Photoelectric resonance blood coagulation detection system and signal processing method

Abstract

The invention discloses a photoelectric resonance blood coagulation detection system and a signal processing method. The photoelectric resonance blood coagulation detection system comprises a control module, a signal processing module and a signal processing module, wherein the control module is used for controlling the working process of an instrument; the man-machine interaction module is electrically connected with the control module, and the man-machine interaction module provides a visual operation interface; the heating module is electrically connected with the control module and is used for heating the sample to be detected under the action of the control module; the vibration excitation module is electrically connected with the control module, the control module sends an excitation signal to a vibrator through the vibration excitation module, and the vibration excitation module controls the vibrator to generate variable-frequency vibration; the sample adding module is electrically connected with the control module and is used for conveying a sample to be tested to a test part of the kit through pneumatic pressure; the optical detection module is electrically connected with the control module and comprises an optical emitter and an optical detector, and the optical emitter and the optical detector are used for optically measuring the movement of the sample to be detected. The thrombelastogram detection method provided by the invention replaces a traditional thrombelastogram detection method, and has the advantages of high detection efficiency, simplicity and convenience in operation, low requirements on external environment and the like.

Description

Technical Field

[0001] The present invention relates to the field of in vitro diagnostic medical devices, and particularly to a photoelectric resonance coagulation detection system and a signal processing method. Background Art

[0002] Thromboelastogram detection has been proven to be a more advanced coagulation detection method than conventional coagulation detection. Thromboelastogram can simulate the entire process of coagulation - fibrinolysis in the human body environment, helping doctors quickly and intuitively judge the coagulation function and analyze the causes, and timely and specifically detect the coagulation function of patients.

[0003] The photoelectric resonance coagulation detection method is to quickly and accurately detect the change of the natural frequency (resonance frequency) of a blood sample after fully reacting with a reagent during the process of changing from a liquid state to a gel state and then to a solid state through the principle of optical detection. During the process of the blood sample changing from a liquid state to a gel state and then to a solid state, the amplitude value generated by resonance changes accordingly. The liquid surface amplitude value at the time of collecting the resonance frequency is collected, and a curve of the liquid surface amplitude value changing with time is drawn, and a thromboelastogram is obtained through an algorithm. Summary of the Invention

[0004] The present invention provides a photoelectric resonance coagulation detection system, including:

[0005] A sample to be tested: a blood sample mixed with a reagent;

[0006] A control module: controlling the working process of the detection system;

[0007] A human - machine interaction module: the human - machine interaction module is electrically connected to the control module, and the human - machine interaction module provides a visual operation interface;

[0008] A power supply module: electrically connected to the control module, and the power supply module provides direct current to the detection system;

[0009] A heating module: electrically connected to the control module, including left and right heating circuits, and the heating module adjusts the temperature of the sample to be tested under the action of the control module;

[0010] A vibration excitation module: electrically connected to the control module, and the control module sends an excitation signal to a vibrator through the vibration excitation module;

[0011] A sample adding module: electrically connected to the control module, and the sample adding module transfers the sample to be tested to the test position of a reagent kit through pneumatic pressure;

[0012] An optical detection module: electrically connected to the control module, including an optical emitter and an optical detector, and the optical emitter and the optical detector are used for optically measuring the movement of the sample to be tested.

[0013] Further, the vibration excitation module and the optical detection module are required to be synchronized, and the optical detection module accurately measures the motion frequency of the sample to be measured.

[0014] Further, the power supply module uses a "conventional" local voltage regulator design, which also helps to eliminate input and output power supply noise at the subsystem end.

[0015] Further, the vibration excitation module includes a high-voltage power amplifier, which can amplify the low-voltage signal to the high-voltage signal required by the vibrator.

[0016] Further, the optical detection module amplifies and converts the collected optical signal into a digital signal, and the digital signal is input into the algorithm software of the human-computer interaction module.

[0017] Further, the control module controls the heating module to adjust the sample temperature by resistive heating.

[0018] The present invention also provides a method for processing photoelectric resonance coagulation detection signals.

[0019] It includes the following steps:

[0020] Step 1: Collect the maximum amplitude value signal S1 of a single cycle of the thromboelastogram, find the resonance frequency value according to the maximum amplitude value, find the time value t1 when resonance occurs according to the resonance frequency value, and establish a coordinate system with the amplitude as the y-axis and the time as the x-axis;

[0021] Step 2: Use a digital filter (FIR) to filter and denoise the collected signal S1, and then offset the filtered signal S1 in the y-axis direction to obtain signal S2;

[0022] Step 3: Calibrate signal S2 to obtain the original thromboelastogram signal S3;

[0023] Step 4: Perform point plotting, smoothing, and mirroring processing on signal S3 to obtain the thromboelastogram S4.

