Preparation method of semi-bionic extraction plant skin care raw material

Abstract

The invention discloses a preparation method of a semi-bionic extraction plant skin care raw material, and relates to the technical field of plant raw materials. The preparation method of the semi-bionic extraction plant skin care raw material comprises the following specific steps: S1, preparation of a percolation extracting solution: mixing hydrolyzed keratin (1%-5%), hydrolyzed collagen (1%-5%) and plant ceramide (1%-5%), and putting the mixture into water; s2, plant material pretreatment: drying the plant material for extraction at 60 DEG C, then crushing, and sieving with a 40-mesh sieve to obtain uniform plant powder; s3, percolation extraction: loading the pretreated plant powder into a percolation column, and performing percolation extraction by using a prepared percolation extracting solution according to a liquid-material ratio of (10-20): 1; s4, secondary ethanol reflux extraction: taking the dried percolation extract, adding a 50-80% ethanol aqueous solution with the amount of 10-20 times that of the dried percolation extract, and carrying out heating reflux extraction twice; and S5, resin purification and enrichment: purifying the concentrated paste by adopting D101 macroporous adsorption resin.

Description

Technical Field

[0001] The present invention relates to the technical field of plant raw materials, and particularly relates to a preparation method of a semi-bionic extraction plant skin care raw material. Background Art

[0002] Natural components contained in plants, such as polyphenols, flavonoids, saponins, alkaloids, terpenoids, etc., have significant antibacterial, anti-inflammatory, analgesic, antipruritic, antioxidant, anti-aging and other effects, and are widely used in the skin care field. They are increasingly favored by functional cosmetics due to their unique biological activity and safety. However, the chemical structures of these active ingredients vary widely, and there are obvious differences in their adsorption ability and transdermal absorption rate on the skin. Only those active substances that can stay adsorbed on the skin for a longer time and have a better transdermal absorption effect can truly exert their biological functions on the skin.

[0003] The current extraction methods of plant raw materials mainly include water distillation method, solvent extraction method, ultrasonic extraction method, microwave extraction method, etc. The selection of these methods depends on the nature of the raw materials and the characteristics of the required extracts. By the principle of solvent similar dissolution, a certain type of chemical substance in the plant is enriched, but the skin-friendly substances in its components cannot be screened. This results in a weak affinity between the raw material and the skin, and poor effects of retention and transdermal absorption on the skin. In order to enhance the efficacy and stability of the extract, some methods add chemical penetration enhancers, such as azone, propylene glycol, salicylic acid, urea, tetrahydropiperine, menthol, borneol, etc. to the extract after obtaining it; or degrade macromolecular substances into small molecular substances through fermentation technology to make them more easily absorbed by the skin. The former added chemical penetration enhancers mainly interfere with the lipid bilayer structure of the stratum corneum, increase lipid fluidity, thereby changing the compound partition coefficient and increasing the penetration amount. However, the introduction of the penetration enhancer often also changes the properties and formulation stability of the product; the latter makes the extraction process more cumbersome, increases the extraction cost, and fermentation changes the structure of the active substances originally contained in the plant, which may lead to changes in its efficacy or even loss of effect.

[0004] Therefore, in the extraction process, it is urgently necessary to select a certain method to directly screen the active substances with strong affinity for the skin, and improve the efficiency of enriching the efficacy skin care raw materials without changing the original active ingredients of the plant, and reduce the extraction cost. Summary of the Invention

[0005] An object of the present invention is to at least solve one of the technical problems existing in the prior art, and provide a preparation method of a semi-bionic extraction plant skin care raw material, which can solve the problems of more cumbersome extraction process and higher extraction cost.

