Balancing oil, balancing grease powder and application thereof

Abstract

The invention belongs to the field of feed, and discloses balance oil which comprises the following components in percentage by weight: 15-20% of fish oil, 20-30% of soybean oil, 5-10% of linseed oil, 20-30% of lysophospholipid, 5-10% of coconut oil, 15-20% of corn oil and 1-5% of red palm oil. The balance oil is applied to pregnant sows, and experimental verification shows that the balance oil can effectively improve the reproductive performance of the sows and the growth performance of piglets. Meanwhile, the invention further discloses balancing grease powder and application thereof.

Description

Technical Field

[0001] The present invention relates to the field of feeds, and particularly to a balanced oil, a balanced oil powder and their uses. Background Art

[0002] A large number of studies have shown that adding oils and fats to the diets of sows during pregnancy and lactation can significantly improve reproductive and lactation performance, reduce the weight and backfat loss of sows during lactation, and increase the survival rate, birth weight and weaning weight of newborn piglets. Supplementing oils and fats in the diets of sows can promote the secretion of follicle-stimulating hormone (FSH) and luteinizing hormone (LH), and significantly affect the progesterone synthesis of corpora lutea after ovulation, promoting follicle development and ovulation, thereby improving reproductive performance. In addition, adding coconut oil and phospholipid oil powder to the diets of sows can increase the birth weight of piglets, and adding fish oil and linseed oil can increase the number of weaned piglets per litter and the weaning weight of piglets per litter.

[0003] There are many types of fatty acids in oils and fats. According to the carbon chain length, they can be divided into short-chain fatty acids, medium-chain fatty acids and long-chain fatty acids. Fatty acids with different structures, such as short-chain fatty acids, medium-chain fatty acids and long-chain n-3 polyunsaturated fatty acids, etc., play important roles in the reproduction and lactation of sows and the growth and development of piglets, and the roles played are different. Therefore, rationally formulating oils and fats with different fatty acid compositions can not only effectively save oil and fat resources, but also make up for the deficiencies in the fatty acid composition of single oils and fats. However, at present, single oils and fats are more commonly used in the feed industry, and single oils and fats cannot meet the needs of the reproductive performance of sows. Summary of the Invention

[0004] The purpose of the present invention is to provide a balanced oil. The balanced oil of the present invention is applied to pregnant sows. Through experimental verification, it is found that it can effectively improve the reproductive performance of sows and the growth performance of piglets.

[0005] At the same time, the present invention also discloses a balanced oil powder and its uses.

[0006] The specific scheme of the present invention is as follows:

[0007] A balanced oil, comprising the following components in weight percentage:

[0008] Fish oil 15-20wt%, soybean oil 20-30%, linseed oil 5-10%, lecithin 20-30%, coconut oil 5-10%, corn oil 15-20%, red palm oil 1-5%.

[0009] A balanced oil powder, comprising a carrier and the above-mentioned balanced oil.

[0010] In the above-mentioned balanced oil powder, the carrier is expanded corn or expanded soybean meal.

[0011] In the above-mentioned balanced oil powder, the weight ratio of the carrier to the balanced oil is 1:1.

[0012] Meanwhile, the present invention also discloses the use of the above-mentioned balanced oil or any one of the above-mentioned balanced oil powders in preparing a feed additive for sows.

[0013] In the above-mentioned use, the feed additive for sows is used to reduce the contents of cholesterol, triglyceride, high-density lipoprotein and uric acid in the plasma of sows after feeding the sows, and / or to increase the contents of milk protein and non-fat solids in the milk of sows.

[0014] In the above-mentioned use, the feed additive for sows is used to increase the number of weaned piglets, the survival rate of weaned piglets and the milk yield, increase the weaning litter weight and the weaning litter weight gain, and reduce the parturition process after feeding the sows.

[0015] In the above-mentioned use, the feed additive for sows is used to improve the intestinal health of piglets after feeding the sows.

