Automatic inoculation device for tremella aurantialba liquid strain and inoculation method thereof

Abstract

The invention discloses a tremella aurantialba liquid strain automatic inoculation device and an inoculation method thereof.The inoculation device comprises an inoculation needle which is connected to a supporting rod in a liftable mode, a tremella aurantialba liquid strain can be contained in the inoculation needle, an inoculation hole is formed in the side face, close to the tail end of a needle head of the inoculation needle, of the inoculation needle, and a leakage piece is rotatably connected to the top end of the inoculation hole; the leakage sheet can close the inoculation hole; a flow guide inclined plane is arranged in the inoculating needle, and the bottom end of the flow guide inclined plane is propped against and fixed with the bottom end of the inoculating hole; the inoculation ball is adjacently arranged beside the inoculation needle, and the inoculation ball is connected to the supporting rod in a lifting mode; the conveying belt is used for placing fungus bags, and the fungus bags can be conveyed to the positions below the inoculation needles and the inoculation balls by the conveying belt and are automatically inoculated under the cooperation of the inoculation needles and the inoculation balls. According to the invention, batch and automatic injection work of the tremella aurantialba bacterial liquid is realized.

Description

Technical Field

[0001] The invention relates to the field of auricularia auricularia liquid strain inoculation, and in particular to an automatic auricularia auricularia liquid strain inoculation device and an inoculation method thereof. Background Art

[0002] Golden ear is a high-value edible and medicinal fungus. Its current cultivation mode usually adopts liquid strain inoculation technology to improve the mycelium growth rate and cultivation efficiency. The traditional golden ear liquid strain inoculation process is: inoculate the culture bottle mother bell into the liquid culture medium, obtain the liquid strain by shaking bottle or fermentation culture, and then artificially inoculate the liquid strain into the solid matrix, and finally cultivate the golden ear fruiting body in the factory. Golden ear cultivation adopts the method of artificial injection of liquid strain, which is labor-intensive, inefficient and easy to pollute.

[0003] After searching, Chinese patent document CN118542191A discloses an automatic injection device for golden ear cultivation, which relates to the field of golden ear cultivation technology, including: a base, on which a storage component is arranged, and the storage component is used to store liquid golden ear fungus; a liquid supply component, which is fixed on the base; the liquid supply component includes: a cylinder, which is fixed on the base; a first piston, which is slidably arranged in the cylinder, and a hollow shaft is arranged on one side of the first piston. In the process of injection, the injection component can use the high-pressure gas provided by the gas storage component to lift the fungus bag so that the fungus bag and the extrusion seat in the injection component collide to form a small pit, and can also use the negative pressure environment provided by the gas storage component to separate the plastic film and the culture medium at the small pit of the fungus bag, and at the same time, the injection component injects bacteria into the small pit, thereby realizing the automatic injection of golden ear fungus liquid.

[0004] Although CN118542191A realizes automated bacterial injection, the inventor's invention concept is different from the prior art and adopts completely different technical means. Summary of the invention

[0005] In view of this, in order to solve the above-mentioned technical problems, the purpose of the present invention is to propose an automatic inoculation device for golden ear liquid fungus and an inoculation method thereof, which adopts different inventive concepts to realize the injection of golden ear fungus liquid in a batch and automated manner.

[0006] The technical solutions adopted are:

[0007] The invention discloses an automatic inoculation device for Tremella fuciformis liquid bacteria, comprising:

[0008] An inoculation needle is connected to a rod in a liftable manner. A liquid fungus of Tremella fuciformis can be loaded inside the inoculation needle. An inoculation hole is provided on the side near the end of the needle head of the inoculation needle. A leak sheet can be rotatably connected to the top of the inoculation hole, and the leak sheet can close the inoculation hole. A guide slope is provided inside the inoculation needle, and the bottom end of the guide slope is fixed against the bottom end of the inoculation hole.

[0009] An inoculation ball, which is arranged adjacent to the inoculation needle and is liftably connected to the support rod;

[0010] The conveyor belt is used for placing the fungus bag, which can be conveyed by the conveyor belt to the bottom of the inoculation needle and the inoculation ball, and automatically inoculated with the cooperation of the inoculation needle and the inoculation ball.

