A multifunctional mouse stress model establishment device

Abstract

The present application relates to the technical field of biological experiment apparatus, disclose a kind of multifunctional mouse stress model establishment device, comprising: box frame and feeding mechanism, restraint mechanism, illumination mechanism, temperature control mechanism, acupuncture mechanism and control center;Box frame is by mutually perpendicular connection crossbeam, stringer and vertical beam, horizontal placement base plate is connected between vertical beam, the periphery of base plate is provided with inner layer breathable baffle, and inner layer breathable baffle constitutes mouse feeding space with base plate;Mouse is restrained by restraint mechanism and carries out restraint stress experiment, mouse is carried out by illumination mechanism and carries out high light stress experiment, mouse is carried out by temperature control mechanism and carries out cold and hot stress experiment, mouse is carried out by acupuncture mechanism and carries out foot bottom acupuncture and electric shock stress experiment.The device of the present application integrates the function of a variety of stress stimulation experiments, while realizing the components or mechanisms of each stress function can be coordinated with each other, can also be independent, facilitate simultaneously experiment one or multiple stress stimulation, wide application range.

Description

A multifunctional mouse stress model establishment device Technical Field

[0001] This invention relates to the field of biological experimental equipment technology, and in particular to a multifunctional mouse stress model establishment device. Background Technology

[0002] Because mice (and rats), as mammals, share many diseases with humans and have a high degree of DNA affinity with humans, they are frequently used in various drug and stress experiments. There are many types of stress experiments on mice (and rats), common ones including restraint stress experiments (for establishing a depression model), strong light stress experiments, needle prick stress experiments, foot electric shock stress experiments, and hot / cold stress experiments. These stress experiments can reveal abnormal phenomena and behaviors exhibited by mice under specific stimuli, which has certain reference value for the study of human characteristics.

[0003] Based on existing technologies, most of the devices used to establish mouse (rat) stress models are single-function and cannot perform multiple stress experiments simultaneously. Furthermore, conducting different types of experiments requires transferring the mice (rat) to different experimental devices, which is not only cumbersome but also affects the observation of the mice's stress response. Summary of the Invention

[0004] This invention provides a multifunctional mouse stress model establishment device, which solves the problem of limited functionality in existing stress model devices.

[0005] A multifunctional mouse stress model establishment device includes: a box frame and a feeding mechanism, a restraint mechanism, a lighting mechanism, a temperature control mechanism, a needle pricking mechanism and a control center disposed on the box frame;

[0006] The box frame is composed of horizontal beams, longitudinal beams and vertical beams that are perpendicularly connected to each other. A horizontally placed base plate is connected between the vertical beams. An inner breathable partition is set around the base plate. The inner breathable partition and the base plate together form the mouse breeding space. An outer sealing partition is set outside the inner breathable partition. A pinhole plate is slidably set under the base plate.

[0007] Mice were subjected to restraint stress experiments using a restraint mechanism, strong light stress experiments using a light exposure mechanism, environmental temperature change stress experiments using a temperature control mechanism, and foot acupuncture and electric shock stress experiments using a needle puncture mechanism.

[0008] The beneficial effects of adopting the above technical solution are as follows: The device of the present invention is equipped with a restraint mechanism, a light-illuminating mechanism, a temperature control mechanism and a needle-puncturing mechanism, which can complete one or more mouse stress experiments separately or simultaneously, overcoming the problem of the single function of existing stress model devices, greatly improving experimental efficiency, and reducing experimental costs and procedures; the various mechanisms of the device of the present invention are relatively independent, and other components or mechanisms will not cause interference when a single stress experiment is carried out, and the device is simple to use and operate with high observation accuracy.

[0009] Furthermore, the aforementioned feeding mechanism includes a water trough and a feed trough, with the water trough connected to a water supply pipe and the feed trough connected to a feeding channel;

[0010] The water pipe is equipped with a heating outer layer on the outside and the bottom of the water tank. The heating outer layer is equipped with a heating element inside. The water tank is equipped with a water temperature thermometer for measuring water temperature and a water level gauge for measuring water depth.

