Binary insecticide composition and application thereof

Abstract

The invention discloses an insecticide synergistic composition containing a piperic acid derivative compound and application of the insecticide synergistic composition, the composition is composed of two active ingredients of a compound A and a compound B, the component B is selected from modoflaner, sulfiflumin, tenvermectin or butenyl spinosad, the compound A is selected from I-72, I-77, I-77, I-77, I-77, I-77, I-77, I-77, I-77, I-77, I-77, I-77, I-77, I-77, I-77, I-77, the composition has the following structure: the weight part ratio of the active component A to the active component B in the # imgabs0 # composition is (50: 1)-(1: 50). The composition disclosed by the invention has the advantages of obvious synergism, resistance delay and the like, and can be used for preventing and treating various pests of lepidoptera, thysanoptera, diptera, hemiptera and the like.

Description

Technical Field

[0001] The present invention belongs to the technical field of pesticide application, and particularly relates to a binary insecticide composition and its application. Background Art

[0002] Chemical agents are still an effective method for controlling pests and diseases in agricultural production. However, there are some limitations in using single-component insecticides. By mixing insecticides with different action mechanisms, the range of insecticidal activity can be broadened, and the control effect on specific pests can be increased. In addition, mixing insecticides can also improve the overall control effect because different insecticides have different modes of action and effects on pests. By mixing, the synergistic effects of these actions can be exerted, enhancing the control effect on pests. At the same time, mixing different insecticides can slow down the rate of pest resistance development and extend the effective use period of insecticides.

[0003] Modoflaner is another phthalic diamide insecticide developed by Mitsui Chemicals Agro, Inc., Japan. Its structure is similar to that of broflanilide and cyproflanilide developed in China. The difference is the introduction of iodine and fluoropyridine structures. At a concentration of 100 mg / L, the kill rate of modoflaner against Spodoptera litura, Plutella xylostella, and Laodelphax striatellus reaches over 70% (6 days). The kill rate of modoflaner against adult Ctenocephalides felis reaches 95% (48 h) with an exposure treatment at a dose of 0.04 μg / cm 2 or a feeding treatment at a dose of 0.0064 mg / L. The kill rate of modoflaner against nymphs of Amblyomma americanum, adult Dermacentor variabilis, and adult Rhipicephalus sanguineus reaches 90% (48 h) with an exposure treatment at a dose of 0.2 μg / cm 2 After an in vitro injection at a dose of 0.032 μg / individual, female adult Amblyomma microplus do not lay eggs or the eggs laid do not hatch after 7 days. It is speculated that the mechanism of action of modoflaner mainly also exerts its effect by allosterically regulating the γ-aminobutyric acid-gated chloride channel, similar to isoxazoline insecticides and acaricides such as milvuran and yumi milvuran.

[0004] The content disclosed in Chinese Patent Application No. CN112457288 A. This invention relates to a piperic acid derivative and its application. Among them, compound I-72 was found to have contact and stomach toxicity activities and exhibit good conduction characteristics. This compound can be used to control various pests such as Lepidoptera, Hemiptera, Thysanoptera, Coleoptera, Acarina, etc. This compound has a novel structure, a wide insecticidal spectrum, and high activity. Different from other insecticides, this compound is not likely to cause cross-resistance in pests, which makes it promising to be an environmentally friendly agent for controlling resistant pests.

[0005] To improve the usage efficiency and prevent the target pests from rapidly developing resistance due to the long-term use of the above single agent, the present inventors conducted in-depth research and found that the mixing of two agents within a certain proportion range has a significant synergistic effect, expands the control spectrum, is also conducive to reducing the usage amount of the agent, reducing the number of spraying times, and delaying the generation of target resistance. Through literature retrieval, the technical solution of the binary insecticide composition containing compound I-72 has not been specifically disclosed. Summary of the Invention

[0006] The object of the present invention is to provide a synergistic binary composition containing compound I-72 with insecticidal and acaricidal activities.

[0007] To achieve the above object, the technical solution adopted by the present invention is as follows:

[0008] A binary insecticide composition, the composition being active ingredient A and active ingredient B, wherein active ingredient A is selected from compound I-72, and active ingredient B is selected from modoflaner, sulfiflumin, avermectin or spinetoram; the weight ratio of the two active ingredients A and B is 50:1 to 1:50;

[0009] The compound I-72 has the following structure:

[0010]

[0011] Preferably, in the composition, active ingredient A is selected from compound I-72, and active ingredient B is selected from modoflaner, sulfiflumin, avermectin or spinetoram; the weight ratio between the two active ingredients A and B is 20:1 to 1:20.

