Induction device for rapid propagation of tissue culture Chinese herbaceous peony flowers

Abstract

The invention provides an induction device for rapid propagation of tissue culture Chinese herbaceous peony flowers, belongs to the technical field of flower tissue culture, and solves the technical problems of high pollution rate, low induction efficiency and the like of the existing Chinese herbaceous peony tissue culture. Comprising an air filter, a support mechanism, a temperature controller, an atomization humidifier and a gas storage tank, a shading mechanism is arranged on the support mechanism, a movable plate mechanism is hinged to the shading mechanism, a rotating shaft mechanism is rotationally arranged on the support mechanism, a gear motor is arranged on the support mechanism, and a rotating mechanism is arranged on the rotating shaft mechanism; the rotating mechanism is provided with a plurality of drawing mechanisms, and each drawing mechanism is provided with two fixing mechanisms. The device can disinfect the interior, ensure the sterile environment, drive the culture bottle to move, avoid metabolite enrichment, shorten the induction period, improve the induction efficiency, control the environment, shorten the tissue culture period, realize rapid propagation through light supplement and dynamic adjustment, fix the culture bottle, rapidly take out the culture bottle failed in induction, and reduce pollution.

Description

Technical Field

[0001] The present invention belongs to the technical field of flower tissue culture, and relates to a rapid propagation induction device for flowers, in particular to an induction device for rapid propagation of tissue-cultured peony flowers. Background Art

[0002] Peony has both medicinal and ornamental values. The global annual demand exceeds 200 million plants. The multiplication coefficient of rhizome division is low, and the sowing cycle is long, making it difficult to meet the demand for large-scale production. Tissue-culture rapid propagation can be used for large-scale breeding. Tissue-culture rapid propagation refers to the process of culturing isolated flower organs, tissues, cells, and protoplasts on an artificially prepared culture medium under sterile conditions, with appropriate culture conditions, to grow into complete plants. However, traditional open operations lead to a high pollution rate, and it is difficult to control environmental factors such as light, air, temperature, and humidity. Moreover, static culture results in the deposition of metabolites and a decrease in oxygen permeability, resulting in a long breeding cycle and low efficiency of tissue-culture rapid propagation.

[0003] Therefore, we propose an induction device for rapid propagation of tissue-cultured peony flowers, which can disinfect the interior to ensure a sterile environment inside, drive the culture bottle to rotate to avoid metabolite enrichment, promote the dedifferentiation of explants, shorten the callus induction cycle, enhance oxygen penetration, improve the induction and differentiation efficiency, form a photosynthesis-like environment with constant temperature, high humidity, and rich CO2 to ensure rapid induction of tissue culture, can provide supplementary light inside, dynamically adjust the light intensity, light time, and light color to ensure the rapid propagation of peony flowers, can keep the culture bottle stable, and quickly remove the contaminated culture bottle in case of induction failure, thereby reducing internal pollution and ensuring a clean culture environment. Summary of the Invention

[0004] The purpose of the present invention is to address the above problems existing in the prior art and propose an induction device for rapid propagation of tissue-cultured peony flowers. The technical problem to be solved by this invention is: how to achieve sterile and efficient induction tissue culture of peony flowers to meet the demand for rapid propagation.

[0005] The purpose of the present invention can be achieved by the following technical solutions: An induction device for rapid propagation of tissue-cultured peony flowers, comprising an air filter, a support mechanism, a temperature controller, an atomizing humidifier and a gas storage tank arranged in sequence from left to right. A light-shielding mechanism is provided on the support mechanism, a movable plate mechanism is hinged on the light-shielding mechanism, a rotating shaft mechanism is rotatably provided on the support mechanism, a deceleration motor is provided on the support mechanism, and the output shaft of the deceleration motor is in transmission connection with the rotating shaft mechanism. A rotating mechanism is provided on the rotating shaft mechanism, and the rotating mechanism is located inside the light-shielding mechanism. A plurality of pull-out groups are detachably provided on the rotating mechanism. Each pull-out group is composed of a plurality of pull-out mechanisms. Two fixing mechanisms are symmetrically arranged front and back on each pull-out mechanism. The air inlet of the temperature controller is connected to the gas storage tank and the atomizing humidifier respectively through a bifurcated pipeline. The air outlet of the temperature controller is connected to the rotating shaft mechanism through a pipeline. The light-shielding mechanism is connected to the air filter through a pipeline. A plurality of humidity sensors, a plurality of CO2 concentration sensors and a plurality of light sensors are arranged inside the light-shielding mechanism.

