Feed additive for improving transportation stress reaction of ruminants as well as preparation method and application of feed additive

Abstract

The invention provides a feed additive for improving transportation stress reaction of ruminants as well as a preparation method and application of the feed additive, and relates to the technical field of functional feed additives. The feed additive comprises a probiotic composition and a traditional Chinese medicine fermented material, the probiotic composition is composed of bacillus subtilis, enterococcus faecium and saccharomyces cerevisiae, and the traditional Chinese medicine fermented material is composed of codonopsis pilosula, fructus forsythiae, radix bupleuri, soybean meal and bran. The feed additive can influence the physiological indexes, biochemical indexes and blood indexes of the ruminants and promote the indexes to recover to the level before transportation, so that the transportation stress response of the ruminants is greatly improved.

Description

Technical Field

[0001] The present invention relates to the technical field of functional feed additives, and particularly relates to a feed additive for improving the transportation stress response of ruminants, a preparation method thereof, and an application thereof. Background Art

[0002] Livestock and poultry transportation is an essential and important link in the process of livestock husbandry production. Stressors such as crowding, jolting, noise, temperature changes, fasting, and water deprivation during transportation can easily lead to endocrine disorders in animals, manifested externally as intense reactions, fear or depression, increased aggression, accelerated heart and respiratory rates, and changes in body temperature. Some ruminants even experience diarrhea or constipation, and even death. Currently, the meat ruminant industry in China is developing rapidly, and the off-site fattening and cross-regional transportation of ruminants are becoming more and more frequent. The age of transported animals is getting smaller and the transportation radius is getting larger. Therefore, developing a feed additive that can improve the transportation stress of ruminants has good application prospects.

[0003] Currently, for anti-stress feeds and feed additives, research is usually carried out on physiological indexes, production performance indexes, etc., and less research is done on blood indexes.

[0004] In view of this, it is necessary to design an improved feed additive for improving the transportation stress response of ruminants, a preparation method thereof, and an application thereof to solve the above problems. Summary of the Invention

[0005] The purpose of the present invention is to provide a feed additive for improving the transportation stress response of ruminants, a preparation method thereof, and an application thereof.

[0006] To achieve the above-mentioned invention purpose, on the one hand, the present invention provides a feed additive for improving the transportation stress response of ruminants, comprising a probiotic composition and a traditional Chinese medicine fermented material;

[0007] The probiotic composition comprises raw materials in the following weight parts: 3 - 5 parts of Bacillus subtilis, 2 - 3 parts of Enterococcus faecium, and 2 - 3 parts of Saccharomyces cerevisiae;

[0008] The traditional Chinese medicine fermented material comprises raw materials in the following weight parts: 10 - 15 parts of Codonopsis pilosula, 5 - 10 parts of Forsythia suspensa, 3 - 5 parts of Bupleurum chinense, 3 - 5 parts of soybean meal, and 1 - 3 parts of wheat bran.

[0009] Preferably, the feed additive comprises a probiotic composition and a traditional Chinese medicine fermented material; the probiotic composition comprises raw materials in the following weight parts: 3 parts of Bacillus subtilis, 2 parts of Enterococcus faecium, and 2 parts of Saccharomyces cerevisiae; the traditional Chinese medicine fermented material comprises raw materials in the following weight parts: 10 parts of Codonopsis pilosula, 5 parts of Forsythia suspensa, 3 parts of Bupleurum chinense, 3 parts of soybean meal, and 1 part of wheat bran.

[0010] Preferably, the feed additive comprises a probiotic composition and a traditional Chinese medicine fermented material; the probiotic composition comprises raw materials in the following parts by weight: 4 parts of Bacillus subtilis, 2 parts of Enterococcus faecium, and 3 parts of Saccharomyces cerevisiae; the traditional Chinese medicine fermented material comprises raw materials in the following parts by weight: 12 parts of Codonopsis pilosula, 8 parts of Forsythia suspensa, 4 parts of Bupleurum chinense, 4 parts of soybean meal, and 2 parts of wheat bran.