[0006] To achieve the above object, the present invention provides the following technical solution: A preparation method of a semi-bionic extraction plant skin care raw material, comprising the following specific steps. S1 Preparation of percolation extract: Mix hydrolyzed keratin (1%-5%), hydrolyzed collagen (1%-5%), and phytoceramide (1%-5%) and put them into water;

[0007] S2 Pretreatment of plant materials: Dry the plant materials used for extraction at 60°C, then crush them, and pass through a 40-mesh sieve after crushing to obtain uniform plant powder;

[0008] S3 Percolation extraction: Load the pretreated plant powder into a percolation column, and perform percolation extraction with the prepared percolation extract according to the liquid-solid ratio of 10-20:1;

[0009] S4 Secondary ethanol reflux extraction: Take the above-mentioned dried percolation extract, add 10-20 times the amount of 50%-80% ethanol aqueous solution, and perform heating reflux extraction for 2 times;

[0010] S5 Resin purification and enrichment: Purify the concentrated paste with D101 macroporous adsorption resin.

[0011] Preferably, for the preparation of the percolation extract in S1, during specific operation, weigh 1-5 Kg of hydrolyzed keratin, 1-5 Kg of hydrolyzed collagen, and 1-5 Kg of phytoceramide and put them into 100 Kg of water;

[0012] Adjust the pH to 5.5-8.5 with acetic acid-sodium acetate buffer solution, and stir at a constant temperature of 55°C until a clear and transparent colloidal solution is formed;

[0013] Among them, this colloidal solution is used as the percolation extract, and the hydrolyzates of important components of the skin are used to simulate the human skin environment, providing key conditions for the subsequent selective extraction of active substances with strong skin affinity.

[0014] Preferably, for the percolation extraction in S3, control the pH during the extraction process to be 5.5-8.5, the extraction temperature to be 35-45°C, and the percolation flow rate to be 0.1-1 BV / h (0.1-1 column volume of percolate flows out per hour);

[0015] Among them, under such bionic conditions, through hydrogen bond adsorption, intermolecular forces, and ion adsorption, active components with stronger skin affinity are selectively extracted into the percolation extract. Collect the extract and perform concentration and drying treatments to obtain a preliminary percolation extract.

[0016] Preferably, for the secondary ethanol reflux extraction in S4, in this way, further extract the effective components in the percolation extract, improve the purity and active substance content of the extract, then combine the two extraction liquids and perform a concentration operation to obtain a concentrated paste.

[0017] Preferably, for the purification and enrichment of the S5 resin, first, pure water is used for elution to remove impurities in the extract; then, an ethanol aqueous solution of 30%-100% is used for elution, and the active ingredients will be eluted during this process;

[0018] Among them, the part eluted with the ethanol aqueous solution is collected, concentrated and dried again, and finally an extract of semi-bionic extraction plant skin care raw materials with a high transdermal absorption rate is obtained.

[0019] Compared with the prior art, the beneficial effects of the present invention are:

[0020] 1. For the preparation method of the semi-bionic extraction plant skin care raw materials, compared with the semi-bionic extraction technology, the bionic method of the present invention has obvious innovation. The reported bionic extraction methods mainly simulate the human gastrointestinal fluid by adding digestive enzymes, adjusting the pH, etc. for bionic extraction in order to improve the digestive absorption rate of the extract. The bionic method in this patent is to dissolve the hydrolyzates and analogs of important skin proteins and lipids in water, adjust the pH and temperature, and simulate the human skin absorption environment for extraction to improve the skin's absorption rate of the extract. The transdermal absorption rate is increased by 40% - 450% compared with the general reflux extraction method.

[0021] 2. For the preparation method of the semi-bionic extraction plant skin care raw materials, compared with the general extraction method, the main difference of the present invention lies in the different extraction solutions selected. The general extraction method selects water or organic solvents such as ethanol, methanol, ethyl acetate, petroleum ether, etc. as the extraction solution; the solvent used in this patent is a colloidal solution prepared by mixing hydrolyzed keratin, hydrolyzed collagen, and plant ceramide. The characteristic of this solution is that it has a certain selectivity for the extraction target substance and a low solubility for impurities.

