Immune cell cryopreservation cooling equipment and cooling method thereof

Abstract

The invention relates to the technical field of biomedical treatment, in particular to immune cell cryopreservation cooling equipment and a cooling method.The immune cell cryopreservation cooling equipment is characterized in that a swing chamber is arranged on a fixing frame, a storage barrel is installed in the swing chamber, a rotatable rotating disc is connected to the bottom of the storage barrel, the storage barrel and the rotating disc are connected in a swing mode, and a guide assembly is installed in the swing chamber; the driving device is used for driving the storage barrel to swing in the rotating process, a bearing disc is installed in the storage barrel, a plurality of bearing sleeves used for loading test tubes are arranged on the bearing disc and distributed in a circumferential array mode with the rotating axis of the storage barrel as the center, and abutting rails are installed at the bottoms of the bearing sleeves and arranged in the swing direction of the test tubes. The swing tracks of the test tubes at different positions are kept consistent, so that the mixing process of the cell solution and the cryopreservation liquid in each test tube is consistent, the cooling speed is the same, the cryopreservation quality is improved, and the cell survival rate is improved.

Description

Technical Field

[0001] The present invention relates to the field of biomedical technologies, and particularly to an immunocyte cryopreservation cooling device and a cooling method thereof. Background Art

[0002] Cell cryopreservation is to place healthy cells in an ultra-low temperature environment, so that the cells enter a temporary "dormant" state, reducing cell metabolism while not destroying the cell activity, for long-term storage. When needed, they can be resuscitated and applied in health care and disease intervention.

[0003] Before cryopreservation, the cell solution needs to be mixed with the cryopreservation solution, and then gradually cooled by liquid nitrogen until the cryopreservation temperature is reached. When the cryopreservation solution and the cell solution are mixed, rapid and uniform mixing is required, and at the same time, the oscillation degree of the mixing needs to be controlled to avoid cell damage. The Chinese invention patent with the application number CN202211341859.X discloses an immunocyte cryopreservation cooling device. By controlling the start of the motor, the rotating shaft and the rotating seat rotate, driving the test tube in the tank on the surface of the rotating seat to rotate. When the sliding sleeve slides on the surface of the transmission rod, it drives the hinged rod to move, driving the tank to tilt left and right on the rotating seat through the hinge ears and hinge seats at the bottom, facilitating the mixing efficiency of the cells and the cryopreservation solution in the test tube in further cooperation with the rotating state of the tank.

[0004] However, the applicant has found that the prior art has at least the following problems:

[0005] As Figure 1 shown, the tank sways left and right. The test tube on the same vertical line as the swaying axis swings along R1, and other test tubes swing along R2. The swinging angles of the test tubes are the same, but their swinging trajectories are different. The test tubes at the edge have a larger swinging amplitude, resulting in different mixing processes of the cells in each test tube, and possibly different cryopreservation effects of the cells in each test tube. Summary of the Invention

[0006] In view of this, the purpose of the present invention is to provide an immunocyte cryopreservation cooling device and a cooling method thereof to solve the problem that the mixing processes of the cells in each test tube are different, which may lead to different cryopreservation effects of the cells in each test tube.

[0007] For the above purposes, the present invention provides an immunocyte cryopreservation cooling device, which includes a support frame. A fixed frame is installed on the support frame, and a swing chamber is provided on the fixed frame. A storage cylinder is installed in the swing chamber. The bottom of the storage cylinder is connected to a rotatable turntable. The storage cylinder is swingably connected to the turntable. A guiding component is installed in the swing chamber for driving the storage cylinder to swing during rotation. A bearing plate is installed in the storage cylinder, and multiple bearing sleeves for loading test tubes are arranged on the bearing plate. The multiple bearing sleeves are distributed in a circumferential array centered on the rotation axis of the storage cylinder. The bottom of the bearing sleeve is open, and a slot is provided at the open end. A contact track is installed in the slot, and the contact track is arranged along the swing direction of the test tube. The bottom of the contact track is connected to a connecting rod, and the bottom of the connecting rod is fixedly connected to the turntable. An opening is provided at the bottom of the storage cylinder, and a sealing hose is connected between the opening and the turntable. The top of the storage cylinder is detachably installed with a cylinder cover. Multiple test tube plug components are elastically arranged at the bottom of the cylinder cover, and the test tube plug components correspond to the test tubes one by one. The storage cylinder is connected with a liquid nitrogen inlet, a liquid nitrogen outlet and a thermometer.

