Medical equipment

Abstract

The invention discloses a medical device. The medical equipment comprises an electric control box, a plate washing machine, a liquid path system, an oscillation incubation machine and a magnetic eraser, the plate washing machine comprises a magnetic plate base, an oscillation assembly and a liquid injection assembly, the liquid path system is communicated with the liquid injection assembly, the plate washing machine and the liquid path system are matched to purify magnetic beads, and the oscillation incubation machine comprises a reagent plate, an oscillation driving assembly and a heating assembly. The oscillation driving assembly can drive the reagent plate to oscillate in a horizontal plane, the heating assembly is used for heating the reagent plate, so that constant-temperature incubation of a reaction reagent can be realized, and the magnetic eraser is used for demagnetizing magnetic beads. According to the medical equipment, the plate washing machine, the oscillation incubation machine and the magnetic eraser are at least partially integrated on the electric control box, and the plate washing machine, the oscillation incubation machine and the magnetic eraser share one set of power supply assembly and one set of communication assembly, so that the integration level of the medical equipment is improved, the size is reduced, the cost is reduced, the operation of the medical equipment is simplified, and the working efficiency of the medical equipment is improved. And the workload of the user is reduced.

Description

Technical Field

[0001] The present invention relates to the technical field of in vitro diagnosis, and in particular to a medical device. Background Art

[0002] In the field of in vitro diagnosis, especially in immunoassays, nucleic acid research, enzymatic reactions, receptor ligand recognition and other research fields, plate washers for magnetic bead purification and devices for constant temperature incubation, oscillation and demagnetization of reaction reagents are needed. In the prior art, plate washers, demagnetization devices and constant temperature incubation devices are mostly independent stand-alone machines, and each independent instrument requires a power supply and a computer control, which not only leads to low integration of the entire equipment and a large footprint, but also makes the equipment operation cumbersome, and users need to switch between multiple instruments constantly. In addition, the cost of multiple stand-alone machines is significantly higher than that of an integrated whole machine. Summary of the invention

[0003] The object of the present invention is to provide a medical device with high integration, small footprint, simple operation and low manufacturing cost.

[0004] To achieve this object, the present invention adopts the following technical solutions:

[0005] A medical device, comprising: an electric control box, in which a power supply component and a communication component are arranged; a plate washer, which comprises a magnetic plate seat, an oscillating component and a liquid injection component, wherein the magnetic plate seat is used to install a liquid injection plate, the oscillating component is used to drive the magnetic plate seat to oscillate in a horizontal direction, and the liquid injection component is used to inject liquid into the liquid injection plate or to absorb liquid from the liquid injection plate; a liquid circuit system, which is connected to the liquid injection component and is used to inject liquid into the liquid injection component and to absorb liquid from the liquid injection component; an oscillating incubator, wherein The oscillating incubator includes a reagent plate, an oscillating drive component and a heating component, wherein the oscillating drive component is transmission-connected to the reagent plate and is used to drive the reagent plate to oscillate in a horizontal plane, and the heating component is used to heat the reagent plate; a demagnetizer, which is used to demagnetize the magnetic beads in the injection plate placed thereon; wherein the plate washer, the oscillating incubator and the demagnetizer are at least partially integrated on the electrical control box, and the plate washer, the oscillating incubator and the demagnetizer share a set of the power supply components and a set of the communication components.

[0006] Preferably, the magnetic plate seat comprises a mounting seat, a magnetic plate and an elastic clamping piece, the mounting seat is provided with a mounting cavity, the magnetic plate is arranged in the mounting cavity, and the elastic clamping piece is used for clamping the liquid injection plate placed on the magnetic plate.

[0007] Preferably, the oscillation assembly includes a first oscillation motor, a first screw rod, a first nut block and a first connecting block, the motor shaft of the first oscillation motor is rotatably connected to one end of the first screw rod and can drive the first screw rod to rotate, the first nut block is threadedly connected to the first screw rod, and the first connecting block is connected between the first nut block and the magnetic plate seat.

[0008] Preferably, the injection assembly includes an injection mechanism and an injection drive mechanism, the injection mechanism is movably arranged above the magnetic plate seat, the injection drive mechanism is transmission-connected to the injection mechanism and is used to drive the injection mechanism to approach or move away from the injection plate.

[0009] Preferably, the injection assembly includes an injection mechanism, which is movably arranged above the magnetic plate seat and is used to inject liquid into the injection plate or absorb liquid from the injection plate; the injection mechanism includes an injection block, an injection needle and a drainage needle, and the injection block is provided with an injection groove and a drainage groove, the injection groove is respectively connected to the injection needle and the injection pipeline of the liquid circuit system, the drainage groove is respectively connected to the drainage needle and the drainage pipeline of the liquid circuit system, and the injection port of the injection needle is higher than the drainage port of the drainage needle.

[0010] Preferably, the injection drive mechanism includes a first injection motor, an injection timing belt, a connecting structure, a second injection motor, a second screw rod and a second nut block. The motor shaft of the first injection motor is connected to the connecting structure through the injection timing belt, and can drive the connecting structure to move in a horizontal direction. The second injection motor is arranged on the connecting structure. The motor shaft of the second injection motor is transmission-connected to one end of the second screw rod and can drive the second screw rod to rotate around a vertical axis. The second nut block is threadedly connected to the second screw rod, and the second nut block is connected to the injection mechanism.

[0011] Preferably, the injection assembly includes an injection mechanism, which is movably arranged above the magnetic plate seat and is used to inject liquid into the injection plate or absorb liquid from the injection plate; the liquid circuit system includes a cleaning liquid bottle, a distilled water bottle, a waste liquid bottle, a plunger pump, a two-position three-way solenoid valve and a two-position two-way solenoid valve, the cleaning liquid bottle is connected to the first liquid inlet of the two-position three-way solenoid valve, the distilled water bottle is connected to the second liquid inlet of the two-position three-way solenoid valve, the liquid outlet port of the two-position three-way solenoid valve is connected to the plunger pump through a first branch pipe, the liquid inlet port of the two-position two-way solenoid valve is connected to the plunger pump through a second branch pipe, the liquid outlet port of the two-position two-way solenoid valve is connected to the liquid inlet of the injection mechanism, and the liquid outlet of the injection mechanism is connected to the waste liquid bottle through a waste liquid pipe.

