Rat and mouse chronic constraint stress combined sound, light and electricity and electromagnetic wave stimulation device

Abstract

The invention relates to a rat and mouse chronic constraint stress combined acousto-optic-electric and electromagnetic wave stimulation device, and belongs to the field of experimental equipment. Comprising a hollow binding box, a collecting box used for collecting excrement is inserted into the bottom of the binding box, a positioning frame is arranged at the top end of the collecting box, and a plurality of electric wires are fixedly arranged on the inner wall of the positioning frame and used for electrically shocking mice; a cover plate is hinged to the top of the constraint box, a plurality of partition plates are adjustably arranged in the constraint box, a plurality of constraint spaces are formed between every two adjacent partition plates and between the partition plates and the constraint box, and acousto-optic stimulation modules and electromagnetic wave emission modules corresponding to the constraint spaces are fixedly arranged at the bottom of the cover plate. Through flexible partition plate adjustment, automatic binding belt fixation and a multi-mode stimulation system, efficient and accurate binding and stimulation of experimental mice are achieved, meanwhile, the stability and easy-to-clean performance of the experimental environment are ensured, and the experimental efficiency and data accuracy are greatly improved.

Description

Technical Field

[0001] The present invention belongs to the field of experimental equipment and relates to a device for chronic restraint stress combined with acoustic, optical, and electromagnetic wave stimulation for rats and mice. Background Art

[0002] Currently, chronic stress models are widely used in animal behavior and neuroscience research, especially in pharmacology and stress research. Existing stress model devices mostly use a single stimulation mode (such as restraint stress or acoustic and optical stimulation), and there are certain limitations in the flexibility of the stimulation method, stimulation intensity, and duration.

[0003] After retrieval, the invention with the publication number CN114651782B discloses a method and device for establishing a chronic restraint stress model for mice. The device also has the following disadvantages during use: When the mouse is placed in the restraint box during use, it can only limit the activity range of the mouse and cannot fix the mouse, affecting the safety of the experiment; during the experiment, the feces of the mouse will remain on the partition, and the partition is not easy to disassemble, making subsequent cleaning difficult. Summary of the Invention

[0004] In view of this, in order to solve the problems that when the mouse is placed in the restraint box during use, it can only limit the activity range of the mouse and cannot fix the mouse, affecting the safety of the experiment; during the experiment, the feces of the mouse will remain on the partition, and the partition is not easy to disassemble, making subsequent cleaning difficult, the present invention provides a device for chronic restraint stress combined with acoustic, optical, and electromagnetic wave stimulation for rats and mice.

[0005] To achieve the above object, the present invention provides the following technical solutions:

[0006] A device for chronic restraint stress combined with acoustic, optical, and electromagnetic wave stimulation for rats and mice, including a restraint box. The restraint box is hollow, and a collection box for collecting feces is inserted at the bottom of the restraint box. A positioning frame is provided at the top of the collection box, and a plurality of electric wires are fixedly arranged on the inner wall of the positioning frame for electrocuting the mouse.

[0007] A cover plate is hinged to the top of the restraint box. A plurality of partitions are adjustably arranged in the restraint box, and a plurality of restraint spaces are formed between adjacent two partitions and between the partition and the restraint box. An acoustic and optical stimulation module and an electromagnetic wave emission module corresponding to the restraint space are fixedly arranged at the bottom of the cover plate for acoustic and optical stimulation of the mouse. A lock is provided on one side of the restraint box for fixing the cover plate. A door opening corresponding to the restraint space is provided on one side of the restraint box, a door frame is provided outside the door opening, and a door body is inserted into the door frame.

[0008] A fixing mechanism is arranged on one side of the restraint box, and it is used in cooperation with the cover plate to fix the adjusted restraint space.

[0009] A restraint mechanism is provided in the restraint space, which is used to fix mice of different sizes without affecting the adjustment of the restraint space;

[0010] An electromagnetic wave stimulation host is provided on one side of the restraint box, on which an acoustic-optical stimulation host is provided. The electromagnetic wave stimulation host is connected to the electromagnetic wave transmitting module via a wire, and the acoustic-optical stimulation host is connected to the acoustic-optical stimulation module via a wire for controlling the working state.

[0011] Furthermore, the restraining mechanism includes a first installation frame corresponding to the restraining space, and multiple first installation frames are respectively fixed on one side of the partition and the restraining box. A first rotating shaft is rotatably provided in the first installation frame, and a second winding roller is fixedly sleeved on the outer wall of the first rotating shaft. A restraining belt is fixed and wrapped around the outer wall of the second winding roller, and the other end of the restraining belt is fixedly connected to the corresponding partition and the restraining box. Waist-shaped holes are opened on both sides of the restraining box, and the first rotating shaft is located in the two waist-shaped holes. A driving assembly is provided on one side of the restraining box for driving the first rotating shaft to reel in the restraining belt.

