation stock solution, 1 g of concentrated apple juice, 0.45 g of instant powder of Ampelopsis grossedentata leaves, 0.01 g of turmeric, 0.02 g of kudzu root powder, 0.0332 g of rice bran alkanol, 0.03 g of hovenia dulcis powder, 0.16 g of pectin, 0.35 g of konjac powder, 0.005 g of betaine, 0.0001 g of vitamin B6, 0.0012 g of nicotinamide, 0.01 g of corn oligopeptide powder, 0.12 g of food flavor, 0.004 g of stevioside, and the rest is water.

[0008] Among them, the ultra-concentrated lactic acid bacteria fermentation stock solution is made from seven probiotics (Lacticaseibacillus casei, Lactiplantibacillus plantarum, Lactobacillus delbrueckii subsp. bulgaricus, Streptococcus salivarius subsp. thermophilus, Lactobacillus helveticus, Bifidobacterium animalis subsp. lactis, Lactobacillus acidophilus) through long-term, high-concentration, and high-activity fermentation, and is refined by a special formula and process. The ultra-concentrated lactic acid bacteria fermentation stock solution is produced by Luohe Weikang Biotechnology Co., Ltd.

[0009] This invention mainly uses the seabuckthorn composition to reduce the damage of alcohol to the body. At the same time, ultra-concentrated lactic acid bacteria fermentation stock solution and galactooligosaccharides are added, which can produce effects such as improving constipation, regulating intestinal flora, enhancing immunity, and promoting mineral absorption after being fermented and utilized by intestinal microorganisms. And rice bran aliphatic alcohol is used to regulate blood lipids, reduce inflammation, relieve fatigue, and improve immunity.

[0010] This invention also provides a preparation method for the above-mentioned seabuckthorn plant drink for relieving hangover, including the following steps:

[0011] (1) Premix the weighed pectin and konjac powder according to the weight ratio.

[0012] (2) Add the weighed water into the emulsifying tank, reserve part of the water for rinsing the subsequent material buckets and material bags, start stirring, with a stirring speed of 30 r / min, heat with steam, raise the temperature to 60 °C, and stop stirring; slowly and evenly add the premixed pectin and konjac powder, and emulsify and stir for 5 minutes, with a stirring speed of 2500 r / min.

[0013] (3) After the emulsifying and stirring is completed, continue to add edible glucose, instant powder of Ampelopsis grossedentata, artichoke juice powder, turmeric, kudzu root powder, rice bran aliphatic alcohol,hovenia powder, corn oligopeptide powder, and stevioside according to the weight ratio. After rinsing the material bag with part of the reserved water and adding it, start emulsifying and stirring again, with a stirring time of 5 minutes and a stirring speed of 2500 r / min.

[0014] (4) After the stirring is completed, continue to add galactooligosaccharides, seabuckthorn juice, ultra-concentrated lactic acid bacteria fermentation broth, concentrated apple juice, niacinamide, vitamin B6, betaine, food flavoring according to the weight ratio, wash the material barrel with a part of the reserved water and then add it, and add the remaining reserved water. After the feeding is completed, enter the blending stage; (5) Blending: After the feeding is completed, start stirring and steam heating, emulsify and stir for 5 minutes, and the stirring speed is 30 r / min; heat the liquid to 80 °C and keep it warm for 10 minutes.

[0015] The present invention also provides the application of the above-mentioned seabuckthorn plant drink for relieving hangover in the preparation of hangover drugs.

[0016] Furthermore, the seabuckthorn plant drink for relieving hangover of the present invention can be used to prepare drugs for treating gastrointestinal tissue damage, liver damage, motor balance and learning and memory damage caused by drinking.

[0017] The present invention has the following advantages compared with the prior art:

[0018] The present invention uses seabuckthorn, kudzu root, oriental raisin tree seed, artichoke, ampelopsis grossedentata leaf, and turmeric in a formula. Seabuckthorn nourishes yin and soothes the liver, strengthens the spleen and stomach; kudzu root relieves hangover and reduces fever; oriental raisin tree seed clears the chest diaphragm; artichoke soothes the liver and gallbladder, clears and discharges damp-heat; ampelopsis grossedentata clears heat and detoxifies, promotes diuresis and reduces swelling; turmeric regulates qi and soothes the liver, promotes blood circulation and removes stasis, and eliminates masses and accumulations under the hypochondrium. The six herbs are used together to achieve the effects of nourishing the liver and promoting digestion, clearing heat and promoting diuresis, regulating qi and activating blood circulation, removing turbidity and reducing lipid, and relieving the harm of alcohol poisoning.

