Sesame paste not prone to floating oil and caking and preparation method of sesame paste

Abstract

The invention relates to sesame paste with improved stability and a preparation method thereof. Specifically, the invention provides the preparation method of the sesame extract, and the method comprises the following steps: drying sesame raw material residues obtained through oil removal treatment to obtain a solid component A, then carrying out alcohol extraction on the solid component A to obtain an alcohol-soluble component, extracting the alcohol-soluble component to obtain a water-layer extract B, and carrying out water-layer extraction on the water-layer extract B to obtain the sesame extract. The component A and the component B are prepared into an extract C according to a ratio of 1: 1-1: 3, namely the sesame extract, and the volume concentration of the aqueous solution of the alcohol is greater than or equal to 70%. The invention also provides a preparation method and application of the sesame extract. The sesame extract can improve the stability of the sesame paste, and the sesame paste is not prone to floating oil and caking.

Description

Technical Field

[0001] The present invention mainly relates to the technical field of food flavor sauce processing, and particularly relates to sesame paste with improved stability and a preparation method thereof. Background Art

[0002] Sesame, also known as flaxseed and oilseed, belongs to the Pedaliaceae family and the Sesamum genus, and is an annual herbaceous plant. Sesame is rich in nutritional value. The average oil content is 54.74%. The fatty acids are mainly composed of two unsaturated fatty acids, oleic acid and linoleic acid, with contents of 38.39% and 46.26% respectively. Linoleic acid can play a role in inhibiting the formation of arterial thrombosis and reducing serum cholesterol. The protein content in sesame is about 22.12%, and the content of essential amino acids for the human body is high, such as methionine. In addition, sesame also contains antioxidant substances such as vitamin E, sesamin, sesamolin, and sesamol.

[0003] At present, the main sesame processing products in China are sesame oil, sesame paste, peeled sesame, roasted sesame, etc. Among them, sesame paste is a flavor food with high nutritional value and is often used in cold dishes, hot dry noodles, hot pot dipping sauces, etc. to enhance the flavor. Consuming sesame paste can not only meet people's taste bud needs, but also after sesame is ground, the nutrients and active ingredients in sesame are more easily absorbed by the human body, exerting its nutritional and health care value.

[0004] At present, during the circulation process of sesame paste products in the market, there is a problem of poor stability, that is, floating oil will precipitate after sesame paste is placed for one month. The longer the time, the more floating oil. The precipitation of floating oil in sesame paste leads to the caking and solidification of the lower layer of sesame paste body, which has an adverse impact on the original flavor, spreadability, and sensory quality of the product, shortens the storage period and shelf life, affects the popularity and commercial value of sesame paste processing products in the market, and restricts the expansion of the scale of sesame paste products. How to make sesame paste maintain high stability and slow down or prevent the precipitation of floating oil in sesame paste is the key to solving the large-scale production of sesame paste. Some domestic scholars have studied to improve the product stability by adding natural antioxidants and emulsifiers. Although the stability of sesame paste has been improved to a certain extent, there are two problems. One is that for the added external additives, the product name cannot be marked as "pure sesame paste"; the other is that it affects the flavor of the product. Summary of the Invention

[0005] The purpose of the present invention is to add substances contained in sesame paste itself to sesame paste to improve the stability of sesame paste without affecting its purity and flavor.

[0006] The first aspect of the present invention provides a method for preparing a sesame extract. The method includes drying the sesame raw material residue obtained after degreasing treatment to obtain a solid component A, then performing alcohol extraction on the solid component A to obtain an alcohol-soluble component, and performing extraction on the alcohol-soluble component to obtain an aqueous layer extract B. The component A and the component B are compounded into an extract C in a mass ratio of 1:1 - 1:3 to obtain the sesame extract, wherein the volume concentration of the aqueous solution of alcohol is greater than or equal to 70%.

[0007] In one or more embodiments, the sesame raw material is selected from one or more of sesame seeds, sesame paste, sesame meal, and sesame cake.

[0008] In one or more embodiments, the degreasing includes obtaining a solid component by drying the residue obtained by leaching with an organic solvent such as n-hexane.

[0009] In one or more embodiments, the alcohol extraction uses an alcohol solution to extract the solid component obtained in the degreasing step to obtain an alcohol-soluble component.

[0010] In one or more embodiments, the alcohol is ethanol.

[0011] In one or more embodiments, the volume concentration of the alcohol in the aqueous solution of alcohol is 20% - 80%.

[0012] In one or more embodiments, the volume concentration of the alcohol in the aqueous solution of alcohol is 70 ± 10%, preferably 70 - 80%.

[0013] In one or more embodiments, the solid component is leached with an ethanol solution several times, preferably 2 - 3 times.

[0014] In one or more embodiments, the solid component is leached with an ethanol solution, wherein the ratio of material to liquid is 1:(3 - 5).

[0015] In one or more embodiments, the extraction includes using n-butanol to extract the alcohol-soluble component dissolved in water to obtain an n-butanol layer component.

[0016] In one or more embodiments, the method includes:

[0017] Degreasing: The sesame raw material is leached with n-hexane several times, the filtrates obtained each time are combined, and n-hexane is removed to obtain a solid component A;

[0018] Alcohol extraction: The solid component is leached with an ethanol solution with a volume concentration of 70 ± 10% several times, the filtrates obtained each time are combined, and the solvent is removed to obtain an alcohol-soluble component;

[0019] Extraction: Dissolve the alcohol-soluble components in water, and extract several times with a water-saturated n-butanol solution with a volume ratio of 1:(1-3). Combine the water layer extraction parts, and concentrate by vacuum rotary evaporation to obtain the water layer component B;

[0020] Compound: Mix component A and component B in a ratio of 1:(1-3), and incubate at 60-90 °C for more than 20 h to obtain component C.

