Hydroponic symbiotic microbial system and hydroponic symbiotic method

Abstract

The invention provides a hydroponic symbiotic microbial system and a hydroponic symbiotic method, and relates to the technical field of microbiology. The hydroponic symbiotic microbial system comprises an arbuscular mycorrhizal fungus community, broom cladosporium, aspergillus, pseudomonas and blastomonas. According to the method, a microbial structure in a natural environment is simulated by constructing diversified microbial communities, so that the synergistic effect of the microbial communities is fully exerted; through interaction of modes of co-decomposing organic matters, promoting nutrient absorption, promoting biological control, enhancing plant stress resistance and the like, the stability of an ecological system is co-maintained, and the growth of plants is promoted.

Description

Technical Field

[0001] The present invention relates to the technical field of microbiology, and in particular, to a microbial system for hydroponic symbiosis and a hydroponic symbiosis method. Background Art

[0002] Existing hydroponic technologies can achieve a clean environment, controllable nutrients, space saving, and increased yield per unit area. In vegetable cultivation, they can also shorten the growth cycle, break the influence of natural phenology, and achieve precise regulation of growth factors. In scientific research, with the in-depth study of mechanisms, the requirements for externally added substances in cultivation experiments are precise. The adsorption effect of solid particles makes it difficult to estimate the exact concentration of root exposure. Moreover, in root-related research, it is difficult to elute the solid matrix from the roots, which restricts the observation of the root growth process. Therefore, hydroponics shows great advantages. In addition, in a large number of stress studies, heavy metals and the like are used as stress factors. The soil after the experiment poses a potential threat, while the treatment process of the waste liquid is relatively complete. And now with the prevalence of intelligent devices, it is more convenient to combine hydroponics with them to achieve refined and data-based management of planting. Hydroponics is highly regarded in the fields of modern agriculture and horticulture.

[0003] Arbuscular Mycorrhizal (AM) fungi are one of the most widely distributed microbial groups in the soil, and can form symbiotic structures with 80% of vascular plants and play physiological and ecological functions. Generally speaking, AM fungi can enhance the host's ability to cope with stresses such as salinity, heavy metals, and pests and diseases through various ways. However, because the arbuscular mycorrhizal fungal community is commonly used in soil cultivation, its root-soil mixed form is difficult to apply to the hydroponic system, which restricts the effective utilization of the biological functions of AM fungi in traditional hydroponic technologies.

[0004] In addition, existing hydroponic microbial application studies mostly focus on single strains or limited types of microorganisms, making it difficult to fully exert the synergistic effects of the microbial community. This simplified microbial system cannot fully simulate the complex microbial network in the natural ecosystem, which may lead to a decline in the adaptability of plants when facing complex environments. At the same time, single or limited types of microorganisms may also increase the risk of invasion by pathogenic microorganisms, affecting the stability of the hydroponic system and the healthy growth of plants. Summary of the Invention

[0005] The problem solved by the present invention is how to solve the problems that it is difficult for hydroponic technologies to utilize the arbuscular mycorrhizal fungal community and it is difficult to fully exert the synergistic effects of the microbial community in hydroponics.

[0006] To solve the above problems, the present invention provides a microbial system for hydroponic symbiosis and a hydroponic symbiosis method.

[0007] In a first aspect, the present invention provides a hydroponic symbiotic microbial system, comprising: an arbuscular mycorrhizal fungal community, Scytalidium, Aspergillus, Pseudomonas, and Gemmatimonas.

[0008] Optionally, the arbuscular mycorrhizal fungal community comprises one or more of Glomus, Acaulospora, Rhizophagus, Septoglomus, Claroideoglomus, Diversispora, Ambispora, Paraglomus, Scutellospora, Pacispora, Funneliformis, Claroideoglomus etunicatum, and Gigaspora.

[0009] Optionally, Glomus comprises one or more of Glomus reticulatum, Glomus bullatum, Glomus excavatum, Glomus microaggregatum, Glomus melanosporum, Glomus fasciculatum, Glomus convolutum, Glomus macrocarpum, Glomus halonatum, Glomus multicaule, Glomus hyderabadensis, Glomus macrocarpum, Glomus minimum, and Glomus longisporum; Acaulospora comprises one or more of Acaulospora colombiana, Acaulospora tuberculata, Acaulospora koskei, Acaulospora foveata, Acaulospora denticulata, Acaulospora gerdensis, Acaulospora morrowiae, Acaulospora bireticulata, and Acaulospora excavata; Rhizophagus comprises one or more of Rhizophagus intraradices and Rhizophagus clarus; Septoglomus comprises one or more of Septoglomus arenarium and Septoglomus constrictum; Claroideoglomus comprises one or more of Claroideoglomus etunicatum, Claroideoglomus claroideum, and Claroideoglomus lamellosum; Diversispora comprises Diversispora epigaea; Ambispora comprises one or more of Ambispora leptoticha, Ambispora fennica, and Ambispora koskei; Paraglomus comprises one or more of Paraglomus occultum and Paraglomus brasilianum; Scutellospora comprises one or more of Scutellospora reticulata and Scutellospora nigra; Pacispora comprises one or more of Pacispora sasae and Pacispora boliviana; Funneliformis comprises one or more of Funneliformis geosporum and Funneliformis coronatum; Claroideoglomus etunicatum comprises Claroideoglomus mosseae; Gigaspora comprises Gigaspora decipiens; the arbuscular mycorrhizal fungal community comprises at least three species of Glomeromycota.

[0010] In a second aspect, the present invention provides a hydroponic symbiotic method, which utilizes the hydroponic symbiotic microbial system described in any one of the above, and comprises the following steps:

[0011] Place the hydroponic symbiotic microbial system on a rock wool block or a planting sponge;

[0012] Transplant a plant seedling with a root length of 1-3 cm onto the rock wool block or the planting sponge for cultivation.

[0013] Optionally, the hydroponic symbiotic microbial system is obtained through the following steps:

[0014] Collect root-soil mixture samples containing arbuscular mycorrhizal fungal communities, extract arbuscular mycorrhizal fungal community spores, and obtain an arbuscular mycorrhizal fungal community spore suspension; take samples containing Scopulariopsis, Aspergillus, Pseudomonas, and Gemmatimonas, add distilled water, stir, let stand, and filter to obtain a suspension containing Scopulariopsis, Aspergillus, Pseudomonas, and Gemmatimonas; mix the arbuscular mycorrhizal fungal community spore suspension with the suspension containing Scopulariopsis, Aspergillus, Pseudomonas, and Gemmatimonas to obtain a hydroponic symbiotic microbial system.

[0015] Optionally, wet-sieving decantation sucrose centrifugation, differential centrifugation, or ultrafiltration is used to extract arbuscular mycorrhizal fungal community spores.

