Determination method for insect repelling performance of storage insect pest resistant preparation

Abstract

The invention relates to the technical field of deinsectization preparations and grain preservation, in particular to a method for measuring the deinsectization performance of a storage pest resisting preparation. The method comprises six steps of S1 to S6, namely obtaining seeds of crops with insects, preparing experimental containers and centrifugal tubes, setting different treatment groups, burying the tubes, counting the number of insects, and calculating an average value of insect resistance through repeated experiments to obtain a final measurement result. The operation details of each step, such as seed source and treatment, seed volume requirement in a container, centrifuge tube specification and number, tube burying mode, artificial climate box temperature, humidity and time setting, insect resistance calculation formula and the like, are clearly stipulated. The determination method aims at objectively and accurately determining the insect repelling performance of the stored insect pest preparation through scientific design of an experimental process and accurate control of variables, and provides a reliable basis for research, development and application of related products.

Description

Technical Field

[0001] The present invention relates to the technical fields of insecticidal preparations and grain preservation technologies, and in particular to a method for measuring the insect repellent performance of an anti-stored grain pest preparation. Background Art

[0002] Although paddy rice has been cleaned before storage, the seeds themselves still carry a large number of microorganisms, and sometimes may also be accompanied by a small amount of stored grain pests. Rats and stored grain pests may sometimes enter the warehouse. There are many types of stored grain pests of seeds. In China, there are nearly 200 species, with beetles of Coleoptera being the majority, followed by moths (Lepidoptera). Stored grain pests cause great harm to seeds. They not only bore into the seeds and cause the seeds to lose viability, but also the large occurrence of stored grain pests will cause the seed pile to heat up, accelerate the aging of paddy rice, damage the seed warehouse, and seriously threaten the safety of seed storage. According to statistics, due to the aging of paddy rice itself and the influence of insects, microorganisms and other environmental factors, the paddy rice lost every year accounts for 10% of the total paddy rice inventory, causing economic losses of billions of yuan. Moreover, a large amount of water and heat are released, causing the seeds to heat up, change color, mildew, and become poisonous, resulting in a decline in the quality of the seeds.

[0003] In China, for national grain reserve warehouses, chemical control is the main means to reduce the harm of stored grain pests. Under sealed conditions, the use of some highly toxic fumigants such as phosphine has achieved good insecticidal effects. However, the long-term use of fumigants can not only cause an increase in the resistance of stored grain pests to insecticides, but also cause serious harm to the human body and the environment, and is also prone to cause secondary pollution of rice. In order to reduce environmental pollution and avoid harm to the human body, some researchers have proposed to use volatile substances of paddy rice to make attractants or insecticides for stored grain pests to replace some highly toxic fumigants such as phosphine. For the insect repellent performance of newly emerging and more environmentally friendly non-traditional anti-stored grain pest preparations, there is currently no relatively objective detection method and evaluation standard, which hinders the popularization and application of new anti-stored grain pest preparations. Summary of the Invention

[0004] In order to solve the technical problem in the prior art that the insect repellent performance of a new anti-stored grain pest preparation cannot be objectively and accurately judged, the method for measuring the insect repellent performance of the anti-stored grain pest preparation proposed by the present invention sets a mixed treatment of an anti-insect preparation and an attractant and a separate treatment of the anti-insect preparation, and includes the following steps:

[0005] A method for measuring the insect repellent performance of an anti-stored grain pest preparation, comprising the following steps:

[0006] S1: Take crop seeds with eggs, larvae and adults of stored grain pests;

[0007] S2: Put the crop seeds into an experimental container;

[0008] S3: Prepare several centrifuge tubes with lids and holes as insect-trapping containers for the sample group and the reference group. The number of centrifuge tubes in the sample group and the reference group should be the same:

[0009] One of the following two treatments is adopted for the sample group and the reference group:

[0010] Add the anti-stored-product pest preparation to the centrifuge tubes of the sample group, and leave the centrifuge tubes of the reference group blank; or: add a mixture of the anti-stored-product pest preparation and the attractant to the centrifuge tubes of the sample group, and add the attractant to the centrifuge tubes of the reference group;

[0011] S4: Bury the centrifuge tubes of the sample group and the reference group into the experimental containers filled with crop seeds;

[0012] S5: Cover the experimental containers, place them in an artificial climate chamber, regularly count the number of insects in the sample group and the reference group respectively, repeat multiple times, and calculate the average value of the number of insects in each group each time;

[0013] S6: Repeat steps S1 - S5 for 2 - 3 times. After each operation is completed, according to the average value of the number of insects in the two groups obtained in S5 this time, introduce the insect resistance calculation formula to calculate the insect resistance of the anti-stored-product pest preparation this time. After all repeated operations and calculations are completed, take the average value of the insect resistance each time as the final determination result of the insect resistance performance of this preparation.

