Anticorrosive and antibacterial composition as well as preparation method and application thereof

Abstract

The invention belongs to the technical field of cosmetic raw materials, and particularly relates to an anticorrosive and antibacterial composition as well as a preparation method and application thereof. The antiseptic and antibacterial composition is prepared from the following components in percentage by mass: 10 to 30 percent of antibacterial lipopeptide, 0.1 to 1 percent of lysozyme, 0.01 to 0.05 percent of capryloyl hydroximic acid, 0.2 to 0.6 percent of p-hydroxyacetophenone and the balance of water. The preparation method comprises the following steps: screening and extracting a natural compound-antibacterial lipopeptide with antibacterial and preservative activity and a natural antibacterial substance-lysozyme from a natural microorganism source, combining two chemical preservatives, namely capryloyl hydroxamic acid and p-hydroxyacetophenone, and compounding to obtain a novel preservative and antibacterial composition; the compound form can significantly reduce the usage amount of chemical preservatives so as to ensure the safety and low toxicity of the composition, and all the components cooperate with one another to play a synergistic effect so as to further improve the antiseptic and antibacterial properties of the composition.

Description

Technical Field

[0001] The present invention belongs to the technical field of cosmetic raw materials, and particularly relates to an anti-corrosion and antibacterial composition, a preparation method thereof and an application thereof. Background Art

[0002] The cosmetic industry has long used chemically synthesized preservatives to extend the shelf life of products. However, these chemically synthesized preservatives have some deficiencies, such as irritation, allergy, inflammation and other problems to human skin. In order to improve the safety and efficacy of cosmetics, people have begun to search for safe and highly effective antibacterial active substances from natural products to replace chemically synthesized preservatives.

[0003] There are some natural compounds with antibacterial and anti-corrosion activities in natural microbial sources, such as antibacterial lipopeptides and natural antibacterial substance lysozyme. These natural compounds not only have good antibacterial activity, but also have no toxic side effects on the human body. By screening and extracting these compounds from natural microorganisms and compounding them with other preservatives to form a new type of preservative composition, the dosage of chemically synthesized preservatives can be effectively reduced, thereby reducing their irritation to the human body.

[0004] Therefore, developing a new type of preservative composition, using natural compounds such as antibacterial lipopeptides and lysozyme from natural microorganisms and compounding them with other preservatives, can not only improve the anti-corrosion effect, but also reduce the usage amount of chemically synthesized preservatives and reduce their irritation to human skin, thereby improving the safety and efficacy of cosmetics, which is of great significance to the development of the cosmetic industry. Summary of the Invention

[0005] The purpose of the present invention is to overcome the deficiencies of the above-mentioned prior art and provide an anti-corrosion and antibacterial composition, a preparation method thereof and an application thereof that can effectively improve the anti-corrosion effect and reduce irritation.

[0006] To achieve the above purpose, the technical solutions adopted by the present invention include:

[0007] In the first aspect, the present invention provides an anti-corrosion and antibacterial composition, and the anti-corrosion and antibacterial composition includes the following components in mass percentage: 10-30% of antibacterial lipopeptide, 0.1-1% of lysozyme, 0.01-0.05% of octanoyl hydroxamic acid, 0.2-0.6% of p-hydroxyacetophenone and the balance of water.

[0008] The present invention mainly screens and extracts natural compounds with antibacterial and antiseptic activities, namely antibacterial lipopeptides and natural antibacterial substances, lysozyme, from natural microbial sources, and combines them with two chemical preservatives, octanoyl hydroxamic acid and p-hydroxyacetophenone, to form a novel antiseptic and antibacterial composition. This form of compounding can effectively reduce the dosage of chemical preservatives, achieve the safety and low toxicity of the antiseptic composition, and the components cooperate with each other to synergistically improve the antibacterial and antiseptic performance of the composition. Through research, it is found that the antiseptic and antibacterial composition has inhibitory activity against fungi such as Candida albicans and Aspergillus niger; it also has strong inhibitory activity against Gram-positive bacteria such as Staphylococcus aureus and Propionibacterium acnes, as well as Gram-negative phytopathogenic bacteria such as Escherichia coli and Pseudomonas aeruginosa, thus being able to exert a broad-spectrum and highly efficient antibacterial effect.

