Microbial film capable of promoting plant growth and preparation method thereof

Abstract

The invention discloses a microbial film capable of promoting plant growth and a preparation method thereof, and particularly relates to the field of soil fertilization. The microbial film comprises the following raw materials in percentage by mass: 44-48% of glutinous rice flour, 6-15% of seaweed meal, 5-15% of a polysaccharide material, 12-17% of natural cellulose, 3-4% of nanoparticles and 13-18% of probiotics. The preparation method comprises the following steps: adding the glutinous rice flour and the polysaccharide material into clear water; adding the seaweed meal, the natural cellulose and the nano-particles, and continuously stirring; finally, adding the probiotics, and lightly and uniformly stirring; pouring the mixed solution into a clean mold for quick freezing; putting the membrane material into a freeze dryer for drying; and checking the dried membrane material to ensure that the thickness, the uniformity and the microorganism survival rate of the membrane material meet the requirements. According to the technical scheme, the problem that a single microbial agent is short in validity period is solved, the air permeability of plant roots can be improved, meanwhile, water is reasonably released, and the survival rate of plants can be effectively increased.

Description

Technical Field

[0001] The present invention relates to the field of soil fertilization, and particularly to a microbial film with the function of promoting plant growth and its preparation method. Background Art

[0002] Barren soils usually lack sufficient nutrients, microbial activity, and good physical structure, resulting in restricted plant growth. Factors such as long-term agricultural activities, soil erosion, drought, and over-tillage have made a large amount of land barren and even unable to sustain normal plant growth. This situation urgently requires effective soil improvement technologies to restore soil fertility and ecological functions.

[0003] Microorganisms play a crucial role in the soil ecosystem. Probiotics can not only promote nutrient cycling, such as nitrogen fixation, phosphorus solubilization, and potassium solubilization, but also inhibit pathogenic microorganisms and enhance the stress resistance of plants. In addition, microorganisms also play an important role in decomposing organic matter, improving soil structure, and enhancing water retention capacity. However, the use of a single microbial agent often has the problem of short activity and validity period. Summary of the Invention

[0004] The present invention aims to provide a microbial film with the function of promoting plant growth and its preparation method, which solves the problem of short validity period of a single microbial agent.

[0005] To achieve the above object, a technical solution provided by the present invention is as follows: A microbial film with the function of promoting plant growth, comprising raw materials in the following mass percentages: 44 - 48% glutinous rice flour, 6 - 15% seaweed powder, 5 - 15% polysaccharide materials, 12 - 17% natural cellulose, 3 - 4% nanoparticles, and 13 - 18% probiotics.

[0006] Principle and Effect of the Technical Solution: Glutinous rice flour, as a natural binder, has good adhesiveness and film-forming property. Glutinous rice flour not only provides structural support for other components but also forms a flexible film layer, ensuring the stability of the film. It provides the basic framework of the film, making the film have a certain toughness and ductility. At the same time, it enhances the biodegradability of the film, enabling it to gradually decompose in the soil and avoid long-term residue.

[0007] Seaweed powder is rich in polysaccharides, amino acids, minerals, and vitamins, and has good water absorption and moisture retention properties. Seaweed powder can provide additional nutrients for microorganisms and promote plant growth. At the same time, it improves the water retention capacity of the film, prolongs the active time of microorganisms, and has a slow-release effect. And it provides trace elements required by plants and improves the soil microenvironment.

[0008] Polysaccharide materials are natural thickeners with good film-forming properties and protective functions. They can effectively enhance the physical properties of the film; and increase the stability and strength of the film, prevent the film from rupturing prematurely, and ensure the effective release of probiotics during use. At the same time, they provide a protective layer for microorganisms, enhance the survival rate of microorganisms in the film, and reduce the impact of environmental stress on their activity.

[0009] Natural cellulose has good water absorption and air permeability. Cellulose can not only improve the physical properties of the film, but also provide attachment points for microorganisms; and improve the air permeability of the film, promote oxygen exchange in the soil, and provide a better living environment for microorganisms. At the same time, by decomposing into organic matter, it improves the soil structure and promotes soil water retention and nutrient supply.