[0014] Preferably, in S1, the crop seeds can be obtained from a warehouse where they have been stored for more than 2 years; or take crop seeds with stored-product pest eggs, sprinkle them evenly with water, place them in a bag or bucket at room temperature, and keep them in a dark place for 7 - 14 days.

[0015] Preferably, in S2, when the crop seeds are put into the experimental containers, the volume of the crop seeds should reach two-thirds of the volume of the experimental containers and the surface should be flat.

[0016] Preferably, the centrifuge tubes with lids and holes are provided with 4 holes horizontally and 7 holes vertically, the diameter of each hole is 1 - 1.5 mm, and the bottom of the centrifuge tubes with lids and holes is conical.

[0017] Preferably, in S3, the number of centrifuge tubes in the sample group and the reference group is 3, for a total of 6.

[0018] For the centrifuge tubes with lids and holes used as the sample group and the reference group, there are openings on their side walls and they are buried in the crop seeds in the experimental containers.

[0019] Preferably, in S4, when burying the centrifuge tubes of the sample group and the reference group into the experimental containers filled with crop seeds, the centrifuge tubes of the two groups need to be buried crosswise and evenly into the crop seeds.

[0020] Preferably, in S5, the temperature in the artificial climate chamber is between 25°C and 32.5°C, the humidity is set between 50% and 70%, and the placement time is 72 hours to 96 hours.

[0021] Preferably, in S6, the insect resistance calculation formula is as follows:

[0022]

[0023] Wherein, R is the insect repellency of the anti-stored grain pest preparation, N0 is the number of attracted insects in the sample group, and N1 is the number of attracted insects in the reference group.

[0024] The beneficial effects of the present invention are as follows:

[0025] 1. The insect repellency determination method proposed by the present invention can objectively and accurately judge the insect repellency of new anti-stored grain pest preparations, and effectively promote the popularization and application of new anti-stored grain pest preparations.

[0026] 2. The present invention sets up a comparison between the sample and the reference group, considers the single use of the insect-resistant preparation and its mixture with the attractant, repeats multiple times and calculates the average number of insect heads, greatly reducing errors, accurately evaluating the insect repellency performance, and making up for the deficiencies of the existing technology.

[0027] 3. The present invention selects specific seeds, regulates the state of the seeds in the experimental container, sets the temperature, humidity and duration of the artificial climate chamber, highly restores the storage environment, makes the measurement results conform to the actual application, and has high guiding value.

[0028] 4. The present invention clarifies the number of holes, hole diameter and bottom shape of the capped perforated centrifuge tube, stipulates the number and embedding method of the centrifuge tubes in the sample and reference groups, ensures consistent and repeatable experimental conditions, reduces errors, and improves scientificity.

[0029] 5. The insect resistance calculation formula of the present invention is based on the number of attracted insects in the sample and reference groups, is simple to calculate, can intuitively quantify the experimental data into insect resistance indicators, and is convenient for scientific research and practical application personnel to understand and use. BRIEF DESCRIPTION OF THE DRAWINGS

[0030] Figure 1 is a flowchart of the insect repellency determination method in the embodiment of the present invention;

[0031] Figure 2 is a structural schematic diagram of the capped perforated centrifuge tube in the embodiment of the present invention;

[0032] Figure 3 is a schematic diagram of the setting of the capped perforated centrifuge tube in the experimental container in the embodiment of the present invention;

[0033] Figure 4 is a top view of the setting method of the centrifuge tube in the experimental container in Embodiment 1 of the present invention;

[0034] Figure 5 It is a comparison chart of the number of attracted insects between the centrifuge tubes containing anti-stored-product pest agents and the centrifuge tubes without anti-stored-product pest agents in the first embodiment of the present invention;

[0035] Figure 6 It is a top view of the setting method of the centrifuge tube in the experimental container in the second embodiment of the present invention;

[0036] Figure 7 It is a comparison chart of the number of attracted insects between the centrifuge tubes containing anti-stored-product pest agents and the centrifuge tubes without anti-stored-product pest agents in the second embodiment of the present invention. Detailed implementation manners

[0037] A method for measuring the insect repellent performance of an anti-stored-product pest agent, comprising the following steps:

[0038] S1: Take out crop seeds from a warehouse that has been stored for more than 2 years; or select crop seeds with stored-product pest eggs, sprinkle them evenly with water, then place them in a bag or a bucket, and store them in a dark place at room temperature for 7 to 14 days, and then take them out; thus, crop seeds with stored-product pest eggs, larvae and adults can be obtained.