[0009] Moreover, the inventors explored the dosages of each component and found that when the dosage ratios of the antibacterial lipopeptides, lysozyme and the other two components are within the above-mentioned preferred ranges, the components interact with each other to exert an ideal synergistic effect, thereby effectively enhancing the antibacterial and antiseptic effects of the composition. When the dosage ratios of the four components exceed the defined ranges, the synergistic effect among the components is not obvious or even antagonistic, which in turn leads to a decrease in the antibacterial effect of the composition.

[0010] Preferably, the antiseptic and antibacterial composition comprises the following components in mass percentage: 15-25% of antibacterial lipopeptides, 0.3-0.7% of lysozyme, 0.01-0.03% of octanoyl hydroxamic acid, 0.3-0.5% of p-hydroxyacetophenone, and the balance of water.

[0011] Preferably, the antiseptic and antibacterial composition comprises the following components in mass percentage: 20% of antibacterial lipopeptides, 0.4% of lysozyme, 0.02% of octanoyl hydroxamic acid, 0.3% of p-hydroxyacetophenone, and the balance of water.

[0012] Through experimental exploration, it is found that when the components of the present invention are formulated with the above-defined optimal dosage ratios, the prepared antiseptic and antibacterial composition has the best antibacterial effect and good safety, and will not cause irritating reactions to the skin.

[0013] Preferably, the preparation method of the antibacterial lipopeptides comprises the following steps:

[0014] S1. Inoculate Bacillus subtilis into a liquid medium and culture to prepare a seed solution;

[0015] S2. Inoculate the seed solution into a liquid medium for culture and fermentation to obtain a fermentation broth;

[0016] S3. Centrifuge the fermentation broth, take the fermentation supernatant, adjust the pH value, centrifuge again, discard the supernatant and take the precipitate, add methanol to the precipitate, vortex and shake, ultrasonicate, extract, centrifuge, and take the supernatant and filter it to obtain the antibacterial lipopeptides.

[0017] Preferably, in the step S2, the inoculation amount of the seed liquid is 2-5%, and the specific fermentation conditions are: the temperature is 30-37 °C, the time is 48-96 h, and the rotation speed is 160-220 rpm.

[0018] Preferably, in the step S3, the centrifugation conditions are: centrifuging at 7000-9000 rpm for 10-20 min; the specific method for adjusting the pH value is: adjusting the pH to 1.6-2.4 with 5-7 mol / L hydrochloric acid.

[0019] Preferably, in the step S3, the time of vortex oscillation is 8-12 min, the time of ultrasonic treatment is 10-20 min, and the extraction time is 4-8 h.

[0020] The present invention uses Bacillus subtilis to ferment and culture under the above preparation process to obtain antibacterial lipopeptide, which can effectively improve the yield, stability and antibacterial activity of antibacterial lipopeptide. Therefore, when it is compounded with lysozyme, octanoyl hydroxamic acid and p-hydroxyacetophenone, the antibacterial and antiseptic effect of the composition can be further improved.

[0021] In the second aspect, the present invention provides a preparation method of the antiseptic and antibacterial composition, comprising the following steps:

[0022] Mix all components and stir evenly to obtain the antiseptic and antibacterial composition.

[0023] In the third aspect, the present invention provides an application of the antiseptic and antibacterial composition in cosmetics.

[0024] Preferably, the mass percentage content of the antiseptic and antibacterial composition in cosmetics is 10-25%.