[0010] Nanoparticles have strong adsorption ability and antibacterial effects. Nanoparticles can stabilize the structure of the film, and at the same time have antioxidant and antibacterial properties, which helps to enhance the activity of microorganisms. Stabilizing the structure of the film can delay the release rate of microorganisms and moisture, improve the long-term effect of the film; and also provide a certain antibacterial effect to protect probiotics from harmful pathogenic microorganisms.

[0011] Furthermore, the polysaccharide material is a mixture of xanthan gum and citrus protopectin.

[0012] Furthermore, the natural cellulose is one or more of lignocellulose, seed fiber, and phloem fiber.

[0013] Furthermore, the nanoparticles are a mixture of silica and zinc oxide.

[0014] Furthermore, the probiotics are a mixture of nitrogen-fixing bacteria, phosphate-solubilizing bacteria, potassium-solubilizing bacteria, Bacillus, and yeast.

[0015] Through the above settings, nitrogen-fixing bacteria can promote nitrogen fixation, provide the nitrogen source required by plants, and improve soil fertility. Phosphate-solubilizing bacteria can dissolve insoluble phosphates in the soil and improve the utilization rate of phosphorus. Potassium-solubilizing bacteria can release potassium elements and promote the normal growth and development of plants. Bacillus has a strong biological control effect, inhibits pathogenic microorganisms, and promotes soil health. Yeast promotes plant growth and improves stress resistance by secreting plant hormones and antioxidant substances.

[0016] Through the synergistic effect of these materials in this scheme, the microbial film can activate the microbial activity in barren soil, improve the soil structure, enhance the soil fertility, and promote the growth and stress resistance of plants, and finally achieve the purpose of restoring the soil ecosystem and increasing agricultural production.

[0017] Another technical solution provided by the present invention is as follows: A preparation method of a microbial film with the function of promoting plant growth, comprising the following steps: S1. Dissolution and mixing: Add 44 - 48% of glutinous rice flour and 5 - 15% of polysaccharide materials into an appropriate amount of clear water, and stir evenly until a viscous solution is formed; gradually add 6 - 15% of seaweed powder, 12 - 17% of natural cellulose, and 3 - 4% of nanoparticles, and continue to stir to ensure that the solid materials are evenly dispersed; finally, add 13 - 18% of probiotics and gently stir evenly to ensure that the probiotics are evenly distributed in the solution; S2. Film formation: Pour the mixture in step S1 into a clean mold or tray, ensure that the liquid is flat and bubble-free; place the mold in a low-temperature environment for rapid freezing to ensure that the solution forms a uniform solid film; S3. Freeze-drying: Put the frozen film material into a freeze-dryer for drying; S4. Trimming and packaging: Check the dried film material to ensure that its thickness, uniformity, and microbial survival rate meet the requirements; cut the freeze-dried microbial slow-release film into the required shape and size; finally, package the finished product and store it in a dry and low-temperature environment to ensure the activity of microorganisms and the performance of the film during transportation and storage.

[0018] Principle and effect of the technical solution: The freeze-drying method adopted in the present invention can effectively remove moisture while maximizing the retention of the activity of microorganisms. During the freeze-drying process, water is directly changed from a solid state to a gaseous state through sublimation, avoiding the presence of liquid water. This not only helps to protect microbial cells from heat damage but also maintains the structure and function of the film. In addition, the drying process under a vacuum environment can prevent adverse reactions such as oxidation, further enhancing the stability and shelf life of the microbial film. The microbial film prepared by the freeze-drying method can effectively improve the survival rate of microorganisms and uniformly release microorganisms under different humidity conditions. The film has excellent air permeability, can meet the oxygen demand of plant roots, and at the same time provide sufficient water and nutrient support, thus significantly improving the growth and stress resistance of plants. The freeze-drying method can maximize the retention of the activity of microorganisms and ensure that it can still play a good ecological restoration role in extreme environments.

