Chopping device for fetal brain tissue before primary culture of neural stem cells

Abstract

The invention discloses a chopping device for fetal brain tissue before primary culture of neural stem cells, and relates to the technical field of fetal brain tissue chopping, the chopping device comprises a shell, a supporting column is fixedly connected to the side wall of one end of the shell, and a supporting plate is fixedly connected to one end of the supporting column; and the side wall of the bottom end of the supporting plate is fixedly connected with a uniform chopping assembly. According to the fetal brain tissue cutting device, a side plate can drive a cutter to transversely cut and separate fetal brain tissues extruded into a cutting groove through a second opening, at the moment, the fetal brain tissues are cut into small tissue blocks with uniform sizes by the cutter in a cutting plate, air is conveyed into a cavity through an air pump, and finally, the air is blown out through an air outlet hole; when the fetal brain tissue cutting device is used, the fetal brain tissue in the cutting groove is blown downwards, the cut fetal brain tissue is prevented from remaining in the cutting groove, and the situation that when researchers cut the fetal brain tissue through ophthalmic scissors, the fetal brain tissue floats around in a culture solution, and consequently the researchers cannot cut the fetal brain tissue evenly and rapidly and effectively is avoided.

Description

Technical Field

[0001] The present invention belongs to the technical field of fetal brain tissue chopping, and particularly relates to a chopping device for fetal brain tissue before primary culture of neural stem cells. Background Art

[0002] Neural stem cells (NSCs) are a type of mother cells with the potential for division and self-renewal ability. They can produce various types of cells in neural tissue through unequal division methods. Currently, the main source of neural stem cells used in clinical research in China is the neural stem cells in embryonic tissues, and the main source of specimen collection is embryonic brain tissue.

[0003] However, before culturing neural stem cells, it is usually necessary for researchers to hold an ophthalmic scissors to chop the fetal brain tissue by hand, so as to increase the contact area between the tissue and the enzyme, make the enzyme act more fully on the extracellular matrix and intercellular connections in the tissue, thereby accelerating the dissociation process of cells and improving the cell acquisition efficiency. However, when chopping the fetal brain tissue, the culture medium and the fetal brain tissue will be in the same culture dish. At this time, when the researcher chops the fetal brain tissue, the fetal brain tissue will float around in the culture medium, resulting in the researcher being unable to quickly and effectively chop the fetal brain tissue evenly, reducing the efficiency. And after each chopping of the fetal brain tissue, it is necessary for the researcher to manually clean the ophthalmic scissors and the culture dish, which greatly reduces the chopping effect and reliability when chopping the fetal brain tissue.

[0004] Therefore, we propose a chopping device for fetal brain tissue before primary culture of neural stem cells to solve the above problems. Summary of the Invention

[0005] In order to achieve the above object, the present invention adopts the following technical scheme: A chopping device for fetal brain tissue before primary culture of neural stem cells, comprising a housing, one end side wall of the housing is fixedly connected with a support column, one end of the support column is fixedly connected with a support plate, the bottom end side wall of the support plate is fixedly connected with a uniform chopping assembly, the bottom end inner wall of the housing is fixedly connected with a first electric telescopic rod, the telescopic end of the first electric telescopic rod is fixedly connected with a first U-shaped plate, the inner wall of the first U-shaped plate is rotatably connected with a first round rod, the side wall of the first U-shaped plate is fixedly connected with a first motor, the output end of the first motor penetrates through the side wall of the first U-shaped plate and is fixedly connected with one end of the first round rod, the rod wall of the first round rod is fixedly connected with a connecting plate, the top end side wall of the connecting plate is fixedly connected with a placing plate, and the side wall of the housing is fixedly connected with an automatic cleaning assembly.

[0006] Preferably, the uniform shredding component includes a second electric telescopic rod fixedly connected to the side wall at the bottom end of the support plate. The telescopic end of the second electric telescopic rod is fixedly connected to a cutting plate. A plurality of shredding grooves are formed in the side wall at the bottom end of the cutting plate. A cavity is formed inside the cutting plate. Air outlet holes communicating with the cavity are formed in the top inner walls of the plurality of shredding grooves. Solenoid valves are arranged inside the air outlet holes. An air pump is fixedly connected to the side wall at the top end of the cutting plate. The air outlet end of the air pump extends into the cavity through the side wall of the cutting plate.

