Bactericidal composition and application thereof

Abstract

The invention relates to a bactericidal composition and application thereof, the bactericidal composition comprises an active component A and an active component B. The active component A is a compound represented by formula (I), the active component B is any one of Faneptadoquin and Fanpyramid, and the mass ratio of the active component A to the active component B is 1: 36-20: 1. The bactericidal composition or the preparation thereof disclosed by the invention has a remarkable synergistic effect on preventing and treating diseases caused by various fungi, the preventing and treating effect is obviously higher than that of a single component, the lasting period is long, meanwhile, the development of drug resistance can be delayed, and the bactericidal composition or the preparation thereof is pollution-free to the environment and safe to natural enemies and crops.

Description

Technical Field

[0001] The present invention relates to the field of agricultural fungicides, and particularly relates to a bactericidal composition and its application. Background Art

[0002] Bifemetstrobine is a strobilurin fungicide developed by Sumitomo Chemical Co., Ltd. with CAS registration number: 2454319-63-0. The Chinese chemical name is methyl (2Z)-3-methoxy-2-[(4-methyl[1,1'-biphenyl]-3-yl)oxy]prop-2-enoate, and the chemical structure is as follows:

[0003]

[0004] Feneptamidoquin is a quinolinecarboxamide fungicide developed by Syngenta. It has a broad control spectrum, is safe and highly effective, and can effectively control a variety of fungal diseases. CAS registration number: 2132414-06-1 (racemic 2132414-04-9), and the English chemical name is N-(2R)-[1,3-dimethyl-1-(phenylmethyl)butyl]-8-fluoro-3-quinolinecarboxamide, and the chemical structure is as follows:

[0005]

[0006] Fenopyramid is a pyrazolecarboxamide fungicide developed by Shandong Zhongnong United Biotechnology Co., Ltd. with CAS registration number: 2344721-61-3. The Chinese chemical name is N-{2-[2,4-bis(trifluoromethyl)phenoxy]phenyl}-3-(difluoromethyl)-1-methyl-1H-pyrazole-4-carboxamide, and the chemical structure is as follows:

[0007]

[0008] At present, chemical pesticides are still the main force in preventing and controlling crop diseases. However, the long-term use of pesticide fungicides will cause cross-resistance, resulting in a significant reduction in the control effect. The main means to solve this problem is to compound two or more pesticide fungicides to produce a synergistic effect and improve the efficacy. In addition, the combination of the compound of formula (I) with Feneptamidoquin and Fenopyramid has not been reported. Summary of the Invention

[0009] Based on the above situation, the object of the present invention is to provide a bactericidal composition and its preparation, which are mainly used for preventing and controlling plant fungal diseases. The composition or its preparation can enhance the drug efficacy, reduce the dosage of the drug, have a significant synergistic effect under a certain mass ratio, and at the same time can extend the effective period and delay the development of drug resistance.

[0010] In order to achieve the above object, the following technical solution is provided: A bactericidal composition contains active ingredient A and active ingredient B. The active ingredient A is a compound represented by formula (I), and the chemical structural formula is:

[0011] The active ingredient B is any one of Feneptamidoquin and Fenopyramid;

[0012] Further, the mass ratio of the active ingredient A to the active ingredient B is 1:36 to 20:1, or any value between the above values;

[0013] Further, the mass ratio of the active ingredient A to the active ingredient B is 1:24 to 20:1;

[0014] Further, the mass ratio of the compound of formula (I) to Feneptamidoquin is 1:20 to 18:1;

[0015] Further, the mass ratio of the compound of formula (I) to Feneptamidoquin is 1:20, 1:9, 1:3, 5:9, 9:5, 9:1, 18:1;

[0016] Further, the mass ratio of the compound of formula (I) to Feneptamidoquin is 1:9 to 9:1;

[0017] Further, the mass ratio of the compound of formula (I) to Feneptamidoquin is 1:9, 1:3, 5:9, 9:5, 9:1;

[0018] Further, the mass ratio of the compound of formula (I) to Fenopyramid is 1:24 to 20:1;

[0019] Further, the mass ratio of the compound of formula (I) to Fenopyramid is 1:24, 1:12, 1:5, 1:2, 3:1, 6:1, 20:1;

[0020] Further, the mass ratio of the compound of formula (I) to Fenopyramid is 1:12 to 3:1;

