Bacterial water sample collection device and method
By combining a depth judgment system with a pressure sensor and a microcontroller in a bacterial water sample collection device, the problem that existing devices cannot achieve sterile operation and fixed depth triggering at the same time is solved, and high-precision sterile sampling is achieved, enhancing portability and environmental adaptability.
Patent Information
- Application Number
- CN202510194965.7
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-02-21
- Publication Date
- 2025-06-24
AI Technical Summary
The existing bacterial water sample collection device cannot achieve sterile operation and fixed depth triggering at the same time, the quantitative sampling accuracy is low, the portability is poor, and the environmental adaptability is insufficient.
A depth judgment system combined with a pressure sensor and a microcontroller is adopted to collect water pressure data through the pressure sensor. The microcontroller judges the underwater depth based on the data and sends sampling instructions. The actuator performs sterile sampling. The device design includes a double-layer sealed hatch, a folding lightweight structure and a self-cleaning system for enhanced sterility and environmental adaptability.
The sterile sampling depth and sampling accuracy of the acquisition device are improved, the control ability of sampling depth is enhanced, sampling efficiency and data reliability are improved, costs are reduced, and the adaptability of the system is improved.
Smart Images
Figure CN120194975A_ABST
Abstract
Description
Background Art
[0002] Most of the existing bacterial water sample collection devices adopt the following technologies:
[0003] Gravity sampler: It relies on gravity to sink, requires manual control of the sampling depth, and is easily affected by water flow interference, resulting in positioning deviation.
[0004] Mechanically triggered sampler: It is opened at a specific depth through a mechanical structure (such as a spring or magnetic attraction), but the opening time cannot be precisely controlled, and there is a risk of contamination.
[0005] Electric sampler: It relies on battery power, has a complex structure, poor waterproof performance, and is prone to failure in deep water or low-temperature environments.
[0006] The defects and deficiencies of the existing technologies are as follows:
[0007] Inability to balance sterility and depth control: Existing devices are difficult to achieve sterile operation and fixed-depth triggering simultaneously during the sampling process (for example, mechanical triggering requires manual intervention, which may introduce contamination).
[0008] Low quantitative sampling accuracy: Traditional samplers rely on volume estimation, with a high error rate, especially significant deviation in small-volume sampling (such as less than 100 mL).
[0009] Poor portability: Most existing devices adopt rigid structures, with large volume and high weight, and are difficult to adapt to complex field terrains (such as mountains and swamps).
[0010] Insufficient environmental adaptability: Deep water pressure, turbulent impact, or low-temperature environment can easily cause the device's seal to fail or the triggering mechanism to jam. Summary of the Invention
[0011] This application provides a bacterial water sample collection device and method to improve the sterile sampling depth and sampling accuracy of the collection device.
[0012] In a first aspect, a bacterial water sample collection device is provided, including:
[0013] A pressure sensor for collecting water pressure and sending the collected pressure value to the microcontroller;
[0014] The microcontroller for judging the underwater depth according to the received pressure value and sending a sampling instruction to the actuator according to the depth value;
[0015] The actuator for collecting water samples.
[0016] In the above technical solution, a pressure sensor is provided for collecting water pressure and sending the collected pressure value to the microcontroller; the microcontroller is used to judge the underwater depth according to the received pressure value and send a sampling instruction to the actuator according to the depth value; the actuator is used to collect water samples; the aseptic sampling depth and sampling accuracy of the collection device are improved.
[0017] In a specific feasible implementation, the pressure sensor, the microcontroller and the actuator are integrally integrated.
[0018] In a specific feasible implementation, a housing is further included, and the pressure sensor, the microcontroller and the actuator are arranged in the housing, wherein,
[0019] A double-layer sealed hatch is provided on the housing.
[0020] In a specific feasible implementation, the double-layer sealed hatch includes a sterile membrane on the inner layer and a pressure-resistant hatch cover on the outer layer.
[0021] In a specific feasible implementation, the housing includes a frame and a folding layer, wherein,
[0022] The frame is arranged inside the folding layer,
[0023] The frame is connected to the folding layer for folding the folding layer.
[0024] In a specific feasible implementation, the frame includes a lightweight titanium alloy frame.
[0025] In a specific feasible implementation, the folding layer includes a flexible silicone folding layer.
[0026] In a specific feasible implementation, a self-cleaning system is arranged inside the housing for sterilization.
