Multi-depth-of-field imaging bacteria detection microscope

By designing a multi-deep field imaging bacterial detection microscope, using spectroscopic detection mechanism and multi-deep field camera, the existing microscopes have solved the problem of unclear imaging and difficulty in bacterial detection, and achieved the effect of multi-deep field imaging and image fusion output.

CN120195860APending Publication Date: 2025-06-24HUIZHOU HAOYUAN OPTICAL TECH CO LTD +1
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Patent Information

Application Number
CN202510607857.8
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2025-05-13
Publication Date
2025-06-24

AI Technical Summary

Technical Problem

Existing microscopes are susceptible to light during bacterial detection, resulting in unclear imaging, and are inconvenient to adjust the microscopic imaging effect, and cannot integrate acquired images.

Method used

A multi-deep field imaging bacterial detection microscope is designed, using adjustment mechanisms and spectroscopic detection mechanisms to realize layered light processing and fusion output of images through components such as lenses, light-grabbing chips, lens barrels and multi-deep field cameras.

Benefits of technology

Multi-depth of field imaging of bacteria is achieved, which can adjust light to improve imaging clarity, and integrate different depth of field images through multi-depth of field cameras to meet the needs of microscopic imaging detection of different degrees.

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Abstract

The invention discloses a multi-depth-of-field imaging bacteria detection microscope, which comprises an adjusting mechanism and a light splitting detection mechanism, a shielding box is installed on the outer side of the adjusting mechanism, the light splitting detection mechanism is installed at the front end of the adjusting mechanism, the light splitting detection mechanism comprises a transmission frame, two light harvesting chips are fixedly installed at the front end of the transmission frame, and the light harvesting chips are arranged on the transmission frame. The front ends of the two light harvesting chips are slidably connected with a first lens cone and a second lens cone, a mainboard is fixedly installed on one side of the first lens cone and one side of the second lens cone, a multi-depth-of-field camera is arranged in the mainboard, the first lens cone is located above the second lens cone, and a second light guide base is installed at the front end of the second lens cone. According to the invention, through layering and twice reflection of bacteria imaging light, twice microscopic imaging of bacteria can be realized conveniently, the microscopic imaging effect can be adjusted conveniently, imaging is more accurate, and fusion and integration of acquired images with different depth of field can be realized.
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Description

Technical Field

[0001] The present invention relates to the technical field of microscopes, and particularly to a multi-depth-of-field imaging bacteria detection microscope. Background Technique

[0002] The principle of a microscope is mainly based on the optical principle, and a microscopic object is magnified and imaged through a lens system. A microscope usually consists of an eyepiece, an objective lens, a stage and a reflector. Both the eyepiece and the objective lens are convex lenses. The objective lens has a shorter focal length and is used to magnify the object and form an inverted real image, while the eyepiece magnifies this real image again and forms an upright virtual image. Finally, the human eye can observe the magnified object image. A microscope can magnify microscopic objects that cannot be seen by the naked eye, such as bacteria, cells, etc., to help scientists and researchers study these microscopic structures in detail.

[0003] When the existing microscope detects bacteria, it is easily affected by light, resulting in unclear imaging of bacteria, and it is not convenient to adjust the microscopic imaging effect of bacteria. At the same time, it cannot integrate the acquired images. Therefore, it does not meet the existing requirements. For this reason, we propose a multi-depth-of-field imaging bacteria detection microscope. Summary of the Invention

[0004] The purpose of the present invention is to provide a multi-depth-of-field imaging bacteria detection microscope to solve the problems in the above background technique that when the existing microscope detects bacteria, it is easily affected by light, resulting in unclear imaging of bacteria, and it is not convenient to adjust the microscopic imaging effect of bacteria.

[0005] To achieve the above purpose, the present invention provides the following technical solution: A multi-depth-of-field imaging bacteria detection microscope, including an adjustment mechanism and a spectroscopic detection mechanism. A shielding box is installed outside the adjustment mechanism, and a spectroscopic detection mechanism is installed at the front end of the adjustment mechanism. The spectroscopic detection mechanism includes a transmission frame. Two light-catching chips are fixedly installed at the front end of the transmission frame. A first lens barrel and a second lens barrel are slidably connected to the front ends of the two light-catching chips. A main board is fixedly installed on one side of the first lens barrel and the second lens barrel. The main board is internally provided with a multi-depth-of-field camera. The first lens barrel is located above the second lens barrel. A second light guide seat is installed at the front end of the second lens barrel. A reflecting lens is installed on the lower end surface of the second light guide seat. A first light guide seat is installed at the front end of the first lens barrel. A spectroscopic lens is installed on the lower end surface of the first light guide seat. A lens is installed at the upper end of the first light guide seat.