[0011] The heating element, water temperature gauge, water level gauge, and switches on the water supply pipe are all connected to the control center.

[0012] The beneficial effects of adopting the above technical solution are as follows: food can be conveniently fed into the feed trough through the feeding channel without opening the box; water can be added directly from the outside of the box to the inside through the water supply pipe, and heating elements are installed on both the water supply pipe and the water trough, so that clean water can be delivered smoothly even when the ambient temperature is below zero, so that the mice can drink. In addition, a water temperature meter and a water level meter are installed in the water trough, which can monitor the drinking process and time of the mice in real time, so as to facilitate accurate analysis by the experimental operators.

[0013] Furthermore, the aforementioned restraint mechanism includes a binding strap, a slide rail, and a slider, with the slider slidably mounted on the slide rail;

[0014] The slide rail includes a transverse slide rail and a longitudinal slide rail. The transverse slide rail is set on the inner breathable partition, and the slider on the transverse slide rail is connected to the left sliding plate and the right sliding plate. The longitudinal slide rail is set on the left sliding plate or the right sliding plate, and the slider on the longitudinal slide rail is connected to the front sliding plate and the rear sliding plate.

[0015] The binding straps are connected to the left sliding plate, the right sliding plate, the front sliding plate, and the rear sliding plate. The ends of the binding straps are connected to buckles, and the lower half of the sliding plate is provided with buckle blocks, with the buckles fastened to the buckle blocks.

[0016] The beneficial effects of adopting the above technical solution are as follows: The restraint mechanism is used for restraint stress experiments on mice. The restraint mechanism includes a restraint strap, a slide rail slider, and a sliding plate. The slide rail slider and sliding plate can drive the mice from a large mouse breeding space to a smaller area, which is convenient for experimental operators to capture and restrain. At the same time, when restraining the mice, it can also restrict their movement in a small area, overcoming the shortcomings of existing restraint devices that are not secure.

[0017] Furthermore, the aforementioned lighting mechanism includes a lamp tube disposed below the cover and a light sensor disposed outside the inner breathable partition, the light sensor being communicatively connected to the control center.

[0018] The beneficial effects of adopting the above technical solution are: setting up a light-emitting mechanism facilitates strong light stress experiments on mice, and the light sensor can measure the different light intensity coefficients that cause different degrees of stimulation to mice, which is conducive to quantifying experimental results.

[0019] Furthermore, the aforementioned temperature control mechanism includes an air conditioner and a temperature sensor. The air conditioner's outlet is located within the mouse rearing space, and the temperature sensor is located outside the inner breathable partition. The air conditioner and the temperature sensor are respectively connected to the control center.

[0020] The beneficial effects of adopting the above technical solution are as follows: the temperature of the mouse breeding space can be locally regulated by the temperature control mechanism, which can realize the stress response of mice under cold, hot or alternating cold and hot conditions, avoiding the problem that existing experimental devices can only be carried out in a specific large-space laboratory.

[0021] Furthermore, the aforementioned needle-punching mechanism includes a sliding plate and needles disposed on the upper surface of the sliding plate, the distribution of which is adapted to the through-holes on the needle plate; the sliding plate is raised and lowered by a lifting rod.

[0022] The beneficial effects of adopting the above technical solution are as follows: Acupuncture stress experiments can be conducted on mice using a movable needle, and foot shock experiments can be conducted on mice by connecting an electric wire to the needle and making it conductive.

[0023] Furthermore, the bottom of each of the aforementioned vertical beams is located on a weight transmitter, which is connected to a weighing sensor, and the weighing sensor is communicatively connected to the control center.

[0024] The beneficial effects of adopting the above technical solution are as follows: the vertical beam of the box is set on the weight transmitter, which can monitor the weight changes of the box and the mice in real time, and provide weight change data for various stress experiments.

[0025] Furthermore, the side of the substrate is provided with a fecal collection trough, which is arranged at an angle and the outlet end is located outside the outer sealing partition.