[0008] After mixing the cell solution and the cryopreservation solution in the test tubes, place multiple test tubes in the bearing sleeves respectively. Then close the cylinder cover, and the multiple test tube plug components at the bottom of the cylinder cover correspond to the multiple test tubes one by one. Press the test tubes tightly against the contact track. Then, under the rotation of the turntable, drive the storage cylinder to rotate. During the rotation of the storage cylinder, since a guiding component is arranged inside the swing chamber, the guiding component cooperates with the rotation action of the storage cylinder to push the storage cylinder to swing, so that the test tubes inside the storage cylinder swing while rotating around the turntable as the axis, thereby improving the mixing efficiency of the cell solution and the cryopreservation solution. During the movement of the multiple test tubes along with the storage cylinder, since they are distributed in a circumferential array centered on the rotation axis of the storage cylinder, their rotational movements in the horizontal direction are basically the same. In the swinging movement in the vertical direction, due to the test tube plug components holding the test tubes, the test tubes keep in contact with the contact track, and their movement amplitudes in the vertical direction are the same, thus ensuring that the mixing processes of the cell solution and the cryopreservation solution in each test tube are consistent. During the mixing process, liquid nitrogen is introduced into the storage cylinder for cooling, so that the cryopreservation states of the cells in each test tube are the same, that is, the mixing processes are consistent and the cooling speeds are the same, thereby improving the cryopreservation quality and the cell survival rate.

[0009] Optionally, the multiple bearing sleeves include edge bearing sleeves and middle bearing sleeve groups. The two middle bearing sleeve groups are arranged directly above the swing axis of the bearing plate. The bottom of the middle bearing sleeve is sealed, and the shape of the contact track provided at the bottom of the edge bearing sleeve is the same as the swing track of the inner bottom surface of the middle bearing sleeve.

[0010] Optionally, the test tube plug component includes a mounting post fixedly connected to the cylinder cover. The mounting post is movably connected with a pressing plug that matches the test tube. A telescopic hole adapted to the mounting post is provided on the pressing plug, and a return spring is also sleeved on the mounting post.

[0011] Optionally, a receiving post is provided at the center of the carrier plate. The receiving post is in the shape of a hexagonal prism, and receivers are respectively installed on six faces of the receiving post. A plurality of receivers are respectively opposite to a plurality of test tubes one by one. A light emitter corresponding to the receiver is provided on the inner wall of the storage cylinder. The light emitter is used to emit a light signal through the test tube, and the receiver is used to receive the light signal emitted by the light emitter. A plurality of the receivers are electrically connected to a determination module. The determination module is used to determine whether the receiver receives the light signal emitted by its corresponding light emitter. The determination module is electrically connected to the rotating disk and the guiding component. When the determination module determines that the receiver does not receive the light signal, it controls the rotating disk to reduce the rotation speed and controls the guiding component to move to reduce the swing amplitude of the storage cylinder until the receiver receives the light signal again; conversely, when the determination module determines that the receiver receives the light signal, it controls the rotating disk to increase the rotation speed and controls the guiding component to move to increase the swing amplitude of the storage cylinder until the receiver cannot receive the light signal.

[0012] Optionally, the support frame is connected to a fixed rod, the fixed rod is connected to a base, a circular rotating groove is provided on the base, a rotating disk is adaptively installed inside the rotating groove, and a convex disk adapted to the rotating groove is provided at the edge of the rotating disk. The bottom of the base is connected to a fixed arm, the fixed arm is connected to a motor base, a rotating motor is fixedly installed in the motor base, and the rotating motor is fixedly connected to a driving rotating shaft, and the driving rotating shaft is fixedly connected to the rotating disk.

[0013] Optionally, two sets of shaft seats are fixedly installed on the rotating disk, a swinging rotating shaft is installed between the two sets of shaft seats, the swinging rotating shaft is fixedly connected to a fixed block, and the fixed block is fixedly connected to the storage cylinder.

[0014] Optionally, an adjustment groove is opened on the inner wall of the swinging chamber. The guiding component includes a limiting ring adaptively installed in the adjustment groove. Two deflection telescopic rods are respectively installed at both ends inside the adjustment groove. The two deflection telescopic rods are used to drive the limiting ring to move along the adjustment groove. Elastic telescopic rods are respectively connected to the side walls on both sides of the swinging direction of the storage cylinder, and the elastic telescopic rods respectively abut against the limiting ring.

[0015] Optionally, the end of the elastic telescopic rod is inserted into the adjustment groove. A ball is installed at the end of the elastic telescopic rod in contact with the limiting ring. The other end of the elastic telescopic rod is connected to a U-shaped block, a sliding buckle is installed on the U-shaped block, and a guide rail groove adapted to the sliding buckle is opened on a guide ear fixedly installed on the side wall of the storage cylinder.