[0019] As a medicine and food homologous compound, the seabuckthorn plant drink of the present invention can effectively reduce the damage of ethanol to the gastrointestinal barrier, lower the levels of serum ALT and AST after drinking, reduce liver oxidative stress, and also has a certain improvement effect on the impaired balance movement and learning and memory ability caused by drinking. It has a relatively comprehensive hangover effect and provides a new idea for the research and development of new hangover products. Description of the Drawings

[0020] Figure 1 It is the process flow chart for the preparation of the seabuckthorn plant drink of the present invention;

[0021] Figure 2 It is the influence of different test groups in Example 2 of the present invention on the serum transaminase activity of alcohol-fed rats;

[0022] Figure 3 It is the influence of different test groups in Example 2 of the present invention on the serum triglyceride level of alcohol-fed rats;

[0023] Figure 4 It is the influence of different test groups in Example 2 of the present invention on the serum MDA and liver MDA contents of alcohol-fed rats;

[0024] Figure 5Effects of different experimental groups in Example 2 of the present invention on the contents of serum D-lactic acid and endotoxin in alcohol-fed rats;

[0025] Figure 6 Effects of different experimental groups in Example 2 of the present invention on the liver weight of alcohol-fed rats;

[0026] Figure 7 Effects of different experimental groups in Example 2 of the present invention on oxidative stress in alcohol-fed rats;

[0027] Figure 8 Effects of different experimental groups in Example 2 of the present invention on the gastric histopathology of alcohol-fed rats.

[0028] Figures 2 - 8 In, CON, JJY-H, JJY-M: N = 10; MOD: N = 8; HWJZ, JJY-L: N = 9; **P < 0.01, compared with CON; # P < 0.05, ## P < 0.01, compared with MOD. Detailed implementation manners

[0029] The present invention will be described in detail below in conjunction with specific embodiments.

[0030] Example 1

[0031] Product preparation example

[0032] Raw materials: Edible glucose is purchased from Qinhuangdao Lihua Starch Co., Ltd., galactooligosaccharide is purchased from FrieslandCampina Food (Shanghai) Co., Ltd., seabuckthorn puree is purchased from Xinjiang Dar Biology Technology Co., Ltd., artichoke juice powder is purchased from Shanghai Gexin Trading Co., Ltd., ultra-concentrated lactic acid bacteria fermentation stock solution is purchased from Luohe Weikang Biology Technology Co., Ltd., concentrated apple juice is purchased from Zhangpu Dachuan Food Industry Co., Ltd., instant powder of Ampelopsis grossedentata leaves is purchased from Huangshan Huilvyuan Biology Technology Co., Ltd., turmeric is purchased from Chr. Hansen (Beijing) Trading Co., Ltd., kudzu root powder is purchased from Shaanxi Bolin Biology Technology Co., Ltd., rice bran alkanol is purchased from Shanghai Tongyuan Food Technology Co., Ltd., powder of Hovenia dulcis Thunb. is purchased from Hanzhong Tianrang Valley Biology Technology Co., Ltd., pectin is purchased from Yantai Andeli Pectin Co., Ltd., konjac powder is purchased from Zhejiang Shangfang Biology Technology Co., Ltd., betaine is purchased from Danisco (China) Co., Ltd., vitamin B6 is purchased from Jiangxi Tianxin Pharmaceutical Co., Ltd., nicotinamide is purchased from Guangzhou Lonza Pharmaceutical Co., Ltd., corn oligopeptide powder is purchased from Wuhan Tiantianhao Biological Products Co., Ltd., food flavor is purchased from Hasegawa Flavors (Suzhou) Co., Ltd., steviol glycoside is purchased from Shandong Aojing Biology Technology Co., Ltd.

[0033] As Figure 1 shown, the preparation method of the seabuckthorn plant drink is as follows:

[0034] (1) Weigh the components according to the formula in Table 1 (120 g per bag).

[0035] Table 1 Formula of Seabuckthorn Plant Drink

[0036]

[0037]

[0038] (2) Premix pectin and konjac powder.