[0011] Preferably, the feed additive comprises a probiotic composition and a traditional Chinese medicine fermented material; the probiotic composition comprises raw materials in the following parts by weight: 5 parts of Bacillus subtilis, 3 parts of Enterococcus faecium, and 3 parts of Saccharomyces cerevisiae; the traditional Chinese medicine fermented material comprises raw materials in the following parts by weight: 15 parts of Codonopsis pilosula, 10 parts of Forsythia suspensa, 5 parts of Bupleurum chinense, 5 parts of soybean meal, and 3 parts of wheat bran.

[0012] On the other hand, the present invention also provides a preparation method of the feed additive, comprising the following steps:

[0013] After the Bacillus, Enterococcus faecium, and Saccharomyces cerevisiae are subjected to seed preparation, they are respectively inoculated into a culture medium according to an inoculation amount of 0.1%. After the cultivation is completed, 3-5 parts of Bacillus subtilis, 2-3 parts of Enterococcus faecium, and 2-3 parts of Saccharomyces cerevisiae are mixed evenly according to parts by weight, and the probiotic mixture is mixed evenly with sterile water according to a mass ratio of 1:99 to obtain a probiotic composition;

[0014] 10-15 parts of Codonopsis pilosula, 5-10 parts of Forsythia suspensa, 3-5 parts of Bupleurum chinense, 3-5 parts of soybean meal, and 1-3 parts of wheat bran are mixed evenly according to parts by weight, pulverized and sieved to obtain a traditional Chinese medicine fermented material; the traditional Chinese medicine fermented material and the probiotic composition are mixed evenly according to a mass ratio of 1:(1.5-2), and fermented at 25-40 °C for 48 h; after the fermentation is completed, the mixture is dried to obtain the feed additive.

[0015] Preferably, the cultivation temperature of the Bacillus subtilis is 37 °C, and the cultivation time is 24 h; the cultivation temperature of the Enterococcus faecium and the Saccharomyces cerevisiae is 37 °C, and the cultivation time is 48 h.

[0016] Preferably, the viable count of the seed liquid obtained after the seed preparation of the Bacillus, the Enterococcus faecium, and the Saccharomyces cerevisiae is 1×10 7 -9×10 7 CFU / mL.

[0017] Preferably, the temperature during the drying process is 240-260 °C, and the time is 10-15 s.

[0018] Particularly, the feed additive prepared by the preparation method proposed by the present invention can be used to improve the transportation stress response of ruminants. The specific application method is as follows: The feed additive is mixed with the basal diet and continuously fed to the ruminants after transportation for 7 days; the concentrate-roughage ratio of the basal diet is 60:40.

[0019] The beneficial effects of the present invention are as follows:

[0020] The feed additive for improving the transportation stress response of ruminants provided by the present invention comprises a probiotic composition and a traditional Chinese medicine fermented material. The probiotic composition is composed of Bacillus subtilis, Enterococcus faecium and Saccharomyces cerevisiae, and the traditional Chinese medicine fermented material is composed of Codonopsis pilosula, Forsythia suspensa, Bupleurum chinense, soybean meal and wheat bran. When the feed additive with the above composition is applied to ruminants after transportation, it can affect the physiological indexes (body temperature, respiration, pulse, feed intake), biochemical indexes (aldosterone, cortisol), and blood indexes (number of white blood cells, red blood cells and hemoglobin, hematocrit) of ruminants, and promote the above indexes to return to the level before transportation, greatly improving the transportation stress response of ruminants. Detailed implementation manners

[0021] In order to make the objectives, technical solutions and advantages of the present invention clearer, the present invention will be described in detail below with reference to specific embodiments.

[0022] Here, it should also be noted that in order to avoid obscuring the present invention due to unnecessary details, only the structures and / or processing steps closely related to the solution of the present invention are shown in the text, while other details less related to the present invention are omitted.

[0023] In addition, it should also be noted that the term "comprises", "comprising" or any other variant thereof is intended to cover a non-exclusive inclusion, such that a process, method, article or device comprising a series of elements not only includes those elements but also includes other elements not expressly listed, or further includes elements inherent to such process, method, article or device.