[0016] In the above-mentioned use, the sow is a pregnant sow.

[0017] In the above-mentioned use, the balanced oil is applied to sows from gestation to lactation.

[0018] The beneficial effects of the present application are as follows:

[0019] In the research of the present application, it is found that feeding a diet containing 2% balanced oil powder to pregnant / lactating sows can improve the reproductive performance of sows and the growth performance of piglets. Adding balanced oil powder to the diet of pregnant / lactating sows can alleviate the damage to the intestinal structure and function of piglets caused by LPS, promote the mitochondrial fusion of intestinal epithelial cells, and inhibit the apoptosis of intestinal epithelial cells. Description of the Drawings

[0020] Figure 1A Chart showing the effect of adding balanced oil powder to the diet of sows on the backfat at parturition;

[0021] Figure 1B Chart showing the effect of adding balanced oil powder to the diet of sows on the backfat at weaning;

[0022] Figure 1C Chart showing the effect of adding balanced oil powder to the diet of sows on the backfat loss;

[0023] Figure 2A Chart showing the effect of adding balanced oil powder to the diet of sows on the composition of colostrum;

[0024] Figure 2B Chart showing the effect of adding balanced oil powder to the diet of sows on the immunoglobulin IgA in milk;

[0025] Figure 2C Chart of the effect of adding balanced oil powder to the diet of sows on immunoglobulin IgM in milk

[0026] Figure 3A Photos of jejunal villi of piglets in different experimental groups

[0027] Figure 3B Chart of the effect of adding balanced oil powder to the diet of sows on the jejunal morphology of piglets after LPS stimulation

[0028] Figure 4 Chart of the effect of adding balanced oil powder to the diet of sows on the intestinal repair function of piglets after LPS stimulation

[0029] Figure 5 Chart of the effect of adding balanced oil powder to the diet of sows on the intestinal digestive function of piglets after LPS stimulation

[0030] Figure 6A Electrophoretogram of tight junction proteins in the intestine of piglets after LPS stimulation with balanced oil powder added to the diet of sows

[0031] Figure 6B Chart of the effect of adding balanced oil powder to the diet of sows on the intestinal barrier function of piglets after LPS stimulation

[0032] Figure 7 Chart of the effect of adding balanced oil powder to the diet of sows on the inflammatory factors in the jejunum of piglets after LPS stimulation

[0033] Figure 8A Chart of the effect of adding balanced oil powder to the diet of sows on the mRNA expression levels of Mfn1 and Mfn2 in the jejunal mucosa of piglets after LPS stimulation

[0034] Figure 8B Electrophoretogram of mRNA of Mfn1 and Mfn2 in the jejunal mucosa of piglets after LPS stimulation with balanced oil powder added to the diet of sows

[0035] Figure 8C Chart of the effect of adding balanced oil powder to the diet of sows on inhibiting the protein expression levels of Mfn1, Mfn2 and Opa1 in the jejunal mucosa of piglets caused by LPS stimulation

[0036] Figure 9 Chart of the effect of adding balanced oil powder to the diet of sows on the related indicators of intestinal cell apoptosis in piglets after LPS stimulation Detailed implementation method

[0037] The embodiments of the present invention will be described clearly and completely below. In the description of the present invention, it should be noted that for those conditions not specified in the embodiments, they are carried out according to the conventional conditions or the conditions recommended by the manufacturer. For the reagents or instruments whose manufacturers are not specified, they are all conventional products that can be obtained by purchasing in the market.

