Method for preparing biofuel based on microbial decomposition inoculant

Abstract

The invention discloses a method for preparing biofuel based on a microbial decomposition inoculant. According to the method, in the process of preparing the biofuel, the decomposition accelerant is added, so that fatty acid in a cracking product is effectively removed while cracking is performed in the subsequent preparation process, and meanwhile, the heavy component is remarkably reduced, so that less waste is generated; the method has the advantages that the prepared biomass oil is stable in quality, and the yield of the prepared biofuel is increased, so that a large amount of waste livestock and poultry manure can be recycled, and the environmental protection property of the method is improved. Before the biofuel is prepared, waste livestock and poultry manure is subjected to acid treatment, metal impurities in waste livestock and poultry manure raw oil form complexes, the complexes are brought out through hydration treatment, finally, soap substances are adsorbed firstly through adsorption of an adsorbent, and phospholipid is removed, so that the impurities in the biofuel are reduced as much as possible, and the biofuel is prepared. And the purity of the whole biofuel is increased.

Description

Technical Field

[0001] The present invention belongs to the technical field of biofuels, and particularly relates to a method for preparing biofuels based on microbial decomposition agents. Background Art

[0002] With the improvement of China's market economy, in order to meet the production and living needs of people, large-scale breeding industries have developed rapidly. However, a large amount of livestock and poultry manure produced has not been reasonably disposed of and utilized in a timely manner, becoming a major problem in the treatment of agricultural non-point source pollution in China. According to investigations, China's livestock and poultry breeding industries produce 3.8 billion tons of livestock and poultry manure every year, but the effective treatment rate is less than 50%. When livestock and poultry manure is decomposed by the microorganisms it contains, a large amount of greenhouse gases will be produced, posing a great threat to animals, plants, and human health. In recent years, the research on the fertilizerization of livestock and poultry manure has attracted the attention of scholars at home and abroad. In the process of fertilizer production, related research on deodorization and nitrogen preservation, as well as the harmless treatment of antibiotics, heavy metals (especially Cu, Zn, As), and ARGs, has become a hot topic. A large number of studies have shown that co-fermentation is beneficial to reducing the ecological risks of anaerobic fermentation products of livestock and poultry manure. The biosurfactant sophorolipid (SLs) is the cheapest biosurfactant on the market and is biodegradable. Existing research has shown that SLs can promote the reduction of antibiotics and ARGs in the soil. Therefore, the biosurfactant sophorolipid (SLs) can be used to prepare biofuels.

[0003] However, the oil production efficiency of common methods for preparing biofuels using waste livestock and poultry manure is relatively low, and the purity of the prepared fuel is not high enough. Summary of the Invention

[0004] The purpose of the present invention is to provide a method for preparing biofuels based on microbial decomposition agents in order to solve the above-mentioned problems.

[0005] The technical solution adopted by the present invention is as follows: A method for preparing biofuels based on microbial decomposition agents, the method for preparing biofuels based on microbial decomposition agents includes the following steps: S1: First, purify the raw materials for preparing biofuels based on microbial decomposition agents. Heat the waste livestock and poultry manure to 65°C - 75°C, add an aqueous solution of acid with a concentration of 80 wt.% to the waste livestock and poultry manure for acid treatment, control the temperature at 75°C, and the acid treatment time is 50 min; S2: Add water to the mixture obtained in step S1, control the temperature at 60°C - 80°C, and carry out hydration treatment. The hydration treatment time is 30 - 60 min; The water accounts for 1% - 30% of the mass of the waste livestock and poultry manure; S3: After the hydration treatment in step S2 is completed, the material temperature is controlled to be 60°C to 80°C and allowed to stand, and the material temperature is maintained at 60°C to 80°C for liquid separation, and the waste water is removed after oil-water separation, and a physical treatment agent is added to the oil phase after oil-water separation for adsorption, and the temperature is controlled to be 80°C to 120°C, and mixed evenly; the physical treatment agent accounts for 1% to 10% of the mass of the waste livestock and poultry manure; S4: Control the material temperature to 80℃~120℃ to separate the physical treatment agent and remove the physical treatment agent; vacuum dry the oil after the physical treatment agent adsorbs, the drying temperature is 110℃~150℃, the vacuum pressure is -0.02MPa~-0.10MPa; dry to a water content of ≤0.5Wt.%, and obtain the pre-treated decontaminated oil; S5: preparing a microbial decomposition promoter, mixing at least one of sophorolipids, waste fungus bran, Triton and sodium dodecylbenzene sulfonate with oleate in a mass ratio of 1-3:14-17 to obtain a decomposition promoter; S6: 1000-1200 parts by weight of the degreased oil prepared in step S4 and 100-140 parts by weight of the decomposition promoter prepared in step S5 are weighed and mixed to obtain a mixture; S7: mixing the mixture prepared in step S6 with hydrogen to carry out a hydrogenation reaction in the presence of a hydrogenation catalyst; S8: subjecting the material obtained in step S7 to high pressure separation to obtain gas, product oil and water; the gas circulates in the reaction device; S9: separating the product oil and water obtained in step S8 at low pressure, and performing steam stripping and desulfurization on the product oil separated at low pressure; S10: the desulfurized material in step S9 is cracked at 450-600 degrees Celsius and a nitrogen flow rate of 70-90 mL / min for 8-12 minutes to obtain biofuel; S11: The biofuel produced in step S10 is collected and stored, thereby completing the entire production process of biofuel produced from waste livestock and poultry manure.