[0022] 3. For the preparation method of the semi-bionic extraction plant skin care raw materials, compared with the post-treatment of general skin care raw material extraction, the present invention has certain innovation. Currently, after obtaining the plant skin care raw material extract, it is generally simply treated by direct drying, solvent extraction, centrifugation, etc. In this patent, after obtaining the extract, secondary extraction is carried out, and purification and enrichment are carried out through macroporous resin, which increases the content of the active ingredients in the extract and further improves the stability and safety of the raw materials. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] The present invention will be further described below in conjunction with the drawings and embodiments:

[0024] Figure 1 is the preparation flow chart of the present invention;

[0025] Figure 2 is a schematic diagram of the total alkaloid content and transdermal absorption rate in the extract of Phellodendron amurense bark by different extraction methods of the present invention;

[0026] Figure 3 Schematic diagram of the total triterpenoid saponin content and transdermal absorption rate in the extracts of Aesculus hippocastanum fruits by different extraction methods of the present invention;

[0027] Figure 4 Schematic diagram of the total iridoid glycoside content and transdermal absorption rate in the extracts of Gentiana scabra by different extraction methods of the present invention;

[0028] Figure 5 Schematic diagram of the total flavonoid content and transdermal absorption rate in the extracts of Campsis grandiflora by different extraction methods of the present invention;

[0029] Figure 6 Schematic diagram of the total saponin content and transdermal absorption rate in the extracts of Anemarrhena rhizomes by different extraction methods of the present invention;

[0030] Figure 7 Schematic diagram of the chlorogenic acid content and transdermal absorption rate in the extracts of Eucommia ulmoides leaves by different extraction methods of the present invention. Detailed implementation manners

[0031] This part will describe in detail the specific embodiments of the present invention. The preferred embodiments of the present invention are shown in the drawings. The function of the drawings is to supplement the description of the text part of the specification, enabling people to visually and vividly understand each technical feature and the overall technical solution of the present invention, but it cannot be understood as a limitation on the protection scope of the present invention.

[0032] In the description of the present invention, it should be understood that for the orientation description, such as up, down, front, back, left, right, etc., the orientation or positional relationship indicated is based on the orientation or positional relationship shown in the drawings. It is only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation on the present invention.

[0033] In the description of the present invention, greater than, less than, exceeding, etc. are understood as not including the number itself, and above, below, within, etc. are understood as including the number itself. If there is a description of first and second, it is only for the purpose of distinguishing technical features and cannot be understood as indicating or implying relative importance or implicitly indicating the quantity of the indicated technical features or implicitly indicating the sequence relationship of the indicated technical features.

[0034] In the description of the present invention, unless otherwise clearly defined, words such as setting, installing, connecting, etc. should be understood in a broad sense. Those skilled in the art can reasonably determine the specific meanings of the above words in the present invention in combination with the specific content of the technical solution.

[0035] Please refer to Figures 1-7, the present invention provides a technical solution: a preparation method of a semi-bionic extraction plant skin care raw material, including the following specific steps. S1 Preparation of percolation extract: Mix hydrolyzed keratin (1%-5%), hydrolyzed collagen (1%-5%), and phytoceramide (1%-5%) and put them into water;

[0036] Specifically, during the operation, for example, weigh 1-5 Kg of hydrolyzed keratin, 1-5 Kg of hydrolyzed collagen, and 1-5 Kg of phytoceramide and put them into 100 Kg of water;

[0037] Adjust the pH to 5.5-8.5 using acetic acid-sodium acetate buffer solution, and stir at a constant temperature of 55°C until a clear and transparent colloidal solution is formed;

[0038] Among them, this colloidal solution is used as the percolation extract, and the hydrolyzates of the important components of the skin are used to simulate the human skin environment, providing key conditions for the subsequent selective extraction of active substances with strong skin affinity.

[0039] S2 Pretreatment of plant materials: Dry the plant materials used for extraction at 60°C, then crush them, and pass through a 40-mesh sieve after crushing to obtain uniform plant powder;

[0040] Among them, this step can increase the specific surface area of the plant materials and improve the subsequent extraction efficiency.