[0007] Preferably, a first groove is formed in one side wall of the cutting plate. A first electric slide rail is fixedly connected to the inner wall at one end of the first groove. A first sliding plate is slidably connected to the side wall at one end of the first electric slide rail. A plurality of side plates are fixedly connected to the side wall at one end of the first sliding plate. A plurality of first openings communicating with the shredding grooves and for the side plates to move through are formed in the inner wall of the first groove.

[0008] Preferably, a plurality of second openings are formed in one side wall of the cutting plate. The corresponding shredding grooves are communicated through the second openings. Cutting knives are fixedly connected to the side walls at one ends of the side plates. A plurality of the cutting knives are all located inside the corresponding second openings.

[0009] Preferably, the automatic cleaning component includes two fixing plates symmetrically and fixedly connected to the side walls at both ends of the housing. Second electric slide rails are fixedly connected to the inner walls of the fixing plates. Second sliding plates are slidably connected to the side walls at the top ends of the second electric slide rails. Third electric telescopic rods are fixedly connected to the side walls at the top ends of the second sliding plates. The telescopic ends of the two third electric telescopic rods are fixedly connected to the same connecting shell.

[0010] Preferably, a water pump is fixedly connected to the side wall at one end of the connecting shell. The water outlet end of the water pump extends into the connecting shell through the side wall of the connecting shell. The water inlet end of the water pump is fixedly communicated with a water inlet pipe. A plurality of first spray heads are fixedly connected to the side wall at the bottom end of the connecting shell. The water inlet ends of the first spray heads all extend into the connecting shell through the side wall of the connecting shell.

[0011] Preferably, a plurality of through holes are formed in the side wall at the top end of the connecting shell. Connecting pipes are fixedly connected inside the corresponding through holes. Second spray heads are fixedly connected to the side walls at the top ends of the connecting pipes. The water inlet ends of the second spray heads extend into the connecting pipes through the side walls of the connecting pipes. A plurality of third spray heads are fixedly connected to the outer walls of the connecting pipes. The water inlet ends of the plurality of third spray heads all extend into the corresponding connecting pipes through the side walls of the connecting pipes.

[0012] Preferably, a plurality of second U-shaped plates are fixedly connected to the outer wall of the connecting pipe. Second round rods are rotatably connected to the inner walls of the second U-shaped plates. A second motor is fixedly connected to the side wall of the second U-shaped plate. The output end of the second motor penetrates through the side wall of the second U-shaped plate and is fixedly connected to one end of the second round rod. A fourth electric telescopic rod is fixedly connected to the rod wall of the second round rod. Sleeve cases are fixedly connected to the telescopic ends of the fourth electric telescopic rods. Fourth spray heads are fixedly connected to the upper and lower side walls of each sleeve case. The water inlet ends of the fourth spray heads all penetrate through the side wall of the sleeve case and extend inwards. Through holes are formed in the side walls of the sleeve cases, and spring hoses are fixedly connected to the corresponding through holes. One end of each spring hose penetrates through the side wall of the corresponding connecting pipe and extends inwards.

[0013] Compared with the prior art, the beneficial effects of the present invention are as follows: Through the arranged uniform shredding assembly and automatic cleaning assembly, when it is necessary to shred fetal brain tissue, the side plate can drive the cutter to horizontally shred and separate the fetal brain tissue extruded into the shredding groove through the second opening. At this time, the fetal brain tissue is shredded into small tissue blocks of uniform size by the cutter inside the cutting plate. And the air pump is used to convey air into the cavity and finally blow it out through the air outlet holes to blow the fetal brain tissue in the shredding groove downwards, preventing the shredded fetal brain tissue from remaining inside the shredding groove. This avoids the situation where when researchers use ophthalmic scissors to shred fetal brain tissue, the fetal brain tissue floats around in the culture solution, resulting in the researchers being unable to quickly and effectively shred the fetal brain tissue evenly. At the same time, the first spray head, the second spray head, the third spray head and the fourth spray head can automatically clean the inner wall of the shredding groove, the inner wall of the second opening and the upper part of the placing plate, preventing the need for researchers to manually clean the ophthalmic scissors and the culture dish every time after shredding the fetal brain tissue, and greatly improving the shredding effect and reliability when shredding fetal brain tissue. BRIEF DESCRIPTION OF THE DRAWINGS