[0006] In a preferred embodiment, in step S1, the acid aqueous solution accounts for 1% to 15% of the mass of the waste livestock and poultry manure.

[0007] In a preferred embodiment, in step S1, the waste livestock and poultry manure is selected from one or more of waste cow manure, waste chicken manure, waste duck manure or waste pig manure.

[0008] In a preferred embodiment, in step S1, the organic acid or inorganic acid is phosphoric acid, citric acid, sulfuric acid or oxalic acid.

[0009] In a preferred embodiment, in the step S3, the physical treatment agent is selected from one or more of silica gel, activated carbon, activated clay, natural bleaching earth, zeolite, attapulgite, diatomite, activated alumina, and alumina treated with sulfurous acid.

[0010] In a preferred embodiment, in the step S6, before the waste livestock and poultry manure is mixed with the decomposition promoter, dehydration of the waste livestock and poultry manure is also included; the dehydration is carried out by heating the waste livestock and poultry manure to 100-120 °C, and under the condition of vacuum pumping, dehydrating treatment with anhydrous calcium chloride for 20-26 hours and then filtering and separating.

[0011] In a preferred embodiment, in the step S7, the hydrogenation catalyst is composed of an active component with a content of 1 wt% - 30 wt% and a catalyst carrier; the active component is two or three of nickel metal salt, molybdenum metal salt, cobalt metal salt, and tungsten metal salt; the catalyst carrier is a modified molecular sieve - alumina.

[0012] In a preferred embodiment, in the step S7, for the hydrogenation reaction, the reaction conditions are: liquid hourly space velocity 0.5 - 2 h-1, hydrogen-oil volume ratio 500:1 - 2000:1, reaction pressure 4 - 8 MPa, and reaction temperature 320 - 380 °C.

[0013] In a preferred embodiment, the catalyst carrier includes molecular sieve, carbon material, oxide, and amorphous silica-alumina; the metal center includes Group VIII and / or Group VIIB metals.

[0014] In a preferred embodiment, in the step S10, a tubular cracking furnace is used to crack the microemulsified waste livestock and poultry manure.

[0015] In summary, due to the adoption of the above technical solutions, the beneficial effects of the present invention are as follows: 1. In the present invention, during the preparation of biofuel, by adding a decomposition promoter, during the subsequent preparation process, fatty acids in the cracking products are effectively removed while cracking, and at the same time, the recombination component is significantly reduced, so that less waste is generated, the quality of the prepared bio-oil is stable, and the yield of the prepared biofuel is also improved. Therefore, a large amount of waste livestock and poultry manure can be reused, thereby increasing the environmental protection of the present invention.