[0021] Further, the mass ratio of the compound of formula (I) to Fenopyramid is 1:12, 1:5, 1:2, or 3:1;

[0022] Further, based on the total weight of the bactericidal composition being 100 wt%, the sum of the contents of active ingredient A and active ingredient B in the bactericidal composition is 1 - 90 wt%;

[0023] Further, based on the total weight of the bactericidal composition being 100 wt%, the sum of the contents of active ingredient A and active ingredient B in the bactericidal composition is preferably 5 - 80 wt%;

[0024] Further, the bactericidal composition, in addition to the active ingredients, further contains agriculturally acceptable auxiliary ingredients, and the auxiliary ingredients are selected from one or more of wetting agents, dispersants, emulsifiers, thickeners, disintegrants, antifreezing agents, defoaming agents, solvents, preservatives, stabilizers, synergists, binders, or carriers;

[0025] Further, the wetting agent is selected from one or more of alkylbenzene sulfonates, alkylnaphthalene sulfonates, lignin sulfonates, sodium dodecyl sulfate, sodium dioctyl sulfosuccinate, α - olefin sulfonates, alkylphenol polyoxyethylene ethers, castor oil polyoxyethylene ethers, alkylphenol ethoxylates, fatty alcohol ethoxylates, sodium fatty alcohol polyoxyethylene ether sulfate, silkworm excrement, sophora powder, sapindus powder, SOPA, detergent, emulsifier 2000 series, and wetting penetrant F; and / or

[0026] Further, the dispersant is selected from one or more of lignin sulfonates, alkylnaphthalene sulfonate formaldehyde condensates, naphthalene sulfonates, triphenylvinylphenol ethoxylate phosphates, fatty alcohol ethoxylates, alkylphenol polyoxyethylene ethers, alkylphenol polyoxyethylene ether methyl ether condensates sulfates, fatty amine polyoxyethylene ethers, glycerol fatty acid ester polyoxyethylene ethers, polycarboxylates, polyacrylates, phosphates, EO - PO block copolymers, and EO - PO graft copolymers; and / or

[0027] Further, the emulsifier is selected from one or more of calcium dodecylbenzenesulfonate, alkylphenol formaldehyde resin polyoxyethylene ether, phenethylphenol polyoxyethylene polyoxypropylene ether, fatty alcohol ethylene oxide - propylene oxide copolymer, styrylphenol polyoxyethylene ether, castor oil polyoxyethylene ether, and alkylphenol ether phosphate; and / or

[0028] Further, the thickener is selected from one or more of xanthan gum, organic bentonite, gum arabic, sodium alginate, magnesium aluminum silicate, carboxymethyl cellulose, and silica white; and / or

[0029] Further, the disintegrant is selected from one or more of sodium sulfate, ammonium sulfate, aluminum chloride, sodium chloride, ammonium chloride, bentonite, glucose, sucrose, starch, cellulose, urea, sodium carbonate, sodium bicarbonate, citric acid, and tartaric acid; and / or

[0030] Further, the antifreeze is selected from one or more of alcohols, alcohol ethers, chlorinated hydrocarbons, and inorganic salts; and / or

[0031] Further, the defoamer is selected from one or more of C 10 -C 20 saturated fatty acid compounds, silicone oils, silicone compounds, C8-C 10 fatty alcohols; and / or

[0032] Further, the solvent is selected from one or more of benzene, toluene, xylene, durene, methanol, ethanol, isopropanol, n-butanol, dimethyl sulfoxide, dimethylformamide, cyclohexanone, alkylene carbonate, diesel oil, solvent oil, vegetable oils (such as soybean oil, corn oil, rapeseed oil, palm oil, etc.), vegetable oil derivatives, and water; and / or

[0033] Further, the preservative is selected from one or more of propionic acid, sodium propionate, sorbic acid, sodium sorbate, potassium sorbate, benzoic acid, sodium benzoate, sodium p-hydroxybenzoate, methyl p-hydroxybenzoate, Kathon, and 1,2-benzisothiazolin-3-one; and / or

[0034] Further, the stabilizer is selected from one or more of disodium hydrogen phosphate, oxalic acid, succinic acid, adipic acid, borax, 2,6-di-tert-butyl-p-cresol, triethanolamine oleate, epoxidized vegetable oil, kaolin, diatomaceous earth, bentonite, attapulgite, silica white, talc powder, montmorillonite, and starch; and / or

[0035] Further, the synergist is selected from synergistic phosphorus and synergistic ether; and / or

[0036] Further, the carrier is selected from one or more of ammonium salts, ground natural minerals, ground artificial minerals, silicates, resins, waxes, solid fertilizers, water, organic solvents, mineral oils, vegetable oils, and vegetable oil derivatives.