[0011] Furthermore, the inoculation needle is connected to a first power device, which is fixed on the support rod, and the power device is used to provide power for the inoculation needle to move up and down.

[0012] Furthermore, the inoculation ball is connected to a second power device, the second power device is fixed on the support rod, and the second power device is used to provide power for the inoculation ball to move up and down.

[0013] Furthermore, the leaking piece is connected to a third power device, the third power device is fixed on the top of the inoculation hole, and the third power device is used to provide power for the leaking piece to rotate left and right.

[0014] Furthermore, the top end of the inoculation needle is connected to an injection valve, and the injection valve is connected to a liquid strain storage tank.

[0015] Furthermore, the liquid strain storage tank is provided with a sterile filtration vent and a stirring device inside.

[0016] Furthermore, the liquid strain storage tank is connected to the sterile water storage tank.

[0017] The inoculation method of the automatic inoculation device for the golden ear liquid bacteria of the present invention comprises the following steps:

[0018] S1. Place the bacteria bag on the conveyor belt, and the conveyor belt transfers the bacteria bag to the bottom of the inoculation needle and the inoculation ball;

[0019] S2. The inoculation needle and the inoculation ball descend synchronously, the inoculation needle pierces the plastic film of the bacteria bag, the needle tip and the inoculation hole enter the plastic film of the bacteria bag, and the inoculation ball presses a small pit on the bacteria bag;

[0020] S3. The inoculation ball rises to its original position, and the leak sheet rotates to open the plastic film of the bacteria bag located at the small pit, so that the plastic film and the small pit form an inoculation space;

[0021] S4. The Tremella aurantialba liquid spawn flows into the interior of the inoculation needle, and under the guiding action of the guiding inclined plane, it flows into the inoculation space, so that the Tremella aurantialba liquid spawn is inoculated onto the surface of the substrate of the mushroom bag.

[0022] S5. The leakage plate rotates to the original position, and the inoculation needle rises to the original position.

[0023] Furthermore, the inoculation method further includes the following steps:

[0024] S6. The conveyor belt conveys another mushroom bag to the lower part of the inoculation needle and the inoculation ball, and continues to execute steps S2 - S5.

[0025] Furthermore, in S4, the Tremella aurantialba liquid spawn quantitatively flows into the interior of the inoculation needle from the liquid spawn storage tank through the sampling valve.

[0026] The beneficial effects of the present invention are as follows:

[0027] The present invention is provided with an inoculation needle, an inoculation ball and a conveyor belt. The conveyor belt is used to place the mushroom bag. The mushroom bag can be conveyed by the conveyor belt to the lower part of the inoculation needle and the inoculation ball, and is automatically inoculated under the cooperation of the inoculation needle and the inoculation ball. Thus, a completely different inventive concept is adopted, and the injection of the Tremella aurantialba liquid spawn can be realized in batches and automatically. Description of the Drawings

[0028] In order to more clearly illustrate the technical solutions in the embodiments of the present invention, the following will briefly introduce the drawings required for the description of the embodiments. Obviously, the drawings in the following description are only the embodiments of the present invention. For those of ordinary skill in the art, without creative efforts, other drawings can also be obtained based on these drawings.

[0029] Figure 1 It is a schematic structural diagram of the first state of the automatic inoculation device for Tremella aurantialba liquid spawn in Embodiment 1.

[0030] Figure 2 It is a schematic structural diagram of the second state of the automatic inoculation device for Tremella aurantialba liquid spawn in Embodiment 1.

[0031] Figure 3 It is a schematic structural diagram of the third state of the automatic inoculation device for Tremella aurantialba liquid spawn in Embodiment 1.

[0032] Figure 4 It is a schematic structural diagram of the fourth state of the automatic inoculation device for Tremella aurantialba liquid spawn in Embodiment 1.

[0033] Figure 5 It is a schematic structural diagram of the fourth state of the automatic inoculation device for Tremella aurantialba liquid spawn in Embodiment 1.

[0034] Figure 6Schematic structural diagram of the first state of the Tremella aurantialba liquid spawn automatic inoculation device in Embodiment 2.