[0024] On the one hand, the present invention provides a feed additive for improving the transportation stress response of ruminants, comprising a probiotic composition and a traditional Chinese medicine fermented material; the probiotic composition comprises raw materials in the following weight parts: 3-5 parts of Bacillus subtilis, 2-3 parts of Enterococcus faecium, 2-3 parts of Saccharomyces cerevisiae, and the traditional Chinese medicine fermented material comprises raw materials in the following weight parts: 10-15 parts of Codonopsis pilosula, 5-10 parts of Forsythia suspensa, 3-5 parts of Bupleurum chinense, 3-5 parts of soybean meal, 1-3 parts of wheat bran.

[0025] On the other hand, the present invention also provides a preparation method of the above feed additive for improving the transportation stress response of ruminants, comprising the following steps:

[0026] The freeze-dried powders of Bacillus, Enterococcus faecium, and Saccharomyces cerevisiae were inoculated into the Bacillus subtilis medium, lactic acid bacteria medium, and fungal medium respectively at an inoculation amount of 0.1%. The culture temperature of Bacillus subtilis was 37°C and the culture time was 24 h. The culture temperatures of Enterococcus faecium and Saccharomyces cerevisiae were 37°C and the culture time was 48 h. After the culture, 3 - 5 parts by weight of Bacillus subtilis, 2 - 3 parts by weight of Enterococcus faecium, and 2 - 3 parts by weight of Saccharomyces cerevisiae were mixed evenly, and the probiotic mixture was mixed evenly with sterile water at a mass ratio of 1:99 to obtain a probiotic composition. Among them, 10 - 15 parts by weight of Codonopsis pilosula, 5 - 10 parts by weight of Forsythia suspensa, 3 - 5 parts by weight of Bupleurum chinense, 3 - 5 parts by weight of soybean meal, and 1 - 3 parts by weight of wheat bran were mixed evenly, pulverized and sieved to obtain a traditional Chinese medicine fermentation material. After mixing the traditional Chinese medicine fermentation material and the probiotic composition evenly at a mass ratio of 1:(1.5 - 2), it was fermented at 25 - 40°C for 48 h. After the fermentation, the mixture was dried to obtain a feed additive.

[0027] As an embodiment of the present invention, the viable count of the seed liquid obtained after the seed production of Bacillus, Enterococcus faecium, and Saccharomyces cerevisiae is 1×10 7 -9×10 7 CFU / mL.

[0028] As an embodiment of the present invention, before mixing the traditional Chinese medicine fermentation material and the probiotic composition, the traditional Chinese medicine fermentation material needs to be sterilized, cooled to room temperature after the treatment, and then mixed with the probiotic composition. The sterilization treatment can be carried out as follows: high-temperature steam sterilization at 121°C for 30 min. In some other embodiments, other sterilization methods can also be used as long as the sterilization purpose can be achieved, which will not be elaborated here.

[0029] As an embodiment of the present invention, the temperature during the drying process is 240 - 260°C and the time is 10 - 15 s.

[0030] Furthermore, the present invention also provides an application method of the above-mentioned feed additive for improving the transportation stress response of ruminants, including the following steps: mixing the basal diet and the feed additive of the present invention evenly at a ratio of 99.5:0.5, continuously feeding the ruminants after transportation for 7 days, allowing free access to food and water during this period, and adding feed in a timely manner according to the feed intake. The basal diet is prepared according to the feeding standard of ruminants, and the concentrate-to-forage ratio is 60:40. After mixing the feed additive proposed by the present invention with the basal diet and feeding the ruminants before transportation, it can effectively avoid stress reactions of ruminants such as cattle and sheep during transportation.

[0031] The following further illustrates the feed additive for improving the transportation stress response of ruminants proposed by the present invention, its preparation method and application with specific examples:

[0032] Example 1

[0033] In this example, a feed additive for improving the transportation stress response of ruminants was prepared, which includes a probiotic composition and a traditional Chinese medicine fermentation material; the probiotic composition comprises raw materials in the following weight parts: 3 parts of Bacillus subtilis, 2 parts of Enterococcus faecium, and 2 parts of Saccharomyces cerevisiae, and the traditional Chinese medicine fermentation material includes raw materials in the following weight parts: 10 parts of Codonopsis pilosula, 5 parts of Forsythia suspensa, 3 parts of Bupleurum chinense, 3 parts of soybean meal, and 1 part of wheat bran.

