Fluopyram-containing bactericidal composition

Abstract

The invention relates to a sterilization composition containing fluopyram, in particular to application of the sterilization composition containing fluopyram and iprodione to prevention and treatment of cucumber downy mildew, and the composition is mainly characterized in that the weight ratio of fluopyram to iprodione is 1: 1-1: 12, the composition is prepared into a suspending agent, and the suspending agent is a suspending agent. The dosage of the effective active ingredients is 30-120 g / hectare.

Description

Technical Field

[0001] The present invention belongs to the technical field of pesticides, and particularly relates to a bactericidal composition containing fluopyram. The bactericidal composition is composed of fluopyram, iprodione, and auxiliaries. Background Art

[0002] Fluopyram is a nematicide with a brand-new mechanism of action. It belongs to the succinate dehydrogenase inhibitor (SDHI) class and has strong nematicidal activity. Its mechanism of action: inhibits the activity of the target succinate dehydrogenase, thereby interfering with its respiratory function. When nematodes are treated with fluopyram, the worm bodies become rigid like needles and their motility drops sharply. For example, symptoms initially appear 30 minutes after treatment. For Radopholus similis or Meloidogyne incognita, after being treated with the drug, their activities start to slow down and they become completely paralyzed and immobile after 1 - 2 hours. Fluopyram selectively inhibits Complex II of the respiratory chain in nematode mitochondria. Mitochondria are the energy factories of nematodes, and when it is inhibited, it will cause the energy (ATP) in nematode cells to be quickly depleted. Fluopyram is the first nematicide that inhibits Complex II, and it represents a new class of nematicides with a mechanism of action.

[0003] Iprodione is a highly efficient, broad-spectrum, contact fungicide of the dicarboximide class. It acts on spores, mycelia, and sclerotia simultaneously, inhibiting the germination of fungal spores and the growth of mycelia. It can hardly penetrate into plants and belongs to a protective fungicide. This agent has good bactericidal effects on Botrytis cinerea, Sclerotinia, Monilinia, Sclerotium, and Monilia.

[0004] The inventors found through experiments that by supporting a suspension composition with fluopyram and iprodione with different mechanisms of action in a certain proportion, the control effect on cucumber downy mildew can be effectively improved, the control cost can be reduced, and it is highly safe for crops. Summary of the Invention

[0005] The purpose of the present invention is to provide a bactericidal composition containing fluopyram.

[0006] The technical solution of the present invention is as follows:

[0007] A bactericidal composition containing fluopyram, characterized in that: the effective active ingredients of the bactericidal composition are fluopyram and iprodione, and the weight ratio of fluopyram to iprodione is 1:1 - 1:12, preferably 1:23 - 1:9, and the best weight ratio is 1:6.

[0008] Furthermore, the bactericidal composition contains fluopyram, iprodione, a dispersant, a wetting agent, an antifreeze, a thickener, an antifoaming agent, a preservative, and water, and the composition is made into a suspension.

[0009] Furthermore, the total weight of fluxapyroxad and iprodione in the bactericidal composition is 10% - 50%, and preferably the total weight is 40%.

[0010] The dispersant is selected from one or more of alkylphenol polyoxyethylene ether formaldehyde condensate sulfate, alkylphenol polyoxyethylene ether formaldehyde condensate phosphate, sodium methylene bisnaphthalene sulfonate, sodium or calcium lignosulfonate, melamine formaldehyde resin, alkylnaphthalene sulfonate, EO-PO block polyether, styrene phenol polyoxyethylene ether phosphate, alkylnaphthalene formaldehyde condensate sulfonate, alkylphenol polyoxyethylene ether phosphate ester, alkylphenol polyoxyethylene ether sulfonate, phenethylphenol polyoxyethylene ether phosphate ester, alkyl polyoxyethylene ether sulfonate, polyoxyethylene polyoxypropylene block copolymer, dodecyl polyoxyethylene ether phosphate ester, fatty alcohol polyoxyethylene ether phosphate ester, nonylphenol polyoxyethylene ether phosphate ester, sodium naphthalene sulfonic acid formaldehyde condensate block copolymer, comb-shaped polycarboxylate, sodium polycarboxylate;