[0021] In one or more embodiments, the number of times of extraction with n-hexane at room temperature is preferably 2-3 times.

[0022] In one or more embodiments, the degreasing includes extraction with n-hexane, and the material-liquid ratio is preferably 1:(2-3).

[0023] In one or more embodiments, extraction is carried out with n-hexane, and the extraction time is preferably 3-6 h.

[0024] In one or more embodiments, the volume ratio of the alcohol-soluble component to water is 1:(2-3), such as 1:2.

[0025] In one or more embodiments, the n-butanol is a water-saturated n-butanol solution, preferably a 1:(1-3) water-saturated n-butanol solution, and more preferably a 1:1 water-saturated n-butanol solution.

[0026] In one or more embodiments, the compounding is to mix component A and component B in a mass ratio of 1:(1-3), and incubate at 60-90 °C for more than 20 h to obtain component C.

[0027] In one or more embodiments, the incubation temperature is 70-90 °C, and more preferably, the temperature is 80 °C.

[0028] In one or more embodiments, the incubation time is 20-30 h, and more preferably, the incubation time is 24 h.

[0029] The second aspect of the present invention provides a sesame extract prepared by the method according to any one of the embodiments herein.

[0030] In one or more embodiments, the extract includes component A and component B, and the mixing ratio of component A to component B is 1:1:(1-3).

[0031] The third aspect of the present invention provides a method for improving the stability of sesame paste, the method including adding the sesame extract or its aqueous solution according to any one of the embodiments herein to the sauce for preparing sesame paste during the preparation of sesame paste, and the step of preparing sesame paste from the sauce added with the sesame paste extract; wherein, the amount of the added sesame extract is more than 1% of the weight of the sauce, preferably 1-3%, and more preferably 1-2%.

[0032] In one or more embodiments, the method further comprises the step of preparing a sesame extract as described in any of the embodiments herein.

[0033] The fourth aspect of the present invention provides a sesame paste, which contains more than 1%, preferably 1-3%, more preferably 1-2% of the aforementioned sesame extract based on the weight of the sesame paste.

[0034] The fifth aspect of the present invention provides the use of the method and / or the sesame extract as described in any of the embodiments herein in enhancing the stability of a sauce; preferably, the sauce is a sesame paste.

[0035] The sixth aspect of the present invention provides a food product, which contains the sesame extract or the sesame paste as described in any of the embodiments herein. Detailed Description

[0036] As used herein, terms such as "comprising", "including", "containing" and the like encompass the meanings of "consisting essentially of" and "consisting of". For example, when it is disclosed herein that "A comprises B and C", "A consists essentially of B and C" and "A consists of B and C" should be considered to have been disclosed herein.

[0037] As used herein, unless otherwise specified, percentages refer to mass (or weight) percentages and ratios refer to mass (or weight) ratios.

[0038] As used herein, the term "paste" refers to the part remaining after removing water from a conventional sauce. When calculating the content based on the mass or weight of the "paste", the mass or weight of the "paste" does not include the mass or weight of the concentrated sesame oil extract and water described herein; when calculating the content based on the mass or weight of the "sauce", the mass or weight of the "sauce" refers to the mass or weight of the entire sauce, that is, the mass or weight of water and the concentrated sesame oil extract is also included.

[0039] As used herein, when describing an embodiment or an example, it should be understood that it is not intended to limit the present invention to these embodiments or examples. On the contrary, all alternatives, modifications and equivalents of the methods and materials described in the present invention can be covered within the scope defined by the claims.

[0040] As used herein, for the sake of brevity of description, all possible combinations of all technical features in each embodiment or example are not described. Therefore, as long as there is no contradiction in the combination of these technical features, the technical features in each embodiment or example can be combined arbitrarily, and all possible combinations should be considered to be within the scope described in this specification.

[0041] After in-depth research, the inventors found that adding sesame extract to sesame paste can improve the stability of sesame paste, thus completing the present invention. In this article, the stability is characterized by the centrifugal floating oil rate, and the lower the centrifugal floating oil rate, the more stable the sesame paste is.

[0042] The sesame extract described in this article can be an extract of sesame raw materials with alcohol or an aqueous solution of alcohol.

[0043] The sesame extract described in this article is prepared by including the following steps: degreasing, extraction, extraction, and compounding.

[0044] The degreasing step of this application can be implemented by using degreasing methods well-known in the art. For example, sesame raw materials can be extracted with an organic solvent such as n-hexane, separating the oil part and the solid insoluble part that is insoluble in the used organic solvent from the components dissolved in the organic solvent such as n-hexane, obtaining the residue remaining after extraction with n-hexane, and after drying to remove the residual solvent, obtaining solid component A.