[0027] In a specific feasible implementation, a hydrophobic coating is provided on the surface of the housing for reducing the turbulent resistance.
[0028] In a second aspect, a method for collecting bacterial water samples is provided, including the following steps:
[0029] Using a pressure sensor to collect water pressure and sending the collected pressure value to the microcontroller;
[0030] Using the microcontroller to judge the underwater depth according to the received pressure value and sending a sampling instruction to the actuator according to the depth value;
[0031] Using the actuator to collect water samples.
[0032] In the above technical solution, a pressure sensor is provided to collect water pressure and send the collected pressure value to the microcontroller; the microcontroller is used to judge the underwater depth according to the received pressure value and send a sampling instruction to the actuator according to the depth value; the actuator is used to collect water samples; this improves the aseptic sampling depth and sampling accuracy of the collection device. BRIEF DESCRIPTION OF THE DRAWINGS
[0033] Figure 1 FIG. 6 is a schematic structural diagram (working state) of the bacterial water sample collection device provided by the embodiment of the present application;
[0034] Figure 2 FIG. 10 is a schematic structural diagram (folded state) of the bacterial water sample collection device provided by the embodiment of the present application;
[0035] Figure 3 FIG. 14 is an electrical block diagram of the bacterial water sample collection device provided by the embodiment of the present application;
[0036] Figure 4 FIG. 18 is a flowchart of the bacterial water sample collection method provided by the embodiment of the present application.
[0037] Wherein, 1 - pressure sensor, 2 - microcontroller, 3 - aseptic membrane, 4 - pressure-resistant hatch cover, 5 - frame, 6 - folding layer, 7 - ultraviolet lamp, 8 - airbag, 9 - precision cylinder, 10 - micro motor, 11 - telescopic rod, 12 - counterweight. DETAILED DESCRIPTION OF THE EMBODIMENTS
[0038] The present application will be further described in detail below with reference to the drawings and embodiments. Through these descriptions, the features and advantages of the present application will become more clearly defined.
[0039] The special term "exemplary" here means "serving as an example, embodiment or illustration". Any embodiment described as "exemplary" here does not have to be construed as superior or better than other embodiments. Although various aspects of the embodiments are shown in the drawings, the drawings do not have to be drawn to scale unless otherwise specified.
[0040] In addition, the technical features involved in different embodiments of the present application described below can be combined with each other as long as they do not conflict with each other.
[0041] To facilitate the understanding of the bacterial water sample collection device and method provided by the embodiments of the present application, the application scenario thereof will be described first. The bacterial water sample collection device and method provided by the embodiments of the present application are used to improve the aseptic sampling depth and sampling accuracy of the collection device. The defects and deficiencies of the prior art are as follows: It is impossible to balance asepsis and depth control: It is difficult for the existing devices to achieve aseptic operation and fixed-depth triggering simultaneously during the sampling process (for example, mechanical triggering requires manual intervention, which may introduce contamination). The quantitative sampling accuracy is low: Traditional samplers rely on volume estimation, with a high error rate, especially significant deviation in small-volume sampling (such as less than 100 mL). Poor portability: Most of the existing devices adopt rigid structures, with large volume and high weight, making it difficult to adapt to complex field terrains (such as mountains and swamps). Insufficient environmental adaptability: Deep water pressure, turbulent impact or low-temperature environment can easily cause the device seal to fail or the triggering mechanism to get stuck. Therefore, the embodiments of the present application provide a bacterial water sample collection device and method to improve the aseptic sampling depth and sampling accuracy of the collection device. The following will be described in detail with specific drawings by way of examples.
[0042] Reference Figures 1 to 4 , Figure 1 is a schematic structural diagram (working state) of the bacterial water sample collection device provided by the embodiments of the present application; Figure 2 is a schematic structural diagram (folded state) of the bacterial water sample collection device provided by the embodiments of the present application; Figure 3 is an electrical block diagram of the bacterial water sample collection device provided by the embodiments of the present application; Figure 4 is a flowchart of the bacterial water sample collection method provided by the embodiments of the present application.
[0043] In Figures 1 to 3 , the embodiments of the present application provide a bacterial water sample collection device, including:
[0044] A pressure sensor 1, configured to collect water pressure and send the collected pressure value to a microcontroller 2;
[0045] The microcontroller is configured to judge the underwater depth according to the received pressure value and send a sampling instruction to an actuator according to the depth value;
[0046] The actuator is configured to collect water samples.