[0012] Preferably, the liquid injection mechanism includes two liquid outlets arranged at intervals. The waste liquid pipe is a tee pipe. Two branches of the waste liquid pipe are respectively communicated with the two liquid outlets, and the main pipe of the waste liquid pipe is communicated with the waste liquid bottle.

[0013] Preferably, the oscillation driving assembly includes a second oscillation motor, an eccentric structure and an oscillation bottom plate. The eccentric structure includes an eccentric bottom plate and an eccentric shaft. The eccentric shaft is eccentrically arranged on the eccentric bottom plate. The motor shaft of the second oscillation motor is connected to the eccentric bottom plate. A bearing is arranged on the oscillation bottom plate, and the eccentric shaft is rotatably connected in the bearing. The oscillation bottom plate is connected to the reagent plate.

[0014] Preferably, the oscillation incubator further includes an installation table. The oscillation bottom plate is arranged on the tabletop of the installation table. A guiding mechanism is arranged on the installation table. A guiding and limiting hole is arranged on the oscillation bottom plate. The guiding mechanism includes a positioning pin protruding from the tabletop, an elastic ring and a roller sleeved on the positioning pin. The radial dimension of the elastic ring is larger than the radial dimension of the roller, and the elastic ring and the roller are movably arranged in the guiding and limiting hole.

[0015] Preferably, the heating assembly includes a heating bottom plate, a heating film, a temperature sensor and a temperature control protection switch. The heating bottom plate is laminated under the reagent plate. The heating film is arranged on the heating bottom plate. The temperature sensor is used for detecting the heating temperature of the heating film, and the temperature control protection switch is used for cutting off the power supply to protect the heating film.

[0016] Preferably, the plate washer, the oscillation incubator and the demagnetizer are all arranged on the electric control box. The oscillation incubator and the demagnetizer are arranged at intervals in the first direction, and the plate washer is arranged side by side with the oscillation incubator and the demagnetizer in the second direction.

[0017] Advantages of the present invention:

[0018] The medical equipment provided by the present invention includes an electric control box, a plate washer, a liquid circuit system, an oscillating incubator, and a demagnetizer. A power supply component and a communication component are arranged in the electric control box. The plate washer includes a magnetic plate seat, an oscillating component and a liquid injection component. The liquid circuit system is connected to the liquid injection component. The plate washer and the liquid circuit system cooperate to realize the purification of magnetic beads. The oscillating incubator includes a reagent plate, an oscillating drive component and a heating component. The oscillating drive component is connected to the reagent plate in a transmission manner and is used to drive the reagent plate to oscillate in a horizontal plane. The heating component is used to heat the reagent plate, so as to realize the constant temperature incubation of the reaction reagent. The demagnetizer is used to demagnetize the magnetic beads. The medical equipment is at least partially integrated with the plate washer, the oscillating incubator and the demagnetizer on the electric control box, and the plate washer, the oscillating incubator and the demagnetizer share a set of power supply components and a set of communication components. It not only improves the integration of the medical equipment, reduces the volume, reduces the cost, but also simplifies the operation of the medical equipment and reduces the workload of the user. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] Figure 1 is a schematic diagram of a medical device provided by an embodiment of the present invention;

[0020] Figure 2 is a schematic diagram of a plate washer provided in an embodiment of the present invention;

[0021] Figure 3 is a schematic diagram of a partial structure of a plate washer provided by an embodiment of the present invention at a certain viewing angle;

[0022] Figure 4 yes Figure 3 A schematic diagram of the structure shown in another viewing angle;

[0023] Figure 5 yes Figure 3 A schematic diagram of the structure shown at another viewing angle;

[0024] Figure 6 is a schematic diagram of a liquid injection mechanism provided in an embodiment of the present invention;

[0025] Figure 7 is a side view of a liquid injection mechanism provided by an embodiment of the present invention;

[0026] Figure 8 is a schematic diagram of a fluid circuit system provided by an embodiment of the present invention;

[0027] Figure 9 is a schematic diagram of an oscillating incubator provided in an embodiment of the present invention;

[0028] Figure 10 is an exploded view of the oscillating incubator provided by an embodiment of the present invention at a certain viewing angle;

[0029] Figure 11It is an exploded view of the oscillating incubator provided by an embodiment of the present invention from another perspective;

[0030] Figure 12 It is a schematic diagram of the guiding mechanism provided by an embodiment of the present invention;

[0031] Figure 13 It is a connection schematic diagram of the power supply component provided by an embodiment of the present invention;

[0032] Figure 14 It is a connection schematic diagram of the communication component provided by an embodiment of the present invention.

[0033] In the figure:

[0034] 100, electric control box;

[0035] 200, plate washer; 210, magnetic plate seat; 211, mounting seat; 212, magnetic plate; 213, elastic clamping member; 220, oscillation assembly; 221, first oscillation motor; 222, first lead screw; 223, first nut block; 224, first connecting block; 225, first guide rail; 230, liquid injection mechanism; 231, liquid injection block; 2311, liquid injection block body; 2312, liquid injection block cover plate; 2313, liquid injection groove; 2314, drain groove; 232, liquid injection needle; 233, drain needle; 234, sealing gasket; 240, liquid injection driving mechanism; 241, first liquid injection motor; 242, liquid injection synchronous belt; 243, second connecting block; 244, third connecting block; 245, second liquid injection motor; 246, fourth connecting block; 247, second guide rail; 248, third guide rail; 250, support seat; 260, waste liquid tank;

[0036] 300, liquid path system; 301, cleaning liquid bottle; 302, distilled water bottle; 303, waste liquid bottle; 304, plunger pump; 305, two-position three-way solenoid valve; 306, two-position two-way solenoid valve; 307, first branch pipe; 308, second branch pipe; 309, first check valve; 310, second check valve; 311, waste liquid pipe; 312, diaphragm pump; 313, waste liquid collection bottle;

