Soothing composition, soothing product, application of soothing composition and soothing product and cosmetics

Abstract

The invention belongs to the field of daily chemicals, and discloses a soothing composition which is obtained by taking inonotus obliquus, radix saposhnikoviae and rye seeds as raw materials through water extraction and then extraction. The weight ratio of the inonotus obliquus to the radix saposhnikoviae to the rye seeds is (1-3): (5-15): (2-5). The composition takes inonotus obliquus, radix saposhnikoviae and rye seeds as raw materials, and a product with a better soothing effect can be obtained through water extraction; the further research of the invention finds that after the commercially available rye seed extract is additionally added into the composition, the soothing effect of the composition is more obvious. Meanwhile, the invention further discloses the soothing product, application of the soothing product and cosmetics.

Description

Technical Field

[0001] The present invention relates to the daily chemical field, and in particular to a soothing composition, a soothing product, its application and cosmetics. Background Art

[0002] Soothing skin care products mainly have the effects of relieving flushing and sensitivity, repairing the barrier, moisturizing and calming, anti-inflammatory and antibacterial, etc. Its core function is to help sensitive or damaged skin return to a stable state.

[0003] A patent application with the publication number CN113398050A and the theme of a mite-removing and oil-control shower gel discloses that the extract of Inonotus obliquus has strong oil-control, antioxidant, anti-inflammatory, and anti-allergy effects, and its anti-allergy traditional Chinese medicine composition is composed of the extracts of Lithospermum erythrorhizon, Saposhnikovia divaricata, Polygonatum odoratum, Angelica sinensis, and Glycyrrhiza glabra.

[0004] In the field of traditional Chinese medicine, Saposhnikovia divaricata has functions such as reducing fever, relieving pain, and regulating the immune system. Modern research has found that it can reduce fever, relieve headache and joint pain, reduce skin redness caused by allergies, enhance the body's resistance, and also assist in improving the problem of blood viscosity and inhibiting the growth of abnormal cells.

[0005] In the prior research of this application, it has been proved that the combination of Saposhnikovia divaricata and Inonotus obliquus has a good soothing effect. However, the soothing effect of these two substances is more obvious in terms of inhibiting histamine in IgE-induced RBL-2H3 cells, and the effect in terms of inhibiting hyaluronidase needs to be improved. Hyaluronidase has a strong correlation with the release of histamine by mast cells, etc. This shows that the improvement of the soothing effect is determined by multiple pathways. Summary of the Invention

[0006] The purpose of the present invention is to provide a soothing composition, which uses Inonotus obliquus, Saposhnikovia divaricata root, and rye seeds as raw materials and can obtain a product with better soothing effect through water extraction; in the further research of the present invention, it is found that when a commercially available rye seed extract is additionally added to the above composition, its soothing effect is more obvious.

[0007] At the same time, the present invention also discloses the application of the composition and the product, as well as cosmetics.

[0008] To achieve the above purpose, the present application discloses: A soothing composition, which is obtained by water extraction using Inonotus obliquus, Saposhnikovia divaricata root, and rye seeds as raw materials; The weight ratio of the Inonotus obliquus, Saposhnikovia divaricata root, and rye seeds is 1-3:5-15:2-5.

[0009] The characteristics of each raw material in the soothing composition of the present invention are: Inonotus obliquus: The active ingredients in Inonotus obliquus (such as triterpenoids) can inhibit the release of inflammatory factors and reduce inflammatory reactions such as tissue redness and swelling; Windproof: Reduce skin redness and swelling caused by allergies and enhance body resistance; Rye seeds: Extracts containing arabinoxylan can be extracted by water extraction. Arabinoxylan is mentioned in some cosmetic raw materials to have anti-aging functions. In the research of the present invention, it was found that when it was used alone, it did not have obvious soothing ability. When it was used in combination with siler and birch mushroom, the soothing ability of the formula was significantly enhanced. This result may be related to the antioxidant function of arabinoxylan, which can relieve inflammatory responses through multiple mechanisms.

[0010] The common mechanism of action of the above three is: Existing literature reports that inonotus birchii contains abundant inonotus alcohol, which is a type of polyphenol active substance that can inhibit IKKβ phosphorylation, block NF-κB nuclear translocation, and reduce TNF-α and IL-6 release, while chromone in the root of saposhnikovia inhibits IκBα degradation and enhances the NF-κB inhibitory effect. In the present invention, the experimental results can be used to infer that the mechanism of action of the two is: through the joint action of the two, NF-κB activation is double blocked and the expression of inflammatory factors is reduced. The saposhnikovia extract can inhibit the degranulation of IgE-sensitized RBL-2H3 cells and reduce the level of inflammatory mediators. At the same time, the triterpenoid compounds in inonotus birchii can also activate Nrf2, promote the expression of HO-1 and SOD, and remove ROS. The phenolic acids in rye seeds can directly neutralize free radicals, thereby synergistically enhancing the antioxidant effect.