[0047] In the above technical solution, by setting a pressure sensor to collect water pressure and send the collected pressure value to a microcontroller; the microcontroller is configured to judge the underwater depth according to the received pressure value and send a sampling instruction to an actuator according to the depth value; the actuator is configured to collect water samples; the aseptic sampling depth and sampling accuracy of the collection device are improved.
[0048] Specifically, by setting up a pressure sensor, a microcontroller, and an actuator for underwater water sample collection, the beneficial effects include:
[0049] Improve sampling accuracy: The pressure sensor can accurately collect water pressure data, and there is a clear corresponding relationship between these data and the underwater depth. The microcontroller can use this relationship to accurately calculate the current underwater depth. Based on the accurate underwater depth information, the actuator can collect water samples at the specified depth, thus greatly improving the sampling accuracy.
[0050] Achieve aseptic sampling: Through the automated control system, human intervention is reduced, and the pollution risk is lowered. The system can be preset to sample at a specific depth, avoiding pollution introduced due to improper human operation.
[0051] Enhance the sampling depth control ability: The pressure sensor monitors the water pressure in real time and provides real-time depth information to the microcontroller. The microcontroller sends sampling instructions to the actuator according to the preset depth range or specific depth value. This real-time and accurate depth control ability enables the system to accurately find and collect water samples at the target depth in a complex and changing underwater environment.
[0052] Improve sampling efficiency: The automated control system can quickly respond to the instructions of the microcontroller and perform sampling operations. Without manual operation, it greatly saves sampling time. At the same time, the system can also preset multiple sampling points and perform sampling in sequence, further improving the sampling efficiency.
[0053] Reduce costs: Although the initial investment may be relatively high, in the long run, the automated sampling system can reduce labor costs. Due to high sampling accuracy and efficiency, the possibility of repeated sampling and ineffective sampling is reduced, thereby lowering the overall cost.
[0054] Enhance data reliability: The data collected automatically has higher reliability and consistency. It avoids errors and biases caused by human factors. It provides more accurate and reliable basic data for subsequent data analysis and scientific research.
[0055] Enhance system adaptability: The system can preset various sampling modes, such as timed sampling, depth-fixed sampling, etc. It is suitable for different underwater environments and sampling requirements. It can also be functionally expanded and upgraded according to actual needs to improve the flexibility and adaptability of the system.
[0056] This underwater water sample collection system composed of a pressure sensor, a microcontroller, and an actuator can significantly improve the sampling accuracy and asepticity, enhance the control ability of the sampling depth, improve the sampling efficiency and data reliability, reduce costs, and enhance the system adaptability; it has broad application prospects in the fields of water quality monitoring, scientific research, etc.
[0057] In a specific feasible implementation, the pressure sensor, the microcontroller, and the actuator are integrally integrated.
[0058] In a specific feasible implementation, it further includes a housing, and the pressure sensor, the microcontroller, and the actuator are arranged inside the housing, where
[0059] a double-layer sealed hatch is provided on the housing.
[0060] In a specific feasible implementation, the double-layer sealed hatch includes a sterile film 3 on the inner layer and a pressure-resistant hatch cover 4 on the outer layer.
[0061] In a specific feasible implementation, the housing includes a frame 5 and a folding layer 6, where
[0062] the frame is arranged inside the folding layer,
[0063] the frame is connected to the folding layer and is used to fold the folding layer.
[0064] In a specific feasible implementation, the frame includes a lightweight titanium alloy frame.
[0065] In a specific feasible implementation, the folding layer includes a flexible silicone folding layer.
[0066] In a specific feasible implementation, a self-cleaning system is arranged inside the housing for sterilization.
[0067] In a specific feasible implementation, a hydrophobic coating is provided on the surface of the housing to reduce the turbulent resistance.
[0068] Specifically, referring to Figures 1 to 3 , the bacterial water sample collection device adopts a modular split design and includes:
[0069] Intelligent trigger module: Integrating a pressure sensor, a microcontroller, and a mechanical actuator, it judges the underwater depth in real time through the pressure value and triggers sampling after reaching the preset depth.