[0026] Furthermore, multiple cameras are installed on the aforementioned enclosure frame, with the camera lenses facing the mouse rearing space, and the cameras are connected to the control center.

[0027] The beneficial effects of adopting the above technical solution are: the camera can record mice around the clock, providing a basis for studying the dynamic behavior of mice, while also avoiding long-term on-site monitoring by experimental personnel, which helps to save the physical strength and time of experimental operators.

[0028] Furthermore, both the inner breathable partition and the outer sealing partition are equipped with boxes and doors, which are movably connected to boxes and boxes changing channels.

[0029] The beneficial effects of adopting the above technical solution are: the feeding box of mice can be changed conveniently through the box changing channel, which can be done without manual capture.

[0030] The present invention has the following beneficial effects:

[0031] (1) The stress model establishment device of the present invention integrates the functions of various stress stimulation experiments. At the same time, the components or mechanisms that realize each stress function can coordinate with each other or be independent of each other, which facilitates the simultaneous experimentation of one or more stress stimuli. It has complete functions and a wide range of applications.

[0032] (2) The stress model establishment device of the present invention can set up one or more devices to conduct experiments according to specific experimental conditions and environment. The experimental site can be remotely observed and remotely operated through the control center, which is conducive to conducting stress experiments on a large scale, in a cluster, and in different regions.

[0033] (3) The stress model establishment device of the present invention has a simple structure and low manufacturing cost. It is easy for experimental operators to use. At the same time, the use of a camera to replace manual monitoring avoids the problem of existing experimental devices requiring long-term on-site observation by personnel. Attached Figure Description

[0034] Figure 1 is a schematic diagram of the external structure of the device of the present invention;

[0035] Figure 2 is a schematic diagram of the internal structure of the device of the present invention after concealing the outer sealing partition, the binding mechanism, the needle-punching mechanism and the inner breathable partitions on the front right sides.

[0036] Figure 3 is a schematic diagram of the structure of the box frame in the device of the present invention;

[0037] Figure 4 is a partially enlarged schematic diagram of the feeding mechanism in the invention device;

[0038] Figure 5 is a radial cross-sectional view of the water supply pipe and the heating outer layer in the device of the present invention;

[0039] Figure 6 is a schematic diagram of the pinhole plate in the device of the present invention;

[0040] Figure 7 is a schematic diagram of the restraint mechanism in the device of the present invention;

[0041] Figure 8 is a schematic diagram of the acupuncture mechanism in the device of the present invention.

[0042] In the diagram: 10-Box frame; 101-Crossbeam; 102-Longitudinal beam; 103-Vertical beam; 104-Inner breathable partition; 105-Outer sealing partition; 106-Base plate; 107-Pinhole plate; 108-Manure collection trough; 109-Box door; 110-Box cover; 20-Feeding mechanism; 201-Water trough; 202-Water pipe; 203-Heating outer layer; 204-Water thermometer; 205-Water level gauge; 206-Feed trough; 207-Feeding channel; 30-Constraint mechanism; 301-Slide rail; 302-Slider; 303-Front sliding plate; 304-Rear sliding plate; 305-Left sliding plate; 306-Right sliding plate; 307-Binding strap; 308-Buckle block; 309-Ring buckle; 40-Illumination mechanism; 401-Lamp tube; 402-Light sensor; 501-Air conditioner; 502-Temperature sensor; 60-Needle-piercing mechanism; 601-Needle; 602-Slide plate; 603-Lifting rod; 70-Camera; 80-Control center; 90-Weighing sensor; 901-Weight transmitter. Detailed Implementation

[0043] The principles and features of the present invention are described below with reference to the accompanying drawings. The examples given are only for explaining the present invention and are not intended to limit the scope of the present invention.

[0044] Referring to Figures 1 to 6, the present invention provides a multifunctional mouse stress model establishment device, including: a box frame 10 and a feeding mechanism 20, a restraint mechanism 30, a lighting mechanism 40, a temperature control mechanism, a needle puncture mechanism 60, a camera 70 and a control center 80 disposed on the box frame 10.

