POCT blood cell analyzer and blood cell analysis method

Abstract

The invention discloses a POCT hematology analyzer and a hematology analysis method, a transshipment assembly of the hematology analyzer is arranged on a seat body, the transshipment assembly comprises a loading seat and a driving motor, the driving motor is used for driving the loading seat to move and receiving a kit, and a fluorescence detection card, an impedance detection assembly and a fluorescence detection assembly are arranged on the seat body. According to the POCT hematology analyzer, the loading seat is driven through the driving motor, so that the hematology analyzer can realize impedance detection of a kit and fluorescence detection of a fluorescence detection card through the driving motor, the hematology analyzer is compact in structure, the space occupied by the transshipment assembly is relatively small, the instrument cost of the hematology analyzer is reduced, and the detection efficiency is improved. And miniaturization application of the hematology analyzer is facilitated.

Description

Technical Field

[0001] This application relates to the technical field of medical devices, particularly to a POCT blood cell analyzer and a blood cell analysis method. Background Art

[0002] In the field of medical devices, a blood cell analyzer can cooperate with a reagent kit and a fluorescence detection card to perform point-of-care testing on a sample to be tested, so as to improve the detection speed and enable users to quickly obtain test results. Existing blood cell analyzers can be provided with an impedance detection component and a fluorescence detection component, so that users can test detection items on a sample to be tested through the blood cell analyzer.

[0003] However, when a blood cell analyzer adapts to different detection components, it usually needs to set a corresponding drive motor for each detection component to cooperate with sample testing, resulting in a relatively large volume and high instrument cost of the blood cell analyzer. Summary of the Invention

[0004] To solve the above problems existing in the prior art, this application provides a POCT blood cell analyzer and a blood cell analysis method.

[0005] To solve the technical problems existing in the prior art, this application provides a POCT blood cell analyzer, including a base body, a transfer assembly, an impedance detection component, and a fluorescence detection component. The transfer assembly is disposed on the base body. The transfer assembly includes a loading seat and a drive motor. The drive motor is used to drive the loading seat to move and receive a reagent kit and a fluorescence detection card. The impedance detection component and the fluorescence detection component are disposed on the base body. The drive motor is further used to move the reagent kit on the loading seat to the impedance detection component for impedance detection, and move the fluorescence detection card on the loading seat to the fluorescence detection component for fluorescence detection.

[0006] Optionally, the POCT blood cell analyzer further includes a sensor and a processor. The sensor is disposed on the base body and is used to detect whether the loading seat is at a first working position. The processor is respectively connected to the sensor and the drive motor. The processor is used to control the drive motor to move the loading seat at the first working position to a second working position, so that the impedance detection component performs impedance detection on the reagent kit. The processor is further used to control the drive motor to move the loading seat at the first working position to a third working position, so that the fluorescence detection component performs fluorescence detection on the fluorescence detection card.

[0007] Optionally, the second working position and the third working position are arranged side by side, or the second working position and the third working position are the same position.

[0008] Optionally, when the loading seat is located at the second working position, the impedance detection component further includes an electrode connecting member, and the electrode connecting member is electrically connected to the electrode on the reagent kit;

[0009] And / or, when the loading seat is located at the third working position, the fluorescence detection component further includes a light generator, and the detection light beam emitted by the light generator irradiates the detection area of the fluorescence detection card.

[0010] Optionally, the POCT blood cell analyzer further includes a liquid transfer component, the liquid transfer component is arranged on the seat body, the liquid transfer component includes a lifting motor and a liquid transfer member, the lifting motor is used to drive the liquid transfer member to move in the vertical direction, and the loading and unloading component is further used to move the reagent kit to below the liquid transfer member through the driving force of the driving motor, so that the liquid transfer member sequentially transfers the reagents and the samples to be tested in the reagent kit through the lifting motor.

[0011] Optionally, the POCT blood cell analyzer further includes a chamber door, the chamber door is arranged on the side wall of the seat body and is rotatably connected to the seat body, and the loading seat is used to push the chamber door under the drive of the driving motor, so that the chamber door rotates and the loading seat is partially or completely exposed outside the seat body.

[0012] Optionally, the loading seat includes a separately arranged first loading seat and a second loading seat, the first loading seat is used to receive the reagent kit, and the second loading seat is used to receive the fluorescence detection card.

[0013] Optionally, the second loading seat is further used to receive a biochemical detection card, a detection window is opened at the bottom of the second loading seat, the POCT blood cell analyzer further includes a biochemical detection component, the biochemical detection component is arranged at the bottom of the second loading seat and corresponds to the detection window, and is used to perform biochemical detection on the biochemical detection card in the second loading seat.

[0014] Optionally, the loading and unloading component includes a slide rail, an execution mechanism and a motion mechanism, the slide rail is arranged on the seat body, the motion mechanism is slidably connected to the slide rail, the loading seat is arranged on the motion mechanism, the execution mechanism includes a push rod and a flange member, the driving motor is connected to the push rod and is used to drive the push rod to rotate, the flange member is sleeved on the push rod and is connected to the motion mechanism, and the flange member is used to receive the rotational force of the push rod and drive the motion mechanism to move in the horizontal direction.