[0038] Effect of the first part of balanced oil powder on the reproductive performance of sows and the growth performance of piglets

[0039] This experiment studied the effects of balanced oil powder on the reproductive performance of sows and the growth performance of piglets. A total of 24 healthy sows at 90 days of gestation with similar expected delivery dates and parities were selected and randomly divided into two groups, with 12 sows in each group, namely: ① Control group, fed a diet containing 2% soybean oil; ② Balanced oil powder group, fed a diet containing 2% balanced oil powder. The experiment started from the 90th day of sow gestation and ended at 21 days after the piglets were weaned. The results showed that: (1) Feeding the diet containing 2% balanced oil powder significantly increased the milk production of sows, the number of weaned piglets and the weaning survival rate of piglets (P<0.05), and there was a tendency to increase the litter weight at weaning, litter weight gain at weaning and daily weight gain of piglets, and to reduce the parturition duration of sows (P<0.1), but had no significant effect on the number of piglets born per litter, the live piglet rate and the backfat loss of sows. (2) Feeding the diet containing 2% balanced oil powder significantly reduced the contents of total cholesterol, triglyceride, high-density lipoprotein and uric acid in the plasma of sows (P<0.05). (3) Feeding the diet containing 2% balanced oil powder significantly increased the contents of milk protein, non-fat solids, IgA and IgM in the colostrum of sows (P<0.05), but had no significant effect on the milk fat content. The above results indicate that feeding the diet containing 2% balanced oil powder to pregnant / lactating sows can improve the reproductive performance of sows and the growth performance of piglets.

[0040] 1 Materials and methods

[0041] 1.1 Experimental materials

[0042] The pregnant sows used in the experiment were raised in Nanpingshan Pig Farm, Jingshan City, Jingmen City, Hubei Province. The diet formula of the sows is shown in Table 1.

[0043] The soybean oil was provided by Yihai Kerry Golden Arowana Grain and Oil Food Co., Ltd. The fatty acid ratio of the soybean oil is shown in Table 2.

[0044] The main components of the balanced oil powder are balanced oil and expanded corn flour, and the weight ratio of the two is 1:1; the components of the balanced oil are: fish oil 15.5wt%, soybean oil 25.3%, linseed oil 7.8%, lysophospholipid 22.0%, coconut oil 8.8%, corn oil 17.5%, red palm oil 3.1%. The fatty acid composition is shown in Table 2.

[0045] Table 1 Sow diet formula table

[0046]

[0047]

[0048] Table 2 Fatty acid composition of soybean oil and balanced oil powder

[0049]

[0050]

[0051] 1.2 Feeding management

[0052] The experiment started from the 90th day of pregnancy of sows and ended at the weaning of piglets at 21 days old. The pregnant sows were fed restrictedly, and the lactating sows were fed ad libitum. All sows had free access to water. The pregnant and lactating sows were fed individually in the confined stalls.

[0053] 1.3 Experimental design

[0054] A total of 24 pregnant sows at 90 days of pregnancy with similar expected delivery dates, parities and health conditions were selected for the experiment. They were randomly divided into two groups, with 12 sows in each group, namely: ① Control group (Control, Con), fed a diet containing 2% soybean oil; ② Balanced oil powder group (Balanced oil powder, Bop), fed a diet containing 2% balanced oil powder. The experiment started from the 90th day of pregnancy of sows and ended at 21 days after the birth of piglets.

[0055] 1.4 Sample collection and processing

[0056] On the day of farrowing, 10 mL of blood was collected from the marginal ear vein of sows. After natural coagulation, it was centrifuged at 3000 r / min at 4 °C for 10 min, and the separated serum was stored at -20 °C. After the sows gave birth to 5 piglets, 10 mL of colostrum from the 4th or 5th pair of mammary glands of the sows was collected and stored at -20 °C. The pre-caval vein blood of nursing piglets was collected at 21 days old, 5 mL each. After natural coagulation, it was centrifuged at 3000 r / min at 4 °C for 10 min, and the separated serum was stored at -20 °C.

[0057] 1.5 Detection indexes and determination methods

[0058] 1.5.1 Reproductive performance of sows

[0059] Taking the replicates as units, after the sows completed farrowing, the number of piglets born per litter, the number of live piglets, the live piglet rate and the number of piglets nursed by sows, the number of weaned piglets, the weaned piglet survival rate, the parturition process, the daily feed intake of sows during lactation, the milk yield, the individual weight of newborn piglets, the individual weight of weaned piglets, the weight of newborn litter, the weight of weaned litter, the weight gain of weaned litter and the daily weight gain of piglets were counted.