[0034] The specific preparation method is as follows:

[0035] Bacillus subtilis, Enterococcus faecium, and Saccharomyces cerevisiae were respectively inoculated into a Bacillus subtilis medium, a lactic acid bacteria medium, and a fungal medium at an inoculation amount of 0.1%. Bacillus subtilis was cultured at 37°C for 24 h, and Enterococcus faecium and Saccharomyces cerevisiae were cultured at 37°C for 48 h. Then, 3 parts of Bacillus subtilis, 2 parts of Enterococcus faecium, and 2 parts of Saccharomyces cerevisiae were mixed evenly according to the weight parts to obtain the probiotic composition; among them, the viable count of the seed liquid obtained after seed production was 1×10 7 CFU / mL;

[0036] 10 parts of Codonopsis pilosula, 5 parts of Forsythia suspensa, 3 parts of Bupleurum chinense, 3 parts of soybean meal, and 1 part of wheat bran were mixed evenly according to the weight parts, pulverized and sieved through a 40-mesh sieve, and then sterilized with high-temperature steam at 121°C for 30 min. After the sterilization was completed, the temperature was lowered to 37°C, and it was mixed with the probiotic composition and fermented at 35°C for 48 h. After the fermentation was completed, it was dried at 240°C for 15 s to obtain the feed additive.

[0037] Example 2

[0038] In this example, a feed additive for improving the transportation stress response of ruminants was prepared, which includes a probiotic composition and a traditional Chinese medicine fermentation material; the probiotic composition comprises raw materials in the following weight parts: 4 parts of Bacillus subtilis, 2 parts of Enterococcus faecium, and 3 parts of Saccharomyces cerevisiae, and the traditional Chinese medicine fermentation material includes raw materials in the following weight parts: 12 parts of Codonopsis pilosula, 8 parts of Forsythia suspensa, 4 parts of Bupleurum chinense, 4 parts of soybean meal, and 2 parts of wheat bran.

[0039] The specific preparation method is as follows:

[0040] Bacillus subtilis, Enterococcus faecium, and Saccharomyces cerevisiae were respectively inoculated into a Bacillus subtilis medium, a lactic acid bacteria medium, and a fungal medium at an inoculation amount of 0.1%. Bacillus subtilis was cultured at 37°C for 24 h, and Enterococcus faecium and Saccharomyces cerevisiae were cultured at 37°C for 48 h. Then, 4 parts of Bacillus subtilis, 2 parts of Enterococcus faecium, and 3 parts of Saccharomyces cerevisiae were mixed evenly according to the weight parts to obtain the probiotic composition; among them, the viable count of the seed liquid obtained after seed production was 5×10 7 CFU / mL;

[0041] Mix 12 parts of Codonopsis pilosula, 8 parts of Forsythia suspensa, 4 parts of Bupleurum chinense, 4 parts of soybean meal, and 2 parts of wheat bran evenly by weight, crush them, sieve them through a 40-mesh sieve, then sterilize them with high-temperature steam at 121 °C for 30 min. After sterilization, cool them down to 37 °C, mix them with the probiotic composition, and ferment them at 35 °C for 48 h. After fermentation, dry them at 250 °C for 12 s to obtain the feed additive.

[0042] Example 3

[0043] In this example, a feed additive for improving the transportation stress response of ruminants was prepared, which included a probiotic composition and a traditional Chinese medicine fermented material; the probiotic composition included the following raw materials by weight: 5 parts of Bacillus subtilis, 3 parts of Enterococcus faecium, and 3 parts of Saccharomyces cerevisiae. The traditional Chinese medicine fermented material included the following raw materials by weight: 15 parts of Codonopsis pilosula, 10 parts of Forsythia suspensa, 5 parts of Bupleurum chinense, 5 parts of soybean meal, and 3 parts of wheat bran.