[0016] Optionally, the pH of the arbuscular mycorrhizal fungal community spore suspension is 6 to 10.

[0017] Optionally, rock wool blocks or planting sponges are arranged in the hydroponic device.

[0018] Optionally, the hydroponic device includes a scientific research-level hydroponic device and a production-level hydroponic device. The scientific research-level hydroponic device includes centrifuge tubes and centrifuge tube racks wrapped with tin foil, and the production-level hydroponic device includes a hydroponic box and a planting basket.

[0019] Optionally, the cultivation conditions for the cultivation step are as follows: maintain 12 to 14 hours of light per day, the light intensity is 5000 lx, the temperature is 20 - 25 °C, and Hoagland nutrient solution lacking phosphorus is supplemented.

[0020] The beneficial effects of a hydroponic symbiotic microbial system and a hydroponic symbiotic method of the present invention are as follows:

[0021] Arbuscular mycorrhizal fungal communities can enhance the host's ability to cope with stresses such as salinity, heavy metals, and pests and diseases through various ways. Arbuscular mycorrhizal fungal communities improve the stress resistance of host plants in a hydroponic environment, which involves reducing damage, increasing photosynthetic intensity, promoting morphogenesis, and improving plant growth. It can be applied to the research on stress problems related to arbuscular mycorrhizal fungal communities and can also be applied to the stress resistance regulation of hydroponically produced fruits and vegetables, which is beneficial to reducing the use of chemical pesticides and achieving the development goal of sustainable agriculture.

[0022] Fungi of the genus Scopulariopsis can tolerate high concentrations of heavy metal antimony and show adsorption characteristics for different valence ions, demonstrating important ecological benefits in environmental purification and soil and water body restoration.

[0023] Fungi of the genus Aspergillus are widely distributed on grains, air, soil, and various organic substances, playing an important role in the decomposition and cycling of organic substances, contributing to maintaining the material balance of the ecosystem and soil fertility, and also playing a role in industrial applications, environmental purification, and scientific research.

[0024] Pseudomonas bacteria have low nutritional requirements and can grow in various environments such as soil, water, and air. They can degrade a variety of organic substances, and Pseudomonas strains also have the potential to remove heavy metals and organic pollutants, and can be used for the bioremediation of environmental pollution. In agriculture, Pseudomonas has the function of preventing diseases and promoting growth. Such strains can produce a variety of substances with antibacterial activity and have an inhibitory effect on a variety of plant pathogens. At the same time, Pseudomonas can also promote plant growth and improve the yield and quality of crops.

[0025] Gemmatimonas bacteria are widely distributed in various soils and play an important role in the stability and function of the ecosystem. Their strong nitrogen-fixing ability can improve soil fertility and crop yield. Their biological control function helps to protect plant health, reduce the use of pesticides, and lower agricultural production costs. At the same time, it also helps to maintain the balance of the ecological environment. Some strains can also decompose organic substances and convert them into nutrients such as inorganic salts required by plants.

[0026] The microbial systems of Sarocladium, Aspergillus, Pseudomonas, and Gemmatimonas enrich the microbial selection for hydroponic symbiosis. The microorganisms of Sarocladium, Aspergillus, Pseudomonas, and Gemmatimonas, as well as arbuscular mycorrhizal fungi, are common bacteria and fungi in the soil and all have the ability to decompose organic matter. However, their decomposition methods and products are different. The synergistic effect of the two can more effectively decompose organic matter and promote nutrient cycling. The synergistic secretion of antibacterial substances by various groups will also greatly enhance the disease resistance of plants and reduce the occurrence of diseases. There is also a synergistic effect between arbuscular mycorrhizal fungi and the four genera of Sarocladium, Aspergillus, Pseudomonas, and Gemmatimonas. The main manifestation is that arbuscular mycorrhizal fungi secrete organic substances to be used as the carbon and nitrogen sources for Pseudomonas and Gemmatimonas bacteria, promoting their growth and reproduction. Pseudomonas and Gemmatimonas bacteria provide nutrients for arbuscular mycorrhizal fungi by decomposing organic matter, thus forming a mutually beneficial symbiotic relationship. At the same time, arbuscular mycorrhizal fungi and other fungi, Sarocladium and Aspergillus, form a powerful network, which can improve the plant's ability to absorb nutrients. Further, Pseudomonas and Gemmatimonas bacteria decompose organic matter into small molecule substances through the action of extracellular enzymes, which can be directly absorbed and utilized by plants and other microorganisms. The arbuscular mycorrhizal fungal community, Sarocladium, and Aspergillus mainly decompose organic matter through the action of their hyphae and spores to form humus that can play a long-term role. The two work together to exert ecological benefits on different time scales. Based on this, the arbuscular mycorrhizal fungal community, Sarocladium, Aspergillus, Pseudomonas, and Gemmatimonas, by constructing a diverse microbial community, simulating the microbial structure in the natural environment, and giving full play to the synergistic effect of the microbial community, can interact with each other by jointly decomposing organic matter, promoting nutrient absorption, promoting biological control, and enhancing plant stress resistance, etc., to jointly maintain the stability of the ecosystem and promote plant growth. Specific implementation manners

[0027] Unless otherwise defined, all technical and scientific terms used in this invention have the same meanings as commonly understood by those skilled in the technical field to which this invention belongs. The terms used in the description of the invention in this invention are only for the purpose of describing specific embodiments, and are not intended to limit this invention.

[0028] As used herein, the term "comprising" and its variations are open-ended, that is, "including but not limited to"; the term "based on" means "at least partially based on"; the term "one embodiment" means "at least one embodiment"; the term "another embodiment" means "at least one additional embodiment"; the term "some embodiments" means "at least some embodiments"; the term "optionally" means "optional embodiments". The relevant definitions of other terms will be given in the following description.

[0029] In the related art, the synergistic action of microbial groups in the soil often has significant ecological benefits. They convert organic matter into nutrients that are easily absorbed by plants, and can improve environmental pH, etc., providing better conditions for plant growth. The synergistic action of microorganisms can also enhance the anti-disturbance ability of the cultivation environment, maintain ecological balance, and reduce the impact of abiotic and biotic factors on plant growth. The synergistic application of microorganisms can also produce substances with the characteristics of biological pesticides, effectively combat pathogens and pests, and promote the development of clean agriculture.

[0030] In view of the problems existing in the above-mentioned related art, this embodiment provides a hydroponic symbiotic microbial system and a hydroponic symbiotic method.

[0031] A hydroponic symbiotic microbial system provided by an embodiment of the present invention includes: an arbuscular mycorrhizal fungal community, Sarocladium, Aspergillus, Pseudomonas, and Gemmatimonas.