[0037] Further, the dosage form of the bactericidal composition is selected from solid preparations and / or liquid preparations and / or seed treatment preparations;

[0038] The solid preparations include powders, granules, balls, tablets, strips, wettable powders, oil-dispersed powders, milk powders, water-dispersible granules, milk granules, water-dispersible tablets, soluble powders, soluble tablets, or soluble granules;

[0039] The liquid preparations described above include solutions, sols, oils, film-forming oils, emulsifiable concentrates, latexes, dispersible liquids, pastes, water-in-oil emulsions, oil-in-water emulsions, microemulsions, fats, suspensions, microcapsule suspensions, oil suspensions, dispersible oil suspensions, suspoemulsions, microcapsule suspension-suspensions, microcapsule suspension-water-in-oil emulsions or microcapsule suspension-suspoemulsions;

[0040] Furthermore, the powder is a free-flowing powdery preparation suitable for dusting or spreading containing the active ingredient;

[0041] Furthermore, the granule is a free-flowing granular preparation containing the active ingredient with a certain particle size range;

[0042] Furthermore, the sphere is a spherical preparation containing the active ingredient (generally with a diameter greater than 6 mm);

[0043] Furthermore, the tablet is a sheet-like preparation containing the active ingredient with a certain shape and size (usually having two flat surfaces or convex surfaces, and the distance between the two surfaces is less than the diameter);

[0044] Furthermore, the strip is a strip-shaped or rod-shaped preparation containing the active ingredient (generally several centimeters in length, a few millimeters in width / diameter, that is, the length is greater than the diameter / width);

[0045] Furthermore, the wettable powder is a powdery preparation in which the active ingredient is dispersed into a suspension in water;

[0046] Furthermore, the oil-dispersible powder is a powdery preparation in which the active ingredient is dispersed into a suspension in an organic solvent;

[0047] Furthermore, the milk powder is a powdery preparation in which the active ingredient is dissolved in an organic solvent, wrapped in a soluble or insoluble inert ingredient, and dispersed in water to form an oil-in-water emulsion;

[0048] Furthermore, the water-dispersible granule is a granular preparation that disintegrates in water and the active ingredient is dispersed into a suspension;

[0049] Furthermore, the milk granule is a granular preparation in which the active ingredient is dissolved in an organic solvent, wrapped in a soluble or insoluble inert ingredient, and dispersed in water to form an oil-in-water emulsion;

[0050] Furthermore, the water-dispersible tablet is a sheet-like preparation that disintegrates in water and the active ingredient is dispersed into a suspension;

[0051] Furthermore, the soluble powder is a powdery preparation in which the active ingredient forms a true solution in water and may contain inert ingredients insoluble in water;

[0052] Furthermore, the soluble granule is a granular preparation in which the active ingredient forms a true solution in water and may contain inert ingredients insoluble in water;

[0053] Further, the soluble tablet is a tablet preparation in which the active ingredient forms a true solution in water and may contain inert ingredients insoluble in water;

[0054] Further, the soluble solution is a liquid preparation containing the active ingredient diluted with water to be transparent or semi-transparent and may contain inert ingredients insoluble in water;

[0055] Further, the sol is a colloidal preparation in which the active ingredient forms a true solution diluted with water;

[0056] Further, the oil preparation is a homogeneous liquid preparation containing the active ingredient diluted (or undiluted) with an organic solvent;

[0057] Further, the spreading oil preparation is an oil preparation that automatically spreads into an oil film containing the active ingredient on the water surface;

[0058] Further, the emulsifiable concentrate is a homogeneous liquid preparation in which the active ingredient is diluted and dispersed with water to form an emulsion;

[0059] Further, the latex is a latex preparation in which the active ingredient is diluted and dispersed with water to form an emulsion;

[0060] Further, the dispersible solution is a homogeneous liquid preparation in which the active ingredient is diluted and dispersed with water to form a suspension;

[0061] Further, the paste is a water-based paste preparation containing the active ingredient that can form a film and is generally used directly;

[0062] Further, the water emulsion is a liquid preparation in which the active ingredient (or its organic solution) forms an emulsion in water;