[0045] The box frame 10 is composed of horizontal beams 101, longitudinal beams 102 and vertical beams 103 that are perpendicularly connected to each other. The beams are connected by welding or by screws / bolts through screw holes. The beams can be made of square steel pipes, angle steel or other materials. In this embodiment, square steel pipes are selected.

[0046] To facilitate the measurement of mouse weight changes, weight transmitters 901 are installed at the bottom of the four vertical beams 103. That is, the weight of the vertical beams 103, other components arranged on the vertical beams 103, and the weight of the fed mice all act on the weight transmitters 901. The weight transmitters 901 are connected to the weighing sensors 90, which are communicatively connected to the control center 80. The weighing sensors 90 transmit the mouse weight changes in real time to the control center 80 and save them for easy viewing and retrieval by the experimenters to analyze the mouse weight changes in various stress experiments.

[0047] A base plate 106 is provided in the middle of the vertical beam 103. The base plate 106 is a rectangular flat plate with four sides connected to the vertical beam 103 and placed horizontally, providing basic support for the mouse sample space. An inner breathable partition 104 is provided around the base plate 106. The inner breathable partition 104 is vertically arranged, with its two sides connected to the vertical beam 103 and its bottom edge connected to the base plate 106. The inner breathable partition 104 and the base plate 106 together form the mouse breeding space. The lower half of the inner breathable partition 104 is a sealing plate, and the upper half of the plate has multiple small ventilation holes to facilitate air circulation.

[0048] An outer sealing partition 105 is provided on the outside of the inner breathable partition 104. The outer sealing partition 105 is connected to the outer wall of the vertical beam 103. The four outer sealing partitions 105 and the box cover 110 set on the top can completely block out external light, keeping the mouse housing space in darkness and providing the basic conditions for strong light stress experiments. Of course, when the mouse housing space needs to be under normal lighting, the outer sealing partitions 105 can be opened. Therefore, the outer sealing partitions 105 are movably connected to the vertical beam 103, and the distance between the outer sealing partitions 105 and the inner breathable partitions 104 is the thickness of the vertical beam 103.

[0049] The side of the substrate 106 is provided with a feces collection trough 108. The feces collection trough 108 is arranged at an angle and the outlet end is located outside the outer sealing partition 105, which makes it convenient for the experimenter to clean the mouse breeding space. As one embodiment, placing wood chips or plastic chips in the mouse breeding space is beneficial to cleaning and prevents the mouse excrement from directly contacting the substrate 106. When cleaning, the wood chips or plastic chips and the excrement on them can be directly replaced.

[0050] To facilitate needle prick stress experiments, a pinhole plate 107 is slidably disposed below the substrate 106. The pinhole plate 107 has multiple through-holes penetrating both the upper and lower surfaces, allowing the needle 601 to puncture the sole of the mouse's foot. Since the substrate 106 does not have corresponding through holes, a portion of the substrate 106 can be folded to expose the pinhole plate 107 for the experiment. After the experiment, the folded structure of the substrate 106 is restored. It is important to note that the pinhole plate 107 slides close to the lower surface of the substrate 106 to avoid excessive gaps between them.

[0051] Both the inner breathable partition 104 and the outer sealing partition 105 are equipped with boxes 109, and the boxes 109 are positioned correspondingly. Mice can be placed in or their feeding boxes can be changed through the boxes 109. The boxes 109 are connected to the box changing channel to drive mice into another feeding box, which is convenient for personnel to clean or disinfect.

[0052] The mouse rearing space is equipped with a feeding mechanism 20 to ensure the normal life and metabolic activities of the mice. The feeding mechanism 20 includes a water trough 201 and a feed trough 206. A water supply pipe 202 is provided above the water trough 201, through which clean water can be added to the water trough 201 to ensure the mice have enough water. A feeding channel 207 is provided above the feed trough 206, through which feed or food can be fed into the feed trough 206.