[0041] S3 Percolation extraction: Load the pretreated plant powder into a percolation column, and perform percolation extraction with the prepared percolation extract according to the liquid-to-solid ratio of 10-20:1;

[0042] Among them, control the pH during the extraction process to be 5.5-8.5, the extraction temperature to be 35-45°C, and the percolation flow rate to be 0.1-1 BV / h (0.1-1 column volume of percolation liquid flows out per hour);

[0043] Among them, under such bionic conditions, using hydrogen bond adsorption, intermolecular forces, ion adsorption, etc., the active components with stronger skin affinity are selectively extracted into the percolation extract. Collect the extract and perform concentration and drying treatments to obtain a preliminary percolation extract.

[0044] S4 Secondary ethanol reflux extraction: Take the above-mentioned dried percolation extract, add 10-20 times the amount of 50%-80% ethanol aqueous solution, and perform heating reflux extraction 2 times;

[0045] Among them, in this way, the effective components in the percolation extract are further extracted to improve the purity and active substance content of the extract. Then, combine the two extraction liquids and perform concentration operation to obtain a concentrated paste.

[0046] Purification and enrichment of S5 resin: D101 macroporous adsorption resin is used to purify the concentrated paste;

[0047] Among them, first use pure water for elution to remove impurities in the extract; then use 30%-100% ethanol aqueous solution for elution, and the active ingredients will be eluted in this process;

[0048] Among them, collect the part eluted by ethanol aqueous solution, concentrate and dry it again, and finally obtain a semi-bionic extraction plant skin care raw material extract with high transdermal absorption rate.

[0049] Example 1: Extract of Phellodendron amurense Rupr. bark;

[0050] 10 Kg of Phellodendron amurense Rupr. bark is dried at 60 °C, pulverized, and passed through a 40-mesh sieve;

[0051] Weigh 3 Kg of hydrolyzed keratin, 6 Kg of hydrolyzed collagen, and 3 Kg of phytoceramide respectively, put them into 200 Kg of water, adjust the pH to 5.5 with acetic acid-sodium acetate buffer solution, and stir at a constant temperature of 55 °C to form a clear and transparent colloidal solution;

[0052] Put 10 Kg of Phellodendron amurense Rupr. bark powder into the percolation column, carry out percolation extraction with the above 212 Kg of colloidal solution, control the extraction environment temperature at 45 °C, control the percolation flow rate at 0.2 BV / h, collect the percolation extract, concentrate and dry it;

[0053] The above dried percolation extract is heated under reflux with 10 times the amount of 80% ethanol for 2 times, the extraction solutions are combined, concentrated, and the concentrated paste is purified with D101 macroporous adsorption resin. First, elute with pure water for 5 BV, and then elute with 70% ethanol aqueous solution for 14 BV. Collect the part eluted by ethanol aqueous solution, concentrate and dry it to obtain 1.16 Kg of Phellodendron amurense Rupr. bark extract;

[0054] Use the reflux extraction method commonly used in production to obtain the Phellodendron amurense Rupr. bark extract as the reference group for the transdermal absorption rate test. The extraction method is: Phellodendron amurense Rupr. bark powder is heated under reflux with 10 times the amount of 80% ethanol for 2 times, the extraction solutions are combined, concentrated, and dried;

[0055] Using berberine hydrochloride as the standard product, use ultraviolet-visible spectrophotometry to determine the total alkaloid content in the above Phellodendron amurense Rupr. bark extract respectively;

[0056] Determine the transdermal absorption rate of the Phellodendron amurense Rupr. bark extract obtained by the semi-bionic extraction method and the reflux extraction method, and repeat each method 3 times. The experimental results are as Figure 2 shown;

[0057] The determination results show that the alkaloid content in the extract of Phellodendron amurense Rupr. bark obtained by the semi-bionic extraction method is significantly higher than that of the general reflux extraction method, and the transdermal absorption rate is 3.6 times higher than that of the extract obtained by the general extraction method.