[0011] In the soothing composition, the water extraction process is: crushing the inonotus birchii, the radix siler and the rye seeds, adding water, extracting, concentrating and precipitating with alcohol, and concentrating the filtrate after filtering.

[0012] In the above-mentioned soothing composition, the water extraction process is: crush the raw materials and pass them through a 40-mesh sieve. The raw materials are inonotus birchii, silanol root, and rye seeds. Add water 10 times the volume of the crushed raw materials, stir and extract at 70-90°C, centrifuge to remove the residue, concentrate the filtrate, precipitate with alcohol, remove ethanol, and then add polyols, preservatives and water or other fermentation product filtrate.

[0013] In the above soothing composition, taking the total weight of the soothing composition as 100%, the weight of the raw materials is equivalent to 10-20% of the total weight of the soothing composition.

[0014] Meanwhile, the present invention also discloses a soothing product, comprising the soothing composition as described in any one of the above and rye seed extract; the rye seed extract accounts for 0.05 - 0.2 wt% of the total weight of the soothing composition; the rye seed extract is produced by French company Silab, and the content of the active ingredient in the rye seed extract is 5.50 wt%.

[0015] In the further research of the present invention, it is found that adding rye seed extract additionally can significantly improve the soothing ability of the product. The possible reason is that this raw material consists of arabinoxylan derived from rye seeds. By binding to TLR2 / 4 receptors, it blocks the downstream MyD88 signal transduction, inhibits the activation of the IKK complex, thereby reducing the degradation of IκBα and the nuclear translocation of NF-κB. It reduces the expression of pro-inflammatory factors, and at the same time can inhibit the phosphorylation of ERK and JNK, and reduce the generation of COX-2 and PGE2, thus alleviating the inflammatory response. By additionally supplementing arabinoxylan from rye seeds in the product, the problem of polysaccharide loss during the purification process of the product is solved, the content index of active substances is improved, making it more effective in inhibiting the expression of inflammatory factors, and thus achieving the soothing effect.

[0016] In addition, the present invention also discloses the use of the soothing composition as described in any one of the above or the soothing product as described above in the preparation of cosmetics.

[0017] Finally, the present invention also discloses a cosmetic, containing 0.1 - 30 wt% of the soothing composition as described in any one of the above or the soothing product as described above.

[0018] This application has at least the following beneficial effects: The composition of the present invention uses Inonotus obliquus, Saposhnikovia divaricata roots, and rye seeds as raw materials. Through water extraction, a product with better soothing effect can be obtained; in the further research of the present invention, it is found that when a commercially available rye seed extract is additionally added to the above composition, its soothing effect is more obvious. Description of the Drawings

[0019] Figure 1 Photograph of the chorioallantoic membrane test of chicken embryos for Sample 7. Detailed Embodiments

[0020] The present invention will be described clearly and completely in conjunction with the embodiments of the present invention. In the description of the present invention, it should be noted that for those conditions not specified in the embodiments, they are carried out according to conventional conditions or conditions recommended by the manufacturer. For those reagents or instruments without indicating the manufacturer, they are all conventional products that can be obtained through commercial purchase. Unless otherwise specified, all parts used in the embodiments of the present invention are in weight parts.

[0021] Source of Raw Materials The Inonotus obliquus, Radix Saposhnikoviae, and rye seeds were purchased from a traditional Chinese medicine store. The moisture content of the Inonotus obliquus was less than 13%, the moisture content of the Radix Saposhnikoviae was less than 10%, and the moisture content of the rye seeds was less than 8%.

[0022] The supplier of the rye seed extract is Silab, France. The active ingredient is the extract of rye (SECALE CEREALE) seeds, and its active ingredient content is 5.50 wt%.