[0012] Furthermore, a second mounting frame is fixedly provided on one side of the partition and the restraining box, two third rotating shafts are rotatably penetrated in the second mounting frame, a first winding roller is fixedly sleeved on the outer wall of the third rotating shaft, a pull rope is fixedly provided on the outer wall of the first winding roller and wound around, two connecting blocks are provided on one side of the second mounting frame, the other end of the pull rope penetrates one side of the second mounting frame and is fixedly connected to the corresponding connecting blocks, the middle section of the restraining belt is located in the two connecting blocks, and a torsion spring is sleeved on the outer wall of the third rotating shaft, and the two ends of the torsion spring are respectively fixedly connected to the inner wall of one side of the second mounting frame and one side of the first winding roller.

[0013] Furthermore, the driving assembly includes a transmission shaft rotatably arranged on one side of the restraint box, a motor is fixedly arranged on one side of the restraint box, an output end of the motor is fixedly connected to the transmission shaft, one end of the first rotating shaft is transmission-connected to the transmission shaft through a connecting member, and does not affect the rotation of a single first rotating shaft.

[0014] Furthermore, the connecting part includes an electromagnetic clutch, and the input end of the electromagnetic clutch and the outer wall of the transmission shaft are both sleeved with two meshing second gears, the outer wall of the electromagnetic clutch is fixedly sleeved with a second mounting seat, the bottom end of the second mounting seat is rotatably sleeved on the outer wall of the second gear on the transmission shaft, the second gear located on the transmission shaft is slidingly sleeved on the transmission shaft, the second gear located on the electromagnetic clutch is fixedly sleeved on the input end of the electromagnetic clutch, the output end of the electromagnetic clutch and the outer wall of the first rotating shaft are both fixedly sleeved with meshing first gears, the top end of the electromagnetic clutch is fixedly sleeved with a first mounting seat, the top end of the first mounting seat is rotatably sleeved on the first rotating shaft, and a plurality of switches are fixedly sleeved on one side of the restraint box, and the switches are connected to the corresponding electromagnetic clutches through wires.

[0015] Furthermore, the fixing mechanism includes a fixing strip fixed on one side of the restraining box, two guide rods are slidably provided on the top of the fixing strip, the same sliding rod is fixedly provided on the top of the two guide rods, a spring is sleeved on the outer wall of the guide rod, two ends of the spring are respectively fixedly connected to the fixed strip and the side of the sliding rod close to each other, wedge strips that interfere with the cover plate are fixedly provided on both sides of the sliding rod, and a second pressure strip that interferes with the multiple first mounting seats is fixedly provided on one side of one of the wedge strips.

[0016] Furthermore, a sliding block is rotatably sleeved on the outer wall of the other end of the first rotating shaft, and the sliding block is slidably arranged on one side of the restraining box. A first pressure strip that contacts with multiple sliding blocks is fixedly arranged on one side of another wedge strip.

[0017] Furthermore, a plurality of mounting posts are fixedly provided on the top of the collection box, a mounting hole which is plugged into the mounting post is provided in the restraining box, an L-shaped post is slidably provided in the mounting hole, an outer wall fixing sleeve of the L-shaped post is provided with a limiting ring located in the mounting hole, an outer wall sleeve of the L-shaped post is provided with a tension spring located in the mounting hole, two ends of the tension spring are respectively fixedly connected to one side of the limiting ring and one side inner wall of the mounting hole, a socket corresponding to the L-shaped post is provided on the outer wall of the mounting post, and a plurality of L-shaped posts are all transmission-connected to the wedge bar.

[0018] Furthermore, a sliding bar used in conjunction with a wedge bar is fixedly disposed on one end of a plurality of L-shaped plug posts on the same side.

[0019] Furthermore, guide rails are fixedly provided on the inner walls of both sides of the restraint box, and a plurality of partitions are slidably sleeved on the two guide rails. The guide rails are hollow and used for storing food, and a plurality of holes for feeding are opened on the top thereof.