[0025] Compared with the prior art, the beneficial effects of the present invention are:

[0026] (1) The present invention screens and extracts a natural compound with antibacterial and antiseptic activity - antibacterial lipopeptide and a natural antibacterial substance - lysozyme from natural microbial sources, combines two chemical preservatives, octanoyl hydroxamic acid and p-hydroxyacetophenone, and formulates a novel antiseptic and antibacterial composition. This compounding form can significantly reduce the usage amount of chemical preservatives, thereby ensuring the safety and low toxicity of the composition, and each component cooperates with each other to play a synergistic effect, so that the composition has a broader spectrum and more efficient antibacterial effect;

[0027] (2) The present invention has conducted a microbial anti-corrosion challenge test, verifying that the anti-corrosion and antibacterial composition can replace traditional organic synthetic preservatives, and its anti-corrosion effect is not inferior to that of traditional preservatives. Moreover, through the chicken embryo chorioallantoic membrane test, it is verified that the novel anti-corrosion and antibacterial composition has little irritation to the skin, thus providing an effective solution for achieving low-toxicity, safety, and high-efficiency anti-corrosion and antibacterial in the cosmetics field. BRIEF DESCRIPTION OF THE DRAWINGS

[0028] Figure 1 It is a finished product appearance diagram of the anti-corrosion and antibacterial composition described in Example 1. DETAILED DESCRIPTION OF THE EMBODIMENTS

[0029] To better illustrate the purpose, technical solution, and advantages of the present invention, the present invention will be further described below in conjunction with specific embodiments. Those skilled in the art should understand that the specific embodiments described herein are only used to explain the present invention and are not used to limit the present invention.

[0030] Unless otherwise specified, all raw materials involved in the following examples and comparative examples are commercially available general materials.

[0031] The lysozyme described in the present invention is derived from egg white, and its lysozyme activity is 1000 U / mL.

[0032] Examples 1 - 5

[0033] Examples 1 - 5 of the present invention provide an anti-corrosion and antibacterial composition, and the specific formula is shown in Table 1. The preparation method of the composition includes the following steps:

[0034] Mix lysozyme, antibacterial lipopeptide, octanoyl hydroxamic acid, and p-hydroxyacetophenone with water and stir evenly to obtain the anti-corrosion and antibacterial composition.

[0035] Among them, the preparation method of the antibacterial lipopeptide is as follows:

[0036] (1) Preparation of Bacillus subtilis seed liquid:

[0037] Pick a single colony of Bacillus subtilis on the LB solid medium with an inoculation loop and transfer it to the LB liquid medium. Culture it at 37°C and 220 rpm for 16 - 18 h to prepare the seed liquid;

[0038] (2) Fermentation of Bacillus subtilis:

[0039] Inoculate the seed liquid into the liquid LB medium at a strain ratio of 3% (v / v). The fermentation temperature is 35°C, the fermentation time is 72 h, and the shaker speed is 180 rpm;

[0040] (3) Preparation of Bacillus subtilis antibacterial lipopeptide:

[0041] The fermentation broth was centrifuged at 8000 rpm for 15 min to obtain the fermentation supernatant. The pH of the fermentation supernatant was adjusted to 2.0 with 6 mol / L hydrochloric acid and placed in a refrigerator at 4°C overnight. The fermentation supernatant was centrifuged at 8000 rpm for 15 min, the supernatant was discarded, and the precipitate was left. After adding methanol, it was vortexed for 10 min, sonicated for 15 min, extracted for 4 - 8 h, centrifuged again, and the supernatant was filtered through a 0.22 μm filter membrane to obtain the antibacterial lipopeptide produced by Bacillus subtilis.

[0042] Table 1 Formulation table of the anti - corrosion and antibacterial composition described in Examples 1 - 5 (mass percentage)

[0043] Component Example 1 Example 2 Example 3 Example 4 Example 5 Lysozyme 0.4 0.1 1 0.3 0.7 Antibacterial lipopeptide 20 10 30 15 25 Octanoyl hydroxamic acid 0.02 0.01 0.05 0.01 0.03 p-Hydroxyacetophenone 0.3 0.2 0.6 0.3 0.5 Water Balance Balance Balance Balance Balance

[0044] Comparative Examples 1 - 10

[0045] Comparative Examples 1 - 10 provided an anti - corrosion and antibacterial composition, and the specific formulation is shown in Table 2. The preparation method of the composition is the same as that of the examples, and the difference lies only in the formulation.