[0019] Furthermore, the freezing temperature in step S2 is set to -20°C to -40°C.

[0020] Furthermore, the method of step S3 is as follows: Heating and pressure-boosting stage: Place the frozen film material in a vacuum environment, gradually increase the temperature to sublime the ice crystals and remove moisture; the temperature in this stage is set between -40°C and -20°C; Drying stage: Further remove the residual moisture in the vacuum environment to ensure that the film material is completely dry and the microorganisms remain active; the drying time is 24 - 48 hours.

[0021] With the above settings, residual moisture is further removed in a vacuum environment. The role of this stage is to ensure that the membrane material is completely dry and the microorganisms remain active. By controlling the drying time, the relationship between moisture removal and microorganism protection can be balanced. If the drying time is too short, the membrane material may not be thoroughly dehydrated, affecting the quality of the microbial membrane; if it is too long, it may lead to the death or reduced activity of the microorganisms. Therefore, a drying time of 24 - 48 hours can ensure good drying effects while protecting the activity of the microorganisms.

[0022] Compared with the prior art, the beneficial effects of this solution are as follows: 1. This solution provides a microbial membrane that promotes plant growth. By embedding microorganisms, it promotes the growth of plant roots, especially activates the activity of microorganisms in harsh environments, improves soil structure, enhances soil fertility, and promotes plant growth and stress resistance, ultimately achieving the goal of restoring soil ecology and increasing agricultural yields. The microbial membrane can provide the nutrients and protection required by plants, while enhancing the water absorption capacity and stress resistance of their roots.

[0023] 2. By encapsulating and slowly releasing probiotics, the microbial membrane can effectively improve the survival rate and function of microorganisms in infertile soils, release beneficial microorganisms and nutrients for a long time, activate the microbial community in the soil, and restore the soil ecological balance. In this way, the microbial membrane can improve soil structure, promote the decomposition of organic matter, increase the nutrient content of the soil, and promote the growth of plant roots by improving the soil microbial environment, thus effectively promoting plant growth and increasing soil productivity. This technology not only helps to restore the biological activity of infertile soils but also can improve the yield and quality of crops, with broad application prospects. Specific implementation manners

[0024] The present invention will be further described in detail below through specific implementation manners: Example 1 A microbial membrane that promotes plant growth comprises raw materials in the following mass percentages: 45% rice flour, 8% seaweed powder, 10% polysaccharide materials, 17% natural cellulose, 4% nanoparticles, and 16% probiotics. Among them, the polysaccharide material is citrus protopectin. The natural cellulose is a mixture of wood cellulose, seed fiber, and bast fiber, and the mass percentages of wood cellulose, seed fiber, and bast fiber are 52%, 8%, and 40% respectively. The nanoparticles are a mixture of silicon dioxide and zinc oxide, and the mass percentage of silicon dioxide is 75% and that of zinc oxide is 25%. The probiotics are a mixture of nitrogen-fixing bacteria, phosphorus-solubilizing bacteria, potassium-solubilizing bacteria, Bacillus, and yeast, and the mass percentages of nitrogen-fixing bacteria, phosphorus-solubilizing bacteria, potassium-solubilizing bacteria, Bacillus, and yeast are 25%, 25%, 20%, 15%, and 15% respectively.

[0025] Example 2 A microbial film with the function of promoting plant growth, comprising raw materials in the following mass percentages: 48% rice flour, 10% seaweed powder, 8% polysaccharide materials, 17% natural cellulose, 3% nanoparticles, and 14% probiotics. Among them, the polysaccharide materials are a mixture of xanthan gum and citrus protopectin, and the mass percentages of xanthan gum and citrus protopectin are 75% and 25% respectively. The natural cellulose is a mixture of lignocellulose, seed fiber, and phloem fiber, and the mass percentages of lignocellulose, seed fiber, and phloem fiber are 52%, 8%, and 40% respectively. The nanoparticles are a mixture of silica and zinc oxide, and the mass percentages of silica and zinc oxide are 75% and 25% respectively. The probiotics are a mixture of nitrogen-fixing bacteria, phosphorus-solubilizing bacteria, potassium-solubilizing bacteria, bacillus, and yeast, and the mass percentages of nitrogen-fixing bacteria, phosphorus-solubilizing bacteria, potassium-solubilizing bacteria, bacillus, and yeast are 25%, 25%, 20%, 15%, and 15% respectively.