[0045] In this article, the sesame raw materials can be one or more of sesame seeds, sesame paste, sesame meal, and sesame cake. When using sesame seeds, to improve the extraction efficiency, it is preferred to crush the sesame seeds or make them into sesame paste and then extract with an organic solvent such as n-hexane. In some embodiments, this application uses pure sesame paste as the sesame raw material for organic solvent extraction. There are no special restrictions on the extraction time and number of times, which can be determined according to the amount of the used organic solvent, the amount of the sesame raw material, and the state of the sesame raw material (such as whether it is sesame seeds or sesame paste). For example, the sesame raw materials can be extracted with an organic solvent more than once, such as 2 to 4 times. The extraction time for each time can be greater than 1 h. In some embodiments, the extraction time for each time is 2 to 8 h, such as 2 to 5 h, preferably 3 - 6 h. The number of times of n-hexane room temperature extraction is preferably 2 - 3 times; during extraction, the mass of the used organic solvent can be 1 to 5 times that of the sesame raw material, such as 2 to 4 times. For those implementing multiple extractions, the filtrate parts obtained each time can be combined. The filtrate can be concentrated by, for example, vacuum rotary evaporation and other methods to further remove the residual organic solvent in the filtrate and obtain the solid components dissolved in this organic solvent. The residue remaining after extraction with n-hexane is dried to remove the residual solvent to obtain solid component A. Drying can be hot air drying. The drying temperature can be 50 - 80 °C, preferably 60 - 70 °C.

[0046] In some embodiments, the degreasing step includes the steps of extracting with an organic solvent (such as n-hexane) to obtain an organic solvent layer, and removing the organic solvent in the organic solvent layer to obtain solid component A. The extraction method can be as described above, and the method for removing the organic solvent can include steps such as vacuum rotary evaporation and drying described above.

[0047] The extraction step of the present invention can use the solid component obtained from the degreasing step by alcohol solution extraction to obtain an alcohol-soluble component. The alcohol used for extraction is preferably ethanol. An aqueous solution of alcohol can be used for extraction. Generally, the volume concentration of alcohol in the aqueous solution of alcohol is 20% - 80%, such as 70 ± 10%, preferably 70 - 80%. In some embodiments, an aqueous ethanol solution with a volume concentration of 75% is used for alcohol extraction (leaching). During alcohol extraction, the mass of the extraction solvent (such as the aqueous solution of the alcohol) used can be 1 - 5 times, such as 2 - 4 times, preferably 2 - 3 times the mass of the solid component. It should be understood that the length and number of leaching times are related to the amount of the product obtained by extraction. Therefore, those skilled in the art can select one or more leaching times and the time for each leaching according to the actual situation. For example, the time for each leaching can be more than 0.5 h, such as 1 - 8 h or 2 - 5 h. After the leaching is completed, the alcohol and water in the filtrate can be removed to obtain the alcohol-soluble component. The alcohol and water can be removed by, for example, vacuum rotary evaporation.

[0048] In the extraction step of the present invention, the alcohol-soluble component obtained from the extraction step can be first dissolved in water, and then extracted with n-butanol. The volume ratio of the alcohol-soluble component to water can be 1:(2 - 3), such as 1:2. The n-butanol is preferably a water-saturated n-butanol solution, preferably 1:(1 - 3), more preferably a 1:1 water-saturated n-butanol solution. When dissolving with n-butanol, ultrasound can be applied. It should be understood that the purpose of ultrasound is to promote dissolution. Therefore, those skilled in the art can select the conditions of ultrasound according to the actual situation. The exemplary ultrasound time can be 10 - 60 min, preferably 10 - 30 min. It should be understood that the number and length of extraction times are related to the amount of the product obtained by extraction. Therefore, those skilled in the art can select the appropriate number and time of extraction according to the actual situation. For example, the number of extractions can be 1 - 5 times, and the time for each extraction can be 10 min - 1 h. The n-butanol extraction part can be concentrated to obtain the n-butanol layer component. The water layer extraction parts are combined, evaporated and concentrated to obtain the water layer component B. Concentration can be carried out by, for example, rotary evaporation, and rotary evaporation can be carried out under vacuum conditions.

[0049] In the compounding step of the present invention: Component A and Component B are mixed in a mass ratio of 1:(1 - 3), and the ratio of Component A to Component B can be 1:1. Then the above composition is placed in a heatable device for incubation to obtain Component C. The temperature of the heatable device can be set to 60 - 90°C, preferably 70 - 90°C, more preferably, the temperature is set to 80°C; the incubation time is more than 20 h, preferably the incubation time is 20 - 30 h, and more preferably the time is 24 h. It should be understood that the purpose of heating is to glycosylate the proteins in Component A and Component B, and the temperature and the length of time are related to the yield of Component A and / or B. Therefore, those skilled in the art can select the heating temperature and time according to the actual situation. The heating method can be the form of oven heating incubation, or other heating methods such as a constant temperature incubator.

[0050] Thus, in some embodiments, the present invention provides a sesame extract, the components of which are substantially the same as those of the sesame extract prepared by the preparation method of the sesame extract described in any of the embodiments herein. "Substantially the same" as described herein means that among the components contained in the sesame extract, the components accounting for more than 10%, preferably more than 20%, more preferably more than 30% of the total weight of the sesame extract are completely the same.

[0051] An aqueous solution of the sesame extract can be formulated as needed. Generally, the sesame extract can be added to pure water and treated under ultrasonic conditions of 30 - 40°C and 50 - 200 Hz until completely dissolved, thereby preparing an aqueous solution of the sesame extract. There is no special limitation on the content of the sesame extract in the aqueous solution.

[0052] In some embodiments, the present invention provides the use of the sesame extract described in any of the embodiments herein in enhancing the stability of a sauce, or in the preparation of a sauce with improved stability; the sauce is preferably sesame paste.

[0053] In some embodiments, the present invention provides a sauce having improved stability. In the sauce with improved stability of the present invention, based on the mass of the sauce, the content of the sesame extract is ≥1%, and in some embodiments, the content of the sesame extract is 1% - 3%. In some embodiments, the content of the sesame extract is 1% - 2%.