[0032] In this embodiment, the arbuscular mycorrhizal fungal community can enhance the host's ability to cope with stresses such as salinity, heavy metals, and pests and diseases through various ways. The arbuscular mycorrhizal fungal community improves the stress resistance of host plants in a hydroponic environment, which involves reducing damage, increasing photosynthetic intensity, promoting morphogenesis, and improving plant growth. It can be applied to the research on stress problems related to arbuscular mycorrhizal fungal communities, and can also be applied to the stress resistance regulation of hydroponically produced fruits and vegetables, which is beneficial to reducing the use of chemical pesticides and achieving the development goal of sustainable agriculture.

[0033] Fungi of the genus Sarocladium can tolerate high concentrations of heavy metal antimony and show adsorption characteristics for different valence ions, showing important ecological benefits in environmental purification and soil and water body remediation.

[0034] Aspergillus fungi are widely distributed on grains, in the air, in the soil, and on various organic substances. They play an important role in the decomposition and cycling of organic matter, contributing to maintaining the material balance of the ecosystem and soil fertility. They also play a role in industrial applications, environmental purification, and scientific research, etc.

[0035] Pseudomonas bacteria have low nutritional requirements and can grow in various environments such as soil, water, and air. They can degrade a variety of organic substances, and Pseudomonas strains also have the potential to remove heavy metals and organic pollutants and can be used for the bioremediation of environmental pollution. In agriculture, Pseudomonas has the effect of preventing diseases and promoting growth. Such strains can produce a variety of substances with antibacterial activity and have an inhibitory effect on a variety of plant pathogens. At the same time, Pseudomonas can also promote plant growth and improve the yield and quality of crops.

[0036] Gemmatimonas bacteria are widely distributed in various soils and play an important role in the stability and function of the ecosystem. Their strong nitrogen-fixing ability can improve soil fertility and crop yields. Their biocontrol effect helps to protect plant health, reduce the use of pesticides, lower agricultural production costs, and also helps to maintain the balance of the ecological environment. Some strains can also decompose organic substances and convert them into nutrients such as inorganic salts required by plants.

[0037] Microbial systems of Sarocladium, Aspergillus, Pseudomonas, and Gemmatimonas enrich the microbial selection for hydroponic symbiosis. Microorganisms of Sarocladium, Aspergillus, Pseudomonas, and Gemmatimonas, as well as arbuscular mycorrhizal fungi, are common bacteria and fungi in the soil and all have the ability to decompose organic matter. However, their decomposition methods and products are different. The synergistic effect of the two can decompose organic matter more effectively, promote nutrient cycling, and the synergistic secretion of antibacterial substances of various groups will also greatly enhance the disease resistance of plants and reduce the occurrence of diseases. There is also a synergistic effect between arbuscular mycorrhizal fungi and the four genera of Sarocladium, Aspergillus, Pseudomonas, and Gemmatimonas. The main manifestation is that arbuscular mycorrhizal fungi secrete organic substances as the carbon and nitrogen sources for Pseudomonas and Gemmatimonas, promoting their growth and reproduction. Pseudomonas and Gemmatimonas provide nutrients for arbuscular mycorrhizal fungi by decomposing organic matter, thus forming a mutually beneficial symbiotic relationship. At the same time, arbuscular mycorrhizal fungi and other fungi of Sarocladium and Aspergillus form a powerful network, which can improve the nutrient absorption ability of plants. Further, Pseudomonas and Gemmatimonas decompose organic matter into small-molecule substances through the action of extracellular enzymes, which can be directly absorbed and utilized by plants and other microorganisms. The arbuscular mycorrhizal fungal community, Sarocladium, and Aspergillus mainly decompose organic matter through the action of their hyphae and spores to form humus that can play a long-term role. The two work together to exert ecological benefits on different time scales. Based on this, the arbuscular mycorrhizal fungal community, Sarocladium, Aspergillus, Pseudomonas, and Gemmatimonas, by constructing a diverse microbial community, simulating the microbial structure in the natural environment, and giving full play to the synergistic effect of the microbial community, can interact with each other by jointly decomposing organic matter, promoting nutrient absorption, promoting biological control, and enhancing plant stress resistance, etc., to jointly maintain the stability of the ecosystem and promote the growth of plants.

[0038] Optionally, the arbuscular mycorrhizal fungal community includes one or more of Glomus, Acaulospora, Rhizophagus, Septoglomus, Claroideoglomus, Diversispora, Ambispora, Paraglomus, Scutellospora, Pacispora, Funneliformis, Claroideoglomus etunicatum, and Gigaspora.

[0039] In this optional embodiment, according to the soil physical and chemical properties of the growth points of Leymus chinensis in the Songnen Plain, a root-soil mixture sample of the growth points of Leymus chinensis containing the arbuscular mycorrhizal fungal community in this area is collected. The combined configuration of the arbuscular mycorrhizal fungal community can improve the stress resistance of host plants in the hydroponic environment, which involves reducing damage, increasing photosynthetic intensity, promoting morphogenesis, and improving plant growth. It can be applied to the research on stress problems related to the arbuscular mycorrhizal fungal community and can also be applied to the stress resistance regulation of fruits and vegetables produced by hydroponics, which is beneficial to reducing the use of chemical pesticides and achieving the development goal of sustainable agriculture.

[0040] Optionally, Glomus includes one or more of Glomus reticulatum, Glomus bullatum, Glomus foveatum, Glomus microaggregatum, Glomus melanosporum, Glomus fasciculatum, Glomus tortuosum, Glomus macrocarpum, Glomus halonatum, Glomus multicaule, Glomus hyderabadensis, Glomus macrocarpum, Glomus minimum, and Glomus longisporum; Acaulospora includes one or more of Acaulospora colombiana, Acaulospora verrucosa, Acaulospora koskei, Acaulospora lacunosa, Acaulospora scrobiculata, Acaulospora denticulata, Acaulospora gerdmannii, Acaulospora morrowiae, Acaulospora bireticulata, and Acaulospora foveata; Entrophospora includes one or more of Entrophospora infrequens and Entrophospora clara; Septoglomus includes one or more of Septoglomus arenarium and Septoglomus constrictum; Claroideoglomus includes one or more of Claroideoglomus etunicatum, Claroideoglomus claroideum, and Claroideoglomus lamellosum; Diversispora includes Diversispora epigaea; Ambispora includes one or more of Ambispora leptoticha, Ambispora reticulata, Ambispora jenkinsii, and Ambispora dura; Paraglomus includes Paraglomus occultum; Scutellospora includes one or more of Scutellospora reticulata and Scutellospora nigra; Pacispora includes one or more of Pacispora chusqueae and Pacispora boliviana; Funneliformis includes one or more of Funneliformis geosporum and Funneliformis coronatum; Rhizophagus includes Rhizophagus intraradices; Glomerospora includes Glomerospora fallax; The arbuscular mycorrhizal fungal community includes at least three Glomeromycota.