[0044] The specific preparation method is as follows:

[0045] Bacillus subtilis, Enterococcus faecium, and Saccharomyces cerevisiae were inoculated into the Bacillus subtilis medium, lactic acid bacteria medium, and fungal medium respectively at an inoculation amount of 0.1%. Bacillus subtilis was cultured at 37 °C for 24 h, and Enterococcus faecium and Saccharomyces cerevisiae were cultured at 37 °C for 48 h. Then, 5 parts of Bacillus subtilis, 3 parts of Enterococcus faecium, and 3 parts of Saccharomyces cerevisiae were mixed evenly by weight to obtain the probiotic composition; among them, the viable count of the seed liquid obtained after seed production was 9×10 7 CFU / mL;

[0046] Mix 10 parts of Codonopsis pilosula, 5 parts of Forsythia suspensa, 3 parts of Bupleurum chinense, 3 parts of soybean meal, and 1 part of wheat bran evenly by weight, crush them, sieve them through a 40-mesh sieve, then sterilize them with high-temperature steam at 121 °C for 30 min. After sterilization, cool them down to 37 °C, mix them with the probiotic composition, and ferment them at 35 °C for 48 h. After fermentation, dry them at 260 °C for 10 s to obtain the feed additive.

[0047] Application Examples 1 to 3

[0048] In this part of the examples, Hu sheep was used as the experimental object to explore the effect of the feed additive prepared in Application Examples 1 - 3 on the stress resistance of Hu sheep during transportation. The experimental design was as follows:

[0049] Select 80 Hu sheep at 4 months old, with a body weight of 27 ± 2 kg and good body condition as the test sheep. Load the test sheep onto the vehicle and transport them to the breeding farm. The maximum speed during transportation is 70 km / h, and the transportation time is 8 h. Do not feed but allow water intake 6 h before transportation, do not feed or drink water during transportation, and do not feed but allow water intake after arrival. After arriving at the breeding farm, randomly divide all the Hu sheep into 4 groups, with 20 sheep in each group, namely the control group, Experimental Group 1, Experimental Group 2, and Experimental Group 3;

[0050] On the day when the experimental sheep arrived at the feeding farm, they were led into the isolation sheep house, disinfected, and fed a small amount of feed and light salt water. On the second day, the experimental groups 1-3 began to be fed the mixed feed obtained by mixing the basal diet and the feed additive of the present invention. The mass ratio of the basal diet to the feed additive was 95.5:0.5. The control group was only fed the basal diet and fed continuously for 7 days. Except for the different feeds fed, the other feeding conditions of the control group and the experimental groups 1-3 were the same. They were fed at 6:00 and 18:00 every day, with free access to food and water, and the feed was added in a timely manner according to the feed intake. During the experiment, the respiration, body temperature, pulse, and body weight of the fasting experimental sheep were measured before transportation, on the 1st day, 3rd day, and 7th day of starting feeding. At the same time, venous blood was collected. 4 mL of blood was collected from each sheep and divided into 2 tubes, 2 mL each. After one blood sample coagulated, it was centrifuged at a low temperature of 3500 rpm for 15 min, and the upper serum was aspirated and stored at 4°C for measuring the content of aldosterone and cortisol. The other blood sample was anticoagulated with sodium heparin and stored at 4°C, and the blood routine was measured within 24 h. Measuring instrument: Hematology Analyzer (SYSMEX XE2100).

[0051] Among them, the basal diet was formulated with reference to the feeding standard for meat sheep (NY / T 816-2021), and the concentrate-to-forage ratio was 60:40. Its composition and nutritional level are shown in Table 1. Among them, the premix in the table provided per kilogram of diet: vitamin A 10000 IU, vitamin D3 1000 IU, vitamin E 100 mg, niacin 30 mg, Fe 32 mg, Cu 5 mg, Zn 18.5 mg, Mn 10 mg, I 0.5 mg, Co 0.1 mg; the digestible energy in the nutritional level was a calculated value, and the rest were measured values. The apparent digestible energy (MJ / kg) of the diet = (total energy ingested - total energy in feces) / weight of feed ingested.