[0011] The wetting agent is selected from one or more of sodium dodecylbenzenesulfonate, sodium diisobutylnaphthalenesulfonate, sorbitan fatty acid ester, sodium sulfosuccinate octyl ester, alkyl sulfosuccinate, phenethylphenol polyoxyethylene ether phosphate ester, alkyl sulfate, alkyl sulfonate, alkylnaphthalene sulfonate, alkylphenol formaldehyde resin polyoxyethylene ether, triphenylethylphenol polypropylene oxide polyoxyethylene block polymer, sodium dodecyl sulfate, styrylphenol formaldehyde resin propylene oxide block polyether, castor oil polyoxyethylene ether, fatty alcohol polyoxyethylene ether, alkyl glycoside, sodium fatty alcohol polyoxyethylene ether sulfonate;

[0012] The defoamer is selected from one or more of silicone, fatty alcohol, fatty acid;

[0013] When the bactericidal composition is made into a suspension agent, it contains the following components and contents: fluxapyroxad 5% - 20%, iprodione 5% - 40%, dispersant 1% - 10%, wetting agent 1% - 10%, antifreeze 1% - 8%, defoamer 0 - 2%, thickener 0 - 2%, preservative 0 - 2%, and water is added to 100%.

[0014] Furthermore, the bactericidal composition is used to control cucumber downy mildew, and the dosage of the active ingredient is 228 - 342 g / ha.

[0015] Compared with the prior art, the bactericidal composition for controlling cucumber downy mildew of the present invention has the following beneficial effects: (1) Compared with the single agent, the composition has a more significant effect on controlling cucumber downy mildew; (2) The mixing of two compounds with different action mechanisms can effectively delay the generation of disease resistance, and has good safety and is safe for cucumbers. Specific embodiments

[0016] The present invention will be further described below in conjunction with embodiments. All percentages in the embodiments are weight percentages, but the present invention is not limited thereto.

[0017] Application Example 1

[0018] Example 1 35% Fluopyram·Iprodione Suspension Concentrate

[0019] 5% fluopyram technical, 30% iprodione technical, 8% alkoxy-modified polytrioxane, 2% sodium naphthalene sulfonate formaldehyde condensate block copolymer, 2% fatty alcohol polyoxyethylene ether phosphate, 2% styrylphenol formaldehyde resin propylene oxide block polyether, 3% castor oil polyoxyethylene ether, 5% propylene glycol, 1% sodium benzoate, 1% magnesium aluminum silicate, 0.7% glacial acetic acid, 0.2% C10-20 saturated fatty acid compound, and water added to 100% to obtain 35% fluopyram·iprodione suspension concentrate.

[0020] Example 2 20% Fluopyram·Iprodione Suspension Concentrate

[0021] 5% fluopyram technical, 15% iprodione technical, 5% mixture of polyethoxy-modified silane and polyethoxy-modified fatty alcohol, 1% polyoxyethylene polyoxypropylene block copolymer, 2% nonylphenol polyoxyethylene ether phosphate, 2% alkyl sulfate, 2% triphenylethylphenol polyoxypropylene polyoxyethylene block polymer, 0.5% silicone defoamer, 0.8% xanthan gum, 4% ethylene glycol, 1% sodium benzoate, and water added to 100% to obtain 20% fluopyram·iprodione suspension concentrate.

[0022] Example 3 50% Fluopyram·Iprodione Suspension Concentrate

[0023] 5% fluopyram technical, 45% iprodione technical, 4% alkoxy-modified polytrioxane, 3% alkylphenol polyoxyethylene ether sulfonate, 3% phenethylphenol polyoxyethylene ether phosphate, 4% alkylnaphthalene sulfonate, 15% polyethylene glycol, 0.5% sodium benzoate, 0.5% organosilicone defoamer, 0.5% C8-10 fatty alcohol, and water added to 100% to obtain 50% fluopyram·iprodione suspension concentrate.

[0024] Application Example 2:

[0025] Toxicity determination of the mixture of iprodione and fluopyram against Pseudoperonospora cubensis

[0026] According to the standards of NY / T1156.3-2006 and NY / T1156.6-2006, the toxicity of fluopyram, iprodione and their mixtures at ratios of 1:1, 1:3, 1:6, 1:9 and 1:12 against Pseudoperonospora cubensis was determined by the petri dish leaf method. The results showed that the mixtures of fluopyram and iprodione at the above 5 ratios showed synergistic effects on Pseudoperonospora cubensis. The experimental process is as follows:

[0027] 1 Test purpose

[0028] The toxicity of fluopyram, iprodione and their mixtures at different ratios against Pseudoperonospora cubensis was determined indoors, and the synergistic effect was evaluated to clarify their compatibility and provide a scientific basis for the research and development of the mixture of fluopyram and iprodione.