[0035] The serial numbers in the figure and the corresponding features are as follows:

[0036] 1 - inoculation needle; 2 - inoculation ball; 3 - conveyor belt; 4 - support rod; 5 - leakage plate; 6 - fungus bag; 7 - liquid spawn storage tank;

[0037] 11 - inoculation hole; 12 - diversion inclined plane; 13 - first power device; 14 - sampling valve; 21 - second power device; 51 - third power device; 71 - ventilation port; 72 - stirring device. Specific implementation mode

[0038] Next, the technical solutions in the embodiments of the present invention will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present invention.

[0039] Embodiment 1

[0040] See Figures 1 - 5 As shown, an automatic inoculation device for Tremella aurantialba liquid spawn of the present invention includes an inoculation needle 1, an inoculation ball 2, and a conveyor belt 3.

[0041] The inoculation needle 1 is connected to a support rod 4 in a liftable manner. The inoculation needle 1 can be filled with Tremella aurantialba liquid spawn inside. An inoculation hole 11 is opened on the side near the needle tip end of the inoculation needle 1. Specifically, an inoculation hole with a diameter of 4 - 10 mm can be opened on the side at a distance of 0.8 - 1.2 cm from the end of the needle tube of the inoculation needle for the outflow of Tremella aurantialba liquid spawn; a leakage plate 5 is rotatably connected to the top of the inoculation hole 11, and the leakage plate 5 can close the inoculation hole 11; a diversion inclined plane 12 is provided inside the inoculation needle 1, and the bottom end of the diversion inclined plane 12 abuts and is fixed to the bottom end of the inoculation hole; specifically, the diversion inclined plane has an inclined section for guiding the flow direction of Tremella aurantialba liquid spawn and reducing residue.

[0042] The inoculation ball 2 is arranged adjacent to the inoculation needle and can be connected to the support rod in a liftable manner;

[0043] The conveyor belt 3 is used to place the fungus bag 6, and the fungus bag 6 can be conveyed by the conveyor belt 3 to the lower part of the inoculation needle 1 and the inoculation ball 2 and is automatically inoculated under the cooperation of the inoculation needle 1 and the inoculation ball 2.

[0044] Among them, as a specific implementation mode, the inoculation needle 1 is connected to a first power device 13, and the first power device 13 is fixed on the support rod 4. The power device is used to provide the power for the up - and - down lifting of the inoculation needle.

[0045] As a specific implementation, the inoculation ball 2 is connected to a second power device 21, and the second power device 21 is fixed on the support rod 4. The second power device is used to provide power for the inoculation ball to move up and down.

[0046] As a specific implementation, the leaking piece 5 is connected to a third power device 51, and the third power device 51 is fixed on the top of the inoculation hole 11, and the third power device is used to provide power for the leaking piece to rotate left and right.

[0047] The first power device, the second power device and the third power device include but are not limited to, for example, motors or cylinders that can be used in the prior art. Specifically, the first power device uses a first motor or a first cylinder, the second power device uses a second motor or a second cylinder, and the third power device uses a third motor. It should be noted that the third motor can be a micro motor, and there are currently micro motors with diameters ranging from a few millimeters to more than ten millimeters on the market. It is currently a mature technology to achieve controllable lifting through motor or cylinder drive and control rotation through motor drive.

[0048] As a specific embodiment, the top of the inoculation needle 1 is connected to the injection valve 14, and the injection valve 14 is connected to the liquid bacterial storage tank 7. The injection valve 14 can adopt the quantitative control injection valve in the prior art for accurate quantification. The valve core surface of the injection valve can be further coated with an antibacterial coating to reduce bacterial liquid residue.

[0049] As a specific implementation, the liquid bacterial strain storage tank 7 is provided with a sterile filtration vent 71 and a stirring device 72 inside to prevent the bacterial strain from settling.

[0050] As a specific implementation, the liquid strain storage tank is connected to a sterile water storage tank (not shown) for mixing the Tremella liquid strain and sterile water in proportion for dilution when necessary to achieve a desired inoculation concentration.