[0054] In some embodiments, the centrifugal floating oil rate of the sauce is ≤17.3% or ≤16.53% or ≤15.58%; preferably, compared with a sauce having the same other components without the sesame extract, the centrifugal floating oil rate of the sauce is reduced by at least 7.18% or at least 11.41% or at least 16.51%. In some embodiments, the sauce is sesame paste.

[0055] In some embodiments, the present invention provides a method for preparing a sauce or a method for enhancing the stability of a sauce. During the process of preparing the sauce, the sesame extract or its aqueous solution described in any of the embodiments herein can be directly added to the semi-finished sauce or the finished sauce, and the sauce is prepared from the semi-finished sauce added with the sesame extract or its aqueous solution, and the sauce has enhanced stability. Alternatively, the sesame extract or its aqueous solution can be added to the prepared product sauce and stirred evenly to prepare a sauce with enhanced stability. The finished sauce or semi-finished sauce can be prepared by methods well known in the art. For example, when the sauce is sesame paste, the preparation method of sesame paste includes steps of screening sesame, washing with water, drying, and grinding. For other sauces, their preparation methods may not be similar to that of sesame paste, but the sesame extract or its aqueous solution described in any of the embodiments herein can also be added (usually added to the prepared semi-finished sauce) in appropriate preparation steps.

[0056] Therefore, in some embodiments, the method for preparing a sauce or the method for enhancing the stability of a sauce described herein further includes the step of preparing a semi-finished or finished sauce.

[0057] In some embodiments, the method for preparing a sauce or the method for enhancing the stability of a sauce described herein may further include the step of preparing the sesame extract or its aqueous solution by the method described in any of the previous embodiments.

[0058] In some embodiments, the present invention further provides a mixed sauce, which contains the above-mentioned sauce with enhanced stability.

[0059] The present invention further provides a food, which contains the above-mentioned sauce or is prepared from the above-mentioned sauce.

[0060] The present invention has the following beneficial effects:

[0061] The present invention adds the substances in the sesame paste itself to the sesame paste to improve the stability of the sesame paste, and the obtained sesame paste is not easy to float with oil and form lumps.

[0062] The present invention will be further described below by way of specific examples. It should be understood that these examples are merely illustrative and not intended to limit the scope of the present invention. The methods and reagents used in the examples are conventional methods and reagents in the art unless otherwise specified.

[0063] Materials

[0064] Sesame paste: Pure sesame paste retailed on the market;

[0065] Example 1

[0066] Degreasing: Weigh 200 g of commercially available sesame paste, and then extract it twice at room temperature with n-hexane (solid-liquid ratio 1:2), each extraction for 4 h. Combine the filtrates and concentrate by vacuum rotary evaporation to obtain the n-hexane solvent layer component. After the residue remaining after n-hexane extraction is dried with hot air at 65 °C to remove the residual solvent, solid component A is obtained.

[0067] Extraction: The solid component is extracted three times at room temperature with 75% ethanol (solid-liquid ratio 1:3), each extraction for 4 h. Combine the filtrates and concentrate by vacuum rotary evaporation to obtain the alcohol-soluble component; the residue remaining after ethanol extraction is dried with hot air at 65 °C to remove the residual solvent, and the alcohol-insoluble component is obtained.

[0068] Extraction: Dissolve the alcohol-soluble component in water (solid-liquid ratio 1:1), and then extract it three times with a 1:1 water-saturated n-butanol solution (ultrasonic for 10 min with 1:1 deionized water, take the upper layer with a separating funnel). Combine the water layer extraction part and concentrate by vacuum rotary evaporation to obtain water layer component B.

[0069] Configuration: Mix component A and component B in a ratio of 1:1, place them in an oven at 80 °C and incubate for 24 h to obtain component C, and add it to the sesame paste at a ratio of 1%.

[0070] Example 2

[0071] Degreasing: Follow the steps of Preparation Example 1.

[0072] Extraction: Follow the steps of Preparation Example 1.

[0073] Extraction: Follow the steps of Preparation Example 1.

[0074] Configuration: Mix component A and component B in a ratio of 1:2, place them in an oven at 80 °C and incubate for 24 h to obtain component C, and add it to the sesame paste at a ratio of 1%.

[0075] Example 3

[0076] Degreasing: Follow the steps of Preparation Example 1.

[0077] Extraction: Follow the steps of Preparation Example 1.

[0078] Extraction: Follow the steps of Preparation Example 1.

[0079] Configuration: Mix component A and component B in a ratio of 1:3, place them in an oven at 80 °C and incubate for 24 h to obtain component C, and add it to the sesame paste at a ratio of 1%.

[0080] Example 4

[0081] Degreasing: Follow the steps of Preparation Example 1.

[0082] Extraction: Follow the steps of Preparation Example 1.

[0083] Extraction: Follow the steps of Preparation Example 1.

[0084] Configuration: Mix Component A and Component B in a 1:1 ratio, place them in an oven at 80 °C for incubation for 28 h to obtain Component C, and add it to sesame paste at a ratio of 1%.

[0085] Example 5:

[0086] Degreasing: Follow the steps of Preparation Example 1.

[0087] Extraction: Follow the steps of Preparation Example 1.

[0088] Extraction: Follow the steps of Preparation Example 1.

[0089] Configuration: Mix Component A and Component B in a 1:1 ratio, place them in an oven at 80 °C for incubation for 24 h to obtain Component C, and add it to sesame paste at a ratio of 2%.

[0090] Example 6

[0091] Degreasing: Follow the steps of Preparation Example 1.

[0092] Extraction: Follow the steps of Preparation Example 1.

[0093] Extraction: Follow the steps of Preparation Example 1.