[0058] Example 2: Aesculus fruit extract;

[0059] 10 Kg of Aesculus fruits are dried at 60 °C, crushed, and sieved through a 40-mesh sieve;

[0060] Weigh 2.5 Kg of hydrolyzed keratin, 2.5 Kg of hydrolyzed collagen, and 5 Kg of phytoceramide respectively, put them into 200 Kg of water, adjust the pH to 6.2 with acetic acid - sodium acetate buffer solution, and stir at a constant temperature of 55 °C to form a clear and transparent colloidal solution;

[0061] Put 10 Kg of the Aesculus fruit powder into a percolation column, perform percolation extraction with the above 210 Kg of colloidal solution, control the extraction environment temperature at 45 °C, control the percolation flow rate at 0.1 BV / h, collect the percolation extract, concentrate, and dry;

[0062] The above dried percolation extract is heated under reflux with 20 times the amount of 80% ethanol for extraction twice, the extraction solutions are combined, concentrated, and the concentrated paste is purified with D101 macroporous adsorption resin. First, elute with pure water for 10 BV, then elute with 50% ethanol aqueous solution for 20 BV, collect the ethanol aqueous solution elution part, concentrate, and dry to obtain 2.36 Kg of Aesculus fruit extract;

[0063] Use the reflux extraction method commonly used in production to obtain Aesculus fruit extract as the reference group for the transdermal absorption rate test. The extraction method is: the Aesculus fruit powder is heated under reflux with 20 times the amount of 80% ethanol for extraction twice, the extraction solutions are combined, concentrated, and dried;

[0064] Using aescin A as the standard product, use ultraviolet - visible spectrophotometry to determine the content of total triterpenoid saponins in the above Aesculus fruit extract respectively;

[0065] Measure the transdermal absorption rates of the Aesculus fruit extracts obtained by the semi - bionic extraction method and the reflux extraction method, repeat each method 3 times, and the experimental results are as Figure 3 shown;

[0066] The measurement results show that the content of triterpenoid saponins in the extract obtained from Aesculus fruits by the semi - bionic extraction method is significantly higher than that of the general reflux extraction method, and the transdermal absorption rate is 3.125 times higher than that of the extract obtained by the general extraction method;

[0067] Example 3: Gentiana extract

[0068] 10 Kg of the whole Gentiana plant is dried at 60 °C, crushed, and sieved through a 40 - mesh sieve;

[0069] Weigh 5 Kg of hydrolyzed keratin, 3 Kg of hydrolyzed collagen, and 2.5 Kg of phytoceramide separately, put them into 200 Kg of water, adjust the pH to 7.5 with acetic acid - sodium acetate buffer solution, and stir at a constant temperature of 55 °C to form a clear and transparent colloidal solution;

[0070] Put 10 Kg of gentian powder into a percolation column, perform percolation extraction with the above 210.5 Kg of colloidal solution, control the extraction environment temperature at 35 °C, control the percolation flow rate at 0.8 BV / h, collect the percolation extract, concentrate it, and dry it;

[0071] The above - dried percolation extract is reflux - extracted with 10 times the amount of 50% ethanol by heating for 2 times, the extraction liquids are combined, concentrated, and the concentrated paste is purified with D101 macroporous adsorption resin. First, elute with pure water for 2 BV, then elute with 30% ethanol aqueous solution for 5 BV, collect the eluted part of the ethanol aqueous solution, concentrate it, and dry it to obtain 2.87 Kg of gentian extract;

[0072] Use the reflux extraction method commonly used in production to obtain gentian extract as the reference group for the transdermal absorption rate test. The extraction method is: reflux - extract gentian powder with 10 times the amount of 50% ethanol by heating for 2 times, combine the extraction liquids, concentrate them, and dry them;

[0073] Use gentiopicroside as the standard product, and use ultraviolet - visible spectrophotometry to determine the content of total iridoid glycosides in the above - mentioned gentian extract respectively;

[0074] Determine the transdermal absorption rates of the gentian extracts obtained by the semi - bionic extraction method and the reflux extraction method, repeat each method 3 times, and the experimental results are as Figure 4 shown;