[0006] Preferably, the adjustment mechanism includes a stepping motor. The output end of the stepping motor is connected to a transmission screw through a coupling. A push plate is installed outside the transmission screw. A guide frame is slidably connected to the outside of the push plate.

[0007] Preferably, the shielding box includes a protective seat, a light source holder is fixedly installed at the front end of the protective seat, a mounting seat is fixedly installed at the upper end of the protective seat, and a load placing surface is provided at the front part of the upper end surface of the mounting seat.

[0008] Preferably, the stepping motor is fixedly connected to the protective seat, the transmission screw is threadedly connected to the push plate, one end of the push plate penetrates through the guide frame and is fixedly connected to the rear end of the transmission frame, and the guide frame is fixedly connected to the mounting seat.

[0009] Preferably, the first lens barrel and the second lens barrel are coaxial with the two light capturing chips, the main board is electrically connected to the stepping motor and the two light capturing chips, the length dimension of the first lens barrel is greater than the length dimension of the second lens barrel, and the front ends of the first lens barrel and the second lens barrel are fixedly connected to the protective seat through a first light guide seat and a second light guide seat respectively.

[0010] Preferably, the bottom end of the lens is threadedly connected to the first light guide seat, a through hole is provided in the middle of the load placing surface, the upper end of the lens is inserted into the inner side of the through hole, the beam splitting lens is inserted between the first light guide seat and the second light guide seat, the beam splitting lens is fixedly connected to the first light guide seat and the reflecting lens is fixedly connected to the second light guide seat, and the lens is perpendicular to the axes of the first lens barrel and the second lens barrel.

[0011] Compared with the prior art, the beneficial effects of the present invention are as follows:

[0012] 1. The present invention can filter the light irradiating the specimen slide through the lens and guide it to the inside of the first light guide seat. The beam splitting lens can reflect and transmit the light transmitted by the lens, thereby realizing the hierarchical processing of light. The lens is perpendicular to the first lens barrel and the second lens barrel, so that the light reflected by the beam splitting lens is transmitted to one of the light capturing chips through the first lens barrel. The reflecting lens can reflect the layered light and transmit it to the other light capturing chip through the second lens barrel.

[0013] 2. The present invention can receive the two layered light beams through the two light capturing chips and convert them into image information and transmit it to the main board. The main board adjusts the positions of the light capturing chips according to the clarity of the images. The stepping motor drives the push plate to linearly reciprocate under the guiding action of the guide frame through the transmission screw. Then the push plate synchronously drives the two light capturing chips to slide and expand relative to the first lens barrel and the second lens barrel through the transmission frame, realizing the adjustment of the distances between the beam splitting lens and the reflecting lens and the two light capturing chips, meeting the detection operations of microscopic imaging of bacteria at different levels. A multi-depth-of-field camera is built into the main board, enabling the fusion output of different depth-of-field images collected. BRIEF DESCRIPTION OF THE DRAWINGS

[0014] Figure 1 is a schematic structural diagram of the whole of the present invention;

[0015] Figure 2 Explosion structure schematic diagram of the shielding box of the present invention;

[0016] Figure 3 Structure schematic diagram of the adjusting mechanism of the present invention;

[0017] Figure 4 Explosion structure schematic diagram of the spectroscopic detection mechanism of the present invention.

[0018] In the figure: 1. Shielding box; 101. Protection seat; 102. Mounting seat; 103. Light source holder; 104. Object placing surface; 2. Adjusting mechanism; 201. Stepper motor; 202. Transmission screw; 203. Guide frame; 204. Push plate; 3. Spectroscopic detection mechanism; 301. First lens barrel; 302. Second lens barrel; 303. Lens; 304. First light guide seat; 305. Second light guide seat; 306. Light capturing chip; 307. Transmission frame; 308. Main board; 309. Spectroscopic lens; 310. Reflective lens. Detailed implementation manners

[0019] Next, the technical solutions in the embodiments of the present invention will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments.

[0020] Please refer to Figure 1 and Figure 2 An embodiment provided by the present invention: A multi-depth-of-field imaging bacteria detection microscope includes an adjusting mechanism 2 and a spectroscopic detection mechanism 3. A shielding box 1 is installed outside the adjusting mechanism 2. The shielding box 1 includes a protection seat 101. A light source holder 103 is fixedly installed at the front end of the protection seat 101. A mounting seat 102 is fixedly installed at the upper end of the protection seat 101. An object placing surface 104 is provided at the front part of the upper end surface of the mounting seat 102. The protection seat 101 and the mounting seat 102 facilitate the shielding installation of the adjusting mechanism 2 and the spectroscopic detection mechanism 3, improving the microscopic imaging effect.