[0060] Litter size (number / litter): The total number of piglets born in the same litter from the start to the end of farrowing of the sow.

[0061] Number of live piglets (number / litter): The total number of live piglets born in the same litter from the start to the end of farrowing of the sow.

[0062] Live piglet rate (%): The total number of live piglets born in the same litter from the start to the end of farrowing of the sow / The total number of piglets born in the same litter from the start to the end of farrowing of the sow.

[0063] Number of piglets nursed by the sow (number): The number of piglets nursed by each sow.

[0064] Number of weaned piglets (number / litter): The number of piglets that survive at weaning from the litter born by the sow.

[0065] Survival rate of weaned piglets (%): The proportion of piglets that survive at weaning among the piglets born by the sow.

[0066] Duration of farrowing (hours): The length of time from the start of farrowing of the sow to the completion of the birth of all piglets.

[0067] Daily feed intake during lactation of sow (kg): The amount of feed ingested by the sow per day during lactation.

[0068] Milk production (kg / day): The average daily milk production of the sow during the lactation stage.

[0069] Individual birth weight of piglets (kg): The weight of piglets on the day of birth.

[0070] Individual weaning weight of piglets (kg): The weight of piglets on the day of weaning.

[0071] Litter birth weight (kg): The sum of the weights of all piglets in the same litter on the day of birth.

[0072] Litter weaning weight (kg): The sum of the weights of all piglets in the same litter on the day of weaning.

[0073] Litter weaning weight gain (kg): The difference between the litter birth weight and the litter weaning weight.

[0074] Daily weight gain of piglets (g): The ratio of the weight gain of piglets during weaning to the number of days of weaning.

[0075] 1.5.2 Backfat of sows

[0076] Measure the backfat thickness of sows at the time of entering the trial, farrowing, and weaning. When measuring, take a position 6 - 8 cm from the dorsal midline at the last rib. After removing the hair, apply B-ultrasound oil or vegetable oil and attach the probe to the measurement site. Read the value after the backfat meter reading stabilizes.

[0077] 1.5.3 Serum biochemical indexes

[0078] Total protein, albumin, glucose, triglyceride, total cholesterol, calcium, phosphorus, aspartate aminotransferase, alanine aminotransferase, uric acid, creatinine, urea nitrogen, etc. were measured using a fully automatic plasma biochemical analyzer.

[0079] 1.5.4 Composition and Immune Index of Sow Milk

[0080] Composition of sow colostrum: The contents of lactose, milk fat, milk protein and non-fat solids in colostrum were detected using a milk composition analyzer.

[0081] Content of sow colostrum immunoglobulins (IgG and IgA): Determined using an ELISA kit.

[0082] 1.6 Statistical Methods

[0083] All data of the experiment were analyzed by independent samples t-test using SPSS 22.0 statistical software. All data were expressed as mean and overall SEM. P < 0.05 represented significant difference, and P < 0.1 represented a significant trend.

[0084] 1.7 Experimental Results

[0085] 1.7.1 Effects of Adding Balanced Oil Powder to Sow Diets on Sow Reproductive Performance

[0086] As shown in Table 3, adding balanced oil powder to the diet could significantly increase the number of weaned piglets, the weaning survival rate of piglets and milk production (P < 0.05), and there was a tendency to increase the weaning litter weight (P = 0.073) and the weaning litter weight gain (P = 0.058), and a tendency to reduce the parturition duration (P = 0.051). There was no significant effect on the number of piglets born per litter, the number of live piglets and the daily feed intake during lactation.

