Portable fluorescence detection equipment for detecting berberine and application

Abstract

The invention discloses portable fluorescence detection equipment for detecting berberine and application. The portable fluorescence detection equipment comprises a shell, and a sample supporting module, an excitation module, a photoelectric conversion module, a voltage detection module, a power supply module and a sample pool which are arranged in the shell, the sample supporting module is a cylinder with two hollow supporting arms which are perpendicular to each other; the excitation module is composed of an excitation light source and a first optical filter, and the excitation module is installed on the hollow support arm on one side of the sample support module; the photoelectric conversion module is composed of a photoelectric converter and a second optical filter, and the photoelectric conversion module is installed on the hollow supporting arm on the other side of the sample supporting module. The invention provides portable fluorescence detection equipment for detecting berberine, which is simple in structure and reasonable in design, so that real-time or in-situ fluorescence detection of berberine in an actual sample can be realized; and the device has the advantages of small size and convenience in carrying, and is convenient for actual use.

Description

Technical Field

[0001] The present invention belongs to the technical field of berberine fluorescence detection equipment and detection methods, and particularly relates to a portable fluorescence detection equipment for detecting berberine (also known as berberine) and its application. Background Art

[0002] Fluorescence detection is a commonly used detection technique, which qualitatively and quantitatively analyzes an analyte by utilizing the property that the analyte will generate fluorescence when excited by light of a specific wavelength. This method is not only convenient and fast, but also usually has high sensitivity and selectivity, so it is very easy to be used for real-time or in-situ detection and analysis. However, at present, generally existing fluorescence detection devices are relatively large in volume, complex in structure, require a relatively large number of electronic components, and are relatively high in price. It is difficult to achieve real-time or in-situ detection, and it is not easy to popularize in economically underdeveloped areas. Summary of the Invention

[0003] The technical problem solved by the present invention is to provide a portable fluorescence detection equipment for detecting berberine, and this portable fluorescence detection equipment can be used for rapid quantitative analysis of berberine in actual samples.

[0004] The present invention adopts the following technical solutions to solve the above technical problems. A portable fluorescence detection equipment for detecting berberine, which is characterized by comprising a housing and a sample support module, an excitation module, a photoelectric conversion module, a voltage detection module, a power supply module and a sample cell arranged inside the housing; the sample support module is a cylinder with two hollow arms arranged perpendicular to each other, the cylinder in the sample support module is internally connected with the hollow arms, and the hollow arms are all perpendicular to the cylinder. The sample cell is arranged inside the cylinder for holding samples; the excitation module is composed of an excitation light source and a first filter, and this excitation module is installed on the hollow arm on one side of the sample support module, wherein the excitation light direction of the excitation light source is opposite to the first filter, and the pure excitation light directly irradiates the sample in the sample cell after passing through the first filter; the photoelectric conversion module is composed of a photoelectric converter and a second filter, and this photoelectric conversion module is installed on the hollow arm on the other side of the sample support module, wherein the emitted light emitted by the sample after being excited by the excitation light is opposite to the second filter, and the pure emitted light directly irradiates the photoelectric converter after passing through the second filter; the voltage detection module is composed of a voltage detection circuit and a voltage display, wherein the voltage detection circuit is respectively connected to the photoelectric converter and the voltage display through wires; the power supply module is composed of a battery and a voltage stabilizing circuit for supplying power to the equipment.

[0005] Further defined, the portable fluorescence detection device further includes a Bluetooth module, which is a Bluetooth transmitting device installed on the voltage detection module, mainly used for communicating the portable fluorescence detection device with a mobile phone, displaying or storing the measured data on the mobile phone, making the measured data easier to transmit and store.

[0006] Further defined, the portable fluorescence detection device further includes a heat dissipation module, which consists of a heat sink, a cooling fan and a heat dissipation window, and is used to dissipate excess heat for the detection device; a voltage display, a range adjustment button, a Bluetooth switch button and a display adjustment switch are provided on the upper control panel of the housing, and a charging interface, a power switch and an external power interface are provided on the side plate of the housing; a light shield is provided on the upper part of the cylinder.

