Application of Boswellia carteri essential oil in prevention and treatment of aquatic diseases

Abstract

The invention belongs to the technical field of application of natural extracts, and particularly discloses application of Boswellia amoena essential oil in prevention and treatment of aquatic diseases, in particular application of Boswellia amoena essential oil in inhibition of shewanella algal and vibrio alginolyticus. According to the application, various plant essential oils are tested, the bacteriostatic activity of the plant essential oils on two marine vibrios, i.e., shewanella alginate and vibrio alginolyticus, is researched by a filter paper method and a gradient dilution method, and GC-MS analysis is further performed on several essential oils; according to comprehensive analysis, the frankincense Aman has a remarkable effect on inhibiting shewanella and vibrio alginolyticus, and reference can be provided for application of the frankincense Aman essential oil to prevention and treatment of aquatic diseases.

Description

Technical Field

[0001] This application relates to the technical field of the application of natural extracts, and particularly relates to the application of Oman frankincense essential oil in preventing and controlling aquatic diseases. Background Art

[0002] Essential oils are a general term for a class of fragrant oily secondary metabolites produced by aromatic plants during their growth and development. They are volatile and hydrolysis-resistant, and are composed of various aldehydes, phenols, alcohols, etc. compounds, mainly including terpene compounds and phenylpropene compounds, with the former being the main component. Essential oils were used as fragrances in the early days. With the in-depth research, people found that essential oils have a variety of biological activities. Currently, they are used in traditional Chinese medicine compound preparations, fragrances, insect repellents, etc., and are widely used in industries such as medicine, aquaculture, food, and cosmetics. As a substance extracted from plants, essential oils can meet the requirements of health, green, environmental protection, and pollution-free in applications.

[0003] Vibrio spp. are Gram-negative bacteria, widely distributed in inner bays, coastal areas, open ocean waters, sediments, and marine organisms, and belong to opportunistic pathogens. Marine fisheries often suffer from vibriosis caused by Vibrio infection, resulting in huge economic losses. At the same time, it will also have a negative impact on the sustainable development of the marine ecological environment. It can also infect humans through the food chain via various seafood contaminated by it, posing a serious threat to human health. Finding efficient antibiotic alternatives is very important for the aquaculture industry. Essential oils, as natural compounds with a variety of biological activities, have good application and development prospects in this regard. Summary of the Invention

[0004] The purpose of this application is to provide the application of Oman frankincense essential oil in preventing and controlling aquatic diseases to solve at least one of the above technical problems.

[0005] To achieve the above technical purpose, this application provides the application of Oman frankincense essential oil in preventing and controlling aquatic diseases.

[0006] Preferably, the application is the application of Oman frankincense essential oil in inhibiting Shewanella algae.

[0007] Preferably, the concentration of the application of Oman frankincense essential oil in inhibiting Shewanella algae is not less than 12.5 mg / mL.

[0008] Preferably, the application is the application of Oman frankincense essential oil in inhibiting Vibrio alginolyticus.

[0009] Preferably, the concentration of the Boswellia carterii Birdwood essential oil used in the application for inhibiting Vibrio alginolyticus is not less than 12.5 mg / mL.

[0010] Preferably, the Boswellia carterii Birdwood essential oil is made into a medicament.

[0011] Preferably, the medicament further comprises agriculturally acceptable excipients.

[0012] Preferably, the Boswellia carterii Birdwood essential oil comprises the following components: α-pinene, D-limonene, myrcene, sabinene, camphene, p-Mentha-1,5-dien-8-ol, β-pinene, verbenol, p-cymene, campholenal, (-)-trans-carveol, β-caryophyllene.

[0013] Preferably, the Boswellia carterii Birdwood essential oil is extracted by the following method:

[0014] S1. Weigh a certain amount of Boswellia carterii Birdwood, grind it into powder, homogenize the powdered Boswellia carterii Birdwood with pure water and sodium chloride solution, and then load it into a distillation flask.

