Cell imaging chip, imaging system, cell detection system and detection method

Abstract

The invention relates to the technical field of cells, and provides a cell imaging chip, an imaging system, a cell detection system and a detection method. The chip comprises a layer plate and an imaging plate, the imaging plate is arranged at the bottom of the layer plate, and a gap layer is arranged between the imaging plate and the layer plate; the laminated plate is provided with a flow guide flow channel, the flow guide flow channel comprises a liquid inlet flow channel and at least one flow guide groove, and the flow guide groove is respectively communicated with the liquid inlet flow channel and the gap layer, so that cell imaging is carried out after a cell sample flowing into the flow guide groove from the liquid inlet flow channel is guided to the gap layer. According to the cell imaging chip provided by the invention, miniaturization, low cost and easy operation of cell detection equipment are possible, and the cell imaging chip can be used for carrying out instant cell detection in common consulting rooms, remote rural hospitals and even families.

Description

Technical Field

[0001] This application relates to the field of cell technology, and particularly to a cell imaging chip, an imaging system, a cell detection system and a detection method. Background Art

[0002] Among many cell detection means, classifying, counting and morphological analysis of cells are the basis of cell detection. Currently, the more widely used devices are flow cytometers and optical microscopes. However, these devices have common problems, such as large volume, high price, complex operation, etc., which makes them generally only configured in large hospitals or large laboratories with conditions.

[0003] With the improvement of living standards, people also have a need for professional cell detection. Environmental detection, health detection, and medical diagnosis all require miniaturization and instantaneity of cell detection devices, while the existing cell detection devices cannot meet such needs. Summary of the Invention

[0004] Embodiments of this application provide a cell imaging chip, an imaging system, a cell detection system and a detection method to solve the technical problem that existing cell detection devices cannot meet the requirements of miniaturization and instantaneity of cell detection devices.

[0005] In a first aspect, embodiments of this application provide a cell imaging chip, including: a laminate and an imaging plate;

[0006] The imaging plate is arranged at the bottom of the laminate, and a gap layer is arranged between the imaging plate and the laminate;

[0007] A diversion channel is arranged on the laminate, and the diversion channel includes a liquid inlet channel and at least one diversion groove. The diversion grooves are respectively communicated with the liquid inlet channel and the gap layer to divert the cell sample flowing into the diversion groove from the liquid inlet channel to the gap layer for cell imaging.

[0008] In an embodiment, the diversion channel further includes a liquid outlet channel, and the liquid outlet channel is arranged on the opposite side of the liquid inlet channel;

[0009] The diversion grooves are evenly distributed on both sides of the liquid outlet channel and the liquid inlet channel, and the diversion groove includes a first extension groove and a second extension groove;

[0010] The first extension groove extends towards the center of the chip, and the first extension groove is isolated from the gap layer. The second extension groove has a different extension direction from the first extension groove, and the second extension groove is communicated with the gap layer.

[0011] In one embodiment, the first diversion groove on the side of the liquid inlet flow channel is arranged at the first bottom on the side of the liquid inlet flow channel away from the liquid inlet, and one end of the first extension groove of the first diversion groove communicates with the first bottom, and the other end of the first extension groove of the first diversion groove communicates with the second extension groove of the first diversion groove.

[0012] In one embodiment, the second diversion groove on the side of the liquid outlet flow channel is arranged at the second bottom on the side of the liquid outlet flow channel away from the liquid outlet, and one end of the first extension groove of the second diversion groove communicates with the second bottom, and the other end of the first extension groove of the second diversion groove communicates with the second extension groove of the second diversion groove.

[0013] In one embodiment, a groove is arranged at the center position of the top of the lamina, and an observation window is arranged at the bottom of the groove for observing the cell sample in the gap layer and providing a shooting position for cell imaging.

[0014] In a second aspect, an embodiment of the present application provides a cell imaging system, including: an imaging device and the cell imaging chip described in the first aspect;

[0015] The imaging device is arranged on the perpendicular line of the plane where the gap layer of the cell imaging chip is located, and is used for photographing the cell sample in the gap layer.