[0087] Table 3 Effects of Adding Balanced Oil Powder to Sow Diets on Sow Reproductive Performance

[0088] Item Control group Balanced oil powder group SEM P value Number of piglets born per litter (heads / litter) 13.27 14.36 0.716 0.272 Number of live piglets (heads / litter) 13.00 13.64 0.620 0.512 Live piglet rate (%) 98.13 98.82 1.471 0.770 Number of piglets nursed by sows (heads) 11.27 11.91 0.388 0.283 Number of weaned piglets per litter (heads / litter) <![CDATA[9.82 b > <![CDATA[11.70 a > 0.350 0.001 Weaned piglet survival rate (%) <![CDATA[90.56 b > <![CDATA[98.96 a > 2.180 0.020 Duration of farrowing (hours) 4.35 3.57 0.269 0.051 Daily feed intake during lactation (kg) 4.14 4.19 0.199 0.816 Milk production (kg) <![CDATA[8.01 b > <![CDATA[10.17 a > 0.479 0.021 Individual birth weight of piglets (kg) 1.45 1.47 0.045 0.519 Individual weaning weight of piglets (kg) 5.57 5.98 0.176 0.209 Litter birth weight (kg) 18.20 19.97 0.679 0.226 Weaned litter weight (kg) 57.23 69.81 3.190 0.073 Weaned litter weight gain (kg) 42.06 52.02 2.640 0.058 Daily weight gain of piglets (g) 191 216 6.1 0.074

[0089] Milk production = average daily gain of piglets (g / day) × 4 × number of weaned piglets per litter × lactation days / (lactation days × 1000)

[0090] Note: With different letters ab Indicates significant difference between treatment groups (P < 0.05), and no letter indicates no significant difference.

[0091] 1.7.2 Effects of Adding Balanced Oil Powder to Sow Diets on Sow Backfat

[0092] As Figure 1A to Figure 1C shown, adding balanced oil powder to the sow diet had no significant effect on sow backfat loss.

[0093] 1.7.3 Effects of Adding Balanced Oil Powder to Sows' Diets on Sows' Serum Biochemical Indicators

[0094] As can be seen from Table 4, adding balanced oil powder to the diet reduced the contents of cholesterol, triglyceride, high-density lipoprotein and uric acid in sows' plasma (P<0.05), and had no significant effect on indicators such as glutamic-oxaloacetic transaminase, total protein and albumin.

[0095] Table 4 Effects of Adding Balanced Oil Powder to Sows' Diets on Sows' Plasma Biochemical Indicators

[0096]

[0097] 1.7.4 Effects of Adding Balanced Oil Powder to Sows' Diets on Sows' Milk Composition and Immune Indicators

[0098] As Figure 2A to Figure 2C can be seen, adding balanced oil powder to the diet can significantly increase the contents of milk protein and non-fat solids in milk (P<0.05), significantly increase the contents of IgA and IgM in milk (P<0.05), and has no significant effect on milk fat.

[0099] Summary:

[0100] Feeding pregnant / lactating sows with a diet containing 2% balanced oil powder can improve the reproductive performance of sows and the growth performance of piglets.

[0101] Part II Protective Effect of Adding Balanced Oil Powder to Sows' Diets on Piglets' Intestinal Injury