[0015] S2. Distill at 180 °C for 2 h, let it stand for stratification, read the volume of the essential oil and collect the essential oil to obtain the Boswellia carterii Birdwood essential oil.

[0016] Compared with the prior art, the present application at least includes the following technical effects:

[0017] (1) The present application pioneered the discovery of the application of Boswellia carterii Birdwood essential oil in preventing and controlling aquatic diseases, filling the gap of this plant-derived antibiotic in this field.

[0018] (2) The present application found that Boswellia carterii Birdwood essential oil shows a good dose-effect relationship with Shewanella algae and Vibrio alginolyticus. When testing the antibacterial effect on the above two bacteria, the MIC values for the two bacteria are 12.5 mg / mL and 12.5 mg / mL respectively.

[0019] (3) Using Boswellia carterii Birdwood as the raw material, the present application further improves the application breadth of Boswellia carterii Birdwood, enhances its application value, and provides a research basis and theoretical basis for the resource utilization of Boswellia carterii Birdwood.

[0020] (4) The present application initially developed a new application of Boswellia carterii Birdwood essential oil, which has an obvious effect in inhibiting and killing aquatic pathogenic bacteria and can be used in the production of aquatic feed and natural antibacterial and bactericidal medicaments. Description of the Drawings

[0021] To more clearly illustrate the technical solutions in the embodiments of the present application or the prior art, the following will briefly introduce the drawings required for the description of the embodiments or the prior art. Obviously, the drawings in the following description are only some embodiments of the present application. For those of ordinary skill in the art, without creative efforts, other drawings can also be obtained based on these drawings.

[0022] Figure 1 It is a test result diagram of the inhibitory effect of different essential oils on Shewanella algae.

[0023] Among them, A: Frankincense essential oil; B: Ampicillin; C: DMSO; D: Neroli essential oil; E: Atractylodes lancea essential oil; F: Pogostemon cablin essential oil; G: On the left is star anise essential oil, and on the right is rosemary essential oil; H: On the left is Roman chamomile essential oil, and on the right is sage essential oil.

[0024] Figure 2 It is a test result diagram of the inhibitory effect of different essential oils on Vibrio alginolyticus.

[0025] Among them, A: Frankincense essential oil; B: Ampicillin; C: DMSO; D: Neroli essential oil; E: Atractylodes lancea essential oil; F: Pogostemon cablin essential oil; G: On the left is star anise essential oil, and on the right is rosemary essential oil; H: On the left is Roman chamomile essential oil, and on the right is sage essential oil.

[0026] Figure 3 It is an MBC diagram of Frankincense essential oil against Shewanella algae.

[0027] Figure 4 It is an MBC diagram of Frankincense essential oil against Vibrio alginolyticus.

[0028] Figure 5 It is the total ion current diagram of the volatile components of Frankincense essential oil in the present application.

[0029] Figure 6 It is a test result diagram of the inhibitory effect of different essential oils on Vibrio alginolyticus.

[0030] Among them, A: On the left is tea tree essential oil, and on the right is eucalyptus essential oil; B: On the left is lemon essential oil, and on the right is galangal essential oil; C: On the left is citronella essential oil, and on the right is 701 lavender essential oil. Detailed implementation manners

[0031] To make the objectives, features, and advantages of the present invention more obvious and understandable, the following will make a detailed description of the specific implementation manners of the present invention in combination with the embodiments. The following gives several embodiments of the present invention. However, the present invention can be implemented in many different forms and is not limited to the embodiments described herein. On the contrary, the purpose of providing these embodiments is to make the disclosure of the present invention more thorough and comprehensive:

[0032] The present application provides an application of Boswellia carterii Birdwood essential oil in preventing and controlling aquatic diseases.

[0033] Specifically, in one embodiment, the above application is the application of Boswellia carterii Birdwood essential oil in inhibiting Shewanella algae.

[0034] Furthermore, the application concentration of Boswellia carterii Birdwood essential oil in inhibiting Shewanella algae is not less than 12.5 mg / mL.

[0035] Specifically, in one embodiment, the above application is the application of Boswellia carterii Birdwood essential oil in inhibiting Vibrio alginolyticus.