[0024] The beneficial effects of the present invention: The present invention provides a photoelectric resonance coagulation detection system and a signal processing method, which have the advantages of high detection efficiency, simple operation, good stability, and low requirements for the external environment compared with the traditional thromboelastogram detection method.

[0025] The above description is only an overview of the technical solution of the present invention. In order to be able to understand the technical means of the present invention more clearly and implement it in accordance with the content of the description, the following will be described in detail with reference to the preferred embodiments of the present invention and the accompanying drawings. The specific implementation manners of the present invention are given in detail by the following embodiments and their accompanying drawings. Description of the Drawings

[0026] The accompanying drawings described herein are used to provide a further understanding of the present invention and form a part of this application. The schematic embodiments of the present invention and their descriptions are used to explain the present invention and do not constitute an improper limitation of the present invention. In the drawings:

[0027] Figure 1 is a flowchart of the optoelectronic resonance coagulation detection signal processing method according to an embodiment of the present invention;

[0028] Figure 2 is a dot plot of the amplitude signal according to an embodiment of the present invention;

[0029] Figure 3 is a dot plot of the amplitude signal after filtering and translation according to an embodiment of the present invention;

[0030] Figure 4 is the original thromboelastogram after calibration processing according to an embodiment of the present invention;

[0031] Figure 5 is the thromboelastogram after smoothing and mirror processing according to an embodiment of the present invention;

[0032] Figure 6 is a schematic diagram of the module connection of the optoelectronic resonance coagulation detection system according to an embodiment of the present invention.

[0033] In the drawings, the list of components represented by each reference numeral is as follows:

[0034] 1. Control module; 2. Human-machine interaction module; 3. Power supply module; 4. Heating module; 5. Vibration excitation module; 6. Sampling module; 7. Optical detection module; 40. Left heating circuit; 41. Right heating circuit; 50. Vibrator; 60. Kit; 70. Optical transmitter; 71. Optical detector. Detailed Embodiments

[0035] The principles and features of the present invention will be described below with reference to the accompanying drawings. The examples given are only used to explain the present invention and are not intended to limit the scope of the present invention. In the following paragraphs, the present invention will be described more specifically by way of example with reference to the accompanying drawings. The advantages and features of the present invention will be clearer according to the following description and the claims. It should be noted that the drawings are all in a very simplified form and use non-precise scales, and are only used to facilitate and clearly assist in explaining the purpose of the embodiments of the present invention.

[0036] It should be noted that when a component is referred to as "fixed to" another component, it can be directly on the other component or there can be an intermediate component. When a component is considered to be "connected to" another component, it can be directly connected to the other component or there may be an intermediate component at the same time. When a component is considered to be "disposed on" another component, it can be directly disposed on the other component or there may be an intermediate component at the same time. The terms "upper", "lower", "left", "right" and similar expressions used herein are for illustrative purposes only.

[0037] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by those of ordinary skill in the technical field to which this invention belongs. The terms used in the description of the present invention herein are for the purpose of describing specific embodiments only and are not intended to limit the present invention. The term "and / or" used herein includes any and all combinations of one or more of the related listed items.

[0038] Please refer to Figure 6 , the optoelectronic resonance coagulation detection system of the embodiment of the present invention is applied to thromboelastogram detection, and the optoelectronic resonance coagulation detection system includes:

[0039] Control module 1: Controls the working process of the instrument;

[0040] Human-machine interaction module 2: The human-machine interaction module 2 is electrically connected to the control module 1, and the human-machine interaction module 2 provides a visual operation interface;

[0041] Power supply module 3: The power supply module 3 is electrically connected to the control module 1, and the power supply module 3 provides direct current to the detection system;

[0042] Heating module 4: The heating module 4 is electrically connected to the control module 1, the heating module 4 is electrically connected to the left heating circuit 40 and the right heating circuit 41, and the control module 1 controls the heating module 4 to heat the sample solution loaded into the test kit 60 according to the sample solution temperature;

[0043] Vibration excitation module 5: The vibration excitation module is electrically connected to the control module 1, and the control module 1 sends an excitation signal to the vibrator 50 through the vibration excitation module 5;

[0044] Sampling module 6: The sampling module 6 is electrically connected to the control module 1, and the sampling module 6 transfers the sample to be tested to the test chamber of the test kit 60 through pneumatic pressure and vacuum;

[0045] Optical detection module 7: Electrically connected to the control module, including an optical transmitter and an optical detector, and the optical transmitter and the optical detector are used to optically measure the movement of the sample to be tested.