[0102] This part studied the effect of adding balanced oil powder to sows' diets on the intestinal health of piglets. On the basis of Experiment 1, 12 healthy piglets (21 days old, body weight 5.80 ± 0.10 kg, a total of 24) were selected from the piglets born to two groups of sows according to the principle of similar body weight. An intestinal injury model was established by intraperitoneal injection of LPS to study the effect of adding balanced oil powder to sows' diets on the intestinal health of piglets. The 24 piglets were randomly divided into 4 groups according to the sow source according to a 2×2 factorial design, with 6 piglets in each group: ① Soybean oil group (piglets in the 2% soybean oil group, injected with normal saline); ② Balanced oil powder group (piglets in the 2% balanced oil powder group, injected with normal saline); ③ Soybean oil + LPS group (piglets in the 2% soybean oil group, injected with LPS); ④ Balanced oil powder + LPS group (piglets in the 2% balanced oil powder group, injected with LPS). The piglets were intraperitoneally injected with 100 μg / kg body weight of LPS or an equal amount of normal saline, and were slaughtered 4 hours later, and intestinal samples were taken for testing. The results showed that: (1) Feeding sows with diets containing 2% balanced oil powder significantly alleviated the decrease in jejunal villus height of piglets caused by LPS stimulation (P<0.05), and had no significant effect on crypt depth and the ratio of villus height to crypt depth. (2) Feeding sows with diets containing 2% balanced oil powder alleviated the decrease in the RNA / DNA ratio, the decrease in sucrase and maltase activities, and the decrease in the expression level of tight junction protein Claudin-1 in the jejunum of piglets caused by LPS (P<0.05). (3) Feeding sows with diets containing 2% balanced oil powder significantly reduced the increase in the expression levels of TNF-α, IL-1β and IL-6 in the jejunal mucosa of piglets caused by LPS stimulation (P<0.05). (4) Feeding sows with diets containing 2% balanced oil powder could significantly increase the mRNA expression levels of Mfn1 and Mfn2 in the jejunum of piglets (P<0.05), and increase the protein expression levels of Mfn1, Mfn2 and Opa1 in the jejunum of piglets (P<0.05). (5) Feeding sows with diets containing 2% balanced oil powder significantly inhibited the increase in the mRNA expression of apoptosis factors Caspase-3 and Caspase-9 in the jejunum of piglets after LPS stimulation (P<0.05). The above results indicate that feeding pregnant / lactating sows with diets containing 2% balanced oil powder can alleviate the intestinal structure and function damage of piglets caused by LPS, promote mitochondrial fusion of intestinal epithelial cells, and inhibit apoptosis of intestinal epithelial cells.

[0103] 2 Materials and methods

[0104] 2.1 Experimental materials

[0105] The experimental piglets had the same source as the sows in Experiment 1.

[0106] LPS (Escherichia coli serotype 055:B5, purity >99%; purchased from Sigma Chemical Company).

[0107] 2.2 Experimental design

[0108] At the end of Experiment 1, 12 healthy piglets (21 days old, body weight 5.80 ± 0.10 kg, 24 in total) were selected from the piglets born to the sows in both groups according to the principle of similar body weight. According to a 2×2 factorial design, they were randomly divided into 4 groups according to the source of the sows, with 6 replicates for each treatment and 1 pig per replicate. They were: ① Soybean oil group (Con), piglets in the 2% soybean oil group + intraperitoneal injection of normal saline; ② Balanced oil powder group (Bop), piglets in the 2% balanced oil powder group + intraperitoneal injection of normal saline; ③ Soybean oil + LPS group (Con+LPS), piglets in the 2% soybean oil group + intraperitoneal injection of LPS; ④ Balanced oil powder + LPS group (Bop+LPS), piglets in the 2% balanced oil powder group + intraperitoneal injection of LPS.

[0109] Piglets in the LPS group and the LPS + balanced oil powder group were intraperitoneally injected with 100 μg / kg body weight of LPS (Escherichia coli serotype 055:B5, purity > 99%; purchased from Sigma Chemical Company), and piglets in the control group and the balanced oil powder group were injected with an equal amount of 0.9% normal saline. All piglets were slaughtered 4 hours after LPS or saline treatment, and intestinal samples were taken for testing.

[0110] 2.3 Sample collection

[0111] 4 hours after injecting LPS or normal saline, the piglets were slaughtered. The jejunum was taken and rinsed with pre-cooled phosphate buffer saline (PBS). A 1-cm-long intestinal segment was taken from the middle of the jejunum and then fixed in 4% paraformaldehyde. Approximately 10 cm of the middle part of the jejunum was longitudinally opened and washed with PBS. Mucosal samples were collected from the jejunal tissue with a glass slide and quickly frozen in liquid nitrogen, and stored in a -80°C refrigerator for further analysis.