[0020] The beneficial effects of the present invention are:

[0021] 1. The chronic restraint stress combined with sound, light, and electromagnetic wave stimulation device for mice and rats disclosed in the present invention can flexibly adjust the spacing between adjacent partitions according to the size of the experimental mice by driving the first rotating shaft and the partition through the sliding block, thereby ensuring that mice of different sizes can be effectively restrained and fixed, thereby improving the applicability and accuracy of the experiment;

[0022] 2. The chronic restraint stress combined with acoustic, optical and electromagnetic wave stimulation device for mice and rats disclosed in the present invention utilizes the cooperation of the L-shaped plug post and the plug hole, as well as the reset mechanism of the spring and the tension spring, to achieve a stable connection between the restraint box and the collection box, thereby ensuring the stability of the device during the experiment, avoiding experimental errors caused by shaking or displacement, and facilitating the cleaning of the collection box to avoid bacterial infection of subsequent experimental animals;

[0023] 3. A device for chronic restraint stress combined with acousto-optic and electromagnetic wave stimulation of mice and rats disclosed by the present invention realizes the automatic fixation and release of the restraint belt on the abdomen of the mouse through the coordinated use of a motor, a transmission shaft, an electromagnetic clutch, and a winding roller, which not only reduces the operation burden of the experimenter, but also improves the efficiency and accuracy of fixation, ensuring the consistency and repeatability of the experiment.

[0024] 3. A device for chronic restraint stress combined with acousto-optic and electromagnetic wave stimulation of mice and rats disclosed by the present invention locks the cover plate through a buckle to prevent accidental opening; at the same time, the designs of the wedge bar, the first pressing bar, and the second pressing bar also ensure the stability of the first rotating shaft and the electromagnetic clutch in the fixed state, reducing potential safety hazards during the operation process.

[0025] The present invention realizes the efficient and precise restraint and stimulation of experimental mice through flexible partition adjustment, automated restraint belt fixation, and a multi-modal stimulation system, while ensuring the stability and easy cleaning of the experimental environment, greatly improving the experimental efficiency and data accuracy.

[0026] Other advantages, objectives, and features of the present invention will be described to some extent in the subsequent specification, and to some extent, will be obvious to those skilled in the art based on the study of the following text, or can be learned from the practice of the present invention. The objectives and other advantages of the present invention can be realized and obtained through the following specification. BRIEF DESCRIPTION OF THE DRAWINGS

[0027] In order to make the objectives, technical solutions, and advantages of the present invention clearer, the present invention will be described in detail preferably with reference to the accompanying drawings, where:

[0028] Figure 1 is a three-dimensional structure schematic diagram of a device for chronic restraint stress combined with acousto-optic and electromagnetic wave stimulation of mice and rats according to the present invention;

[0029] Figure 2 is a three-dimensional structure schematic diagram of another perspective of a device for chronic restraint stress combined with acousto-optic and electromagnetic wave stimulation of mice and rats according to the present invention;

[0030] Figure 3 is a schematic diagram of the open structure of the restraint box of a device for chronic restraint stress combined with acousto-optic and electromagnetic wave stimulation of mice and rats according to the present invention;

[0031] Figure 4 is a schematic diagram of the installation structure of the partition and the restraint belt of a device for chronic restraint stress combined with acousto-optic and electromagnetic wave stimulation of mice and rats according to the present invention;

[0032] Figure 5 is a schematic diagram of the sectional structure of the installation frame of a device for chronic restraint stress combined with acousto-optic and electromagnetic wave stimulation of mice and rats according to the present invention;

[0033] Figure 6 Schematic diagram of the installation structure of the collection box and the positioning frame of a chronic restraint stress combined with acousto-optic and electromagnetic wave stimulation device for rats and mice according to the present invention;

[0034] Figure 7 Partial cross-sectional structure schematic diagram of the restraint box of a chronic restraint stress combined with acousto-optic and electromagnetic wave stimulation device for rats and mice according to the present invention;

[0035] Figure 8 is Figure 2 Schematic diagram of the enlarged structure of part A in

[0036] Reference numerals: 1, restraint box; 2, cover plate; 3, collection box; 4, door frame; 5, door body; 6, waist-shaped hole; 7, first rotating shaft; 8, sliding block; 9, first pressing strip; 10, wedge strip; 11, lock; 12, acousto-optic stimulation host; 13, electromagnetic wave stimulation host; 14, fixing strip; 15, sliding rod; 16, guiding rod; 17, spring; 18, second pressing strip; 19, sliding strip; 20, transmission shaft; 21, motor; 22, switch; 23, guide rail; 24, partition board; 25, restraint space; 26, restraint belt; 27, acousto-optic stimulation module; 28, electromagnetic wave emission module; 29, first mounting frame; 30, second mounting frame; 31, third rotating shaft; 32, connecting block; 33, first winding roller; 34, torsion spring; 35, mounting column; 36, positioning frame; 37, electric wire; 38, L-shaped plug post; 39, mounting hole; 40, limiting ring; 41, tension spring; 42, jack; 43, first mounting seat; 44, electromagnetic clutch; 45, first gear; 46, second mounting seat; 47, second gear; 48, second winding roller; 49, pulling rope. Detailed implementation manners