[0039] (3) Add the weighed water into the emulsifying tank, reserve part of the water for rinsing the subsequent material buckets and bags, turn on the stirring, the stirring speed is 30 r / min, heat with steam, raise the temperature to 60 °C, and turn off the stirring; slowly and evenly put into the emulsifying tank the premixed material of pectin and konjac powder, and carry out emulsifying stirring for 5 minutes, the stirring speed is 2500 r / min;

[0040] (4) After the emulsifying stirring is completed, slowly and evenly put into the emulsifying tank the solid materials (edible glucose, instant powder of Ampelopsis grossedentata leaves, artichoke juice powder, turmeric, kudzu root powder, rice bran alkanol, powder of Hovenia dulcis, corn oligopeptide powder, steviol glycoside) except for essence and fortifier without sequence, and also put into the emulsifying tank the part of the reserved water for rinsing the material bag, turn on the emulsifying stirring again, the stirring time is 5 minutes, and the stirring speed is 2500 r / min. (5) After the stirring is completed, slowly and evenly put into the formula the liquid materials (galactooligosaccharides, seabuckthorn juice, super-concentrated lactic acid bacteria fermentation broth, concentrated apple juice), and put into the emulsifying tank after rinsing the material bucket with part of the reserved water, finally put into the emulsifying tank the nutritional fortifier, spices (niacinamide, vitamin B6, betaine, food essence), and the remaining reserved water, and enter the blending process after the feeding is completed;

[0041] (6) Blending: After the feeding is completed, turn on the stirring and turn on the steam heating, carry out emulsifying stirring for 5 minutes, the stirring speed is 30 r / min; raise the temperature of the liquid material to 80 °C and keep it warm for 10 minutes.

[0042] (7) Inspection and canning: Take samples to detect the pH value and soluble solids, turn on the stirring after the sampling is completed, the stirring speed is 30 r / min; pump the qualified liquid material through a filter screen (20 mesh) into the buffer tank, and then through canning, water bath sterilization, outer packaging, inspection and then storage.

[0043] Example 2

[0044] Effect Example

[0045] 1. Experimental Instruments

[0046] BSA124S Electronic Balance (0.1 mg - 120 g), Sartorius Scientific Instruments (Beijing) Co., Ltd.; LegendMicro17R High-Speed Refrigerated Centrifuge, Thermo Fisher Scientific (China) Co., Ltd.; TDZ4-WS Low-Speed Tabletop Centrifuge, Shanghai Luhangyi Centrifuge Instrument Co., Ltd.; Unique-R20 Multi-Functional Ultra-Pure Water System, Resistech Water Purification Technology Co., Ltd.; ZS-RDM-DS Rat Rotarod Fatigue Tester, ZS-MGY Y-Maze, Beijing Zhongshi Ditech Technology Development Co., Ltd.; KZ-III-F Low-Temperature High-Speed Tissue Grinder, Wuhan Sevier Biotechnology Co., Ltd.; Varioskan TM LUX Multi-Functional Microplate Reader, Thermo Scientific, USA; Donatello Dehydrator, DIAPATH; JB-P5 Embedding Machine, Wuhan Junjie Electronics Co., Ltd.; RM2016 Microtome, Shanghai Leica Instruments Co., Ltd. Tissue; KD-P Spreading Machine, Jinhua Cody Instrument and Equipment Co., Ltd., Zhejiang Province; GFL-230 Oven, Tianjin Laiborui Instrument and Equipment Co., Ltd.; BX53 Microscope, Olympus, Japan.

[0047] 2. Experimental Materials

[0048] Hongxing Erguotou 56-degree Light Aroma Baijiu (500 mL / bottle), Beijing Hongxing Co., Ltd.; 0.9% Sodium Chloride Injection, Anhui Shuanghe Pharmaceutical Co., Ltd., Batch No.: 2212081; Ethanol (ALC), Triglyceride (TG), Malondialdehyde (MDA), Reduced Glutathione (GSH), Total Superoxide Dismutase (SOD), Alcohol Dehydrogenase (ADH), D-Lactic Acid (D-LAC), Endotoxin Detection Kit, Nanjing Jiancheng Bioengineering Institute; Alanine Aminotransferase (ALT), Aspartate Aminotransferase (AST) Detection Kit, Neusoft Witman Biotechnology (Nanjing) Co., Ltd.; Rat Complete Basic Granular Feed, Jiangsu Xietong Pharmaceutical Biotechnology Co., Ltd.; Corncob Particle Bedding, Yizheng Anlimao Biotechnology Co., Ltd.