[0007] The application of the portable fluorescence detection device of the present invention in detecting berberine is characterized in that the specific process is as follows:

[0008] Step S1, disperse the TPDCA fluorescence probe in deionized water to obtain a TPDCA fluorescence probe solution with a concentration of 7.5 μg / mL. Take 0.5 mL of the TPDCA fluorescence probe solution with a concentration of 7.5 μg / mL and add different volumes of berberine standard solution with a concentration of 0.5 mg / mL, and then make up the volume to 5 mL with deionized water to prepare berberine standard solutions with concentrations of 0 μg / mL, 0.1 μg / mL, 0.5 μg / mL, 1 μg / mL, 5 μg / mL, 10 μg / mL, 20 μg / mL, 40 μg / mL, 60 μg / mL, 80 μg / mL, 100 μg / mL, 120 μg / mL, 140 μg / mL, 160 μg / mL, 180 μg / mL and 200 μg / mL. After mixing evenly, react at 25 °C for 2.0 min, and use the portable fluorescence detection device to measure the voltage of the mixed system before and after adding berberine standard solutions with different concentrations at an excitation wavelength of 348 nm and an emission wavelength of 428 nm. When the berberine concentration is in the range of 0.1 - 80 μg / mL, there is a good linear relationship between the fluorescence quenching efficiency V / V0, and the linear equation is V / V0 = -7.51×10 -3 X + 0.99807, the correlation coefficient R 2 = 0.9997, where V0 and V are the voltages of the mixed system before and after adding the berberine standard solution respectively, and X is the concentration of the berberine standard solution, with the unit μg / mL;

[0009] Step S2: Take 0.5 mL of the TPDCA fluorescent probe solution with a concentration of 7.5 μg / mL and add it to the sample solution containing berberine to be measured. Then, make up the volume to 5 mL with deionized water. After mixing evenly, react for 2.0 min at 25°C. Use a portable fluorescence detection device to measure the voltage of the mixed system before and after adding the sample solution containing berberine to be measured at an excitation wavelength of 348 nm and an emission wavelength of 428 nm. Then, based on the measured voltage of the mixed system before and after adding the sample solution containing berberine to be measured, and combined with the linear equation obtained above, calculate the concentration of berberine in the sample solution containing berberine to be measured, with the unit μg / mL.

[0010] Compared with the prior art, the present invention has the following advantages and beneficial effects: 1. The present invention provides a portable fluorescence detection device for detecting berberine with a simple structure and reasonable design, which can realize real-time or in-situ fluorescence detection of berberine in actual samples; and has the advantages of small size and easy portability, which is convenient for actual use; 2. The portable fluorescence detection device based on the TPDCA fluorescent probe of the present invention can realize the detection of berberine in different samples, has good sensitivity and high accuracy. There is a good linear relationship between the concentration of berberine in the range of 0.1 - 80 μg / mL and the fluorescence quenching efficiency V / V0 obtained by the portable fluorescence detection device, and it can be used for rapid quantitative analysis of berberine in actual samples. Description of the Drawings

[0011] Figure 1 It is a schematic diagram of the external structure of the portable fluorescence detection device.

[0012] Figure 2 It is a schematic diagram of the internal structure of the portable fluorescence detection device.

[0013] Figure 3 It is a schematic diagram of the working principle of the portable fluorescence detection device.

[0014] In the figure: 1 - housing, 2 - hollow arm, 3 - cylinder, 4 - excitation light source, 5 - first filter, 6 - photoelectric converter, 7 - second filter, 8 - light shield, 9 - voltage display, 10 - range adjustment button, 11 - Bluetooth switch button, 12 - display adjustment switch, 13 - charging interface, 14 - power switch, 15 - external power interface.

[0015] Figure 4 It is the 1H NMR spectrum of the TPDCA fluorescent probe.

[0016] Figure 5 It is the mass spectrum of the TPDCA fluorescent probe.

[0017] Figure 6It is the fluorescence performance graph of the TPDCA fluorescent probe. Inset: Pictures of TPDCA under natural light (left) and 365 nm ultraviolet light (right).

[0018] Figure 7 It is the quantum yield graph of the TPDCA fluorescent probe.

[0019] Figure 8 It is the energy level distribution graph of the HOMO orbital (left) and LUMO orbital (right) of the TPDCA fluorescent probe.