[0070] Sterile sampling unit: Comprising a double-layer sealed hatch (the inner layer is a sterile film and the outer layer is a pressure-resistant hatch cover), the hatch is closed before sampling, and the hatch automatically opens and inhales a quantitative water sample after being triggered.
[0071] Folding portable structure: The device housing adopts a lightweight titanium alloy frame and a flexible silicone folding layer. When not in use, it is compressed to the size of a palm (about 15 cm × 10 cm), and forms a stable sampling structure after unfolding. A telescopic rod 11 is arranged between the two parts of the frame. When in use, manually pull the telescopic rod to unfold the housing. Specifically, a push rod motor can also be set to unfold the housing to achieve electric control.
[0072] Self-cleaning system: Sterilize the sampling chamber through the built-in ultraviolet lamp 7 before and after sampling to avoid cross-contamination.
[0073] In this embodiment, a trigger mechanism combining depth control and sterility is adopted; the pressure sensor monitors the water depth in real time. When the preset depth is reached, the signal is transmitted to the micro motor to drive the double-layer hatch to open synchronously (depth control process). The inner sterile membrane isolates the external water body through a biocompatible material (such as polytetrafluoroethylene) to avoid pollution;
[0074] After sampling is completed, when the hatch closes, it triggers the external airbag 8 to open, and the sampling chamber can float to the water surface through the buoyancy of the airbag; the airbag and the counterweight cooperate to achieve suspension in water and control the depth.
[0075] Quantitative sampling method: Adopt a piston-type negative pressure inhalation design, and control the inhalation volume through a precision cylinder 9 (error rate < 1%), and different standard volumes of 50 mL - 500 mL can be preset.
[0076] Anti-environmental interference design: The surface of the housing is covered with a hydrophobic coating to reduce the turbulence resistance; the core circuit is encapsulated in a silicone waterproof cabin, and the pressure resistance depth reaches 50 meters.
[0077] The working process of the bacterial water sample collection device is as follows:
[0078] 1: Device preparation
[0079] Unfold the device in the folded state, connect to a portable handheld terminal (mobile phone or handheld controller), and set the target depth (0.1 - 10 m) and sampling volume (such as 100 mL). Add a sterile membrane to the pressure-resistant hatch cover (the sterile membrane can be sterilized in the laboratory by yourself and taken to the field for use, or you can buy a commercially available sterilized sterile membrane); the handheld terminal is radio-connected to the microcontroller.
[0080] Start the ultraviolet self-cleaning system installed inside the sampling chamber on the portable terminal to sterilize the sampling chamber and the sterile membrane again for 30 seconds (sterilization process).
[0081] Step 2: Deployment and sinking
[0082] Put the device into the water, the folding structure automatically unfolds into a streamlined shape, and the built-in counterweight 12 makes it sink evenly.
[0083] Step 3: Deep trigger sampling
[0084] When the pressure sensor detects that the water depth reaches the designed depth, the controller sends a signal, and the micro-motor 10 drives the outer pressure-resistant hatch to open. At the same time, the inner sterile film ruptures, and the negative pressure piston sucks in 100 mL of water sample (sampling process).
[0085] Step 4: Sealing and recovery
[0086] When the sampling volume reaches the set value, the hatch closes, and the device automatically floats to the water surface through the airbag.
[0087] In the above technical solution, the beneficial effects include:
[0088] Dual insurance design of depth and sterility: For the first time, pressure sensing trigger is combined with double-layer sterile hatch, solving the contradiction of traditional devices that are "accurate in depth but easy to be contaminated" or "good in sterility but poor in depth".
[0089] Folding lightweight structure: The volume is reduced by 60% compared with similar products, and the weight is controlled within 0.8 kg, which can be carried in a backpack.
[0090] High-precision quantitative sampling: The piston-type negative pressure design ensures that the sampling volume error rate is <1%, which is better than the international standard (5%).
[0091] Strong environmental adaptability: It can withstand a pressure of 50 meters, and the working temperature is -20°C to 50°C;
[0092] The hydrophobic coating reduces the turbulence interference, and the sampling success rate is increased by 40%.
[0093] Long-term sterile guarantee: Double sterilization by ultraviolet light + biofilm, and the colony contamination rate is lower than 0.1%.
[0094] It is applicable to the field water sample collection of disease control centers, environmental protection departments, and scientific research institutions, especially suitable for operations in remote areas or extreme environments.