[0026] Example 3 A microbial film with the function of promoting plant growth, comprising raw materials in the following mass percentages: 45% rice flour, 6% seaweed powder, 15% polysaccharide materials, 12% natural cellulose, 4% nanoparticles, and 18% probiotics. Among them, the polysaccharide materials are a mixture of xanthan gum and citrus protopectin, and the mass percentages of xanthan gum and citrus protopectin are 75% and 25% respectively. The natural cellulose is a mixture of lignocellulose and phloem fiber, and the mass percentages of lignocellulose and phloem fiber are 65% and 35% respectively. The nanoparticles are a mixture of silica and zinc oxide, and the mass percentages of silica and zinc oxide are 75% and 25% respectively. The probiotics are a mixture of nitrogen-fixing bacteria, phosphorus-solubilizing bacteria, potassium-solubilizing bacteria, bacillus, and yeast, and the mass percentages of nitrogen-fixing bacteria, phosphorus-solubilizing bacteria, potassium-solubilizing bacteria, bacillus, and yeast are 25%, 25%, 20%, 15%, and 15% respectively.

[0027] Example 4 A microbial film with the function of promoting plant growth, comprising raw materials in the following mass percentages: 44% rice flour, 12% seaweed powder, 10% polysaccharide materials, 17% natural cellulose, 4% nanoparticles, 13% probiotics. Among them, the polysaccharide materials are a mixture of xanthan gum and citrus protopectin, and the mass percentages of xanthan gum and citrus protopectin are 75% and 25% respectively. The natural cellulose is a mixture of lignocellulose and phloem fiber, and the mass percentages of lignocellulose and phloem fiber are 65% and 35% respectively. The nanoparticles are a mixture of silica and zinc oxide, and the mass percentages of silica and zinc oxide are 75% and 25% respectively. The probiotics are a mixture of nitrogen-fixing bacteria, phosphorus-solubilizing bacteria, potassium-solubilizing bacteria, Bacillus, and yeast, and the mass percentages of nitrogen-fixing bacteria, phosphorus-solubilizing bacteria, potassium-solubilizing bacteria, Bacillus, and yeast are 25%, 25%, 20%, 15%, and 15% respectively.

[0028] Example 5 A microbial film with the function of promoting plant growth, comprising raw materials in the following mass percentages: 48% rice flour, 15% seaweed powder, 5% polysaccharide materials, 12% natural cellulose, 3% nanoparticles, 17% probiotics. Among them, the polysaccharide materials are a mixture of xanthan gum and citrus protopectin, and the mass percentages of xanthan gum and citrus protopectin are 75% and 25% respectively. The natural cellulose is a mixture of lignocellulose and phloem fiber, and the mass percentages of lignocellulose and phloem fiber are 65% and 35% respectively. The nanoparticles are a mixture of silica and zinc oxide, and the mass percentages of silica and zinc oxide are 75% and 25% respectively. The probiotics are a mixture of nitrogen-fixing bacteria, phosphorus-solubilizing bacteria, potassium-solubilizing bacteria, Bacillus, and yeast, and the mass percentages of nitrogen-fixing bacteria, phosphorus-solubilizing bacteria, potassium-solubilizing bacteria, Bacillus, and yeast are 25%, 25%, 20%, 15%, and 15% respectively.