[0023] Part 1: Preparation of the composition The Inonotus obliquus, Radix Saposhnikoviae, and rye seeds were ground. The total weight of the Inonotus obliquus, Radix Saposhnikoviae, and rye seeds was 15 g, and their ratio refers to Table 1. 10 times the volume of water was added, and the mixture was stirred and extracted at 80 °C for 3 h. The residue was removed by centrifugation, and the filtrate was concentrated to 1 / 3 of the original extraction volume at 65 °C. 95% ethanol equal in volume to the concentrated solution was added for ethanol precipitation. The ethanol-precipitated solution was centrifuged and concentrated to a non-alcohol state. Polyol and water were added to a total weight of 100 g, and the polyol accounted for 30% of the total weight of the added polyol and water. The ultrafiltrate was obtained by ultrafiltration. Preservatives (0.05% octanoyl hydroxamic acid, 0.08% ethylhexyl glycerin, 0.6% 1,2-hexanediol) were added to the ultrafiltrate, and the finished product was obtained by sterilization.

[0024] The formula table can refer to Table 1; Table 1 Formula table (unit: weight ratio) Inonotus obliquus Ledebouriella seseloides root Rye seed Sample 1 1 15 2 Sample 2 1.5 10 3.5 Sample 3 3 5 5 Sample 4 2 12 4 Sample 5 2.5 8 3 Control sample 1 1.5 10 0 Control sample 2 1.5 0 3.5 Control sample 3 0 10 3.5 Control sample 4 0 0 3.5

[0025] Part 2: Preparation of the soothing product During the addition of preservatives to the soothing composition in the first part above, rye seed extract (the dosage refers to Table 2) was added simultaneously, and the finished product was obtained by sterilization.

[0026] Table 2 Formula table

[0027] Part 3: Performance test

[0028] 3.1 Inflammatory factor IL-8 inhibition test Macrophages can be used as a cell model to study the inhibition of the content of inflammatory factor IL-8 by cosmetics. By stimulating with bacterial lipopolysaccharide (LPS), the content of inflammatory factor IL-8 in the negative control and the test sample group was measured to evaluate whether the test sample has an effect in inhibiting the content of inflammatory factor IL-8. The content of IL-8 was measured by the enzyme-linked immunosorbent assay (ELISA).

[0029] In this test, referring to the laboratory method, the results of the determination of the content of cell IL-8 inflammatory factor of the test sample and the negative control were compared. If the content of cell IL-8 inflammatory factor of the test sample was lower than that of the negative control, it could be considered that the test sample had a certain soothing effect.

[0030] This test method is an in vitro method, which provides a method for detecting the content of macrophage IL-8 inflammatory factor and can be used as one of the evidence supports for the soothing efficacy claim of cosmetic raw materials and finished products.

[0031] Test Materials and Methods Instrumentation: RT-6100 microplate reader; Cells used in the experiment: Macrophages (RAW264.7 cells); Test method: (1) Treatment of test samples Sample group: Dilute the sample with pure water to a 4% solution, then filter it through a 0.22 μm filter, and collect the filtrate as the sample mother liquor; Negative control: Basal culture medium (cell viability test); Basal culture medium containing LPS (determination of IL-8 inflammatory factor content).

[0032] (2) Test operation steps Cell viability test: Seed RAW264.7 cells in a 96-well plate. After 24 h, aspirate the culture medium, add the basal culture medium containing test samples at different concentrations. After 24 h, detect OD490nm by the MTT method, and calculate the effect of the test samples on the viability of RAW264.7 cells.

[0033] Determination of IL-8 inflammatory factor content: Seed RAW264.7 cells in a 96-well plate. After culturing for 24 h, replace the medium with the basal culture medium containing test samples at different concentrations. After 24 h, collect the cell supernatant and centrifuge. Aspirate the supernatant and use an ELISA kit to determine the IL-8 content in the cell culture supernatant, and obtain the effect of the test samples on the IL-8 content of macrophages.

[0034] Calculation formula .

[0035] Refer to Table 3 for test results; 3.2 Histamine inhibition rate test

[0036] RBL-2H3 cells can be used as a cell model for studying the inhibition of histamine content in cosmetics. Induce RBL-2H3 cells with IgE, measure the histamine content of the negative control and the test sample group, and evaluate whether the test sample has efficacy in inhibiting histamine content. The histamine content is measured by the enzyme-linked immunosorbent assay (ELISA).

[0037] This test refers to the laboratory method and compares the results of the determination of the histamine content in cells between the test sample and the negative control. If the histamine content in the cells of the test sample is lower than that of the negative control, it can be considered that the test sample has a certain soothing effect.

[0038] This test method is an in vitro method, which provides a method for detecting the histamine content of RBL-2H3 cells and can be used as one of the evidence supports for the claim of the soothing effect of cosmetic raw materials and finished products.