[0037] The following specifically illustrates the implementation manners of the present invention through specific examples. Those skilled in the art can easily understand other advantages and effects of the present invention from the content disclosed in this specification. The present invention can also be implemented or applied through other different specific implementation manners. Various details in this specification can also be modified or changed based on different viewpoints and applications without departing from the spirit of the present invention. It should be noted that the diagrams provided in the following embodiments only illustrate the basic concept of the present invention in a schematic manner. Without conflict, the following embodiments and the features in the embodiments can be combined with each other.

[0038] As Figures 1-8A device for chronic restraint stress combined with acoustic, optical, and electromagnetic wave stimulation for rats and mice, including a restraint box 1. The restraint box 1 is hollow, providing a closed test environment for rats and mice and being light-impermeable. The bottom of the restraint box 1 is designed with a slot for inserting a collection box 3. The collection box 3 is used to collect feces generated during the test, facilitating subsequent cleaning and research. The top of the collection box 3 is provided with a positioning frame 36, and a plurality of electric wires 37 are fixedly installed on the inner wall of the positioning frame 36. These electric wires 37 can be used to give electric shock stimulation to the rats when needed to simulate a specific stress environment.

[0039] A cover plate 2 is hinged to the top of the restraint box 1. The cover plate 2 can be opened or closed for internal maintenance. A plurality of partition plates 24 are adjustably arranged in the restraint box 1. The partition plates 24 can slide in the restraint box 1 to adjust the size of the restraint space 25 to meet the experimental needs of rats of different sizes. A plurality of restraint spaces 25 are formed between two adjacent partition plates 24 and between the partition plates 24 and the restraint box 1. The bottom of the cover plate 2 is fixedly provided with an acoustic and optical stimulation module 27 and an electromagnetic wave emission module 28 corresponding to the restraint space 25. These modules can give multiple stimulations of sound, light, and electromagnetic waves to the rats during operation. A lock 11 is provided on one side of the restraint box 1 for fixing the cover plate 2 to ensure safety during the experiment. A door opening corresponding to the restraint space 25 is also provided on one side of the restraint box 1. A door frame 4 is provided outside the door opening, and a door body 5 is inserted into the door frame 4. The door body 5 can be opened or closed, facilitating the operation of the experimenter.

[0040] An electromagnetic wave stimulation host 13 is provided on one side of the restraint box 1. An acoustic and optical stimulation host 12 is provided on the electromagnetic wave stimulation host 13. The electromagnetic wave stimulation host 13 is connected to the electromagnetic wave emission module 28 through a wire, and the acoustic and optical stimulation host 12 is connected to the acoustic and optical stimulation module 27 through a wire for controlling the working state. The acoustic and optical stimulation host 12 uses the ZK-CJY acoustic and optical stimulator of Beijing Zhecheng Technology Co., Ltd., and the electromagnetic wave stimulation host 13 and the electromagnetic wave emission module 28 respectively adopt the data processing device and signal emission component in the patent document CN 211086637 U.

[0041] In order to fix the adjusted restraint space 25, a fixing mechanism is provided on one side of the restraint box 1. The fixing mechanism includes a fixing strip 14 fixedly arranged on one side of the restraint box 1. Two guide rods 16 are slidably arranged through the top of the fixing strip 14. The same sliding rod 15 is fixedly arranged at the top of the guide rods 16. A spring 17 is sleeved on the outer wall of the guide rods 16. The two ends of the spring 17 are fixedly connected to the mutually close sides of the fixing strip 14 and the sliding rod 15 respectively, providing a restoring force for the sliding rod 15. Wedge strips 10 that are in contact with the cover plate 2 are fixedly arranged on both sides of the sliding rod 15. A second pressing strip 18 is fixedly arranged on one side of one of the wedge strips 10. The second pressing strip 18 is used to be in contact with a plurality of first mounting seats 43 to fix the position of the partition plate 24.