[0053] In cold environment stress experiments on mice, the temperature is often kept below zero. The water in the conventional water supply pipe 202 and water tank 201 will freeze. In order to ensure that the mice can be supplied with water normally, a heating outer layer 203 is provided on the outside of the water supply pipe 202 and the bottom of the water tank 201. A heating element is provided inside the heating outer layer 203. The heating element heats the water to ensure that there is always a supply of drinkable water. The heating element is preferably an electric heating wire.

[0054] As a preferred embodiment, the water supply pipe 202 is connected to the water tank, and an electromagnetic switch is installed on the water supply pipe 202 to control whether clean water flows, so as to realize timed and quantitative water supply and save manual operation.

[0055] In a preferred embodiment, a water temperature thermometer 204 for measuring water temperature and a water level gauge 205 for measuring water depth are embedded inside the water tank 201. The heating element, the water temperature thermometer 204, the water level gauge 205, and the electromagnetic switches on the water supply pipe 202 are all communicatively connected to the control center 80. The water temperature thermometer 204 can monitor the temperature of the clean water in the water tank 201. When the water temperature is near the freezing point, the heating element starts working, heating the water to a suitable temperature and then stopping. This process requires the coordinated operation of the water temperature thermometer 204, the heating element, and the control center 80. When the water temperature thermometer 204 detects that the water temperature is too low, it transmits the data to the control center 80. The control center 80 then instructs the heating element to start working. Once the heating element has heated the water to a suitable temperature (which can be set according to the experiment), the water temperature thermometer 204 transmits the water temperature data to the control center 80, and the control center 80 instructs the heating element to stop heating. Similarly, the water level gauge 205 is used to measure the water volume in the water tank 201. When the water depth is lower than the set warning value, the water level gauge 205 transmits the data to the control center 80. The control center 80 instructs the electromagnetic switch on the water supply pipe 202 to open and supply water. After the quantitative water supply time, the control center 80 instructs the electromagnetic switch to close again to complete the water replenishment.

[0056] Referring to Figure 7, a restraint stress experiment is conducted on mice using a restraint mechanism 30. The restraint mechanism 30 includes a binding strap 307, a slide rail 301, and a slider 302. The slider 302 is slidably mounted on the slide rail 301. The slide rail 301 includes a transverse slide rail and a longitudinal slide rail. The transverse slide rail is mounted on the inner breathable partition 104, and the slider 302 on the transverse slide rail is connected to a left sliding plate 305 and a right sliding plate 306. The longitudinal slide rail is mounted on either the left sliding plate 305 or the right sliding plate 306, and the slider 302 on the longitudinal slide rail is connected to a front sliding plate 303 and a rear sliding plate 304. The movement of the left sliding plate 305, the right sliding plate 306, the front sliding plate 303, and the rear sliding plate 304 can restrict the mouse to a narrow area, making it unable to move. This facilitates the restraint of the mouse after capture by the experimenter. At the same time, the restraint mechanism 30 can also be used to restrain the mouse and conduct foot needle prick and electric shock stress experiments to prevent the mouse from struggling.

[0057] The binding strap 307 is connected to the left sliding plate 305, the right sliding plate 306, the front sliding plate 303, and the rear sliding plate 304, and is used to fix the mouse from above. The end of the binding strap 307 is connected to a buckle 309, and the lower half of each sliding plate is provided with a buckle block 308. The buckle 309 is fastened to the buckle block 308 to fix and limit the mouse. The binding strap 307 is made of nylon, and a layer of sponge is adhered to the inside of the nylon to avoid causing physical injury to the mouse during the experiment.

[0058] In a preferred embodiment, the left sliding plate 305, the right sliding plate 306, the front sliding plate 303, and the rear sliding plate 304 are driven by push rod motors. A miniature push rod motor is provided on each of the left sliding plate 305, the right sliding plate 306, the front sliding plate 303, and the rear sliding plate 304, and the sliding plates are moved by the miniature push rod motors.

[0059] When conducting strong light stress experiments on mice, the illumination mechanism 40 is required. The illumination mechanism 40 includes a lamp tube 401 located below the box cover 110 and a light sensor 402 located outside the inner breathable partition 104. The light sensor can detect the intensity of the light emitted by the lamp tube 401, which facilitates the quantification of the experimental process. The light sensor 402 is connected to the control center 80 for communication, which facilitates the transmission of data collected by the light sensor 402 to the control center 80 for storage.