[0063] Further, the oil emulsion is a liquid preparation in which the active ingredient (or its aqueous solution) forms an emulsion in oil;

[0064] Further, the microemulsion is a transparent or semi-transparent microemulsion liquid preparation in which the active ingredient is in water and is used directly or after dilution with water;

[0065] Further, the fat preparation is an oily or fatty-based viscous preparation containing the active ingredient and is generally used directly;

[0066] Further, the suspending agent is a stable suspension liquid preparation in which the active ingredient is dispersed in water as solid fine particles and is generally used after dilution with water;

[0067] Further, the microcapsule suspending agent is a stable suspension liquid preparation in which microcapsules containing the active ingredient are dispersed in a liquid;

[0068] Further, the oil-based suspension concentrate is a stable suspension liquid preparation in which the active ingredient is dispersed as solid particles in a liquid, and it is generally diluted with an organic solvent for use;

[0069] Further, the dispersible oil-based suspension concentrate is a stable suspension liquid preparation in which the active ingredient is dispersed as solid particles in a non-aqueous medium, and it is generally diluted with water for use;

[0070] Further, the suspension emulsion is a heterogeneous liquid preparation in which the active ingredient is stably dispersed as solid particles and tiny water-insoluble droplets in a continuous aqueous phase;

[0071] Further, the microcapsule suspension-suspension concentrate is a stable suspension liquid preparation in which the active ingredient is dispersed as microcapsules and solid particles in water;

[0072] Further, the microcapsule suspension-emulsion in water is a heterogeneous liquid preparation in which the active ingredient is stably dispersed as microcapsules and tiny droplets in a continuous aqueous phase;

[0073] Further, the microcapsule suspension-suspension emulsion is a heterogeneous liquid preparation in which the active ingredient is stably dispersed as microcapsules, solid particles and tiny droplets in a continuous aqueous phase;

[0074] Further, the bactericidal composition can be prepared into a pesticide-acceptable formulation dosage form, and the formulation dosage form is a microemulsion, an emulsion in water, a suspension concentrate, a dispersible oil-based suspension concentrate, a soluble concentrate, an emulsifiable concentrate, a suspension emulsion, a microcapsule suspension, a water dispersible granule, a wettable powder, a granule, a seed treatment suspension concentrate, a seed treatment dry powder;

[0075] Further, the formulation dosage form is a microemulsion, an emulsifiable concentrate, a suspension concentrate, an emulsion in water, a water dispersible granule, a wettable powder, a seed treatment suspension concentrate.

[0076] The present invention also discloses the application of a bactericidal composition as described above in the prevention and control of plant diseases;

[0077] Further, the plant diseases are plant diseases caused by fungi or bacteria;

[0078] Further, the plant diseases are plant diseases caused by fungi;

[0079] Further, the plant diseases caused by fungi are wheat leaf rust;

[0080] Wheat leaf rust is a major disease during the growth of wheat, characterized by a wide distribution range, rapid spread, and great harm. It is one of the main factors leading to wheat yield loss, quality reduction, and low efficiency. Wheat leaf rust is distributed throughout China, and the degree of occurrence shows an increasing trend year by year.

[0081] The present invention has the following beneficial effects:

[0082] 1) The bactericidal composition of the present invention increases the bactericidal activity, has a synergistic effect on the target at a certain mass ratio, and has the effects of reducing the amount of pesticide used, reducing the use cost and reducing the environmental load;

[0083] 2) The bactericidal composition of the present invention is safe and efficient, safe for crops, non-target organisms, beneficial organisms and natural enemies, and has the effect of increasing production and ensuring harvest. Specific embodiments

[0084] In order to make the technical solutions, objectives and advantages of the present invention clearer, the present invention is described by the following specific embodiments, but the present invention can be implemented in various forms and should not be limited by the embodiments described herein.

[0085] Indoor bioassay of wheat leaf rust

[0086] Test basis: Refer to the Pesticide Indoor Bioassay Test Guidelines Fungicides Part 15: Test Pot Method for Controlling Wheat Leaf Rust NY / T 1156.15-2008 of the Agricultural Industry Standard of the People's Republic of China.

[0087] Test crops: Select the wheat variety Luyuan 502 susceptible to leaf rust for potting, sow 20 seeds in each pot, select 10 of them after emergence, grow to the 2-leaf 1-heart stage, and number them for standby.