[0029] Control group A microbial film with the function of promoting plant growth, comprising raw materials in the following mass percentages: 45% rice flour, 7% seaweed powder, 12% polysaccharide materials, 15% natural cellulose, 4% nanoparticles, 17% probiotics. The polysaccharide materials are a mixture of xanthan gum and citrus protopectin, and the mass percentages of xanthan gum and citrus protopectin are 75% and 25% respectively. The natural cellulose is a mixture of lignocellulose and phloem fiber, and the mass percentages of lignocellulose and phloem fiber are 65% and 35% respectively. The nanoparticles are a mixture of silica and zinc oxide, and the mass percentages of silica and zinc oxide are 68% and 32% respectively. The probiotics are a mixture of nitrogen-fixing bacteria, phosphorus-solubilizing bacteria, potassium-solubilizing bacteria, Bacillus, and yeast, and the mass percentages of nitrogen-fixing bacteria, phosphorus-solubilizing bacteria, potassium-solubilizing bacteria, Bacillus, and yeast are 25%, 25%, 20%, 15%, and 15% respectively.

[0030] The microbial slow-release membranes prepared in Examples 1-5 and the comparative examples were compared under the same experimental conditions, and the results in Tables 1 and 2 below were obtained. The main aspects observed in the experiment were as follows: 1. Microbial survival rate (after 24 hours, 48 hours, and 72 hours); 2. Microbial release amount; 3. Reproduction quantity of microorganisms (under different temperature and humidity conditions).

[0031] The specific experimental methods are as follows: 1. Microbial survival rate test method: Expose the microbial slow-release membrane to conditions of 79% humidity and 30 °C temperature, regularly sample, and count the activity of microorganisms by the plate culture method.

[0032] 2. Microbial release test method: Treat the microbial slow-release membrane with environments of different humidities (such as soils with different water contents), and measure the amount of microorganisms released at different time points.

[0033] 3. Reproduction quantity of microorganisms: Compare the number of bacteria before and after the experiment.

[0034] Table 1 Table 2 It can be seen from Tables 1 and 2 that: 1. Microbial survival rate analysis: Example 1 showed the best microbial survival rate. Especially under high humidity conditions, the survival rate of microorganisms remained at a relatively high level. This performance was attributed to its excellent composite material design. Glutinous rice flour and natural cellulose provided stable structural support, ensuring the flexibility and moisture retention of the membrane, and avoiding the occurrence of cracking. The natural polysaccharide component in seaweed powder further enhanced the moisture retention effect of the membrane, while the nanoparticles enhanced the stability of the membrane, regulated the release of moisture and the slow release of microorganisms.

[0035] In Example 2, due to the addition of xanthan gum, although its survival rate was slightly lower than that of Example 1, indicating its weaker moisture resistance. In a humid environment, the survival of microorganisms was slightly affected, but it could still support the reproduction and survival of microorganisms well.

[0036] Although the microbial survival rates and reproduction quantities of Examples 3-5 and the comparative example were different, they generally showed relatively high survival rates and reproduction potentials. Especially in Example 5, due to the relatively high proportion of seaweed powder and probiotics in its formula, the microbial survival rate reached 96% (24h), showing strong moisture resistance and reproduction ability.

[0037] 2. Microbial release amount analysis: Example 1 performed best in terms of microbial release, and its stable release rate ensured that the microorganisms could be evenly released under different humidity conditions. Its microbial release was higher than that of other examples, reflecting the superiority of its material design, which could provide continuous microbial release in different environments and promote long-term improvement of soil health.

[0038] The microbial release in Example 2 is slightly lower, which is due to the high release rate of the polysaccharide material, which causes the microorganisms to be released rapidly in a short time. Nevertheless, the rapid release of microorganisms still has a certain effect on soil improvement, especially in soils with high humidity, which can accelerate the action of microorganisms.

[0039] The amount of microorganisms released in Example 3 and Example 4 is comparable to that in Example 2, but the release rate is more balanced, and is suitable for scenarios requiring stable and continuous release of microorganisms.

[0040] The microbial release amounts of Example 5 and the comparative example are relatively low, indicating that their release rates are relatively fast and suitable for soil environments that require rapid repair, but their performance in long-term release is relatively weak.