[0012] More preferably, in the composition, active ingredient A is selected from compound I-72, and active ingredient B is selected from modoflaner, sulfiflumin, avermectin or spinetoram; the weight ratio of the two active ingredients A and B is 10:1 to 1:10.

[0013] An application of the described insecticide composition, the described insecticidal composition is used for controlling pests and sanitary pests on fruit trees, vegetables, ornamental plants, tea, cotton, cereal crops.

[0014] The described insecticidal composition is used for controlling Lepidoptera, Thysanoptera, Diptera, Hemiptera pests and sanitary pests on fruit trees, vegetables, ornamental plants, tea, cotton, cereal crops.

[0015] Indoor bioactivity assays showed that the binary insecticide composition of the present invention has a synergistic effect. For example, Compound I-72 and modoflaner showed a synergistic effect against Plutella xylostella in the range of 20:1 to 1:20; Compound I-72 and modoflaner had a synergistic effect against Chilo suppressalis in the ratio range of 20:1 to 1:20.

[0016] Compound I-72 and sulfiflumin showed a good synergistic effect against the target Tetranychus cinnabarinus of Tetranychidae in the range of 20:1 to 1:20, and Compound I-72 and sulfiflumin showed a synergistic effect against Chilo suppressalis in the range of 20:1 to 1:20.

[0017] The binary insecticidal and acaricidal composition of the present invention can especially control pests, mites or urban sanitation pests on ornamental plants, fruit trees, vegetables, cotton, cereal crops and tea; in particular, the insecticidal and acaricidal composition of the present invention has a good control effect on Panonychus citri, Eotetranychus kankitus, Phyllocoptruta oleivora, Tetranychus viennensis, Bryobia rubrioculus, Panonychus ulmi, Tetranychus urticae, Calacarus carinatus, Polyphagotarsonemus latus, Brevipalpus obovatus, Tetranychus cinnabarinus, Calacarus carinatus, Tetranychus truncatus, Tetranychus dunhuangensis, Tetranychus turkestani, Petrobia latens, Aceria jujubifolia, Oligonychus truncatus, mites of the genera Tetranychus and Eotetranychus that damage jujube trees, and mites on flowers, vegetables, etc.

[0018] In particular, it can control Lepidoptera pests occurring on vegetables, fruit trees and field crops, such as Chilo suppressalis, Tryporyza incertulas, Mythimna separata, Sesamia inferens, Diaphania indica, Etiella zinckenella, Agrotis ypsilon, Diatraea saccharalis, Ostrinia furnacalis, Athetis lepigone, Helicoverpa armigera, Spodoptera exigua, Spodoptera litura, Cnaphalocrocis medinalis, Mamestra brassicae, Hyphantria cunea, Geometridae, Carposina niponensis, Grapholita molesta, Leguminivora glycinivorella, Spodoptera frugiperda, Plutella xylostella, Pieris rapae, Helicoverpa assulta, Manduca sexta; Homoptera pests occurring on vegetables, fruit trees and field crops such as Bemisia tabaci, Trialeurodes vaporariorum, Sitobion avenae, Myzus persicae, Aphis gossypii, Aphis medicaginis, Aphis citricola, Laodelphax striatellus, Nilaparvata lugens; it can be used to control Thysanoptera pests that damage crops such as Frankliniella occidentalis, Thrips palmi, Thrips tabaci, Thrips oryzae, Thrips mymaridis, Thrips mangiferae.

[0019] Under the existing drug use situation, the composition of the present invention has the following advantages:

[0020] The composition has a significant synergistic effect, which improves the control effect on pests; since the compound I-72 has a novel mechanism of action and has no cross-resistance with existing pesticides, it can be used to control resistant pests. The application of the composition not only expands the insecticide spectrum of the compound I-72, but also delays the generation and development of pest resistance. The present invention can produce many additional advantages and effects by mixing the active component A with the pesticide, overcome the shortcomings of the single use of pesticides, improve the efficacy, slow down the generation of resistance, and control a variety of pests at the same time, which can achieve the characteristics of broad spectrum, high efficiency, delayed resistance, environmental protection, etc.; more importantly, through the reasonable mixing of pesticides, the service life of new varieties in the market can be extended, and the research and development costs of new pesticides can be reduced.