[0088] Test target: Wheat leaf rust fungus (Puccinia recondita Rob.ex Desm.f.sp.tritici Erikss.et Henn.), and the strain is taken from Shenyang Sinochem Pesticide & Chemicals R & D Co., Ltd.

[0089] Preparation of spore suspension: Wash the fresh uredospores of rust fungus produced within 24 hours on the diseased leaves with 0.1% Tween-80 aqueous solution, and filter with 2 to 4 layers of gauze to prepare a suspension with a concentration of 1×10 5 spores / mL for standby.

[0090] Preparation of medicament: Dissolve the test medicament with acetone, and then dilute it with 0.1% Tween 80 aqueous solution. Different ratios are designed according to the purpose of mixing and the activity of the medicament. Each single agent and each mixed agent of each ratio are prepared into the required series of mass concentrations according to the equal ratio method.

[0091] Medicament treatment: Spray the liquid medicine evenly on the leaf surface until it is completely wet, and wait for the liquid medicine to dry naturally before standby. A treatment without medicament is set as a blank control. Each concentration treatment is repeated 4 times, and each repetition has 1 pot.

[0092] Inoculation and Cultivation: Spray inoculation was carried out with a spore suspension. After inoculation of the wheat seedlings, they were cultured in the dark with humidity maintained at 20 °C for more than 12 h. The optimum temperature during the humidity maintenance stage was 15 - 20 °C, and then they were cultured under the conditions of a temperature of 18 - 22 °C and a light ratio of L:D = 12:12 h.

[0093] Data Investigation: When the incidence rate of the blank control reached over 80%, the disease incidence of each treatment was investigated by grading.

[0094] The grading method was as follows:

[0095] Grade 0: No spore heaps;

[0096] Grade 1: The spore accumulation accounted for less than 5% of the entire leaf area;

[0097] Grade 3: The spore heaps accounted for 5% - 10% of the entire leaf area;

[0098] Grade 5: The spore heaps accounted for 10% - 25% of the entire leaf area;

[0099] Grade 7: The spore heaps accounted for 25% - 50% of the entire leaf area;

[0100] Grade 9: The spore heaps accounted for more than 50% of the entire leaf area.

[0101] Data Statistics and Analysis:

[0102] The disease index was calculated according to the following formula.

[0103]

[0104] In the formula:

[0105] X - disease index;

[0106] N i - number of diseased leaves at each level;

[0107] i - relative grade value;

[0108] N - total number of leaves investigated.

[0109] The control effect was calculated according to the following formula.

[0110]

[0111] In the formula:

[0112] P - control effect, unit: %;

[0113] CK - disease index of the blank control;

[0114] PT - disease index of the medicament treatment.

[0115] Referring to the standard bioassay method, the synergistic effect of the mixed agents was evaluated according to the co-toxicity coefficient method (CTC) of Sun Yunpei, that is, CTC ≤ 80 indicates antagonistic effect, 80 < CTC < 120 indicates additive effect, and CTC ≥ 120 indicates synergistic effect.

[0116] The co-toxicity coefficient (CTC) is calculated by the following formula.

[0117]

[0118] Where:

[0119] ATI—the measured toxicity index of the mixture;

[0120] S—the EC of the standard agent 50 , in milligrams per liter (mg / L);

[0121] M—the EC of the mixture 50 , in milligrams per liter (mg / L).

[0122] TTI = TI A ×P A +TI B ×P B

[0123] Where:

[0124] TTI—the theoretical toxicity index of the mixture;

[0125] TI A —the toxicity index of agent A;

[0126] P A —the percentage content of agent A in the mixture, in percentage (%);

[0127] TI B —the toxicity index of agent B;

[0128] P B —the percentage content of agent B in the mixture, in percentage (%).

[0129]

[0130] Where:

[0131] CTC—the co-toxicity coefficient;

[0132] ATI—the measured toxicity index of the mixture;

[0133] TTI—the theoretical toxicity index of the mixture.

[0134] The DPS data processing software was used to calculate the test results, and the toxicity regression equations, EC 50 and 95% confidence limits of the single agent of the test agent and the mixed agents with different ratios were obtained respectively.