[0016] In a third aspect, an embodiment of the present application provides a cell detection system, including: a sampling system, a first driving device, an optical detection system, a data processing and analysis system, and the cell imaging system described in the second aspect;

[0017] The output end of the sampling system is connected to the output end of the cell imaging system, and the first driving device is connected between the sampling system and the cell imaging system for transporting the cell sample in the sampling system to the cell imaging system;

[0018] The cell imaging system is arranged in the optical detection system, and the output end of the optical detection system is connected to the input end of the data processing and analysis system;

[0019] The optical detection system is used for performing high-definition processing on the cell image output by the cell imaging system to obtain a high-definition image, and transmitting the high-definition image to the data processing and analysis system;

[0020] The data processing and analysis system is used for performing cell detection on the high-definition image.

[0021] In one embodiment, it further includes: a second driving device and a sample output system;

[0022] The output end of the cell imaging system is connected to the input end of the sample output system, and the second driving device is connected between the cell imaging system and the sample output system for transporting the cell sample that has completed cell imaging in the cell imaging system to the sample output system.

[0023] Fourthly, an embodiment of the present application provides a cell detection method implemented by using the cell detection system described in the third aspect, including: controlling the first driving device to transport the cell sample in the sample input system to the cell imaging system;

[0024] Controlling the cell imaging system to image the cell sample to obtain a cell image;

[0025] Controlling the optical detection system to perform high-definition processing on the cell image to obtain a high-definition image;

[0026] Controlling the optical detection system to transmit the high-definition image to the data processing and analysis system;

[0027] Controlling the data processing and analysis system to perform cell detection on the high-definition image.

[0028] In one embodiment, the controlling the cell imaging system to image the cell sample to obtain a cell image includes:

[0029] Controlling the imaging device in the cell imaging system to image the cell sample in the gap layer at the observation window to obtain a cell image.

[0030] The cell imaging chip provided by the present application includes a layer board and an imaging board. The imaging board is arranged at the bottom of the layer board, and a gap layer is arranged between the imaging board and the layer board. A diversion flow channel is arranged on the layer board. The diversion flow channel includes an inlet flow channel and at least one diversion groove. The diversion grooves are respectively communicated with the inlet flow channel and the gap layer to divert the cell sample flowing into the diversion grooves from the inlet flow channel to the gap layer for cell imaging after that. After obtaining cell imaging through the cell imaging chip, cell detection activities such as cell classification, counting, and morphological analysis can be carried out by analyzing the cell imaging. Since the cell imaging chip is small in volume and low in cost and can be integrated into the cell detection device, it makes it possible for the cell detection device to be miniaturized, low-cost, and easy to operate, and can be used for instant cell detection in ordinary consulting rooms, remote rural hospitals, and even at home. Description of the Drawings

[0031] To more clearly illustrate the technical solutions in the present application or the prior art, the following will briefly introduce the accompanying drawings required for the description of the embodiments or the prior art. Obviously, the accompanying drawings in the following description are some embodiments of the present application. For those of ordinary skill in the art, without creative efforts, other accompanying drawings can also be obtained based on these drawings.

[0032] Figure 1 It is one of the structural diagrams of the cell imaging chip provided by the embodiments of the present application;

[0033] Figure 2 It is the second structural diagram of the cell imaging chip provided by the embodiments of the present application;

[0034] Figure 3 is Figure 2 the enlarged view of the partial A of

[0035] Figure 4 It is the structural diagram of the cell imaging system provided by the embodiments of the present application;

[0036] Figure 5 It is the schematic diagram of the cell detection system provided by the embodiments of the present application;

[0037] Figure 6 It is the schematic flow diagram of the cell detection method provided by the embodiments of the present application;

[0038] Figure 7 It is the structural diagram of the electronic device provided by the embodiments of the present application.