[0094] Configuration: Mix Component A and Component B in a 1:1 ratio, place them in an oven at 80 °C for incubation for 24 h to obtain Component C, and add it to sesame paste at a ratio of 3%.

[0095] Example 7

[0096] Degreasing: Weigh 200 g of commercially available sesame paste, then extract it twice with n - hexane at room temperature (material - to - liquid ratio 1:1), each extraction for 4 h. Combine the filtrates and concentrate by vacuum rotary evaporation to obtain the n - hexane solvent layer component. The residue remaining after n - hexane extraction is dried with hot air at 65 °C to remove the residual solvent, and solid Component A is obtained.

[0097] Extraction: Follow the steps of Preparation Example 1.

[0098] Extraction: Follow the steps of Preparation Example 1.

[0099] Configuration: Follow the steps of Preparation Example 1.

[0100] Example 8

[0101] Degreasing: Weigh 200 g of commercially available sesame paste, then extract it twice with n - hexane at room temperature (material - to - liquid ratio 1:3), each extraction for 4 h. Combine the filtrates and concentrate by vacuum rotary evaporation to obtain the n - hexane solvent layer component. The residue remaining after n - hexane extraction is dried with hot air at 65 °C to remove the residual solvent, and solid Component A is obtained.

[0102] Extraction: Follow the steps of Preparation Example 1.

[0103] Extraction: Follow the steps of Preparation Example 1.

[0104] Configuration: Follow the steps of Preparation Example 1.

[0105] Example 9

[0106] Degreasing: Weigh 200 g of commercially available sesame paste, and then extract it with n-hexane at room temperature 3 times (material-liquid ratio 1:2), each extraction for 4 h. Combine the filtrates and concentrate by vacuum rotary evaporation to obtain the n-hexane solvent layer component. The residue remaining after extraction with n-hexane is dried at 65 °C with hot air to remove the residual solvent, and solid component A is obtained.

[0107] Extraction: Follow the steps of Preparation Example 1.

[0108] Extraction: Follow the steps of Preparation Example 1.

[0109] Configuration: Follow the steps of Preparation Example 1.

[0110] Example 10

[0111] Degreasing: Weigh 200 g of commercially available sesame paste, and then extract it with n-hexane at room temperature 2 times (material-liquid ratio 1:2), each extraction for 5 h. Combine the filtrates and concentrate by vacuum rotary evaporation to obtain the n-hexane solvent layer component. The residue remaining after extraction with n-hexane is dried at 65 °C with hot air to remove the residual solvent, and solid component A is obtained.

[0112] Extraction: Follow the steps of Preparation Example 1.

[0113] Extraction: Follow the steps of Preparation Example 1.

[0114] Configuration: Follow the steps of Preparation Example 1.

[0115] Example 11

[0116] Degreasing: Weigh 200 g of commercially available sesame paste, and then extract it with n-hexane at room temperature 2 times (material-liquid ratio 1:2), each extraction for 6 h. Combine the filtrates and concentrate by vacuum rotary evaporation to obtain the n-hexane solvent layer component. The residue remaining after extraction with n-hexane is dried at 65 °C with hot air to remove the residual solvent, and solid component A is obtained.

[0117] Extraction: Follow the steps of Preparation Example 1.

[0118] Extraction: Follow the steps of Preparation Example 1.

[0119] Configuration: Follow the steps of Preparation Example 1.

[0120] Example 12

[0121] Degreasing: Follow the steps of Preparation Example 1.

[0122] Extraction: The solid component was extracted with 75% ethanol at room temperature 3 times (material-liquid ratio 1:4), each extraction for 4 h. The filtrates were combined and concentrated by vacuum rotary evaporation to obtain the alcohol-soluble component; the residue remaining after ethanol extraction was dried with hot air at 65 °C to remove the residual solvent, obtaining the alcohol-insoluble component.

[0123] Extraction: According to the steps of Preparation Example 1.

[0124] Formulation: According to the steps of Preparation Example 1.

[0125] Example 13

[0126] Degreasing: According to the steps of Preparation Example 1.

[0127] Extraction: The solid component was extracted with 75% ethanol at room temperature 4 times (material-liquid ratio 1:3), each extraction for 4 h. The filtrates were combined and concentrated by vacuum rotary evaporation to obtain the alcohol-soluble component; the residue remaining after ethanol extraction was dried with hot air at 65 °C to remove the residual solvent, obtaining the alcohol-insoluble component.

[0128] Extraction: According to the steps of Preparation Example 1.

[0129] Formulation: According to the steps of Preparation Example 1.

[0130] Example 14

[0131] Degreasing: According to the steps of Preparation Example 1.

[0132] Extraction: The solid component was extracted with 75% ethanol at room temperature 3 times (material-liquid ratio 1:5), each extraction for 3 h. The filtrates were combined and concentrated by vacuum rotary evaporation to obtain the alcohol-soluble component; the residue remaining after ethanol extraction was dried with hot air at 65 °C to remove the residual solvent, obtaining the alcohol-insoluble component.

[0133] Extraction: According to the steps of Preparation Example 1.

[0134] Formulation: According to the steps of Preparation Example 1.

[0135] Example 15

[0136] Degreasing: According to the steps of Preparation Example 1.

[0137] Extraction: The solid component was extracted with 70% ethanol at room temperature 3 times (material-liquid ratio 1:3), each extraction for 4 h. The filtrates were combined and concentrated by vacuum rotary evaporation to obtain the alcohol-soluble component; the residue remaining after ethanol extraction was dried with hot air at 65 °C to remove the residual solvent, obtaining the alcohol-insoluble component.

[0138] Extraction: According to the steps of Preparation Example 1.