[0029] 2 Test conditions

[0030] 2.1 Test target

[0031] Pseudoperonospora cubensis (Berk. et Curt.) Rostovzev, preserved and provided by Hunan Aimiao Testing Co., Ltd.

[0032] 2.2 Culture conditions

[0033] The culture conditions for the test target and the target after the test were a temperature of 17-22°C and a relative humidity of over 90%.

[0034] 2.3 Instrument and equipment

[0035] Beakers, conical flasks, pipettes, measuring cylinders, hemocytometers, microscopes, crop sprayers, ten-thousandth electronic balances, etc.

[0036] 3 Test design

[0037] 3.1 Test agents

[0038] 98% fluopyram technical; 98% iprodione technical.

[0039] 3.2 Design of agent concentration and preparation of solution

[0040] Fludioxonil solution: Weigh 0.0102 g of 98% fludioxonil technical with a sterilized small beaker, dissolve it with 0.5 mL of DMF, add a small amount of sterile water containing 0.1% Tween 80 emulsifier, stir evenly, transfer it to a 100 mL volumetric flask, then rinse the small beaker 3 times with an appropriate amount of sterile water containing 0.1% Tween 80 emulsifier, transfer the rinsing solutions to the volumetric flask, and finally make up the volume to 100 mL to prepare a 100 mg / L stock solution. Then dilute it with sterile water containing 0.1% Tween 80 emulsifier to concentrations of 10, 5, 1, 0.5, 0.1, 0.05 mg / L for standby;

[0041] Iprodione solution: Weigh 0.0102 g of 98% iprodione technical with a sterilized small beaker, dissolve it with 0.5 mL of DMF, add a small amount of sterile water containing 0.1% Tween 80 emulsifier, stir evenly, transfer it to a 100 mL volumetric flask, then rinse the small beaker 3 times with an appropriate amount of sterile water containing 0.1% Tween 80 emulsifier, transfer the rinsing solutions to the volumetric flask, and finally make up the volume to 100 mL to prepare a 100 mg / L stock solution. Then dilute it with sterile water containing 0.1% Tween 80 emulsifier to concentrations of 50, 20, 10, 5, 1 mg / L for standby;

[0042] Each mixed solution: Mix fludioxonil and iprodione in the ratios of 1:1, 1:3, 1:6, 1:9, and 1:12, and then dilute it with sterile water containing 0.1% Tween 80 emulsifier to concentrations of 10, 5, 1, 0.5, 0.1, 0.05 mg / L for standby;

[0043] Control solution: 0.5 mL of DMF plus 95 mL of sterile water containing 0.1% Tween 80 emulsifier.

[0044] 4 Test methods

[0045] Refer to the bioassay standard method NY / T1156.3 - 2006 and adopt the petri dish leaf method. Preparation of sporangium suspension: Select diseased leaves, wash the sporangia of downy mildew on the back of the leaves with distilled water at 4°C to prepare a suspension (the concentration is controlled at 1×105 - 1×107 sporangia per milliliter), and store it at 4°C for standby.

[0046] Spray the prepared liquid medicine evenly on the back of the leaves. After the liquid medicine dries naturally, place the back of each treated leaf upwards, arrange them in a moisture box according to the treatment marks. 24 hours after the chemical treatment, drop 10 μL of the prepared fresh sporangium suspension onto the back of the leaves. Inoculate 4 drops on each leaf, and use 5 leaves for each treatment. Set up a treatment without the medicine as a blank control. After inoculation, cover the petri dish lid and place it in an artificial climate chamber, and culture it under the conditions of 12 h of continuous light / dark alternation per day, a temperature of 17°C - 22°C, and a relative humidity of more than 90%.

[0047] 5 Data Investigation and Statistical Analysis

[0048] 5.1 Investigation Method

[0049] Measure and record the lesion diameter according to the disease incidence of the blank control, and calculate the control effect (%).