[0006] The working principle of the present invention is: before induction, the light-shielding mechanism is started and ultraviolet disinfection is carried out inside to ensure a sterile induction environment; The movable plate mechanism is opened, and the staff sequentially fixes the culture bottles containing peony tissues on a plurality of fixing mechanisms. After completion, the movable plate mechanism and the light-shielding mechanism cooperate to form a closed lightless environment. Subsequently, the output shaft of the deceleration motor drives the rotating shaft mechanism to rotate, the rotating shaft mechanism drives the rotating mechanism to move, and the rotating mechanism drives a plurality of fixing mechanisms to move and the culture bottles thereon to perform circular motion, avoiding local enrichment of metabolites caused by static culture medium, improving the uniformity of proliferation. At the same time, the low-speed rotation of the mounting frame promotes the dedifferentiation of explants, shortens the callus induction period, and enhances oxygen penetration, avoiding cell necrosis caused by anaerobic metabolism. At the same time, a plurality of humidity sensors, a plurality of CO2 concentration sensors and a plurality of light sensors regularly detect the humidity, gas concentration and light in the light-shielding mechanism. The gas storage tank quantitatively transports CO2 according to the gas concentration in the light-shielding mechanism to maintain an environment rich in CO2 in the light-shielding mechanism. The atomizing humidifier quantitatively transports atomized water vapor according to the humidity in the light-shielding mechanism to ensure a high-humidity environment in the light-shielding mechanism. Both CO2 and atomized water vapor are transported into the light-shielding mechanism through the rotating shaft mechanism, and the temperature controller bifurcated pipeline integrates the gas storage tank and the atomizing humidifier to form a constant-temperature, high-humidity and CO2-rich photosynthetic-like environment, promoting the improvement of the carbon metabolism efficiency of callus. The air in the light-shielding mechanism is transported into the air filter through a pipeline, and the air filter continuously purifies the inlet air. Combined with the dynamic air flow driven by the rotation of the rotating mechanism, the internal gas flow is ensured and pollution is reduced; When switching to light culture, the shading mechanism supplements the interior with light and adjusts the light intensity, time and color according to the induction of the peony tissue. After the culture is completed, the culture bottle on the fixing mechanism at the corresponding position is taken out through the pull-out mechanism, which is quick and convenient. After detecting that the induction failure of the internal culture bottle occurs, the contaminated culture bottle is quickly taken out through the cooperation of the pull-out mechanism and the fixing mechanism to avoid direct touch, thereby reducing internal contamination. After the tissue culture is completed, the staff pulls the pull-out mechanism to take out the culture bottle fixed on the fixing mechanism, which is convenient for the staff to take out the peony plants bred by tissue culture.

[0007] The support mechanism comprises a frame, a mounting seat is arranged on the side of the frame, and the reduction motor is fixed on the mounting seat.

[0008] With the above structure, the frame is used to support the rotating mechanism to avoid collision.

[0009] The rotating mechanism includes two mounting frames that are symmetrically arranged on the left and right, and the two mounting frames are respectively arranged at the two ends of the rotating shaft mechanism. Six balancing frames that are evenly distributed in a circle are rotatably arranged on the mounting frames. The balancing frames are in a triangular structure, and the tops of the balancing frames are arranged on the mounting frames. The positions of the balancing frames of the two mounting frames correspond to each other. A placement plate is detachably provided between the two balancing frames at the corresponding positions of the two mounting frames, and the two ends of the placement plate are respectively arranged on both sides of the bottom of the balancing frames at the corresponding positions.

[0010] With the above structure, when dark culture and light culture are carried out, the output shaft of the reduction motor drives the rotating shaft mechanism to move, and the rotating shaft mechanism drives the mounting frame to move. When the mounting frame moves, the balance frame rotates on the mounting frame, and at the same time drives the placement plate at the corresponding position to move. During the movement, the triangular structure of the balance frame is relied on to keep the placement plate level to ensure stable movement.

[0011] The shading mechanism includes two symmetrically arranged fixing plates, which are respectively fixed on both sides of the inner upper end of the frame, and a light shield is fixed between the two fixing plates. A plurality of circumferentially evenly distributed ultraviolet disinfection lamps and a plurality of circumferentially evenly distributed multi-color fill lights and a plurality of cameras are arranged inside the light shield. The plurality of multi-color fill lights and the plurality of ultraviolet disinfection lamps are staggered, and an observation port is opened on the light shield.

[0012] With the above structure, the fixed plate is used for fixing, the light shield is used for isolating light, and the movable plate mechanism and the door panel mechanism are cooperated to form a closed environment, a number of ultraviolet disinfection lamps are used to disinfect the inside of the light shield to ensure the internal sterile environment, a number of cameras monitor the induced growth of the internal peony, a number of multi-color fill lights are used to provide light, and according to the induced culture of the detected peony tissue, the light intensity, light time and light color are adjusted to reduce the induction time, realize the rapid propagation of peony flowers through tissue culture, and facilitate the adjustment of the light environment inside the light shield, thereby dynamically adjusting the culture environment.

[0013] The movable plate mechanism includes a movable plate, on which an annular sealing gasket is provided. The position and inner ring size of the annular sealing gasket correspond to the observation port. A number of equally spaced movable hinges are provided at the upper end of the movable plate, and the movable hinges are all fixed on the light-shielding cover. Both sides of the rear end of the movable plate are hinged with electric push rods, and the ends of the electric push rods are all hinged inside the light-shielding cover.

[0014] With the above structure, after placing the culture bottle, the telescopic ends of the two electric push rods extend and push the movable plate. The movable plate is opened through a number of movable hinges, exposing the observation port, which is convenient for placing the culture bottle through the observation port. The position and inner size of the annular sealing gasket correspond to the observation port, and the lower end surface of the annular sealing gasket is higher than the lower end surface of the observation port, avoiding the outflow of internal water vapor and causing pollution.

[0015] The drawing mechanism includes a fixed seat and a drawing plate. Each fixed seat is detachably arranged on the placing plate at the corresponding position. On both sides inside the fixed seat, a first chute is opened. At both ends of the upper end of the fixed seat, a second clamping groove is opened. Inside the second clamping groove, a clamping block is rotatably arranged. On both sides of the drawing plate, a first slider is fixed. The two first sliders are symmetrically arranged. The position and size of the first slider correspond to the first chute, and the first sliders are all slidably arranged in the first chute at the corresponding position. At the end of the drawing plate, a drawing handle is provided. On both sides of the drawing handle, a first clamping groove is opened. The position of the first clamping groove corresponds to the second clamping groove, and the shape and size of the first clamping groove correspond to the clamping block.