[0139] Formulation: According to the steps of Preparation Example 1.

[0140] Example 16

[0141] Degreasing: According to the steps of Preparation Example 1

[0142] Extraction: The solid component was extracted with 80% ethanol at room temperature 3 times (material-liquid ratio 1:3), each extraction for 4 h, the filtrates were combined, and concentrated by vacuum rotary evaporation to obtain the alcohol-soluble component; the residue remaining after ethanol extraction was dried with hot air at 65 °C to remove the residual solvent to obtain the alcohol-insoluble component.

[0143] Extraction: According to the steps of Preparation Example 1.

[0144] Formulation: According to the steps of Preparation Example 1.

[0145] Example 17

[0146] Degreasing: According to the steps of Preparation Example 1

[0147] Extraction: According to the steps of Preparation Example 1

[0148] Extraction: The alcohol-soluble component was dissolved in water (material-liquid ratio 1:2), and then extracted 3 times with a 1:1 water-saturated n-butanol solution (1:1 deionized water ultrasonicated for 10 min, the upper layer was taken with a separatory funnel), the water layer extraction parts were combined, and concentrated by vacuum rotary evaporation to obtain the water layer component B.

[0149] Formulation: According to the steps of Preparation Example 1.

[0150] Example 18

[0151] Degreasing: According to the steps of Preparation Example 1.

[0152] Extraction: According to the steps of Preparation Example 1

[0153] Extraction: The alcohol-soluble component was dissolved in water (material-liquid ratio 1:3), and then extracted 3 times with a 1:1 water-saturated n-butanol solution (1:1 deionized water ultrasonicated for 10 min, the upper layer was taken with a separatory funnel), the water layer extraction parts were combined, and concentrated by vacuum rotary evaporation to obtain the water layer component B.

[0154] Formulation: According to the steps of Preparation Example 1.

[0155] Example 19

[0156] Degreasing: According to the steps of Preparation Example 1.

[0157] Extraction: According to the steps of Preparation Example 1.

[0158] Extraction: The alcohol-soluble component was dissolved in water (material-liquid ratio 1:3), and then extracted 3 times with a 1:2 water-saturated n-butanol solution (1:1 deionized water ultrasonicated for 10 min, the upper layer was taken with a separatory funnel), the water layer extraction parts were combined, and concentrated by vacuum rotary evaporation to obtain the water layer component B.

[0159] Configuration: Follow the steps of Preparation Example 1.

[0160] Example 20

[0161] Degreasing: Follow the steps of Preparation Example 1.

[0162] Extraction: Follow the steps of Preparation Example 1.

[0163] Liquid - liquid extraction: Dissolve the alcohol - soluble component in water (material - liquid ratio 1:3), then extract 3 times with a 1:3 water - saturated n - butanol solution (ultrasonic treatment with 1:1 deionized water for 10 min, take the upper layer with a separating funnel), combine the water - layer extraction part, and concentrate by vacuum rotary evaporation to obtain the water - layer component B.

[0164] Configuration: Follow the steps of Preparation Example 1.

[0165] Example 21

[0166] Degreasing: Follow the steps of Preparation Example 1.

[0167] Extraction: Follow the steps of Preparation Example 1.

[0168] Liquid - liquid extraction: Dissolve the alcohol - soluble component in water (material - liquid ratio 1:3), then extract 3 times with a 1:1 water - saturated n - butanol solution (ultrasonic treatment with 1:2 deionized water for 10 min, take the upper layer with a separating funnel), combine the water - layer extraction part, and concentrate by vacuum rotary evaporation to obtain the water - layer component B.

[0169] Configuration: Follow the steps of Preparation Example 1.

[0170] Example 22

[0171] Degreasing: Follow the steps of Preparation Example 1.

[0172] Extraction: Follow the steps of Preparation Example 1.

[0173] Liquid - liquid extraction: Dissolve the alcohol - soluble component in water (material - liquid ratio 1:3), then extract 3 times with a 1:1 water - saturated n - butanol solution (ultrasonic treatment with 1:3 deionized water for 10 min, take the upper layer with a separating funnel), combine the water - layer extraction part, and concentrate by vacuum rotary evaporation to obtain the water - layer component B.

[0174] Configuration: Follow the steps of Preparation Example 1.

[0175] Example 23

[0176] Degreasing: Follow the steps of Preparation Example 1.

[0177] Extraction: Follow the steps of Preparation Example 1.

[0178] Extraction: Dissolve the alcohol-soluble components in water (material-liquid ratio 1:3), then extract 3 times with a 1:1 water-saturated n-butanol solution (1:1 deionized water ultrasonicated for 20 min, take the upper layer with a separatory funnel), combine the water layer extraction part, and concentrate by vacuum rotary evaporation to obtain the water layer component B.

[0179] Configuration: Follow the steps of Preparation Example 1.

[0180] Example 24

[0181] Degreasing: Follow the steps of Preparation Example 1.

[0182] Extraction: Follow the steps of Preparation Example 1.

[0183] Extraction: Dissolve the alcohol-soluble components in water (material-liquid ratio 1:3), then extract 3 times with a 1:1 water-saturated n-butanol solution (1:1 deionized water ultrasonicated for 30 min, take the upper layer with a separatory funnel), combine the water layer extraction part, and concentrate by vacuum rotary evaporation to obtain the water layer component B.

[0184] Configuration: Follow the steps of Preparation Example 1.

[0185] Example 25

[0186] Degreasing: Follow the steps of Preparation Example 1.

[0187] Extraction: Follow the steps of Preparation Example 1.