[0036] Furthermore, the application concentration of Boswellia carterii Birdwood essential oil in inhibiting Vibrio alginolyticus is not less than 12.5 mg / mL.

[0037] Specifically, Boswellia carterii Birdwood essential oil can be made into a medicament.

[0038] Furthermore, in one embodiment, a Boswellia carterii Birdwood essential oil emulsion can be prepared; furthermore, the Boswellia carterii Birdwood essential oil can be mixed with a food-grade surfactant (such as Tween-80) to form a water-soluble emulsion, which is sprayed all over the pond; it can be understood that this method can improve the dispersibility of the essential oil and enhance the water body contact effect.

[0039] Furthermore, in one embodiment, a Boswellia carterii Birdwood essential oil powder (carrier adsorption type) can be prepared; furthermore, the Boswellia carterii Birdwood essential oil can be adsorbed on a porous carrier (such as zeolite powder, diatomaceous earth) and then dried and pulverized, and the powder is directly spread on the water body or mixed into the bottom mud; it can be understood that this method is convenient for storage and transportation, and the slow-release effect prolongs the action time.

[0040] Furthermore, in one embodiment, a Boswellia carterii Birdwood essential oil microcapsule preparation can be prepared; furthermore, the essential oil is encapsulated with β-cyclodextrin or gelatin to form microcapsules, and the microcapsules are put into the aquaculture water body for slow release; it can be understood that this method is beneficial to improving the stability of the essential oil and reducing the volatilization loss.

[0041] Furthermore, in one embodiment, a Boswellia carterii Birdwood essential oil feed additive can be prepared; furthermore, the Boswellia carterii Birdwood essential oil can be mixed with a carrier (such as soybean meal, starch) to form granules or premixes, and then mixed into the feed for feeding; it can be understood that this method is an internal administration method, and the internal administration absorption directly acts on the pathogen, reducing the drug residue in the water body.

[0042] Furthermore, in one of the embodiments, a compound traditional Chinese medicine mixture of Boswellia carterii can be prepared, and it can be compounded with antibacterial traditional Chinese medicine extracts such as rhubarb and scutellaria baicalensis into an aqueous solution or powder for whole-pond spraying or medicated bath; it can be understood that this method can reduce the drug resistance of single drugs and expand the antibacterial spectrum.

[0043] Furthermore, in one of the embodiments, a sustained-release gel block of Boswellia carterii essential oil can be prepared; furthermore, using sodium alginate or chitosan as the matrix, the essential oil is solidified into a gel block and suspended in a net cage or pond for slow dissolution to long-term control diseases; it can be understood that this method can reduce the frequency of drug use and is suitable for high-density aquaculture environments.

[0044] Furthermore, in one of the embodiments, in a closed-loop water system, Boswellia carterii essential oil can be used in an aerosol for fish body surface infections.

[0045] Furthermore, in one of the embodiments, probiotics can be combined to regulate water quality to form an ecological prevention and control system.

[0046] Specifically, the Boswellia carterii essential oil in this application includes the following components: α-pinene, D-limonene, myrcene, sabinene, camphene, p-Mentha-1,5-dien-8-ol, β-pinene, verbenol, 4-isopropyltoluene, campholenaldehyde, (-)-trans-pinocarveol, β-caryophyllene.

[0047] The following are some specific examples. If % is mentioned, it means by weight percentage. It should be noted that the following examples do not exhaust all possible situations, and the materials used in the following examples can be obtained from commercial channels without special instructions.

[0048] Material description:

[0049] Boswellia carterii was purchased from Shayuan South Road, Kengtou Industrial Zone, Panyu District, Guangzhou City, and is now stored in the Natural Products Laboratory. The voucher specimens are stored in the Natural Product Chemistry Laboratory of the School of Life Sciences, Huizhou University; rosemary, star anise essential oil, sage essential oil, roman chamomile essential oil, neroli essential oil, atractylodes lancea essential oil, pogostemon cablin essential oil are all purchased from Jiangxi Huashuo Essential Oil Co., Ltd.