[0042] In the restraint space 25, a restraint mechanism is provided for fixing mice of different sizes. The restraint mechanism includes a first mounting frame 29 corresponding to the restraint space 25. A first rotating shaft 7 is rotatably arranged through the first mounting frame 29. A second winding roller 48 is fixedly sleeved on the outer wall of the first rotating shaft 7. A restraint belt 26 is fixedly wound around the outer wall of the second winding roller 48. The other end of the restraint belt 26 is fixedly connected to the corresponding partition plate 24 and the restraint box 1 to form a C shape for restraining the mouse. Waist-shaped holes 6 are opened on both sides of the restraint box 1. The first rotating shaft 7 is located in the two waist-shaped holes 6, allowing the first rotating shaft 7 to move within the length range of the waist-shaped holes 6. A driving assembly is arranged on one side of the restraint box 1 for driving the first rotating shaft 7 to wind the restraint belt 26, thereby restraining the mouse.

[0043] The driving assembly includes a transmission shaft 20 rotatably arranged on one side of the restraint box 1. A motor 21 is fixedly arranged on one side of the restraint box 1. The output end of the motor 21 is fixedly connected to the transmission shaft 20. One end of the first rotating shaft 7 is in transmission connection with the transmission shaft 20 through a connecting member without affecting the rotation of a single first rotating shaft 7. The connecting member is specifically an electromagnetic clutch 44. Meshing second gears 47 are sleeved on the input end of the electromagnetic clutch 44 and the outer wall of the transmission shaft 20 respectively. A second mounting seat 46 is fixedly sleeved on the outer wall of the electromagnetic clutch 44. The bottom end of the second mounting seat 46 is rotatably sleeved on the outer wall of the second gear 47 on the transmission shaft 20. The second gear 47 on the transmission shaft 20 is slidably sleeved on the transmission shaft 20 (horizontally slidably connected through a slider guide rail). The second gear 47 on the electromagnetic clutch 44 is fixedly sleeved on the input end of the electromagnetic clutch 44. Meshing first gears 45 are fixedly sleeved on the output end of the electromagnetic clutch 44 and the outer wall of the first rotating shaft 7 respectively. A first mounting seat 43 is fixedly arranged at the top of the electromagnetic clutch 44. The top end of the first mounting seat 43 is rotatably sleeved on the first rotating shaft 7. A plurality of switches 22 are fixedly arranged on one side of the restraint box 1. The switches 22 are connected to the corresponding electromagnetic clutches 44 through wires for controlling the working state of the electromagnetic clutches 44, thereby controlling the winding of the corresponding restraint belts 26.

[0044] In order to further enhance the binding effect, a second mounting frame 30 is fixedly provided on one side of the partition 24 and the binding box 1. Two third rotating shafts 31 are rotatably provided in the second mounting frame 30. A first winding roller 33 is fixedly provided on the outer wall of the third rotating shaft 31. A pull rope 49 is fixedly provided and wound around the outer wall of the first winding roller 33. Two connecting blocks 32 are provided on one side of the second mounting frame 30. The other end of the pull rope 49 passes through one side of the second mounting frame 30 and is fixedly connected to the corresponding connecting block 32. The middle section of the binding belt 26 is located in the two connecting blocks 32. A torsion spring 34 is provided on the outer wall of the third rotating shaft 31. The two ends of the torsion spring 34 are respectively fixedly connected to the inner wall of one side of the second mounting frame 30 and one side of the first winding roller 33, providing a reset force for the pull rope 49, so that the pull rope 49 can be automatically tightened when not acted upon by external force, so that the binding belt 26 can be C-shaped and will not change, and the binding belt 26 can be further fixed.

[0045] The outer wall of the other end of the first rotating shaft 7 is rotatably sleeved with a sliding block 8, which is slidably arranged on one side of the restraining box 1. A first pressure strip 9 is fixedly arranged on one side of another wedge strip 10, and the first pressure strip 9 is used to interfere with multiple sliding blocks 8 to further fix the position of the first rotating shaft 7.

[0046] A plurality of mounting posts 35 are fixedly provided on the top of the collection box 3, and a mounting hole 39 for plugging with the mounting posts 35 is provided in the restraining box 1. An L-shaped plug post 38 is slidably provided in the mounting hole 39, and a limit ring 40 located in the mounting hole 39 is fixedly provided on the outer wall of the L-shaped plug post 38, and a tension spring 41 located in the mounting hole 39 is provided on the outer wall of the L-shaped plug post 38. The two ends of the tension spring 41 are respectively fixedly connected to one side of the limit ring 40 and one side inner wall of the mounting hole 39, so as to provide reset for the L-shaped plug post 38. A plug hole 42 corresponding to the L-shaped plug post 38 is provided on the outer wall of the force mounting post 35. In order to realize the centralized control of the L-shaped plug post 38, one end of the multiple L-shaped plug posts 38 on the same side is fixedly provided with the same sliding bar 19, and the sliding bar 19 is transmission-connected with the wedge bar 10. When the wedge bar 10 moves, it can drive the sliding bar 19 to move, and then drive the multiple L-shaped plug posts 38 to move synchronously, so as to realize the rapid disassembly and assembly of the collection box 3.