[0016] With the above structure, select a culture bottle with a suitable specification according to the type of peony flower, and select a drawing plate with a suitable specification. Before placing the culture bottle, install the drawing plate on the fixed seat, make the two first sliders slidably installed in the first chute at the corresponding position, and push the drawing plate against the fixed seat. Then, snap the two clamping blocks into the first clamping groove and the second clamping groove at the corresponding position, thereby fixing the drawing plate on the fixed seat; After the cultivation is completed, the staff push the clamping block to make the clamping block away from the first clamping groove at the corresponding position, and then pull the drawing handle to make the drawing plate leave the fixed seat, thereby driving the fixing mechanism out of the light-shielding cover.

[0017] The fixing mechanism includes an electric base, a limiting ring and a second slider. The limiting ring is detachably arranged on the outer upper end of the electric base. The electric base is detachably arranged on the drawing plate. A number of second chutes evenly distributed in a circle are opened on the electric base. Inside the second chutes, second sliders are all slidably arranged. A return spring is fixed between the second sliders and the limiting ring. At the end of the second slider, a fixing rod is provided. The fixing rod is perpendicular to the corresponding second slider. At the end of the fixing rod, an arc-shaped guiding block is provided. A number of fixing rods cooperate with each other to form a fixing groove.

[0018] With the above structure, select a culture bottle of appropriate specifications according to the type of peony flowers, and select an electric base of appropriate specifications. When placing the culture bottle, place the culture bottle on a number of arc-shaped guide blocks, and push the number of arc-shaped guide blocks. The number of arc-shaped guide blocks drives the corresponding slider two to slide in the chute two. The number of sliders two pushes the corresponding return spring to move, so that the fixing grooves formed by the cooperation of the number of fixing rods fit the culture bottle, and through the reaction of the number of return springs, the number of fixing rods abut against the culture bottle to fix the culture bottle, ensuring that the culture bottle remains stable during rotational movement. The electric base is internally provided with a storage battery for power supply. The electric base drives a number of fixing rods and the culture bottle to rotate slowly, and cooperates with the rotating mechanism to avoid local enrichment of metabolites, enhance oxygen penetration, and improve the efficiency of tissue culture and rapid propagation.

[0019] The rotation shaft mechanism includes a hollow rotating shaft. The hollow rotating shaft is rotatably arranged at the upper end of the frame. A number of ventilation holes two are arranged on the hollow rotating shaft in a circumferentially uniform distribution. Inside the hollow rotating shaft, there is a structure. Outside the hollow rotating shaft, there is a delivery pipe. A number of ventilation holes one are arranged on the delivery pipe in a circumferentially uniform distribution. The ventilation holes one and the ventilation holes two are staggered and communicate with each other.

[0020] With the above structure, atomized water vapor and CO2 gas enter the hollow rotating shaft, enter the delivery pipe through a number of ventilation holes two, and are evenly delivered into the light-shielding cover through a number of ventilation holes one on the delivery pipe, ensuring a high-humidity and CO2-rich environment inside.

[0021] Compared with the prior art, the induction device for rapid propagation of tissue-cultured peony flowers has the following advantages: 1. Through the cooperation of the movable plate mechanism and the light-shielding mechanism, a closed lightless environment is formed, and the inside is disinfected through the light-shielding mechanism to ensure a sterile environment inside.

[0022] 2. The output shaft of the reduction motor drives the rotating mechanism to move, thereby driving a number of fixing mechanisms to move and the culture bottles thereon to perform circular motion, avoiding metabolite enrichment, improving the uniformity of proliferation, promoting the dedifferentiation of explants through slow rotation, shortening the callus induction period, enhancing oxygen penetration, and ensuring the induction and differentiation efficiency.

[0023] 3. The light-shielding mechanism regularly detects the internal environment. The gas storage tank quantitatively delivers CO2 according to the detection data, and the atomizing humidifier quantitatively delivers atomized water vapor according to the detection data. In cooperation with the temperature controller, the gas storage tank and the atomizing humidifier are integrated to form a constant-temperature, high-humidity and CO2-rich photosynthetic-like environment to ensure rapid induction of tissue culture.

[0024] 4. The light-shielding mechanism provides supplementary light inside, and in cooperation with the rotating mechanism, dynamically adjusts the light intensity, light time and light color to ensure the rapid breeding of peony flowers.

[0025] 5. Fix the culture flask through the fixing mechanism, so that when the culture flask moves with the rotating mechanism, it remains stable and cooperates with the pulling mechanism. When the induction fails, the contaminated culture flask can be quickly taken out through the pulling mechanism, thereby reducing internal contamination and ensuring a clean culture environment. Brief Description of the Drawings

[0026] Figure 1 is a schematic structural diagram of the present invention.

[0027] Figure 2 is a schematic structural diagram of some components in the present invention.

[0028] Figure 3 is a schematic structural diagram of the bracket mechanism and the rotating shaft mechanism in the present invention.

[0029] Figure 4 is a schematic structural diagram of the rotating mechanism in the present invention.

[0030] Figure 5 is a three-dimensional structural diagram of the light-shielding mechanism in the present invention.

[0031] Figure 6 is a partially sectional view of the light-shielding mechanism in the present invention.

[0032] Figure 7 is a schematic structural diagram of the movable plate mechanism in the present invention.

[0033] Figure 8 is a schematic structural diagram of the pulling mechanism and the fixing mechanism in the present invention.

[0034] Figure 9 is a schematic structural diagram of the pulling mechanism in the present invention.

[0035] Figure 10 is a schematic structural diagram of the fixing mechanism in the present invention.

[0036] Figure 11 is a three-dimensional structural diagram of the rotating shaft mechanism in the present invention.

[0037] Figure 12 is a partially sectional view of the rotating shaft mechanism in the present invention.

