Application of sargassum pallidum polysaccharide in preparation of whitening cosmetics

Abstract

The invention discloses application of sargassum pallidum polysaccharide in preparation of whitening cosmetics, and belongs to the technical field of marine organisms. The sargassum pallidum polysaccharide reduces melanogenesis by inhibiting tyrosinase activity and realizes skin whitening. The polysaccharide is powdery, the content of the polysaccharide is greater than or equal to 50% in terms of glucose, the weight loss on drying is less than or equal to 1%, the residue on ignition is less than or equal to 0.2%, and the polysaccharide meets the microbial safety standard (total bacterial count lt; 1000 CFU / g, mould and saccharomycetes 1t; the pathogenic bacteria cannot be detected). Experiments prove that the half inhibitory mass concentration (IC50) of the compound on tyrosinase is 4.27 mg / mL, which indicates that the compound has remarkable enzymatic activity inhibition ability. Compared with traditional whitening components, the polysaccharide has the advantages of being high in stability, excellent in safety, low in irritation and the like, and a natural, mild and efficient active component solution is provided for whitening products.

Description

Technical Field

[0001] The present invention relates to the field of marine biotechnology, and particularly to the application of Sargassum pallidum polysaccharide in the preparation of whitening cosmetics. Background Art

[0002] In the field of cosmetics, the realization of whitening efficacy highly depends on the effective regulation of the activity of tyrosinase, a key enzyme in melanin synthesis. Although current mainstream whitening ingredients such as hydroquinone and kojic acid have certain inhibitory effects, they face bottlenecks such as safety controversies, poor chemical stability, or low transdermal absorption efficiency. For example, chemically synthesized ingredients are prone to cause skin irritation or photosensitivity reactions, while some natural extracts are difficult to penetrate the stratum corneum to exert their activity due to their large molecular weight or complex structure, limiting their practical application value.

[0003] In recent years, marine algal resources have attracted attention due to their rich bioactive substances and environmentally friendly characteristics. Sargassum pallidum, a wild alga widely distributed along the coast of China, has been applied in the food and pharmaceutical fields with its active ingredients (such as polysaccharides and polypeptides), but its development in the cosmetics field is still in the exploratory stage. In the existing technology, the research on Sargassum pallidum polysaccharide mostly focuses on its immunomodulatory or antioxidant functions, and its potential in the whitening field has not been fully explored.

[0004] In view of the above technical problems, the present invention first proposes to use high-purity Sargassum pallidum polysaccharide as a tyrosinase inhibitor in whitening cosmetics. It provides an innovative direction for the development of safe, stable and efficient marine-derived whitening ingredients, and promotes the high-value utilization of Sargassum pallidum resources in the cosmetics field. Summary of the Invention

[0005] The technical problem to be solved by the present invention is to provide the application of Sargassum pallidum polysaccharide in the preparation of whitening cosmetics in view of the deficiencies of the prior art.

[0006] To achieve the purpose of the present invention, the following technical means are specifically adopted:

[0007] The application of Sargassum pallidum polysaccharide in the preparation of whitening cosmetics.

[0008] Sargassum pallidum polysaccharide exerts a whitening effect by inhibiting tyrosinase.

[0009] The Sargassum pallidum polysaccharide described in the present invention is in powder form, brown, with a polysaccharide content (calculated as glucose) ≥ 50%, loss on drying ≤ 1%, incineration residue ≤ 0.2%, heavy metals ≤ 10 ppm, pesticide residues ≤ 2 ppm, total number of bacteria < 1000 CFU / g, molds and yeasts < 100 CFU / g, Salmonella not detected, and Escherichia coli not detected.

[0010] Advantageous Effects

[0011] Advantages of the present invention:

[0012] The present invention discloses that Sargassum polysaccharide can significantly inhibit the activity of tyrosinase. Compared with traditional whitening ingredients, Sargassum polysaccharide has a mild effect and is not likely to cause skin discomfort, providing consumers with a safer and more efficient whitening option. Description of the drawings

[0013] Figure 1 It is the inhibition curve of kojic acid on tyrosinase;

[0014] Figure 2 It is the inhibition curve of Sargassum polysaccharide on tyrosinase. Detailed implementation manners

[0015] The technical solution of the present invention will be clearly and completely described below in conjunction with the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all of the embodiments. The description of at least one exemplary embodiment is actually only illustrative and in no way limits the present invention and its application or use. All other embodiments obtained by those of ordinary skill in the art based on the embodiments of the present invention without creative efforts belong to the scope of protection of the present invention.