[0020] Figure 9 It is the quenching effect of berberine on the TPDCA fluorescent probe. Inset: Pictures of TPDCA (left) and TPDCA + berberine (right) under ultraviolet light.

[0021] Figure 10 It is the response curve of the portable fluorescence detection device based on the TPDCA fluorescent probe to berberine.

[0022] Figure 11 It is the linear equation of the portable fluorescence detection device based on the TPDCA fluorescent probe for detecting berberine.

[0023] Figure 12 It is the linear equation of the high performance liquid chromatography (HPLC) based on the TPDCA fluorescent probe for detecting berberine.

[0024] Figure 13 It is the selectivity of the high performance liquid chromatography (HPLC) for the determination of berberine content. Detailed implementation manners

[0025] The above content of the present invention will be further described in detail through the following embodiments. However, it should not be understood that the scope of the above subject matter of the present invention is limited to the following embodiments. Any technology implemented based on the above content of the present invention belongs to the scope of the present invention.

[0026] Such as Figures 1-3As shown in the figure, the portable fluorescence detection device designed by the present invention for detecting berberine includes a housing 1 and a sample support module, an excitation module, a photoelectric conversion module, a voltage detection module, a power supply module and a sample cell arranged inside the housing 1; the sample support module is a cylinder 3 with two hollow arms 2 arranged perpendicular to each other, the cylinder 3 in the sample support module is internally connected to the hollow arms 2, and the hollow arms 2 are all perpendicular to the cylinder 3, and the sample cell is arranged inside the cylinder 3 for holding samples; the excitation module is composed of an excitation light source 4 and a first filter 5, and this excitation module is installed on the hollow arm 2 on one side of the sample support module, wherein the excitation light direction of the excitation light source 4 is opposite to the first filter 5, and the pure excitation light directly irradiates the sample in the sample cell after passing through the first filter 5; the photoelectric conversion module is composed of a photoelectric converter 6 and a second filter 7, and this photoelectric conversion module is installed on the hollow arm 2 on the other side of the sample support module, wherein the emitted light emitted by the sample after being excited by the excitation light is opposite to the second filter 7, and the pure emitted light directly irradiates the photoelectric converter 6 after passing through the second filter 7; the voltage detection module is composed of a voltage detection circuit and a voltage display 9, wherein the voltage detection circuit is respectively connected to the photoelectric converter and the voltage display 9 through wires; the power supply module is composed of a battery and a voltage stabilizing circuit for supplying power to the device.

[0027] The portable fluorescence detection device of the present invention further includes a Bluetooth module, and this Bluetooth module is a Bluetooth transmitting device installed on the voltage detection module, mainly used for communicating the portable fluorescence detection device with a mobile phone, displaying or storing the measured data in the mobile phone, making the measured data easier to transmit and store, and further avoiding mistakes caused by manual recording.

[0028] The portable fluorescence detection device of the present invention further includes a heat dissipation module, and this heat dissipation module is composed of a heat sink, a cooling fan and a heat dissipation window for dissipating excess heat from the detection device; on the upper control panel of the housing 1, there are a voltage display 9, a range adjustment button 10, a Bluetooth switch button 11 and a display adjustment switch 12, and on the side plate of the housing 1, there are a charging interface 13, a power switch 14 and an external power interface 15; on the upper part of the cylinder 3, there is a light shield 8.

[0029] The bottom wall of the sample cell of the present invention is a transparent bottom wall and is equipped with a sample cap, and this sample cell is closely attached to the sample support. Such a scheme can avoid stray light and further improve the measurement accuracy. A voltage stabilizing circuit is installed in the power supply module, which can effectively avoid the change of the optical signal of the excitation light source caused by the battery voltage fluctuation, and further improve the measurement accuracy. The sample cell is in a cylindrical shape, which can make the sample cell more convenient to insert into the sample support for convenient detection. The side wall and the bottom wall of the sample cell are both made of quartz glass. Such a scheme makes the excited fluorescence not easily absorbed by the sample and avoids affecting the detection.