[0038] In the figure, 1 is an air filter; 2 is a bracket mechanism; 3 is a movable plate mechanism; 4 is a temperature controller; 5 is an atomizing humidifier; 6 is a gas storage tank; 7 is a light-shielding mechanism; 8 is a rotating shaft mechanism; 9 is a frame; 10 is a placement plate; 11 is a mounting bracket; 12 is a balance bracket; 13 is a mounting seat; 14 is a reduction motor; 15 is a light-shielding cover; 16 is an observation port; 17 is an ultraviolet disinfection lamp; 18 is a multicolor supplementary light; 19 is a fixing plate; 20 is an electric push rod; 21 is an annular gasket; 22 is a movable plate; 23 is a movable hinge; 24 is a pulling mechanism; 25 is a fixing mechanism; 26 is a pulling plate; 27 is a fixing seat; 28 is a first chute; 29 is a clamping block; 30 is a first slider; 31 is a first clamping groove; 32 is a pulling handle; 33 is a fixing rod; 34 is an arc-shaped guiding block; 35 is an electric base; 36 is a return spring; 37 is a second chute; 38 is a second slider; 39 is a first ventilation hole; 40 is a rotating shaft; 41 is a limiting ring; 42 is a delivery pipe; 43 is a second ventilation hole; 44 is a rotating mechanism. Detailed implementation manners

[0039] The following are specific embodiments of the present invention and in combination with the accompanying drawings, the technical solutions of the present invention are further described, but the present invention is not limited to these embodiments.

[0040] As Figures 1-12 shown, the induction device for rapid propagation of tissue-cultured peony flowers includes an air filter 1, a bracket mechanism 2, a temperature controller 4, an atomizing humidifier 5, and a gas storage tank 6 arranged in sequence from left to right. A light-shielding mechanism 7 is provided on the bracket mechanism 2. A movable plate mechanism 3 is hinged on the light-shielding mechanism 7. A rotating shaft mechanism 8 is rotatably provided on the bracket mechanism 2. A reduction motor 14 is provided on the bracket mechanism 2. The output shaft of the reduction motor 14 is in transmission connection with the rotating shaft mechanism 8. A rotating mechanism 44 is provided on the rotating shaft mechanism 8, and the rotating mechanism 44 is located inside the light-shielding mechanism 7. A plurality of sets of pulling groups are detachably provided on the rotating mechanism 44. Each set of pulling groups is composed of a plurality of pulling mechanisms 24. Two fixing mechanisms 25 are symmetrically arranged front and back on each pulling mechanism 24. The air inlet of the temperature controller 4 is connected to the gas storage tank 6 and the atomizing humidifier 5 respectively through a bifurcated pipeline. The air outlet of the temperature controller 4 is connected to the rotating shaft mechanism 8 through a pipeline. The light-shielding mechanism 7 is connected to the air filter 1 through a pipeline. A plurality of humidity sensors, a plurality of CO2 concentration sensors, and a plurality of light sensors are provided inside the light-shielding mechanism 7.

[0041] In this embodiment, culture bottles, fixing mechanisms 25, and pulling mechanisms 24 of appropriate specifications are selected according to the types of peony flowers to meet the tissue culture induction requirements of different types of peony flowers. The staff sequentially fix the culture bottles containing peony tissues on a number of fixing mechanisms 25, and at the same time perform dynamic adjustment to conduct the peony tissue culture induction experiment, obtaining the optimal data of light time, light color, light intensity, temperature, humidity, and CO2 gas concentration, and conducting the tissue culture rapid propagation induction of peony flowers according to the obtained data; Before induction, the light-shielding mechanism 7 is activated, and ultraviolet disinfection is carried out inside to ensure a sterile induction environment; The movable plate mechanism 3 is opened. The staff sequentially fix the culture bottles containing peony tissues on a number of fixing mechanisms 25. After completion, the movable plate mechanism 3 and the light-shielding mechanism 7 cooperate to form a closed lightless environment. Subsequently, the output shaft of the reduction motor 14 drives the rotating shaft mechanism 8 to rotate. The rotating shaft mechanism 8 drives the rotating mechanism 44 to move. The rotating mechanism 44 drives a number of fixing mechanisms 25 and the culture bottles thereon to perform circular motion, avoiding local enrichment of metabolites caused by the static culture medium, improving the proliferation uniformity. At the same time, the mounting frame rotates at a low speed to promote the dedifferentiation of explants, shorten the callus induction cycle, and enhance oxygen penetration, avoiding cell necrosis caused by anaerobic metabolism. At the same time, a number of humidity sensors, a number of CO2 concentration sensors, and a number of light sensors regularly detect the humidity, gas concentration, and light inside the light-shielding mechanism 7. The gas storage tank 6 quantitatively transports CO2 according to the gas concentration inside the light-shielding mechanism 7 to maintain a CO2-rich environment inside the light-shielding mechanism 7. The atomizing humidifier 5 quantitatively transports atomized water vapor according to the humidity inside the light-shielding mechanism 7 to ensure a high-humidity environment inside the light-shielding mechanism 7. Both CO2 and atomized water vapor are transported into the light-shielding mechanism 7 through the rotating shaft mechanism 8, and the temperature controller 4 bifurcates the pipeline to integrate the gas storage tank 6 and the atomizing humidifier 5 to form a constant-temperature, high-humidity, and CO2-rich photosynthetic-like environment, promoting the improvement of the carbon metabolism efficiency of callus. The air inside the light-shielding mechanism 7 is transported into the air filter 1 through the pipeline. The air filter 1 continuously purifies the incoming air, combined with the dynamic air flow driven by the rotation of the rotating mechanism 44, to ensure the internal gas flow and reduce pollution; When switching to light culture, the light-shielding mechanism 7 performs supplementary lighting inside and adjusts the light intensity, light time, and light color according to the induction situation of the peony tissues. After completion of the culture, the culture bottles on the fixing mechanisms 25 at the corresponding positions are taken out through the pulling mechanism 24 quickly and conveniently. And after detecting the induction failure of the internal culture bottles, through the cooperation of the pulling mechanism 24 and the fixing mechanism 25, the contaminated culture bottles are quickly taken out to avoid direct contact, thereby reducing internal pollution. After the tissue culture is completed, the staff pulls the pulling mechanism 24 to take out the culture bottles fixed on the fixing mechanism 25, which is convenient for the staff to take out the peony plants obtained by tissue culture and propagation.