[0039] Reference numerals:

[0040] 1 - laminate; 2 - imaging plate; 3 - slit layer; 4 - liquid inlet channel; 5 - diversion groove; 51 - first extension groove; 52 - second extension groove; 6 - liquid outlet channel; 7 - groove; 8 - imaging device. Detailed implementation manners

[0041] To make the objectives, technical solutions, and advantages of the present application clearer, the following will clearly and completely describe the technical solutions in the present application with reference to the accompanying drawings in the embodiments of the present application. Obviously, the described embodiments are some, but not all, of the embodiments of the present application. All other embodiments obtained by those of ordinary skill in the art without creative efforts based on the embodiments in the present application belong to the scope of protection of the present application.

[0042] Figure 1 It is one of the structural diagrams of the cell imaging chip provided by the embodiments of the present application;

[0043] Figure 2 It is the second structural diagram of the cell imaging chip provided by the embodiments of the present application;

[0044] Figure 3 is Figure 2 a magnified view of partial A of

[0045] Referring to Figures 1 to 3 , an embodiment of the present application provides a cell imaging chip, which may include: a laminar plate 1 and an imaging plate 2;

[0046] The imaging plate 2 is disposed at the bottom of the laminar plate 1, and a gap layer 3 is disposed between the imaging plate 2 and the laminar plate 1;

[0047] A diversion channel is disposed on the laminar plate 1. The diversion channel includes a liquid inlet channel 4 and at least one diversion groove 5. The diversion grooves 5 are respectively communicated with the liquid inlet channel 4 and the gap layer 3 to divert the cell sample flowing into the diversion groove 5 from the liquid inlet channel 4 to the gap layer 3 and then perform cell imaging.

[0048] The cell imaging chip may be a microfluidic chip, and the microfluidic chip adopts an integrated molding technology, which includes but is not limited to 3D printing technology, CNC numerical control precision carving technology, etc. The molding of the microfluidic imaging chip can be achieved without using layer-by-layer bonding. The thickness of the gap layer 3 can be set according to actual situations and is not limited herein. In this embodiment, the thickness of the gap layer 3 can be set to 100 microns. The gap layer 3 forms a micron-scale and relatively closed cell imaging area, which can ensure that the cell sample is in a refined and controllable sterile environment.

[0049] In addition, the selection of the chip material is also diverse, including but not limited to materials such as acrylic plates and quartz glass. That is, the imaging plate in the chip can also be selected from materials such as acrylic plates or quartz glass, which is not limited herein. In this embodiment, the imaging plate adopts quartz glass material.

[0050] The cell imaging chip provided in this embodiment includes a laminar plate and an imaging plate. The imaging plate adopts quartz glass material and is disposed at the bottom of the laminar plate. A gap layer is disposed between the imaging plate and the laminar plate. A diversion channel is disposed on the laminar plate. The diversion channel includes a liquid inlet channel and at least one diversion groove. The diversion grooves are respectively communicated with the liquid inlet channel and the gap layer to divert the cell sample flowing into the diversion groove from the liquid inlet channel to the gap layer and then perform cell imaging. After obtaining cell imaging through the cell imaging chip, cell detection activities such as cell classification, counting, and morphological analysis can be performed by analyzing the cell imaging. Since the cell imaging chip is small in volume and low in cost and can be integrated into cell detection equipment, it makes it possible for cell detection equipment to be miniaturized, low-cost, and easy to operate, and can be used for instant cell detection in ordinary consulting rooms, remote rural hospitals, and even at home.

[0051] Furthermore, due to the presence of the diversion channels, the cell samples in the liquid inlet channel can be diverted, enabling the cell samples to be distributed as evenly as possible in the gap layer. As a result, each cell in the cell sample is distributed as evenly and dispersedly as possible in the gap layer, avoiding mutual occlusion caused by cell stacking and aggregation. Therefore, cell imaging pictures with good cell uniformity, non-adherent cell images, neat cell arrangement, and high cell imaging clarity can be obtained, improving the accuracy of cell detection.