[0014] Figure 1 is a schematic diagram of the overall structure of the present invention; Figure 2 is a schematic diagram of the structure of the present invention from other angles; Figure 3 is a schematic cross-sectional view of a part of the structure of the present invention; Figure 4 is a schematic diagram of a part of the structure of the present invention Figure 1 ; Figure 5 is an enlarged view of part A of FIG. 4 of the present invention; Figure 6 is a schematic diagram of a part of the structure of the present invention Figure 2 ; Figure 7 is an enlarged view of part B of FIG. 6 of the present invention; Figure 8 is a schematic diagram of a part of the structure of the present invention Figure 3; Figure 9 Schematic diagram of part of the structure of the present invention Figure 4 .

[0015] In the figure: 1. Outer shell; 2. Support column; 3. Support plate; 4. Uniform shredding assembly; 41. Second electric telescopic rod; 42. Cutting plate; 43. Shredding groove; 45. Air outlet; 46. Solenoid valve; 47. Air pump; 48. First groove; 49. First electric slide rail; 410. First slide plate; 411. Side plate; 412. First opening; 413. Second opening; 414. Cutter; 5. First electric telescopic rod; 6. First U-shaped plate; 7. First round rod; 8. First motor; 9. Connecting plate; 10. Placing plate; 11. Automatic cleaning assembly; 111. Fixed plate; 112. Second electric slide rail; 113. Second slide plate; 114. Third electric telescopic rod; 115. Connecting shell; 116. Water pump; 117. Water inlet pipe; 118. First spray head; 119. Connecting pipe; 1110. Second spray head; 1111. Third spray head; 1114. Second U-shaped plate; 1115. Second round rod; 1116. Second motor; 1117. Fourth electric telescopic rod; 1118. Sleeve; 1119. Fourth spray head; 1120. Spring hose. Specific embodiments

[0016] Next, the technical solutions in the embodiments of the present invention will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments.

[0017] The following electrical components are all electrically connected to the external PLC controller.

[0018] Refer to Figure 1 - Figure 9 , a shredding device for fetal brain tissue before primary culture of neural stem cells, including an outer shell 1, a support column 2 is fixedly connected to one end side wall of the outer shell 1, a support plate 3 is fixedly connected to one end of the support column 2, a uniform shredding assembly 4 is fixedly connected to the bottom end side wall of the support plate 3, a first electric telescopic rod 5 is fixedly connected to the bottom end inner wall of the outer shell 1, a first U-shaped plate 6 is fixedly connected to the telescopic end of the first electric telescopic rod 5, a first round rod 7 is rotatably connected to the inner wall of the first U-shaped plate 6, a first motor 8 is fixedly connected to the side wall of the first U-shaped plate 6, the output end of the first motor 8 penetrates the side wall of the first U-shaped plate 6 and is fixedly connected to one end of the first round rod 7, a connecting plate 9 is fixedly connected to the rod wall of the first round rod 7, a placing plate 10 is fixedly connected to the top end side wall of the connecting plate 9, and an automatic cleaning assembly 11 is fixedly connected to the side wall of the outer shell 1.

[0019] In the embodiment, the uniform chopping assembly 4 includes a second electric telescopic rod 41 fixedly connected to the side wall at the bottom end of the support plate 3. The telescopic end of the second electric telescopic rod 41 is fixedly connected with a cutting plate 42. A plurality of chopping grooves 43 are formed in the side wall at the bottom end of the cutting plate 42. A cavity is formed inside the cutting plate 42. Air outlet holes 45 communicating with the cavity are formed in the top inner walls of the plurality of chopping grooves 43. Solenoid valves 46 are arranged inside the air outlet holes 45. An air pump 47 is fixedly connected to the side wall at the top end of the cutting plate 42. The air outlet end of the air pump 47 penetrates through the side wall of the cutting plate 42 and extends into the cavity. A first groove 48 is formed in one side wall of the cutting plate 42. A first electric slide rail 49 is fixedly connected to the inner wall at one end of the first groove 48. A first sliding plate 410 is slidably connected to the side wall at one end of the first electric slide rail 49. A plurality of side plates 411 are fixedly connected to the side wall at one end of the first sliding plate 410. A plurality of first openings 412 communicating with the chopping grooves 43 and for the side plates 411 to move are formed in the inner wall of the first groove 48. A plurality of second openings 413 are formed in one side wall of the cutting plate 42. The corresponding chopping grooves 43 are communicated through the second openings 413. Cutting knives 414 are fixedly connected to the side walls at one ends of the side plates 411. A plurality of cutting knives 414 are all located inside the corresponding second openings 413.