[0042] The support mechanism 2 includes a frame 9 , a mounting seat 13 is provided on the side of the frame 9 , and a reduction motor 14 is fixed on the mounting seat 13 .

[0043] In this embodiment, the frame 9 is used to support the rotating mechanism 44 to avoid collision.

[0044] The rotating mechanism 44 includes two mounting frames 11 symmetrically arranged on the left and right, and the two mounting frames 11 are respectively arranged at the two ends of the rotating shaft mechanism 8. Six balancing frames 12 evenly distributed in a circle are rotatably arranged on the mounting frames 11. The balancing frames 12 are in a triangular structure, and the top of the balancing frames 12 is arranged on the mounting frames 11. The positions of the balancing frames 12 of the two mounting frames 11 correspond to each other. A placement plate 10 is detachably provided between the two balancing frames 12 at the corresponding positions of the two mounting frames 11, and the two ends of the placement plate 10 are respectively arranged on both sides of the bottom of the balancing frames 12 at the corresponding positions.

[0045] In this embodiment, when dark culture and light culture are carried out, the output shaft of the reduction motor 14 drives the rotating shaft mechanism 8 to move, and the rotating shaft mechanism 8 drives the mounting frame 11 to move. When the mounting frame 11 moves, the balance frame 12 rotates on the mounting frame 11, and at the same time drives the placement plate 10 at the corresponding position to move. During the movement, the triangular structure of the balance frame 12 is relied on to keep the placement plate 10 level to ensure stable movement.

[0046] The shading mechanism 7 includes two symmetrically arranged fixing plates 19, which are respectively fixed on both sides of the inner upper end of the frame 9, and a shading cover 15 is fixed between the two fixing plates 19. The shading cover 15 is provided with a plurality of circumferentially evenly distributed ultraviolet disinfection lamps 17 and a plurality of circumferentially evenly distributed multi-color fill lights 18 and a plurality of cameras. The plurality of multi-color fill lights 18 and the plurality of ultraviolet disinfection lamps 17 are staggeredly distributed, and an observation port 16 is provided on the shading cover 15.

[0047] In this embodiment, the fixed plate 19 is used for fixing, the light shield 15 is used for light isolation, and the movable plate mechanism 3 and the door panel mechanism form a closed environment. A number of ultraviolet disinfection lamps 17 are used for disinfecting the inside of the light shield 15 to ensure the internal sterile environment. A number of cameras monitor the induced growth of the internal peony. A number of multi-color fill lights 18 are used to provide lighting, and according to the induced culture of the detected peony tissue, the light intensity, lighting time and lighting color are adjusted to reduce the induction time, realize the rapid propagation of peony flowers through tissue culture, and facilitate the adjustment of the light environment inside the light shield 15, thereby dynamically adjusting the culture environment.

[0048] The movable plate mechanism 3 includes a movable plate 22. An annular gasket 21 is provided on the movable plate 22. The position and inner ring size of the annular gasket 21 correspond to the observation port 16. A number of equally spaced movable hinges 23 are provided at the upper end of the movable plate 22, and the number of movable hinges 23 are all fixed on the light shield 15. Both sides of the rear end of the movable plate 22 are hinged with electric push rods 20, and the ends of the electric push rods 20 are hinged inside the light shield 15.

[0049] In this embodiment, after placing the culture bottle, the telescopic ends of the two electric push rods 20 extend and push the movable plate 22. The movable plate is opened through a number of movable hinges 23, exposing the observation port 16, facilitating the placement of the culture bottle from the observation port 16. The position and inner ring size of the annular gasket 21 correspond to the observation port 16, and the lower end surface of the annular gasket 21 is higher than the lower end surface of the observation port 16, preventing internal water vapor from flowing out and causing pollution.

[0050] The pulling mechanism 24 includes a fixed seat 27 and a pulling plate 26. Each fixed seat 27 is detachably arranged on the placement plate 10 at the corresponding position. Both sides inside the fixed seat 27 are provided with a first chute 28. Both ends of the upper end of the fixed seat 27 are provided with a second card slot. A clamping block 29 is rotatably arranged inside the second card slot. Both sides of the pulling plate 26 are fixed with a first slider 30. The two first sliders 30 are symmetrically arranged. The position and size of the first slider 30 correspond to the first chute 28, and the first sliders 30 are all slidably arranged in the first chute 28 at the corresponding position. The end of the pulling plate 26 is provided with a pulling handle 32. Both sides of the pulling handle 32 are provided with a first card slot 31. The position of the first card slot 31 corresponds to the second card slot, and the shape and size of the first card slot 31 correspond to the clamping block 29.