[0030] The portable fluorescence detection device described in the present invention is equipped with an external power supply interface and can be powered by an external 5V power supply (for example, a power bank), which improves the battery life of the portable device. Such a structure can effectively improve the performance of real-time or in-situ detection of the portable detection device.

[0031] With the help of photoelectric conversion technology, rapid and portable detection of fluorescence intensity can be realized. In order to achieve the function of real-time or in-situ detection and analysis of the portable fluorescence detection device, a detection method using photoelectric conversion is adopted. The excitation light is filtered by a filter to remove unnecessary excitation light and then irradiates the sample. The fluorescent substance in the sample is excited to generate fluorescence. The filter removes non-sample fluorescence from the fluorescence, and then the photoelectric conversion device converts the fluorescence signal of the sample into an electrical signal. The intensity of the electrical signal is used to judge the intensity of the sample fluorescence, so as to realize the rapid quantitative analysis of the detected substance in the sample.

[0032] The chemical name of the fluorescent probe used in the detection process of the present invention is: 5-oxo-2,3-dihydro-5H-thiazolo[3,2-a]pyridine-3,7-dicarboxylic acid, abbreviation: TPDCA, and its structure is shown below. The structural analysis of this fluorescent probe is as follows: From the single crystal structure of 5-oxo-2,3-dihydro-5H-thiazolo[3,2-a]pyridine-3,7-dicarboxylic acid (TPDCA), it can be seen that the structure of TPDCA contains nitrogen and sulfur atoms and two carboxyl groups.

[0033]

[0034] Figure 4 This is the 1H NMR spectrum of the TPDCA fluorescent probe: From the 1H NMR data of TPDCA, it can be seen that it is consistent with the molecular formula C9H7NO5S. Figure 5 This is the mass spectrum of the TPDCA fluorescent probe: From the HRMS (ESI-TOF) m / z of TPDCA, it can be seen that the molecular formula of the TPDCA fluorescent probe is C9H7NO5S, and the proton peak is 239.9962, which is consistent with the calculated value.

[0035] Figure 6 This is the fluorescence performance diagram of the TPDCA fluorescent probe. Insert: Pictures of TPDCA under natural light (left) and 365 nm ultraviolet light (right). From Figure 6 the insert, it can be seen that TPDCA has strong fluorescence. The excitation peak and emission peak of the TPDCA fluorescent probe are located at 348 nm and 428 nm respectively. Figure 7is the quantum yield of the TPDCA fluorescent probe. The quanta emitted by TPDCA in the range of 363 nm to 700 nm were collected using an Edinburgh (UK) FLS980 fluorometer equipped with an integrating sphere. Using the solvent (water) as the blank, the quantum yield of TPDCA was directly calculated using the software provided by the fluorometer. The quantum yield of TPDCA is as high as 80.65%. Figure 8 is the energy level distribution diagram of the HOMO orbital (left) and LUMO orbital (right) of the TPDCA fluorescent probe. From the orbital energy level distribution diagram of the TPDCA fluorescent probe, it can be seen that the energy levels of the highest occupied molecular orbital (HOMO) and the lowest unoccupied molecular orbital (LUMO) of the TPDCA fluorescent probe are mainly distributed on the conjugated system of the six-membered ring in the TPDCA molecule. The HOMO and LUMO energy levels of the TPDCA fluorescent probe were calculated using quantum chemical calculation methods (DFT / B3LYP / 6-31+G(d)), and the results were that the HOMO energy level was -6.283 eV, the LUMO energy level was -2.368 eV, and the band gap was 3.915 eV.

[0036] Figure 9 is the quenching effect of berberine on the TPDCA fluorescent probe. Inset: Pictures of TPDCA (left) and TPDCA + berberine (right) under ultraviolet light. From Figure 9 the inset, it can be seen that the fluorescence of the TPDCA fluorescent probe has basically disappeared after adding berberine. From Figure 9 it can be seen that after adding berberine, the fluorescence intensity of berberine has decreased significantly. Therefore, the TPDCA fluorescent probe can be used for the fluorescence detection of berberine.

[0037] Figure 10 is the response curve of the portable fluorescence detection device based on the TPDCA fluorescent probe to berberine. From Figure 10 it can be seen that as the concentration of berberine increases, the voltage of the portable fluorescence detection device gradually decreases, and in the range of berberine concentration from 0.1 to 80 μg / mL, the voltage detected by the portable fluorescence detection device is linearly correlated with the concentration of berberine.