[0041] A hydroponic symbiotic method provided by an embodiment of the present invention uses the hydroponic symbiotic microbial system described in any one of the above, and includes the following steps:

[0042] S1: Place the hydroponic symbiotic microbial system on a rock wool block or a planting sponge;

[0043] S2: Transplant plant seedlings with roots 1 - 3 cm long onto the rock wool block or the planting sponge for cultivation.

[0044] In this embodiment, the rock wool block or the planting sponge is used to fix the plant seedlings and maintain an appropriate supply of water and nutrients. Correctly controlling the relationship between the liquid level of the nutrient solution and the bottom of the rock wool block or the planting sponge directly affects the oxygen supply to the roots, water absorption, and plant health. The correct liquid level position is crucial for the development of plant roots. If the liquid level is too high, it may cause hypoxia in the roots of the plant seedlings, while if it is too low, it may cause the roots of the plant seedlings to be too dry to effectively absorb water and nutrients. Therefore, ensure that the culture liquid level is at the bottom of the rock wool block or the planting sponge, and keep the bottom of the rock wool block or the planting sponge in slight contact with the liquid level (i.e., capillary wetting) but not completely immersed.

[0045] Specifically, plant seeds are preferably induced to germinate until the root length reaches 1.5 cm.

[0046] Specifically, the rock wool blocks and the planting sponges are wetted in advance, and then a suspension of the hydroponic symbiotic microbial system is injected onto the rock wool blocks or the planting sponges with a pipette.

[0047] Specifically, the rock wool, made of basalt fibers, has the characteristics of strong water retention and good air permeability, ensuring that plants can obtain sufficient water during growth and helping the roots to breathe and maintain healthy growth.

[0048] The planting sponge can effectively fix the plants, prevent the vegetables from lodging, provide support for the base of the vegetable seedlings, ensure the stability of the plants during growth, and at the same time it has good water absorption and water retention, can maintain a moist environment for a long time, and provide the water required for plant growth. Its pore structure ensures the respiration of plant roots, provides sufficient growth space for the roots of the seedlings, and is conducive to the healthy growth of the seedlings.

[0049] Optionally, the hydroponic symbiotic microbial system is obtained through the following steps:

[0050] Collect a root soil mixture sample containing an arbuscular mycorrhizal fungal community, extract the arbuscular mycorrhizal fungal community spores to obtain an arbuscular mycorrhizal fungal community spore suspension; take a sample containing Scopulariopsis, Aspergillus, Pseudomonas, and Gemmatimonas, add distilled water, stir and then let it stand, and filter to obtain a suspension containing Scopulariopsis, Aspergillus, Pseudomonas, and Gemmatimonas; mix the arbuscular mycorrhizal fungal community spore suspension with the suspension containing Scopulariopsis, Aspergillus, Pseudomonas, and Gemmatimonas to obtain the hydroponic symbiotic microbial system.

[0051] Specifically, weigh 20 g of the root soil mixture sample of the arbuscular mycorrhizal fungal community to obtain 2 ml of spore suspension. Weigh 10 - 100 g of the soil sample containing Scopulariopsis, Aspergillus, Pseudomonas, and Gemmatimonas, add distilled water, stir and let it stand for 20 min, and then filter through a 38 - micron filter membrane to obtain 1 mL of the suspension containing Scopulariopsis, Aspergillus, Pseudomonas, and Gemmatimonas.

[0052] Optionally, the arbuscular mycorrhizal fungal community spores are extracted by the wet - sieving sucrose centrifugation method, differential centrifugation method, or ultrafiltration method.

[0053] The purpose of wet sieving: preliminarily separate particles of different sizes in the sample.

[0054] Pass the suspension containing the target particles (such as the root soil suspension of the arbuscular mycorrhizal fungal community) through a series of sieves with different pore sizes (such as 100μm, 400μm) for step - by - step filtration.

[0055] Rinse the sieve with distilled water or deionized water to remove impurities in the soil while retaining the arbuscular mycorrhizal fungal spores.

[0056] Purpose of decantation: After wet sieving, remove light impurities in the supernatant and retain the arbuscular mycorrhizal fungal spores at the bottom.

[0057] Add sucrose to the target portion after wet sieve decantation and let it stand (or centrifuge it at low speed for a short time) to concentrate the spores in a specific position.

[0058] The principle of sucrose density gradient centrifugation: use the density difference formed by the sucrose concentration gradient to separate particles of different densities or sizes.

[0059] Gradient preparation: Add 55% (m / v) sucrose solution (sterile and pH stable) into a centrifuge tube.

[0060] Sample loading: Mix the decanted sample in sucrose solution.

[0061] Sample standing: Let the mixed turbid solution stand for 100 minutes.

[0062] Ultracentrifugation: Parameters are adjusted according to the target particle size.

[0063] Spore recovery: After centrifugation, a separation zone is formed and the target components are collected by puncture or siphon method.

[0064] The advantages are high resolution, the ability to separate particles with small density differences (such as organelle subtypes, virus particles), and the ability to separate complex mixtures; sucrose is an osmotic active substance that maintains the integrity of organelle morphology. However, it is time-consuming and requires high equipment.

[0065] The advantages of differential centrifugation are simple operation and low cost, but the resolution is low.

[0066] The advantages of ultrafiltration are that it is fast and suitable for large volume samples; however, it is easy to clog the membrane pores.

[0067] Optionally, the pH of the arbuscular mycorrhizal fungal community spore suspension is between 6 and 10.

[0068] Specifically, for the root soil mixture samples collected in the wild, it is preferred that the spore density is greater than 20 spores per gram of soil, the pH of the sample is between 6 and 10, and the number of spore species is more than 10. The pH value in this range helps to maintain the activity and growth of arbuscular mycorrhizal fungi. Arbuscular mycorrhizal fungi can better form a symbiotic relationship with plant roots within this pH range, thereby improving the plant's nutrient absorption capacity and stress resistance. By adjusting the pH value of the spore suspension, the effect of the hydroponic symbiotic system can be optimized, promoting the healthy growth of plants. Specifically, the spore suspension of the arbuscular mycorrhizal fungal community with a pH value between 6 and 10 can be achieved in the following ways: using buffer solutions, such as phosphate buffer solution, citrate buffer solution, etc., to adjust the pH value of the spore suspension. Adding acids or alkalis, as needed, the pH value of the suspension can be adjusted by adding appropriate amounts of acids (such as hydrochloric acid) or alkalis (such as sodium hydroxide). Using pH regulators, commercially available pH regulators can be used to quickly and accurately adjust the pH value of the suspension. Thus, the spore suspension of the arbuscular mycorrhizal fungal community with a pH value of 6 to 10 can significantly improve the stability and effectiveness of the hydroponic symbiotic system. Compared with the prior art, the present application solves the problem of low activity of arbuscular mycorrhizal fungi in traditional hydroponic devices by optimizing the pH value of the spore suspension, thereby improving the growth efficiency and stress resistance of plants

[0069] Optionally, rock wool blocks or planting sponges are arranged inside the hydroponic device.