[0021] Specific implementation method of bioassay

[0022] The following examples are used to specifically illustrate the present invention so that it can be fully understood. The active component A of the present invention is prepared according to the description in the patent document CN112457288; the active component B in the composition is synthesized according to the prior art. The active components in the formula examples are metered and added after 100%, and each component is measured by weight percentage.

[0023] Biological Activity Assay Examples The following examples are used to illustrate the technical solutions of the present invention, but the present invention is not limited to the following examples.

[0024] Example 1 Indoor joint toxicity test of compound I-72 and modoflaner against Plutella xylostella

[0025] Test subjects: Diamondback moth (Plutella xylostella), 3rd instar larvae.

[0026] Test conditions: Temperature: 24-26°C, Humidity: RH 60%, Light: L:D=14:10

[0027] Preparation method: Use an electronic analytical balance to accurately weigh the test drugs, add appropriate amount of solvent to completely dissolve the two original drugs, prepare the mother solution of required concentration with water containing 0.1% Tween 80, mix the two active ingredient mother solutions in a certain proportion to prepare a mixed solution, and dilute them into a series of drug solutions with a certain concentration gradient according to the designed experimental dosage.

[0028] Experimental method: First, fresh cabbage leaves grown in the greenhouse were selected, and circular leaflets with a diameter of 3 cm were punched out of the cabbage leaves. According to the experimental design, the leaves were placed in the prepared solution and immersed for 10 seconds in the order from low dose to high dose. After natural drying, they were placed in a culture dish with a diameter of 9 cm and filter paper, and neatly arranged healthy test insects were inoculated. Ten insects were treated for each treatment, and each treatment was repeated 3 times. A blank control was also set up.

[0029] Place the processed test materials in the observation chamber, and the temperature, humidity, and light in the observation chamber can be adjusted as needed. After 48 hours, investigate the number of dead and live insects, calculate the corrected mortality rate using the Abbott formula, and perform statistical analysis using DPS data processing software to obtain the toxicity regression equations, LC 50 values and 95% confidence limits for each tested single agent and each different ratio mixture. Then, use the Sun y-p method to calculate the co-toxicity coefficient of each ratio to evaluate the mixing effect. The test results are shown in Table 1.

[0030] The co-toxicity coefficient (CTC value) of the mixture is calculated according to the following formula:

[0031]

[0032] In the formula: ATI - measured toxicity index of the mixture; S - LC 50 of the standard agent; M - LC 50 of the mixture.

[0033] TTI = TIA × PA + TIB × PB

[0034] In the formula: TTI - theoretical toxicity index of the mixture; TIA - toxicity index of agent A; PA - percentage content of agent A in the mixture; TIB - toxicity index of agent B; PB - percentage content of agent B in the mixture.

[0035]

[0036] In the formula: CTC - co-toxicity coefficient; ATI - measured toxicity index of the mixture; TTI - theoretical toxicity index of the mixture.

[0037] The co-toxicity coefficient (CTC) of the compound mixture: CTC ≥ 120 indicates synergistic effect; 80 < CTC < 120 indicates additive effect; CTC ≤ 80 indicates antagonistic effect.

[0038] The test results are shown in Table 1. It can be seen from the table that the mixture of compound I-72 and modoflaner shows a synergistic effect between 20:1 and 1:20.

[0039] Table 1 Indoor combined toxicity determination results of the composition containing compound I-72 against the 3rd instar larvae of Plutella xylostella

[0040]

[0041]

[0042] Example 2 Indoor combined toxicity determination test of the mixture of compound I-72 and modoflaner against Chilo suppressalis

[0043] Test target: Chilo suppressalis (Walker), 3rd instar larvae, sensitive strain reared indoors.

[0044] Preparation of liquid medicine: According to different test requirements, accurately weigh the test samples respectively with an electronic analytical balance. The technical material is dissolved in acetone, and then diluted with 0.1% Tween 80 water into a series of liquid medicines with a certain concentration gradient according to the test design dose.

[0045] Test method: First, select fresh rice seedlings, cut their stems into 5-cm segments, and immerse them in the prepared liquid medicine for 10 s in the order from low dose to high dose according to the test design. After natural air drying, place them in a 9-cm-diameter petri dish with filter paper, and introduce neat and healthy test insects, 10 for each treatment, with 3 replicates for each treatment. Additionally, set a blank control.