[0135] Indoor bioactivity test results:

[0136] Table 1 results show that the EC 50 of the compound of formula (I) against wheat leaf rust is 0.2407 mg / L, and the EC 50 of feneptamidoquin against wheat leaf rust is 0.4519 mg / L. The compound of formula (I) and feneptamidoquin showed synergistic effects in the range of mass ratio from 1:20 to 18:1. Among them, when the mass ratio was from 1:20 to 9:1, the co-toxicity coefficient > 150, and the synergistic effect was significant. The co-toxicity coefficient value of the compound of formula (I) and feneptamidoquin = 5:9 was the largest, which was 296.362, and the EC 50 was 0.1161 mg / L.

[0137] Table 1 Determination results of synergistic effects of different ratios of the compound of formula (I) and feneptamidoquin on wheat leaf rust

[0138]

[0139] Table 2 results show that the EC 50 of fenopyramid against wheat leaf rust is 0.6061 mg / L. In the range of mass ratio from 1:36 to 20:1 of the compound of formula (I) and fenopyramid, the co-toxicity coefficient > 80, showing additive or synergistic effects; when the mass ratio was from 1:24 to 20:1, the co-toxicity coefficient > 120, showing synergistic effects. The co-toxicity coefficient value of the compound of formula (I) and fenopyramid = 1:2 was the largest, which was 212.040, and the EC 50 was 0.1905 mg / L, and the synergistic effect was significant.

[0140] Table 2 Determination results of synergistic effects of different ratios of the compound of formula (I) and fenopyramid on wheat leaf rust

[0141]

[0142]

[0143] Formulation examples

[0144] Preparation example 1:

[0145] 42% Wettable Powder of Compound (I)·Feneptamidoquin (15:27)

[0146] Formulation: 15% of Compound (I), 27% of Feneptamidoquin, 5% of sodium polycarboxylate, 5% of dispersant NNO, 5% of polyoxyethylene ether (NV1420), 3.5% of sodium dodecyl sulfate, 5% of bentonite, and the balance made up with kaolin;

[0147] Preparation method: Mix the active ingredient, other functional auxiliaries and fillers according to the formulation ratio, stir evenly in a stirring kettle, and pulverize and mix evenly for multiple times with an air flow pulverizer to obtain the wettable powder of the composition of the present invention.

[0148] Preparation Example 2:

[0149] 22% Suspension Concentrate of Compound (I)·Feneptamidoquin (8:14)

[0150] Formulation: 8% of Compound (I), 14% of Feneptamidoquin, 4% of polyether, 3% of phenethylphenol polyether phosphate salt, 3% of calcium sulfosuccinate (EP60P), 1.5% of sodium polycarboxylate, 1% of magnesium aluminum silicate, 0.25% of xanthan gum, 4.5% of ethylene glycol, 0.02% of benzisothiazolinone, 0.5% of silicone defoamer, and the balance made up with deionized water;

[0151] Preparation method: According to the formulation ratio, place the active ingredient, surfactant and other functional auxiliaries into a reaction kettle in sequence, add water and mix evenly, and obtain the suspension concentrate through high-speed shearing, wet grinding and finally homogenizing and filtering.

[0152] Preparation Example 3:

[0153] 12% Emulsifiable Concentrate of Compound (I)·Feneptamidoquin (2:10)

[0154] Preparation formulation: 2% of Compound (I), 10% of Feneptamidoquin, 1.5% of BHT, 10% of propylene carbonate, 15% of DMF, 5% of cyclohexanone, 3.5% of calcium dodecylbenzenesulfonate, 10% of fatty alcohol polyoxyethylene ether, and the balance made up with methyl oleate;

[0155] Preparation method: Add the active ingredient to the cosolvent according to the formulation ratio of the example, and add the surfactant and other functional auxiliaries thereto, and stir and mix evenly in a stirring and mixing kettle to obtain the emulsifiable concentrate.

[0156] Preparation Example 4:

[0157] 24% Compound (I) · Fenopyramid Suspension Concentrate (8:16)

[0158] Formulation: 8% Compound (I), 16% Fenopyramid, 4% polyether, 3% phenethylphenol polyether phosphate salt, 3% sodium dioctyl sulfosuccinate, 1.5% sodium polycarboxylate, 1% magnesium aluminum silicate, 0.2% xanthan gum, 4.5% ethylene glycol, 0.02% benzisothiazolinone, 0.5% silicone defoamer, deionized water to make up the balance;

[0159] Preparation method: The same as Preparation Example 2.