[0070] Specifically, the hydroponic device refers to the structure for placing rock wool blocks or planting sponges, and does not include rock wool blocks or planting sponges and the microbial system for hydroponic symbiosis.

[0071] Optionally, the hydroponic device includes a scientific research-level hydroponic device and a production-level hydroponic device. The scientific research-level hydroponic device includes centrifuge tubes and centrifuge tube racks wrapped with tinfoil, and the production-level hydroponic device includes hydroponic boxes and planting baskets.

[0072] In this optional embodiment, the scientific research-level hydroponic device and the production-level hydroponic device are set up to meet different application requirements. The scientific research-level hydroponic device is mainly used for experimental research. The centrifuge tubes and the centrifuge tube racks wrapped with tin foil can be used for small-scale experiments, providing precise experimental conditions, reducing external interference, and helping researchers conduct detailed observations and data collection. The hydroponic boxes and the planting baskets in the production-level hydroponic device are more suitable for large-scale production. The hydroponic boxes and the planting baskets can provide a stable growth environment, facilitating management and operation. The centrifuge tubes in the scientific research-level hydroponic device can be used to culture and observe the growth of microorganisms and plant roots. The centrifuge tube racks wrapped with tin foil can prevent light interference and ensure the accuracy of experimental results. The hydroponic boxes and the planting baskets in the production-level hydroponic device are reasonably designed and can provide good ventilation and drainage conditions, promoting the healthy growth of plants. Thus, by setting up the scientific research-level hydroponic device and the production-level hydroponic device, the requirements for hydroponic conditions in different application scenarios are solved, further improving the applicability and flexibility of hydroponic technology. This embodiment is not only applicable to experimental research but also can meet the needs of large-scale production, having high practical value

[0073] Specifically, the centrifuge tube rack should be adapted to the centrifuge tube and is used to fix and support the corresponding centrifuge tube.

[0074] The tin foil strictly wraps the periphery and voids of the centrifuge tube rack, which is used to reflect light, create a dark environment for root growth, prevent the growth of algae, and ensure the accuracy of experimental results.

[0075] Specifically, for the scientific research-level hydroponic device, the construction steps are as follows:

[0076] After making planting holes in the pre-wetted rock wool blocks with tweezers, place the selected germinated seeds into the holes, and inject the liquid inoculum of the hydroponic symbiotic microbial system obtained into the rock wool blocks from the planting holes, ensuring that no liquid drips. Then place them into a 50 ml centrifuge tube filled with culture solution and insert the centrifuge tube rack.

[0077] Specifically, for the production-level hydroponic device, the construction steps are as follows:

[0078] Fix the selected germinated seeds in the planting sponge, then place the sponge into the planting basket, and inject the liquid inoculum of the hydroponic symbiotic microbial system obtained into the sponge, ensuring that no liquid drips. Then move this part to the hydroponic box and keep the liquid level lower than the lower part of the planting basket in the early stage.

[0079] Optionally, the culture conditions for the steps of cultivation are: maintaining 12 to 14 hours of light per day, a light intensity of 5000 lx, a temperature of 20 - 25 °C, and supplementing the Hoagland nutrient solution lacking phosphorus.

[0080] In this optional embodiment, the cultivation conditions of plant seedlings are optimized by controlling the light duration, light intensity, temperature, and the composition of the nutrient solution to promote the growth and development of plants. Specifically, a 12- to 14-hour light exposure per day can ensure sufficient photosynthesis in plants; a light intensity of 5000 lx can provide appropriate light energy; maintaining the temperature at 20-25 °C is conducive to the normal growth of plants; supplementing the phosphorus-deficient Hoagland nutrient solution can ensure that plants obtain the necessary nutrients and accelerate the symbiosis between arbuscular mycorrhizal fungi and plants, thereby promoting the healthy growth of roots and plants. The cultivation conditions in this technical solution can be achieved in the following ways: First, a timing switch and a light intensity regulator can be used to control the light duration and intensity; second, a thermostatic device can be used to maintain the temperature between 20-25 °C; finally, by regularly adding the prepared phosphorus-deficient Hoagland nutrient solution, a stable nutrient supply for plants is ensured. This embodiment improves the growth efficiency and health of plant seedlings in a hydroponic environment by precisely controlling the cultivation conditions, overcoming the problem of inaccurate control of environmental conditions in traditional hydroponic technologies. Thus, this embodiment realizes the precise regulation of plant growth factors, significantly enhancing the stability and reliability of the hydroponic system.

[0081] Specifically, the plant cultivation conditions are 14 hours of light exposure per day, a light intensity of 5000 lx, a temperature of 25 °C, and the phosphorus-deficient Hoagland nutrient solution is regularly irrigated once a week.

[0082] The present invention will be further described below with reference to specific embodiments.

[0083] In the embodiment of the present application, river sand is used as a control cultivation substrate. The pH value of river sand is usually neutral or nearly neutral, with simple chemical components, relatively stable properties and easy to control, and can accurately evaluate the experimental results.

[0084] The Leymus chinensis in the embodiment of the present application is a high-quality forage grass with high nutritional value and good palatability, suitable for the consumption of livestock such as cattle and sheep. It also has extremely strong environmental adaptability and can grow in various harsh environments such as saline-alkali, drought, and severe cold. At the same time, Leymus chinensis has broad soil requirements, is tolerant of barrenness and wind and sand, and can grow in plains, slopes, and sandy loam soils. It is the dominant forage grass in the Songnen Plain.

[0085] The saline-alkali stress solution in the embodiment of the present application is prepared according to the physical and chemical properties of the soil at the growth point of Leymus chinensis in the Songnen Plain. The main salt components in the saline-alkali soil in this area are carbonate and bicarbonate. In the embodiment of the present application, a stress solution with a pH value of 9.29 is prepared using Na2CO3 and NaHCO3.

[0086] The nutrient solution in the embodiments of the present application is of a phosphorus-free type, heated and dissolved in distilled water, and used after autoclaving at 115°C for 20 minutes. As a compound fertilizer and soilless culture nutrient solution widely used in the agricultural field, it is composed of various inorganic salts and water-soluble trace elements. By precisely proportioning these components, it provides comprehensive and balanced nutritional support for plants.

[0087] Table 1 Composition table of the arbuscular mycorrhizal fungal community of the root-soil mixture used in the embodiments of the present application

[0088]

[0089]

[0090]

[0091] Example 1

[0092] The Leymus chinensis seeds were disinfected with 2% NaClO3 and then placed in an incubator to induce germination, maintaining a completely dark state and a temperature change treatment of 20°C for 12 hours to 30°C for 12 hours per day until the root length reached 1.5 cm.