[0051] In this embodiment, select a culture bottle with a suitable specification according to the type of peony flower, and select a pulling plate 26 with a suitable specification. Before placing the culture bottle, install the pulling plate 26 on the fixed seat 27, so that the two first sliders 30 are slidably installed in the first chute 28 at the corresponding position, and push the pulling plate 26 to abut against the fixed seat 27. Subsequently, buckle the two clamping blocks 29 inside the first card slot 31 and the second card slot at the corresponding position, thereby fixing the pulling plate 26 on the fixed seat 27; After the cultivation is completed, the staff pushes the clamping block 29 to make the clamping block 29 away from the first card slot 31 at the corresponding position, and then pulls the pulling handle 32 to make the pulling plate 26 leave the fixed seat 27, thereby driving the fixing mechanism 25 out of the light shield 15.

[0052] The fixing mechanism 25 includes an electric base 35, a limiting ring 41 and a second slider 38. The limiting ring 41 is detachably arranged at the upper end outside the electric base 35. The electric base 35 is detachably arranged on the drawer plate 26. A number of second chutes 37 evenly distributed in a circular pattern are formed in the electric base 35. Second sliders 38 are slidably arranged in the second chutes 37 respectively. Reset springs 36 are fixed between the second sliders 38 and the limiting ring 41. Fixing rods 33 are arranged at the ends of the second sliders 38. The fixing rods 33 are perpendicular to the corresponding second sliders 38. Arc-shaped guiding blocks 34 are arranged at the ends of the fixing rods 33. The fixing rods 33 cooperate with each other to form a fixing groove.

[0053] In this embodiment, a culture bottle with a suitable specification is selected according to the type of peony flower, and an electric base 35 with a suitable specification is selected. When placing the culture bottle, the culture bottle is placed on a number of arc-shaped guiding blocks 34, and the arc-shaped guiding blocks 34 are pushed. The arc-shaped guiding blocks 34 drive the corresponding second sliders 38 to slide in the second chutes 37. The second sliders 38 push the corresponding reset springs 36 to move, so that the fixing groove formed by the cooperation of the fixing rods 33 fits the culture bottle, and through the reaction of the reset springs 36, the fixing rods 33 abut against the culture bottle to fix the culture bottle, ensuring that the culture bottle remains stable during the rotational movement. A storage battery is built into the electric base 35 for power supply. The electric base 35 drives a number of fixing rods 33 and the culture bottle to rotate at a low speed and cooperate with the rotating mechanism 44 to avoid local enrichment of metabolites, enhance oxygen penetration, and improve the efficiency of tissue culture and rapid propagation.

[0054] The rotating shaft mechanism 8 includes a hollow rotating shaft 40. The hollow rotating shaft 40 is rotatably arranged at the upper end of the frame 9. A number of second ventilation holes 43 evenly distributed in a circular pattern are formed in the hollow rotating shaft 40. A conveying pipe 42 is arranged outside the hollow rotating shaft 40. A number of first ventilation holes 39 evenly distributed in a circular pattern are formed in the conveying pipe 42. The first ventilation holes 39 and the second ventilation holes 43 are staggered and communicated with each other.

[0055] In this embodiment, atomized water vapor and CO2 gas enter the hollow rotating shaft 40, enter the conveying pipe 42 through the second ventilation holes 43, and are evenly conveyed into the light-shielding cover 15 through the first ventilation holes 39 on the conveying pipe 42 to ensure a high-humidity and CO2-rich environment inside.