[0046] Table 2 Formulation table of the anti - corrosion and antibacterial composition described in Comparative Examples 1 - 10 (mass percentage)

[0047]

[0048] Comparative Example 11

[0049] This comparative example provided an anti - corrosion and antibacterial composition, and the difference from Example 1 was only that: an equal amount of phenoxyethanol was used to replace p - hydroxyacetophenone, and the other components and their contents were the same as those in Example 1.

[0050] Comparative Example 12

[0051] This comparative example provided an anti - corrosion and antibacterial composition, and the difference from Example 1 was only that: an equal amount of nisin was used to replace the antibacterial lipopeptide, and the other components and their contents were the same as those in Example 1.

[0052] Effect Example 1 Determination of the inhibition zone

[0053] (1) Test samples: The compositions prepared in Examples 1 - 5 and Comparative Examples 1 - 12 were used as test samples to measure the inhibition zone to evaluate the antibacterial activity of the samples;

[0054] (2) Preparation of bacterial suspensions: Scrape the strains of Staphylococcus aureus (CICC 10384), Escherichia coli (CICC 24176), Pseudomonas aeruginosa (CICC 24649), Candida albicans (CICC 1965), and Propionibacterium acnes (ATCC11827) from the activated strain test tubes respectively, and spread them on the corresponding solid media. The bacteria are cultured at 30 °C for 24 hours, and the fungi are cultured at 25 °C for 48 hours. Elute the strains with sterile normal saline to prepare bacterial suspensions with different concentrations;

[0055] (3) Preparation of filter paper discs: Select qualitative filter paper with good water absorption, and use a punching machine to punch out circular filter paper discs with a diameter of 6 mm, and sterilize them by high-pressure steam for later use;

[0056] (4) Determination of inhibition zones: Adopt the filter paper diffusion method. Use a punching machine to make circular filter paper discs with a diameter of 5 mm from the filter paper, place them in a clean small beaker, sterilize them at 121 °C for 20 min and then dry them. Then, use a pipette to transfer 5 μL of the test sample and drop it on the filter paper discs to fully moisten them. Pour the sterilized nutrient agar or PDA medium into the petri dishes respectively. After cooling and solidifying, pipette 0.1 mL of the above-mentioned bacterial or fungal suspensions and spread them evenly on the plates. Then, use sterile forceps to pick up the circular filter paper discs soaked with each component and stick them on the bacteria-containing plates. Stick 4 discs equidistantly on each plate, and use 1 filter paper disc soaked with sterile water as a blank control. Place the corresponding petri dishes in an inverted position in a constant temperature incubator at 30 °C for 24 h (for bacteria) or at 25 °C for 48 h (for fungi), measure the diameters of the inhibition zones of each strain under different test samples, with 3 replicates for each sample, and take the average value. The specific results are shown in Table 3.

[0057] Table 3 Results of the diameters of inhibition zones (mm)

[0058]

[0059]

[0060] As can be seen from the results in Table 3, the antibacterial effects of the anti-corrosion and antibacterial compositions prepared in Examples 1-5 of the present invention are significantly better than those of single components, and they have broad-spectrum inhibitory ability against Gram-positive bacteria, Gram-negative bacteria, and fungi, and are applicable to a variety of application scenarios. In particular, the anti-corrosion and antibacterial composition described in Example 1 has the best broad-spectrum antibacterial effect.