[0052] Table 1 Composition and nutritional level of the basal diet

[0053]

[0054] After the transportation was completed, the experimental sheep showed symptoms such as increased body temperature, accelerated respiration and pulse, and decreased feed intake. After 7 days of feeding, the above symptoms in the control group fed with the basal diet still could not recover to the level before transportation. After 3 days of feeding in the experimental groups 1-3, the body temperature and pulse decreased to varying degrees, but were still higher than the normal values. They completely returned to normal on the 7th day. The respiratory rate and feed intake could return to the level before transportation on the 3rd day. The results of the physiological indexes of the experimental sheep before transportation and during the feeding process are shown in Table 2. The symbol * in the table indicates a significant difference compared with the control group.

[0055] Table 2 Results of the physiological indexes of the experimental sheep before transportation and during the feeding process

[0056]

[0057]

[0058] The results of the biochemical indexes of the experimental sheep before transportation and during feeding are shown in Table 3. The levels of cortisol and aldosterone reflect the strength of the stress level in the sheep. When the sheep is in a stress state, the contents of aldosterone and cortisol in the body increase and exceed the normal range. On the 7th day, the contents of aldosterone and cortisol in the control group were still at a relatively high level, higher than the average value. The aldosterone and cortisol in the experimental group decreased significantly during the experimental period and returned to the level before transportation on the 7th day, indicating that the stress state of the sheep was relieved. Among them, the symbol * in the table indicates a significant difference compared with the control group (p < 0.5), and the symbol ** indicates a very significant difference compared with the control group (p < 0.05).

[0059] Table 3 Results of the biochemical indexes of the experimental sheep before transportation and during feeding

[0060]

[0061] The changes in the blood indexes of the experimental sheep before transportation and during feeding are shown in Table 4. The white blood cells increased after transportation. The increase in white blood cells is a significant sign of the stress response. In the stress response, the oxygen consumption of a large number of activated white blood cells increases, and most of the consumed oxygen is generated into oxygen free radicals (O 2- ) by single-electron reduction and combines with polyunsaturated fatty acids on the cell membrane to generate lipid peroxides, which damage the cell membrane structure and function, affect the phospholipase activity, promote the decomposition of arachidonic acid in phospholipids to produce thromboxane A2, resulting in increased vascular permeability, causing tissue edema, and then tissue damage. Compared with the control group, the white blood cell count in experimental groups 1-3 was reduced to the level before transportation within 7 days, which is beneficial to alleviating the negative effects brought by the increase in white blood cells caused by transportation stress. In the transportation stress response, the juxtaglomerular apparatus is stimulated by stressors to release erythropoietin, which converts hepatic erythrogenin into erythropoietin, accelerates the maturation of red blood cells and releases them into the circulatory system. The increase in red blood cells, hemoglobin, and hematocrit can increase the oxygen-carrying capacity to adapt to the large oxygen consumption conditions of increased respiration, elevated blood pressure, muscle tension, and enhanced catabolism during the stress response, and meet the needs of tissue cell activities. However, if the red blood cells, hemoglobin, and hematocrit continue to increase excessively, it will also cause vascular occlusion, hypoxia, and tissue damage. Compared with the control group, the red blood cell count, hemoglobin, and hematocrit indexes in experimental groups 1-3 were reduced to the level before transportation within 7 days, which is beneficial to alleviating the negative effects brought by the increase in white blood cells caused by transportation stress.

[0062] Table 4 Changes in the blood indexes of the experimental sheep before transportation and during feeding

[0063]

[0064] The above embodiments are only used to illustrate the technical solutions of the present invention and not to limit them. Although the present invention has been described in detail with reference to the preferred embodiments, those of ordinary skill in the art should understand that the technical solutions of the present invention can be modified or equivalently replaced without departing from the spirit and scope of the technical solutions of the present invention.

Claims

1. A feed additive for improving transportation stress response of ruminants, characterized in that: Including probiotic composition and traditional Chinese medicine fermentation material; The probiotic composition comprises the following raw materials in parts by weight: 3-5 parts of Bacillus subtilis, 2-3 parts of Enterococcus faecium, and 2-3 parts of Saccharomyces cerevisiae; The traditional Chinese medicine fermentation material comprises the following raw materials in parts by weight: 10-15 parts of Codonopsis pilosula, 5-10 parts of Forsythia suspensa, 3-5 parts of Bupleurum chinense, 3-5 parts of soybean meal, and 1-3 parts of bran.