[0050]

[0051] Where: I represents the mycelial growth inhibition rate; D0 represents the colony growth diameter of the blank control; Dt represents the colony growth diameter of the medicament treatment.

[0052] 5.2 Evaluation Method for Synergistic Effect

[0053] Refer to the Guidelines for Indoor Bioassay of Pesticides NY / T1156.6-2006, and evaluate the synergistic effect of the mixed agents according to the co-toxicity coefficient method (CTC) of Sun&Johnson (1960), that is, CTC≤80 is antagonistic effect, 80<CTC<120 is additive effect, and CTC≥120 is synergistic effect.

[0054]

[0055] The theoretical toxicity index (TTI) of the mixture = the toxicity index of agent A × the percentage of agent A in the mixture (%) + the toxicity index of agent B × the percentage of agent B in the mixture (%)

[0056]

[0057]

[0058] 5.3 Data Statistical Analysis

[0059] All experimental data were analyzed using DPS v9.50 statistical software. Calculate the mycelial growth inhibition rate (%) according to the experimental data, and obtain the toxicity regression equation, correlation coefficient (r) and EC50 (95% confidence limit).

[0060] 6 Result Analysis and Discussion

[0061] 6.1 Efficacy Evaluation

[0062] The results of the toxicity determination of the mixed combinations of different ratios of fluxapyroxad and iprodione against Pseudoperonospora cubensis are shown in Table 1. As can be seen from Table 1, the mixtures of fluxapyroxad and iprodione at the ratios of 1:1, 1:3, 1:6, 1:9, and 1:12 all showed synergistic effects against Pseudoperonospora cubensis, with their EC50 values being 0.56, 0.49, 0.43, 0.75, and 0.71 mg / L respectively, and their co-toxicity coefficients being 139.44, 134.17, 147.17, 140.44, and 136.36 respectively. Among them, the mixture at the ratio of 4:15 had a better synergistic effect.

[0063] Table 1 Results of the toxicity determination of the mixture of fluxapyroxad and iprodione against Pseudoperonospora cubensis

[0064]

[0065] 6.2 Discussion and conclusions

[0066] The results of the indoor bioassay showed that the mixtures of fluxapyroxad and iprodione at the ratios of 1:1, 1:3, 1:6, 1:9, and 1:12 all showed synergistic effects against Pseudoperonospora cubensis, and among them, the synergistic effect of the 1:6 ratio was better. Therefore, 1:6 can be regarded as the optimal mixing ratio of fluxapyroxad and iprodione.

[0067] Application Example 3: Field efficacy experiment of Examples 1 - 3 for controlling Pseudoperonospora cubensis on cucumbers

[0068] The test referred to the "Quality Control Code for Field Efficacy Trials of Pesticide Registrations" (NY / T 2885 - 2016) and the "Guidelines for Field Efficacy Trials of Pesticides (Part 1) - Section 26: Fungicides for Controlling Pseudoperonospora cubensis on Cucumbers" (GB / T 17980.26 - 2000). The test process was carried out strictly in accordance with the test plan, and this report truly and accurately reflects the test results.

[0069] Test crop: Cucumber

[0070] Target for control: Pseudoperonospora cubensis

[0071] 1 Basic information of the test

[0072] 1.1 Test name

[0073] Field efficacy test of fluxapyroxad·iprodione suspension concentrate against Pseudoperonospora cubensis on cucumbers.

[0074] 1.2 Test purpose

[0075] To clarify the control effect, applicable dosage, and safety of the fluxapyroxad·iprodione suspension concentrate provided by the applicant against Pseudoperonospora cubensis, and to provide a basis for the registration and promotion of the test agent.

[0076] 2 Environmental and facility cultivation conditions

[0077] 2.1 Test Location

[0078] Xinxiang City, Henan Province.

[0079] 2.2 Test Target Conditions

[0080] Cucumber downy mildew (Fusarium graminearum Schw.)

[0081] 2.3 Test Crops, Varieties and Growth Conditions

[0082] Cucumber, variety is Xinyou 36, sowing date is August 9, 2020, sowing method is dibbling, plant spacing is 25 cm, row spacing is 50 cm.