[0021] Please refer to Figure 3 and Figure 4, a spectroscopic detection mechanism 3 is installed at the front end of the adjustment mechanism 2. The spectroscopic detection mechanism 3 includes a transmission frame 307. Two light-catching chips 306 are fixedly installed at the front end of the transmission frame 307. A first lens barrel 301 and a second lens barrel 302 are slidably connected to the front ends of the two light-catching chips 306. The length dimension of the first lens barrel 301 is greater than that of the second lens barrel 302. The first lens barrel 301 and the second lens barrel 302 are coaxial with the two light-catching chips 306. A main board 308 is fixedly installed on one side of the first lens barrel 301 and the second lens barrel 302. A multi-depth-of-field camera is built into the main board 308, enabling the fusion output of different depth-of-field images collected;

[0022] The first lens barrel 301 is located above the second lens barrel 302. A second light guide seat 305 is installed at the front end of the second lens barrel 302. A first light guide seat 304 is installed at the front end of the first lens barrel 301. The front ends of the first lens barrel 301 and the second lens barrel 302 are fixedly connected to the protective seat 101 through the first light guide seat 304 and the second light guide seat 305 respectively. The first lens barrel 301 and the second lens barrel 302 can synchronously transmit the stratified light;

[0023] A reflecting lens 310 is installed on the lower end face of the second light guide seat 305. A spectroscopic lens 309 is installed on the lower end face of the first light guide seat 304. The spectroscopic lens 309 is inserted between the first light guide seat 304 and the second light guide seat 305. The spectroscopic lens 309 is fixedly connected to the first light guide seat 304, and the reflecting lens 310 is fixedly connected to the second light guide seat 305. A lens 303 is installed at the upper end of the first light guide seat 304. The bottom end of the lens 303 is threadedly connected to the first light guide seat 304. A through hole is provided in the middle of the object placement surface 104. The upper end of the lens 303 is inserted into the inner side of the through hole. The lens 303 is perpendicular to the axes of the first lens barrel 301 and the second lens barrel 302. The spectroscopic lens 309 can reflect and transmit the light transmitted by the lens 303, thereby realizing the stratification processing of the light, and the reflecting lens 310 can reflect the stratified light.

[0024] Please refer to Figures 2 to 4, the adjustment mechanism 2 includes a stepper motor 201. The main board 308 is electrically connected to the stepper motor 201 and two light-capturing chips 306. The stepper motor 201 is fixedly connected to the protective seat 101. The output end of the stepper motor 201 is connected with a transmission screw 202 through a coupling. A push plate 204 is installed on the outer side of the transmission screw 202. The transmission screw 202 is threadedly connected with the push plate 204. The outer side of the push plate 204 is slidably connected with a guide frame 203. One end of the push plate 204 penetrates through the guide frame 203 and is fixedly connected to the rear end of the transmission frame 307. The guide frame 203 is fixedly connected to the mounting seat 102. The stepper motor 201 drives the two light-capturing chips 306 to slide synchronously through the transmission screw 202, the push plate 204 and the transmission frame 307, so as to realize the adjustment of the distance between the beam-splitting lens 309 and the reflecting lens 310 and the two light-capturing chips 306, and meet the detection operation of microscopic imaging of bacteria at different levels.

[0025] During use, the adjustment mechanism 2 and the beam-splitting detection mechanism 3 are installed inside the protective seat 101 and the mounting seat 102. When performing microscopic detection on bacteria, a specimen slide containing the bacteria to be detected is placed on the specimen placement surface 104. The power is turned on, and the specimen slide is irradiated by the light source on the light source holder 103. There is a through hole in the middle of the specimen placement surface 104. The upper end of the lens 303 is inserted into the inside of the through hole, so that the light irradiating the specimen slide can be filtered by the lens 303 and guided into the inside of the first light guide seat 304;

[0026] A beam-splitting lens 309 is fixedly installed on the lower end surface of the first light guide seat 304, so that the light transmitted by the lens 303 can be reflected and transmitted through the beam-splitting lens 309, thereby realizing the hierarchical processing of the light. The lens 303 is perpendicular to the first lens barrel 301 and the second lens barrel 302, so that the light reflected by the beam-splitting lens 309 is transmitted to one of the light-capturing chips 306 through the first lens barrel 301. A reflecting lens 310 is fixedly installed on the lower end surface of the second light guide seat 305, so that the stratified light can be reflected by the reflecting lens 310 and transmitted to the other light-capturing chip 306 through the second lens barrel 302;

[0027] The main board 308 is electrically connected to the stepper motor 201 and the two light-capturing chips 306, so that the two light-capturing chips 306 can receive the stratified two-way light and convert it into image information and transmit it to the main board 308. The main board 308 adjusts the position of the light-capturing chip 306 according to the clarity of the image;