[0047] Guide rails 23 are fixedly provided on the inner walls of both sides of the restraint box 1, and a plurality of partitions 24 are slidably sleeved on the two guide rails 23, so as to facilitate the sliding adjustment of the partitions 24 in the restraint box 1. The guide rails 23 are hollow and used to store food, and a plurality of holes for feeding are provided on the top thereof, so as to provide food for the mice during the experiment and avoid the influence of hunger on the experimental results.

[0048] During use, power on the device, place the restraint box 1 on the collection box 3, and insert the mounting post 35 into the corresponding mounting hole 39. Then, pull the door body 5 upward to open the entrance on one side of the restraint box 1. Next, place the experimental mouse through the entrance into the corresponding restraint space 25 inside the restraint box 1. After placing the mouse, close the door body 5. Then, push the sliding block 8 to move. The movement of the sliding block 8 can drive the first rotating shaft 7 to move. The movement of the first rotating shaft 7 can drive the corresponding partition 24 to move through the first mounting frame 29, and adjust the distance between two adjacent partitions 24 according to the size of the mouse. At this time, the mouse is located inside the restraint belt 26. At the same time, install the external data acquisition system in the corresponding restraint space 25;

[0049] During the movement of the first rotating shaft 7, it can drive the electromagnetic clutch 44 to move through the first mounting seat 43. The movement of the electromagnetic clutch 44 can drive the second gear 47 to move on the transmission shaft 20 through the second mounting seat 46;

[0050] After the adjustment is completed, close the cover plate 2. During the closing process of the cover plate 2, it can drive the two wedge bars 10 to move downward. The downward movement of the wedge bars 10 can drive the sliding rod 15 to move downward and compress the spring 17. And the downward movement of the wedge bars 10 can drive the sliding strip 19 to move. The movement of the sliding strip 19 can drive the L-shaped plug post 38 to move, so that the L-shaped plug post 38 is inserted into the corresponding jack 42, and the restraint box 1 can be fixed on the collection box 3. During the movement of the L-shaped plug post 38, it can stretch the tension spring 41 through the limit ring 40. After the cover plate 2 is completely closed, it is locked by the lock catch 11;

[0051] At the same time, during the downward movement of the wedge bars 10, it can drive the first pressure bar 9 to abut against the multiple first rotating shafts 7, and the second pressure bar 18 to abut against the multiple first mounting seats 43 to fix the position of the first rotating shaft 7;

[0052] Then, start the audio-visual-electrical stimulation host 12 to control the audio-visual stimulation module 27 and the electric wire 37 to perform audio-visual-electrical stimulation on the mouse respectively, and start the electromagnetic wave stimulation host 13 to control the electromagnetic wave emission module 28 to perform electromagnetic wave stimulation on the mouse. And use the data acquisition system to record the behavioral responses of the mouse during the stimulation process in real time. And there is also a rolling curtain outside the door body 5, which can block the light from entering the restraint box 1 to facilitate dark stimulation;

[0053] And physical objects can be placed in the guide rail 23. When the mouse is hungry, it can obtain the physical objects through the holes on the guide rail 23;

[0054] When it is necessary to fix the mouse, start the motor 21 to drive the transmission shaft 20 to rotate. The rotation of the transmission shaft 20 can drive the input end of the electromagnetic clutch 44 to rotate through the meshing second gear 47, and then start the corresponding electromagnetic clutch 44 through the switch 22. When the electromagnetic clutch 44 is started, the input end of the electromagnetic clutch 44 can drive the output end to rotate, so as to drive the first rotating shaft 7 to rotate through the meshing first gear 45. The rotation of the first rotating shaft 7 can drive the second winding roller 48 to rotate, and take in one end of the restraint belt 26. The middle section of the restraint belt 26 will approach the mouse, and cooperate with the partition plate 24 and the restraint box 1 to clamp and fix the mouse. During the movement of the middle section of the restraint belt 26, it can also pull the pull rope 49 on the first winding roller 33 to move outward through the connecting block 32. During the movement of the pull rope 49, it can drive the third rotating shaft 31 to rotate through the first winding roller 33 and compress the torsion spring 34 until the mouse's abdomen is completely fixed;

[0055] After the experiment is completed, open the cover plate 2. At this time, the sliding rod 15 can move upward and reset under the action of the spring 17, and drive the wedge 10 to move upward and reset, releasing the fixation of the partition plate 24. At the same time, the L-shaped plug 38 can move reset under the action of the tension spring 41, so that one end of the L-shaped plug 38 disengages from the jack 42, and drive the sliding strip 19 to move reset. Then, pull the restraint box 1 upward, and the restraint box 1 can be separated from the collection box 3, and then the collection box 3 can be cleaned.