[0015] To solve the technical problems existing in the prior art, the present application provides a blood cell analysis method, which is applied to the above-mentioned POCT blood cell analyzer. The above-mentioned blood cell analysis method includes: moving the loading seat loaded with the reagent kit and the fluorescence detection card to the first working position; controlling the loading seat at the first working position to move a first number of steps to move the loading seat to the second working position and perform impedance detection on the reagent kit; controlling the loading seat at the second working position to return to the first working position, and controlling the loading seat at the first working position to move a second number of steps so that the loading seat is located at the third working position; performing fluorescence detection on the fluorescence detection card at the third working position.

[0016] The present application provides a POCT blood cell analyzer and a blood cell analysis method. The blood cell analyzer drives the loading seat through a driving motor, enabling the blood cell analyzer to perform impedance detection on the reagent kit and fluorescence detection on the fluorescence detection card through the driving motor. The structure of the blood cell analyzer is compact, the space occupied by the loading component is small, the instrument cost of the blood cell analyzer is reduced, and it is beneficial to the miniaturized application of the blood cell analyzer. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] In order to more clearly illustrate the technical solutions in the embodiments of the present application or the prior art, the following will briefly introduce the drawings required for use in the embodiments. Obviously, the drawings in the following description are only some embodiments of the present application. For those of ordinary skill in the art, without creative efforts, other drawings can also be obtained based on these drawings.

[0018] Figure 1 is a schematic structural diagram of an embodiment of the POCT blood cell analyzer provided by the present application;

[0019] Figure 2 is a schematic structural diagram of another embodiment of the POCT blood cell analyzer provided by the present application;

[0020] Figure 3 is Figure 2 a schematic structural diagram of the second loading seat in

[0021] Figure 4 is a schematic flowchart of an embodiment of the blood cell analysis method provided by the present application;

[0022] Figure 5 is a schematic structural diagram of an embodiment of the computer-readable storage medium provided by the present application. DETAILED DESCRIPTION OF THE EMBODIMENTS

[0023] The present application will be further described in detail below in conjunction with the accompanying drawings and embodiments. It should be specifically noted that the following embodiments are only used to illustrate the present application, but do not limit the scope of the present application. Similarly, the following embodiments are only partial embodiments of the present application rather than all embodiments. All other embodiments obtained by those of ordinary skill in the art without creative efforts fall within the scope of protection of the present application.

[0024] As used herein, the term "embodiment" means that a particular feature, structure, or characteristic described in connection with the embodiment can be included in at least one embodiment of the present application. The phrase appears in various places in the specification and does not necessarily refer to the same embodiment, nor is it an independent or alternative embodiment mutually exclusive with other embodiments. Those skilled in the art will explicitly and implicitly understand that the embodiments described herein can be combined with other embodiments.

[0025] In the description of the present application, it should be noted that unless otherwise clearly specified and limited, the terms "installed", "set", "connected", and "coupled" should be understood in a broad sense. For example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be directly connected or indirectly connected through an intermediate medium. For those of ordinary skill in the art, if the embodiments of the present application involve directional indications (such as up, down, left, right, front, back...), then the directional indications are only used to explain the relative positional relationship and movement conditions between components in a specific posture (as shown in the accompanying drawings). If the specific posture changes, the directional indications will also change accordingly.

[0026] Please refer to Figure 1 and Figure 2 , Figure 1 which are schematic structural diagrams of an embodiment of the POCT hematology analyzer provided by the present application, Figure 2 and Figure 1 and Figure 2 which are schematic structural diagrams of another embodiment of the POCT hematology analyzer provided by the present application. As shown in

[0027] The transfer assembly 20 is disposed on the base 10. The transfer assembly 20 includes a loading base 21 and a driving motor 22. The driving motor 22 is used to drive the loading base 21 to move and receive the reagent kit 211 and the fluorescence detection card 221. The impedance detection assembly 40 and the fluorescence detection assembly 51 are disposed on the base 10. The driving motor 22 is further used to move the reagent kit 211 on the loading base 21 to the impedance detection assembly 40 for impedance detection and move the fluorescence detection card 221 on the loading base 21 to the fluorescence detection assembly 51 for fluorescence detection.

[0028] Specifically, the transfer assembly 20 can drive the loading seat 21 through a driving motor, or can drive the loading seat 21 through multiple driving motors 22, which is not specifically limited herein. For example, the transfer assembly 20 can drive the loading seat 21 through a driving motor 22 to save driving space; or the transfer assembly 20 can also drive the loading seat 21 through multiple driving motors 22 to improve driving efficiency. When the kit 211 includes a plurality of cell pools arranged linearly, the cell pools include a detection cell and a non-detection cell. The non-detection cell is used to provide reagents, samples, etc. required for detection, and the detection cell is used to provide a detection site for blood cell analysis, so that the kit 211 can be used for cell counting tests. The fluorescence detection card 221 is a detection article for rapid testing using the fluorescence immunochromatography method. The fluorescence detection card 221 corresponds to the fluorescence detection assembly 51, and the fluorescence detection assembly 51 is used to read the fluorescence intensity of the detection area on the fluorescence detection card 221 and characterize the blood cell characteristics of the sample to be tested based on the fluorescence intensity.