[0049] 3. Experimental Animals

[0050] SD Rats, SPF grade, weighing 190 - 230 g, male, purchased from Jiangsu Qinglongshan Biotechnology Co., Ltd., Production License No.: SCXK (Su) 2024 - 0001. The rats were raised in the Animal Center of China Pharmaceutical University (Animal Use License No.: SYXK (Su) 2021 - 0011). The breeding laboratory temperature was 24 ± 2°C; relative humidity was 40% - 70%; the number of air exchanges per hour was 10 - 15 times / hour; the light cycle was 12 (day) / 12 (night) hours, and no more than 3 rats were kept in each cage.

[0051] 4. Experimental Methods

[0052] For the data processing and statistical analysis in the following test examples, the data are expressed as mean ± standard deviation (M ± SD). The comparison between groups was performed using the student-t test, and P < 0.05 was considered as a statistically significant difference.

[0053] 4.1 Sample Preparation and Dose Setting

[0054] Seabuckthorn Plant Drink (JJY) (Batch number: 2024 / 10 / 11), 120 g (120 ml) / bag, one bag per day. Converted to rats according to body surface area, it is equivalent to 12 g (12 mL)·kg -1 . Before use, it was evaporated and concentrated to prepare a 6:1 concentrated solution and given to rats. Set 2 mL·kg -1 , 4 mL·kg -1 and 8 mL·kg -1 and other 3 dose groups, which are approximately equivalent to 1, 2, and 4 times the human dosage respectively. Before administration, the concentrated solution was appropriately diluted with distilled water so that the administration volume of the 3 doses was all 10 mL·kg -1 .

[0055] Positive control: "Haiwang Jinzun" Oyster Soy Peptide Carnitine Oral Liquid (HWJZ) (Batch number: 20231136), 50 ml / bottle, two bottles per day. Converted to rats according to body surface area, it is equivalent to 10.42 mL·kg -1 . The dose in this experiment was set at 10 mL·kg -1 , which is approximately equivalent to 1 times the human dosage.

[0056] 4.2 Establishment of Alcohol Intoxication Model

[0057] After the rats were purchased and adapted for 1 week of feeding, they were intragastrically administered 56° white wine at a dose of 10 - 20 ml / kg. The "righting reflex" of the rats was used to judge the state of intoxication and sobering up of the rats. That is, after the rats were intragastrically administered alcohol, they were placed on the animal cage with their backs down. If the back-down posture lasted for more than 30 s, it was considered that the righting reflex disappeared, which means intoxication. Record the number of intoxicated rats and the number of dead rats, and select the intragastric alcohol dose with the highest number of intoxicated rats and the lowest number of dead rats to establish a rat alcohol intoxication model. After preliminary experiments, 18 mL·kg -1 of alcohol intragastric administration was selected as the modeling for the formal experiment.

[0058] 4.3 Grouping and Administration

[0059] Randomly select 60 rats and divide them into 6 groups according to body weight, with 10 rats in each group: (1) Normal control group (CON), (2) Alcohol intoxication model group (MOD), (3) Haiwang Jinzun positive control group (HWJZ, 20 ml / kg), (4) High-dose group of Seabuckthorn Plant Drink (JJY-H, 8 ml concentrated solution·kg-1 ), (5) Medium-dose group of seabuckthorn plant drink (JJY-M, 4 ml of concentrated liquid·kg -1 ), (6) Low-dose group of seabuckthorn plant drink (JJY-L, 2 ml of concentrated liquid·kg -1 ).

[0060] Rats in each group were intragastrically administered the corresponding test substances once a day, and the administration volume was 20 mL·kg -1 . Rats in the normal group and the model group were given the same volume of distilled water. At 8 pm on the 4th day of administration, the rats were fasted overnight without water deprivation. At 8 am on the 5th day, 1 hour after intragastric administration of the test substances, except for the normal control group, the other 5 groups were intragastrically administered 56° white liquor at 18 ml / kg once to establish a drunken model.

[0061] 4.4 Determination of drunkenness and drunken time in rats

[0062] After intragastric administration of alcohol to rats in each group, the activity was observed and timed, and the disappearance time and recovery time of the righting reflex of the drunken rats were recorded in a timely manner. The time from alcohol intragastric administration to the disappearance of the righting reflex was recorded as the drunken latency period, and the time from the disappearance of the righting reflex to the recovery of the righting reflex was recorded as the drunken time.