[0037] 400, oscillating incubator; 410, reagent plate; 411, positioning block; 420, oscillating drive assembly; 421, second oscillating motor; 422, eccentric structure; 4221, eccentric bottom plate; 4222, eccentric shaft; 423, oscillating bottom plate; 4231, bearing; 4232, guide limit hole; 4233, support hole; 430, heating assembly; 431, heating bottom plate; 432, heating film; 433, temperature sensor; 434, temperature control protection switch; 440, mounting table; 450, guide mechanism; 451, positioning pin; 452, elastic ring; 453, roller; 460, support rod; 470, adapter circuit board; 480, motor control board; 491, optical coupler; 492, optical coupler baffle;

[0038] 500. Demagnetizer. DETAILED DESCRIPTION

[0039] The technical solution of the present invention will be described clearly and completely below in conjunction with the accompanying drawings. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0040] In the description of the present invention, it should be noted that the terms "center", "up", "down", "left", "right", "vertical", "horizontal", "inside", "outside" and the like indicate positions or positional relationships based on the positions or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as limiting the present invention. In addition, the terms "first" and "second" are used for descriptive purposes only, and cannot be understood as indicating or implying relative importance. Among them, the terms "first position" and "second position" are two different positions.

[0041] In the description of the present invention, it should be noted that, unless otherwise clearly specified and limited, the terms "installed", "connected", and "connected" should be understood in a broad sense, for example, it can be a fixed connection or a detachable connection; it can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium, and it can be the internal communication of two components. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0042] The present invention provides a medical device, which is used in the field of in vitro diagnosis and can realize constant temperature incubation and oscillation of reaction reagents, as well as purification and demagnetization of magnetic beads. Figures 1 - 14 The specific structure of the medical device according to the embodiment of the present invention is described.

[0043] As shown Figures 1 to 14 in the figure, the medical device includes an electric control box 100, a plate washer 200, a liquid path system 300, an oscillation incubator 400, and a demagnetizer 500. A power supply component and a communication component are provided in the electric control box 100. The power supply component is used to provide electric energy, and the communication component is used to realize the communication connection between various components. The electric control box 100 includes a box body, and the box body forms a receiving cavity. The power supply component and the communication component are both arranged in the receiving cavity.

[0044] The plate washer 200 is used to purify magnetic beads. The plate washer 200 includes a magnetic plate seat 210, an oscillation component 220, and a liquid injection component. The magnetic plate seat 210 is used to install a liquid injection plate. In some embodiments, the liquid injection plate is a microplate, such as a 96-well microplate. The oscillation component 220 is used to drive the magnetic plate seat 210 to oscillate in the horizontal direction to achieve sufficient mixing of the liquid and magnetic beads in the microplate. Here, the horizontal direction can be the front-back direction, left-right direction of the medical device, or any horizontal direction. The liquid injection component is used to inject liquid into the liquid injection plate or suck liquid from the liquid injection plate. Optionally, the liquid injection component includes a liquid injection mechanism 230 and a liquid injection driving mechanism 240. The liquid injection mechanism 230 is movably arranged above the magnetic plate seat 210. The liquid injection driving mechanism 240 is in transmission connection with the liquid injection mechanism 230 and is used to drive the liquid injection mechanism 230 to approach or move away from the liquid injection plate. The liquid path system 300 is communicated with the liquid injection component, specifically with the liquid injection mechanism 230. The liquid path system 300 and the liquid injection component cooperate to be able to inject liquid into the liquid injection plate and suck liquid from the liquid injection plate.

[0045] The oscillation incubator 400 can realize the constant-temperature incubation and oscillation of reaction reagents. The oscillation incubator 400 includes a reagent plate 410, an oscillation driving component 420, and a heating component 430. The oscillation driving component 420 is in transmission connection with the reagent plate 410 and is used to drive the reagent plate 410 to oscillate in the horizontal plane. The heating component 430 is used to heat the reagent plate 410 so that the reaction reagents in the reagent plate 410 are in a constant-temperature incubation environment.

[0046] The demagnetizer 500 is used to demagnetize the magnetic beads in the liquid injection plate placed thereon, so that the magnetic beads are separated from the samples adsorbed on the magnetic beads. Moreover, the plate washer 200, the oscillation incubator 400, and the demagnetizer 500 are at least partially integrated on the electric control box 100, and the plate washer 200, the oscillation incubator 400, and the demagnetizer 500 share a set of power supply components and a set of communication components.

[0047] Compared with the devices that implement different functions in the prior art, which are independently set and each has an independent power component and communication component, the medical device provided by the present invention integrates at least part of the plate washer 200, the oscillating incubator 400, and the demagnetizer 500 on the electronic control box 100, and the plate washer 200, the oscillating incubator 400, and the demagnetizer 500 share a set of power components and a set of communication components. Thereby, not only the integration degree of the medical device is improved, the volume is reduced, and the cost is lowered, but also the operation of the medical device can be simplified, and the user can easily grasp the operations and data of each process of the experiment, thus greatly reducing the workload of the user.

[0048] In some embodiments, as Figure 1 shown, the plate washer 200, the oscillating incubator 400, and the demagnetizer 500 are all arranged on the electronic control box 100, and the oscillating incubator 400 and the demagnetizer 500 are spaced apart in the first direction, and the plate washer 200 is arranged side by side with the oscillating incubator 400 and the demagnetizer 500 in the second direction. Such a layout makes the integration degree of the medical device higher and makes it easier for the user to operate.

[0049] As Figures 2 to 5 shown, the plate washer 200 includes a support base 250, and a magnetic plate base 210 is installed on the top surface of the support base 250. The magnetic plate base 210 includes a mounting base 211, a magnetic plate 212, and an elastic clamping member 213. The mounting base 211 is provided with a mounting cavity, the magnetic plate 212 is arranged in the mounting cavity, and the elastic clamping member 213 is used to clamp the liquid injection plate placed on the magnetic plate 212. Optionally, the elastic clamping member 213 is a spring piece, and the spring piece can clamp the liquid injection plate tightly to prevent noise caused by gaps during the oscillation process.