[0075] The determination results show that the content of iridoid glycosides in the extract obtained from the whole gentian plant by the semi - bionic extraction method is significantly higher than that of the general reflux extraction method, and the transdermal absorption rate is 2.177 times higher than that of the extract obtained by the general extraction method;

[0076] Example 4: Campsis grandiflora extract;

[0077] Dry 10 Kg of Campsis grandiflora at 60 °C, pulverize it, and sieve it through a 40 - mesh sieve;

[0078] Weigh 4 Kg of hydrolyzed keratin, 4 Kg of hydrolyzed collagen, and 2.5 Kg of phytoceramide separately, put them into 200 Kg of water, adjust the pH to 8.0 with acetic acid - sodium acetate buffer solution, and stir at a constant temperature of 55 °C to form a clear and transparent colloidal solution;

[0079] Put 10 Kg of Campsis grandiflora powder into a percolation column, perform percolation extraction with the above 210.5 Kg of colloidal solution, control the extraction environment temperature at 40 °C, control the percolation flow rate at 0.5 BV / h, collect the percolation extract, concentrate it, and dry it;

[0080] The above-mentioned dried percolation extract was extracted twice by heating under reflux with 20 times its volume of 70% ethanol. The extraction solutions were combined, concentrated, and the concentrated paste was purified using D101 macroporous adsorption resin. First, it was eluted with pure water for 6 BV, and then eluted with a 70% ethanol aqueous solution for 8 BV. The eluate of the ethanol aqueous solution was collected, concentrated, and dried to obtain 1.07 Kg of Campsis grandiflora extract;

[0081] The Campsis grandiflora extract obtained by the commonly used reflux extraction method in production was used as the reference group for the transdermal absorption rate test. The extraction method was as follows: Campsis grandiflora powder was extracted twice by heating under reflux with 20 times its volume of 70% ethanol. The extraction solutions were combined, concentrated, and dried;

[0082] Using rutin as the standard product, the content of total flavonoids in the above-mentioned Campsis grandiflora extract was determined by ultraviolet-visible spectrophotometry;

[0083] The transdermal absorption rates of the Campsis grandiflora extracts obtained by the semi-bionic extraction method and the reflux extraction method were measured, and each method was repeated 3 times. The experimental results were as Figure 5 described;

[0084] The measurement results showed that the flavonoid content in the extract obtained from Campsis grandiflora by the semi-bionic extraction method was significantly higher than that of the general reflux extraction method, and the transdermal absorption rate was 4.542 times higher than that of the extract obtained by the general extraction method.

[0085] Example 5: Anemarrhena asphodeloides rhizome extract;

[0086] 10 Kg of Anemarrhena asphodeloides rhizome was dried at 60 °C, crushed, and passed through a 40-mesh sieve;

[0087] 5 Kg of hydrolyzed keratin, 5 Kg of hydrolyzed collagen, and 2.5 Kg of phytoceramide were weighed respectively, put into 200 Kg of water, and the pH was adjusted to 5.5 with acetic acid-sodium acetate buffer solution. Stirred at a constant temperature of 55 °C to form a clear and transparent colloidal solution;

[0088] 10 Kg of the Anemarrhena asphodeloides rhizome powder was put into a percolation column, and percolation extraction was carried out with the above 212.5 Kg of colloidal solution. The temperature of the extraction environment was controlled at 35 °C, and the percolation flow rate was controlled at 0.2 BV / h. The percolation extraction solution was collected, concentrated, and dried;

[0089] The above-mentioned dried percolation extract was extracted twice by heating under reflux with 20 times its volume of 60% ethanol. The extraction solutions were combined, concentrated, and the concentrated paste was purified using D101 macroporous adsorption resin. First, it was eluted with pure water for 10 BV, and then eluted with a 70% ethanol aqueous solution for 5 BV. The eluate of the ethanol aqueous solution was collected, concentrated, and dried to obtain 3.16 Kg of Anemarrhena asphodeloides rhizome extract;

[0090] The rhizome extract of Anemarrhena asphodeloides was obtained by the reflux extraction method commonly used in production as the reference group for the transdermal absorption rate test. The extraction method was as follows: The rhizome powder of Anemarrhena asphodeloides was refluxed and extracted twice with 20 times the amount of 60% ethanol by heating, the extraction solutions were combined, concentrated, and dried.