[0016] The present invention also provides an immune cell cryopreservation cooling method, including:

[0017] Step 1: Mix the cell solution and the cryopreservation solution and load them into test tubes;

[0018] Step 2: Place the test tubes inside the storage cylinder respectively and tighten the cylinder cover;

[0019] Step 3: The rotary motor is started to drive the storage cylinder to rotate, and liquid nitrogen is introduced into the storage cylinder to cool it down;

[0020] Step 4: The determination module determines the reception of the optical signal, and changes the rotation speed of the rotating motor and the guide track of the guide component according to the determination result, so as to control the mixing oscillation amplitude of the solution in the test tube;

[0021] Step 5: The thermometer monitors the internal temperature of the storage cylinder. When the temperature reaches the set temperature, the liquid nitrogen inlet is closed.

[0022] Optionally, the determination module determines the reception of the optical signal and changes the rotation speed of the rotating motor and the guide track of the guide component according to the determination result, so as to control the mixing oscillation amplitude of the solution in the test tube, including:

[0023] The determination module is used to determine whether the receiver has received the light signal emitted by its corresponding light transmitter. The determination module is electrically connected to the rotating disk and the guide assembly. When the determination module determines that the receiver has not received the light signal, the rotating disk is controlled to reduce the rotation speed, and the guide assembly is controlled to move to reduce the swing amplitude of the storage cylinder until the receiver receives the light signal again; conversely, when the determination module determines that the receiver has received the light signal, the rotating disk is controlled to increase the rotation speed, and the guide assembly is controlled to move to increase the swing amplitude of the storage cylinder until the receiver does not receive the light signal.

[0024] The beneficial effects of the present invention are as follows: by pressing the test tube against the abutment track, the storage barrel is driven to rotate under the rotation of the rotating disk. During the rotation of the storage barrel, a guide assembly is provided inside the swing chamber. The guide assembly cooperates with the rotation of the storage barrel to push the storage barrel to swing, so that the test tube inside the storage barrel swings while rotating with the rotating disk as the axis, thereby improving the mixing efficiency of the cell solution and the freezing solution. In the process of the movement of the storage barrel, the multiple test tubes are distributed in a circular array with the rotation axis of the storage barrel as the center, so the rotational movement in the horizontal direction is basically consistent. In the vertical swinging movement, the test tube plug assembly abuts the test tube so that the test tube keeps in contact with the abutment track, and the movement amplitude in the vertical direction is consistent, thereby ensuring that the mixing process of the cell solution and the freezing solution in each test tube is consistent. During the mixing process, liquid nitrogen is introduced into the storage barrel for cooling, so that the freezing state of the cells in each test tube is consistent, that is, the mixing process is consistent and the cooling speed is the same, thereby improving the freezing quality and the cell survival rate. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] To more clearly illustrate the technical solutions in the present invention or the prior art, the following will briefly introduce the drawings required for the description of the embodiments or the prior art. Obviously, the drawings in the following description are only those of the present invention. For those of ordinary skill in the art, without creative efforts, other drawings can also be obtained based on these drawings.

[0026] Figure 1 Schematic working principle diagram of the comparative document;

[0027] Figure 2 Stereogram of an immune cell cryopreservation and cooling device according to an embodiment of the present invention;

[0028] Figure 3 Partial cross-sectional schematic diagram of an immune cell cryopreservation and cooling device according to an embodiment of the present invention;

[0029] Figure 4 Partial cross-sectional schematic diagram when an immune cell cryopreservation and cooling device according to an embodiment of the present invention is working;

[0030] Figure 5 Schematic diagram of the storage cylinder of an immune cell cryopreservation and cooling device according to an embodiment of the present invention Figure 1 ;

[0031] Figure 6 Schematic diagram of the storage cylinder of an immune cell cryopreservation and cooling device according to an embodiment of the present invention Figure 2 ;

[0032] Figure 7 Partial cross-sectional schematic diagram of the storage cylinder of an immune cell cryopreservation and cooling device according to an embodiment of the present invention Figure 1 ;

[0033] Figure 8 Partial cross-sectional schematic diagram of the storage cylinder of an immune cell cryopreservation and cooling device according to an embodiment of the present invention Figure 2 ;

[0034] Figure 9 Cross-sectional schematic diagram of the storage cylinder of an immune cell cryopreservation and cooling device according to an embodiment of the present invention;

[0035] Figure 10 Cross-sectional schematic diagram of the storage cylinder when an immune cell cryopreservation and cooling device according to an embodiment of the present invention is working;

[0036] Figure 11 Transverse cross-sectional schematic diagram of the storage cylinder of an immune cell cryopreservation and cooling device according to an embodiment of the present invention. The markings in the figure are:

[0037] 101, Support frame; 102, Fixed frame; 103, Adjustment groove; 104, Limiting ring; 105, Deflection telescopic rod; 106, Fixed rod; 107, Base; 108, Fixed arm; 109, Motor base; 110, Rotating motor; 111, Driving rotating shaft; 112, Rotating disk; 113, Protruding disk; 114, Swing chamber; 201, Storage cylinder; 202, Cylinder cover; 203, Fixed block; 204, Axle seat; 205, Swing rotating shaft; 206, Guide rail groove; 207, U-shaped block; 208, Elastic telescopic rod; 209, Guide ear; 301, Carrying disk; 302, Edge carrying sleeve; 303, Test tube; 304, Groove; 305, Test tube plug assembly; 3051, Tightening plug; 3052, Mounting post; 3053, Return spring; 306, Middle carrying sleeve; 401, Contact track; 402, Connecting rod; 403, Opening; 404, Sealing hose; 501, Receiving post; 502, Receiver; 503, Light emitter. Detailed implementation manner

[0038] To make the objectives, technical solutions and advantages of the present invention clearer and more understandable, the present invention will be further described in detail below in conjunction with specific embodiments.

[0039] It should be noted that unless otherwise defined, the technical terms or scientific terms used in the present invention should have the ordinary meanings understood by those with ordinary skills in the field to which the present invention belongs. The "first", "second" and similar terms used in the present invention do not indicate any order, quantity or importance, but are only used to distinguish different components. The terms such as "including" or "comprising" mean that the elements or objects appearing before this word cover the elements or objects listed after this word and their equivalents, without excluding other elements or objects. The terms such as "connected" or "linked" are not limited to physical or mechanical connections, but may include electrical connections, whether direct or indirect. The terms such as "upper", "lower", "left" and "right" are only used to represent relative position relationships. When the absolute position of the object being described changes, the relative position relationship may also change accordingly.

[0040] As Figures 2 to 11 shown, a specific embodiment of the present invention provides an immune cell cryopreservation cooling device, which includes a support frame 101. A fixed frame 102 is installed on the support frame 101. A swing chamber 114 is provided on the fixed frame 102. A storage cylinder 201 is installed in the swing chamber 114. The bottom of the storage cylinder 201 is connected to a rotatable rotating disk 112. The storage cylinder 201 is swingably connected to the rotating disk 112. A guiding component is installed in the swing chamber 114 for driving the storage cylinder 201 to swing during rotation;

[0041] A bearing plate 301 is installed in the storage cylinder 201. Multiple bearing sleeves for loading test tubes 303 are arranged on the bearing plate 301. The multiple bearing sleeves are distributed in a circumferential array centered on the rotation axis of the storage cylinder 201. The bottom of the bearing sleeve is open, and a slot 304 is provided at the open end. A contact track 401 is installed in the slot 304. The contact track 401 is arranged along the swinging direction of the test tube 303. The bottom of the contact track 401 is connected to a connecting rod 402. The bottom of the connecting rod 402 is fixedly connected to the rotating disk 112. An opening 403 is formed at the bottom of the storage cylinder 201. A sealing hose 404 is connected between the opening 403 and the rotating disk 112. A cylinder cover 202 is detachably installed at the top of the storage cylinder 201. A plurality of test tube plug assemblies 305 are elastically arranged at the bottom of the cylinder cover 202. The test tube plug assemblies 305 correspond to the test tubes 303 one by one. The storage cylinder 201 is connected with a liquid nitrogen inlet, a liquid nitrogen outlet and a thermometer.

[0042] After mixing the cell solution and the cryopreservation solution in the test tube 303, the multiple test tubes 303 are respectively placed in the bearing sleeves. Then the cylinder cover 202 is closed. The multiple test tube plug assemblies 305 at the bottom of the cylinder cover 202 respectively correspond to the multiple test tubes 303 one by one. The test tube 303 is pressed tightly against the contact track 401. Then, under the rotation of the rotating disk 112, the storage cylinder 201 is driven to rotate. During the rotation of the storage cylinder 201, since a guiding component is arranged inside the swinging chamber 114, the guiding component cooperates with the rotation action of the storage cylinder 201 to push the storage cylinder 201 to swing, so that the test tubes 303 inside the storage cylinder 201 rotate around the rotating disk 112 as the axis and swing at the same time, thereby improving the mixing efficiency of the cell solution and the cryopreservation solution. During the movement of the multiple test tubes 303 along with the storage cylinder 201, since they are distributed in a circumferential array centered on the rotation axis of the storage cylinder 201, their rotational movements in the horizontal direction are basically the same. In the swinging movement in the vertical direction, since the test tube plug assemblies 305 hold the test tubes 303, the test tubes 303 keep in contact with the contact track 401, and their movement amplitudes in the vertical direction are the same, so as to ensure that the mixing processes of the cell solution and the cryopreservation solution in each test tube 303 are the same. During the mixing process, liquid nitrogen is introduced into the storage cylinder 201 for cooling, so that the cryopreservation states of the cells in each test tube 303 are the same, that is, the mixing processes are the same and the cooling speeds are the same, thereby improving the cryopreservation quality and the cell survival rate.