[0029] The impedance detection assembly 40 can include an electrode connector and a CBC analog circuit board card. The CBC analog circuit board card is electrically connected to the electrode connector through a wire. The driving motor 22 is used to drive the loading seat 21 to move to the first detection position, so that the electrodes on the kit 211 are connected in alignment with the electrode connector, and the electrode connector is used to divert an external current signal or voltage signal to the kit 211. The voltage signal or current signal diverted by the electrode connector contacts the sample to be tested in the detection cell through the electrodes of the kit 211, so that the CBC analog circuit board card can characterize the blood cell characteristics by collecting the electrical pulse signals of the sample to be tested.

[0030] Exemplarily, the driving motor 22 is used to drive the loading seat 21 so that the kit 211 is electrically connected to the electrode connector, and the impedance detection assembly 40 performs impedance detection on the sample to be tested in the kit 211. After the impedance detection is completed, the driving motor 22 drives the loading seat 21 to the fluorescence detection assembly 51, and the fluorescence detection assembly 51 is used to perform optical detection on the detection area of the fluorescence detection card 221 to complete the fluorescence detection. Or, the driving motor 22 is used to drive the loading seat 21 to a preset position, at which both the fluorescence detection assembly 51 and the impedance detection assembly 40 perform tests. Or, during the process of the driving motor 22 driving the loading seat 21 from the current position to the impedance detection assembly 40, the fluorescence detection assembly 51 performs optical detection on the fluorescence detection card 221. It can be understood that the user can adjust the order of fluorescence detection and impedance detection by adjusting the installation positions of the fluorescence detection assembly 51 and the impedance detection assembly 40, the driving logic of the driving motor 22, etc., which is not specifically limited herein.

[0031] In an embodiment of the present application, the hematology analyzer drives the loading seat 21 through the drive motor 22, so that the hematology analyzer can perform impedance detection on the reagent kit 211 and fluorescence detection on the fluorescence detection card 221 through the drive motor 22. The structure of the hematology analyzer is compact, and the space occupied by the loading assembly 20 is small, reducing the instrument cost of the hematology analyzer and facilitating the miniaturized application of the hematology analyzer.

[0032] In one embodiment, the POCT hematology analyzer further includes a sensor 120 and a processor. The sensor 120 is disposed on the seat body 10 and is used to detect whether the loading seat 21 is in the first working position. The processor is respectively connected to the sensor 120 and the drive motor 22. The processor is used to control the drive motor 22 to move the loading seat 21 located in the first working position to the second working position, so that the impedance detection component 40 performs impedance detection on the reagent kit 211. The processor is also used to control the drive motor 22 to move the loading seat 21 located in the first working position to the third working position, so that the fluorescence detection component 51 performs fluorescence detection on the fluorescence detection card 221.

[0033] Specifically, the processor is respectively connected to the sensor 120, the drive motor 22, the impedance detection component 40, and the fluorescence detection component 51. The sensor 120 is used to detect whether the loading seat 21 is in the first working position. The sensor 120 can be, but is not limited to, an optocoupler sensor 120, a position detection sensor 120, etc. The first working position can be understood as the initial position for the drive motor 22 to perform drive control. Each time the drive motor 22 moves to other working positions for different operations, it needs to start moving from the first working position to ensure that the drive motor 22 can accurately move the loading seat 21 to the preset position, improving the position accuracy of the reagent kit 211 and the fluorescence detection card 221 on the loading seat 21. The processor is used to judge whether the loading seat 21 is in the first working position through the signal feedback of the sensor 120 and control the driving process of the drive motor 22.

[0034] In an alternative embodiment, when a fault such as a step loss occurs during the process of the controller controlling the movement of the drive motor 22, resulting in the controller being unable to obtain the current position of the loading seat 21, the controller can control the drive motor 22 to randomly move the loading seat 21 in a preset direction, so that the loading seat 21 triggers the detection of the sensor 120, realizes the positioning of the loading seat 21, and continues to perform drive control on the loading seat 21 from the first working position, further improving the position accuracy of the loading seat 21.

[0035] Among them, the processor in this embodiment is further configured to perform signal processing and conversion on the electrical pulse signal of the impedance detection component 40 and the fluorescence intensity of the fluorescence detection component 51, so as to obtain the detection result of the sample flow to be detected. The processor can be referred to as a CPU (Central Processing Unit); the processor can also be an electronic chip with signal processing capabilities; the processor can also be a general-purpose processor, a digital signal processor (DSP), an application-specific integrated circuit (ASIC), a field-programmable gate array (FPGA) or other programmable logic devices, discrete gate or transistor logic devices, discrete hardware components. General-purpose processors include but are not limited to microprocessors or conventional processors, etc.