[0091] Using timosaponin B2 as the standard, the total saponin content in the above-mentioned rhizome extract of Anemarrhena asphodeloides was determined by ultraviolet-visible spectrophotometry.

[0092] The transdermal absorption rates of the rhizome extracts of Anemarrhena asphodeloides obtained by the semi-bionic extraction method and the reflux extraction method were measured, and each method was repeated 3 times. The experimental results were as Figure 6 described;

[0093] The measurement results showed that the saponin content in the extract obtained from the rhizome of Anemarrhena asphodeloides by the semi-bionic extraction method was significantly higher than that of the general reflux extraction method, and the transdermal absorption rate was 2.806 times higher than that of the extract obtained by the general extraction method.

[0094] Example 6: Eucommia ulmoides leaf extract;

[0095] 10 Kg of Eucommia ulmoides leaves were dried at 60 °C, pulverized, and passed through a 40-mesh sieve.

[0096] Weigh 2.5 Kg of hydrolyzed keratin, 2.5 Kg of hydrolyzed collagen, and 3 Kg of phytoceramide respectively, put them into 200 Kg of water, adjust the pH to 8.5 with acetic acid-sodium acetate buffer solution, and stir at a constant temperature of 55 °C to form a clear and transparent colloidal solution.

[0097] 10 Kg of the Eucommia ulmoides leaf powder was put into a percolation column, and the above 208 Kg of colloidal solution was used for percolation extraction. The extraction environment temperature was controlled at 45 °C, and the percolation flow rate was controlled at 0.8 BV / h. The percolation extract was collected, concentrated, and dried.

[0098] The above-mentioned dried percolation extract was refluxed and extracted twice with 10 times the amount of 50% ethanol by heating. The extraction solutions were combined, concentrated, and the concentrated paste was purified with D101 macroporous adsorption resin. First, it was eluted with pure water for 2 BV, and then eluted with 30% ethanol aqueous solution for 3 BV. The ethanol aqueous solution elution part was collected, concentrated, and dried to obtain 1.07 Kg of Eucommia ulmoides leaf extract.

[0099] The Eucommia ulmoides leaf extract was obtained by the reflux extraction method commonly used in production as the reference group for the transdermal absorption rate test. The extraction method was as follows: The Eucommia ulmoides leaf powder was refluxed and extracted twice with 10 times the amount of 50% ethanol by heating, the extraction solutions were combined, concentrated, and dried.

[0100] Using chlorogenic acid as the standard, the chlorogenic acid content in the above-mentioned Eucommia ulmoides leaf extract was determined by high performance liquid chromatography.

[0101] Determine the transdermal absorption rates of chlorogenic acid extracts obtained by the semi-bionic extraction method and the reflux extraction method, with each method repeated 3 times. The experimental results are as Figure 7 shown;

[0102] The determination results show that the chlorogenic acid content in the extract obtained from Eucommia ulmoides leaves by the semi-bionic extraction method is higher than that of the general reflux extraction method, and the transdermal absorption rate is 0.431 times higher than that of the extract obtained by the general extraction method;

[0103] Furthermore, compared with the general extraction method, the main difference of the present invention is the different extraction solutions selected. The extraction solution selected by the general extraction method is water or organic solvents such as ethanol, methanol, ethyl acetate, petroleum ether, etc.; the solvent used in this patent is a colloidal solution prepared by mixing hydrolyzed keratin, hydrolyzed collagen, and phytoceramide. The characteristic of this solution is that it has a certain selectivity for the extraction target substance and a low solubility for impurities;