[0020] Specifically, when it is necessary to chop the fetal brain tissue, the side plates 411 can drive the cutting knives 414 to horizontally chop and separate the fetal brain tissue extruded into the chopping grooves 43 through the second openings 413. At this time, the fetal brain tissue is chopped into small tissue blocks of uniform size by the cutting knives 414 inside the cutting plate 42. The air pump 47 is used to convey air into the cavity and finally blown out through the air outlet holes 45 to blow the fetal brain tissue in the chopping grooves 43 downward, preventing the chopped fetal brain tissue from remaining inside the chopping grooves 43, and avoiding the situation that when the researcher uses an ophthalmic scissors to chop the fetal brain tissue, the fetal brain tissue floats around in the culture solution, resulting in the researcher being unable to quickly and effectively chop the fetal brain tissue evenly.

[0021] In the embodiment, the automatic cleaning assembly 11 includes two fixing plates 111 symmetrically and fixedly connected to the side walls at both ends of the housing 1. Second electric slide rails 112 are fixedly connected to the inner walls of the fixing plates 111. Second sliding plates 113 are slidably connected to the side walls at the top ends of the second electric slide rails 112. Third electric telescopic rods 114 are fixedly connected to the side walls at the top ends of the second sliding plates 113. The telescopic ends of the two third electric telescopic rods 114 are fixedly connected to the same connection shell 115. A water pump 116 is fixedly connected to one side wall of the connection shell 115. The water outlet end of the water pump 116 penetrates through the side wall of the connection shell 115 and extends inward. The water inlet end of the water pump 116 is fixedly communicated with a water inlet pipe 117. A plurality of first spray heads 118 are fixedly connected to the side wall at the bottom end of the connection shell 115. The water inlet ends of the first spray heads 118 all penetrate through the side wall of the connection shell 115 and extend inward. A plurality of through holes are formed in the top side wall of the connecting shell 115, and a connecting pipe 119 is fixedly connected inside each corresponding through hole. A second spray head 1110 is fixedly connected to the top side wall of the connecting pipe 119. The water inlet end of the second spray head 1110 extends inward through the side wall of the connecting pipe 119. A plurality of third spray heads 1111 are fixedly connected to the outer wall of the connecting pipe 119. The water inlet ends of the plurality of third spray heads 1111 all extend inward through the side wall of the corresponding connecting pipe 119. A plurality of second U-shaped plates 1114 are fixedly connected to the outer wall of the connecting pipe 119. A second round rod 1115 is rotatably connected to the inner wall of each second U-shaped plate 1114. A second motor 1116 is fixedly connected to the side wall of the second U-shaped plate 1114. The output end of the second motor 1116 extends through the side wall of the second U-shaped plate 1114 and is fixedly connected to one end of the second round rod 1115. A fourth electric telescopic rod 1117 is fixedly connected to the rod wall of the second round rod 1115. The telescopic ends of the fourth electric telescopic rods 1117 are all fixedly connected with a sleeve 1118. Fourth spray heads 1119 are fixedly connected to the upper and lower side walls of the sleeve 1118. The water inlet ends of the fourth spray heads 1119 all extend inward through the side wall of the sleeve 1118. Through holes are formed in the side walls of the sleeves 1118, and a spring hose 1120 is fixedly connected inside each corresponding through hole. One end of the spring hose 1120 extends inward through the side wall of the corresponding connecting pipe 119.