[0056] The working principle of the present invention: A culture bottle, a fixing mechanism 25 and a drawer mechanism 24 with suitable specifications are selected according to the type of peony flower to meet the tissue culture induction needs of different types of peony flowers. The staff fixes the culture bottles containing peony tissues on a number of fixing mechanisms 25 in turn and adjusts dynamically at the same time to conduct the peony tissue culture induction experiment, obtaining the optimal data of illumination time, illumination color, illumination intensity, temperature, humidity and CO2 gas concentration, and conducting the peony flower tissue culture and rapid propagation induction according to the obtained data. Before induction, several ultraviolet disinfection lamps 17 perform ultraviolet light disinfection on the interior to ensure a sterile induction environment. The movable plate mechanism 3 is opened, that is, the telescopic ends of the two electric push rods 20 extend and push the movable plate 22 to open the movable plate, exposing the observation port 16, facilitating the placement of the culture bottle through the observation port 16. The staff sequentially fix the culture bottles containing peony tissues on several fixing mechanisms 25. That is, when placing the culture bottle, place the culture bottle on several arc-shaped guide blocks 34 and push several arc-shaped guide blocks 34. Several arc-shaped guide blocks 34 drive the corresponding slider two 38 to slide in the chute two 37. Several slider two 38 push the corresponding return spring 36 to move, so that the fixing grooves formed by the cooperation of several fixing rods 33 fit the culture bottle, and through the reaction of several return springs 36, several fixing rods 33 abut against the culture bottle to fix the culture bottle, ensuring that the culture bottle remains stable during rotational movement. After completion, the movable plate mechanism 3 and the light-shielding mechanism 7 cooperate to form a closed lightless environment. Subsequently, the output shaft of the reduction motor 14 drives the rotating shaft mechanism 8 to rotate, that is, the output shaft of the reduction motor 14 drives the hollow rotating shaft 40 to rotate, and the hollow rotating shaft 40 drives the rotating mechanism 44 to move. The rotating mechanism 44 drives several fixing mechanisms 25 to move and the culture bottles thereon to perform circular motion. That is, the output shaft of the reduction motor 14 drives the rotating shaft mechanism 8 to move, and the rotating shaft mechanism 8 drives the mounting frame 11 to move. When the mounting frame 11 moves, the balance frame 12 rotates on the mounting frame 11, and at the same time drives the corresponding placement plate 10 to move. During the movement, relying on the triangular structure of the balance frame 12, the placement plate 10 is kept horizontal to ensure stable movement. The placement plate drives several fixing mechanisms 25 to move and the culture bottles thereon to perform circular motion, avoiding local enrichment of metabolites caused by the static culture medium, improving the proliferation uniformity. At the same time, the low-speed rotation of the mounting frame promotes the dedifferentiation of explants, shortens the callus induction cycle, and enhances oxygen penetration, avoiding cell necrosis caused by anaerobic metabolism. At the same time, the gas concentration, humidity and temperature in the light-shielding mechanism 7 are detected regularly. The gas storage tank 6 transports a certain amount of CO2 according to the gas concentration in the light-shielding mechanism 7 to maintain an environment rich in CO2 in the light-shielding mechanism 7. The atomizing humidifier 5 transports a certain amount of atomized water vapor according to the humidity in the light-shielding mechanism 7 to ensure a high-humidity environment in the light-shielding mechanism 7. Both CO2 and atomized water vapor are transported into the light-shielding mechanism 7 through the rotating shaft mechanism 8, that is, the atomized water vapor and CO2 gas enter the hollow rotating shaft 40, and enter the delivery pipe 42 through several vent holes two 43, and are evenly transported into the interior of the light-shielding cover 15 through several vent holes one 39 on the delivery pipe 42. And through the temperature controller 4, the forked pipeline integrates the gas storage tank 6 and the atomizing humidifier 5 to form a photosynthetic-like environment with constant temperature, high humidity and rich CO2, promoting the improvement of the carbon metabolism efficiency of callus. The air in the light-shielding mechanism 7 is transported into the air filter 1 through a pipeline. The air filter 1 continuously purifies the incoming air. Combined with the dynamic air flow driven by the rotation of the rotating mechanism 44, the internal gas flow is ensured and pollution is reduced; When switching to light culture, several cameras monitor the induced growth of internal peonies. Several multi-color supplementary lights 18 are used to provide light, and according to the detected induced culture conditions of the peony tissues, the light intensity, light time, and light color are adjusted to reduce the induction time, achieve the rapid propagation of tissue culture of peony flowers, facilitate the adjustment of the light environment inside the light-shielding cover 15, thereby dynamically adjusting the culture environment. After the culture is completed, the culture bottle on the fixing mechanism 25 at the corresponding position is taken out through the pulling mechanism 24, that is, the staff pushes the clamping block 29 to make the clamping block 29 away from the first clamping groove 31 at the corresponding position, and then pulls the pulling handle 32 to make the pulling plate 26 leave the fixing seat 27, thereby driving the fixing mechanism 25 out of the light-shielding cover 15, which is fast and convenient. The culture bottle on the fixing mechanism 25 at the corresponding position is taken out through the pulling mechanism 24, which is fast and convenient. And after detecting that the induction of the internal culture bottle fails, through the cooperation of the pulling mechanism 24 and the fixing mechanism 25, the contaminated culture bottle is quickly taken out to avoid direct contact, thereby reducing internal contamination. After the tissue culture is completed, the staff pulls the pulling mechanism 24 to take out the culture bottle fixed on the fixing mechanism 25, which is convenient for the staff to take out the peony plants propagated by tissue culture.

[0057] In summary, through the cooperation of the movable plate mechanism 3 and the light-shielding mechanism 7, a closed lightless environment is formed, and the inside is disinfected by the light-shielding mechanism 7 to ensure a sterile environment inside; The output shaft of the reduction motor 14 drives the rotation mechanism 44 to move, thereby driving several fixing mechanisms 25 to move and the culture bottles thereon to perform circular motion, avoiding the enrichment of metabolites, improving the proliferation uniformity, promoting the dedifferentiation of explants through low-speed rotation, shortening the callus induction cycle, enhancing oxygen penetration, and ensuring the induction and differentiation efficiency; The light-shielding mechanism 7 regularly detects the internal environment, the gas storage tank 6 quantitatively transports CO2 according to the detected data, the atomizing humidifier 5 quantitatively transports atomized water vapor according to the detected data, and in cooperation with the temperature controller 4, integrates the gas storage tank 6 and the atomizing humidifier 5 to form a photosynthetic-like environment with constant temperature, high humidity, and rich CO2 to ensure rapid induction of tissue culture; The light-shielding mechanism 7 provides supplementary light inside and, in cooperation with the rotation mechanism 44, dynamically adjusts the light intensity, light time, and light color to ensure the rapid propagation of peony flowers; The culture bottle is fixed by the fixing mechanism 25 to keep it stable when the culture bottle moves with the rotation mechanism 44, and in cooperation with the pulling mechanism 24, when the induction fails, the contaminated culture bottle is quickly taken out through the pulling mechanism 24, thereby reducing internal contamination and ensuring a clean culture environment.

[0058] The specific embodiments described herein are merely illustrative of the spirit of the present invention. Those skilled in the art to which the present invention pertains may make various modifications or supplements to the described specific embodiments or use similar means for substitution, but will not deviate from the spirit of the present invention or exceed the scope defined by the appended claims.