[0016] 2. In the present invention, before preparing the biofuel, the waste livestock and poultry manure is subjected to acid treatment. The metal impurities in the raw material oil of the waste livestock and poultry manure form complexes and are removed through hydration treatment. Finally, the adsorbent adsorbs the saponaceous substances first and removes the phospholipids attached thereto. While removing these two types of substances, the processing pigments, metal ion derivatives, and other elements and impurities are also removed, thereby minimizing the impurities inside the biofuel as much as possible during the subsequent preparation process and increasing the purity of the overall biofuel. Embodiment

[0017] To make the objectives, technical solutions, and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the embodiments of the present invention. Obviously, the described embodiments are part of the embodiments of the present invention, rather than all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without making creative efforts fall within the scope of protection of the present invention. Example

[0018] A method for preparing biofuel based on a microbial decomposition agent, the method for preparing biofuel based on a microbial decomposition agent comprising the following steps: S1: First, purify the raw material for preparing biofuel based on a microbial decomposition agent. Heat the waste livestock and poultry manure to 65°C - 75°C, add an aqueous solution of acid with a concentration of 80 wt.% to the waste livestock and poultry manure for acid treatment, control the temperature at 75°C, and the acid treatment time is 50 min; S2: Add water to the mixture obtained in step S1, control the temperature at 60°C - 80°C, and perform hydration treatment. The hydration treatment time is 30 - 60 min; the water accounts for 1% - 30% of the mass of the waste livestock and poultry manure; S3: After the hydration treatment in step S2 is completed, control the material temperature at 60°C - 80°C and let it stand. Keep the material temperature at 60°C - 80°C for liquid separation. After oil-water separation, remove the wastewater. Add a physical treatment agent to the oil phase after oil-water separation for adsorption, control the temperature at 80°C - 120°C, and mix evenly; the physical treatment agent accounts for 1% - 10% of the mass of the waste livestock and poultry manure; S4: Control the material temperature at 80°C - 120°C to separate the physical treatment agent and remove the physical treatment agent; the oil and fat after adsorption by the physical treatment agent is subjected to vacuum drying. The drying temperature is 110°C - 150°C, and the vacuum pressure is -0.02 MPa - -0.10 MPa; dry until the water content ≤ 0.5 Wt.%, and obtain the pretreated decontaminated oil and fat; S5: Prepare a microbial decomposition promoter by mixing at least one of sophorolipid, waste mushroom residue, Triton, and sodium dodecylbenzenesulfonate with oleate in a mass ratio of 1-3:14-17 to obtain a decomposition promoter; S6: Weigh 1000 parts by weight of the decontaminated oil obtained in step S4 and 100 parts by weight of the decomposition promoter obtained in step S5, and fully mix them to obtain a mixture; S7: Mix the mixture obtained in step S6 with hydrogen and carry out a hydrogenation reaction in the presence of a hydrogenation catalyst; S8: Carry out high-pressure separation on the material obtained in step S7 to obtain gas, product oil, and water; the gas is recycled in the reaction device; S9: Carry out low-pressure separation on the product oil and water obtained in step S8, and subject the product oil separated under low pressure to steam stripping desulfurization; S10: Pyrolyze the desulfurized material in step S9 at 450-600 °C for 8-12 min under the condition that the nitrogen flow rate is 70-90 mL / min to obtain biofuel; S11: Collect and store the biofuel obtained in step S10, and the entire production process of producing biofuel from waste livestock and poultry manure can be ended.

[0019] In the said step S1, the aqueous solution of the acid accounts for 1% - 15% of the mass of the waste livestock and poultry manure.

[0020] In the said step S1, the waste livestock and poultry manure is selected from one or more of waste cow manure, waste chicken manure, waste duck manure, or waste pig manure.

[0021] In the said step S1, the organic acid or inorganic acid is phosphoric acid, citric acid, sulfuric acid, or oxalic acid.

[0022] In the said step S3, the physical treatment agent is selected from one or more of silica gel, activated carbon, activated clay, natural bleaching clay, zeolite, attapulgite, diatomite, activated alumina, and alumina treated with sulfurous acid.

[0023] In the said step S6, before mixing the waste livestock and poultry manure with the decomposition promoter, dehydration of the waste livestock and poultry manure is also included; the dehydration is to heat the waste livestock and poultry manure to 100-120 °C, and under the condition of vacuum pumping, carry out dehydration treatment with anhydrous calcium chloride for 20-26 hours and then filter and separate.

[0024] In the said step S7, the hydrogenation catalyst consists of an active component with a content of 1 wt% - 30 wt% and a catalyst carrier; the active component is two or three of nickel metal salt, molybdenum metal salt, cobalt metal salt, and tungsten metal salt; the catalyst carrier is a modified molecular sieve - alumina.