[0050] In some embodiments, the magnetic plate base 210 is generally in a cubic shape, the magnetic plate 212 is a rectangular plate, and a plurality of cylindrical strong magnets arranged in a horizontal row are installed in the magnetic plate 212. Optionally, the top surface of the cylindrical strong magnet is slightly lower than the top plate of the magnetic plate 212, and the number of the cylindrical strong magnets is 96, so that the 96-well microplate can be inserted into the 96 cylindrical strong magnets one by one. After the microplate is inserted into the magnetic plate 212, the magnetic beads in the microplate are attached to the bottom of the magnetic plate 212 under the magnetic field of the magnetic plate 212, thereby facilitating the oscillating cleaning of the reaction solution in the microplate.

[0051] Continue to refer to Figure 3As shown, the oscillation assembly 220 is installed in the support base 250. In some embodiments, the oscillation assembly 220 includes a first oscillation motor 221, a first lead screw 222, a first nut block 223, and a first connection block 224. The first oscillation motor 221 is installed on the support base 250. The motor shaft of the first oscillation motor 221 is rotatably connected to one end of the first lead screw 222 and can drive the first lead screw 222 to rotate. The first nut block 223 is threadedly connected to the first lead screw 222. The first connection block 224 is connected between the first nut block 223 and the magnetic plate base 210.

[0052] During the actual working process, driven by the first oscillation motor 221, the first lead screw 222 can rotate around its central axis, and the first nut block 223 can move along the central axis of the first lead screw 222. The first connection block 224 connected to the first nut block 223 moves synchronously, thereby driving the magnetic plate base 210 to move. As the first oscillation motor 221 switches the rotation direction of the motor shaft, the magnetic plate base 210 can oscillate repeatedly in the oscillation direction, so as to realize sufficient oscillating cleaning of the reaction solution in the microplate.

[0053] Optionally, the oscillation assembly 220 further includes a first guide rail 225. The first guide rail 225 extends along the oscillation direction. The first connection block 224 is slidably connected to the first guide rail 225. The setting of the first guide rail 225 can improve the moving accuracy of the magnetic plate base 210 in the oscillation direction.

[0054] Continue to refer to Figure 6 and Figure 7 As shown, in some embodiments, the liquid injection mechanism 230 includes a liquid injection block 231, a liquid injection needle 232, and a liquid discharge needle 233. A liquid injection groove 2313 and a liquid discharge groove 2314 are provided in the liquid injection block 231. The liquid injection groove 2313 is respectively communicated with the liquid injection needle 232 and the liquid injection pipeline of the liquid path system 300. The liquid discharge groove 2314 is respectively communicated with the liquid discharge needle 233 and the liquid discharge pipeline of the liquid path system 300. And the liquid injection port of the liquid injection needle 232 is higher than the liquid discharge port of the liquid discharge needle 233.

[0055] Optionally, the liquid injection block 231 includes a liquid injection block main body 2311 and a liquid injection block cover plate 2312. A liquid injection groove 2313 and a liquid discharge groove 2314 are formed in the liquid injection block main body 2311, and the liquid injection groove 2313 and the liquid discharge groove 2314 penetrate through the liquid injection block main body 2311 in the vertical direction. Optionally, the liquid injection groove 2313 and the liquid discharge groove 2314 are kidney-shaped grooves arranged side by side. A liquid injection port communicating with the liquid injection groove 2313 and a liquid discharge port communicating with the liquid discharge groove 2314 are formed on one side of the liquid injection block main body 2311. Optionally, a sealing groove is further provided at a position on the top surface of the liquid injection block main body 2311 directly opposite to the tops of the liquid injection groove 2313 and the liquid discharge groove 2314. The sealing gasket 234 is placed in the sealing groove, which can improve the sealing performance between the liquid injection block main body 2311 and the liquid injection block cover plate 2312.

[0056] The liquid injection needle 232 and the liquid discharge needle 233 are connected below the liquid injection block main body 2311, and can be set to multiple according to requirements. Multiple liquid injection needles 232 are arranged in a row or a column and communicate with the liquid injection groove 2313 at the same time; multiple liquid discharge needles 233 are arranged in a row or a column and communicate with the liquid discharge groove 2314 at the same time. Optionally, the microplate includes micro-holes arranged in n rows and m columns. There are n liquid injection needles 232 and n liquid discharge needles 233, which are arranged side by side in a column; or, there are m liquid injection needles 232 and m liquid discharge needles 233, which are arranged side by side in a row. In a specific embodiment, both the liquid injection needle 232 and the liquid discharge needle 233 are provided with 8.

[0057] Continue to refer to Figures 3 to 5 As shown, in some embodiments, the liquid injection driving mechanism 240 includes a first liquid injection motor 241, a liquid injection synchronous belt 242, and a connection structure. The liquid injection synchronous belt 242 includes a first pulley, a second pulley, and a synchronous belt body. The synchronous belt body is sleeved on the first pulley and the second pulley. The first liquid injection motor 241 is connected to the first pulley and is used to drive the first pulley to rotate. The connection structure is connected to the synchronous belt body and is connected to the liquid injection mechanism 230. Driven by the first liquid injection motor 241, the liquid injection mechanism 230 can move in the horizontal direction, so as to realize the alignment of the liquid injection needle 232 and the liquid discharge needle 233 with the micro-holes in different rows or different columns.

[0058] In some embodiments, the liquid injection driving mechanism 240 includes a second liquid injection motor 245, a second lead screw, and a second nut block. The motor shaft of the second liquid injection motor 245 is in transmission connection with one end of the second lead screw and can drive the second lead screw to rotate around the vertical axis. The second nut block is threadedly connected to the second lead screw, and the second nut block is connected to the liquid injection mechanism 230. Driven by the second liquid injection motor 245, the liquid injection mechanism 230 can move up and down in the vertical direction, so that the liquid injection needle 232 and the liquid discharge needle 233 can descend into the micro-holes for liquid injection or liquid suction, or the liquid injection needle 232 and the liquid discharge needle 233 can rise to the avoidance position.