[0039] S2: Put the crop seeds into the experimental container, and the volume of the crop seeds should reach two-thirds of the volume of the experimental container and the surface should be flat.

[0040] S3: Prepare 6 centrifuge tubes with 4 holes horizontally and 7 holes vertically, each hole having a diameter of 1 - 1.5 mm, and the bottom being conical with a lid and holes, as the insect-attracting containers for the sample group and the reference group. The number of centrifuge tubes in the sample group and the reference group should be the same, each being 3. Then:

[0041] One of the following two treatments is adopted for the sample group and the reference group:

[0042] Add the anti-stored-product pest agent to the centrifuge tubes of the sample group, and leave the centrifuge tubes of the reference group blank; or: add a mixture of the anti-stored-product pest agent and the attractant to the centrifuge tubes of the sample group, and add the attractant to the centrifuge tubes of the reference group; the attractant is diatomaceous earth.

[0043] S4: Cross and evenly bury the centrifuge tubes of the sample group and the reference group into the crop seeds in the experimental container.

[0044] S5: Cover the experimental container, put it into an artificial climate chamber, set the temperature between 25°C and 32.5°C, set the humidity between 50% and 70%, regularly count the number of insects in the sample group and the reference group respectively, repeat multiple times, and end the statistics after 96 hours of placement and calculate the average value of the number of insects in each group each time;

[0045] S6: Repeat steps S1 - S5 two to three times. After each operation is completed, according to the average value of the number of insect heads obtained in S5 for that time, introduce the insect resistance calculation formula to calculate the insect resistance of the anti - storage pest preparation for that time. The insect resistance calculation formula is as follows:

[0046]

[0047] R is the insect repellency of the anti - storage pest preparation, N0 is the number of attracted insects in the sample group, and N1 is the number of attracted insects in the reference group.

[0048] After all repeated operations and calculations are completed, take the average value of the insect resistance for each time as the final determination result of the insect resistance performance of this preparation.

[0049] Example 1

[0050] In this example, the insect repellency performance of the mixture of the anti - storage pest preparation and the attractant was measured. In the specific experimental process, the anti - storage pest preparation was derived from the national invention patent ZL202010285824.3, and the attractant was diatomaceous earth of commercially available analytical pure grade.

[0051] The experimental container was a covered plastic bucket with a barrel mouth diameter of 29 cm and a height of 25.5 cm. The specific experimental process included:

[0052] Take wheat seeds with a large number of storage pest eggs, larvae and adults, place them in the plastic bucket until the volume reaches two - thirds and the surface is flat. Then, place 0.2 g of diatomaceous earth attractant without the anti - storage pest preparation and diatomaceous earth containing the anti - storage pest preparation (0.2 g of diatomaceous earth attractant and 8% content of the anti - storage pest preparation) at the bottom of 10 - ml capped and perforated centrifuge tubes respectively.

[0053] Then, insert the centrifuge tubes with 0.2 g of diatomaceous earth attractant without the anti - storage pest preparation and diatomaceous earth containing the anti - storage pest preparation (0.2 g of diatomaceous earth attractant and 8% content of the anti - storage pest preparation) placed at the bottom into the plastic bucket containing wheat seeds respectively, and immerse all the holes of the capped and perforated centrifuge tubes into the wheat seeds. The setting method of the centrifuge tubes is as Figure 4 shown, where A1, C1, E1 are capped and perforated centrifuge tubes with the mixture of the anti - storage pest preparation and the attractant placed at the bottom, and B1, D1, F1 are capped and perforated plastic centrifuge tubes with the attractant placed at the bottom.

[0054] For the same experiment, 2 groups were set up, with 3 centrifuge tubes in each group for replication. A total of 6 centrifuge tubes were placed crosswise and evenly. The plastic bucket was covered and placed in an artificial climate chamber. The temperature was set at 32.5 °C, the humidity was set at 60%, and the placement time was 72 hours. The number of insect heads in different centrifuge tubes was investigated regularly, and the average value was taken. This experiment was repeated 3 times. According to the insect resistance calculation formula in S6 above, the insect resistance was calculated and then the average value was taken, which represented the final determination result of the insect resistance performance of the preparation.