[0016] Description of the Sargassum polysaccharide used in the present invention: The manufacturer is Shaanxi Mixian'er Biotechnology Co., Ltd., the product batch number is 2024.08.21, the polysaccharide content (calculated as glucose) is 50.1%, the loss on drying is 0.54%, the incineration residue is 0.12%, the heavy metals ≤ 10 ppm, the pesticide residues ≤ 2 ppm, the total number of bacteria < 1000 CFU / g, the molds and yeasts < 100 CFU / g, Salmonella was not detected, and Escherichia coli was not detected.

[0017] Example 1

[0018] Experiment on the inhibitory effect of Sargassum polysaccharide on tyrosinase:

[0019] The dopa staining method was used to determine the effect of the test sample on the activity of tyrosinase.

[0020] The total volume of the reaction system was 200 μL. 50 μL of PBS phosphate buffer with pH 6.8, 50 μL of tyrosinase solution at 120 U / mL, and 50 μL of samples or reference substances at different concentrations were added in sequence. After preheating at 37 °C for 10 min, 50 μL of L-dopa (0.4 mg / mL, prepared with PBS phosphate buffer at pH 6.8) was added and reacted for 10 min. The absorbance was measured at 475 nm with an enzyme-labeling instrument, and the inhibition rate of tyrosinase was calculated. In addition, a negative control group, a background group, and a blank group were set up. The details of each reaction system are shown in Table 1 below. The IC was calculated through the relationship between the enzyme activity inhibition rate and the concentration curve.50 Value

[0021] Table 1 Tyrosinase inhibition reaction system for different groups (unit: μL)

[0022] Group Control group Background group Sample group Blank group PBS 100 150 50 100 Sample, kojic acid - - 50 50 Tyrosinase 50 - 50 - L-DOPA 50 50 50 50

[0023] The calculation formula for tyrosinase inhibition rate is: Inhibition rate (%) = [1 - (A 样品 - A 空白 ) / (A 对照 - A 背景 )] × 100%

[0024] Where: A 对照 is the reaction absorbance value without inhibitor; A 背 is the reaction absorbance value without inhibitor and tyrosinase; A 样品 is the reaction absorbance value of the sample or positive drug; A 空白 is the reaction absorbance value without tyrosinase.

[0025] Experimental results:

[0026] Table 2 Effects of Sargassum polysaccharide and kojic acid on tyrosinase activity

[0027] Sample <![CDATA[Half inhibitory mass concentration (IC 50 ) / (mg / mL)]]> Sargassum polysaccharide 4.27 Kojic acid 0.027

[0028] The above results show that Sargassum polysaccharide can inhibit tyrosinase activity.

[0029] The above is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principle of the present invention shall be included within the protection scope of the present invention.

Claims

1. An application of Artemisia seagrass polysaccharide in the preparation of whitening cosmetics, characterized in that: The quality indicators of the sea wormwood polysaccharide include: polysaccharide content calculated as glucose ≥50%, drying loss ≤1%, ignition residue ≤0.2%, total heavy metal content ≤10ppm, pesticide residue ≤2ppm, total bacteria count <1000CFU / g, mold and yeast <100CFU / g, and no detection of Salmonella and Escherichia coli; the application achieves whitening effect by inhibiting tyrosinase activity and reducing melanin synthesis.

2. The use according to claim 1, characterized in that: The half inhibition concentration (IC50) of the sea wormwood polysaccharide on tyrosinase is 4.0-4.5 mg / mL.

3. The use according to claim 1, characterized in that: The sea wormwood polysaccharide is in the form of brown powder and meets the microbial safety standards for cosmetic raw materials.

4. The use according to claim 1, characterized in that: The sargassum polysaccharide is derived from sargassum pallidum.

5. A whitening cosmetic, characterized in that: The cosmetic comprises the Artemisia seagrass polysaccharide according to any one of claims 1 to 4 as an active ingredient, and the amount of the Artemisia seagrass polysaccharide added to the cosmetic is 0.5% to 10% (mass percentage).

6. The whitening cosmetic according to claim 5, characterized in that: The dosage form of the cosmetic is emulsion, essence or facial mask.

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