[0059] Optionally, the second liquid injection motor 245 is arranged on the connection structure, so that the liquid injection mechanism 230 can move in the horizontal direction and also in the vertical direction. Further optionally, the connection structure includes a second connection block 243 and a third connection block 244 which are connected to each other. The second connection block 243 is connected to the synchronous belt body. The liquid injection driving mechanism 240 further includes a second guide rail 247. The third connection block 244 is slidably connected to the second guide rail 247. The arrangement of the second guide rail 247 can improve the moving accuracy of the liquid injection mechanism 230 in the horizontal direction.

[0060] Furthermore, the liquid injection driving mechanism 240 includes a fourth connection block 246 and a third guide rail 248. The third guide rail 248 is arranged in the vertical direction. The fourth connection block 246 is connected to the third guide rail 248, and the fourth connection block 246 is connected to the second nut block. The arrangement of the third guide rail 248 can improve the moving accuracy of the liquid injection mechanism 230 in the vertical direction.

[0061] Continue to refer to Figure 5 As shown, a waste liquid tank 260 is further arranged on one side of the magnetic plate seat 210. The waste liquid tank 260 is used for holding waste liquid.

[0062] Continue to refer to Figure 8 As shown, in some embodiments, the liquid path system 300 includes a cleaning liquid bottle 301, a distilled water bottle 302, a waste liquid bottle 303, a plunger pump 304, a two-position three-way solenoid valve 305, and a two-position two-way solenoid valve 306. The cleaning liquid bottle 301 contains a reaction solution for cleaning magnetic beads. The distilled water bottle 302 contains distilled water for easily cleaning the entire pipeline, thereby avoiding the pipeline or the pinholes being blocked by solution crystallization. The cleaning liquid bottle 301 is communicated with the first liquid inlet of the two-position three-way solenoid valve 305. The distilled water bottle 302 is communicated with the second liquid inlet of the two-position three-way solenoid valve 305. The liquid outlet port of the two-position three-way solenoid valve 305 is communicated with the plunger pump 304 through a first branch pipe 307. The liquid inlet port of the two-position two-way solenoid valve 306 is communicated with the plunger pump 304 through a second branch pipe 308. The liquid outlet port of the two-position two-way solenoid valve 306 is communicated with the liquid inlet of the liquid injection mechanism 230. The liquid outlet of the liquid injection mechanism 230 is communicated with the waste liquid bottle 303 through a waste liquid pipe 311. Optionally, the first branch pipe 307 and the second branch pipe 308 can be replaced by a three-way pipeline.

[0063] In some embodiments, the liquid injection mechanism 230 includes two liquid outlets arranged at intervals. The waste liquid pipe 311 is a three-way pipeline. The three-way pipeline includes two branch pipes, namely a third branch pipe and a fourth branch pipe. The two branch pipes are respectively communicated with the two liquid outlets. The main pipe of the waste liquid pipe 311 is communicated with the waste liquid bottle 303.

[0064] In some embodiments, a first one-way valve 309 is provided on the first branch pipe 307, and a second one-way valve 310 is provided on the second branch pipe 308.

[0065] In some embodiments, the waste liquid bottle 303 is a vacuum bottle, and the liquid path system 300 further includes a vacuum pump. The top of the waste liquid bottle 303 is provided with two openings, a first opening and a second opening. The main pipe of the waste liquid pipe 311 is communicated with the first opening of the waste liquid bottle 303, and the suction port of the vacuum pump is communicated with the second opening of the waste liquid bottle 303 through a pipeline. In some parallel embodiments, the liquid path system 300 further includes a diaphragm pump 312 and a waste liquid collection bottle 313. The waste liquid collection bottle 313 has two openings, a third opening and a fourth opening. The second opening of the waste liquid bottle 303 is communicated with the inlet of the diaphragm pump 312, the outlet of the diaphragm pump 312 is communicated with the third opening of the waste liquid collection bottle 313, and the fourth opening of the waste liquid collection bottle 313 is communicated with the waste liquid tank 260 through a pipeline, so as to realize the sharing of the waste liquid tank 260 and the waste liquid bottle 303 for the waste liquid collection bottle 313.

[0066] The plunger pump 304 is used to provide power for liquid injection and liquid suction. The plunger pump 304 performs the functions of liquid suction and liquid discharge through the up and down movement of the piston. The two-way three-way solenoid valve 305 controls the plunger pump 304 to suck the cleaning liquid from the cleaning liquid bottle 301 or suck the distilled water from the distilled water bottle 302. In one embodiment, when the two-way three-way solenoid valve 305 is opened, distilled water is sucked, and when the two-way three-way solenoid valve 305 is closed, the cleaning liquid is sucked. The first one-way valve 309 is connected to the two-way three-way solenoid valve 305, which can not only ensure that the liquid discharged by the plunger pump 304 will not flow back to the cleaning liquid bottle 301 or the distilled water bottle 302, but also the second one-way valve 310 ensures that the liquid in the pipeline connected to the two-way two-way solenoid valve 306 will not be sucked during liquid suction. When the plunger pump 304 discharges liquid, the liquid passes through the main pipe of the three-way pipeline and the second branch pipe 308, and enters the liquid injection groove 2313 of the liquid injection block 231 through the two-way two-way solenoid valve 306, and is injected into the 96-well microplate through the liquid injection needle 232. The microplate is arranged in an 8×12 arrangement, and 8 holes can be injected at a time. The third branch pipe and the fourth branch pipe of the other three-way pipeline are respectively communicated with the two liquid outlets of the liquid injection block 231, which can evenly discharge the pressure values of each needle hole in the liquid discharge groove 2314. The main pipe of this three-way pipeline is connected to the first opening of the waste liquid bottle 303, the second opening of the waste liquid bottle 303 is connected to the suction port of the vacuum pump, and the air outlet of the vacuum pump is connected to the atmosphere. When the vacuum pump works, the air in the waste liquid bottle 303 is pumped out to form a negative pressure environment, so that a suction force is generated at the needle tip of the liquid discharge needle 233, and then the reaction liquid in the microplate is sucked away to complete the liquid discharge action.