[0022] Specifically, the first spray head 118, the second spray head 1110, the third spray head 1111 and the fourth spray head 1119 can be used to automatically clean the inner wall of the chopping groove 43, the inner wall of the second opening 413 and the upper part of the placing plate 10, preventing the need for researchers to manually clean the ophthalmic scissors and the culture dish every time the fetal brain tissue is chopped, greatly improving the chopping effect and reliability when chopping the fetal brain tissue.

[0023] The operating principle of the present invention is described as follows: In the present invention, when it is necessary to chop the fetal brain tissue, the researcher puts the fetal brain tissue into the holding plate 10, then pours the corresponding dose of culture solution, and then controls the second electric telescopic rod 41 to start, driving the cutting plate 42 to move in the direction of the fetal brain tissue. After the cutting plate 42 contacts the inner wall of the top of the holding plate 10, the fetal brain tissue will be squeezed into the plurality of chopping grooves 43, completing the longitudinal chopping and separation of the fetal brain tissue. Then, control the first electric slide rail 49 to start, drive the first slide plate 410 to move, and use the first slide plate 410 to drive the side plate 411 to move, so that the side plate 411 drives the cutter 414 to horizontally chop and separate the fetal brain tissue squeezed into the chopping groove 43 through the second opening 413. At this time, the fetal brain tissue is chopped into small tissue blocks of uniform size by the cutter 414 inside the cutting plate 42. Then, control the second electric telescopic rod 41 to start, drive the cutting plate 42 to move upward, so that the chopped fetal brain tissue falls back above the holding plate 10 from the chopping groove 43. During this process, control the air pump 47 and the plurality of electromagnetic valves 46 to start, use the air pump 47 to transport air into the cavity, and finally blow it out through the air outlet 45 to blow the fetal brain tissue in the chopping groove 43 downward, preventing the chopped fetal brain tissue from remaining inside the chopping groove 43. After all the fetal brain tissue in the chopping groove 43 has fallen above the holding plate 10, the chopping of the fetal brain tissue is completed, which is convenient for accelerating the cell dissociation process and improving the cell acquisition efficiency. When it is necessary to take out the chopped fetal brain tissue from above the holding plate 10, control the first electric telescopic rod 5 to start, drive the holding plate 10 to move upward, so that the holding plate 10 is located outside the housing 1. Then, control the first motor 8 to start, drive the first round rod 7 to rotate, so that the first round rod 7 drives the connecting plate 9 to rotate, thereby driving the holding plate 10 to rotate in the direction of the side opening. At this time, the researcher places the container below the side opening of the holding plate 10, and the culture solution and fetal brain tissue in the holding plate 10 will flow into the container, completing the rapid collection of the fetal brain tissue. Then, connect the external water pipe to the water inlet pipe 117, and then control the water pump 116 to start, use the water pump 116 to transport water through the water inlet pipe 117 into the connection shell 115. Then, control the third electric telescopic rod 114 to start, drive the connection shell 115 to move upward, so that the connection shell 115 is located above the housing 1. Then, control the second electric slide rail 112 to start, drive the second slide plate 113 to move, so that the connection shell 115 moves in the direction of the cutting plate 42. When the connecting pipe 119 is located below the leftmost column of chopping grooves 43, control the second electric slide rail 112 to close. Then, control the third electric telescopic rod 114 to start, drive the connection shell 115 and the connecting pipe 119 to move upward, so that the connecting pipe 119 is inserted into the corresponding chopping groove 43. Then, control the second spray head 1110 and the third spray head 1111 to start, transport the water in the connection shell 115 into the connecting pipe 119, and then spray the water onto the inner wall of the chopping groove 43 through the second spray head 1110 and the third spray head 1111 to clean the inner wall of the chopping groove 43. At the same time, control the fourth electric telescopic rod 1117 to start,Drive the sleeve 1118 to move, so that the sleeve 1118 moves into the corresponding second opening 413. Then, control the fourth nozzle 1119 to start and spray water on the inner wall of the second opening 413, thereby cleaning the inner wall of the second opening 413. At the same time, control the second motor 1116 to start, drive the second round rod 1115 to rotate, and make the sleeve 1118 move in the second opening 413. During this process, control the fourth electric telescopic rod 1117 to continuously expand and contract to ensure that the sleeve 1118 can always be located in the second opening 413, which is convenient for cleaning the second opening 413. After cleaning this row of chopping grooves 43, control the second electric slide rail 112 and the third electric telescopic rod 114 to start, so that the connecting pipe 119 enters other chopping grooves 43 for cleaning. After cleaning all the chopping grooves 43, the residual fetal brain tissue and water in the chopping grooves 43 will fall above the placing plate 10 at this time. Then, control the second electric slide rail 112 to drive the second slide plate 113 to return to its original position. During this process, control the first nozzle 118 to start and spray water above the placing plate 10, thereby cleaning the placing plate 10. During this process, control the placing plate 10 to move out of the housing 1 and make the placing plate 10 in an inclined state. Under the flushing of the first nozzle 118, the water and fetal brain tissue in the placing plate 10 will flow out of the placing plate 10. At this time, the cleaning of the cutting plate 42 and the placing plate 10 is completed. When it is necessary to chop the fetal brain tissue, the side plate 411 can drive the cutter 414 to horizontally chop and separate the fetal brain tissue extruded into the chopping groove 43 through the second opening 413. At this time, the fetal brain tissue is chopped into small tissue blocks of uniform size by the cutter 414 inside the cutting plate 42. And use the air pump 47 to transport air into the cavity and finally blow it out through the air outlet 45 to blow the fetal brain tissue in the chopping groove 43 downward, preventing the chopped fetal brain tissue from remaining inside the chopping groove 43, and avoiding the situation that when the researcher uses the ophthalmic scissors to chop the fetal brain tissue, the fetal brain tissue floats around in the culture solution, resulting in the researcher being unable to quickly and effectively chop the fetal brain tissue evenly. At the same time, the inner wall of the chopping groove 43, the inner wall of the second opening 413 and the area above the placing plate 10 can be automatically cleaned by the first nozzle 118, the second nozzle 1110, the third nozzle 1111 and the fourth nozzle 1119, preventing the need for the researcher to manually clean the ophthalmic scissors and the culture dish every time after chopping the fetal brain tissue, which greatly improves the chopping effect and reliability when chopping the fetal brain tissue.