[0036] Optionally, the second working position and the third working position are arranged side by side, or the second working position and the third working position are the same position.

[0037] Specifically, in a possible implementation manner, the second working position and the third working position are arranged side by side and do not overlap, so that the fluorescence detection component 51 and the impedance detection component 40 do not need to be compactly arranged in the same area, which is convenient for spatial layout; and, since the electrical pulse signal is relatively weak, the second working position and the third working position being arranged side by side can enable the fluorescence detection component 51 and the impedance detection component 40 not to perform simultaneously, reducing the interference degree of the electromagnetic signal on the impedance detection component 40 when the fluorescence detection component 51 performs fluorescence detection, and improving the accuracy of impedance detection.

[0038] Exemplarily, the second working position and the third working position being arranged side by side can be on the same side, or can be arranged on both sides. When the second working position and the third working position are arranged on both sides, the fluorescence detection component 51 and the impedance detection component 40 can be arranged separately on both sides, which is convenient for the user to reasonably arrange the space on the seat body to reduce the mutual interference between the fluorescence detection component 51 and the impedance detection component 40; and, separating the fluorescence detection component 51 and the impedance detection component 40 on both sides of the seat body can make the weight of the POCT blood cell analyzer evenly distributed, avoiding wear and affecting the docking accuracy due to excessive weight on one side after long-term operation.

[0039] In other embodiments, the second working position and the third working position can also be the same position, so that the drive motor 22 can perform impedance detection and fluorescence detection without moving the position, reducing the moving time and improving the detection efficiency.

[0040] Optionally, the loading seat 21 is located at the second working position. The impedance detection component 40 further includes an electrode connecting member, which is electrically connected to the electrodes on the reagent kit 211. Among them, the electrodes on the reagent kit 211 may include a third electrode and a fourth electrode. One of the third electrode and the fourth electrode is a negative electrode, and the other is a positive electrode. The detection pool of the reagent kit 211 is located between the third electrode and the fourth electrode. The voltage signal or current signal diverted by the electrode connecting member contacts the sample to be tested in the detection pool through the third electrode and the fourth electrode, so that the CBC analog circuit board can characterize the blood cell characteristics of the sample to be tested by collecting the electrical pulse signal between the third electrode and the fourth electrode. The driving motor 22 moves the loading seat 21 to the second working position and drives the electrodes of the reagent kit 211 to be connected to the electrode connecting member, realizing electrode alignment and assisting the impedance detection component 40 to detect the reagent kit 211 at the same time. It can reduce the setting of other alignment and matching structures, has a simple structure, and improves the utilization rate of the driving motor 22.

[0041] Optionally, the loading seat 21 is located at the third working position. The fluorescence detection component 51 further includes a light generator, and the detection light beam emitted by the light generator irradiates the detection area of the fluorescence detection card 221. Among them, the fluorescence detection component 51 may further include a light receiver. The light generator is used to emit a detection light beam so that the detection light beam irradiates the detection area of the fluorescence detection card 221. The sample to be tested emits fluorescence under the action of the detection light beam. The light receiver is used to receive the fluorescence of the sample to be tested and characterize the blood cell characteristics of the sample to be tested according to the fluorescence intensity. The driving motor 22 moves the loading seat 21 to the third working position and makes the detection light beam of the light emitter irradiate the fluorescence detection card 221, so as to assist the fluorescence detection process of the fluorescence detection component 51 through the movement of the driving motor 22. The detection process is simple and easy to implement, and further improves the utilization rate of the driving motor 22.

[0042] In one embodiment, the POCT blood cell analyzer further includes a liquid transfer component 30. The liquid transfer component 30 is arranged on the seat body 10. The liquid transfer component 30 includes a lifting motor 31 and a liquid transfer member 32. The lifting motor 31 is used to drive the liquid transfer member 32 to move in the vertical direction. The loading component 20 is further used to move the reagent kit 211 to the lower part of the liquid transfer member 32 through the driving force of the driving motor 22, so that the liquid transfer member 32 sequentially transfers the reagents and the sample to be tested in the reagent kit 211 through the lifting motor 31.

[0043] Among them, the pipetting member 32 can perform pipetting operations on multiple pools of the kit 211 through a disposable pipette tip. The lifting motor 31 is only used to drive the pipetting member 32 to move in the vertical direction, so that the pipetting member 32 can insert the pipette tip when facing the pipette tip. The lifting motor 31 is also used to drive the pipetting member 32 to descend, so that the pipette tip inserted by the pipetting member 32 can extend below the liquid level of the pool for liquid suction operation. In a possible implementation manner, in the vertical direction, the pipetting member 32 may include a first position and a second position. When the pipetting assembly 30 does not need to perform pipetting operations, the pipetting member 32 is located at the first position and on top of the kit 211; when the kit 211 moves below the pipetting member 32 and the pipette tip or the pool corresponds to the pipetting member 32, the vertical motor provides a driving force and drives the pipetting member 32 to move downward a preset number of steps in the vertical direction, so that the pipette tip can penetrate into the pool.