[0061] When only a single component of lysozyme, antimicrobial lipopeptide, octanoyl hydroxamic acid, and p-hydroxyacetophenone is used in Comparative Examples 1-4, and when any one or two of the above four components are missing in Comparative Examples 5-8, the broad-spectrum antibacterial effect of the prepared antiseptic and antibacterial composition is significantly reduced compared with that of the Examples. In particular, the antibacterial effect against Pseudomonas aeruginosa and Propionibacterium acnes decreases significantly. When the dosage ratios of the four components in Comparative Examples 9-10 exceed the scope defined in the present invention, the antibacterial effect of the composition also weakens. The above results show that the four components selected in the composition of the present invention can exert an ideal synergistic effect within a specific dosage range, thereby enhancing the antibacterial effect of the composition, and improving the limitation of poor antibacterial effects of the single component against Gram-negative bacteria such as Pseudomonas aeruginosa and Gram-positive bacteria such as Propionibacterium acnes, broadening the antibacterial spectrum and application range of the composition, and thus providing a more comprehensive antibacterial effect. When the components with antibacterial effects are used to replace the components selected in the present invention in Comparative Examples 11-12 respectively, the antibacterial effect of the prepared composition also becomes worse, indicating that the four components of the present invention can exert a unique synergistic effect, and this effect cannot be achieved by conventionally replacing other components.

[0062] Effect Example 2 Determination of Minimum Inhibitory Concentration (MIC)

[0063] In this effect example, the antiseptic and antibacterial composition prepared in Example 1 was used as a test sample, and its minimum inhibitory concentration was determined. The specific method is as follows:

[0064] (1) Prepare nutrient broth medium;

[0065] (2) MIC determination: Dilute the test sample into five concentration gradients of 5%, 10%, 15%, 20%, and 25%, add the bacterial liquid of the corresponding strain respectively, and observe the growth situation to judge the MIC value after culturing at 36 °C (for bacteria) or 28 °C (for fungi) for a certain period of time. The specific results are shown in Table 4.

[0066] Table 4

[0067]

[0068]

[0069] As can be seen from the results in Table 4, the antiseptic and antibacterial composition of the present invention shows good antibacterial effects against the tested bacteria and fungi, and its minimum inhibitory concentration (MIC) for all tested microorganisms is 10%. This result indicates that the composition has high antibacterial performance in cosmetics and can effectively inhibit the growth of common cosmetic contaminating bacteria.

[0070] Effect Example 3 Microbiological Antiseptic Challenge Test

[0071] This efficacy example uses the anti-corrosion and antibacterial composition prepared in Example 1 as the test sample, and conducts a 28-day microbial anti-corrosion challenge test on it. The test refers to the T-GDCDC 010-2019 Cosmetics Anti-corrosion Challenge Test Method. The specific method is as follows:

[0072] (1) Weigh a certain amount of the test sample and add a mixed bacterial suspension according to the proportion, requiring the initial bacterial inoculation concentration in the sample to reach 10 6 cfu / g; the initial inoculation concentration of yeast and mold reaches 10 5 cfu / g; the standard strains used are: Staphylococcus aureus (CICC 10384), Escherichia coli (CICC 24176), Pseudomonas aeruginosa (CICC 24649), Candida albicans (CICC 1965), Aspergillus niger (CICC 2487);

[0073] (2) Thoroughly mix the 10% concentration test sample with the bacterial solution, seal the sample bottle mouth with sealing tape, and store it in an incubator at 25°C;

[0074] (3) Take samples on the 7th, 14th, 21st, and 28th days after inoculation to detect the microbial content, and calculate the logarithmic reduction value of the colony count.

[0075] (4) Judge whether the anti-corrosion effect meets the standard according to the logarithmic reduction value. Table 5 shows the background microorganism inspection results, Table 6 shows the anti-corrosion challenge test results of the test sample, and Table 7 shows the anti-corrosion effect evaluation criteria.

[0076] Table 5 Background Microorganism Inspection Results

[0077] Inspection item Unit Inspection result Limit value Total number of colonies CFU / g <10 ≤100 Total number of molds and yeasts CFU / g <10 ≤100

[0078] Table 6 Anti-corrosion Challenge Test Results

[0079]

[0080] Table 7 Anti-corrosion Effect Evaluation Criteria

[0081]

[0082]

[0083] From the results of Tables 5-7, it can be seen that the anti-corrosion challenge test of the anti-corrosion and antibacterial composition described in the present invention passes and meets the requirements of Standard I, can replace traditional organic synthetic preservatives, and the anti-corrosion effect is not inferior to traditional preservatives.