[0025] In the step S7, the hydrogenation reaction is carried out under the following reaction conditions: liquid hourly space velocity of 0.5 - 2 h-1, hydrogen-oil volume ratio of 500:1 - 2000:1, reaction pressure of 4 - 8 MPa, and reaction temperature of 320 - 380 °C.

[0026] The catalyst support includes molecular sieve, carbon material, oxide, and amorphous silica-alumina; the metal center includes Group VIII and / or Group VIIB metals.

[0027] In the step S10, a tubular pyrolysis furnace is used to pyrolyze the microemulsified waste livestock and poultry manure.

[0028] In the present invention, during the preparation of biofuel, by adding a decomposition promoter, fatty acids in the pyrolysis products are effectively removed during pyrolysis in the subsequent preparation process, and the recombination components are significantly reduced, resulting in less waste, stable quality of the prepared bio-oil, and increased yield of the prepared biofuel. Therefore, a large amount of waste livestock and poultry manure can be reused, thereby enhancing the environmental protection of the present invention.

[0029] In the present invention, before preparing biofuel, the waste livestock and poultry manure is acid-treated. Metal impurities in the waste livestock and poultry manure raw material oil form complexes and are removed through hydration treatment. Finally, an adsorbent is used for adsorption. First, saponins are adsorbed, and phospholipids are removed along with them. When removing these two types of substances, processing pigments, metal ion derivatives, and other elements and impurities are also removed, thereby minimizing impurities in the biofuel during the subsequent preparation process and increasing the purity of the overall biofuel. Example

[0030] A method for preparing biofuel based on a microbial decomposition agent, the method for preparing biofuel based on a microbial decomposition agent includes the following steps: S1: First, purify the raw material for preparing biofuel based on a microbial decomposition agent. Heat the waste livestock and poultry manure to 65 °C - 75 °C, add an aqueous solution of acid with a concentration of 80 wt.% to the waste livestock and poultry manure for acid treatment, control the temperature at 75 °C, and the acid treatment time is 50 min; S2: Add water to the mixture obtained in step S1, control the temperature at 60 °C - 80 °C, and carry out hydration treatment. The hydration treatment time is 30 - 60 min; the water accounts for 1% - 30% of the mass of the waste livestock and poultry manure; S3: After the hydration treatment in step S2 is completed, control the material temperature at 60 °C - 80 °C and let it stand. Keep the material temperature at 60 °C - 80 °C for liquid separation. After oil-water separation, remove the wastewater. Add a physical treatment agent for adsorption to the oil phase after oil-water separation, control the temperature at 80 °C - 120 °C, and mix evenly; the physical treatment agent accounts for 1% - 10% of the mass of the waste livestock and poultry manure; S4: Control the material temperature at 80°C - 120°C for physical treatment agent separation to remove the physical treatment agent; the oil and fat adsorbed by the physical treatment agent are subjected to vacuum drying, with the drying temperature being 110°C - 150°C and the vacuum pressure being -0.02 MPa - -0.10 MPa; dry until the water content ≤ 0.5 Wt.%, obtaining the impurity-removed oil and fat after pretreatment. S5: Prepare the microbial decomposition promoter. Mix at least one of sophorolipid, waste mushroom bran, Triton, and sodium dodecylbenzenesulfonate with oleate in a mass ratio of 1 - 3:14 - 17 to obtain the decomposition promoter. S6: Weigh 1200 parts by weight of the impurity-removed oil and fat prepared in step S4 and 140 parts by weight of the decomposition promoter prepared in step S5, and fully mix them to obtain a mixture. S7: Mix the mixture prepared in step S6 with hydrogen and carry out a hydrogenation reaction in the presence of a hydrogenation catalyst. S8: Carry out high-pressure separation on the material prepared in step S7 to obtain gas, product oil, and water; the gas is recycled in the reaction device. S9: Carry out low-pressure separation on the product oil and water prepared in step S8, and subject the product oil separated at low pressure to steam stripping desulfurization. S10: Pyrolyze the desulfurized material in step S9 at 450 - 600 °C for 8 - 12 min under the condition that the nitrogen flow rate is 70 - 90 mL / min to obtain biofuel. S11: Collect and store the biofuel prepared in step S10, and the entire production process of producing biofuel from waste livestock and poultry manure can be ended.

[0031] In the said step S1, the aqueous solution of the acid accounts for 1% - 15% of the mass of the waste livestock and poultry manure.