[0044] In the embodiment of the present application, the POCT blood cell analyzer horizontally drives the loading seat 21 through the driving motor 22 and vertically drives the pipetting member 32 through the lifting motor 31, so that the driving motor 22 and the lifting motor 31 can cooperate to complete the pipetting operation of the kit, with simple operation, compact structure, and less space occupied during motor driving, effectively reducing the instrument cost of the blood cell analyzer.

[0045] In one embodiment, the POCT blood cell analyzer further includes a chamber door 110. The chamber door 110 is arranged on the side wall of the seat body 10 and is rotatably connected to the seat body 10. The loading seat 21 is used to push the chamber door 110 under the drive of the driving motor 22, so that the chamber door 110 rotates and the loading seat 21 is partially or fully exposed outside the seat body 10.

[0046] Specifically, the seat body 10 is surrounded by side walls. The chamber door 110 can be arranged on one of the side walls. The chamber door 110 can be rotatably connected to the side wall through a rotating member. The driving motor 22 is used to drive the loading seat 21 to push the chamber door 110, and the chamber door 110 rotates and opens under the pushing force of the loading seat 21. The driving motor 22 can continue to drive the loading seat 21 to move outward until it reaches the loading and unloading working position, so that the loading seat 21 is fully exposed outside the seat body 10; or, the driving motor 22 stops working and the loading seat 21 remains in the current position, and the loading seat 21 is partially exposed outside the seat body 10 for the user to place the kit 211 on the loading seat 21 or unload the kit 211 from the loading seat 21.

[0047] In the embodiments of the present application, the POCT hematology analyzer is shielded by the storage door 110, so that the POCT hematology analyzer can perform tests in a closed environment, reducing the leakage risk of the POCT hematology analyzer; and the drive motor 22 can also be used to drive the loading seat 21 to push the storage door 110, so as to improve the utilization rate of the drive motor 22 and the drive efficiency of the POCT hematology analyzer.

[0048] In one embodiment, the loading seat 21 includes a separately provided first loading seat 210 and a second loading seat 220. The first loading seat 210 is used to receive the reagent kit 211, and the second loading seat 220 is used to receive the fluorescence test card 221.

[0049] Specifically, the first loading seat 210 and the second loading seat 220 can be integrally provided or connected by a connecting member. The first loading seat 210 is used to place the reagent kit 211, and the second loading seat 220 is used to receive the fluorescence test card 221. In an alternative embodiment, the second loading seat 220 is provided with a second groove and a pressing plate is provided above the second groove. The shape of the second groove corresponds to that of the fluorescence test card 221. The fluorescence test card 221 is placed in the second groove, and the pressing plate is used to limit the fluorescence test card 221, so that the fluorescence test card 221 is not easily moved during movement.

[0050] Optionally, please refer to Figure 3 , Figure 3 is Figure 2 the structural schematic diagram of the second loading seat in Figure 3 As shown in

[0051] the second loading seat 220 is also used to receive the biochemical test card 222. A detection window is opened at the bottom of the second loading seat 220. The POCT hematology analyzer further includes a biochemical detection component 52. The biochemical detection component 52 is arranged at the bottom of the second loading seat 220 and corresponds to the detection window, and is used to perform biochemical detection on the biochemical test card 222 in the second loading seat 220.

[0052] In an embodiment of the present application, by disposing the biochemical detection component 52 at the bottom of the second loading seat 220 and corresponding to the detection window, the biochemical detection component 52 can directly perform biochemical detection on the second loading seat 220, with high detection efficiency; moreover, the biochemical detection component 52 can be used to test enzymes, proteins, electrolytes, trace elements, etc. in a blood sample, so that the POCT blood cell analyzer of this embodiment can perform tests on multiple detection items and has a wide range of uses.

[0053] In one embodiment, the transfer component 20 includes a slide rail 230, an actuator (not shown in the figure), and a motion mechanism (not shown in the figure). The slide rail 230 is disposed on the seat body 10. The motion mechanism is slidably connected to the slide rail 230. The loading seat 21 is disposed on the motion mechanism. The actuator includes a push rod and a flange member. The driving motor 22 is connected to the push rod and is used to drive the push rod to rotate. The flange member is sleeved on the push rod and is connected to the motion mechanism. The flange member is used to receive the rotational force of the push rod and drive the motion mechanism to move in the horizontal direction.