[0056] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and are not intended to limit them. Although the present invention has been described in detail with reference to the preferred embodiments, those of ordinary skill in the art should understand that the technical solutions of the present invention can be modified or equivalently replaced without departing from the purpose and scope of the present technical solution, and they should all be covered within the scope of the claims of the present invention.

Claims

1. A chronic restraint stress combined with acoustic, optical and electromagnetic wave stimulation device for mice and rats, characterized in that: The invention comprises a restraint box (1), wherein the restraint box (1) is hollow, a collecting box (3) for collecting feces is plugged into the bottom of the restraint box (1), a positioning frame (36) is provided at the top of the collecting box (3), and a plurality of electric wires (37) are fixedly provided on the inner wall of the positioning frame (36) for electric shocking mice; A cover plate (2), the cover plate (2) being hinged on the top of a restraint box (1), the restraint box (1) being provided with a plurality of partition plates (24) which can be adjusted, a plurality of restraint spaces (25) being formed between two adjacent partition plates (24) and between the partition plates (24) and the restraint box (1), a sound and light stimulation module (27) and an electromagnetic wave emission module (28) corresponding to the restraint spaces (25) being fixedly provided at the bottom of the cover plate (2) for providing sound and light stimulation to mice, a lock buckle (11) for fixing the cover plate (2) being provided on one side of the restraint box (1), a door opening corresponding to the restraint space (25) being provided on one side of the restraint box (1), a door frame (4) being provided on the outer side of the door opening, and a door body (5) being inserted into the door frame (4); A fixing mechanism is arranged on one side of the restraining box (1) and is used in conjunction with the cover plate (2) to fix the adjusted restraining space (25); A restraining mechanism is disposed in the restraining space (25) and is used to fix mice of different sizes without affecting the adjustment of the restraining space (25); An electromagnetic wave stimulation host (13) is provided on one side of the restraint box (1), an acoustic and optical stimulation host (12) is provided on the electromagnetic wave stimulation host (13), the electromagnetic wave stimulation host (13) is connected to an electromagnetic wave emission module (28) via a wire, and the acoustic and optical stimulation host (12) is connected to an acoustic and optical stimulation module (27) via a wire, for controlling the working state.

2. The chronic restraint stress combined with acoustic, optical and electromagnetic wave stimulation device for mice and rats as claimed in claim 1, characterized in that: The restraining mechanism comprises a first mounting frame (29) corresponding to the restraining space (25), and a plurality of the first mounting frames (29) are respectively fixedly arranged on one side of the partition (24) and the restraining box (1); a first rotating shaft (7) is rotatably provided inside the first mounting frame (29); a second winding roller (48) is fixedly sleeved on the outer wall of the first rotating shaft (7); a restraining belt (26) is fixedly arranged and wound around the outer wall of the second winding roller (48); the other end of the restraining belt (26) is fixedly connected to the corresponding partition (24) and the restraining box (1); waist-shaped holes (6) are provided on both sides of the restraining box (1); the first rotating shaft (7) is located in the two waist-shaped holes (6); and a driving component is provided on one side of the restraining box (1) for driving the first rotating shaft (7) to reel in the restraining belt (26).

3. The chronic restraint stress combined with acoustic, optical and electromagnetic wave stimulation device for mice and rats as claimed in claim 2, characterized in that: A second mounting frame (30) is fixedly provided on one side of the partition (24) and the restraining box (1), two third rotating shafts (31) are rotatably provided in the second mounting frame (30), a first winding roller (33) is fixedly sleeved on the outer wall of the third rotating shaft (31), a pull rope (49) is fixedly provided on the outer wall of the first winding roller (33), two connecting blocks (32) are provided on one side of the second mounting frame (30), the other end of the pull rope (49) passes through one side of the second mounting frame (30) and is fixedly connected to the corresponding connecting block (32), the middle section of the restraining belt (26) is located in the two connecting blocks (32), and a torsion spring (34) is sleeved on the outer wall of the third rotating shaft (31), and the two ends of the torsion spring (34) are respectively fixedly connected to the inner wall of one side of the second mounting frame (30) and one side of the first winding roller (33).