[0043] In some optional specific embodiments, such as Figures 7 to 10As shown, multiple said bearing sleeves include 4 edge bearing sleeves 302 and 2 groups of middle bearing sleeves 306. The two groups of middle bearing sleeves 306 are arranged directly above the swing axis of the bearing disc 301. The bottom of the middle bearing sleeve 306 is sealed. The shape of the contact track 401 provided at the bottom of the edge bearing sleeve 302 is the same as the swing track of the inner bottom surface of the middle bearing sleeve 306. During use, since the test tube 303 in the middle bearing sleeve 306 only has a swinging action and no movement in the up and down directions, the mixing in the middle bearing sleeve 306 is more appropriate. By providing the contact track 401 at the bottom of the edge bearing sleeve 302, the mixing actions of multiple test tubes 303 are kept highly consistent.

[0044] In some optional specific embodiments, such as Figure 9 and Figure 10 As shown, the test tube plug assembly 305 includes a mounting post 3052 fixedly connected to the cylinder cover 202. The mounting post 3052 is movably connected with a tightening plug 3051 that cooperates with the test tube 303. The tightening plug 3051 is provided with a telescopic hole adapted to the mounting post 3052. A return spring 3053 is also sleeved on the mounting post 3052. Keeping the test tube 303 tightened, on the one hand, it prevents the solution from leaking, and on the other hand, it makes the bottom of the test tube 303 closely adhere to the contact track 401.

[0045] In some optional specific embodiments, such as Figure 11As shown in the figure, a receiving post 501 is provided at the center of the carrier plate 301. The receiving post 501 is in the shape of a hexagonal prism. Receivers 502 are respectively installed on the six faces of the receiving post 501. A plurality of receivers 502 are respectively opposite to a plurality of test tubes 303 one by one. On the inner wall of the storage cylinder 201, there is a light emitter 503 corresponding to the receiver 502. The light emitter 503 is used to emit a light signal through the test tube 303. The receiver 502 is used to receive the light signal emitted by the light emitter 503. A plurality of the receivers 502 are electrically connected to a determination module. The determination module is used to judge whether the receiver 502 receives the light signal emitted by its corresponding light emitter 503. The determination module is electrically connected to the rotating disk 112 and the guiding component. When the determination module determines that the receiver 502 does not receive the light signal, it controls the rotating disk 112 to reduce the rotation speed and controls the guiding component to move to reduce the swing amplitude of the storage cylinder 201 until the receiver 502 receives the light signal again; conversely, when the determination module determines that the receiver 502 receives the light signal, it controls the rotating disk 112 to increase the rotation speed and controls the guiding component to move to increase the swing amplitude of the storage cylinder 201 until the receiver 502 cannot receive the light signal. In order to keep the cells in the test tube 303 undamaged and enable the cell solution to be mixed with the cryogenic liquid as soon as possible, a light signal is emitted by the light emitter 503, passes through the test tube 303, and is received by the receiver 502. When the swing amplitude of the test tube 303 will not cause cell damage, the oscillation amplitude of the solution in the test tube 303 is small. Therefore, the light signal only passes through the transparent inner wall of the test tube 303, and the light receiver 502 receives the light signal. When the swing amplitude of the cell solution in the test tube 303 is too large, the oscillation amplitude of the solution is large and refracts the light signal, causing the light signal to deflect, and the receiver 502 does not receive the light signal. Thus, the dynamic adjustment of the swing amplitude of the storage cylinder 201 is realized, so that the solution in the test tube 303 maintains an appropriate mixing rate.

[0046] In some optional specific embodiments, such as Figures 2 to 9 As shown in the figure, the support frame 101 is connected with a fixed rod 106. The fixed rod 106 is connected with a base 107. A circular rotating groove is provided on the base 107. A rotating disk 112 is adaptively installed inside the rotating groove. A convex disk 113 adapted to the rotating groove is provided at the edge of the rotating disk 112. The bottom of the base 107 is connected with a fixed arm 108. The fixed arm 108 is connected with a motor base 109. A rotating motor 110 is fixedly installed in the motor base 109. The rotating motor 110 is fixedly connected with a driving rotating shaft 111. The driving rotating shaft 111 is fixedly connected with the rotating disk 112. During operation, the rotating motor 110 drives the driving rotating shaft 111 to rotate. The rotation of the driving rotating shaft 111 drives the rotating disk 112 to rotate in the base 107, thereby driving the storage cylinder 201 to rotate.