[0041] 3. Analysis of microbial reproduction quantity: The number of microorganisms propagated in Example 1 is the highest among all the examples, which fully demonstrates its comprehensive advantages in microbial survival rate and release amount. The uniform microbial release of this example not only improves the microbial activity in the soil, but also promotes the long-term propagation of microorganisms and improves the soil structure.

[0042] The reproduction numbers of Examples 2, 3, 4 and the comparative example are relatively low. Although they can release more microorganisms in the short term, due to the faster release rate, the long-term reproduction number of microorganisms is slightly lower than that of Example 1. In particular, the comparative example, although the release amount is high, the reproduction number is significantly lower than that of Example 1, indicating that its microorganisms fail to continue to reproduce after release.

[0043] In summary, Example 1, by virtue of its efficient and stable microbial release system, exhibits the best microbial survival rate, release efficiency and reproduction number, and is the most suitable formulation for long-term soil remediation and plant growth promotion.

[0044] Embodiments 2 and 3 can be effective in a relatively short time by accelerating the release of microorganisms, and are suitable for scenarios where rapid soil improvement is required. However, the long-term survival and reproduction capabilities of the microorganisms are relatively weak, and a too fast release rate may lead to the loss of soil microorganisms.

[0045] Examples 4, 5 and the comparative examples showed good adaptability under different environmental conditions. In particular, they had strong survival rates and reproductive abilities in humid environments and were suitable for the remediation needs of different soil types. Due to the higher proportion of probiotics and seaweed powder, Example 5 had better long-term stability under humid conditions.

[0046] The microbial films in the present invention play a crucial role in improving soil structure. Their activities directly affect the physical, chemical, and biological properties of the soil, thereby promoting the stability and functionality of the soil structure. The following are the main mechanisms and manifestations of the microbial films in improving soil structure: 1. Promote the decomposition of organic matter and the formation of humus Decomposition of organic matter: Microorganisms (such as bacteria, fungi, actinomycetes) decompose organic substances such as plant and animal residues and root exudates, and convert them into small molecule compounds (such as sugars, amino acids) and stable humus.

[0047] Functions of humus: As a natural cementing agent, it promotes the adhesion of soil particles (such as clay particles, silt particles) into stable aggregates (granular structure).

[0048] Enhance the water and fertilizer retention capacity of the soil and reduce soil compaction.

[0049] 2. Secrete cementing substances to enhance the stability of aggregates Microbial secretions: Bacteria and fungi secrete viscous substances such as polysaccharides, proteins, and lipids (such as gummy polysaccharides, glomalin-related proteins) during metabolism.

[0050] These substances bind soil particles like "glue" to form water-stable aggregates (granules with strong resistance to water erosion).

[0051] Fungal hyphae can also fix soil particles through physical entanglement.

[0052] 3. Improve pore structure and air permeability Pore formation: The combined action of microbial activities (such as decomposing organic matter, hyphal expansion) and root growth forms micro-pores and macro-pores in the soil.

[0053] Enhance soil air permeability, drainage capacity, and root penetration.

[0054] Reduce soil compaction and lower tillage resistance.

[0055] 4. Promote nutrient cycling and plant growth Nutrient release: Microorganisms decompose organic matter to release nutrients such as nitrogen, phosphorus, and potassium, promoting the development of plant roots.

[0056] Synergy between roots and microorganisms: Plant root exudates attract beneficial microorganisms (such as rhizobia, mycorrhizal fungi) to form symbiotic relationships.

[0057] The hyphal network of mycorrhizal fungi (such as arbuscular mycorrhizae) can extend deep into the soil, enhancing soil aggregation.

[0058] 5. Inhibiting soil degradation and erosion Erosion resistance: A stable aggregate structure can reduce wind and water erosion.

[0059] Saline-alkali soil improvement: Certain microorganisms (such as acid-producing bacteria) reduce soil pH through metabolic activities to alleviate salinization.