4. The chronic restraint stress combined with acoustic, optical and electromagnetic wave stimulation device for mice and rats as claimed in claim 3, characterized in that: The driving assembly comprises a transmission shaft (20) rotatably arranged on one side of the restraining box (1); a motor (21) is fixedly arranged on one side of the restraining box (1); an output end of the motor (21) is fixedly connected to the transmission shaft (20); one end of the first rotating shaft (7) is transmission-connected to the transmission shaft (20) via a connecting member, and does not affect the rotation of a single first rotating shaft (7).

5. The chronic restraint stress combined with acoustic, optical and electromagnetic wave stimulation device for mice and rats as claimed in claim 4, characterized in that: The connecting member comprises an electromagnetic clutch (44); the input end of the electromagnetic clutch (44) and the outer wall of the transmission shaft (20) are both sleeved with two meshing second gears (47); the outer wall of the electromagnetic clutch (44) is fixedly sleeved with a second mounting seat (46); the bottom end of the second mounting seat (46) is rotatably sleeved on the outer wall of the second gear (47) on the transmission shaft (20); the second gear (47) on the transmission shaft (20) is slidably sleeved on the transmission shaft (20); the second gear (47) on the electromagnetic clutch (44) is sleeved with the outer wall of the second gear (47) on the transmission shaft (20); A gear (47) is fixedly sleeved on the input end of the electromagnetic clutch (44); the output end of the electromagnetic clutch (44) and the outer wall of the first rotating shaft (7) are both fixedly sleeved with a first gear (45) that meshes with each other; a first mounting seat (43) is fixedly provided on the top end of the electromagnetic clutch (44); the top end of the first mounting seat (43) is rotatably sleeved on the first rotating shaft (7); a plurality of switches (22) are fixedly provided on one side of the restraint box (1); and the switches (22) are connected to the corresponding electromagnetic clutches (44) through wires.

6. The chronic restraint stress combined with acoustic, optical and electromagnetic wave stimulation device for mice and rats as claimed in claim 2, characterized in that: The fixing mechanism comprises a fixing bar (14) fixed on one side of the restraining box (1), two guide rods (16) are slidably provided through the top of the fixing bar (14), the top of the two guide rods (16) are fixedly provided with a same sliding rod (15), the outer wall of the guide rod (16) is sleeved with a spring (17), the two ends of the spring (17) are respectively fixedly connected to the fixing bar (14) and the sliding rod (15) on the side close to each other, the two sides of the sliding rod (15) are fixedly provided with wedge strips (10) that abut against the cover plate (2), and one side of one of the wedge strips (10) is fixedly provided with a second pressure strip (18) that abuts against the plurality of first mounting seats (43).

7. The chronic restraint stress combined with acoustic, optical and electromagnetic wave stimulation device for mice and rats as claimed in claim 6, characterized in that: A sliding block (8) is rotatably sleeved on the outer wall of the other end of the first rotating shaft (7), and the sliding block (8) is slidably arranged on one side of the restraining box (1). A first pressure strip (9) that contacts the plurality of sliding blocks (8) is fixedly arranged on one side of the other wedge strip (10).

8. The chronic restraint stress combined with acoustic, optical and electromagnetic wave stimulation device for mice and rats as claimed in claim 7, characterized in that: A plurality of mounting posts (35) are fixedly provided on the top of the collection box (3); a mounting hole (39) for plugging into the mounting post (35) is provided in the restraining box (1); an L-shaped plug post (38) is slidably provided in the mounting hole (39); a limit ring (40) located in the mounting hole (39) is fixedly sleeved on the outer wall of the L-shaped plug post (38); a tension spring (41) located in the mounting hole (39) is sleeved on the outer wall of the L-shaped plug post (38); two ends of the tension spring (41) are respectively fixedly connected to one side of the limit ring (40) and the inner wall of one side of the mounting hole (39); a plug hole (42) corresponding to the L-shaped plug post (38) is provided on the outer wall of the mounting post (35); and a plurality of the L-shaped plug posts (38) are all transmission-connected to the wedge bar (10).

9. The chronic restraint stress combined with acoustic, optical and electromagnetic wave stimulation device for mice and rats as claimed in claim 8, characterized in that: A common sliding bar (19) used in conjunction with the wedge bar (10) is fixedly provided at one end of the plurality of L-shaped plug posts (38) on the same side.

10. The chronic restraint stress combined with acoustic, optical and electromagnetic wave stimulation device for mice and rats as claimed in claim 9, characterized in that: Guide rails (23) are fixedly provided on the inner walls of both sides of the restraint box (1), and a plurality of partitions (24) are slidably sleeved on the two guide rails (23). The guide rails (23) are hollow and used for storing food, and a plurality of holes for feeding are provided on the top thereof.

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