[0039] Test Materials and Methods Instrumentation: RT-6100 microplate reader; Cells used in the experiment: Rat basophilic leukemia cells (RBL-2H3 cells); Test method: (1) Treatment of test samples Sample group: Dilute the sample with pure water to a solution with a volume fraction of 4%, and then filter it through a 0.22 μm filter. Collect the filtrate as the sample mother liquor; Negative control: Basal culture medium (cell viability test); Basal culture medium containing inducer (histamine content determination).

[0040] (2) Test operation steps Cell viability test: Seed RBL-2H3 cells in a 96-well plate. After 24 h, aspirate the culture medium, add basal medium containing different concentrations of the test sample. After 24 h, detect OD490nm by the MTT method, and calculate the effect of the test sample on the viability of RBL-2H3 cells.

[0041] Histamine content determination: Seed RBL-2H3 cells in a 96-well plate. After culturing for 24 h, replace it with basal medium containing different concentrations of the test sample. After 24 h, collect the cell supernatant and centrifuge. Aspirate the supernatant and use an ELISA kit to determine the histamine content in the cell culture supernatant, and obtain the effect of the test sample on the histamine content of RBL-2H3 cells.

[0042] (3) Calculation formula .

[0043] Refer to Table 3 for the test results; 3.3 Hyaluronidase inhibition rate test Hyaluronidase is a specific lyase of hyaluronic acid and is a participant in allergic reactions, which has a strong correlation with the release of histamine by mast cells. Whether the test sample has a soothing effect can be judged by the hyaluronidase inhibition rate. The higher the hyaluronidase inhibition rate, the stronger the soothing effect of the substance, and vice versa.

[0044] In this test, the hyaluronidase inhibition rate test results of the test sample were compared with those of the negative control. If the inhibition rate of the test sample was higher than that of the negative control, it could be considered that the test sample had a soothing effect.

[0045] This test method is an in vitro method and is applicable to evaluating cosmetics that claim to achieve a soothing effect by inhibiting hyaluronidase.

[0046] Test materials and methods Instrumentation: BSA224S analytical balance, L6s ultraviolet spectrophotometer; Reagents: BR-grade hyaluronidase, BR-grade sodium hyaluronate.

[0047] Test method: (1) Treatment of control substances and test samples Sample group: Dilute with pure water to a sample concentration of 5%; Positive control substance (dipotassium glycyrrhizinate, purity ≥ 98%): Dilute with water to a positive control substance concentration of 3%; Negative control substance: Pure water.

[0048] (2) Test operation steps Set up a sample group, a sample background group, a solvent group, and a solvent background group. Three parallels need to be set up for each group. Add different reagent solutions to the four groups respectively, shake well, place at room temperature for 30 min for color development, and measure the absorbance value at a wavelength of 528 nm with an ultraviolet spectrophotometer.

[0049] (3) Calculation formula ; Where: A — is the absorbance of the reaction solution without the sample and without the enzyme; B — is the absorbance of the reaction solution without the sample and with the enzyme; C — is the absorbance of the reaction solution containing the sample and the enzyme; D — is the reaction solution containing the sample and without the enzyme.

[0050] Refer to Table 3 for the test results; Table 3 Results table Group Inflammatory factor inhibition rate / % Histamine inhibition rate / % Hyaluronidase inhibition rate / % Sample 1 18.77 23.52 42.35 Sample 2 15.83 24.89 44.57 Sample 3 16.95 22.17 46.78 Sample 4 14.17 26.89 40.35 Sample 5 13.03 21.56 39.91 Sample 6 20.33 31.48 61.64 Sample 7 25.85 34.86 65.41 Sample 8 28.92 30.59 61.20 Control sample 1 11.62 15.19 32.15 Control sample 2 10.25 11.74 57.87 Control sample 3 8.37 9.80 14.19 Control sample 4 3.09 4.22 5.76 Negative control / / -0.53 Positive control / / 68.34

[0051] Result analysis: 1. By comparing Comparative Sample 1 to Comparative Sample 3, it can be seen that Inonotus obliquus is the main factor for improving the hyaluronidase inhibition rate performance, and no obvious differences can be observed in the other two performances. By comparing Comparative Sample 4, it can be seen that after single extraction of rye seeds, no obvious effect was shown.