[0063] After intragastric administration of 56° white liquor at 18 mL·kg -1 to the rats in the model group, all 10 rats showed drunken behavior, and 2 rats died after 10 hours of drunkenness. After intragastric administration of white liquor to the high-, medium-, and low-dose groups of seabuckthorn plant drink (hereinafter referred to as JJY-H group, JJY-M group, and JJY-L group), 5, 3, and 2 rats, respectively, did not show drunken behavior, and 1 rat in the low-dose group died after 10 hours of drunkenness. One rat in the HWJZ group did not show drunken behavior, and 1 rat died after 10 hours of drunkenness.

[0064] Compared with the model group (MOD group), the JJY-H group, JJY-M group, and JJY-L group could significantly prolong the drunken latency period of the drinking rats and significantly shorten the drunken time (P<0.01, P<0.05); the JJY-L group could also significantly increase the drunken latency period (P<0.05) and partially shorten the drunken time. The HWJZ group showed a tendency to improve the drunken latency period and drunken time of the rats, but neither reached a statistically significant difference (Table 2).

[0065] Table 2. Effects of seabuckthorn plant drink on drunkenness in drinking rats (M±SD)

[0066]

[0067] / : Not detected; a : Only calculate the data of drunken animals; *P<0.05, **P<0.01, compared with CON;# P < 0.05, ## P < 0.01, compared with MOD.

[0068] 4.5 Determination of ethanol content in rat serum and transaminase activity

[0069] After 12 h of intragastric administration of alcohol to rats in each group, blood was collected from the orbital sinus, allowed to stand at room temperature for 40 min, and centrifuged at 3000 rpm for 10 min to separate the serum. The alcohol concentration in the blood of rats in each group was detected using a blood ethanol kit to investigate the effect of the test substance on the blood ethanol elimination rate. Another part of the serum was taken, and the activities of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were detected using a kit to investigate the protective effect of the test substance on alcohol-induced liver cell damage.

[0070] Compared with the rats in the CON group, after 12 h of intragastric administration of alcohol, the serum ethanol content in the rats in the MOD group was significantly increased (P < 0.01). Compared with the rats in the MOD group, the JJY-H group, JJY-M group, and HWJZ group could all significantly reduce the serum ethanol content in the alcohol-drinking rats (P < 0.01, P < 0.05), and the serum ethanol content in the rats in the JJY-L group also decreased partially (as shown in Table 3).

[0071] Table 3. Effect of seabuckthorn plant drink on serum ethanol content in alcohol-drinking rats (M±SD)

[0072]

[0073]

[0074] **P < 0.01, compared with CON; # P < 0.05, ## P < 0.01, compared with MOD.

[0075] Compared with the rats in the CON group, after 12 h of intragastric administration of alcohol, the activities of serum ALT and AST in the rats in the MOD group were significantly increased (P < 0.01), indicating obvious liver cell damage caused by alcohol. Compared with the MOD group, the JJY-H group, JJY-M group, JJY-L group, and HWJZ group could all significantly reduce the activities of serum ALT and AST (P < 0.01, P < 0.05) (as Figure 2 shown).

[0076] 4.6 Rat behavioral tests

[0077] After 24 h of intragastric administration of alcohol to rats in each group, a rotarod fatigue tester and a Y-maze test were performed. The falling time of the rotarod and the spontaneous alternation rate were used as evaluation indexes respectively to investigate the effect of the test substance on the motor balance and learning and memory ability of drunken rats.

[0078] The detection results of the rotating rod fatigue tester showed that, compared with the rats in the CON group, the dropping time of the rats significantly shortened 24 h after intragastric administration of alcohol, suggesting that alcohol consumption caused certain damage to the motor balance ability of the rats. Compared with the MOD group, both the JJY-H group and the JJY-M group could significantly prolong the dropping time of the alcohol-consuming rats on the rotating rod (P<0.01); the JJY-L group and the HWJZ group had no significant effect on the dropping time of the model rats on the rotating rod.

[0079] The detection results of the Y maze showed that, compared with the rats in the CON group, the spontaneous alternation rate of the rats significantly decreased 24 h after intragastric administration of alcohol, suggesting that alcohol consumption caused certain damage to the spatial learning and memory ability of the rats. Compared with the MOD group, both the JJY-H group and the JJY-M group could significantly prolong the dropping time of the alcohol-consuming rats on the rotating rod (P<0.01, P<0.05); the JJY-L group and the HWJZ group also had a partial improvement effect on the spontaneous alternation rate of the model rats, but neither reached statistical significance (as shown in Table 4).