[0052] Referring to Figures 1 to 3 , in one embodiment, the diversion channel further includes an outlet channel 6, and the outlet channel 6 is arranged on the side opposite to the inlet channel 4;

[0053] The diversion grooves 5 are evenly distributed on both sides of the outlet channel 6 and the inlet channel 4, and the diversion grooves 5 include a first extension groove 51 and a second extension groove 52;

[0054] The first extension groove 51 extends towards the center of the chip and is isolated from the gap layer 3. The second extension groove 52 has a different extension direction from that of the first extension groove 51 and is in communication with the gap layer 3.

[0055] The shape of the diversion groove 5 can be set according to actual needs, such as T-shaped, Y-shaped, triangular, etc., and is not limited here. In this embodiment, the shape of the diversion groove 5 can be set as T-shaped.

[0056] The first extension groove 51 of the diversion groove 5 is isolated from the gap layer 3 and extends towards the center of the chip, for transporting the cell samples in the inlet channel 4 towards the chip as much as possible, rather than flowing into the gap layer 3 during the transportation process and thus aggregating in the area near the inlet channel 4. The second extension groove 52 of the diversion groove 5 is perpendicular to the first extension groove 51 and is in communication with the gap layer 3, for diffusing the cell samples diverted by the first diversion groove 51 in a completely different direction. At this time, the cell samples entering the gap layer 3 can be diffused to a larger area, thereby achieving uniform distribution in the gap layer 3.

[0057] Furthermore, by setting the outlet channel 6 and its corresponding diversion grooves, the cell samples that have completed cell imaging in the gap layer 3 can be evenly diverted to the outlet of the outlet channel 6 by the diversion grooves, forming a cell sample flow channel of the inlet channel 4, the diversion grooves, the gap layer 3, the diversion grooves, and the outlet channel 6, ensuring that the cell samples can flow continuously without interruption, and realizing real-time continuous imaging of large-throughput, large-volume, and high-concentration cell samples.

[0058] In this embodiment, through the setting of the diversion groove structure, the cell sample can be first transported towards the center of the chip, then diffused in different directions, and at the same time, the cell sample is diverted to the gap layer to diffuse in a farther and larger range, realizing the uniform distribution of the cell sample in the gap layer. At the same time, by forming a cell sample flow channel of the liquid inlet channel, the diversion groove, the gap layer, the diversion groove, and the liquid outlet channel, it can ensure that the cell sample can flow continuously without interruption, realizing real-time continuous imaging of large-throughput, large-volume, and high-concentration cell samples, and further realizing real-time continuous detection of such cell samples, improving the stability and accuracy of the detection.

[0059] Referring to Figures 1 to 3 , in one embodiment, the first diversion groove on the side of the liquid inlet channel 4 is arranged at the first bottom of the liquid inlet channel 4 away from the liquid inlet, and one end of the first extension groove of the first diversion groove is communicated with the first bottom, and the other end of the first extension groove of the first diversion groove is communicated with the second extension groove of the first diversion groove;

[0060] Similarly, the second diversion groove on the side of the liquid outlet channel 6 is arranged at the second bottom of the liquid outlet channel 6 away from the liquid outlet, and one end of the first extension groove of the second diversion groove is communicated with the second bottom, and the other end of the first extension groove of the second diversion groove is communicated with the second extension groove of the second diversion groove.

[0061] In this embodiment, by arranging the first diversion groove at the first bottom of the liquid inlet channel, a descending step can be formed between the liquid inlet channel and the first diversion groove, and this step can slow down the flow rate of the cell sample in the liquid inlet channel, enabling the first diversion groove to divert the cell sample more smoothly, so as to spread the cell sample more evenly into the gap layer; similarly, by arranging the second diversion groove at the second bottom of the liquid outlet channel, an ascending step can be formed between the second diversion groove and the liquid outlet channel, and this step can slow down the flow rate of the cell sample in the gap layer, preventing the cell sample in the gap layer from flowing out of the chip quickly, thus ensuring the full diffusion of the cell sample in the gap layer.

[0062] Referring to Figures 1 to 2 , in one embodiment, a groove 7 is arranged at the center position of the top of the layer plate 1, and an observation window is arranged at the bottom of the groove 7 for observing the cell sample in the gap layer 3 and providing a shooting position for cell imaging.