[0038] Figure 11 is the linear equation of berberine detected by the portable fluorescence detection device based on the TPDCA fluorescent probe. The linear equation between the quenching efficiency of the TPDCA fluorescent probe and the concentration of berberine is V / V0 = -7.51×10 -3 X + 0.99807, and the correlation coefficient R 2 = 0.9997, where V0 and V are the fluorescence intensities of the mixed system before and after adding the berberine standard solution respectively, and X is the concentration of the berberine standard solution, with the unit μg / mL. The berberine standard solution with a concentration of 40 μg / mL was measured 11 times repeatedly, and the detection limit was calculated to be 28.32 ng / mL according to the relative standard deviation.

[0039] Linear equation for the detection of berberine by a portable fluorescence detection device based on the TPDCA fluorescence probe:

[0040] Disperse the TPDCA fluorescence probe in deionized water to obtain a TPDCA fluorescence probe solution with a concentration of 7.5 μg / mL. Take 0.5 mL of the TPDCA fluorescence probe solution with a concentration of 7.5 μg / mL and add different volumes of a berberine standard solution with a concentration of 0.5 mg / mL, and then make up the volume to 5 mL with deionized water to prepare berberine standard solutions with concentrations of 0 μg / mL, 0.1 μg / mL, 0.5 μg / mL, 1 μg / mL, 5 μg / mL, 10 μg / mL, 20 μg / mL, 40 μg / mL, 60 μg / mL, 80 μg / mL, 100 μg / mL, 120 μg / mL, 140 μg / mL, 160 μg / mL, 180 μg / mL, and 200 μg / mL. After mixing evenly, react at 25 °C for 2.0 min. Use a portable fluorescence detection device to measure the voltage of the mixed system before and after adding berberine standard solutions with different concentrations at an excitation wavelength of 348 nm and an emission wavelength of 428 nm. When the berberine concentration is in the range of 0.1 - 80 μg / mL, there is a good linear relationship between the berberine concentration and the fluorescence quenching efficiency V / V0. The linear equation is V / V0 = -7.51×10 -3 X + 0.99807, correlation coefficient R 2 = 0.9997, where V0 and V are the voltages of the mixed system before and after adding the berberine standard solution respectively, and X is the concentration of the berberine standard solution, with the unit μg / mL;

[0041] The detection process for the sample solution containing berberine to be measured is as follows:

[0042] Take 0.5 mL of the TPDCA fluorescence probe solution with a concentration of 7.5 μg / mL and add the sample solution containing berberine to be measured, and then make up the volume to 5 mL with deionized water. After mixing evenly, react at 25 °C for 2.0 min. Use a portable fluorescence detection device to measure the voltage of the mixed system before and after adding the sample solution containing berberine to be measured at an excitation wavelength of 348 nm and an emission wavelength of 428 nm. Then, based on the measured voltage of the mixed system before and after adding the sample solution containing berberine to be measured, and combined with the above-obtained linear equation, calculate the berberine concentration in the sample solution containing berberine to be measured, with the unit μg / mL.

[0043] Figure 12 This is the linear equation for the determination of the content of berberine by high performance liquid chromatography (HPLC). In the range of 0.5 - 300 μg / mL, the linear equation between the berberine concentration and the peak area is y = -7.51×10 -3 x + 0.99807, correlation coefficient R2 = 0.9995, where y is the added peak area and x is the concentration of the berberine standard solution in μg / mL. Figure 13 It is the selectivity for the determination of berberine content by high performance liquid chromatography (HPLC). The retention time of berberine is 5.9 min, and the resolution is good.

[0044] Example 1

[0045] Determination method and application of a portable fluorescence detection device in the determination of berberine in actual samples

[0046] Samples of Cortex Phellodendri Chinensis slices, Rhizoma Coptidis slices, Coptis capsules and Compound Berberine Tablets were collected. The samples were extracted, filtered, centrifuged and concentrated (or diluted) to prepare a solution with a certain concentration.