[0080] Table 4. Effects of seabuckthorn plant drink on the behavior of alcohol-consuming rats (M±SD)

[0081]

[0082]

[0083] **P<0.01, compared with CON; # P<0.05, ## P<0.01, compared with MOD.

[0084] 4.7. Detection of serum biochemical indexes

[0085] After intragastric administration of alcohol for 24 h, the body weight was measured. The rats were anesthetized with urethane 1.0 g·kg -1 (ip), blood was collected from the abdominal aorta, left to stand at room temperature for 40 min, centrifuged at 3000 rpm for 10 min to separate the serum, and the levels of malondialdehyde (MDA), triglyceride (TG), endotoxin and D-lactic acid were detected using the corresponding kits respectively to investigate the improvement effects of the test substances on oxidative stress, adipose tissue hydrolysis and intestinal barrier in the drunken rats.

[0086] Compared with the rats in the CON group, after intragastric administration of alcohol for 24 h, the serum TG level of the rats significantly increased (P<0.01), indicating that excessive alcohol intake caused obvious lipolysis and increased the serum TG level. Compared with the rats in the MOD group, both the JJY-H group and the JJY-M group could significantly reduce the serum TG level (P<0.05); the JJY-L group and the HWJZ group had no significant effect on the serum TG level of the model rats (as Figure 3 shown).

[0087] Compared with the rats in the CON group, after intragastric administration of alcohol for 24 h, the serum MDA content in the rats increased significantly (P<0.05), suggesting that excessive alcohol intake caused oxidative stress in the body. Compared with the MOD group, the JJY-H group could significantly reduce the serum MDA content in the alcohol-drinking rats (P<0.05). There was no obvious change in the serum MDA content in the rats of the JJY-M group, JJY-L group and HWJZ group (as Figure 4 shown).

[0088] Compared with the rats in the CON group, after intragastric administration of alcohol for 24 h, the serum D-lactic acid and endotoxin contents in the rats both increased significantly (P<0.01), suggesting that excessive alcohol intake caused damage to the intestinal barrier. Compared with the MOD group, JJY could significantly reduce the serum D-lactic acid and endotoxin contents in the alcohol-drinking rats (P<0.01, P<0.05), and the improvement effect had a certain dose-dependence. HWJZ had no obvious improvement effect on the serum D-lactic acid and endotoxin contents in the model rats (as Figure 5 shown).

[0089] 4.8. Detection of liver alcohol dehydrogenase activity and oxidative stress

[0090] After blood collection, the rats were sacrificed by cervical dislocation. The liver was dissected and weighed, and the liver weight index [liver weight (g) / body weight (g)×100%] was calculated. Take a part of fresh liver tissue and make a 10% tissue homogenate by mechanical homogenization under ice-water bath conditions according to the ratio of liver weight (g): 0.9% normal saline (mL)=1:9. Centrifuge at 3000 rpm / min for 10 min, and take the supernatant part after centrifugation. Use the corresponding kits to test the activity of liver alcohol dehydrogenase (ADH), as well as the activities of total superoxide dismutase (SOD), MDA content and reduced glutathione (GSH) content. Investigate the effects of the test substances on the ethanol catabolism and oxidative stress of the rats' livers.

[0091] Compared with the rats in the CON group, after intragastric administration of alcohol, the body weight of the rats decreased, and at the same time, the liver weight and weight index also decreased significantly (P<0.01, P<0.05). The JJY-H group, JJY-M group, JJY-L group and HWJZ group had no significant effects on the body weight and liver weight of the MOD group (as Figure 6 shown).

[0092] Compared with the rats in the CON group, after intragastric administration of alcohol for 24 h, the activity of liver alcohol dehydrogenase in the rats increased significantly (P<0.01). The JJY-H group, JJY-M group, JJY-L group and HWJZ group had no significant effects on the activity of liver alcohol dehydrogenase in the rats of the MOD group (as shown in Table 5).

[0093] Table 5. Effects of sea buckthorn plant drink on alcohol metabolism in alcohol-drinking rats (M±SD)

[0094]

[0095] **P < 0.01, compared with CON; # P < 0.05, ## P < 0.01, compared with MOD.