[0060] 6. Synergistic effects with other soil organisms Mutual benefit with earthworms: Microorganisms decompose organic matter to provide food for earthworms, and the excrement of earthworms (vermicast) further promotes microbial activities, forming a virtuous cycle.

[0061] Maintenance of biodiversity: Soils with high microbial diversity are more resistant to stress (such as drought resistance, pathogen resistance).

[0062] Soil microorganisms directly or indirectly promote the formation of soil aggregates, improve pore structure, water retention capacity, and erosion resistance by decomposing organic matter, secreting cementing substances, and forming symbiotic networks. A healthy microbial community is the core of soil sustainable productivity and the key regulatory object of ecological agriculture and soil remediation.

[0063] The above are only embodiments of the present invention. Common general knowledge such as specific structures and / or characteristics in the solution is not described in detail herein. It should be noted that for those skilled in the art, without departing from the structure of the present invention, several modifications and improvements can be made, which should also be regarded as the protection scope of the present invention, and these will not affect the implementation effect of the present invention and the practicability of the patent. The protection scope required by this application should be based on the content of its claims, and the specific implementation manners described in the specification can be used to interpret the content of the claims.

Claims

1. A microbial film having the function of promoting plant growth, characterized in that: The invention comprises the following raw materials in percentage by weight: 44-48% glutinous rice flour, 6-15% seaweed powder, 5-15% polysaccharide materials, 12-17% natural cellulose, 3-4% nanoparticles, and 13-18% probiotics.

2. The microbial film having the function of promoting plant growth according to claim 1, characterized in that: The polysaccharide material is a mixture of xanthan gum and citrus pectin.

3. The microbial film having the function of promoting plant growth according to claim 1, characterized in that: The natural cellulose is one or more of wood cellulose, seed fiber and bast fiber.

4. The microbial film having the function of promoting plant growth according to claim 1, characterized in that: The nanoparticles are a mixture of silicon dioxide and zinc oxide.

5. The microbial film having the function of promoting plant growth according to claim 1, characterized in that: The probiotics are a mixture of nitrogen-fixing bacteria, phosphorus-dissolving bacteria, potassium-dissolving bacteria, bacillus and yeast.

6. A method for preparing a microbial film having the function of promoting plant growth, characterized in that: The steps include: S1. Dissolving and mixing: Add 44-48% glutinous rice flour and 5-15% polysaccharide materials to an appropriate amount of clean water, and stir evenly until a viscous solution is formed; gradually add 6-15% seaweed powder, 12-17% natural cellulose and 3-4% nanoparticles, and continue stirring to ensure that the solid materials are evenly dispersed; finally, add 13-18% probiotics, and stir gently to ensure that the probiotics are evenly distributed in the solution; S2, film formation: Pour the mixed solution of step S1 into a clean mold or tray to ensure that the liquid is flat and free of bubbles; place the mold in a low temperature environment and perform rapid freezing to ensure that the solution forms a uniform solid film; S3, freeze drying: putting the frozen membrane material into a freeze dryer for drying; S4, finishing and packaging: Check the dried film material to ensure that its thickness, uniformity and microbial survival rate meet the requirements; cut the freeze-dried microbial sustained-release film into the required shape and size; finally, package the finished product and store it in a dry, low-temperature environment to ensure the activity of the microorganisms and the performance of the film during transportation and storage.

7. The method for preparing a microbial film having the function of promoting plant growth according to claim 6, characterized in that: The freezing temperature of step S2 is set to -20°C to -40°C.

8. The method for preparing a microbial film having the function of promoting plant growth according to claim 7, characterized in that: The method of step S3 is as follows: Temperature and pressure rising stage: the frozen film material is placed in a vacuum environment, and the temperature is gradually raised to sublime the ice crystals and remove moisture; the temperature in this stage is set between -40°C and -20°C; Drying stage: Further remove residual moisture in a vacuum environment to ensure that the membrane material is completely dry and the microorganisms remain active; the drying time is 24-48 hours.

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