[0083] 3 Test Design and Arrangement

[0084] 3.1 Test Dosage and Number

[0085] Table 2 Test Design of Test Agents

[0086] Treatment number Agent Dosage of preparation (g / mu) Amount of active ingredient (g / hm2) 1 35% Fluopyram·Iprodione SC 5.7 30 2 20% Fluopyram·Iprodione SC 10 30 3 50% Fluopyram·Iprodione SC 4 30 4 10% Fluopyram SC 20 30 5 50% Iprodione WP 20 150 6 Water control —— ——

[0087] 3.2 Application Method

[0088] 3.2.1 Application Period and Method

[0089] A total of 3 applications were made, with an interval of 8 days between the three applications. The first application was on August 28, 2022, when the cucumber growth stage was in the seedling stage; the second application was on September 5, 2022, when the cucumber growth stage was in the early flowering stage; the third application was on September 13, 2022, when the cucumber growth stage was in the fruiting stage, by spraying.

[0090] 3.2.2 Application Volume

[0091] The amount of liquid medicine sprayed per hectare was about 750 liters.

[0092] 3.3 Investigation Method, Time and Frequency

[0093] 3.3.1 Investigation Time and Frequency

[0094] Before the first application (August 28) and before the second application (September 5), the cucumber downy mildew occurred sporadically and no disease index investigation was carried out. Investigations were carried out before the third application (September 13) and 8 days after the third application (September 21), with a total of 2 investigations.

[0095] 3.3.2 Investigation Method

[0096] Randomly select 4 points for investigation in each plot, randomly investigate 2 plants at each point, investigate all the leaves of each plant, and record according to the following grading method.

[0097] Level 0: No lesions;

[0098] Level 1: Lesion area less than 5% of the total leaf area;

[0099] Level 3: Lesion area 6% - 10% of the total leaf area;

[0100] Level 5: Lesion area 11% - 25% of the total leaf area;

[0101] Level 7: Lesion area 26% - 50% of the total leaf area;

[0102] Level 9: Lesion area more than 50% of the total leaf area.

[0103] 3.3.3 Efficacy calculation method

[0104]

[0105]

[0106] Note: CK0 - Disease index of the blank control area; PT0 - Disease index of the treatment area.

[0107] 4 Results and analysis

[0108] Table 3 Test results of Examples 1, 2, and 3 for controlling cucumber downy mildew

[0109]

[0110] The experimental results show that the control effects of the 35% fluxapyroxad·iprodione suspension concentrate, 20% fluxapyroxad·iprodione suspension concentrate, and 50% fluxapyroxad·iprodione suspension concentrate with an active ingredient dosage of 30 g / ha invented by the applicant are all higher than 80%, and the control effects are all significantly better than the control single agent.

[0111] The above content is a further detailed description of the present invention in combination with specific preferred embodiments. It cannot be determined that the specific implementation of the present invention is only limited to these descriptions. For those of ordinary skill in the technical field to which the present invention belongs, without departing from the concept of the present invention, several simple deductions or substitutions can be made, which should all be regarded as belonging to the protection scope of the present invention.

Claims

1. A bactericidal composition containing fluxapyroxad, characterized in that: The effective active ingredients of the bactericidal composition are fluxapyroxad and iprodione, and the weight ratio of fluxapyroxad to iprodione is 1:1 to 1:

12.

2. The bactericidal composition according to claim 1, characterized in that: The bactericidal composition contains fluxapyroxad, iprodione, synergist and silica white mixture, and adjuvant, and the composition is made into a suspension concentrate.

3. The bactericidal composition according to claim 1 or 2, wherein: The weight ratio of fluxapyroxad to iprodione is 1:

6.

4. The bactericidal composition according to any one of claims 1-3, characterized in that: The total weight of fluxapyroxad and iprodione in the bactericidal composition is 10% - 50%.

5. The bactericidal composition according to claim 4, characterized in that: The total weight of fluxapyroxad and iprodione in the bactericidal composition is 40%.

6. The bactericidal composition according to claim 2, wherein: The adjuvant is selected from one or more of dispersant, wetting agent, binder, defoamer, pH regulator, filler.

7. The bactericidal composition according to any one of claims 1 to 6, characterized in that: The bactericidal composition is used to control cucumber downy mildew.

8. The bactericidal composition according to claim 7, wherein: The dosage of the active ingredient of the bactericidal composition is 30 - 120 g / hectare.

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