Claims

1. An induction device for rapid propagation of tissue-cultured peony flowers, comprising an air filter (1), a bracket mechanism (2), a temperature controller (4), an atomizing humidifier (5) and a gas storage tank (6) arranged in sequence from left to right, characterized in that, The support mechanism (2) is provided with a shading mechanism (7), the shading mechanism (7) is hinged with a movable plate mechanism (3), the support mechanism (2) is rotatably provided with a rotating shaft mechanism (8), the support mechanism (2) is provided with a reduction motor (14), the output shaft of the reduction motor (14) is transmission-connected to the rotating shaft mechanism (8), the rotating shaft mechanism (8) is provided with a rotating mechanism (44), and the rotating mechanism (44) is located inside the shading mechanism (7), and the rotating mechanism (44) is detachably provided with a plurality of pull-out groups, each of which consists of a plurality of pull-out groups. The temperature controller (4) is composed of a pull-out mechanism (24), each of which is provided with two fixing mechanisms (25) arranged symmetrically in front and back. The air inlet of the temperature controller (4) is connected to the gas storage tank (6) and the atomizing humidifier (5) respectively through a bifurcated pipeline, the air outlet of the temperature controller (4) is connected to the rotating shaft mechanism (8) through a pipeline, the shading mechanism (7) is connected to the air filter (1) through a pipeline, and a plurality of humidity sensors, a plurality of CO2 concentration sensors and a plurality of light sensors are provided inside the shading mechanism (7).

2. The induction device for rapid propagation of tissue-cultured peony flowers according to claim 1, characterized in that, The support mechanism (2) comprises a frame (9), a mounting seat (13) is provided on the side of the frame (9), and a reduction motor (14) is fixed on the mounting seat (13).

3. The induction device for rapid propagation of tissue-cultured peony flowers according to claim 2, characterized in that, The rotating mechanism (44) comprises two mounting frames (11) which are symmetrically arranged on both sides of the rotating shaft mechanism (8). The two mounting frames (11) are respectively arranged at two ends of the rotating shaft mechanism (8). Six balancing frames (12) which are evenly distributed in a circle are rotatably arranged on the mounting frames (11). The balancing frames (12) are in a triangular structure. The top of the balancing frame (12) is arranged on the mounting frame (11). The positions of the balancing frames (12) of the two mounting frames (11) correspond to each other. A placement plate (10) is detachably arranged between the two balancing frames (12) at corresponding positions of the two mounting frames (11), and the two ends of the placement plate (10) are respectively arranged on two sides of the bottom of the balancing frame (12) at the corresponding position.

4. The induction device for rapid propagation of tissue-cultured peony flowers according to claim 3, characterized in that, The shading mechanism (7) comprises two symmetrically arranged fixing plates (19), the two fixing plates (19) being fixed to both sides of the inner upper end of the frame (9), respectively; a shading cover (15) is fixed between the two fixing plates (19); a plurality of circumferentially evenly distributed ultraviolet disinfection lamps (17) and a plurality of circumferentially evenly distributed multi-color fill lights (18) and a plurality of cameras are arranged inside the shading cover (15); the plurality of multi-color fill lights (18) and the plurality of ultraviolet disinfection lamps (17) are arranged in an alternating manner; and an observation port (16) is provided on the shading cover (15).

5. An induction device for rapid propagation of tissue-cultured peony flowers according to claim 4, characterized in that, The movable plate mechanism (3) comprises a movable plate (22), an annular sealing gasket (21) is provided on the movable plate (22), the position of the annular sealing gasket (21) and the size of the inner ring correspond to the observation port (16), a plurality of equally spaced movable hinges (23) are provided on the upper end of the movable plate (22), the plurality of movable hinges (23) are all fixed on the light shield (15), electric push rods (20) are hinged on both sides of the rear end of the movable plate (22), and the ends of the electric push rods (20) are hinged in the light shield (15).

6. The induction device for rapid propagation of tissue-cultured peony flowers according to claim 5, characterized in that, The pulling mechanism (24) includes a fixed seat (27) and a pulling plate (26). Each fixed seat (27) is detachably arranged on the placing plate (10) at the corresponding position. On both sides inside the fixed seat (27), a first chute (28) is provided. At both ends of the upper end of the fixed seat (27), a second clamping groove is provided. Inside the second clamping groove, a clamping block (29) is rotatably arranged. On both sides of the pulling plate (26), a first slider (30) is fixed. The two first sliders (30) are symmetrically arranged. The position and size of the first slider (30) correspond to those of the first chute (28), and the first sliders (30) are all slidably arranged in the first chute (28) at the corresponding position. At the end of the pulling plate (26), a pulling handle (32) is provided. On both sides of the pulling handle (32), a first clamping groove (31) is provided. The position of the first clamping groove (31) corresponds to that of the second clamping groove, and the shape and size of the first clamping groove (31) correspond to those of the clamping block (29).

7. An induction device for rapid propagation of tissue-cultured peony flowers according to claim 6, characterized in that, The fixing mechanism (25) includes an electric base (35), a limiting ring (41) and a second slider (38). The limiting ring (41) is detachably arranged on the outer upper end of the electric base (35). The electric base (35) is detachably arranged on the pulling plate (26). On the electric base (35), a number of second chutes (37) are provided in a circumferentially uniform distribution. Inside the second chutes (37), the second sliders (38) are all slidably arranged. A return spring (36) is fixed between each of the second sliders (38) and the limiting ring (41). At the end of each second slider (38), a fixing rod (33) is provided. The fixing rod (33) is perpendicular to the corresponding second slider (38). At the end of the fixing rod (33), an arc-shaped guiding block (34) is provided. A number of fixing rods (33) cooperate with each other to form a fixing groove.

8. An induction device for rapid propagation of tissue-cultured peony flowers according to claim 7, characterized in that, The rotating shaft mechanism (8) includes a hollow rotating shaft (40). The hollow rotating shaft (40) is rotatably arranged on the upper end of the frame (9). On the hollow rotating shaft (40), a number of second ventilation holes (43) are provided in a circumferentially uniform distribution. Outside the hollow rotating shaft (40), a conveying pipe (42) is provided. On the conveying pipe (42), a number of first ventilation holes (39) are provided in a circumferentially uniform distribution. The first ventilation holes (39) and the second ventilation holes (43) are staggered and communicate with each other.

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