[0067] The working process of the plate washer 200 and the liquid path system 300 to realize magnetic bead purification is as follows:

[0068] Place the 96-well microplate on the magnetic plate 212. The elastic clamping member 213 clamps the microplate tightly. The first oscillating motor 221 drives the microplate to oscillate back and forth through the screw-nut mechanism. After the oscillation action is completed, all the magnetic beads in the reaction solution in the microplate are adsorbed on the bottom of the microplate. Then, the first liquid injection motor 241 drives the liquid injection block 231 to move in the X direction. The microplate is arranged in a 12×8 pattern, with 8 micro-wells in a column. First, align the liquid injection needle 232 and the liquid discharge needle 233 with the holes in the first column in the X direction. Then, the second liquid injection motor 245 drives the liquid injection block 231 to move downward. At this time, the vacuum pump is turned on. During the downward movement of the liquid injection needle 232, it sucks liquid while descending. The reaction solution is discharged through the liquid discharge needle 233 by the suction force generated by the vacuum pump, and all the upper-layer reaction solution in the microplate is sucked away. Then, the second liquid injection motor 245 rises to the height before the descent, and the two-position three-way solenoid valve 305 switches to the closed state. The plunger pump 304 starts to suck liquid, sucking the cleaning liquid in the cleaning liquid bottle 301 into the plunger pump 304. After completion, start the liquid injection action of the plunger pump 304, and inject the cleaning liquid in the plunger pump 304 into the microplate through the pipeline, the liquid injection tank 2313, and the liquid injection needle 232, completing the liquid injection action for one column. Repeat the above operations for the subsequent 11 columns of the microplate to complete the liquid injection action of the cleaning liquid. Then, perform the oscillation action again, and then perform the liquid suction and discharge. Repeat several times to complete the purification of the magnetic beads. During the liquid discharge process of the liquid injection block 231, the two-position two-way solenoid valve 306 (normally open) is in the closed state. Each time the liquid injection is completed, the two-position two-way solenoid valve 306 is opened to close the passage to prevent liquid dripping and contaminating the sample.

[0069] Continue to refer to Figures 9 to 11 As shown, the oscillation incubator 400 further includes a mounting table 440. In some embodiments, the oscillation driving assembly 420 includes a second oscillation motor 421, an eccentric structure 422, and an oscillation bottom plate 423. The second oscillation motor 421 is installed in the mounting table 440. The eccentric structure 422 includes an eccentric bottom plate 4221 and an eccentric shaft 4222. The eccentric shaft 4222 is eccentrically arranged on the eccentric bottom plate 4221. The motor shaft of the second oscillation motor 421 is connected to the eccentric bottom plate 4221. A bearing 4231 is provided on the oscillation bottom plate 423, and the eccentric shaft 4222 is rotatably connected in the bearing 4231. The oscillation bottom plate 423 is connected to the reagent plate 410.

[0070] In some embodiments, the oscillation bottom plate 423 is disposed on the tabletop of the mounting table 440. A guiding mechanism 450 is provided on the mounting table 440, and guiding and limiting holes 4232 are provided on the oscillation bottom plate 423. As Figure 12As shown, the guide mechanism 450 includes a positioning pin 451 protruding from the table, and an elastic ring 452 and a roller 453 sleeved on the positioning pin 451. The radial dimension of the elastic ring 452 is larger than the radial dimension of the roller 453, and the elastic ring 452 and the roller 453 are movably arranged in the guide limit hole 4232. The cooperation between the guide mechanism 450 and the guide limit hole 4232 can ensure the uniqueness of the direction of the oscillation bottom plate 423 during the oscillation process.

[0071] In some embodiments, a plurality of support rods 460 are provided on the mounting platform 440, and a support hole 4233 is provided on the bottom surface of the oscillating bottom plate 423. The top of the support rod 460 is inserted into the support hole 4233 without affecting the oscillation of the oscillating bottom plate 423 in the horizontal plane, and only supports the oscillating bottom plate 423 in the vertical direction, and ensures that the height difference between the oscillating bottom plate 423 and the mounting platform 440 surface in the vertical direction meets the requirements. In a specific embodiment, four support rods 460 are provided, and the four support rods 460 are evenly distributed around the circumference of the eccentric structure 422, and the four support rods 460 are inserted into the four support holes 4233 one by one.

[0072] In some embodiments, a plurality of positioning blocks 411 are provided on the top surface of the oscillating bottom plate 423 along the circumferential direction, and the plurality of positioning blocks 411 are used to limit the position of the reagent plate 410. In one embodiment, the oscillating bottom plate 423 is a rectangular plate, and four positioning blocks 411 are provided, and the positioning blocks 411 are L-shaped, and the four positioning blocks 411 are respectively provided at the corners of the oscillating bottom plate 423.

[0073] In some embodiments, the heating assembly 430 includes a heating base plate 431, a heating film 432, a temperature sensor 433 and a temperature control protection switch 434. The heating base plate 431 is stacked below the reagent plate 410, and a heating film 432 is provided on the heating base plate 431, and the heating film 432 is used to realize the heating of the heating base plate 431, and the heating base plate 431 is used to transfer heat to the reagent plate 410. The temperature sensor 433 is used to detect the heating temperature of the heating film 432, and the heating film 432 and the temperature sensor 433 constitute a temperature control closed loop control, and the constant temperature incubation of the reaction reagent in the reagent plate 410 can be realized by board control. The temperature control protection switch 434 can be powered off to protect the heating film 432 when the heating film 432 is out of control, thereby improving the safety of the entire oscillation incubator 400. It should be noted that the oscillation incubator 400 can be provided with multiple and integrated together.

[0074] In some embodiments, a transfer circuit board 470 and a motor control board 480 are also connected to the side panels of the mounting platform 440 .