[0160] Preparation Example 5:

[0161] 48% Compound (I) · Fenopyramid Water Dispersible Granules (16:32)

[0162] Formulation: 16% Compound (I), 32% Fenopyramid, 3.5% sodium dodecyl sulfate, 8% polynaphthalene formaldehyde sulfonate (270K), 1% fatty alcohol polyoxyethylene ether sodium sulfate, 2% sodium polycarboxylate BX, 6.5% sodium lignosulfonate, 7% attapulgite, kaolin to make up the balance;

[0163] Preparation method: According to the formulation ratio, add the active ingredients to the carrier, and add surfactants and other functional auxiliaries thereto, mix, add 10 - 25% water after airflow pulverization, and then obtain the water dispersible granule product through kneading, granulation, drying, and screening; or spray water, granulate, dry the pulverized powder in a fluidized bed granulator, and then screen to obtain the product.

[0164] Field Efficacy Test of Wheat Leaf Rust

[0165] Test basis: Refer to "Pesticide Field Efficacy Test Guidelines (I) Fungicides for Controlling Cereal Rusts (Leaf Rust, Stripe Rust, Stem Rust) (GB / T 17980.23 - 2000)";

[0166] Test location: Wheat field in Nanfan Village, Shouguang City, Shandong Province;

[0167] Test target: Wheat leaf rust pathogen (Puccinia recondita.f.sp.tritici.);

[0168] Test crop and variety: Wheat (Tanmai 98);

[0169] Test setup: Each plot is distributed according to a randomized block design, and a protection row is set around each plot. Each treatment has 4 replicates, and each plot is 20m 2 , and the plots are randomly arranged.

[0170] Test agents:

[0171] Table 3 Test Treatments and Dosages of Medicines

[0172] Agent <![CDATA[Dosage g a.i / hm 2 > 22% Compound (I)·Feneptamidoquin Suspension Concentrate (8:14) 75 24% Compound (I)·Fenopyramid Suspension Concentrate (8:16) 75 30% Compound (I) Suspension Concentrate 100 15% Feneptamidoquin Suspension Concentrate 200 20% Fenopyramid Suspension Concentrate 250 Water Control /

[0173] Test method: The medicine was applied using the conventional spraying method at the initial stage of wheat leaf rust (during the wheat filling stage), and the second application was made 7 days later. A total of two applications were made, evenly spraying on both the front and back of the leaves, with a field water consumption of 600 L / hm 2 .

[0174] Investigation and statistical method: The investigation was carried out 10 days after the last application. Five diagonal points were randomly selected in each plot, 20 plants were investigated at each point, and the top 3 leaves of each plant (including the flag leaf if there is one) were investigated. The disease spots on each leaf were graded according to the percentage of the leaf area occupied by the disease spots.

[0175] Grading method:

[0176] Grade 0: Disease-free;

[0177] Grade 1: The disease spot area accounts for less than 5% of the entire leaf area;

[0178] Grade 3: The disease spot area accounts for 6% - 25% of the entire leaf area;

[0179] Grade 5: The disease spot area accounts for 26% - 50% of the entire leaf area;

[0180] Grade 7: The disease spot area accounts for 51% - 75% of the entire leaf area;

[0181] Grade 9: The disease spot area accounts for more than 75% of the entire leaf area.

[0182] Calculation method of drug efficacy

[0183] The disease index and control efficacy were calculated using the following formula

[0184]

[0185] During the test period, it was observed that the wheat in each treatment plot grew well, and no phytotoxicity occurred in each treatment.

[0186] The plot yield was measured at the wheat harvest stage.

[0187] Test results:

[0188] As shown in Table 4, the mixture of the compound shown in (I) and Feneptamidoquin and Fenopyramid showed good control effects against wheat leaf rust. 10 days after the last application, the control effects of 22% compound (I)·Feneptamidoquin suspension concentrate (8:14) and 24% compound (I)·Fenopyramid suspension concentrate (8:16) against wheat leaf rust were 83.10% and 85.13% respectively, with excellent control effects, significantly higher than those of the single-agent controls.

[0189] Table 4 Results of field efficacy trials of different treatments against wheat leaf rust

[0190]

[0191]

[0192] Note: The disease index and control effect in the table are the averages of each replication; different lowercase letters in the same column indicate that there are statistically significant differences between groups (p < 0.05).

[0193] As shown in Table 5, 22% compound (I)·Feneptamidoquin suspension concentrate (8:14) and 24% compound (I)

[0194] ·Fenopyramid suspension concentrate (8:16) had obvious yield-increasing effects on wheat, with yield-increasing rates of 18.95% and 16.72% respectively.