[0046] Place the treated test materials in the observation room, where the temperature, humidity, and light can be adjusted as needed. After 48 hours, investigate the number of dead and alive insects. The formula for calculating the drug efficacy and the method for evaluating the combined toxicity of the mixture are the same as in Example 1. The test results are shown in Table 2.

[0047] The test results are shown in Table 2. It can be seen from the table that the mixture of Compound I-72 and modoflaner has an obvious synergistic effect on Chilo suppressalis at the ratio of 20:1 to 1:20.

[0048] Table 2 Results of indoor combined toxicity determination of Compound I-72 and modoflaner against Chilo suppressalis

[0049]

[0050]

[0051] Example 3 Indoor combined toxicity determination test of Compound I-72 and sulfiflumin against Tetranychus cinnabarinus

[0052] Test object: Tetranychus cinnabarinus (Boisduval), adult mites.

[0053] Test conditions: Temperature: 24 - 26 °C, Humidity: RH 60%, Light: L:D = 14:10

[0054] Preparation method: Accurately weigh the test agents respectively with an electronic analytical balance. After completely dissolving the two technical materials in appropriate solvents, prepare the mother liquor with the required concentration with water containing 0.1% Tween 80. Mix the mother liquors of the two active ingredients in a certain proportion to prepare a mixed solution, and dilute it into a series of liquid medicines with a certain concentration gradient according to the test design dose.

[0055] Test method: The method of spraying on plants with pests was adopted. Taking kidney bean seedlings as carriers and treating with clear water as the blank control, the test was set with 3 replicates. Select kidney bean seedlings cultured in the greenhouse, transplant them into 7-cm paper cups when the first pair of true leaves unfolds, investigate the base number after inoculating female adult mites, then use an airbrush manual sprayer to evenly spray the prepared liquid medicine on both the front and back sides of the leaves, and move them into the observation room for cultivation and observation after natural air drying.

[0056] Place the treated test materials in the observation room, where the temperature, humidity, and light in the observation room can be adjusted as needed. Investigate the number of dead and live insects after 72 hours. The formula for calculating the drug efficacy and the method for evaluating the combined toxicity of the mixed agent are the same as in Example 1. The test results are shown in Table 3.

[0057] The test results are shown in Table 3. It can be seen from the table that the mixture of Compound I-72 and sulfiflumin shows a synergistic effect between 20:1 and 1:20.

[0058] Table 3 Results of indoor combined toxicity determination of the composition containing Compound I-72 against Tetranychus cinnabarinus

[0059]

[0060] Example 4 Indoor combined toxicity determination test of the mixture of Compound I-72 and sulfiflumin against Chilo suppressalis

[0061] Test target: Chilo suppressalis (Walker), 3rd instar larvae, sensitive strain reared indoors.

[0062] Preparation of liquid medicine: According to different test requirements, accurately weigh the test samples respectively with an electronic analytical balance. The technical materials are dissolved in acetone, and then diluted with 0.1% Tween 80 water into a series of liquid medicines with a certain concentration gradient according to the test design dose.

[0063] Test method: First, select fresh rice seedlings, cut their stems into 5-cm segments, and place them in the prepared liquid medicine in the order from low dose to high dose according to the test design for impregnation for 10 s. After natural air drying, place them in a 9-cm diameter petri dish with filter paper, and inoculate neat and healthy test insects, 10 for each treatment, with 3 replicates for each treatment, and set a blank control.

[0064] Place the treated test materials in the observation room, where the temperature, humidity, and light in the observation room can be adjusted as needed. Investigate the number of dead and live insects after 48 hours. The formula for calculating the drug efficacy and the method for evaluating the combined toxicity of the mixed agent are the same as in Example 1. The test results are shown in Table 4.

[0065] The test results are shown in Table 4. It can be seen from the table that the mixture of Compound I-72 and sulfiflumin has an obvious synergistic effect on Chilo suppressalis at the ratio of 20:1 to 1:20.

[0066] Table 4 Results of indoor combined toxicity determination of Compound I-72 mixed with sulfiflumin against Chilo suppressalis

[0067]

[0068] Example 5 Indoor Activity Determination of the Mixture of Compound I-72 and abamectin against Adult Bemisia tabaci

[0069] Test object: Bemisia tabaci (Gennadius), adults, sensitive strain reared indoors.