[0075] In some embodiments, an optocoupler 491 is further provided on the tabletop of the mounting table 440, and an optocoupler baffle 492 is provided on the eccentric base plate 4221. The cooperation of the optocoupler 491 and the optocoupler baffle 492 can achieve the control of the oscillation times.

[0076] The working principle of the oscillating incubator 400 is as follows: The second oscillating motor 421 drives the eccentric structure 422 to rotate around the central axis of the eccentric base plate 4221. The central axis of the eccentric shaft 4222 is parallel and spaced from the central axis of the eccentric base plate 4221. The eccentric shaft 4222 extends into the bearing 4231 of the oscillating bottom plate 423, and the eccentric base plate 4221 is supported on the mounting table 440 by a plurality of support rods 460 to achieve vertical support and limit. The oscillating bottom plate 423 and the mounting table 440 are limited in the horizontal plane through the guiding mechanism 450 and the guiding and limiting holes 4232. Thus, under the drive of the second oscillating motor 421, the oscillating bottom plate 423 performs an oscillating motion in the specified XY plane direction. The amplitude of the oscillation of the oscillating bottom plate 423 can be 3 mm, and the motor shaft of the second oscillating motor 421 can rotate at a high speed of up to 2000 r / min. Moreover, by controlling the heating film 432, the heating of the heating bottom plate 431 can be achieved. The heating bottom plate 431 transfers heat to the reagent plate 410 on the oscillating bottom plate 423. The heating film 432 and the temperature sensor 433 form a temperature control closed-loop system, and the reagent can be incubated at a constant temperature through board card control. Based on the above, the oscillating incubator 400 can complete the stable oscillation and constant temperature incubation actions of the reagent plate 410.

[0077] The demagnetizer 500 is used to demagnetize the magnetic beads. The specific structure of the demagnetizer 500 is prior art and will not be described in detail here. The demagnetizer 500 is controlled by the I0 board of the software to open and close the demagnetizer 500. After opening, the sample with magnetic beads can be manually placed 5 mm - 20 mm above the demagnetizer 500. The demagnetization operation of the magnetic beads can be completed by quickly moving the sample about 25 times in the length direction. After the demagnetization operation is completed, the demagnetizer 500 is closed through the I0 of the software to complete the operation.

[0078] It should be noted that after passing through the "oscillating incubator 400", the "plate washer 200", and the "demagnetizer 500", the sample preparation is completed by adding liquid through a pipette or a liquid transfer workstation. The sample can be directly loaded onto the optical machine for detection to obtain the experimental results. In some embodiments, the medical device further includes an optical machine, which can be integrated on the electric control box 100 and share at least part of a set of power supply components and communication components with the oscillating incubator 400, the plate washer 200, and the demagnetizer 500.

[0079] Such as Figure 13As shown in the figure, the power supply component includes a socket, a switch, a filter, a 220V terminal block, a 24V switching power supply, and a 24V terminal block. Among them, the socket is used to externally connect a 220V power supply. After passing through the switch and the filter, the socket is branched at the 220V terminal block to connect a degausser 500 and a 24V switching power supply. The 24V switching power supply is then branched at the 24V terminal block to connect a CAN server, a serial server, a switch, a temperature control board, an oscillation controller, and a circuit board washer 200, thus completing the power supply to the entire medical device.

[0080] As Figure 14 shown in the figure, the communication component includes a PC, a switch, a CAN server, and a serial server. Among them, after the PC is connected to the switch, the switch is branched into two paths to connect the CAN server and the serial server respectively. The CAN server is further branched into two paths to connect the temperature control board control and the IO board control, while the serial server is branched into two paths to connect the circuit board washer 200 control and the oscillation motor control.

[0081] In this embodiment, the control mechanism for implementing control can be a centralized or distributed controller. For example, the controller can be a single separate microcontroller or composed of multiple distributed microcontrollers. A control program can run in the microcontroller to control each component to implement its functions.

[0082] Obviously, the above embodiments of the present invention are merely examples for clearly illustrating the present invention, rather than limitations on the implementation manners of the present invention. For those of ordinary skill in the art, other different forms of changes or modifications can be made based on the above description. It is not necessary and impossible to enumerate all the implementation manners here. Any modifications, equivalent replacements, and improvements made within the spirit and principle of the present invention shall be included within the protection scope of the claims of the present invention.

Claims

1. A medical device, characterized in that, include: An electric control box (100), wherein a power supply component and a communication component are arranged in the electric control box (100); A plate washer (200), the plate washer (200) comprising a magnetic plate seat (210), an oscillating assembly (220) and a liquid injection assembly, the magnetic plate seat (210) being used to mount a liquid injection plate, the oscillating assembly (220) being used to drive the magnetic plate seat (210) to oscillate in a horizontal direction, and the liquid injection assembly being used to inject liquid into the liquid injection plate or to absorb liquid from the liquid injection plate; A liquid circuit system (300), the liquid circuit system (300) being in communication with the liquid injection assembly and being used for injecting liquid into the liquid injection assembly and for drawing liquid from the liquid injection assembly; An oscillating incubator (400), the oscillating incubator (400) comprising a reagent plate (410), an oscillating drive component (420) and a heating component (430), the oscillating drive component (420) being transmission-connected to the reagent plate (410) and used to drive the reagent plate (410) to oscillate in a horizontal plane, and the heating component (430) being used to heat the reagent plate (410); A demagnetizer (500), the demagnetizer (500) being used to demagnetize magnetic beads in the liquid injection plate placed thereon; Wherein, the plate washer (200), the oscillating incubator (400) and the demagnetizer (500) are at least partially integrated on the electrical control box (100), and the plate washer (200), the oscillating incubator (400) and the demagnetizer (500) share a set of the power supply components and a set of the communication components.

2. The medical device according to claim 1, characterized in that The magnetic plate seat (210) comprises a mounting seat (211), a magnetic plate (212) and an elastic clamping piece (213); the mounting seat (211) is provided with a mounting cavity, the magnetic plate (212) is arranged in the mounting cavity, and the elastic clamping piece (213) is used for clamping the liquid injection plate placed on the magnetic plate (212).