[0047] In some optional specific embodiments, such as Figures 5 to 10As shown, two sets of shaft seats 204 are fixedly installed on the rotating disk 112. A swinging rotating shaft 205 is installed between the two sets of shaft seats 204. The swinging rotating shaft 205 is fixedly connected to a fixing block 203, and the fixing block 203 is fixedly connected to the storage cylinder 201.

[0048] In some optional specific embodiments, such as Figures 2 to 6 As shown, an adjustment groove 103 is formed on the inner wall of the swinging chamber 114. The guiding assembly includes a limiting ring 104 fitted in the adjustment groove 103. Deflection telescopic rods 105 are respectively installed at both ends inside the adjustment groove 103. The two sets of deflection telescopic rods 105 are used to drive the limiting ring 104 to move along the adjustment groove 103. Elastic telescopic rods 208 are respectively connected to the side walls on both sides of the swinging direction of the storage cylinder 201, and the elastic telescopic rods 208 are respectively abutted against the limiting ring 104. When the center of the limiting ring 104 is concentric with the rotation axis of the rotating disk 112, at this time, when the rotating disk 112 rotates, it will not drive the storage cylinder 201 to swing. However, when the two sets of deflection telescopic rods 105 cooperate to expand and contract, so that the center of the limiting ring 104 is offset from the rotation axis of the rotating disk 112, then when the rotating disk 112 rotates, the storage cylinder 201 swings around the swinging rotating shaft 205 as the axis, and the swinging amplitude can be adjusted by adjusting the offset distance between the center of the limiting ring 104 and the rotation axis of the rotating disk 112.

[0049] In some optional specific embodiments, such as Figures 2 to 6 As shown, the end of the elastic telescopic rod 208 is inserted into the adjustment groove 103. A ball is installed at the end of the elastic telescopic rod 208 in contact with the limiting ring 104. The other end of the elastic telescopic rod 208 is connected to a U-shaped block 207. A sliding buckle is installed on the U-shaped block 207. A guiding ear 209 is fixedly installed on the side wall of the storage cylinder 201, and a guide rail groove 206 adapted to the sliding buckle is formed on the guiding ear 209. During use, the friction between the elastic telescopic rod 208 and the limiting ring 104 is reduced by the ball. At the same time, the elastic telescopic rod 208 is limited by the adjustment groove 103, and the elastic telescopic rod 208 is movably connected to the storage cylinder 201 through the mutual cooperation between the sliding buckle and the guiding ear 209.

[0050] According to an immune cell cryopreservation cooling device provided above, the present invention also provides an immune cell cryopreservation cooling method, including:

[0051] Step 1: Mix the cell solution and the cryopreservation solution and load them into the test tube 303;

[0052] Step 2: Place the test tube 303 inside the storage cylinder 201 respectively, and tighten the cylinder cover 202;

[0053] Step 3: Start the rotation motor 110, drive the storage cylinder 201 to rotate, and introduce liquid nitrogen into the storage cylinder 201 for cooling;

[0054] Step Four: The determination module determines the reception of the optical signal and changes the rotation speed of the rotary motor 110 and the guiding trajectory of the guiding component according to the determination result, so as to control the mixing and oscillation amplitude of the solution in the test tube 303;

[0055] Step Five: The thermometer monitors the internal temperature of the storage cylinder 201. When the temperature reaches the set temperature, the liquid nitrogen inlet is closed.

[0056] In some optional specific embodiments, the determination module determines the reception of the optical signal and changes the rotation speed of the rotary motor 110 and the guiding trajectory of the guiding component according to the determination result, so as to control the mixing and oscillation amplitude of the solution in the test tube 303, including:

[0057] The determination module is used to judge whether the receiver 502 receives the optical signal emitted by its corresponding optical transmitter 503. The determination module is electrically connected to the rotating disk 112 and the guiding component. When the determination module determines that the receiver 502 does not receive the optical signal, it controls the rotating disk 112 to reduce the rotation speed and controls the guiding component to move, reducing the swing amplitude of the storage cylinder 201 until the receiver 502 receives the optical signal again; conversely, when the determination module determines that the receiver 502 receives the optical signal, it controls the rotating disk 112 to increase the rotation speed and controls the guiding component to move, increasing the swing amplitude of the storage cylinder 201 until the receiver 502 cannot receive the optical signal.