[0084] Effect Example 4 Human Skin Patch Test

[0085] This efficacy example uses the anti-corrosion and antibacterial composition (concentration 10%) prepared in Examples 1-5 as the test sample, and conducts a human skin patch test on it to evaluate the skin safety of the composition for humans. The specific method is as follows:

[0086] A human skin patch test was conducted with 50 volunteers as the experimental subjects. A qualified patch tester with an area <50 mm 2 and a depth of 1 mm was selected. A syringe was used to draw 0.025 mL of the test sample and placed it in the medicine chamber of the patch tester. The control hole was a blank control (without placing any substance). The patch tester with the test sample was longitudinally affixed to the normal skin on the flexor side of the left forearm of the volunteer from bottom to top with a low-sensitization tape, and the palm was gently pressed to evenly apply the test sample on the skin for 24 h. The skin reactions were observed at 30 min (after the indentation disappeared), 24 h, and 48 h after removing the test sample patch tester, and the results were recorded according to the skin reaction grading standard in the "Technical Specifications for Cosmetics Safety" (2015 edition).

[0087] The results of the human skin patch test showed that 0 out of 50 people had a positive reaction. According to the regulations in the "Technical Specifications for Cosmetics Safety" (2015 edition), this test sample does not cause skin adverse reactions, and the sample is safe and non-irritating.

[0088] Efficacy Example 5 Chicken Embryo Chorioallantoic Membrane Test

[0089] This efficacy example uses the anti-corrosion and antibacterial composition (concentration 10%) prepared in Examples 1-5 as the test sample, and conducts a chicken embryo chorioallantoic membrane test on it to evaluate the irritation of the composition to humans. The specific test method is as follows:

[0090] (1) Chicken embryo strain: Fertilized chicken embryos of varieties such as White Leghorn chicken were selected, with a chicken embryo weight of 50 g - 60 g. SPF chicken embryos were preferably used, and the quality of the chicken embryos met the requirements of relevant standards;

[0091] (2) Incubation conditions: Room temperature 20 - 25°C, incubation temperature 37.5°C ± 0.5°C, relative humidity 55 - 70%, turntable frequency 3 times / h - 6 times / h. Chicken embryos at 9 months of age do not need to be rotated during incubation;

[0092] (3) CAM preparation:

[0093] 1. On the 9th day of incubation, the chicken embryos are candled to check, and the position of the air chamber is marked on the eggshell surface; the part of the eggshell with the mark is peeled off with a dental serrated curved forceps to expose the white egg membrane, and care should be taken not to damage the integrity of the egg membrane during the operation. 1-2 mL of sterile normal saline is dropped with a pipette to moisten the egg membrane. At this time, the next step can be carried out immediately. Otherwise, the chicken embryos should be placed in an incubator or under a lamp (to prevent the temperature of the chicken embryos from dropping), and the placement time should not exceed 20 minutes; the sterile normal saline is poured out, and the inner membrane is carefully removed with forceps to ensure that the vascular membrane is not damaged. At this time, the structure of the vascular system should be observed again, and a judgment should be made on its integrity and suitability for the test;

[0094] 2. Place a sterilized Teflon ring on the position where the blood vessels on the CAM membrane are intact and clear, drop 40 μL of the test solution, start timing immediately, observe the reaction situation, and record the time when each toxic effect appears within 5 minutes of the action (take 0.3 mL of the above transparent liquid and directly drop it on the surface of the CAM, observe the reaction situation of the CAM, and record the time when each toxic effect appears within 5 minutes of the action).

[0095] (4) Irritation Scoring Method (IS):

[0096] For the test using the reaction time method, the irritation score (IS) is calculated using Equation (1), and the result is retained to two decimal places:

[0097]

[0098] In the formula: sec H (hemorrhage time) is the average time when bleeding starts to occur observed on the CAM membrane, and the unit is seconds (S);

[0099] sec L (vessel lysis time) is the average time when vessel lysis starts to occur observed on the CAM membrane, and the unit is seconds (S);

[0100] sec C (coagulation time) is the average time when coagulation starts to appear observed on the CAM membrane, and the unit is seconds (S).