[0028] Specifically, the stepper motor 201 drives the push plate 204 to linearly reciprocate under the guiding of the guiding frame 203 by means of the transmission screw rod 202 under the supporting action of the protective seat 101. Furthermore, the push plate 204 synchronously drives the two light capturing chips 306 to slide and expand relative to the first lens barrel 301 and the second lens barrel 302 through the transmission frame 307, so as to realize the adjustment of the distances between the beam splitting lens 309 and the reflecting lens 310 and the two light capturing chips 306, and meet the detection operation of microscopic imaging of bacteria at different levels. A multi-depth camera is built in the main board 308, so that different depth-of-field images collected during the adjustment of the light capturing chip 306 can be fused and output through the multi-depth camera.

[0029] For those skilled in the art, it is obvious that the present invention is not limited to the details of the above exemplary embodiments, and the present invention can be implemented in other specific forms without departing from the spirit or basic characteristics of the present invention. Therefore, from any point of view, the embodiments should be regarded as exemplary and non-limiting. The scope of the present invention is defined by the appended claims rather than the above description. Therefore, all changes falling within the meaning and scope of the equivalent elements of the claims are intended to be included in the present invention. Any reference signs in the claims should not be construed as limiting the claimed invention.

Claims

1. A multi-depth imaging bacteria detection microscope, comprising an adjustment mechanism (2) and a spectroscopic detection mechanism (3), characterized in that: A shielding box (1) is installed on the outer side of the adjustment mechanism (2); a spectroscopic detection mechanism (3) is installed at the front end of the adjustment mechanism (2); the spectroscopic detection mechanism (3) comprises a transmission frame (307); two light-capturing chips (306) are fixedly installed at the front end of the transmission frame (307); the front ends of the two light-capturing chips (306) are slidably connected to a first lens barrel (301) and a second lens barrel (302); a main board (308) is fixedly installed on one side of the first lens barrel (301) and the second lens barrel (302); The board (308) has a built-in multi-depth camera, the first lens barrel (301) is located above the second lens barrel (302), the front end of the second lens barrel (302) is equipped with a second light guide seat (305), the lower end surface of the second light guide seat (305) is equipped with a reflective lens (310), the front end of the first lens barrel (301) is equipped with a first light guide seat (304), the lower end surface of the first light guide seat (304) is equipped with a beam splitter lens (309), and the upper end of the first light guide seat (304) is equipped with a lens (303).

2. The multi-depth imaging bacteria detection microscope according to claim 1, characterized in that: The adjustment mechanism (2) comprises a stepper motor (201), the output end of the stepper motor (201) is connected to a transmission screw (202) via a coupling, a push plate (204) is installed on the outer side of the transmission screw (202), and a guide frame (203) is slidably connected to the outer side of the push plate (204).

3. The multi-depth imaging bacteria detection microscope according to claim 2, characterized in that: The shielding box (1) comprises a protection seat (101), a light source frame (103) is fixedly mounted on the front end of the protection seat (101), a mounting seat (102) is fixedly mounted on the upper end of the protection seat (101), and a loading surface (104) is provided at the front of the upper end surface of the mounting seat (102).

4. The multi-depth imaging bacteria detection microscope according to claim 3, characterized in that: The stepper motor (201) is fixedly connected to the protective seat (101), the transmission screw (202) is threadedly connected to the push plate (204), one end of the push plate (204) passes through the guide frame (203) and is fixedly connected to the rear end of the transmission frame (307), and the guide frame (203) is fixedly connected to the mounting seat (102).

5. The multi-depth imaging bacteria detection microscope according to claim 4, characterized in that: The first lens barrel (301) and the second lens barrel (302) are coaxial with the two light-capturing chips (306); the main board (308) is electrically connected to the stepping motor (201) and the two light-capturing chips (306); the length of the first lens barrel (301) is greater than the length of the second lens barrel (302); and the front ends of the first lens barrel (301) and the second lens barrel (302) are fixedly connected to the protective seat (101) via the first light guide seat (304) and the second light guide seat (305), respectively.

6. The multi-depth imaging bacteria detection microscope according to claim 5, characterized in that: The bottom end of the lens (303) is connected to the first light guide seat (304) by means of a thread, a through hole is provided in the middle of the object placement surface (104), the top end of the lens (303) is plugged into the inner side of the through hole, the beam splitter lens (309) is plugged into between the first light guide seat (304) and the second light guide seat (305), the beam splitter lens (309) and the first light guide seat (304) and the reflective lens (310) and the second light guide seat (305) are all fixedly connected, and the lens (303) is perpendicular to the axes of the first lens barrel (301) and the second lens barrel (302).