[0063] The shape of the groove 7 can be set according to the actual situation, such as circular, rectangular, triangular, etc., which is not limited here, and the size of the groove 7 can also be set according to the actual situation, which is not limited here either. In this embodiment, the groove 7 is set as circular, the diameter of the groove is set between 16 mm and 20 mm, and the depth of the groove 7 is set between 5 mm and 6 mm.

[0064] In this embodiment, by providing a groove at the center of the top of the laminate and an observation window at its bottom, the diffusion and distribution of cell samples in the gap layer can be effectively monitored, which helps to make timely adjustments when abnormalities are found and ensure the image quality of the cell samples captured at the observation window.

[0065] Figure 4 is a structural diagram of the cell imaging system provided by an embodiment of the present application; referring to Figures 1 to 4 , an embodiment of the present application provides a cell imaging system, which may include: an imaging device 8 and the aforementioned cell imaging chip;

[0066] The imaging device 8 is arranged on the perpendicular line of the plane where the gap layer 3 of the cell imaging chip is located, and is used to capture cell samples in the gap layer 3.

[0067] The imaging device 8 can be selected according to actual situations, such as an industrial camera, a microscope, etc., which is not limited here. The setting method of the imaging device 8 can be adaptively adjusted according to the setting method of the cell imaging chip. For example, when the groove 7 of the cell imaging chip opens upward, the imaging device 8 is arranged directly above the observation window; when the groove 7 of the cell imaging chip opens downward, the imaging device 8 is arranged directly below the observation window.

[0068] The cell imaging system provided by this embodiment includes a cell imaging chip and an imaging device. After the imaging device images the cell samples in the gap layer of the cell imaging chip, cell detection activities such as cell classification, counting, and morphological analysis can be carried out by analyzing the cell imaging. Since the cell imaging system is small in size and low in cost and can be integrated into cell detection equipment, it makes it possible for cell detection equipment to be miniaturized, low-cost, and easy to operate, and can be used for instant cell detection in ordinary consulting rooms, remote rural hospitals, or even at home.

[0069] Furthermore, due to the existence of the diversion groove, the cell samples in the liquid inlet channel can be diverted, so that the cell samples are distributed as evenly as possible in the gap layer. Furthermore, each cell in the cell samples is distributed as evenly and dispersedly as possible in the gap layer, avoiding mutual occlusion caused by cell stacking and aggregation. Therefore, the imaging device can capture cell imaging pictures with good cell uniformity, non-adhesive cell images, neat cell arrangement, and high cell imaging clarity, improving the accuracy of cell detection.

[0070] Figure 5 is a schematic diagram of the cell detection system provided by an embodiment of the present application; referring to Figure 5 , an embodiment of the present application provides a cell detection system, which may include: a sampling system, a first driving device, an optical detection system, a data processing and analysis system, and the aforementioned cell imaging system;

[0071] The output end of the sample injection system is connected to the output end of the cell imaging system. A first driving device is connected between the sample injection system and the cell imaging system for transporting the cell sample in the sample injection system to the cell imaging system.

[0072] The cell imaging system is arranged in the optical detection system, and the output end of the optical detection system is connected to the input end of the data processing and analysis system.

[0073] The optical detection system is used to perform high-definition processing on the cell image output by the cell imaging system to obtain a high-definition image and transmit the high-definition image to the data processing and analysis system.

[0074] The data processing and analysis system is used to perform cell detection on the high-definition image.

[0075] Furthermore, the cell detection system further includes: a second driving device and a sample output system.

[0076] The output end of the cell imaging system is connected to the input end of the sample output system. The second driving device is connected between the cell imaging system and the sample injection system for transporting the cell sample that has completed cell imaging in the cell imaging system to the sample output system.

[0077] The first driving device and the second driving device can be various driving devices such as peristaltic pumps or pressure pumps, which are not limited herein.