[0188] Extraction: Dissolve the alcohol-soluble components in water (material-liquid ratio 1:3), then extract 5 times with a 1:1 water-saturated n-butanol solution (1:1 deionized water ultrasonicated for 20 min, take the upper layer with a separatory funnel), combine the water layer extraction part, and concentrate by vacuum rotary evaporation to obtain the water layer component B.

[0189] Configuration: Follow the steps of Preparation Example 1.

[0190] Example 26

[0191] Degreasing: Follow the steps of Preparation Example 1.

[0192] Extraction: Follow the steps of Preparation Example 1.

[0193] Extraction: Follow the steps of Preparation Example 1.

[0194] Configuration: Mix component A and component B in a 1:1 ratio, place them in an oven at 70 °C for incubation for 24 h to obtain component C, and add it to sesame paste at a ratio of 1%.

[0195] Example 27

[0196] Degreasing: Follow the steps of Preparation Example 1.

[0197] Extraction: Follow the steps of Preparation Example 1.

[0198] Extraction: Follow the steps of Preparation Example 1.

[0199] Configuration: Mix Component A and Component B in a 1:1 ratio, place them in an oven at 90 °C and incubate for 24 h to obtain Component C, and add it to sesame paste at a ratio of 1%.

[0200] Example 28

[0201] Degreasing: Follow the steps of Preparation Example 1.

[0202] Extraction: Follow the steps of Preparation Example 1.

[0203] Extraction: Follow the steps of Preparation Example 1.

[0204] Configuration: Mix Component A and Component B in a 1:1 ratio, place them in an oven at 80 °C and incubate for 20 h to obtain Component C, and add it to sesame paste at a ratio of 1%.

[0205] Example 29

[0206] Degreasing: Follow the steps of Preparation Example 1.

[0207] Extraction: Follow the steps of Preparation Example 1.

[0208] Extraction: Follow the steps of Preparation Example 1.

[0209] Configuration: Mix Component A and Component B in a 1:1 ratio, place them in an oven at 80 °C and incubate for 30 h to obtain Component C, and add it to sesame paste at a ratio of 1%.

[0210] Comparative Example 1

[0211] Degreasing: Follow the steps of Preparation Example 1.

[0212] Extraction: Follow the steps of Preparation Example 1.

[0213] Extraction: Follow the steps of Preparation Example 1.

[0214] Configuration: Mix Component A and Component B in a 2:1 ratio, place them in an oven at 80 °C and incubate for 24 h to obtain Component C, and add it to sesame paste at a ratio of 1%.

[0215] Comparative Example 2

[0216] Degreasing: Follow the steps of Preparation Example 1.

[0217] Extraction: Follow the steps of Preparation Example 1.

[0218] Extraction: Follow the steps of Preparation Example 1.

[0219] Configuration: Mix Component A and Component B in a 1:4 ratio, place them in an oven at 80 °C and incubate for 24 h to obtain Component C, and add it to sesame paste at a ratio of 1%.

[0220] Comparative Example 3

[0221] Degreasing: Follow the steps of Preparation Example 1.

[0222] Extraction: Follow the steps of Preparation Example 1.

[0223] Extraction: Follow the steps of Preparation Example 1.

[0224] Configuration: Mix Component A and Component B in a 1:1 ratio, place them in an oven at 80 °C and incubate for 8 h to obtain Component C, and add it to sesame paste at a ratio of 1%.

[0225] Comparative Example 4

[0226] Degreasing: Follow the steps of Preparation Example 1.

[0227] Extraction: Follow the steps of Preparation Example 1.

[0228] Extraction: Follow the steps of Preparation Example 1.

[0229] Configuration: Mix Component A and Component B in a 1:1 ratio, place them in an oven at 60 °C and incubate for 24 h to obtain Component C, and add it to sesame paste at a ratio of 1%.

[0230] Comparative Example 5

[0231] Degreasing: Follow the steps of Preparation Example 1.

[0232] Extraction: Follow the steps of Preparation Example 1.

[0233] Extraction: Follow the steps of Preparation Example 1.

[0234] Configuration: Mix Component A and Component B in a 1:1 ratio, place them in an oven at 100 °C and incubate for 24 h to obtain Component C, and add it to sesame paste at a ratio of 1%.

[0235] Comparative Example 6

[0236] Degreasing: Follow the steps of Preparation Example 1.

[0237] Extraction: Follow the steps of Preparation Example 1.

[0238] Extraction: Follow the steps of Preparation Example 1.

[0239] Configuration: Mix Component A and Component B in a 1:1 ratio, place them in an oven at 80 °C and incubate for 24 h to obtain Component C, and add it to sesame paste at a ratio of 0.5%.

[0240] Comparative Example 7

[0241] Degreasing: Follow the steps of Preparation Example 1.

[0242] Extraction: Follow the steps of Preparation Example 1.

[0243] Extraction: Follow the steps of Preparation Example 1.

[0244] Preparation: Mix Component A and Component B in a 1:1 ratio, place them in an oven at 80 °C for incubation for 24 h to obtain Component C, and add it to sesame paste at a ratio of 4%.

[0245] Comparative Example 8

[0246] Pure sesame paste, without any additives

[0247] Centrifugal floating oil rate

[0248] Weigh 10.00 g of sesame paste, place it in a 150 mL centrifuge tube, centrifuge at a rate of 8000 r / min for 5 min, weigh the upper floating oil, and record its weight as m1. Calculate the centrifugal floating oil rate using the following formula:

[0249] ES = (10.00 - m1 / 10.00) * 100%

[0250] Where m1 is the mass of the upper floating oil.