[0052] Among Samples 1 to 5, the contribution of the dosage of Inonotus obliquus to the improvement of hyaluronidase inhibition rate was not reflected, but the performance of Samples 1 to 5 in the above three tests was generally better than that of Comparative Samples 1 to 4; Among Samples 1 to 5, Sample 1 had the best inflammatory factor inhibition rate, Sample 4 had the best histamine inhibition rate, Sample 1 had the highest content of Saposhnikovia divaricata roots, and Sample 4 had the highest content of rye seeds. Combining the results of Comparative Samples 1 to 4, it can be roughly inferred that Saposhnikovia divaricata roots and rye seeds play relatively important roles in the inhibition rate of inflammatory factors, and rye seeds play a relatively important role in the inhibition rate of histamine; however, it should be noted that, in combination with the data of Comparative Example 4, the histamine inhibition performance of rye seeds used alone is poor, and it is relatively necessary to use rye seeds and Inonotus obliquus simultaneously. In addition, the results of Comparative Sample 1 suggest that the combination of Inonotus obliquus and Saposhnikovia divaricata roots also has good histamine inhibition effects. From Samples 1 and 2, when Inonotus obliquus and Saposhnikovia divaricata roots are relatively more, the relatively higher histamine inhibition ability can also reflect the histamine inhibition ability of the two from one side. Since the gap between various samples is not large, the above conclusions do not rule out the existence of detection errors.

[0053] The above results show that when water extraction is carried out within the formula range of the present invention, the performance of the obtained product is relatively balanced and excellent.

[0054] As can be seen from Samples 6 to 8, after adding rye seeds, the soothing performance is further enhanced; the possible reasons are that, on the one hand, there are material-level differences between commercially available products and self-extracted products, and on the other hand, by additionally adding rye seed arabinoxylan to the product, the problem of polysaccharide loss during the purification process of the product is solved, the active substance content index is improved, so that it can more effectively inhibit the expression of inflammatory factors, thereby achieving the soothing effect.

[0055] The allantoic membrane test of chicken embryos was carried out on Sample 7 of the present invention, and the test results can be seen in Table 4 and Figure 1 ; Table 4 Test Results Serial number Sample name Sample treatment method Test result (ES) 1 Negative control (9% normal saline) / 0.00 2 Positive control (1% SDS) / 18.00 3 Schiff base Dilute the sample into a 2% aqueous solution 2.67

[0056] Application Example A soothing product is prepared according to the formula table in Table 5, and the preparation method is as follows: Step 1: Pre-dissolve Phase B and stir to mix evenly; pre-mix and stir Phase C evenly for standby.

[0057] Step 2: Add the pure water in Phase A to the pot, start homogenization, and sequentially add the remaining raw materials in Phase A under the homogenization state, and homogenize at medium speed for 30 - 60 s. Stir and heat to 80 - 85 °C, and homogenize at medium speed for 1 - 2 minutes until there are no gel particles. After keeping warm for 30 min, stir and cool down.

[0058] Step 3: Cool down to 50°C, and sequentially add the pre-prepared Phase B and Phase C, and stir evenly.

[0059] Step 4: Cool down to 45°C, add Phase D, and stir evenly.

[0060] Table 5 Basic Soothing Product Formulation Table

Claims

1. A soothing composition, characterized in that, Using inonotus birchii, parsnip root and rye seeds as raw materials, it is obtained by water extraction; The weight ratio of the birch inonotus, the radix siler and the rye seeds is 1-3:5-15:2-5.

2. The soothing composition according to claim 1, wherein The water extraction process comprises the following steps: crushing the inonotus birchii, the radix siler and the rye seeds, adding water, extracting, concentrating and precipitating with alcohol, and concentrating the filtrate after filtering.

3. The soothing composition according to claim 2, wherein The water extraction process is as follows: the raw materials are crushed and passed through a 40-mesh sieve, the raw materials are inonotus birchii, siler roots, and rye seeds, water is added in an amount 10 times the volume of the crushed raw materials, and the extraction is stirred and centrifuged at 70 to 90° C. to remove the filter residue, the filtrate is concentrated, alcohol precipitated, and ethanol is removed, and then polyols, preservatives, and water or other fermentation product filtrate are added.

4. The soothing composition according to claim 3, wherein Taking the total weight of the soothing composition as 100%, the weight of the raw materials is equivalent to 10-20% of the total weight of the soothing composition.

5. A soothing product, characterized in that, It comprises the soothing composition as claimed in any one of claims 1 to 4 and a rye seed extract; the rye seed extract is equivalent to 0.05-0.2wt% of the total weight of the soothing composition; the rye seed extract is produced by Silab, France, and the active ingredient content in the rye seed extract is 5.50wt%.

6. Use of the soothing composition according to any one of claims 1 to 4 or the soothing product according to claim 5 in preparing cosmetics.

7. A cosmetic, characterized in that, Contains 0.1 to 30 wt % of the soothing composition according to any one of claims 1 to 4 or the soothing product according to claim 5.

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