[0032] In the said step S1, the waste livestock and poultry manure is selected from one or more of waste cow dung, waste chicken dung, waste duck dung, or waste pig dung.

[0033] In the said step S1, the organic acid or inorganic acid is phosphoric acid, citric acid, sulfuric acid, or oxalic acid.

[0034] In the said step S3, the physical treatment agent is selected from one or more of silica gel, activated carbon, activated clay, natural bleaching earth, zeolite, attapulgite, diatomite, activated alumina, and alumina treated with sulfurous acid.

[0035] In the said step S6, before mixing the waste livestock and poultry manure with the decomposition promoter, it also includes dehydrating the waste livestock and poultry manure; the dehydration is to heat the waste livestock and poultry manure to 100 - 120 °C, and under the condition of vacuum pumping, carry out dehydration treatment with anhydrous calcium chloride for 20 - 26 hours and then filter and separate.

[0036] In step S7, the hydrogenation catalyst is composed of an active component with a content of 1 wt% - 30 wt% and a catalyst carrier; the active component is two or three of nickel metal salt, molybdenum metal salt, cobalt metal salt, and tungsten metal salt; the catalyst carrier is a modified molecular sieve - alumina.

[0037] In step S7, for the hydrogenation reaction, the reaction conditions are as follows: liquid hourly space velocity 0.5 - 2 h-1, hydrogen-oil volume ratio 500:1 - 2000:1, reaction pressure 4 - 8 MPa, and reaction temperature 320 - 380 °C.

[0038] The catalyst carrier includes molecular sieve, carbon material, oxide, and amorphous silica-alumina; the metal center includes Group VIII and / or Group VIIB metals.

[0039] In step S10, a tubular cracking furnace is used to crack the microemulsified waste livestock and poultry manure.

[0040] In the present invention, during the preparation of biofuel, by adding a decomposition promoter, fatty acids in the cracking products are effectively removed during cracking in the subsequent preparation process, and the recombinant components are significantly reduced, resulting in less waste. This makes the quality of the produced bio-oil stable and also improves the yield of the prepared biofuel. Therefore, a large amount of waste livestock and poultry manure can be reused, thereby increasing the environmental friendliness of the present invention.

[0041] In the present invention, before preparing biofuel, the waste livestock and poultry manure is acid-treated. Metal impurities in the waste livestock and poultry manure raw oil form complexes and are removed through hydration treatment. Finally, the adsorbent first adsorbs saponins and phospholipids are also removed. While removing these two types of substances, processing pigments, metal ion derivatives, and other elements and impurities are also removed, thereby minimizing the impurities in the biofuel during the subsequent preparation process and increasing the purity of the overall biofuel.

[0042] It should be noted that in this text, relational terms such as first and second are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any actual relationship or order between these entities or operations. Moreover, the term "comprising", "including" or any other variation thereof is intended to cover non-exclusive inclusion, so that a process, method, article or device comprising a series of elements not only includes those elements, but also includes other elements not expressly listed, or elements inherent to such process, method, article or device. Without further limitation, an element defined by the statement "comprising an..." does not exclude the presence of additional identical elements in the process, method, article or device comprising said element.

[0043] The above embodiments are only used to illustrate the technical solutions of the present invention and are not intended to limit them; although the present invention has been described in detail with reference to the foregoing embodiments, those of ordinary skill in the art should understand that they can still modify the technical solutions described in the foregoing embodiments, or perform equivalent replacements for some of the technical features; and these modifications or replacements do not cause the essence of the corresponding technical solutions to deviate from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims

1. A method for preparing biofuel based on microbial decomposition agent, characterized in that: The method for preparing biofuel based on microbial decomposition agent comprises the following steps: S1: First, the raw materials for preparing biofuels based on microbial decomposition agents are purified, the waste livestock and poultry manure is heated to 65°C to 75°C, and an aqueous solution with a concentration of 80wt.% acid is added to the waste livestock and poultry manure for acid treatment, the temperature is controlled at 75°C, and the acid treatment time is 50min; S2: adding water to the mixture obtained in step S1, controlling the temperature at 60°C to 80°C, and performing hydration treatment for 30 to 60 minutes; the water accounts for 1% to 30% of the mass of the waste livestock and poultry manure; S3: After the hydration treatment in step S2 is completed, the material temperature is controlled to be 60°C to 80°C and allowed to stand, and the material temperature is maintained at 60°C to 80°C for liquid separation, and the waste water is removed after oil-water separation, and a physical treatment agent is added to the oil phase after oil-water separation for adsorption, and the temperature is controlled to be 80°C to 120°C, and mixed evenly; the physical treatment agent accounts for 1% to 10% of the mass of the waste livestock and poultry manure; S4: Control the material temperature to 80℃~120℃ to separate the physical treatment agent and remove the physical treatment agent; vacuum dry the oil after the physical treatment agent adsorbs, the drying temperature is 110℃~150℃, the vacuum pressure is -0.02MPa~-0.10MPa; dry to a water content of ≤0.5Wt.%, and obtain the pre-treated decontaminated oil; S5: preparing a microbial decomposition promoter, mixing at least one of sophorolipids, waste fungus bran, Triton and sodium dodecylbenzene sulfonate with oleate in a mass ratio of 1-3:14-17 to obtain a decomposition promoter; S6: 1000-1200 parts by weight of the degreased oil prepared in step S4 and 100-140 parts by weight of the decomposition promoter prepared in step S5 are weighed and mixed to obtain a mixture; S7: mixing the mixture prepared in step S6 with hydrogen to carry out a hydrogenation reaction in the presence of a hydrogenation catalyst; S8: subjecting the material obtained in step S7 to high pressure separation to obtain gas, product oil and water; the gas circulates in the reaction device; S9: separating the product oil and water obtained in step S8 at low pressure, and performing steam stripping and desulfurization on the product oil separated at low pressure; S10: the desulfurized material in step S9 is cracked at 450-600 degrees Celsius and a nitrogen flow rate of 70-90 mL / min for 8-12 minutes to obtain biofuel; S11: The biofuel produced in step S10 is collected and stored, thereby completing the entire production process of biofuel produced from waste livestock and poultry manure.

2. The method for preparing biofuel based on microbial decomposition agent according to claim 1, characterized in that: In the step S1, the acid aqueous solution accounts for 1% to 15% of the mass of the waste livestock and poultry manure.

3. The method for preparing biofuel based on microbial decay agent according to claim 1, characterized in that: In step S1, the waste livestock and poultry manure is selected from one or more of waste cow manure, waste chicken manure, waste duck manure or waste pig manure.

4. The method for preparing biofuel based on microbial decay agent as claimed in claim 1, wherein: In step S1, the organic acid or inorganic acid is phosphoric acid, citric acid, sulfuric acid or oxalic acid.

5. The method for preparing biofuel based on microbial decay agent as claimed in claim 1, wherein: In step S3, the physical treatment agent is selected from one or more of silica gel, activated carbon, activated clay, natural bleaching earth, zeolite, attapulgite, diatomaceous earth, activated alumina, and alumina treated with sulfurous acid.

6. The method for preparing biofuel based on microbial decomposition agent as claimed in claim 1, wherein: In the step S6, before the waste livestock and poultry manure is mixed with the decomposition promoter, dehydration of the waste livestock and poultry manure is also included; dehydration is to heat the waste livestock and poultry manure to 100-120 °C, and under the condition of vacuum pumping, dehydrate it with anhydrous calcium chloride for 20-26 hours and then filter and separate.

7. The method for preparing biofuel based on microbial decomposition agent according to claim 1, characterized in that: In the step S7, the hydrogenation catalyst is composed of an active component with a content of 1 wt% - 30 wt% and a catalyst carrier; the active component is two or three of nickel metal salt, molybdenum metal salt, cobalt metal salt, and tungsten metal salt; the catalyst carrier is a modified molecular sieve - alumina.

8. The method for preparing biofuel based on microbial decay agent as claimed in claim 1, wherein: In the step S7, for the hydrogenation reaction, the reaction conditions are: liquid hourly space velocity 0.5~2 h-1, hydrogen-oil volume ratio 500:1~2000:1, reaction pressure 4~8 MPa, and reaction temperature 320 - 380 °C.

9. The method for preparing biofuel based on microbial decay agent according to claim 7, characterized in that: The catalyst carrier includes molecular sieve, carbon material, oxide, and amorphous silica-aluminum; the metal center includes Group VIII and / or Group VIIB metals.

10. A method for preparing biofuel based on microbial decomposition agent, as claimed in claim 1, characterized in that: In the step S10, a tubular pyrolysis furnace is used to pyrolyze the microemulsified waste livestock and poultry manure.