[0070] Test conditions: Temperature: 25-27°C, Humidity: RH 60%, Illumination: L:D = 14:10

[0071] Preparation method: Weigh the test agents accurately with an electronic analytical balance. After completely dissolving the two technical materials in an appropriate solvent respectively, prepare the stock solution with the required concentration with water containing 0.1% Tween 80. Mix the stock solutions of the two active ingredients in a certain proportion to prepare a mixed solution, and dilute it into a series of liquid medicines with a certain concentration gradient according to the test design dose.

[0072] Test method: Adopt the spraying method. Select the kidney bean seedlings cultivated in the greenhouse, spray evenly in the order from low dose to high dose according to the test design, air dry naturally and then cover with a self-made glass cover, introduce the test insects, 20-30 insects per treatment, with 3 replicates, and set a blank control. Investigate the number of dead and live insects after 72 hours. The formula for calculating the drug efficacy and the method for evaluating the combined toxicity of the mixture are the same as in Example 1.

[0073] The test results are shown in Table 5. It can be seen from the table that the mixture of Compound I-72 and abamectin has a synergistic effect on adult Bemisia tabaci at the ratio of 50:1 to 1:50.

[0074] Table 5 Results of indoor combined toxicity determination of Compound I-72 mixed with abamectin against Bemisia tabaci

[0075]

[0076] Example 6 Field Efficacy Example

[0077] The experiment was conducted in Xinkaihe Village, Longgang Township, Sujiatun District, Shenyang City, Liaoning Province. The test crop was tomato. The test samples were accurately weighed separately using an electronic analytical balance. The technical material was dissolved in acetone and then diluted with 0.1% Tween 80 water according to the experimental design to prepare a liquid medicine with a certain concentration. The concentrations of Compound I-72: abamectin (3:1), Compound I-72: abamectin (1:1), Compound I-72: abamectin (1:3), Compound I-72, and abamectin were all 100 mg / L. Another treatment with clear water was set as the blank control. There were a total of 6 treatments, with 3 replicates for each treatment, arranged in a randomized block design. The population density of pests was investigated before application, and then investigated once at 2 days and 7 days after application respectively.

[0078] Calculation method of efficacy:

[0079] Reduction rate of pest population (%) = (number of pests before application - number of pests after application) / number of pests before application × 100

[0080] Control effect (%) = (reduction rate of pest population in treatment area - reduction rate of pest population in control area) / (100 - reduction rate of pest population in control area) × 100

[0081] The test results are shown in Table 6. It can be seen from the table that the mixture of Compound I-72 and abamectin has better fast-acting insecticidal properties compared with each single agent, and its long-lasting effect is better than that of each single agent.

[0082] Table 6 Field test results of the mixture of Compound I-72 and abamectin against Bemisia tabaci

[0083]

[0084] Note: Lowercase letters represent significant differences at the 0.05 level

[0085] The insecticidal and acaricidal composition provided by the present invention has the advantages of high potency, broad spectrum, safety, and environmental protection. It can reduce the amount of pesticide used and residues, and prevent pests from developing drug resistance. It is applicable to a variety of crops and pests and has broad application prospects. The researchers have carried out improvements and modifications on it. The improvements and modifications belong to the protection scope of the present invention.

Claims

1. A binary insecticide composition, characterized in that: The composition comprises active ingredient A and active ingredient B, wherein active ingredient A is selected from compound I-72, and active ingredient B is selected from modoflaner, sulfiflumin, avermectin or spinetoram; the weight ratio of the two active ingredients A and B is 50:1 to 1:50; Compound I-72 has the following structure:

2. The pesticidal composition according to claim 1, characterized in that: In the composition, active ingredient A is selected from compound I-72, and active ingredient B is selected from modoflaner, sulfiflumin, avermectin or spinetoram; the weight ratio between the two active ingredients A and B is 20:1 to 1:

20.

3. The pesticidal composition according to claim 2, wherein: In the composition, active ingredient A is selected from compound I-72, and active ingredient B is selected from modoflaner, sulfiflumin, avermectin or spinetoram; the weight ratio of the two active ingredients A and B is 10:1 to 1:

10.

4. Use of the insecticide composition according to claims 1 to 3, characterized in that: Application of the insecticidal composition in controlling pests on fruit trees, vegetables, ornamental plants, tea, cotton, cereal crops and sanitary pests.

5. The application according to claim 4, characterized in that: Application of the insecticidal composition in controlling Lepidoptera, Thysanoptera, Diptera, Hemiptera pests on fruit trees, vegetables, ornamental plants, tea, cotton, cereal crops and sanitary pests.

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