[0054] Specifically, the loading seat 21 is installed on the motion mechanism. The motion mechanism is used to drive the loading seat 21 to move. The slide rail 230 is used to guide the motion mechanism to move in the horizontal direction. The slide rail 230 includes but is not limited to a roller guide rail and a ball guide rail. The actuator includes a push rod and a flange member. The output shaft of the driving motor 22 is connected to the push rod. The push rod is used to receive the driving force of the driving motor 22 and rotate in a preset direction. The flange member is sleeved on the push rod and is connected to the motion mechanism. The flange member is used to receive the rotational force of the push rod and drive the motion mechanism to move in the horizontal direction. In an alternative embodiment, the slide rail 230 is a ball guide rail, so that the driving motor 22 can accurately move the motion mechanism to a specified position when driving the motion mechanism.

[0055] Optionally, the motion mechanism includes a slider, a mounting block, and a side plate. The slider is slidably connected to the slide rail 230. The mounting block is fixed to the slider and is connected to the driving motor 22. The loading seat 21 is connected to the mounting block through the side plate; the loading seat 21, the side plate, and the mounting block are connected by fixing members, or the loading seat 21, the side plate, and the mounting block are integrally provided.

[0056] In one embodiment, the POCT blood cell analyzer further includes a shielding component 60. Optionally, in one embodiment, as Figure 1 shown, the shielding component 60 can be fixedly disposed on the seat body 10. The shielding component 60 forms a shielding space. When impedance detection is performed, the test kit 211 is located in the shielding space formed by the shielding component 60. The electrode connector is electrically connected to the electrode on the test kit 211. The CBC analog circuit board card is located outside the shielding space to reduce the interference of the electromagnetic signal generated by the CBC analog circuit board card on the electrical pulse signal generated during the electrical connection process and improve the accuracy of impedance detection.

[0057] Optionally, in another embodiment, the shielding component 60 may be movably or rotatably connected to the seat body 10. When the shielding component 60 rotates to a certain angle, the shielding component 60 and the side plate of the seat body 10 jointly form a shielding space, so that the kit 211 and the electrode connecting member are located in the shielding space, and the CBC analog circuit board card is located outside the shielding space, reducing the interference of the electromagnetic signal generated by the CBC analog circuit board card on the electrical pulse signal generated during the electrical connection process.

[0058] In one embodiment, when the loading seat 21 loads the kit 211 and the fluorescence detection card 221, the fluorescence detection card 221 is a detection card with the sample to be tested added. Alternatively, when the loading seat 21 loads the kit 211 and the fluorescence detection card 221, the kit 211 can be used to store the sample to be tested, and the fluorescence detection card 221 is a blank detection card that has not been used or added with the sample to be tested; the POCT hematology analyzer can also add the sample to be tested on the kit 211 to the fluorescence detection card 221 through the pipetting component 30, so as to reduce the manual operation process and improve the user experience.

[0059] Please refer to Figure 4 , Figure 4 which is a schematic flow chart of an embodiment of the hematology analysis method provided by this application. As Figure 4 shown, the hematology analysis method of this embodiment is applied to the POCT hematology analyzer in any of the above embodiments. The hematology analysis method includes the following steps:

[0060] Step S11: Move the loading seat 21 loaded with the kit 211 and the fluorescence detection card 221 to the first working position.

[0061] Specifically, the POCT hematology analyzer further includes a fourth working position and a fifth working position. When the loading seat 21 is located at the fourth working position, the user places at least one of the kit 211, the fluorescence detection card 221, and the biochemical detection card 222 on the loading seat 21. After the loading seat 21 loads the kit 211 and the fluorescence detection card 221, the POCT hematology analyzer moves the loading seat 21 to the first working position, and starts from the first working position to move the loading seat 21 to the fifth working position. The kit 211 is used to receive operations such as inserting the pipette tip, pipetting, mixing, and preparing the test solution by the pipetting component 30 at the fifth working position, so that the test solution is placed on the detection pool of the kit 211, and then continues to move the prepared kit 211 to the first working position.

[0062] Step S12: Control the loading seat 21 at the first working position to move the first number of steps, so as to move the loading seat 21 to the second working position and perform impedance detection on the kit 211.

[0063] Control the loading seat 21 at the first working position to move forward by a first number of steps, so as to move the loading seat 21 to the second working position, and perform impedance counting tests on the test solution in the detection cell through the impedance detection component 40.

[0064] In a possible implementation manner, the impedance detection component 40 can perform tests on the first detection item and the second detection item through the reagent kit 211. The driving motor 22 is used to move the reagent kit 211 to the fifth working position. The liquid transfer component 30 is used to perform liquid transfer on multiple cells at the fifth working position to prepare the first test solution. The driving motor 22 is also used to move the reagent kit 211 to the second working position, so that the impedance detection component 40 performs counting tests on the first detection item of the first test solution; the driving motor 22 is also used to continue to move the reagent kit 211 to the fifth working position. The liquid transfer member 32 is also used to perform liquid transfer on multiple cells at the fifth working position to prepare the second test solution. The driving motor 22 is also used to move the reagent kit 211 to the second working position, so that the impedance detection component 40 performs counting tests on the second detection item of the second test solution.