[0051] The inoculation method of the Tremella fuciformis liquid strain automatic inoculation device of the present invention comprises the following steps:

[0052] S1. Place the bacteria bag 6 on the conveyor belt 3, and the conveyor belt 3 transfers the bacteria bag 6 to the bottom of the inoculation needle 1 and the inoculation ball 2;

[0053] S2. The inoculation needle 1 and the inoculation ball 2 are synchronously lowered, the inoculation needle 1 pierces the plastic film of the bacteria bag 6, the needle head and the inoculation hole 11 enter the plastic film of the bacteria bag, and the inoculation ball 2 presses a small pit 61 on the bacteria bag 6;

[0054] S3. The inoculation ball 2 rises to its original position, and the leak sheet 5 rotates to open the plastic film of the bacteria bag located at the small pit, so that the plastic film and the small pit form an inoculation space;

[0055] S4. The golden ear liquid bacteria flow from the inside of the inoculation needle 1 and flow into the inoculation space under the guidance of the guide slope 12, so that the golden ear liquid bacteria are inoculated onto the surface of the bacteria bag matrix;

[0056] S5. The leak sheet 5 rotates to its original position, and the inoculation needle 2 rises to its original position;

[0057] S6. The conveyor belt transfers another bacterial bag to the bottom of the inoculation needle and the inoculation ball, and steps S2-S5 are continued.

[0058] In this way, under the action of the conveyor belt, each fungus bag is conveyed one by one to the bottom of the inoculation needle and the inoculation ball, and then automatically inoculated with the cooperation of the inoculation needle and the inoculation ball. Therefore, the present invention adopts a completely different inventive concept and realizes the batch and automated injection of golden ear liquid fungus.

[0059] Can be combined with Figures 1 - 5 As shown, Figure 1 Corresponding to the first state of S1; Figure 2 Corresponding to the second state of S2, Figure 3 and Figure 4 Corresponding to the third and fourth states of S3; Figure 5 Corresponding to the fifth state of S4, Figure 5 The arrows in the figure indicate the flow direction of the Tricholoma auricularia liquid culture.

[0060] As a specific implementation, in S4, the golden ear liquid bacteria are quantitatively flowed from the liquid bacteria storage tank into the inoculation needle through the injection valve to achieve accurate quantitative inoculation control of each bacteria bag. In this way, the flow direction of the complete golden ear liquid bacteria is: liquid bacteria storage tank → injection valve → inoculation needle → inoculation space.

[0061] In the above working process, when the leak sheet is not in working state, the leak sheet and the inoculation hole form a closed state, realizing the closure of the inoculation hole, which can effectively prevent miscellaneous bacteria from invading the inoculation needle; when in working state, the leak sheet props up the plastic film to form an inoculation channel; the leak sheet can separate the surface covering on the bacterial bag, that is, the plastic film, from the matrix to form an inoculation space; the inoculation space is conducive to the accurate inoculation of the golden ear liquid strain to the matrix surface of the culture medium, improving the inoculation efficiency and uniformity. In addition, the diversion of the diversion slope makes the golden ear liquid strain be sprayed directionally to the inoculation space to complete the inoculation.

[0062] In summary, the present invention realizes the full-automatic, high-precision, and low-pollution inoculation of Tremella fuciformis liquid strains, significantly improves the inoculation efficiency and strain survival rate, and is suitable for factory-based Tremella fuciformis cultivation.

[0063] Example 2

[0064] Referring to Example 1, the difference from Example 1 is thatFigure 6 As shown in Figure 6 , this embodiment has 2 sets of combinations of inoculation balls and inoculation needles at the same time, which can achieve inoculating 2 places on the same spawn bag at one time. There is still one conveyor belt for conveying the spawn bags.

[0065] In summary, compared with the prior art such as CN118542191A, on the one hand, the present invention adopts the structure of the inoculation needle with a lateral opening and a diversion slope, and controls the flow rate of the bacterial liquid through the opening and closing of the leakage plate to ensure that the bacterial species accurately drip to the target position of the spawn bag. On the other hand, the present invention adopts the technical means of automatic inoculation with the cooperation of the inoculation needle and the inoculation ball. Among them, the inoculation ball pre-compacts the culture medium to form an inoculation pit, and then the inoculation needle injects bacteria laterally. A single lifting action completes the entire process of positioning and injecting bacteria. Coupled with the automatic transmission of the conveyor belt, it can realize the batch and automatic injection of Tremella aurantialba bacterial liquid. It adopts an inventive concept completely different from the prior art, and the present invention has higher adaptability for batch production. The present invention continuously conveys the spawn bags through the conveyor belt and cooperates with the liftable inoculation assembly (inoculation needle and inoculation ball) to realize assembly line operation, which is more suitable for industrial production scenarios.