[0095] In Figure 4 this application embodiment provides a method for collecting bacterial water samples, including the following steps:
[0096] Set the sampling parameters by using a handheld terminal; the sampling parameters include water depth and sampling volume data;
[0097] Collect the water pressure by using a pressure sensor and send the collected pressure value to the microcontroller;
[0098] Use the microcontroller to judge the underwater depth according to the received pressure value, and send a sampling instruction to the actuator according to the depth value;
[0099] Collect the water sample by using the actuator.
[0100] In the above technical solution, a pressure sensor is provided to collect the water pressure and send the collected pressure value to the microcontroller; the microcontroller is used to judge the underwater depth according to the received pressure value and send a sampling instruction to the actuator according to the depth value; the actuator is used to collect water samples; the aseptic sampling depth and sampling accuracy of the collection device are improved.
[0101] Those skilled in the art of the present application know that the present application can be implemented as a system, a method, or a computer program product.
[0102] Therefore, the present disclosure can be specifically implemented in the following forms, that is: it can be completely hardware, can also be completely software (including firmware, resident software, microcode, etc.), and can also be a combination of hardware and software, which is generally referred to as "circuit", "module" or "system" in this article. In addition, in some embodiments, the present application can also be implemented in the form of a computer program product in one or more computer-readable media, and the computer-readable media contains computer-readable program code.
[0103] Any combination of one or more computer-readable media can be adopted. The computer-readable media can be a computer-readable signal medium or a computer-readable storage medium. The computer-readable storage medium can be, for example, but not limited to, an electrical, magnetic, optical, electromagnetic, infrared, or semiconductor system, device, or component, or any combination of the above. More specific examples (non-exhaustive list) of the computer-readable storage medium include: an electrical connection with one or more wires, a portable computer disk, a hard disk, a random access memory (RAM), a read-only memory (ROM), an erasable programmable read-only memory (EPROM or flash memory), an optical fiber, a portable compact disk read-only memory (CD-ROM), an optical storage device, a magnetic storage device, or any suitable combination of the above. In this document, the computer-readable storage medium can be any tangible medium that contains or stores a program, and the program can be used by or in combination with an instruction execution system, device, or component.
[0104] Although the embodiments of the present application have been shown and described above, it can be understood that the above embodiments are exemplary and should not be construed as limitations on the present application. Those of ordinary skill in the art can make changes, modifications, substitutions, and variations to the above embodiments within the scope of the present application. On this basis, various substitutions and improvements can be made to the present application, and these all fall within the protection scope of the present application.
Claims
1. A bacterial water sample collection device, characterized in that: include: A pressure sensor, used to collect water pressure and send the collected pressure value to the microcontroller; The microcontroller is used to determine the underwater depth according to the received pressure value, and send a sampling instruction to the actuator according to the depth value; The actuator is used for collecting water samples.
2. The bacteria water sample collection device according to claim 1, characterized in that: The pressure sensor, the microcontroller and the actuator are integrated into one piece.
3. The bacteria water sample collection device according to claim 2, characterized in that: It also includes a housing, in which the pressure sensor, the microcontroller and the actuator are arranged, wherein: A double-layer sealed door is arranged on the shell.
4. The bacteria water sample collection device according to claim 3, characterized in that: The double-layer sealed hatch door comprises an inner sterile membrane and an outer pressure-resistant hatch cover.
5. The bacteria water sample collection device according to claim 4, characterized in that: The shell comprises a frame and a folding layer, wherein: The frame is arranged in the folding layer, The frame is connected to the folding layer and is used for folding the folding layer.
6. The bacteria water sample collection device according to claim 5, characterized in that: The frame comprises a lightweight titanium alloy frame.
7. The bacteria water sample collection device according to claim 6, characterized in that: The folding layer comprises a flexible silicone folding layer.
8. The bacteria water sample collection device according to claim 7, characterized in that: A self-cleaning system is arranged in the shell for sterilization.
9. The bacteria water sample collection device according to claim 8, characterized in that: The surface of the shell is provided with a hydrophobic coating to reduce turbulent resistance.
10. A method for collecting bacterial water samples, characterized in that: The following steps are involved: Using a pressure sensor to collect water pressure and send the collected pressure value to a microcontroller; Using the microcontroller to determine the underwater depth according to the received pressure value, and sending a sampling instruction to the actuator according to the depth value; The actuator is used to collect water samples.