[0093] A root-soil mixture sample containing the arbuscular mycorrhizal fungal community at the growth point of Leymus chinensis in the Songnen Plain was collected. The arbuscular mycorrhizal (AM) fungal community spores were extracted from 20 g of the mixture sample by wet sieving decantation-sucrose centrifugation to obtain a liquid AM fungal community spore suspension, i.e., 2 - 3 ml of the liquid AM fungal community inoculant.

[0094] Set up a scientific research-level hydroponic device: Pre-wet the rock wool blocks and make planting holes with tweezers. After placing the selected germinated seeds into the planting holes, the above-mentioned AM fungal community inoculant was injected into the rock wool blocks from the planting holes using a 1000 μl pipette gun, ensuring no liquid dripping. Then it was placed into a 50 ml centrifuge tube filled with nutrient solution and inserted into a centrifuge tube rack. To prevent interference from factors such as light on the growth of plant roots, the tube rack was wrapped with a layer of tin foil.

[0095] Set up a soil culture system control group: A flower pot with a diameter of 12 cm and a height of 10.3 cm was used as the cultivation container, river sand was used as the cultivation substrate, and 20 g of the root-soil mixture containing the arbuscular mycorrhizal (AM) fungal community at the growth point of Leymus chinensis in the Songnen Plain was used as the inoculant. 6 germinated Leymus chinensis plants were transplanted into each pot.

[0096] Both the scientific research-level hydroponic device and the soil culture system control group were maintained with 14 hours of light per day, a light intensity of 5000 lx, a temperature of 25°C, and the phosphorus-deficient Hoagland nutrient solution was supplemented regularly.

[0097] After 70 days of growth in the hydroponic device and soil culture system, a saline-alkali stress with a pH of 9.29 was applied for 7 days. The saline-alkali stress solution was prepared according to the physical and chemical properties of the soil. The main salt components in the saline-alkali soil in this area are carbonate and bicarbonate. In this example, Na2CO3 and NaHCO3 were used to prepare a stress solution with a pH of 9.29. Then, the mycorrhizal infection, plant damage, and growth of the two systems were evaluated. The results are shown in Tables 2 and 3.

[0098] Table 2 Indexes of root infection and mycorrhizal function of Leymus chinensis in hydroponic and soil culture systems

[0099]

[0100] As can be seen from Table 2, after 77 days of cultivation and stress treatment of Leymus chinensis, the AM fungal communities in both the hydroponic system and the soil culture system successfully infected the host roots. And by using the method of this example, although its infection rate did not reach the level when planted in the soil culture system, its value could reach 72.58% in a short time. In addition, we calculated the mycorrhizal dependence of different groups based on biomass and found that in the hydroponic system, the mycorrhizal dependence of Leymus chinensis plants was stronger in the face of adversity stress, directly indicating that the AM fungal community participated in the regulation of the host's adversity response in this application, and its regulatory role in the hydroponic device was more significant.

[0101] Set up the hydroponic stress AM group: After 70 days of growth of the above scientific research-level hydroponic device, apply a saline-alkali stress with a pH of 9.29 for 7 days.

[0102] Set up the hydroponic stress group: Pre-wet the rock wool blocks, and use tweezers to make planting holes. After putting the selected germinated seeds into the planting holes, then place them in a 50 ml centrifuge tube filled with culture solution and insert it into the centrifuge tube rack. To prevent interference from factors such as light on the growth of plant roots, the tube rack is wrapped with a layer of tin foil. After 70 days of growth, apply a saline-alkali stress with a pH of 9.29 for 7 days.

[0103] Set up the hydroponic control group: Pre-wet the rock wool blocks, and use tweezers to make planting holes. After putting the selected germinated seeds into the planting holes, then place them in a 50 ml centrifuge tube filled with culture solution and insert it into the centrifuge tube rack. To prevent interference from factors such as light on the growth of plant roots, the tube rack is wrapped with a layer of tin foil. Grow for 77 days.

[0104] Set up the soil culture stress AM group: After 70 days of growth of the above soil culture system control group, apply a saline-alkali stress with a pH of 9.29 for 7 days.

[0105] Set up the soil culture stress group: Use flowerpots with a diameter of 12 cm and a height of 10.3 cm as cultivation containers, river sand as the cultivation substrate, and 20 g of a root-soil mixed sample of Leymus chinensis from the Songnen Plain, which has been sterilized by high-temperature and high-pressure treatment of the growth points, as an additive. Transplant 6 germinated Leymus chinensis plants into each pot. After growing for 70 days, apply saline-alkali stress with a pH of 9.29 for 7 days.

[0106] Set up the soil culture control group: Use flowerpots with a diameter of 12 cm and a height of 10.3 cm as cultivation containers, river sand as the cultivation substrate, and 20 g of a root-soil mixed sample of Leymus chinensis from the Songnen Plain, which has been sterilized by high-temperature and high-pressure treatment of the growth points, as an additive. Transplant 6 germinated Leymus chinensis plants into each pot. Grow for 77 days.

[0107] Table 3 Damage and growth index table of Leymus chinensis under different groups

[0108]

[0109]

[0110] The changes in the damage and growth indexes of Leymus chinensis in different treatment groups are shown in Table 3. It can be seen that whether in the hydroponic or soil culture system, after 7 days of saline-alkali stress, the content of superoxide anions, relative damage value, and malondialdehyde content in plants increase, while the fresh weight of plants, plant water content, and net photosynthetic rate decrease. However, in the treatment groups of hydroponic stress AM group and soil culture stress AM group inoculated with AM fungal communities, compared with the hydroponic stress group and soil culture stress group, the adversity damage is alleviated, and the values approach those of the hydroponic control group and soil culture control group, and even better than those of the hydroponic control group and soil culture control group. When there is no inoculation of AM fungi and no stress application, the net photosynthetic rate and water content of Leymus chinensis in the hydroponic control group are higher than those in the soil culture control group, which also proves that hydroponics is beneficial to shortening the plant growth cycle and nutrient accumulation.

[0111] Example 2

[0112] Different from Example 1, in Example 2, a production-level hydroponic device is used to replace the scientific research-level hydroponic device. Set up the production-level hydroponic device: Soak the planting sponge in advance, fix the selected germinated seeds in the planting sponge, then place the planting sponge in the planting basket, and use a 1000 μl pipette gun to inject the AM fungal community inoculum of Example 1 into the planting sponge to ensure that no liquid drips. Then move this part to the hydroponic box, and keep the liquid level lower than the lower part of the planting basket in the early stage.

[0113] Other steps of Example 2 are the same as those of Example 1.

[0114] After the hydroponic device and soil culture system grow for 70 days, apply saline-alkali stress with a pH of 9.29 for 7 days. Then evaluate the mycorrhizal infection, plant damage, and growth of the two systems. The results are shown in Table 4 and Table 5.