2. The feed additive according to claim 1, characterized in that The feed additives include a probiotic composition and a traditional Chinese medicine fermentation feed; The probiotic composition comprises the following raw materials in parts by weight: 3 parts of Bacillus subtilis, 2 parts of Enterococcus faecium, and 2 parts of Saccharomyces cerevisiae; The traditional Chinese medicine fermentation material comprises the following raw materials in parts by weight: 10 parts of Codonopsis pilosula, 5 parts of Forsythia suspensa, 3 parts of Bupleurum chinense, 3 parts of soybean meal and 1 part of bran.

3. The feed additive according to claim 1, characterized in that The feed additives include a probiotic composition and a traditional Chinese medicine fermentation feed; The probiotic composition comprises the following raw materials in parts by weight: 4 parts of Bacillus subtilis, 2 parts of Enterococcus faecium, and 3 parts of Saccharomyces cerevisiae; The traditional Chinese medicine fermentation material comprises the following raw materials in parts by weight: 12 parts of Codonopsis pilosula, 8 parts of Forsythia suspensa, 4 parts of Bupleurum chinense, 4 parts of soybean meal and 2 parts of bran.

4. The feed additive according to claim 1, characterized in that The feed additives include a probiotic composition and a traditional Chinese medicine fermentation feed; The probiotic composition comprises the following raw materials in parts by weight: 5 parts of Bacillus subtilis, 3 parts of Enterococcus faecium, and 3 parts of Saccharomyces cerevisiae; The traditional Chinese medicine fermentation material comprises the following raw materials in parts by weight: 15 parts of Codonopsis pilosula, 10 parts of Forsythia suspensa, 5 parts of Bupleurum chinense, 5 parts of soybean meal and 3 parts of bran.

5. A method for preparing the feed additive according to claim 1, characterized in that: The steps include: After the production of seeds, Bacillus, Enterococcus faecium and Saccharomyces cerevisiae are inoculated into the culture medium at an inoculum rate of 0.1% respectively. After the culture is completed, 3-5 parts of Bacillus subtilis, 2-3 parts of Enterococcus faecium and 2-3 parts of Saccharomyces cerevisiae are mixed evenly according to weight parts, and the probiotic mixture is mixed evenly with sterile water at a mass ratio of 1:99 to prepare a probiotic composition; Mix 10-15 parts of Codonopsis pilosula, 5-10 parts of Forsythia suspensa, 3-5 parts of Bupleurum chinense, 3-5 parts of soybean meal and 1-3 parts of bran evenly according to weight, grind and sieve to obtain Chinese medicine fermentation material; mix the Chinese medicine fermentation material and the probiotic composition evenly according to the mass ratio of 1: (1.5-2), and ferment at 25-40°C for 48h; after the fermentation is completed, dry the mixture to obtain the feed additive.

6. The preparation method according to claim 5, characterized in that: The culture temperature of the Bacillus subtilis is 37° C., and the culture time is 24 hours; the culture temperature of the Enterococcus faecium and the Saccharomyces cerevisiae is 37° C., and the culture time is 48 hours.

7. The preparation method according to claim 5, characterized in that: The number of live bacteria in the seed liquid obtained after the Bacillus, Enterococcus faecium and Saccharomyces cerevisiae seed production is 1×10 7 -9×10 7 CFU / mL.

8. The preparation method according to claim 5, characterized in that: The temperature of the drying process is 240-260°C and the time is 10-15s.

9. Use of the feed additive prepared by the preparation method according to any one of claims 5 to 8 in improving transportation stress response of ruminants.

10. A method for using the feed additive prepared by the preparation method according to any one of claims 5 to 8, characterized in that: The method is as follows: the feed additive is mixed with the basic feed, and the mixture is continuously fed to the ruminants after transportation for 7 days; the concentrate-roughage ratio of the basic feed is 60:40.

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