[0047] The extraction and filtration process is as follows:

[0048] Cortex Phellodendri Chinensis slices, purchased from Chuanrentang Pharmacy in Muye District, Xinxiang City, were ground into fine powder. Take 50.0 mg of Cortex Phellodendri Chinensis fine powder, add 50 mL of deionized water and extract by ultrasound for 30 min. The extract was centrifuged at 12000 r / min for 20 min, and the supernatant was filtered through a 0.22 μm microporous membrane to obtain the solution.

[0049] Rhizoma Coptidis slices, purchased from Chuanrentang Pharmacy in Muye District, Xinxiang City, were ground into fine powder. Take 29.0 mg of Rhizoma Coptidis fine powder, add 50 mL of deionized water and extract by ultrasound for 30 min. The extract was centrifuged at 12000 r / min for 20 min, and the supernatant was filtered through a 0.22 μm microporous membrane to obtain the solution.

[0050] Coptis capsules, purchased from Wenzhou Zhengda Pharmacy Chain Co., Ltd., had their capsule shells removed. Take 30.6 mg of the contents of Coptis capsules, add 50 mL of deionized water and extract by ultrasound for 30 min. The extract was centrifuged at 12000 r / min for 20 min, and the supernatant was filtered through a 0.22 μm microporous membrane to obtain the solution.

[0051] Compound Berberine Tablets, purchased from Wenzhou Zhengda Pharmacy Chain Co., Ltd., had their sugar coating removed and were placed in a mortar and ground. Take 18.6 mg of the contents of Coptis capsules, add 50 mL of deionized water and extract by ultrasound for 30 min. The extract was centrifuged at 12000 r / min for 20 min, and the supernatant was filtered through a 0.22 μm microporous membrane to obtain the solution.

[0052] After adding a certain amount of berberine and mixing evenly, the portable fluorescence detection device based on the TPDCA fluorescence probe constructed by the present invention verified the accuracy of the portable device by the method of standard addition recovery. The results of the standard addition method are shown in Table 1. The recovery rate of added berberine was between 97.10% and 100.18%, and the relative standard deviation (RSD) was 0.06% to 0.39%. This indicates that the portable fluorescence detection device based on the TPDCA fluorescence probe constructed by the present invention is feasible and reliable for the determination of berberine in actual drug samples.

[0053] Table 1 Recovery rate of the portable fluorescence detection device for detecting berberine in actual samples (n = 3)

[0054]

[0055] The collected samples of Cortex Phellodendri Chinensis, Rhizoma Coptidis, Coptis capsules, and compound berberine tablets were extracted, filtered, centrifuged, and concentrated (or diluted) to prepare solutions with a certain concentration. The content was determined using the portable fluorescence detection device based on the TPDCA fluorescence probe constructed by the present invention and high performance liquid chromatography. The detection results of the portable fluorescence detection device and high performance liquid chromatography are shown in Table 2. The relative standard deviation (RSD) of the detection results of the portable fluorescence detection device based on the TPDCA fluorescence probe constructed by the present invention was 0.32% to 0.54%. Through one-way analysis of variance, it was found that there was no significant difference in the detection results compared with those of high performance liquid chromatography (P > 0.05). This indicates that the portable fluorescence detection device based on the TPDCA fluorescence probe constructed by the present invention is feasible and reliable for the determination of berberine in actual drug samples.

[0056] Table 2 Comparison of the detection results of different samples by the portable fluorescence detection device and high performance liquid chromatography (n = 3)

[0057]

[0058]

[0059] The above embodiments describe the basic principles, main features, and advantages of the present invention. Those skilled in the art should understand that the present invention is not limited by the above embodiments. What is described in the above embodiments and the specification only illustrates the principles of the present invention. Without departing from the scope of the principles of the present invention, the present invention will have various changes and improvements, and these changes and improvements all fall within the scope of protection of the present invention.