[0078] In the system of this embodiment, when the cell sample flows through the slit layer, the cells are captured and imaged by the optical lens of the imaging device. After high-definition processing by the optical detection system, the high-definition cell image is transmitted to the data processing and analysis system for analysis, realizing detection processes such as cell recognition, segmentation, classification, counting, and super-resolution reconstruction. At the same time, the cell sample that has completed imaging will be transported to the sample output system for recycling processing, forming a cycle of cell imaging and detection. Through the cooperation of the entire system, real-time continuous imaging of large-throughput, large-volume, and high-concentration cell samples can be achieved, and further real-time continuous detection of such cell samples can be realized, improving the stability and accuracy of detection.

[0079] Figure 6 It is a schematic flowchart of the cell detection method provided by the embodiment of the present application; referring to Figure 6 , the embodiment of the present application provides a cell detection method, which may include:

[0080] 601. Control the first driving device to transport the cell sample in the sample injection system to the cell imaging system.

[0081] 602. Control the cell imaging system to image the cell sample to obtain a cell image.

[0082] 603. Control the optical detection system to perform high-definition processing on the cell image to obtain a high-definition image.

[0083] 604. Control the optical detection system to transmit high-definition images to the data processing and analysis system;

[0084] 605. Control the data processing and analysis system to perform cell detection on the high-definition images.

[0085] Before step 601, a certain volume of cell samples can be injected into the sampling system first, and then the pinch valve at the output end of the sampling system is closed to set up the cell detection system: fix the cell imaging system in the optical detection system, seamlessly connect the pipelines at both ends of the cell imaging chip to the sampling system and the sample output system, connect the first driving device between the sampling system and the cell imaging chip system, connect the second driving device between the sample output system and the cell imaging chip system, and finally complete the connection between the optical detection system and the backend data processing and analysis system to ensure continuous real-time measurement of cell samples.

[0086] After the cell detection system is set up, turn on the power supply and adjust the performance parameters of the imaging device according to the working environment, including the focusing of the imaging device, the optical imaging magnification, etc. This magnification can be set according to actual needs and is not limited here. In this embodiment, the magnification can be set to 20 times. In addition, the data output parameters of the data processing and analysis system can also be adjusted, mainly including the acquisition accuracy, acquisition time, acquisition quantity, etc., and set the sampling parameters of the sampling system, mainly including the sampling speed and sampling time, etc.

[0087] In step 601, open the sampling system and wait for the cell samples to fill the gap layer;

[0088] In step 602, at this time, close the sampling system. After the cell samples in the gap layer are stable, turn on the imaging device and control it to image the cell samples in the gap layer in the observation window to obtain cell images;

[0089] In step 603, since the cell samples are not in a static state after entering the gap layer, certain liquid fluctuations will cause deviations in imaging clarity. Therefore, it is necessary to control the optical detection system to remove abnormal imaging results in the cell images, and finally obtain a microscopic imaging result of cell samples with high clarity; at the same time, open the sampling system again to inject cell samples into the gap layer, and the cell samples that have completed imaging will flow out of the gap layer to the sample output system to start a new round of cell imaging to ensure uninterrupted cell imaging;

[0090] In step 605, the cell detection method in the backend data processing and analysis system can be selected according to the actual situation, such as the frame difference method, background difference method, etc., which is not limited here.

[0091] In the method of this embodiment, when the cell sample flows through the slit layer, the cells are captured and imaged by the optical lens of the imaging device. After being processed in high definition by the optical detection system, the high-definition cell images are transmitted to the data processing and analysis system for analysis, realizing detection processes such as cell recognition, segmentation, classification, counting, and super-resolution reconstruction. At the same time, the cell samples that have completed imaging are conveyed to the sample output system for recycling, forming a cycle of cell imaging and detection, thereby realizing real-time continuous imaging of large-throughput, large-volume, and high-concentration cell samples, and further realizing real-time continuous detection of such cell samples, improving the stability and accuracy of detection.