[0096] Compared with the rats in the CON group, after intragastric administration of alcohol for 24 h, the content of MDA in the liver of rats increased significantly (P < 0.01) (as Figure 4 shown), while the activities of SOD and the content of GSH decreased significantly (P < 0.01), suggesting that excessive alcohol intake caused obvious oxidative stress response in the liver. Compared with the rats in the MOD group, JJY could significantly reduce the content of MDA in the liver of alcohol-drinking rats, increase the activities of SOD and the content of GSH, and the improvement had a certain dose-dependence. HWJZ also had an obvious improvement effect on the indexes of oxidative stress in the liver of model rats (P < 0.05) (as Figure 7 shown).

[0097] 4.9. Histopathological examination of the stomach

[0098] Dissect and take out the stomach tissue, fix it with 10% formalin, make routine sections, stain with HE, and observe the morphology and structure of the gastric mucosa tissue under a light microscope to evaluate the protective effect of the test substance on alcohol-induced gastric mucosal damage.

[0099] The results of pathological examination showed that the structures of each layer of the gastric tissue of the rats in the CON group were clear, the mucosal epithelium was intact, there was no obvious necrosis, exfoliation or ulcer formation, there was no obvious congestion or edema in the submucosa, no obvious inflammatory cell infiltration was seen, the muscular layer structure was intact, and there was no inflammatory exudate on the serosa. The gastric tissue of the rats in the MOD group showed focal necrosis and exfoliation of the mucosal epithelium to varying degrees, a varying number of inflammatory cell infiltrations were visible in the lamina propria, the submucosa was slightly edematous, and a small amount of inflammatory cell infiltrations were seen in the submucosa in some areas. The muscular layer structure was intact, and there was no inflammatory exudate on the serosa. Compared with the model group, the JJY group had a certain improvement effect on the histological damage of the gastric mucosa of rats (as Figure 8 shown).

[0100] Alcohol (ethanol) is a substance with a wide range of biological activities, and its in vivo metabolic process and physiological effects involve multiple organ systems. A large number of studies have shown that acute alcohol poisoning caused by excessive drinking has clear damage to the stomach, intestines, liver, brain, etc. Moreover, these damaged organs not only have independent damage processes but also have certain internal connections and mutual influences (especially the intestinal barrier and liver damage. The former can lead to the entry of toxins and harmful substances in the intestine into the blood, affecting the whole body; the latter damage weakens the liver's detoxification function, increasing the risk of harmful substances in the intestine). In this invention, a rat drunkenness model is established to comprehensively evaluate the hangover effect of the seabuckthorn plant drink. In this example, after the rats drank alcohol, focal necrosis and exfoliation of the mucosal epithelium occurred to varying degrees in the gastric mucosa tissue, and the submucosa was slightly edematous. Compared with the model group, the histological damage of the gastric mucosa tissue of the rats in the JJY group had a certain improvement effect, and was accompanied by an increase in the serum D-lactic acid and endotoxin contents, indicating that obvious gastrointestinal tissue damage and impaired barrier function occurred in the model rats, and the seabuckthorn plant drink of this invention had an obvious improvement effect on this, suggesting that this product can reduce the damage of ethanol to the gastrointestinal barrier. In addition, the serum ethanol concentration in the seabuckthorn plant drink intervention group was significantly lower than that in the model group, but there was no obvious effect on the activity of liver alcohol dehydrogenase. This result suggests that this product may mainly slow down the gastrointestinal absorption of ethanol through gastrointestinal protection, thereby reducing the in vivo ethanol exposure after drinking.

[0101] After drinking, ethanol is rapidly absorbed through the gastrointestinal tract (20% by the stomach and 80% by the small intestine), and the blood peak concentration is reached in 30 - 90 minutes. The absorption rate of ethanol is affected by various factors, such as the integrity of the gastrointestinal barrier, drinking speed, fasting or fullness state, alcohol concentration, etc. As the main site of ethanol absorption, it is also directly stimulated by ethanol, making the mucus layer of the mucosa thinner, weakening its protective barrier function, and thus increasing the gastrointestinal absorption of ethanol. The seabuckthorn plant drink of this invention can reduce the serum ALT and AST levels of model rats and alleviate liver oxidative stress, indicating that this product has a good improvement effect on liver damage caused by drinking.