[0112] 2.4 Detection indexes and methods

[0113] 2.4.1 Intestinal morphology

[0114] The jejunal samples were fixed with 4% paraformaldehyde solution, and then made into intestinal sections for HE staining. The jejunal sections were observed morphologically using an HPIAS-1000 high-definition color pathological image report analysis system, and the villus height, crypt depth, and the ratio of villus height to crypt depth were calculated.

[0115] 2.4.2 Intestinal function

[0116] The contents of intestinal mucosal protein, DNA, and RNA were detected using a kit and a spectrophotometer. The specific method was referred to the experimental method of Wang Xiuying (2015).

[0117] The activities of jejunal lactase, sucrase and maltase were detected using kits A082-1-1, A082-2-1 and A082-3-1 produced by Nanjing Jiancheng Bioengineering Institute. The specific method was referred to the kit instruction manual.

[0118] The expression levels of intestinal tight junction proteins (Claudin-1, Occludin) were determined by Western blot method, with β-actin as the internal reference. The protein expression levels were represented by the ratios of the band intensities of Claudin-1 and Occludin to the band intensity of β-actin, respectively.

[0119] 2.4.3 Intestinal inflammatory response

[0120] mRNA in jejunal mucosa samples was extracted, and the expression levels of inflammatory cytokines (TNF-α, IL-6 and IL-1β) were analyzed by real-time fluorescence quantitative PCR. The internal reference gene was glyceraldehyde-3-phosphate dehydrogenase (GAPDH). The primer sequences are shown in Table 5.

[0121] Table 5 Primer sequence table

[0122]

[0123]

[0124] 2.4.4 mRNA and protein expressions of intestinal mitochondrial fusion-related regulatory proteins

[0125] The protein expressions of Mfn1, Mfn2 and Opa1 were determined in the same way as in Experiment 2.4.2, and the mRNA expression levels were detected in the same way as in Experiment 2.4.3.

[0126] 2.4.5 Intestinal cell apoptosis-related indicators

[0127] The detection method of the mRNA expression levels of Caspase-3, Caspase-8 and Caspase-9 was the same as that in Experiment 2.4.3.

[0128] 2.5 Statistical analysis

[0129] All data of the experiment were analyzed by one-way ANOVA using SPSS 22.0 statistical software and Duncan's multiple comparison. The statistical results were expressed as mean and standard error. A significant difference was indicated by P < 0.05.

[0130] 2.6 Experimental results

[0131] 2.6.1 Effects of adding balanced oil powder to sows' diets on the intestinal morphology of piglets

[0132] By Figure 3A andFigure 3B It can be seen that LPS stimulation led to a significant decrease in the jejunal villus height of piglets (P<0.05), and adding balanced oil powder to the sow diet could significantly alleviate the decrease in the jejunal villus height of piglets caused by LPS stimulation (P<0.05).

[0133] 2.6.2 Effects of adding balanced oil powder to the sow diet on intestinal repair, digestion and barrier functions of piglets

[0134] From Figure 4 it can be seen that adding balanced oil powder to the sow diet could significantly increase the ratio of RNA / DNA in the jejunal mucosa of piglets (P<0.05).

[0135] From Figure 5 it can be seen that adding balanced oil powder to the sow diet could significantly alleviate the decrease in the contents of maltase and sucrase in the jejunum of piglets caused by LPS stimulation (P<0.05); there was no significant effect on lactase.

[0136] From Figure 6A and Figure 6B it can be seen that adding balanced oil powder to the sow diet could significantly alleviate the decrease in the expression of tight junction protein Claudin-1 in the jejunum of piglets caused by LPS stimulation (P<0.05).