[0101] Classify the eye irritation of the test substance according to the calculated IS value in Table 8.

[0102] Table 8 Evaluation of the results of the irritation scoring method

[0103] Stimulation score Stimulation classification IS < 1 Non-irritating 1 ≤ IS < 5 Mild irritation 5 ≤ IS < 10 Moderate irritation IS ≥ 10 Severe irritation / corrosion

[0104] After testing, the anti-corrosion and antibacterial composition prepared by the present invention showed no irritation in the chicken embryo chorioallantoic membrane test, and the irritation score was 0. This result indicates that the composition has good safety in cosmetics and will not cause irritation to the skin, further verifying its safety in cosmetic applications.

[0105] Finally, it should be noted that the above embodiments are used to illustrate the technical solutions of the present invention rather than to limit the protection scope of the present invention. Although the present invention has been described in detail with reference to the preferred embodiments, those of ordinary skill in the art should understand that the technical solutions of the present invention can be modified or equivalently replaced without departing from the essence and scope of the technical solutions of the present invention.

Claims

1. An antiseptic and antibacterial composition, characterized in that: The antiseptic and antibacterial composition comprises the following components in percentage by mass: 10-30% of antibacterial lipopeptide, 0.1-1% of lysozyme, 0.01-0.05% of octanoylhydroxamic acid, 0.2-0.6% of p-hydroxyacetophenone and the balance of water.

2. The antiseptic and antibacterial composition according to claim 1, characterized in that: The antiseptic and antibacterial composition comprises the following components in percentage by mass: 15-25% of antibacterial lipopeptide, 0.3-0.7% of lysozyme, 0.01-0.03% of octanoylhydroxamic acid, 0.3-0.5% of p-hydroxyacetophenone and the balance of water.

3. The antiseptic and antibacterial composition according to claim 1, characterized in that: The antiseptic and antibacterial composition comprises the following components in percentage by mass: 20% of antibacterial lipopeptide, 0.4% of lysozyme, 0.02% of octanoylhydroxamic acid, 0.3% of p-hydroxyacetophenone and the balance of water.

4. The antiseptic and antibacterial composition according to any one of claims 1 to 3, characterized in that: The preparation method of the antimicrobial lipopeptide comprises the following steps: S1. inoculating Bacillus subtilis into a liquid culture medium and culturing to prepare a seed solution; S2, inoculating the seed liquid into a liquid culture medium for culturing and fermenting to obtain a fermentation liquid; S3, centrifuging the fermentation liquid, taking the fermentation supernatant, adjusting the pH value, centrifuging, discarding the supernatant and taking the precipitate, adding methanol to the precipitate, vortexing, ultrasonicating, extracting, centrifuging, taking the supernatant and filtering, and thus obtaining the antimicrobial lipopeptide.

5. The antiseptic and antibacterial composition according to claim 4, characterized in that: In step S2, the inoculation amount of the seed liquid is 2-5%, and the specific conditions of the fermentation are: temperature of 30-37° C., time of 48-96 h, and rotation speed of 160-220 rpm.

6. The antiseptic and antibacterial composition according to claim 4, characterized in that: In step S3, the centrifugation conditions are: centrifugation at 7000-9000 rpm for 10-20 min; the specific method for adjusting the pH value is: using 5-7 mol / L hydrochloric acid to adjust the pH to 1.6-2.

4.

7. The antiseptic and antibacterial composition according to claim 4, characterized in that: In step S3, the vortexing time is 8-12 minutes, the ultrasonication time is 10-20 minutes, and the extraction time is 4-8 hours.

8. The method for preparing the antiseptic and antibacterial composition according to any one of claims 1 to 3, characterized in that: The following steps are involved: All components are mixed and stirred evenly to obtain the antiseptic and antibacterial composition.

9. Use of the antiseptic and antibacterial composition according to any one of claims 1 to 7 in cosmetics.

10. The use according to claim 9, characterized in that The mass percentage of the antiseptic and antibacterial composition in the cosmetics is 10-25%.

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