[0115] Table 4 Index table of root infection and mycorrhizal effect of Leymus chinensis under hydroponic and soil culture systems

[0116]

[0117] Table 4 shows the mycorrhizal infection and mycorrhizal effect of the roots of Leymus chinensis in the hydroponic device and soil culture system applicable to large-scale production. The AM fungal community successfully infected the roots of Leymus chinensis in the hydroponic device, with an infection rate reaching 60%, and the mycorrhizal dependence in this hydroponic device reached 2.15, which was 52.11% higher than that of the traditional soil cultivation method. This indicates that when facing stress, the AM fungi in the hydroponic device played a more significant role.

[0118] The establishment of the groups in Table 5 is the same as that in Example 1, and only the scientific research-level hydroponic device is replaced with a production-level hydroponic device for detection.

[0119] Table 5 Index table of damage and growth of Leymus chinensis under different groups

[0120]

[0121] As can be seen from Table 5, the net photosynthetic rate reflects the difference between the total amount of organic matter produced by photosynthesis per unit time and the amount of organic matter consumed by respiration in plants, which is crucial for understanding the growth and development process of plants. In this device, the net photosynthetic rate of the hydroponic control group was higher than that of the soil culture control group, but showed the opposite trend after being subjected to saline-alkali stress, and the photosynthetic intensity value of the hydroponic stress group increased after inoculating AM fungi, which was 56.36% higher than that of the soil culture stress AM group. In this device, superoxide anion, relative damage value, malondialdehyde content, plant fresh weight, water content, and plant greenness value showed a similar trend to that in Example 1. The above results indicate that the AM fungal community also played a role in this hydroponic system.

[0122] Through the research of Example 1 and Example 2, it was found that under hydroponic conditions, plants have higher photosynthetic efficiency and growth and development potential, and the AM fungal community can be applied to hydroponic research after obtaining liquid inoculum by wet sieving, decanting, and sucrose centrifugation. It can not only promote plant morphogenesis and improve metabolism, but also improve the stress response of plants when facing stress.

[0123] Example 3

[0124] The hydroponic stress AM group is from Example 1.

[0125] Hydroponic stress AM + other microbiomes. Set up a scientific research-level hydroponic device as in Example 1. Pre-wet the rock wool blocks, and use tweezers to make planting holes. After placing the selected germinated seeds into the planting holes, a suspension mixture of the AM fungal community inoculum in Example 1 and other microorganisms including the genera Sarocladium, Aspergillus, Pseudomonas, and Gemmatimonas is used to form a hydroponic symbiotic microbial system. The hydroponic symbiotic microbial system is injected into the rock wool blocks from the planting holes using a 1000 μl pipette gun, ensuring no liquid drips. Subsequently, place it into a 50 ml centrifuge tube filled with culture solution and insert it into a centrifuge tube rack. To prevent interference from factors such as light on the growth of plant roots, the tube rack is wrapped with a layer of tin foil.

[0126] Maintain 14 hours of light per day, a light intensity of 5000 lx, a temperature of 25 °C, and regularly supplement the phosphorus-deficient Hoagland nutrient solution.

[0127] After 70 days of growth in the hydroponic AM group and the hydroponic AM + other microbiome group, apply saline-alkali stress with a pH of 9.29 for 7 days. Then evaluate the mycorrhizal infection, plant damage, and growth of the two groups. The results are shown in Tables 6 and 7.

[0128] Table 6 Indexes of root infection and mycorrhizal function of Leymus chinensis under different groups

[0129]

[0130] Table 6 shows the infection status of AM fungi in the scientific research-level hydroponic system. Among them, the data of the hydroponic stress AM group come from Example 1. After adding other microbial groups, the infection rate of AM fungi increases, and the mycorrhizal dependency value of Leymus chinensis also rises, indicating that the microbial synergy of the hydroponic symbiotic microbial system further mobilizes the ecological benefits and biological regulatory effects of arbuscular mycorrhizal fungi.

[0131] Table 7 Damage and growth indexes of Leymus chinensis under different groups

[0132]

[0133] Table 7 shows the growth and physiology of Leymus chinensis in different inoculation treatment groups in the scientific research-level hydroponic system. The data of the hydroponic stress AM group come from Example 1. When adding the genera Sarocladium, Aspergillus, Pseudomonas, and Gemmatimonas and inoculating AM, the damage of Leymus chinensis under saline-alkali stress is lower compared to the group only inoculated with AM fungi, and the growth state of the plants is further improved, manifested as an increase in the fresh weight of the plants under saline-alkali stress and a 6.78% increase in the plant water content. At the same time, the microbial synergy of the hydroponic symbiotic microbial system improves the net photosynthetic rate of the plants.

[0134] Example 4

[0135] The hydroponic stress AM group is from Example 2.

[0136] For the hydroponic stress AM + other microbiome group, a production-level hydroponic device as in Example 2 was set up. The planting sponge was pre-soaked, and the selected germinated seeds were fixed in the planting sponge. Subsequently, the planting sponge was placed in the planting basket, and a suspension of the AM fungal community inoculum from Example 1 and other microorganisms including Gliocladium, Aspergillus, Pseudomonas, and Gemmatimonas was mixed to form a hydroponic symbiotic microbial system. The hydroponic symbiotic microbial system was injected into the planting sponge using a 1000 μl pipette gun to ensure no liquid dripping. Then this part was transferred to the hydroponic box, and the liquid level was kept lower than the lower part of the planting basket in the early stage.

[0137] Maintain 14 hours of light per day, a light intensity of 5000 lx, a temperature of 25 °C, and regularly supplement the phosphorus-deficient Hoagland nutrient solution.

[0138] After 70 days of growth in the hydroponic AM group and the hydroponic AM + other microbiome group, a saline-alkali stress with a pH of 9.29 was applied for 7 days. Then the mycorrhizal infection, plant damage, and growth of the two systems were evaluated. The results are shown in Tables 8 and 9.

[0139] Table 8 Indexes of root infection and mycorrhizal function of Leymus chinensis under different groups

[0140]

[0141] Table 8 shows the changes in mycorrhizal infection rate and mycorrhizal dependency in the production-level hydroponic device. The data of the hydroponic stress AM group were from Example 2. When multiple microbial groups were applied synergistically, the value of the infection rate of AM fungi further increased, and the mycorrhizal dependency reached 2.65, indicating that when other bacteria and fungi were involved, the host plant had a strong dependence on the infection of arbuscular mycorrhizal fungi to obtain the maximum growth or yield, and the synergistic effect of the hydroponic symbiotic microbial system enhanced the ecological benefits of AM fungi in this hydroponic device.