[0137] 2.6.3 Effects of adding balanced oil powder to the sow diet on intestinal inflammatory factors of piglets

[0138] From Figure 7 it can be seen that adding balanced oil powder to the sow diet could significantly alleviate the increase in the mRNA levels of intestinal inflammatory factors IL-1β, IL-6 and TNF-α in piglets caused by LPS stimulation (P<0.05).

[0139] 2.6.4 Effects of adding balanced oil powder to the sow diet on the mRNA and protein expression levels of mitochondrial fusion-related regulatory proteins in the intestine of piglets

[0140] From Figure 8A to Figure 8C it can be seen that adding balanced oil powder to the sow diet could significantly alleviate the decrease in the mRNA expression levels of Mfn1 and Mfn2 in the jejunal mucosa of piglets caused by LPS stimulation (P<0.05), and inhibit the decrease in the protein expression levels of Mfn1, Mfn2 and Opa1 in the jejunal mucosa of piglets caused by LPS stimulation (P<0.05).

[0141] 2.6.5 Effects of adding balanced oil powder to the sow diet on intestinal cell apoptosis-related indicators of piglets

[0142] From Figure 9It can be seen that adding balanced oil powder to the diet of sows can significantly alleviate the increase in the mRNA expression of Caspase-3 and Caspase-9 in the jejunal mucosa of piglets caused by LPS stimulation.

[0143] Discussion

[0144] The intestinal development of lactating piglets is not perfect, and they are easily stimulated by the outside world, resulting in intestinal damage due to stress, which affects their growth performance. The nutrition of lactating sows is directly related to the intestinal health of piglets. Experiment 1 found that adding oil powder to the diet of pregnant sows significantly increased milk production, improved milk composition and the growth performance of piglets.

[0145] In further experiments of the present invention, it was verified that the balanced oil powder has a positive effect on the intestinal development and intestinal health of piglets after acting on sows. In this application, an intestinal injury model was established by stimulating piglets with LPS to study the effect of adding oil powder to the diet of sows on the intestinal development and health of piglets. After the study, it was found that adding balanced oil powder to the diet of pregnant / lactating sows can alleviate the damage to the intestinal structure and function of piglets caused by LPS, promote the mitochondrial fusion of intestinal epithelial cells, and inhibit the apoptosis of intestinal epithelial cells.

[0146] Summary: In the present invention, balanced oil is applied to pregnant sows, and through experimental verification, it is found that it can effectively improve the reproductive performance of sows and the growth performance of piglets.

Claims

1. A balancing oil, characterized in that: The composition includes the following weight percentages: Fish oil 15-20wt%, soybean oil 20-30%, linseed oil 5-10%, lysophospholipid 20-30%, coconut oil 5-10%, corn oil 15-20%, red palm oil 1-5%.

2. A balanced oil powder, characterized in that: The invention comprises a carrier and the balancing oil as claimed in claim 1.

3. The balanced oil powder according to claim 2, characterized in that: The carrier is puffed corn or puffed soybean meal.

4. The balanced oil powder according to claim 2, characterized in that: The weight ratio of the carrier to the balancing oil is 1:

1.

5. Use of the balanced oil according to claim 1 or the balanced oil powder according to any one of claims 2 to 4 in preparing a sow feed additive.

6. The use according to claim 5, characterized in that The sow feed additive is used for reducing the content of cholesterol, triglyceride, high-density lipoprotein and uric acid in the plasma of the sow after feeding the sow, and / or increasing the content of milk protein and non-fat solids in the milk of the sow.

7. The use according to claim 5, characterized in that The sow feed additive is used to increase the number of weaned piglets, the weaning and rearing rate of piglets and the amount of milk secretion, increase the weaning litter weight and the weight gain of the weaning litter after feeding the sow, and reduce the delivery process.

8. The use according to claim 5, characterized in that The sow feed additive is used to improve the intestinal health of piglets after feeding the sow.

9. The use according to claim 5, characterized in that The sow is a pregnant sow.

10. The use according to claim 5, characterized in that The balanced oil is applied to sows from the gestation period to the lactation period.

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