[0058] The working principle of the present invention is as follows: after the cell solution and the cryopreservation solution are mixed in the test tube 303, the multiple test tubes 303 are placed in the carrying sleeve respectively, and then the cylinder cover 202 is closed, and the multiple test tube plug assemblies 305 at the bottom of the cylinder cover 202 correspond to the multiple test tubes 303 one by one, respectively, and the test tubes 303 are pressed tightly against the contact track 401, and then the storage cylinder 201 is driven to rotate under the rotation of the rotating disk 112. During the rotation of the storage cylinder 201, since a guide assembly is provided inside the swing chamber 114, the guide assembly cooperates with the rotation action of the storage cylinder 201 to push the storage cylinder 201 to swing, so that the test tubes 303 inside the storage cylinder 201 swing while rotating with the rotating disk 112 as the axis, thereby improving the cell solution and the cryopreservation solution. Mixing efficiency, and multiple test tubes 303 are distributed in a circular array with the rotation axis of the storage cylinder 201 as the center during the movement with the storage cylinder 201, so their rotational motion in the horizontal direction is basically consistent. In the vertical swinging motion, the test tube plug assembly 305 supports the test tube 303, so that the test tube 303 keeps in contact with the abutment track 401, and the vertical movement amplitude remains consistent, thereby ensuring that the mixing process of the cell solution and the freezing solution in each test tube 303 is consistent. During the mixing process, liquid nitrogen is introduced into the storage cylinder 201 for cooling, so that the freezing state of the cells in each test tube 303 is consistent, that is, the mixing process is consistent and the cooling rate is the same, thereby improving the freezing quality and the cell survival rate.

[0059] Those skilled in the art should understand that the discussion of any of the above embodiments is merely illustrative and is not intended to imply that the scope of the present invention (including the claims) is limited to these examples. Under the concept of the present invention, the technical features in the above embodiments or different embodiments may be combined, the steps may be implemented in any order, and there are many other variations of the different aspects of the present invention as described above, which are not provided in detail for the sake of simplicity.

[0060] The present invention is intended to cover all such substitutions, modifications and variations that fall within the broad scope of the appended claims. Therefore, any omissions, modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the scope of protection of the present invention.

Claims

1. An immune cell freezing and cooling device, comprising a support frame (101), a fixed frame (102) is mounted on the support frame (101), a swing chamber (114) is provided on the fixed frame (102), a storage cylinder (201) is mounted in the swing chamber (114), a rotatable rotating disk (112) is connected to the bottom of the storage cylinder (201), the storage cylinder (201) and the rotating disk (112) are swingably connected, a guide assembly is mounted in the swing chamber (114) for driving the storage cylinder (201) to swing during the rotation process, characterized in that: A carrier plate (301) is installed in the storage cylinder (201), and a plurality of carrier sleeves for loading the test tubes (303) are arranged on the carrier plate (301), and the plurality of carrier sleeves are arranged in a circular array with the rotation axis of the storage cylinder (201) as the center, and the bottom of the carrier sleeve is open, and a slot (304) is arranged at the open position, and a friction track (401) is installed in the slot (304), and the friction track (401) is arranged along the swinging direction of the test tube (303), and the bottom of the friction track (401) is connected to a connecting rod (402), and the connecting rod (402) is connected to the bottom of the friction track (401). The bottom of the rod (402) is fixedly connected to the rotating disk (112); an opening (403) is provided at the bottom of the storage cylinder (201); a sealing hose (404) is connected between the opening (403) and the rotating disk (112); a cylinder cover (202) is detachably mounted on the top of the storage cylinder (201); a plurality of test tube plug assemblies (305) are elastically arranged at the bottom of the cylinder cover (202); the test tube plug assemblies (305) correspond to the test tubes (303) one by one; and the storage cylinder (201) is connected to a liquid nitrogen inlet, a liquid nitrogen outlet, and a thermometer.

2. The immune cell freezing and cooling device according to claim 1, characterized in that: The multiple bearing sleeves include (4) edge bearing sleeves (302) and (2) groups of middle bearing sleeves (306). The two groups of middle bearing sleeves (306) are arranged directly above the swing axis of the bearing plate (301). The bottom of the middle bearing sleeve (306) is sealed. The shape of the interference track (401) provided at the bottom of the edge bearing sleeve (302) is the same as the swing track of the inner bottom surface of the middle bearing sleeve (306).

3. The immune cell freezing and cooling device according to claim 1, characterized in that: The test tube plug assembly (305) comprises a mounting post (3052) fixedly connected to the cylinder cover (202); the mounting post (3052) is movably connected to a tight plug (3051) matched with the test tube (303); a telescopic hole matched with the mounting post (3052) is provided on the tight plug (3051); and a return spring (3053) is also sleeved on the mounting post (3052).