[0050] Bacteria strains: Shewanella algae was isolated from Bahaba taipingensis; Vibrio alginolyticus was isolated from marine oysters. Both are marine vibrios and are provided by the Marine Research Laboratory of Huizhou University.

[0051] Antibiotics: Ampicillin was used as the positive control

[0052] Dimethyl sulfoxide (DMSO), LB broth, LB agar, agar, tryptone are provided by the Natural Products Laboratory of Huizhou University.

[0053] Instructions for using the instruments:

[0054] The gas chromatography-mass spectrometry (GC-MS) instrument is 7890B-5977A (Agilent, USA), the Shimadzu UV-2550 ultraviolet-visible spectrophotometer, the HP-5MS chromatographic column (30 m × 250 μm × 0.25 μm), and the Synergy H1 multimode microplate reader (Biotek, USA).

[0055] Example 1

[0056] Preparation of bacterial suspensions: The strains of S. algae and V. alginolyticus were taken out from the -80 °C refrigerator and inoculated into the prepared LB broth medium respectively, and cultured in a shaker at 35 °C for 8 h. After 8 h, the two bacterial solutions were taken out and their OD values were measured at a wavelength of 600 nm by a spectrophotometer. The bacterial solutions were diluted with normal saline to an OD value of 0.05 or less and used immediately after preparation.

[0057] Screening of essential oils with antibacterial activity by the filter paper method: 1 mL of the bacterial solution was evenly spread on the LB agar plate and left to dry; sterile filter papers with a diameter of 6 mm were placed equidistantly on a sterile petri dish; 5 μL of the essential oil was respectively pipetted and dropped on the filter papers, and the filter papers were clamped with sterilized forceps and placed on the corresponding LB agar plates containing bacteria; 5 μL of ampicillin was used as a positive control. The plates were inverted and cultured in an incubator at 35 °C, and the results were checked every 8 h and observed three times until the culture reached 24 h. Three groups of experiments were repeated in parallel, and the average value was taken to evaluate the antibacterial effect of the plant essential oil.

[0058] Measurement of the inhibition zone: The diameter of the inhibition zone was measured by the cross method to determine the average value, with the unit of mm. Two parallels were set. The larger the diameter of the inhibition zone, the better the inhibitory effect of the essential oil on the strain; on the contrary, the smaller the diameter of the inhibition zone, the worse the inhibitory effect. In the inhibition zone test, filter papers with a diameter of 6 mm were used, and the sensitivity of bacteria to the essential oil was judged according to the size of the inhibition zone. The result judgment was based on the "Performance Standards for Antimicrobial Susceptibility Testing" promulgated by the National Committee for Clinical Laboratory Standards (NCCLS) of the United States: a diameter > 20 mm was extremely sensitive, 15 - 20 mm was highly sensitive, 10 - 15 mm was moderately sensitive, and < 10 mm was lowly sensitive.

[0059] Determination of minimum inhibitory concentration (MIC): The detection was carried out by the microdilution method. The bacterial solution was diluted with LB broth to an OD value of 0.05 or less. Then, the bacterial solution and the diluted essential oil (the final concentrations of the essential oil were: 100, 50, 25, 12.5, 6.25, 3.13, 1.56, 0.78, 0.39, 0.20 mg / mL) were added to a 96-well plate and incubated in an incubator at 35 °C for 24 h. It was taken out every 8 h for observation. If the liquid in the well was clear to the naked eye, it was the MIC.

[0060] Determination of minimum bactericidal concentration (MBC): According to the inhibition rate, 2 μL of samples with different concentrations were taken and added to the LB agar plate in the drawn area, with three parallels set; it was inverted and incubated in an incubator at 35 °C for 8 - 24 h, and the colony situation in the plate was observed every 8 h.