[0066] The series of detailed descriptions listed above are only specific descriptions of the feasible embodiments of the present invention, and they are not intended to limit the protection scope of the present invention. Any equivalent embodiments or changes made without departing from the technical spirit of the present invention should be included in the protection scope of the present invention.

Claims

1. An automatic inoculation device for Tremella aurantialba liquid spawn, characterized in that, include: An inoculation needle is connected to a rod in a liftable manner. A liquid fungus of Tremella fuciformis can be loaded inside the inoculation needle. An inoculation hole is provided on the side near the end of the needle head of the inoculation needle. A leak sheet can be rotatably connected to the top of the inoculation hole, and the leak sheet can close the inoculation hole. A guide slope is provided inside the inoculation needle, and the bottom end of the guide slope is fixed against the bottom end of the inoculation hole. An inoculation ball, which is arranged adjacent to the inoculation needle and is liftably connected to the support rod; The conveyor belt is used for placing the fungus bag, which can be conveyed by the conveyor belt to the bottom of the inoculation needle and the inoculation ball, and automatically inoculated with the cooperation of the inoculation needle and the inoculation ball.

2. The Tremella aurantialba liquid spawn automatic inoculation device according to claim 1, wherein The inoculation needle is connected to a first power device, which is fixed on the support rod and is used to provide power for the inoculation needle to move up and down.

3. The Tremella aurantialba liquid spawn automatic inoculation device according to claim 1, characterized in that, The inoculation ball is connected to a second power device, which is fixed on the support rod and is used to provide power for the inoculation ball to move up and down.

4. The Tremella aurantialba liquid spawn automatic inoculation device according to claim 1, characterized in that, The leaking piece is connected with a third power device, and the third power device is fixed on the top of the inoculation hole, and the third power device is used to provide power for the leaking piece to rotate left and right.

5. The Tremella aurantialba liquid spawn automatic inoculation device according to claim 1, characterized in that, The top end of the inoculation needle is connected to an injection valve, and the injection valve is connected to a liquid bacteria storage tank.

6. The Tremella aurantialba liquid spawn automatic inoculation device according to claim 5, characterized in that, The liquid strain storage tank is provided with a sterile filtering vent and a stirring device is arranged inside.

7. The Tremella aurantialba liquid spawn automatic inoculation device according to claim 5, characterized in that The liquid strain storage tank is communicated with the sterile water storage tank.

8. An inoculation method of the Tremella fuciformis liquid culture automatic inoculation device according to any one of claims 1 to 7, comprising the following steps: S1. Place the bacteria bag on the conveyor belt, and the conveyor belt transfers the bacteria bag to the bottom of the inoculation needle and the inoculation ball; S2. The inoculation needle and the inoculation ball descend synchronously, the inoculation needle pierces the plastic film of the bacteria bag, the needle tip and the inoculation hole enter the plastic film of the bacteria bag, and the inoculation ball presses a small pit on the bacteria bag; S3. The inoculation ball rises to its original position, and the leak sheet rotates to open the plastic film of the bacteria bag located at the small pit, so that the plastic film and the small pit form an inoculation space; S4. The golden ear liquid bacteria flow from the inside of the inoculation needle and flow into the inoculation space under the diversion effect of the diversion slope, so that the golden ear liquid bacteria are inoculated onto the surface of the bacteria bag matrix; S5. The leak sheet rotates to its original position and the inoculation needle rises to its original position.

9. The inoculation method according to claim 8, wherein, The following steps are also included: S6. The conveyor belt transfers another bacterial bag to the bottom of the inoculation needle and the inoculation ball, and steps S2-S5 are continued.

10. The inoculation method according to claim 8, wherein In S4, the Tremella fuciformis liquid culture flows quantitatively from the liquid culture storage tank into the interior of the inoculation needle through the injection valve.

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