[0102] The hydrophilic property of ethanol makes its distribution volume (0.6 L / kg) close to the total body water, and it easily penetrates the blood-brain barrier, thus producing drunkenness effects related to the nervous system. The specific manifestations include: weakened cognitive control ability, decreased attention and perception ability, limited motor balance ability, etc. The seabuckthorn plant drink of this invention can significantly reduce the occurrence of drunkenness in rats after intragastric administration of white liquor, and at the same time extend the drunkenness latency period of the drinking rats and shorten the drunkenness time, showing a good preventive effect against drunkenness. In addition, the seabuckthorn plant drink also has an obvious improvement effect on the impaired balance movement and learning and memory ability of rats caused by drinking, suggesting that this product can reduce the hangover effect of drinking.

[0103] In summary, the results of this experiment showed that administering seabuckthorn plant drink before alcohol intake could significantly reduce the serum ethanol content in model rats, decrease the incidence of drunkenness in rats and shorten the drunkenness time, and had varying degrees of improvement effects on hepatocyte toxicity, gastrointestinal barrier damage, oxidative stress, lipid metabolism disorder, motor balance and learning and memory impairment in rats caused by excessive alcohol intake. Thus, it can be seen that seabuckthorn plant drink can reduce the amount of alcohol exposure in the body during excessive drinking, and at the same time alleviate the adverse effects on tissues and organs such as the liver, gastrointestinal tract and brain caused by excessive alcohol intake, and has a relatively comprehensive hangover effect.

Claims

1. A seabuckthorn composition for sobering up, characterized in that: The seabuckthorn composition comprises the following components in weight ratio: 16g of seabuckthorn puree, 0.1g of artichoke juice powder, 0.45g of Ampelopsis grossedentata leaf instant powder, 0.01g of turmeric, 0.02g of kudzu root powder, and 0.03g of Hovenia dulcis powder.

2. A seabuckthorn plant drink for hangover relief, characterized in that: Every 120 grams of sea buckthorn plant drink includes the following components: 9g edible glucose, 1.2g galacto-oligosaccharide, 16g sea buckthorn puree, 0.1g artichoke juice powder, 5g super concentrated lactic acid bacteria fermentation stock solution, 1g concentrated apple juice, 0.45g Agave vulgaris leaf instant powder, 0.01g turmeric, 0.02g kudzu root powder, 0.0332g rice bran fatty alcohol, 0.03g Hovenia dulcis powder, 0.16g pectin, 0.35g konjac powder, 0.005g betaine, 0.0001g vitamin B6, 0.0012g niacinamide, 0.01g corn oligopeptide powder, 0.12g food flavoring, 0.004g stevioside, and the rest is water.

3. The method for preparing the seabuckthorn plant drink for hangover relief according to claim 2, characterized in that: The following steps are involved: Premix the weighed pectin and konjac flour according to the weight ratio; Add weighed water to the emulsification tank, reserve some water for subsequent rinsing of material barrels and material bags, start stirring at a speed of 30r / min, steam heating, raise the temperature to 60°C, and turn off stirring; slowly and evenly add the premix of pectin and konjac flour, emulsify and stir for 5 minutes at a speed of 2500r / min; After the emulsification and stirring is completed, continue to add edible glucose, Agave sphenanthera leaf instant powder, artichoke juice powder, turmeric, kudzu root powder, rice bran fatty alcohol, Hovenia dulcis powder, corn oligopeptide powder, and steviol glycoside according to the weight ratio, and add some reserved water to rinse the material bag before adding, and start the emulsification and stirring again. The stirring time is 5 minutes and the stirring speed is 2500r / min.

4. After the stirring is completed, continue to add galacto-oligosaccharide, sea buckthorn juice, ultra-concentrated lactic acid bacteria fermentation stock solution, concentrated apple juice, niacinamide, vitamin B6, betaine, food flavoring, and part of the reserved water to rinse the material barrel according to the weight ratio, and then add the remaining reserved water. After the feeding is completed, enter the deployment stage; Preparation: After the feeding is completed, start stirring and steam heating, emulsify and stir for 5 minutes at a stirring speed of 30r / min; raise the temperature of the liquid to 80℃ and keep it warm for 10 minutes.

5. Use of the seabuckthorn plant drink for sobering up as claimed in claim 2 in preparing a drug for sobering up.

6. Use of the seabuckthorn plant drink for alcohol sobering up as claimed in claim 2 in the preparation of medicines for treating gastrointestinal tissue damage, liver damage, motor balance and learning and memory damage caused by drinking.

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