[0061] Example 2

[0062] Gas chromatography - mass spectrometry

[0063] Chromatographic conditions (GC): The inlet temperature was 250 °C, the carrier gas was He gas, the flow rate was 1 mL / min, and there was no split; Temperature programming: The initial column temperature was 40 °C, held for 3 min, then increased to 160 °C at a rate of 5 °C / min, and then increased to 200 °C at a rate of 10 °C / min and held for 10 min.

[0064] Mass spectrometry conditions (MS): The ion source temperature was 230 °C, the electron impact ion source, the electron impact energy was 70 eV, the transfer interface temperature was 250 °C, the MS quadrupole temperature was 150 °C, and the scanning range was 50 - 500 m / z.

[0065] The extracted Boswellia carterii Birdw essential oil was diluted 10 times, and 0.2 μL of the essential oil dilution was manually injected, with 3 parallels set. The results were expressed as mean ± standard deviation.

[0066] Example 3

[0067] Qualitative analysis of volatile components of essential oil

[0068] MS identification: The mass spectra of each component of Boswellia carterii Birdw essential oil were automatically retrieved from the NIST standard mass spectrometry library and identified by combining manual parsing, and their retention times, retention indices, molecular formulas, etc. were compared.

[0069] Retention index (RI) identification: Under the same gas chromatography - mass spectrometry conditions, n - alkanes and Boswellia carterii Birdw essential oil were analyzed simultaneously, and the retention times of n - alkanes (C8 - C 20 ) and each component of Boswellia carterii Birdw essential oil were measured. Through the retention times of n - alkanes, the retention indices of each component of the essential oil were calculated. The calculation formula of the retention index is as follows:

[0070] RI = 100Z + ((RT X - RT Z ) × 100) / (RT (Z+1) - RT Z )(Formula 1)

[0071] Z: The number of all carbon atoms of the n - alkane adjacent to the unknown component peak before it

[0072] RT x : Retention time of the unknown component

[0073] RT z : Retention time of the n - alkane adjacent to the unknown component peak before it

[0074] Z + 1: The number of all carbon atoms of the n - alkane adjacent to the unknown component peak after it

[0075] RT Z+1 : Retention time of the n - alkane adjacent to the unknown component peak after it

[0076] Search in the NIST mass spectrometry database according to the RI and CAS number of each component, and confirm with the compounds that match the retention index in the literature to identify the volatile components of the unknown Oman frankincense essential oil.

[0077] Example 4

[0078] Semi - quantitative analysis of the volatile components of the essential oil

[0079] The volatile components of the plant essential oil are detected and analyzed by GC - MS, and the total ion chromatogram of its volatile components can be obtained. Search through the NIST / WILEY spectral library and use the area normalization method to semi - quantitatively analyze the relative content of each component, and identify the volatile substances from it.

[0080] The relative content of each separated component = the peak area of each separated component / the total peak area × 100% (Formula 2)

[0081] Result analysis

[0082] I. Result analysis of antibacterial activity

[0083] (1) Filter paper method

[0084] The antibacterial effects of essential oils such as Oman frankincense on Shewanella algae are as Figure 1As shown, the sensitivity levels and inhibition zone diameters are as follows: Boswellia carterii Birdwood essential oil (22.25 mm, extremely sensitive) > Pogostemon cablin (Blanco) Benth. essential oil (17 mm, highly sensitive) > Atractylodes lancea (Thunb.) DC. essential oil (16 mm, highly sensitive) > Anthemis nobilis L. essential oil (13.67 mm, moderately sensitive) > Citrus aurantium L. var. amara Engl. essential oil (13.25 mm, moderately sensitive) > Ampicillin (12 mm, moderately sensitive) > Salvia officinalis L. essential oil (10.5 mm, moderately sensitive) > Rosmarinus officinalis L. essential oil (8.17 mm, slightly sensitive) > Illicium verum Hook.f. essential oil (6.33 mm, slightly sensitive).