[0065] Further, after the impedance detection component 40 completes the first detection item and before performing the test on the second detection item, it is necessary to transfer the remaining first test solution in the detection cell to the non-detection cell through the liquid transfer member 32, so that the detection cell can continue to be used for the test of the second detection item, realizing the reuse of the detection cell. Exemplarily, the first detection item may include at least one of red blood cell count test and platelet count test, and the second detection item may include at least one of white blood cell count test and reticulocyte count test.

[0066] Step S13: Control the loading seat 21 at the second working position to return to the first working position, and control the loading seat 21 at the first working position to move by a second number of steps, so that the loading seat 21 is located at the third working position.

[0067] Specifically, after the impedance detection is completed, control the loading seat 21 to move backward by a first number of steps, so that the loading seat 21 returns from the second working position to the first working position. Control the loading seat 21 at the first working position to move by a second number of steps, so that the loading seat 21 moves from the first working position to the third working position.

[0068] Step S14: Perform fluorescence detection on the fluorescence detection card 221 at the third working position.

[0069] In response to the loading seat 21 being located at the third working position and aligning the light generator of the fluorescence detection component 51 with the top of the detection area of the fluorescence detection card 221, by gradually moving the fluorescence detection card 221 forward, the detection beam emitted by the light generator can scan the detection area of the fluorescence detection card 221 and perform fluorescence detection.

[0070] In the embodiments of the present application, the POCT blood cell analyzer first performs impedance detection on the kit 211, so that the fluorescence detection card 221 can incubate the sample during the impedance detection process, utilize the time of impedance detection for sample incubation, save the waiting time for incubation, and improve the detection speed.

[0071] Please refer to Figure 5 , Figure 5 which is a schematic structural diagram of an embodiment of the computer-readable storage medium provided by the present application. As Figure 5 shown, program instructions 111 capable of implementing all the above methods are stored in the computer-readable storage medium 11.

[0072] If the units integrated in each functional unit in the embodiments of the present application are implemented in the form of software functional units and sold or used as independent products, they can be stored in the computer-readable storage medium 11. Based on such an understanding, the technical solution of the present application, in essence, or the part that contributes to the prior art, or all or part of the technical solution, can be embodied in the form of a software product. The computer-readable storage medium 11 includes several instructions in a program instruction 111 to enable a computer device (which can be a personal computer, a system server, or a network device, etc.), an electronic device (such as an MP3, an MP4, etc., can also be a mobile terminal such as a mobile phone, a tablet computer, a wearable device, etc., or a desktop computer, etc.) or a processor to execute all or part of the steps of the methods in various embodiments of the present application.

[0073] Those skilled in the art should understand that the embodiments of the present application can be provided as a method, a system, or a computer program product. Therefore, the present application can take the form of a complete hardware embodiment, a complete software embodiment, or an embodiment combining software and hardware aspects. Moreover, the present application can take the form of a computer program product implemented on one or more computer-readable storage media 11 (including but not limited to disk storage, CD-ROM, optical storage, etc.) containing computer-usable program code.

[0074] The present application is described according to the flowcharts and / or block diagrams of the methods, devices (systems), and computer program products of the embodiments of the present application. It should be understood that each flow and / or block in the flowchart and / or block diagram, and the combination of flows and / or blocks in the flowchart and / or block diagram can be implemented by the computer-readable storage medium 11. These computer-readable storage media 11 can be provided to the processor of a general-purpose computer, a special-purpose computer, an embedded processor, or other programmable data processing devices to generate a machine, so that the program instructions 111 executed by the processor of the computer or other programmable data processing devices generate for implementing in the process Figure 1 each process or multiple processes and / or blocksFigure 1 means for the functions specified in one or more boxes.

[0075] These computer-readable storage media 11 may also be stored in a computer-readable memory that can direct a computer or other programmable data processing device to work in a particular manner, such that the program instructions 111 stored in the computer-readable storage media 11 produce a manufactured article including an instruction means that implements in the process Figure 1 one process or a plurality of processes and / or boxes Figure 1 the functions specified in one box or a plurality of boxes.

[0076] These computer-readable storage media 11 may also be loaded onto a computer or other programmable data processing device, such that a series of operation steps are performed on the computer or other programmable device to produce a computer-implemented process, so that the program instructions 111 executed on the computer or other programmable device provide steps for implementing the functions specified in Figure 1 one process or a plurality of processes and / or boxes Figure 1 one box or a plurality of boxes.

[0077] Any process or method description represented in a flowchart or otherwise described herein can be understood to represent a module, segment, or portion of code including one or more executable instructions for implementing a specific logical function or process, and the scope of the preferred embodiments of the present application includes additional implementations, where the functions may be performed in a substantially simultaneous manner or in a reverse order according to the functions involved, rather than in the order shown or discussed, which should be understood by those skilled in the technical field to which the embodiments of the present application belong.