[0092] Figure 7 An example of the structural schematic diagram of an electronic device is shown as Figure 7 shown. The electronic device may include: a processor 710, a communication interface 720, a memory 730, and a communication bus 740. Among them, the processor 710, the communication interface 720, and the memory 730 complete communication with each other through the communication bus 740. The processor 710 can call the computer program in the memory 730 to execute the steps of the cell detection method, for example, including:

[0093] Controlling the first driving device to convey the cell sample in the sample input system to the cell imaging system;

[0094] Controlling the cell imaging system to image the cell sample to obtain cell images;

[0095] Controlling the optical detection system to perform high-definition processing on the cell images to obtain high-definition images;

[0096] Controlling the optical detection system to transmit the high-definition images to the data processing and analysis system;

[0097] Controlling the data processing and analysis system to perform cell detection on the high-definition images.

[0098] In addition, when the logical instructions in the above-mentioned memory 730 are implemented in the form of software functional units and sold or used as independent products, they can be stored in a computer-readable storage medium. Based on such an understanding, the technical solution of this application, in essence, or the part that contributes to the prior art, or a part of this technical solution, can be embodied in the form of a software product. This computer software product is stored in a storage medium and includes several instructions for causing a computer device (which can be a personal computer, a server, or a network device, etc.) to execute all or part of the steps of the methods described in various embodiments of this application. The aforementioned storage medium includes: various media such as USB flash drives, mobile hard disks, read-only memories (ROM, Read-Only Memory), random access memories (RAM, Random Access Memory), magnetic disks, or optical discs that can store program codes.

[0099] On the other hand, an embodiment of this application also provides a computer program product. The computer program product includes a computer program. The computer program can be stored on a non-transitory computer-readable storage medium. When the computer program is executed by a processor, the computer can execute the steps of the cell detection method provided in the above-mentioned various embodiments. For example, it includes:

[0100] Controlling a first driving device to transport the cell sample in the sampling system to the cell imaging system;

[0101] Controlling the cell imaging system to image the cell sample to obtain a cell image;

[0102] Controlling an optical detection system to perform high-definition processing on the cell image to obtain a high-definition image;

[0103] Controlling the optical detection system to transmit the high-definition image to the data processing and analysis system;

[0104] Controlling the data processing and analysis system to perform cell detection on the high-definition image.

[0105] On the other hand, an embodiment of this application also provides a processor-readable storage medium. The processor-readable storage medium stores a computer program. The computer program is used to cause a processor to execute the steps of the methods provided in the above-mentioned various embodiments. For example, it includes:

[0106] Controlling a first driving device to transport the cell sample in the sampling system to the cell imaging system;

[0107] Controlling the cell imaging system to image the cell sample to obtain a cell image;

[0108] Controlling an optical detection system to perform high-definition processing on the cell image to obtain a high-definition image;

[0109] Control the optical detection system to transmit the high-definition image to the data processing and analysis system;

[0110] Control the data processing and analysis system to perform cell detection on the high-definition image.

[0111] The processor-readable storage medium can be any available medium or data storage device accessible by the processor, including but not limited to magnetic memory (such as floppy disks, hard disks, magnetic tapes, magneto-optical discs (MO), etc.), optical memory (such as CDs, DVDs, BDs, HVDs, etc.), and semiconductor memory (such as ROM, EPROM, EEPROM, non-volatile memory (NANDFLASH), solid-state drives (SSD)), etc.

[0112] Through the description of the above embodiments, those skilled in the art can clearly understand that each embodiment can be implemented by means of software plus a necessary general hardware platform, and of course, it can also be implemented by hardware. Based on such an understanding, the above technical solution, in essence, or the part that contributes to the prior art, can be embodied in the form of a software product. This computer software product can be stored in a computer-readable storage medium, such as ROM / RAM, magnetic disks, optical discs, etc., and includes several instructions for causing a computer device (which can be a personal computer, a server, or a network device, etc.) to execute the methods described in each embodiment or some parts of the embodiments.

[0113] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present application, and are not intended to limit them; although the present application has been described in detail with reference to the foregoing embodiments, those of ordinary skill in the art should understand that they can still modify the technical solutions described in the foregoing embodiments, or perform equivalent replacements on some of the technical features; and these modifications or replacements do not make the essence of the corresponding technical solutions deviate from the spirit and scope of the technical solutions of each embodiment of the present application.