[0085] The antibacterial effects of essential oils such as Boswellia carterii Birdwood on Vibrio alginolyticus are as follows Figure 2 As shown, the sensitivity levels and inhibition zone diameters are as follows: Boswellia carterii Birdwood essential oil (26.17 mm, extremely sensitive) > Anthemis nobilis L. essential oil (12.00 mm, moderately sensitive) > Salvia officinalis L. essential oil (11.83 mm, moderately sensitive) > Atractylodes lancea (Thunb.) DC. essential oil (10.34 mm, moderately sensitive) > Rosmarinus officinalis L. essential oil (9.17 mm, slightly sensitive) > Ampicillin (9 mm, slightly sensitive) > Pogostemon cablin (Blanco) Benth. essential oil (8.34 mm, slightly sensitive > Illicium verum Hook.f. essential oil (6 mm, slightly sensitive) > Citrus aurantium L. var. amara Engl. essential oil (6 mm, slightly sensitive).

[0086] Table 1 Anti-vibrio activities of different essential oils (unit / mm)

[0087]

[0088] Further referring to Table 1, from the data in Table 1, it can be seen that Boswellia carterii Birdwood has the best antibacterial effect, reaching extremely sensitive (inhibition zone > 20 mm) for both bacteria. Among them, its antibacterial effect on Vibrio alginolyticus is the best, with an inhibition zone of 26.17 mm. The antibacterial effect of Shewanella algae is the second best, at 22.25 mm.

[0089] (2) Determination of minimum inhibitory concentration (microdilution method)

[0090] Upon observation, the MIC of Boswellia carterii Birdwood essential oil against S. algae and V. alginolyticus is shown in Table 2, both being 12.5 mg / mL. The MBC of Boswellia carterii Birdwood essential oil against S. algae and V. alginolyticus is shown in Table 2 and Figure 3 as follows, both being 50 mg / mL.

[0091] Table 2 MIC / MBC values of Boswellia carterii Birdwood essential oil against two vibrios (unit: mg / mL)

[0092]

[0093] II. Analysis of volatile components of essential oils

[0094] The volatile components of frankincense essential oil from Oman were analyzed under the above GC-MS conditions, and the total ion current chromatogram of frankincense essential oil from Oman was obtained (see Figure 4 ). Through NIST / WILEY spectral library retrieval and using the area normalization method, 54 volatile compounds were identified from frankincense essential oil from Oman, including terpenes (30), alcohols (12), esters (3), ketones (3), aromatics (3), ethers (2), and aldehydes (1), as shown in Figure 5 . As can be seen from Table 3, the components with relative contents higher than 1% are α-pinene (65.53%) and D-limonene (5.00%), followed by myrcene (2.51%), sabinene (2.42%), camphene (2.34%), p-Mentha-1,5-dien-8-ol (2.30%), β-pinene (1.79%), verbenol (1.40%), etc., accounting for 82.29% of the total content of the compounds, which are the main components of frankincense essential oil from Oman.

[0095] Table 3 Components of Frankincense Essential Oil from Oman

[0096]

[0097]

[0098]

[0099] Table 4 Components of Citronella Essential Oil

[0100]

[0101]

[0102] Table 5 Components of Alpinia officinarum Hance Essential Oil

[0103]

[0104]

[0105] Table 6 Components of Eucalyptus Essential Oil

[0106]