4. The immune cell cryopreservation and cooling device according to claim 1, characterized in that: A receiving column (501) is provided at the center of the carrier plate (301), the receiving column (501) is in the shape of a hexagonal prism, and receivers (502) are respectively installed on the six faces of the receiving column (501), and the multiple receivers (502) are respectively opposite to the multiple test tubes (303), and a light emitter (503) corresponding to the receiver (502) is provided on the inner wall of the storage cylinder (201), the light emitter (503) is used to emit a light signal to pass through the test tube (303), and the receiver (502) is used to receive the light signal emitted by the light emitter (503), and the multiple receivers (502) are electrically connected to a determination module, and the determination module is used to determine whether the receiver (502) The determination module is electrically connected to the rotating disk (112) and the guide assembly to determine whether the optical signal emitted by the corresponding optical transmitter (503) is received. When the determination module determines that the receiver (502) has not received the optical signal, the rotating disk (112) is controlled to reduce the rotation speed, and the guide assembly is controlled to move to reduce the swing amplitude of the storage cylinder (201) until the receiver (502) receives the optical signal again. On the contrary, when the determination module determines that the receiver (502) has received the optical signal, the rotating disk (112) is controlled to increase the rotation speed, and the guide assembly is controlled to move to increase the swing amplitude of the storage cylinder (201) until the receiver (502) does not receive the optical signal.

5. The immune cell cryopreservation and cooling device according to claim 1, characterized in that: The support frame (101) is connected to a fixing rod (106), the fixing rod (106) is connected to a base (107), a circular rotating groove is provided on the base (107), a rotating disk (112) is adapted to be installed inside the rotating groove, a raised disk (113) adapted to the rotating groove is provided at the edge of the rotating disk (112), a fixing arm (108) is connected to the bottom of the base (107), the fixing arm (108) is connected to a motor base (109), a rotating motor (110) is fixedly installed in the motor base (109), the rotating motor (110) is fixedly connected to a driving shaft (111), and the driving shaft (111) is fixedly connected to the rotating disk (112).

6. The immune cell cryopreservation and cooling device according to claim 1, characterized in that: Two groups of shaft seats (204) are fixedly mounted on the rotating disk (112), a swing shaft (205) is mounted between the two groups of shaft seats (204), the swing shaft (205) is fixedly connected to a fixed block (203), and the fixed block (203) is fixedly connected to the storage cylinder (201).

7. The immune cell cryopreservation and cooling device according to claim 1, characterized in that: An adjustment groove (103) is provided on the inner wall of the swing chamber (114); the guide assembly comprises a limit ring (104) adapted to be installed in the adjustment groove (103); deflection telescopic rods (105) are respectively installed at both ends of the adjustment groove (103); the two groups of deflection telescopic rods (105) are used to drive the limit ring (104) to move along the adjustment groove (103); elastic telescopic rods (208) are respectively connected to the side walls on both sides of the swing direction of the storage cylinder (201); the elastic telescopic rods (208) are respectively abutted against the limit ring (104).

8. The immune cell cryopreservation and cooling device according to claim 7, characterized in that: The end of the elastic telescopic rod (208) is inserted into the adjustment groove (103), and a ball is installed at the end of the elastic telescopic rod (208) that contacts the limit ring (104). The other end of the elastic telescopic rod (208) is connected to a U-shaped block (207), and a sliding buckle is installed on the U-shaped block (207). A guide ear (209) is fixedly installed on the side wall of the storage cylinder (201), and a guide rail groove (206) adapted to the sliding buckle is opened on the guide ear (209).

9. The cooling method of the immune cell cryopreservation cooling device according to any one of claims 1 to 8, characterized in that: The steps include: Mix the cell solution and the freezing solution and put them into a test tube (303); Place the test tubes (303) containing the cell solution and the freezing solution in the storage tube (201) respectively, and cover the tube cover (202) tightly; The rotating motor (110) is started to drive the storage cylinder (201) to rotate, and liquid nitrogen is introduced into the storage cylinder (201) to reduce the temperature; The determination module determines the reception of the optical signal and changes the rotation speed of the rotating motor (110) and the guide track of the guide component according to the determination result, so as to control the mixing oscillation amplitude of the solution in the test tube (303); The thermometer monitors the internal temperature of the storage cylinder (201), and when the temperature reaches a set temperature, the liquid nitrogen inlet is closed.

10. The immune cell cryopreservation and cooling method according to claim 9, characterized in that: The determination module determines the reception of the optical signal and changes the rotation speed of the rotating motor (110) and the guide track of the guide component according to the determination result, so as to control the mixing oscillation amplitude of the solution in the test tube (303), including: The determination module is used to determine whether the receiver (502) receives the optical signal emitted by its corresponding optical transmitter (503). The determination module is electrically connected to the rotating disk (112) and the guide component. When the determination module determines that the receiver (502) does not receive the optical signal, the rotating disk (112) is controlled to reduce the rotation speed, and the guide component is controlled to move to reduce the swing amplitude of the storage cylinder (201) until the receiver (502) receives the optical signal again. On the contrary, when the determination module determines that the receiver (502) receives the optical signal, the rotating disk (112) is controlled to increase the rotation speed, and the guide component is controlled to move to increase the swing amplitude of the storage cylinder (201) until the receiver (502) does not receive the optical signal.

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