Claims

1. A portable fluorescence detection device for detecting berberine, characterized in that It includes a housing and a sample support module, an excitation module, a photoelectric conversion module, a voltage detection module, a power supply module and a sample cell disposed inside the housing; the sample support module is a cylinder with two hollow arms arranged perpendicular to each other, the cylinder in the sample support module is internally connected to the inside of the hollow arms and the hollow arms are all perpendicular to the cylinder, and the sample cell is disposed inside the cylinder for holding the sample; the excitation module consists of an excitation light source and a first filter, and this excitation module is installed on the hollow arm on one side of the sample support module, wherein the excitation light direction of the excitation light source is opposite to the first filter and the pure excitation light directly irradiates the sample in the sample cell after passing through the first filter; the photoelectric conversion module consists of a photoelectric converter and a second filter, and this photoelectric conversion module is installed on the hollow arm on the other side of the sample support module, wherein the emitted light emitted after the sample is excited is opposite to the second filter and the pure emitted light directly irradiates the photoelectric converter after passing through the second filter; the voltage detection module consists of a voltage detection circuit and a voltage display, wherein the voltage detection circuit is respectively connected to the photoelectric converter and the voltage display through lines; the power supply module is composed of a battery and a voltage stabilizing circuit for supplying power to the device.

2. The portable fluorescence detection device for detecting berberine according to claim 1, wherein The portable fluorescence detection device further includes a Bluetooth module, and this Bluetooth module is a Bluetooth transmitting device installed on the voltage detection module, mainly used for communicating the portable fluorescence detection device with a mobile phone, displaying or storing the measured data on the mobile phone, making the measured data easier to transmit and store.

3. The portable fluorescence detection device for detecting berberine according to claim 1, characterized in that The portable fluorescence detection device further includes a heat dissipation module, and this heat dissipation module consists of a heat sink, a cooling fan and a heat dissipation window for dissipating excess heat from the detection device; on the upper control panel of the housing, there are a voltage display, a range adjustment button, a Bluetooth switch button and a display adjustment switch, and on the side plate of the housing, there are a charging interface, a power switch and an external power interface; a light shield is provided on the upper part of the cylinder.

4. Use of the portable fluorescence detection device according to any one of claims 1 to 3 in the detection of berberine, characterized in that The specific process is as follows: Step S1: Disperse 5-oxo-2,3-dihydro-5H-thiazolo[3,2-a]pyridine-3,7-dicarboxylic acid (TPDCA) fluorescent probe in deionized water to obtain a TPDCA fluorescent probe solution with a concentration of 7.5 μg / mL. Take 0.5 mL of the TPDCA fluorescent probe solution with a concentration of 7.5 μg / mL and add different volumes of berberine standard solution with a concentration of 0.5 mg / mL, and then make up the volume to 5 mL with deionized water to prepare berberine standard solutions with concentrations of 0 μg / mL, 0.1 μg / mL, 0.5 μg / mL, 1 μg / mL, 5 μg / mL, 10 μg / mL, 20 μg / mL, 40 μg / mL, 60 μg / mL, 80 μg / mL, 100 μg / mL, 120 μg / mL, 140 μg / mL, 160 μg / mL, 180 μg / mL, and 200 μg / mL. After mixing evenly, react at 25 °C for 2.0 min, and use a portable fluorescence detection device to measure the voltage of the mixed system before and after adding berberine standard solutions with different concentrations at an excitation wavelength of 348 nm and an emission wavelength of 428 nm. When the berberine concentration is in the range of 0.1 - 80 μg / mL, there is a good linear relationship between the fluorescence quenching efficiency V / V0, and the linear equation is V / V0 = –7.51×10 -3 X + 0.99807, and the correlation coefficient R 2 = 0.9997, where V0 and V are the voltages of the mixed system before and after adding the berberine standard solution respectively, and X is the concentration of the berberine standard solution, with the unit μg / mL; Step S2, take 0.5 mL of a TPDCA fluorescent probe solution with a concentration of 7.5 μg / mL and add it to the sample solution containing berberine to be measured, then make up the volume to 5 mL with deionized water, mix evenly and react at 25 °C for 2.0 min. Use the portable fluorescence detection device to measure the voltage of the mixed system before and after adding the sample solution containing berberine to be measured at an excitation wavelength of 348 nm and an emission wavelength of 428 nm. Then, according to the measured voltage of the mixed system before and after adding the sample solution containing berberine to be measured, and combined with the linear equation obtained above, calculate the concentration of berberine in the sample solution containing berberine to be measured, with the unit μg / mL.

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