[0251] Table 1 Detection results of sesame paste

[0252]

[0253]

[0254]

[0255] Note: In Comparative Example 7, when 4% of the composition was added, the sesame paste was too viscous. However, the sesame paste required in the market should have a certain fluidity. Therefore, it is recommended that the addition ratio should not exceed 4%.

[0256] The results show that when Component A and Component B are mixed in a ratio of 1:3, 1:2, and 1:1, placed in an oven at 60 - 90 °C for incubation for more than 20 h to obtain Component C, and Component C is added to sesame paste at a ratio of 1% - 3%, the floating oil can be significantly improved, and the floating oil reduction rate (compared with pure sesame paste) is 7.18 - 16.5%.

[0257] The embodiments described above merely represent several implementation manners of the present invention. The description thereof is relatively specific and detailed, but it should not be construed as a limitation on the scope of the patent for the present invention. It should be noted that for those of ordinary skill in the art, without departing from the concept of the present invention, several variations and improvements can still be made, and these all fall within the protection scope of the present invention. Therefore, the protection scope of the patent for the present invention shall be subject to the appended claims. At the same time, all the documents mentioned in the present invention are cited in this application for reference, just as if each document is separately cited for reference.

Claims

1. A method for preparing a sesame extract, characterized in that, The method includes the following steps: (1) Obtaining Component A: The sesame raw material residue obtained after degreasing treatment is dried to obtain solid Component A. (2) Obtaining Component B: Solid Component A is subjected to alcohol extraction to obtain an alcohol-soluble component, the alcohol-soluble component is extracted, and an aqueous layer extract B is obtained. Among them, the volume concentration of the aqueous solution of alcohol is greater than or equal to 70%. (3) Compound formulation: The step of formulating Component A and Component B into Extract C at a mass ratio of 1:(1 - 3).

2. The preparation method according to claim 1, characterized in that, The method has one or more of the following characteristics: The sesame raw material is selected from one or more of sesame seeds, sesame paste, sesame meal, and sesame cake. The degreasing includes extraction with an organic solvent such as n-hexane, and the residue after extraction is dried to obtain solid Component A. The alcohol extraction uses an alcohol solution to extract the solid component obtained in the degreasing step to obtain an alcohol-soluble component; preferably, the alcohol is ethanol; preferably, the volume concentration of the alcohol in the aqueous solution of alcohol is 70 ± 10%, preferably 70% - 80%. The extraction includes using n-butanol to extract the alcohol-soluble component dissolved in water to obtain an n-butanol layer component; and the aqueous layer extraction part, and the aqueous layer extraction part is evaporated and concentrated, preferably by vacuum rotary evaporation, to obtain aqueous layer Component B; among them, preferably, the volume ratio of the alcohol-soluble component to water is 1:(2 - 3), such as 1:2; preferably, the n-butanol is a water-saturated n-butanol solution, preferably a 1:(1 - 3) water-saturated n-butanol solution.

3. The preparation method according to claim 1 or 2, characterized in that, The method includes: Degreasing: Extract the sesame raw material with n-hexane several times, combine the filtrates obtained each time, remove the n-hexane, and obtain solid Component A. Alcohol extraction: Extract the solid component with an ethanol solution with a volume concentration of 70 ± 10% several times, combine the filtrates obtained each time, remove the solvent, and obtain an alcohol-soluble component. Extraction: Dissolve the alcohol-soluble component in water, extract it with a 1:(1 - 3) water-saturated n-butanol solution several times, combine the aqueous layer extraction parts, and evaporate and concentrate to obtain aqueous layer Component B. Compound formulation: Mix Component A and Component B at a mass ratio of 1:(1 - 3), incubate at 60 - 90°C for more than 20 h to obtain Component C.

4. The preparation method according to claim 3, characterized in that, The method satisfies one or more of the following conditions: The number of times of n-hexane extraction at room temperature in the degreasing step is 2 - 3 times; and / or, The mass ratio of the material to the liquid in the degreasing step is preferably 1:(1 - 3); and / or, The extraction time in the degreasing step is preferably 3 - 6 h; and / or, The mass ratio of the material to the liquid in the alcohol extraction step is 1:(3 - 5); and / or, The heating temperature in the compound formulation step is 70 - 90°C, preferably, the temperature is 80°C; and / or, The incubation time in the compound formulation step is 20 - 30 h, preferably, the incubation time is 24 h.

5. Sesame extract prepared by the method according to any one of claims 1 to 4; characterized in that, The extract includes Component A and Component B, and the mixing mass ratio of Component A and Component B is 1:1:(1 - 3).

6. A method for improving the stability of sesame paste, characterized in that, The method includes the step of adding the sesame extract or its aqueous solution according to claim 5 to the sauce used for preparing sesame paste during the preparation of sesame paste; among them, the amount of the added sesame extract is more than 1% of the total weight of the sauce, preferably 1 - 3%, more preferably 1 - 2%.

7. The method according to claim 6, wherein The method further includes the step of preparing the sesame extract by the method according to any one of claims 1 - 4.

8. A sauce, characterized in that, The sauce contains the sesame extract as described in claim 5 in an amount of 1% or more, preferably 1-3%, more preferably 1-2% by weight thereof; Preferably, the centrifugal floating oil rate of the sauce is ≤17.3%; Preferably, the sauce is sesame paste.

9. The application of the method as described in any one of claims 1-4 and / or the sesame extract as described in claim 5 in enhancing the stability of the sauce; preferably, the sauce is sesame paste.

10. A food product, characterized in that, The food contains the sesame extract as described in claim 5 or the sauce as described in claim 8.

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