[0055]

[0056] Table 1

[0057] From Table 1 and Appendix Figure 5 It can be seen that the average number of adult insects trapped by the 0.2 g diatomaceous earth treatment containing 8% insect-resistant preparation was 236.3, while the average number of adult insects trapped by the 0.2 g diatomaceous earth treatment without 8% insect-resistant preparation was 326.0, which was 89.7 higher than the average number of adult insects trapped by the 0.2 g diatomaceous earth treatment containing 8% insect-resistant preparation. Obviously, the presence of the insect-resistant preparation affected the attracting performance of the diatomaceous earth attractant for adult insects. However, for larvae, the average number of larvae trapped by the 0.2 g diatomaceous earth treatment containing 8% insect-resistant preparation was 61.3, and the average number of larvae trapped by the 0.2 g diatomaceous earth treatment without 8% insect-resistant preparation was 52.7, which was 8.6 lower than the average number of larvae trapped by the 0.2 g diatomaceous earth treatment containing 8% insect-resistant preparation. Therefore, the presence of the insect-resistant preparation did not affect the attracting performance of the diatomaceous earth attractant for larvae.

[0058] The insect resistance calculation formula is as follows:

[0059]

[0060] Among them, R is the insect repellency of the anti-stored grain pest preparation, N0 is the number of trapped insects in the sample group (the mixture of the anti-stored grain pest preparation and the attractant), and N1 is the number of trapped insects in the reference group (the attractant).

[0061] The anti-stored grain pest preparation was added to the centrifuge tubes in the sample group, and the centrifuge tubes in the reference group were blank;

[0062] The calculation results obtained according to the insect resistance calculation formula in S6 above are shown in Table 2. It can be seen from Table 2 that the insect repellency of the insect-resistant preparation against adult insects was 27.5, with obvious repellency, and the insect repellency of the insect-resistant preparation against larvae was -16.5, without repellency.

[0063]

[0064] Table 2

[0065] Example 2

[0066] In this example, the insect repellent performance of the anti-warehouse pest preparation was measured. During the specific experiment, the anti-warehouse pest preparation was derived from the national invention patent ZL202010285824.3.

[0067] The experimental container was a plastic bucket with a lid, with a bucket mouth diameter of 29 cm and a height of 25.5 cm. The specific experimental process included:

[0068] Take wheat seeds with a large number of warehouse pest eggs, larvae and adults, place them in a plastic bucket, and make the volume reach two-thirds and the surface flat. Then, insert a blank capped perforated plastic centrifuge tube with no anti-warehouse pest preparation at the bottom and a capped perforated plastic centrifuge tube with 50 mg of anti-warehouse pest preparation at the bottom into the plastic bucket containing wheat seeds, and then submerge all the holes of the centrifuge tube into the wheat seeds. The setting method of the centrifuge tube is as Figure 6 shown, where A2, C2, and E2 are centrifuge tubes with anti-warehouse pest preparation at the bottom, and B2, D2, and F2 are blank centrifuge tubes.

[0069] Set 2 groups for one test, with 3 centrifuge tubes repeated in each group, a total of 6 tubes placed crosswise and evenly. Cover the plastic bucket and place it in an artificial climate chamber. Set the temperature to 32.5 °C, the humidity to 60%, and the placement time to 72 hours. Regularly investigate the number of insect heads in different centrifuge tubes and take the average value. This test was repeated 3 times, and the insect resistance was calculated according to the insect resistance calculation formula in S6 above and then the average value was taken, which represented the final measurement result of the insect resistance of the preparation.

[0070]

[0071]

[0072] Table 3

[0073] From Table 3 and the appendix Figure 7 It can be seen that the average number of adult insects treated with 50 mg of pure insect repellent preparation was 27.7, while the average number of adult insects in the blank control without insect repellent preparation was 36.6, which was 8.9 higher than the average number of adult insects treated with 50 mg of pure insect repellent preparation. Obviously, the presence of the insect repellent preparation affected the activity of stored pests. However, for larvae, the average number of larvae treated with 50 mg of pure insect repellent preparation was 3.0, while the average number of larvae in the blank control without insect repellent preparation was 2.3, which was 0.7 lower than the average number of larvae treated with 50 mg of pure insect repellent preparation. Therefore, the presence of the insect repellent preparation did not affect the attracting performance of the diatomaceous earth attractant for larvae.

[0074] The insect resistance calculation formula is as follows:

[0075]

[0076] Among them, R is the insect repellency of the anti-stored grain pest preparation, N0 is the number of attracted insects in the sample group (anti-stored grain pest preparation), and N1 is the number of attracted insects in the reference group (blank).

[0077] The calculation results obtained according to the insect resistance calculation formula in S6 above are shown in Table 4. It can be seen from Table 4 that the insect resistance of the insect-resistant preparation against adult insects is 24.3, and the insect resistance is obvious. The insect resistance of the insect-resistant preparation against larval insects is -28.6, indicating no insect resistance.