[0078] The logic and / or steps represented in a flowchart or otherwise described herein, for example, can be considered as a sequenced list of executable instructions for implementing a logical function, and can be specifically implemented in any computer-readable medium for use by an instruction execution system, apparatus, or device (which can be a personal computer, server, network device, or other system that can fetch and execute instructions from the instruction execution system, apparatus, or device), or in conjunction with these instruction execution systems, apparatuses, or devices. The above are only embodiments of the present application, and do not limit the patent scope of the present application. Any equivalent structural or equivalent process transformation made by using the content of the specification and drawings of the present application, or directly or indirectly applied in other related technical fields, are equally included in the patent protection scope of the present application.

[0079] The above are only the embodiments of the present application, and do not limit the patent scope of the present application accordingly. Any equivalent structure or equivalent process transformation made by using the content of the specification and drawings of the present application, or directly or indirectly applied in other related technical fields, shall be similarly included in the patent protection scope of the present application.

Claims

1. A POCT blood cell analyzer, characterized in that, Comprising: A base; A transfer assembly disposed on the base, the transfer assembly including a loading base and a driving motor, the driving motor being configured to drive the loading base to move and receive a reagent kit and a fluorescence detection card; An impedance detection assembly and a fluorescence detection assembly disposed on the base, the driving motor further being configured to move the reagent kit on the loading base to the impedance detection assembly for impedance detection and move the fluorescence detection card on the loading base to the fluorescence detection assembly for fluorescence detection.

2. The POCT blood cell analyzer according to claim 1, characterized in that, The POCT blood cell analyzer further includes a sensor and a processor, the sensor being disposed on the base and configured to detect whether the loading base is at a first working position, the processor being respectively connected to the sensor and the driving motor, the processor being configured to control the driving motor to move the loading base at the first working position to a second working position so that the impedance detection assembly performs impedance detection on the reagent kit, and the processor is further configured to control the driving motor to move the loading base at the first working position to a third working position so that the fluorescence detection assembly performs fluorescence detection on the fluorescence detection card.

3. The POCT blood cell analyzer according to claim 2, wherein, The second working position and the third working position are arranged in parallel, or the second working position and the third working position are the same position.

4. The POCT blood cell analyzer according to claim 2, wherein When the loading base is at the second working position, the impedance detection assembly further includes an electrode connection member, the electrode connection member being electrically connected to the electrodes on the reagent kit; And / or, when the loading base is at the third working position, the fluorescence detection assembly further includes a light generator, and the detection light beam emitted by the light generator irradiates the detection area of the fluorescence detection card.

5. The POCT blood cell analyzer according to claim 1, characterized in that, The POCT blood cell analyzer further includes a liquid transfer assembly, the liquid transfer assembly being disposed on the base, the liquid transfer assembly including a lifting motor and a liquid transfer member, the lifting motor being configured to drive the liquid transfer member to move in the vertical direction, and the transfer assembly is further configured to move the reagent kit to below the liquid transfer member by the driving force of the driving motor so that the liquid transfer member sequentially transfers the reagents and the test samples in the reagent kit through the lifting motor.

6. The POCT blood cell analyzer according to claim 1, wherein, The POCT blood cell analyzer further includes a chamber door, the chamber door being disposed on the side wall of the base and rotatably connected to the base, the loading base being configured to push the chamber door under the drive of the driving motor so that the chamber door rotates and the loading base is partially or fully exposed from the base.

7. The POCT blood cell analyzer according to claim 1, characterized in that, The loading base includes a separately provided first loading base and a second loading base, the first loading base being configured to receive a reagent kit, and the second loading base being configured to receive a fluorescence detection card.

8. The POCT blood cell analyzer according to claim 7, wherein The second loading base is further configured to receive a biochemical detection card, a detection window is opened at the bottom of the second loading base, the POCT blood cell analyzer further includes a biochemical detection assembly, the biochemical detection assembly being disposed at the bottom of the second loading base and corresponding to the detection window, and being configured to perform biochemical detection on the biochemical detection card of the second loading base.

9. The POCT blood cell analyzer according to claim 1, wherein, The transfer component includes a slide rail, an actuator, and a motion mechanism. The slide rail is disposed on the base body. The motion mechanism is slidably connected to the slide rail. The loading seat is disposed on the motion mechanism. The actuator includes a push rod and a flange member. The driving motor is connected to the push rod and is used to drive the push rod to rotate. The flange member is sleeved on the push rod and is connected to the motion mechanism. The flange member is used to receive the rotational force of the push rod and drive the motion mechanism to move in the horizontal direction.

10. A method for blood cell analysis, characterized in that, Applied to the POCT blood cell analyzer according to any one of claims 1-9, the blood cell analysis method includes: Moving the loading seat loaded with the reagent kit and the fluorescence detection card to the first working position; Controlling the loading seat at the first working position to move a first number of steps, so as to move the loading seat to the second working position and perform impedance detection on the reagent kit; Controlling the loading seat at the second working position to return to the first working position, and controlling the loading seat at the first working position to move a second number of steps, so that the loading seat is located at the third working position; Performing fluorescence detection on the fluorescence detection card at the third working position.

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