[0024] The above is only a preferred specific embodiment of the present invention, but the protection scope of the present invention is not limited thereto. Any person skilled in the art within the technical scope disclosed by the present invention, according to the technical solution and inventive concept of the present invention, makes equivalent substitutions or changes, and all should be covered within the protection scope of the present invention.

Claims

1. A chopping device for prenatal brain tissue for primary culture of neural stem cells, comprising a housing (1), characterized in that: A support column (2) is fixedly connected to a side wall at one end of the housing (1); a support plate (3) is fixedly connected to one end of the support column (2); a uniform chopping assembly (4) is fixedly connected to the side wall at the bottom end of the support plate (3); a first electric telescopic rod (5) is fixedly connected to the inner wall at the bottom end of the housing (1); a first U-plate (6) is fixedly connected to the telescopic end of the first electric telescopic rod (5); a first round rod (7) is rotatably connected to the inner wall of the first U-plate (6); a first motor (8) is fixedly connected to the side wall of the first U-plate (6); an output end of the first motor (8) passes through the side wall of the first U-plate (6) and is fixedly connected to one end of the first round rod (7); a connecting plate (9) is fixedly connected to the rod wall of the first round rod (7); a holding plate (10) is fixedly connected to the top side wall of the connecting plate (9); and an automatic cleaning assembly (11) is fixedly connected to the side wall of the housing (1).

2. The chopping device for fetal brain tissue before primary culture of neural stem cells according to claim 1, characterized in that: The uniform chopping assembly (4) comprises a second electric telescopic rod (41) fixedly connected to the side wall at the bottom end of the support plate (3); the telescopic end of the second electric telescopic rod (41) is fixedly connected to a cutting plate (42); the side wall at the bottom end of the cutting plate (42) is provided with a plurality of chopping grooves (43); a cavity is provided inside the cutting plate (42); the top inner walls of the plurality of chopping grooves (43) are provided with air outlet holes (45) communicating with the cavity; the air outlet holes (45) are provided with electromagnetic valves (46); the top side wall of the cutting plate (42) is fixedly connected to an air pump (47); the air outlet end of the air pump (47) penetrates the side wall of the cutting plate (42) and protrudes into the cavity.