[0078]

[0079] Table 4

[0080] Both Example 1 and Example 2 can to a certain extent reflect the insect repellent performance of the anti-stored grain pest preparation against adult stored grain pests. However, comparatively speaking, the anti-stored grain pest preparation in Example 1 has a larger number of adult insects with the assistance of the attractant, and the difference is more obvious. Therefore, by using the mixture of the attractant and the insecticide, the measured results of the insect repellent performance of the insect-resistant preparation against stored grain pests are more accurate and reliable.

[0081] The above-disclosed are only the preferred embodiments of the present invention, and of course, the scope of rights of the present invention cannot be limited thereby. It should be pointed out that for those skilled in the art of this technology, any equivalent changes made to the present invention without departing from the design structure and principle of the present invention shall be regarded as the protection scope of the present invention.

Claims

1. A method for determining the insect repellent performance of an anti-storage pest preparation, characterized in that: The following steps are involved: S1: Take crop seeds with eggs, larvae and adults of stored pests; S2: Place crop seeds into experimental containers; S3: Prepare several centrifuge tubes with lids and holes as insect trap containers for the sample group and the reference group: The sample group and reference group were treated in one of the following two ways: Add the anti-storage pest preparation to the centrifuge tube of the sample group, and leave the centrifuge tube of the reference group blank; or: add the mixture of the anti-storage pest preparation and the attractant to the centrifuge tube of the sample group, and add the attractant to the centrifuge tube of the reference group; S4: bury the centrifuge tubes of the sample group and the reference group into the experimental container containing crop seeds; S5: Cover the experimental container and place it in an artificial climate chamber. Regularly count the number of insect heads in the sample group and the reference group. Repeat several times and calculate the average number of insect heads in the two groups. S6: Repeat steps S1-S5 2-3 times. After each operation is completed, the insect resistance calculation formula is introduced to calculate the insect resistance of the anti-storage pest preparation according to the average value of the number of insect heads in the two groups obtained in S5. After all repeated operations and calculations are completed, the average value of each insect resistance is taken as the final determination result of the insect resistance of the preparation.

2. The method for determining the insect repellent performance of an anti-storage pest preparation according to claim 1, characterized in that: In S1, the crop seeds can be obtained from a warehouse where they have been stored for more than 2 years; or the crop seeds with stored pest eggs can be sprinkled with water evenly, placed in a bag or barrel at room temperature, and placed in a dark place for 7-14 days.

3. The method for determining the insect repellent performance of an anti-storage pest preparation according to claim 1, characterized in that: In the above-mentioned S2, when the crop seeds are placed in the experimental container, the volume of the crop seeds must reach two-thirds of the experimental container and the surface must be flat.

4. The method for determining the insect repellent performance of an anti-storage pest preparation according to claim 1, characterized in that: The centrifuge tube with a cover and holes is provided with 4 transverse holes and 7 longitudinal holes, and the diameter of each hole is 1-1.5 mm.

5. The method for determining the insect repellent performance of an anti-storage pest preparation according to claim 1, characterized in that: In the S3, the number of centrifuge tubes for the sample group and the reference group is 3, totaling 6.

6. The method for determining the insect repellent performance of an anti-storage pest preparation according to claim 1, characterized in that: In the above-mentioned S4, when the centrifuge tubes of the sample group and the reference group are buried in the experimental container containing the crop seeds, the centrifuge tubes of the two groups need to be evenly buried in the crop seeds in a cross-like manner.

7. The method for determining the insect repellent performance of an anti-storage pest preparation according to claim 1, characterized in that: In the S5, the temperature in the artificial climate box is between 25°C and 32.5°C, the humidity is set between 50% and 70%, and the placement time is 72 hours to 96 hours.

8. The method for determining the insect repellent performance of an anti-storage pest preparation according to claim 1, characterized in that: The weight of the attractant in the mixture of the anti-storage pest preparation and the attractant is equivalent to that of the attractant added to the centrifuge tube of the reference group, and the attractant is diatomaceous earth.

9. The method for determining the insect repellent performance of an anti-storage pest preparation according to claim 1, characterized in that: The centrifuge tubes with lids and holes used as the sample group and the reference group have openings on their side walls and are buried in the crop seeds in the experimental container.

10. The method for determining the insect repellent performance of an anti-storage pest preparation according to claim 1, characterized in that: In the above-mentioned S6, the insect resistance calculation formula is as follows: ; Where R is the insect repellency of the anti-storage pest preparation, N0 is the number of insects trapped in the sample group, and N1 is the number of insects trapped in the reference group.

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