[0104] Furthermore, compared with the semi-bionic extraction technology, the bionic method of the present invention has obvious innovation. The reported bionic extraction methods mainly simulate the human gastrointestinal fluid by adding digestive enzymes, adjusting the pH, etc. for bionic extraction in order to improve the digestive absorption rate of the extract. The bionic method in this patent is to dissolve the hydrolyzates and analogs of the important component proteins and lipids of the skin in water, adjust the pH and temperature, and simulate the human skin absorption environment for extraction to improve the skin absorption rate of the extract. The transdermal absorption rate is increased by 40% - 450% compared with the general reflux extraction method;

[0105] Furthermore, compared with the post-treatment of general skin care raw material extraction, the present invention has certain innovation. After obtaining the plant skin care raw material extract, the general method is to simply treat the extract by direct drying, solvent extraction, centrifugation, etc. This patent conducts secondary extraction after obtaining the extract and purifies and enriches it through macroporous resin, which increases the content of the effective components of the extract and further improves the stability and safety of the raw material.

[0106] The embodiments of the present invention have been described in detail above in conjunction with the accompanying drawings. However, the present invention is not limited to the above embodiments. Within the scope of knowledge possessed by those of ordinary skill in the art in the technical field, various changes can be made without departing from the purpose of the present invention.

Claims

1. A method for preparing a semi-bionic extraction plant skin care raw material, characterized in that: The method comprises the following specific steps: S1: preparing a percolation extract: mixing hydrolyzed keratin (1%-5%), hydrolyzed collagen (1%-5%) and phytoceramide (1%-5%) and adding the mixture into water; S2 Plant material pretreatment: The plant material to be used for extraction is dried at 60°C, then crushed, and then passed through a 40-mesh sieve to obtain uniform plant powder; S3 Percolation extraction: The pretreated plant powder is loaded into a percolation column and percolated with a prepared percolation extract at a liquid-to-solid ratio of 10-20:1; S4 secondary ethanol reflux extraction: take the above dried percolation extract, add 10-20 times the amount of 50%-80% ethanol aqueous solution, heat and reflux extraction, extract twice; S5 resin purification and enrichment: D101 macroporous adsorption resin was used to purify the concentrated paste.

2. The method for preparing a semi-bionic extraction plant skin care raw material according to claim 1, characterized in that: The preparation of the S1 percolation extract comprises weighing 1-5 kg ​​of hydrolyzed keratin, 1-5 kg ​​of hydrolyzed collagen, and 1-5 kg ​​of phytoceramide and putting them into 100 kg of water; Use acetic acid-sodium acetate buffer to adjust the pH to 5.5-8.5, and stir at a constant temperature of 55°C until a clear and transparent colloidal solution is formed; Among them, this colloidal solution is used as a percolation extract, using the hydrolyzate of important components of the skin to simulate the human skin environment, providing key conditions for the subsequent selective extraction of active substances with strong affinity to the skin.

3. The method for preparing a semi-bionic extraction plant skin care raw material according to claim 1, characterized in that: The S3 percolation extraction controls the pH value during the extraction process to be 5.5-8.5, the extraction temperature to be 35-45° C., and the percolation flow rate to be 0.1-1 BV / h (0.1-1 column volume of percolation liquid flows out per hour); Among them, under such bionic conditions, hydrogen bond adsorption, intermolecular force and ion adsorption are used to selectively extract active ingredients with strong affinity to the skin into the percolation extract, and the extract is collected and concentrated and dried to obtain a preliminary percolation extract.

4. The method for preparing a semi-bionic extraction plant skin care raw material according to claim 1, characterized in that: The S4 secondary ethanol reflux extraction further extracts the effective ingredients in the percolation extract, thereby improving the purity and active substance content of the extract. The two extracts are then combined and concentrated to obtain a concentrated paste.

5. The method for preparing a semi-bionic extraction plant skin care raw material according to claim 1, characterized in that: The S5 resin is purified and enriched by first eluting with pure water to remove impurities in the extract; then eluting with 30%-100% ethanol aqueous solution, and the effective ingredients will be eluted in this process; The eluted part of the ethanol aqueous solution is collected, concentrated and dried again, and finally a semi-bionic extraction plant skin care raw material extract with a high transdermal absorption rate is obtained.

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