[0142] Table 9 Damage and growth indexes of Leymus chinensis under different groups

[0143]

[0144] Table 9 shows the numerical changes in adversity damage indicators, morphological growth indicators, photosynthetic indicators, etc. of Leymus chinensis grown in a hydroponic device for water production after 7 days of saline-alkali stress. The data of the hydroponic stress AM group come from Example 2. Compared with the treatment group inoculated only with AM fungi, when multiple types of microorganisms act synergistically, the content value of superoxide anion decreases by 25%, indicating that the antioxidant system can effectively scavenge free radicals in the plant, thereby protecting cells from oxidative damage. In addition, when multiple groups are added simultaneously, the fresh weight and water content of Leymus chinensis also increase significantly, with the values reaching 197.5 mg and 52% respectively, an increase of 7.34% and 67.74%. In addition, the combined action of the hydroponic symbiotic microbial system can also effectively increase the net photosynthetic rate of plants and reduce the yellowing degree of plant leaves under adversity. The above results show that adding bacteria and fungi of the genera Sarocladium, Aspergillus, Pseudomonas, and Gemmatimonas while inoculating AM fungi can not only enhance the effect of AM fungi but also improve the stress resistance and growth potential of plants.

[0145] In the examples of this application, Leymus chinensis was used as a model plant and the root-soil mixture of the Songnen Plain was used as the community inoculant for research, achieving the expected results. If the plant and inoculant sources in Examples 1, 2, 3, and 4 are changed, similar results to the four examples can also be obtained.

[0146] Although the present invention is disclosed as above, the protection scope of the present invention is not limited thereto. Those skilled in the art can make various changes and modifications without departing from the spirit and scope of the present invention, and these changes and modifications will all fall within the protection scope of the present invention.

Claims

1. A hydroponic symbiotic microbial system, characterized in that: include: Arbuscular mycorrhizal fungal communities, Scopulariopsis, Aspergillus, Pseudomonas, and Gemmatimonas genera.

2. The hydroponic symbiotic microbial system according to claim 1, characterized in that: The arbuscular mycorrhizal fungal community includes one or more of the genera Glomus, Asessile Mycocystis, Rhizosporium, Sept. Glomus, Near-clear Glomus, Diverse Sporangium, Dimorphomic Sporangium, Para-Glomus, Shield Giant Sporangium, Peaceful Sporangium, Pipeline Sporangium, Curved Sclerocystis and Giant Sporangium.

3. The hydroponic symbiotic microbial system according to claim 2, characterized in that: The genus Glomeromyces includes one or more of glomeromyces reticularis, glomeromyces with vesicles, glomeromyces pits, glomeromyces microplexus, glomeromyces blackspores, glomeromyces aggregates, glomeromyces curled, glomeromyces inflated, glomeromyces halo-globe, glomeromyces multipedes, glomeromyces hyderabadius, glomeromyces macrocarpon, glomeromyces minimus and glomeromyces longispore; the genus Asessiles includes one or more of Asessiles Columbiana, Asessiles Verrucosa, Asessiles Koch, Asessiles Shallow Pit, Asessiles Fine Concave, Asessiles Fine Denticulate, Asessiles Gedan, Asessiles Mao, Asessiles Double Net and Asessiles Pit; the genus Rhizosporangia includes one or more of Rhizosporangia Intraradica and Asessiles Bright; the genus Septum Glomeromyces includes one or more of Asessiles Sandy and Asessiles Contracting; the genus Near Bright The genus Spore Fungus includes one or more of the young set of Near-clear Spore Fungus, Near-clear Spore Fungus and Layered Near-clear Spore Fungus; the genus Diverse Spore Fungus includes the ivory white diverse spore fungus; the genus Dimorphic Spore Fungus includes one or more of thin-walled Dimorphic Spore Fungus, reticulated Dimorphic Spore Fungus, Janis Dimorphic Spore Fungus and Sclerostomycetes; the genus Paraspore Fungus includes Cryptospore Fungus; the genus Giant Spore Fungus includes one or more of Reticulated Giant Spore Fungus and Black Shield Giant Spore Fungus; the genus Peace Spore Fungus includes one or more of Square Bamboo Peace Spore Fungus and Bolivian Peace Spore Fungus; the genus Pipe Fungus includes one or more of Pipe Fungus and Pipe Fungus Paracrown; the Sclerostomycetes include Pipe Fungus Moses; the genus Giant Spore Fungus includes Mistake Giant Spore Fungus; the arbuscular mycorrhizal fungal community includes at least three types of Spore Fungus.

4. A hydroponic symbiotic method, characterized in that: The hydroponic symbiotic microbial system according to any one of claims 1 to 3 comprises the following steps: Placing the hydroponic symbiotic microbial system on a rock wool block or a colonization sponge; Plant seedlings with a root length of 1-3 cm are transplanted onto the rock wool block or the planting sponge for cultivation.

5. The hydroponic symbiotic method according to claim 4, characterized in that: The hydroponic symbiotic microbial system is obtained by the following steps: A root-soil mixture sample containing the arbuscular mycorrhizal fungal community is collected, spores of the arbuscular mycorrhizal fungal community are extracted, and a spore suspension of the arbuscular mycorrhizal fungal community is obtained; a sample containing the genus Scopulariopsis, the genus Aspergillus, the genus Pseudomonas, and the genus Gemmatimonas is added to distilled water, stirred, and then allowed to stand, and a suspension containing the genus Scopulariopsis, the genus Aspergillus, the genus Pseudomonas, and the genus Gemmatimonas is obtained after filtering; the spore suspension of the arbuscular mycorrhizal fungal community is mixed with a suspension containing the genus Scopulariopsis, the genus Aspergillus, the genus Pseudomonas, and the genus Gemmatimonas to obtain the hydroponic symbiotic microbial system.

6. The hydroponic symbiotic method according to claim 5, characterized in that: The spores of the arbuscular mycorrhizal fungal community are extracted by wet sieve decantation sucrose centrifugation, differential centrifugation or ultrafiltration.

7. The hydroponic symbiotic method according to claim 5, characterized in that: The pH of the spore suspension of the arbuscular mycorrhizal fungal community is 6 to 10.

8. The hydroponic symbiotic method according to claim 4, characterized in that: The rock wool block or the planting sponge is arranged in a hydroponic device.

9. The hydroponic symbiotic method according to claim 8, characterized in that: The hydroponic device includes a scientific research-level hydroponic device and a production-level hydroponic device, wherein the scientific research-level hydroponic device includes a centrifuge tube and a centrifuge tube rack wrapped with tin foil, and the production-level hydroponic device includes a hydroponic box and a planting basket.

10. The hydroponic symbiotic method according to claim 4, characterized in that: The culture conditions for the culture in the step are: maintaining light for 12 to 14 hours per day, a light intensity of 5000 lx, a temperature of 20-25° C., and supplementing with phosphorus-deficient Hoagland nutrient solution.

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