3. The chopping device for fetal brain tissue before primary culture of neural stem cells according to claim 2, characterized in that: A first groove (48) is formed on a side wall at one end of the cutting plate (42); a first electric slide rail (49) is fixedly connected to an inner wall at one end of the first groove (48); a first slide plate (410) is slidably connected to a side wall at one end of the first electric slide rail (49); a plurality of side plates (411) are fixedly connected to a side wall at one end of the first slide plate (410); and a plurality of first openings (412) are formed on an inner wall of the first groove (48) and are communicated with the chopping groove (43) and are provided for the side plates (411) to move.

4. The chopping device for fetal brain tissue before primary culture of neural stem cells according to claim 3, characterized in that: A plurality of second openings (413) are formed on a side wall at one end of the cutting plate (42), and the second openings (413) connect the corresponding shredding grooves (43). A cutting knife (414) is fixedly connected to the side wall at one end of the side plate (411), and the plurality of cutting knives (414) are located inside the corresponding second openings (413).

5. The chopping device for fetal brain tissue before primary culture of neural stem cells according to claim 1, characterized in that: The automatic cleaning component (11) comprises two fixed plates (111) symmetrically fixedly connected to the side walls at both ends of the housing (1); the inner walls of the fixed plates (111) are fixedly connected to second electric slide rails (112); the top side walls of the second electric slide rails (112) are slidably connected to second slide plates (113); the top side walls of the second slide plates (113) are fixedly connected to third electric telescopic rods (114); and the telescopic ends of the two third electric telescopic rods (114) are fixedly connected to the same connecting shell (115).

6. The chopping device for fetal brain tissue before primary culture of neural stem cells according to claim 5, characterized in that: A water pump (116) is fixedly connected to a side wall at one end of the connection shell (115); a water outlet end of the water pump (116) penetrates the side wall of the connection shell (115) and protrudes inwardly; a water inlet end of the water pump (116) is fixedly connected to a water inlet pipe (117); a plurality of first nozzles (118) are fixedly connected to the side wall at the bottom end of the connection shell (115); water inlet ends of the first nozzles (118) all penetrate the side wall of the connection shell (115) and protrude inwardly.

7. The chopping device for fetal brain tissue before primary culture of neural stem cells according to claim 6, characterized in that: The top side wall of the connecting shell (115) is provided with a plurality of through holes, and the corresponding through holes are all fixedly connected to a connecting pipe (119), the top side wall of the connecting pipe (119) is fixedly connected to a second nozzle (1110), the water inlet end of the second nozzle (1110) penetrates the side wall of the connecting pipe (119) and protrudes inwardly, and the outer wall of the connecting pipe (119) is fixedly connected to a plurality of third nozzles (1111), the water inlet ends of the plurality of third nozzles (1111) penetrate the side wall of the corresponding connecting pipe (119) and protrude inwardly.

8. The chopping device for fetal brain tissue before primary culture of neural stem cells according to claim 7, characterized in that: The outer wall of the connecting tube (119) is fixedly connected to a plurality of second U-plates (1114); the inner walls of the second U-plates (1114) are rotatably connected to second round rods (1115); the side walls of the second U-plates (1114) are fixedly connected to a second motor (1116); the output end of the second motor (1116) passes through the side wall of the second U-plate (1114) and is fixedly connected to one end of the second round rod (1115); the rod wall of the second round rod (1115) is fixedly connected to a fourth electric telescopic rod (1117); The telescopic ends of the fourth electric telescopic rod (1117) are fixedly connected to a casing (1118), and the upper and lower side walls of the casing (1118) are fixedly connected to a fourth nozzle (1119), and the water inlet end of the fourth nozzle (1119) penetrates the side wall of the casing (1118) and protrudes inwardly. The side wall of the casing (1118) is provided with a through hole, and a spring hose (1120) is fixedly connected to the inside of the corresponding through hole, and one end of the spring hose (1120) penetrates the side wall of the corresponding connecting pipe (119) and protrudes inwardly.

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