Claims

1. A cell imaging chip, characterized in that, Comprising: A laminate and an imaging plate; The imaging plate is disposed at the bottom of the laminate, and a gap layer is provided between the imaging plate and the laminate; A diversion channel is provided on the laminate. The diversion channel includes a liquid inlet channel and at least one diversion groove. The diversion grooves are respectively communicated with the liquid inlet channel and the gap layer, so as to divert the cell sample flowing into the diversion groove from the liquid inlet channel to the gap layer and then perform cell imaging.

2. The cell imaging chip according to claim 1, wherein: The diversion channel further includes a liquid outlet channel, and the liquid outlet channel is disposed on the opposite side of the liquid inlet channel; The diversion grooves are uniformly distributed on both sides of the liquid outlet channel and the liquid inlet channel, and the diversion groove includes a first extension groove and a second extension groove; The first extension groove extends towards the center of the chip, and the first extension groove is isolated from the gap layer. The second extension groove has a different extension direction from the first extension groove, and the second extension groove is communicated with the gap layer.

3. The cell imaging chip according to claim 2, wherein: The first diversion groove on the side of the liquid inlet channel is disposed at the first bottom of the liquid inlet channel away from the liquid inlet, and one end of the first extension groove of the first diversion groove is communicated with the first bottom, and the other end of the first extension groove of the first diversion groove is communicated with the second extension groove of the first diversion groove.

4. The cell imaging chip according to claim 2, wherein: The second diversion groove on the side of the liquid outlet channel is disposed at the second bottom of the liquid outlet channel away from the liquid outlet, and one end of the first extension groove of the second diversion groove is communicated with the second bottom, and the other end of the first extension groove of the second diversion groove is communicated with the second extension groove of the second diversion groove.

5. The cell imaging chip according to claim 1, wherein: A groove is provided at the center position of the top of the laminate, and an observation window is provided at the bottom of the groove for observing the cell sample in the gap layer and providing a shooting position for cell imaging.

6. A cell imaging system, characterized in that, Comprising: An imaging device and the cell imaging chip according to any one of claims 1 to 5; The imaging device is disposed on the perpendicular line of the plane where the gap layer of the cell imaging chip is located, and is used for photographing the cell sample in the gap layer.

7. A cell detection system, characterized in that, Comprising: A sample injection system, a first driving device, an optical detection system, a data processing and analysis system, and the cell imaging system according to claim 6; The output end of the sample injection system is connected to the output end of the cell imaging system, and the first driving device is connected between the sample injection system and the cell imaging system for transporting the cell sample in the sample injection system to the cell imaging system; The cell imaging system is disposed in the optical detection system, and the output end of the optical detection system is connected to the input end of the data processing and analysis system; The optical detection system is used for performing high-definition processing on the cell image output by the cell imaging system to obtain a high-definition image, and transmitting the high-definition image to the data processing and analysis system; The data processing and analysis system is used for performing cell detection on the high-definition image.

8. The cell detection system according to claim 7, wherein It further includes: Second driving device and sample output system; The output end of the cell imaging system is connected to the input end of the sample output system, and the second driving device is connected between the cell imaging system and the sample output system for transporting the cell sample that has completed cell imaging in the cell imaging system to the sample output system.

9. A cell detection method, characterized in that, Implemented by using the cell detection system according to claim 7 or 8, including: Controlling the first driving device to transport the cell sample in the sample injection system to the cell imaging system; Controlling the cell imaging system to image the cell sample to obtain a cell image; Controlling the optical detection system to perform high-definition processing on the cell image to obtain a high-definition image; Controlling the optical detection system to transmit the high-definition image to the data processing and analysis system; Controlling the data processing and analysis system to perform cell detection on the high-definition image.

10. The cell detection method according to claim 9, wherein The controlling the cell imaging system to image the cell sample to obtain a cell image includes: Controlling the imaging device in the cell imaging system to image the cell sample in the slit layer at the observation window to obtain a cell image.

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