3. The medical device according to claim 1, characterized in that The oscillation assembly (220) comprises a first oscillation motor (221), a first screw rod (222), a first nut block (223) and a first connecting block (224); the motor shaft of the first oscillation motor (221) is rotatably connected to one end of the first screw rod (222) and is capable of driving the first screw rod (222) to rotate; the first nut block (223) is threadedly connected to the first screw rod (222); and the first connecting block (224) is connected between the first nut block (223) and the magnetic plate seat (210).

4. The medical device according to claim 1, characterized in that The liquid injection assembly includes a liquid injection mechanism (230) and a liquid injection driving mechanism (240). The liquid injection mechanism (230) is movably arranged above the magnetic plate seat (210). The liquid injection driving mechanism (240) is in transmission connection with the liquid injection mechanism (230) and is used to drive the liquid injection mechanism (230) to approach or move away from the liquid injection plate.

5. The medical device according to claim 1, wherein the liquid injection assembly includes a liquid injection mechanism (230). The liquid injection mechanism (230) is movably arranged above the magnetic plate seat (210) and is used to inject liquid into the liquid injection plate or suck liquid from the liquid injection plate; the liquid injection mechanism (230) includes a liquid injection block (231), a liquid injection needle (232) and a liquid discharge needle (233). A liquid injection groove (2313) and a liquid discharge groove (2314) are arranged in the liquid injection block (231). The liquid injection groove (2313) is respectively communicated with the liquid injection needle (232) and the liquid injection pipeline of the liquid path system (300). The liquid discharge groove (2314) is respectively communicated with the liquid discharge needle (233) and the liquid discharge pipeline of the liquid path system (300). And the liquid injection port of the liquid injection needle (232) is higher than the liquid discharge port of the liquid discharge needle (233).

6. The medical device according to claim 4, wherein the liquid injection driving mechanism (240) includes a first liquid injection motor (241), a liquid injection synchronous belt (242), a connection structure, a second liquid injection motor (245), a second lead screw and a second nut block. The motor shaft of the first liquid injection motor (241) is connected with the connection structure through the liquid injection synchronous belt (242) and can drive the connection structure to move in the horizontal direction. The second liquid injection motor (245) is arranged on the connection structure. The motor shaft of the second liquid injection motor (245) is in transmission connection with one end of the second lead screw and can drive the second lead screw to rotate around the vertical axis. The second nut block is threadedly connected to the second lead screw, and the second nut block is connected with the liquid injection mechanism (230).

7. The medical device according to claim 1, wherein the liquid injection assembly includes a liquid injection mechanism (230). The liquid injection mechanism (230) is movably arranged above the magnetic plate seat (210) and is used to inject liquid into the liquid injection plate or suck liquid from the liquid injection plate; The liquid path system (300) includes a cleaning liquid bottle (301), a distilled water bottle (302), a waste liquid bottle (303), a plunger pump (304), a two-position three-way solenoid valve (305), and a two-position two-way solenoid valve (306). The cleaning liquid bottle (301) is communicated with the first liquid inlet of the two-position three-way solenoid valve (305), the distilled water bottle (302) is communicated with the second liquid inlet of the two-position three-way solenoid valve (305), the liquid outlet port of the two-position three-way solenoid valve (305) is communicated with the plunger pump (304) through a first branch pipe (307), the liquid inlet port of the two-position two-way solenoid valve (306) is communicated with the plunger pump (304) through a second branch pipe (308), the liquid outlet port of the two-position two-way solenoid valve (306) is communicated with the liquid inlet of the liquid injection mechanism (230), and the liquid outlet of the liquid injection mechanism (230) is communicated with the waste liquid bottle (303) through a waste liquid pipe (311).

8. The medical device according to claim 7, wherein the liquid injection mechanism (230) includes two spaced liquid outlets. The waste liquid pipe (311) is a three-way pipeline. The two branch pipes of the waste liquid pipe (311) are respectively communicated with the two liquid outlets, and the main pipe of the waste liquid pipe (311) is communicated with the waste liquid bottle (303).

9. The medical device according to claim 1, wherein the oscillation driving assembly (420) includes a second oscillation motor (421), an eccentric structure (422), and an oscillation bottom plate (423). The eccentric structure (422) includes an eccentric bottom plate (4221) and an eccentric shaft (4222). The eccentric shaft (4222) is eccentrically arranged on the eccentric bottom plate (4221). The motor shaft of the second oscillation motor (421) is connected to the eccentric bottom plate (4221). A bearing (4231) is arranged on the oscillation bottom plate (423). The eccentric shaft (4222) is rotatably connected in the bearing (4231). The oscillation bottom plate (423) is connected to the reagent plate (410).

10. The medical device according to claim 9, wherein the oscillation incubator (400) further includes a mounting table (440). The oscillation bottom plate (423) is arranged on the tabletop of the mounting table (440). A guiding mechanism (450) is arranged on the mounting table (440). A guiding and limiting hole (4232) is arranged on the oscillation bottom plate (423). The guiding mechanism (450) includes a positioning pin (451) protruding from the tabletop, an elastic ring (452), and a roller (453) sleeved on the positioning pin (451). The radial dimension of the elastic ring (452) is larger than the radial dimension of the roller (453), and the elastic ring (452) and the roller (453) are movably arranged in the guiding and limiting hole (4232).

11. The medical device according to claim 1, wherein The heating component (430) includes a heating bottom plate (431), a heating film (432), a temperature sensor (433) and a temperature control protection switch (434). The heating bottom plate (431) is stacked below the reagent plate (410). The heating film (432) is arranged on the heating bottom plate (431). The temperature sensor (433) is used to detect the heating temperature of the heating film (432), and the temperature control protection switch (434) is used to cut off the power supply to protect the heating film (432).

12. The medical device according to claim 1, wherein The plate washer (200), the oscillating incubator (400) and the demagnetizer (500) are all arranged on the electric control box (100), and the oscillating incubator (400) and the demagnetizer (500) are arranged at intervals in a first direction, and the plate washer (200) is arranged side by side with the oscillating incubator (400) and the demagnetizer (500) in a second direction.

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