[0107] As can be seen from Table 3 above, the main component in frankincense essential oil from Oman is α-pinene with the highest proportion. There are relevant literatures disclosing that this component can damage the bacterial cell membrane structure and interfere with the bacterial metabolic process. In order to verify whether the content of α-pinene is positively correlated with the inhibitory effect of frankincense essential oil from Oman on Shewanella algae and Vibrio alginolyticus, this application further supplemented a comparative experiment, which can be seen in Figure 6 and Tables 4-6; among them Figure 6The bacteriostatic effect diagrams of tea tree essential oil, eucalyptus essential oil, lemon essential oil, galangal essential oil, citronella essential oil, and 701 lavender essential oil on Vibrio alginolyticus are as follows: lemon essential oil is highly sensitive (16.67 mm) > citronella essential oil is moderately sensitive (12.50 mm) > 701 lavender essential oil is moderately sensitive (12.33 mm) > eucalyptus essential oil is moderately sensitive (11.33 mm) > Australian tea tree essential oil is moderately sensitive (10.50 mm) > galangal essential oil is slightly sensitive (8.00 mm). Further, in this application, GC-MS analysis was carried out on citronella essential oil, eucalyptus essential oil, and galangal essential oil. Among them, α-pinene was not detected in citronella essential oil, the content of α-pinene detected in eucalyptus essential oil was 4.07%, and the content of α-pinene detected in galangal essential oil was 6.83%. As can be seen from the above, the α-pinene content in galangal essential oil is significantly higher than that in citronella essential oil and eucalyptus essential oil. In this regard, the inventor believes that the bacteriostatic effect of a specific essential oil on a specific strain does not depend on the content of a single component, but is closely related to the component combination of the essential oil, the extraction process, and the characteristics of the target strain. This application pioneered the discovery of the application of Boswellia carterii Birdw essential oil in preventing and controlling aquatic diseases, and further discovered the application of Boswellia carterii Birdw essential oil in inhibiting two aquatic pathogenic bacteria, Shewanella algae and Vibrio alginolyticus, filling the blank of this plant-derived antibiotic, Boswellia carterii Birdw essential oil, in this field. Furthermore, from the above test results, it can be seen that Boswellia carterii Birdw essential oil shows extremely significant advantages in inhibiting these two aquatic pathogenic bacteria, Shewanella algae and Vibrio alginolyticus.

[0108] It should be noted that, without conflict, the embodiments in this application and the features in the embodiments can be combined with each other.

[0109] The above are only preferred embodiments of this application, and do not impose any form of limitation on this application. Any simple modification, equivalent change, and modification made to the above embodiments based on the technical essence of this application all belong to the scope of the technical solution of this application.

Claims

1. Application of Omani frankincense essential oil in preventing and treating aquatic diseases.

2. The application of Omani frankincense essential oil in preventing and treating aquatic diseases according to claim 1, characterized in that: The application is the application of Omani frankincense essential oil in inhibiting Shewanella algae.

3. The application of Omani frankincense essential oil in preventing and treating aquatic diseases according to claim 2, characterized in that: The Omani frankincense essential oil is used in an application concentration of not less than 12.5 mg / mL for inhibiting Shewanella algae.

4. The application of Omani frankincense essential oil in preventing and treating aquatic diseases according to claim 1, characterized in that: The application is the application of Omani frankincense essential oil in inhibiting Vibrio alginolyticus (V. alginolyticus).

5. The application of Omani frankincense essential oil in preventing and treating aquatic diseases according to claim 4, characterized in that: The Omani frankincense essential oil is used in an application concentration of not less than 12.5 mg / mL for inhibiting Vibrio alginolyticus (V. alginolyticus).

6. The use of Omani frankincense essential oil in preventing and treating aquatic diseases according to any one of claims 1 to 5, characterized in that: The Omani frankincense essential oil is prepared into a medicament.

7. The use of Omani frankincense essential oil in preventing and treating aquatic diseases according to claim 6, characterized in that: The medicament further comprises an agriculturally acceptable adjuvant.

8. The use of Omani frankincense essential oil in preventing and treating aquatic diseases according to any one of claims 1 to 5, characterized in that: The Omani frankincense essential oil comprises the following components: alpha-pinene, D-limonene, myrcene, sabinene, camphene, p-Mentha-1,5-dien-8-ol, beta-pinene, verbenol, 4-isopropyltoluene, camphorenal, (-)-trans-pine carveol and beta-caryophyllene.

9. The use of Omani frankincense essential oil in preventing and treating aquatic diseases according to any one of claims 1 to 5, characterized in that: The Omani frankincense essential oil is extracted by the following method: S1. Weigh a certain amount of Omani frankincense, grind the components, homogenize the powdered Omani frankincense with pure water and sodium chloride solution, and then put it into a distillation bottle; S2. Distilling at 180° C. for 2 h, reading the volume of the essential oil after standing and stratifying, and collecting the essential oil to obtain the Omani frankincense essential oil.

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