[0060] In environmental temperature change stress experiments on mice, a temperature control mechanism is needed to alter the local temperature. This mechanism includes an air conditioner 501 and a temperature sensor. The air outlet of the air conditioner 501 is located within the mouse housing space, and the temperature sensor 502 is located outside the inner breathable partition 104. Both the air conditioner 501 and the temperature sensor 502 are communicatively connected to a control center 80. The temperature sensor 502 detects the temperature within the mouse housing space and uploads the data to the control center 80. The control center 80 determines whether to continue adjusting the temperature based on the set temperature value, thereby controlling the operation of the air conditioner 501. The temperature change stress experiment includes thermal stimulation, cold stimulation, and temperature change stimuli.

[0061] Of course, when the entire device of the present invention is in a laboratory, environmental thermal stress experiments can also be conducted by adjusting the temperature inside the laboratory, without the need to use the temperature control mechanism of the device of the present invention.

[0062] Referring to Figure 8, the acupuncture mechanism 60 can perform foot acupuncture and electric shock stress experiments on mice. The acupuncture mechanism 60 includes a slide plate 602 and needles 601 disposed on the upper surface of the slide plate 602. The distribution of the needles 601 is adapted to the through-holes on the needle plate 107. When the slide plate 602 moves upward, the needle tips of the needles 601 can pass through the through-holes and act on the sole of the mouse's foot. This experiment can be conveniently completed with the assistance of the restraint mechanism 30. The slide plate 602 moves up and down via a lifting rod 603. The lifting rod 603 extends to push the slide plate 602 upward, and retracts to lower the slide plate 602. When performing electric shock experiments on mice, an electric current can be applied by connecting a conductive wire to the tail of the needle 601.

[0063] Preferably, two cameras 70 are installed on the box frame 10. Both cameras 70 are high-definition CCD cameras. The cameras 70 face the mouse breeding space to record the mice's living and experimental conditions. The cameras 70 are connected to the control center 80 to upload and save the data for later review.

[0064] The control center 80 serves as the central receiver of signals / data and is also responsible for issuing commands to ensure that each component completes the commands and coordinates with each other. The control center 80 can be programmed using a chip and embedded in a computer. It needs to receive signals / data from the water temperature thermometer 204, water level gauge 205, light sensor 402, temperature sensor 502, camera 70, and weighing sensor 90, analyze and process this data, and then issue commands to the electromagnetic switches, push rod motors, heating elements, lamp 401 switches, and air conditioner 501 switches to complete various experiments. Alternatively, a mobile client can be created to link the control center 80 to the mobile client, allowing experimenters to view the site on mobile electronic devices and remotely control the experiments through the control center 80.

[0065] The stress model establishment device of the present invention integrates the functions of multiple stress stimulus experiments. At the same time, the components or mechanisms that realize each stress function can coordinate with each other or be independent of each other, which facilitates the simultaneous experimentation of one or more stress stimuli. It is fully functional, low in manufacturing cost, and can be widely used.

[0066] The above description is merely a preferred embodiment of the present invention, and does not represent all possible forms of the present invention. The scope of protection of the present invention is not limited to such specific statements and embodiments. Various other modifications and improvements can be made based on the technical teachings disclosed in the present invention without departing from the essence of the present invention, and these modifications and improvements are still within the scope of protection of the present invention.