[0195] Table 5 Effects of different treatments on wheat yield

[0196]

[0197] During the test process, no phytotoxicity and no impact on crop growth and development were recorded, indicating that the test agents were safe for crops within the recommended dosage. No impact on non-target organisms was found within the dosage range of the agents. No extreme weather conditions such as heavy rainfall and strong winds were recorded during the test period.

[0198] The results of the field efficacy plot trials showed that the compound shown in (I) and the mixtures of Feneptamidoquin and Fenopyramid showed high control effects in the control of wheat leaf rust. They not only reduced the disease index, had significant control effects, and had little impact on the yield, but the mixed preparations also showed obvious yield-increasing effects. In addition, according to the observations after application, the wheat growth in each treatment area was normal and no phytotoxicity occurred, indicating that each agent was safe for wheat growth at the dosage used in this test.

[0199] Through indoor toxicity determination and field trials, the composition obtained by compounding the compound shown by formula (I) of the present invention with either Feneptamidoquin or Fenopyramid shows very good control effects against wheat leaf rust and has broad application prospects. The bactericidal composition or its preparation obtained by compounding in the present invention has remarkable control effects and has the characteristics of high efficiency, broad spectrum, low residue, long effective period, strong systemic property, etc.; and no phytotoxicity to crops was found in the tests, indicating that under the condition of improved bactericidal synergistic effect of the obtained bactericidal composition or preparation, the production cost and use cost can be reduced and it is safe for crops.

[0200] Although the present invention has been described in detail with general descriptions and specific embodiments above, based on the present invention, some modifications or improvements can be made, which are obvious to those skilled in the art. Therefore, these modifications or improvements made without departing from the spirit of the present invention all fall within the scope of protection required by the present invention.

Claims

1. A bactericidal composition, characterized in that: It contains active ingredient A and active ingredient B, wherein the active ingredient A is a compound represented by formula (I), and the chemical structure is: The active ingredient B is any one of feneptamidoquin and fenopyramid.

2. The bactericidal composition according to claim 1, characterized in that The mass ratio of the active ingredient A to the active ingredient B is 1:36 to 20:

1.

3. The bactericidal composition according to claim 1, characterized in that The mass ratio of the active ingredient A to the active ingredient B is 1:24 to 20:

1.

4. The bactericidal composition according to claim 3, characterized in that The mass ratio of the compound of formula (I) to feneptamidoquin is 1:20 to 18:1; The mass ratio of the compound of formula (I) to Fenopyramid is 1:24 to 20:

1.

5. The bactericidal composition according to claim 1, characterized in that Based on the total weight of the bactericidal composition being 100 wt %, the sum of the contents of active ingredient A and active ingredient B in the bactericidal composition is 1 to 90 wt %; Preferably, based on the total weight of the bactericidal composition being 100 wt %, the sum of the contents of active ingredient A and active ingredient B in the bactericidal composition is 5 to 80 wt %.

6. The bactericidal composition according to claim 1, characterized in that In addition to the active ingredients, the fungicide composition also contains agriculturally permitted auxiliary ingredients, and the auxiliary ingredients are selected from one or more of wetting agents, dispersants, emulsifiers, thickeners, disintegrants, antifreeze agents, defoaming agents, solvents, preservatives, stabilizers, synergists, binders or carriers.

7. The bactericidal composition according to claim 6, characterized in that The dosage form of the bactericidal composition is selected from solid preparations and / or liquid preparations.

8. The bactericidal composition according to claim 7, characterized in that The fungicide composition can be prepared into a formulation acceptable to pesticides, and the formulation is a microemulsion, an aqueous emulsion, a suspension, a dispersible oil suspension, a soluble solution, an emulsifiable concentrate, a suspoemulsion, a microcapsule suspension, a water-dispersible granule, a wettable powder, a granule, a seed treatment suspension, or a seed treatment dry powder; Preferably, the formulation is in the form of a microemulsion, emulsifiable concentrate, suspension, aqueous emulsion, water dispersible granules, wettable powder, or seed treatment suspension.

9. Use of the fungicidal composition according to any one of claims 1 to 8 in preventing and controlling plant diseases.

10. The use according to claim 9, characterized in that: The plant disease is a plant disease caused by fungi or bacteria; Preferably, the plant disease is wheat leaf rust caused by fungi.

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