Claims

1. A multifunctional mouse stress model establishment device, characterized in that, include: The enclosure frame (10) and the feeding mechanism (20), restraint mechanism (30), lighting mechanism (40), temperature control mechanism, needle pricking mechanism (60) and control center (80) are provided on the enclosure frame (10); the enclosure frame (10) is composed of mutually perpendicularly connected horizontal beams (101), longitudinal beams (102) and vertical beams (103), and horizontally placed base plates (106) are connected between the vertical beams (103). The base plates (106) are provided with inner breathable partitions (104) around their perimeter. The inner breathable partitions (104) and the base plates (106) together constitute the mouse breeding space; an outer sealing partition (104) is provided on the outside of the inner breathable partitions (104). 05), a pinhole plate (107) is slidably disposed below the substrate (106); the restraint mechanism (30) is used to conduct a restraint stress experiment on mice, the light stimulation mechanism (40) is used to conduct a strong light stress experiment on mice, the temperature control mechanism is used to conduct an environmental hot and cold change stress experiment on mice, and the acupuncture mechanism (60) is used to conduct a foot acupuncture and electric shock stress experiment on mice; the feeding mechanism (20) includes a water tank (201) and a feed trough (206), the water tank (201) is connected to a water supply pipe (202), and the feed trough (206) is connected to a feeding channel (207); heating elements are provided on the outside of the water supply pipe (202) and the bottom of the water tank (201). The outer layer (203) is equipped with a heating element inside. The water tank (201) is equipped with a water temperature thermometer (204) for measuring water temperature and a water level gauge (205) for measuring water depth. The heating element, water temperature thermometer (204), water level gauge (205), and the switch on the water supply pipe (202) are respectively connected to the control center (80). The restraint mechanism (30) includes a binding strap (307), a slide rail (301), and a slider (302). The slider (302) is slidably mounted on the slide rail (301). The slide rail (301) includes a transverse slide rail and a longitudinal slide rail. The transverse slide rail is mounted on the inner breathable partition (104). On the transverse slide rail, the slider (302) is connected to the left slide plate (305) and the right slide plate (306); the longitudinal slide rail is set on the left slide plate (305) or the right slide plate (306), and the slider (302) on the longitudinal slide rail is connected to the front slide plate (303) and the rear slide plate (304); the binding strap (307) is connected to the left slide plate (305), the right slide plate (306), the front slide plate (303) and the rear slide plate (304), and the end of the binding strap (307) is connected to the buckle (309). The lower half of the slide plate is provided with a buckle block (308), and the buckle (309) is fastened to the buckle block (308).

2. The multifunctional mouse stress model establishment device according to claim 1, characterized in that, The lighting mechanism (40) includes a lamp tube (401) disposed below the cover (110) and a light sensor (402) disposed outside the inner breathable partition (104), the light sensor (402) being communicatively connected to the control center (80).

3. The multifunctional mouse stress model establishment device according to claim 1, characterized in that, The temperature control mechanism includes an air conditioner (501) and a temperature sensor (502). The air outlet of the air conditioner (501) is located in the mouse breeding space, and the temperature sensor (502) is located outside the inner breathable partition (104). The air conditioner (501) and the temperature sensor (502) are respectively connected to the control center (80) for communication.

4. The multifunctional mouse stress model establishment device according to claim 1, characterized in that, The needle-punching mechanism (60) includes a slide plate (602) and needles (601) disposed on the upper surface of the slide plate (602). The distribution of the needles (601) is adapted to the through-holes on the needle plate (107). The slide plate (602) is raised and lowered by a lifting rod (603).

5. The multifunctional mouse stress model establishment device according to claim 1, characterized in that, The bottom of each vertical beam (103) is located on a weight transmitter (901), which is connected to a weighing sensor (90), and the weighing sensor (90) is communicatively connected to a control center (80).

6. The multifunctional mouse stress model establishment device according to claim 1, characterized in that, The substrate (106) is provided with a fecal collection trough (108) on its side. The fecal collection trough (108) is arranged at an angle and its outlet is located outside the outer sealing partition (105).

7. The multifunctional mouse stress model establishment device according to any one of claims 1 to 6, characterized in that, Multiple cameras (70) are installed on the box frame (10), with the camera (70) facing the mouse breeding space, and the camera (70) is communicatively connected to the control center (80).

8. The multifunctional mouse stress model establishment device according to claim 7, characterized in that, Both the inner breathable partition (104) and the outer sealing partition (105) are provided with a box door (109), and the box door (109) is movably connected to a box changing channel.

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