[0046] The control module 1 can specifically be a Cortex processor based on the ARM architecture. The human-computer interaction module 2 sends instructions to the control module 1, and the control module 1 executes all real-time controls of the detection system. The power module 3 can output stable direct current to supply power to the system after passing through a voltage regulator. The optical detection module 7 and the vibration excitation module 5 are signal-synchronized. The heating module 4 performs closed-loop control on the left heating circuit 40 and the right heating circuit 41 according to the temperature set value of the Cortex. The sampling module 6 uses pneumatic pressure and vacuum to drive the sample to be tested to move in the test kit 60, fully mix it with the pre-buried reagent, and drive the sample after mixing the reagent to the designated test site.

[0047] For the photoelectric resonance blood coagulation detection signal processing method, refer to Figure 1 , collect the maximum amplitude value signal S1 of a single cycle of the thromboelastogram, find the frequency value at which resonance occurs according to the maximum amplitude value, find the time value t1 at which resonance occurs according to the resonance frequency value, establish a coordinate system with the amplitude as the y-axis and the time as the x-axis, plot the amplitude values and time values of all cycles on the coordinate system to obtain the original amplitude signal plot (such as Figure 2 ), use a digital filter (FIR) to filter and denoise the collected signal S1, and then offset the filtered signal S1 in the y-axis direction to obtain the amplitude signal plot after signal filtering and translation (such as Figure 3 ), calibrate the signal S2, and obtain the original thromboelastogram curve through a mapping function (such as Figure 4 ), smooth the original thromboelastogram curve, and after mirroring it relative to the x-axis, obtain the thromboelastogram (such as Figure 5 ).

[0048] The working principle of the present invention is: through the principle of optical detection, quickly and accurately detect the change in the natural frequency (resonance frequency) of the blood sample that has fully reacted with the reagent during the process of changing from a liquid state to a gel state and then to a solid state. During the process of the blood sample changing from a liquid state to a gel state and then to a solid state, the amplitude value generated due to resonance changes accordingly. Collect the liquid surface amplitude value at the resonance frequency, depict the curve of the liquid surface amplitude value changing with time, and obtain the thromboelastogram through an algorithm.

[0049] As described above, it is only the preferred embodiment of the present invention, and it is not intended to limit the present invention in any form; any ordinary technician in the industry can smoothly implement the present invention according to the illustrations in the specification and the above description; however, any equivalent changes such as slight modifications, decorations, and evolutions made by those skilled in the art without departing from the technical solution of the present invention and using the technical content disclosed above are all equivalent embodiments of the present invention; at the same time, any equivalent changes, modifications, and evolutions made to the above embodiments based on the essential technology of the present invention still fall within the protection scope of the technical solution of the present invention.

Claims

1. The present invention provides a photoelectric resonance coagulation detection system, comprising: Sample to be tested: blood sample after mixing with reagent; Control module: controls the workflow of the detection system; Human-computer interaction module: the human-computer interaction module is electrically connected to the control module, and the human-computer interaction module provides a visual operation interface; A power module: electrically connected to the control module, the power module provides direct current to the detection system; a heating module: electrically connected to the control module, the heating module adjusts the temperature of the sample to be tested under the action of the control module; Vibration excitation module: electrically connected to the control module, the control module sends an excitation signal to the vibrator through the vibration excitation module, and the vibration excitation module controls the vibrator to generate vibrations of variable frequency; Sample adding module: electrically connected to the control module, the sample adding module transfers the sample to be tested to the test site of the test kit through pneumatic pressure; Optical detection module: electrically connected to the control module, comprising an optical emitter and an optical detector, wherein the optical emitter and the optical detector are used to optically measure the movement of the sample to be tested.

2. Furthermore, the vibration excitation module and the optical detection module are required to be synchronized, and the optical detection module accurately measures the amplitude value of the sample to be tested.

3. Further, the power module can be designed using a "conventional" local voltage regulator, which also helps to eliminate input and output power noise at the subsystem end.

4. Furthermore, the vibration excitation module includes a high-voltage power amplifier, which can amplify the low-voltage signal to the high-voltage signal required by the vibrator.

5. Furthermore, the optical detection module amplifies the collected light signal and converts it into a digital signal, and the digital signal is input into the algorithm software of the human-computer interaction module.

6. Furthermore, the control module controls the heating module to adjust the temperature of the sample to be tested by resistive heating.

7. Furthermore, the heating module includes left and right heating circuits, and the left and right heating circuits are placed on both sides of the test site of the test kit.

8. The present invention also provides a method for processing photoelectric resonance coagulation detection signals. The steps include: Step 1: collect the maximum amplitude signal S1 of a single cycle of the thromboelastogram, find out the resonant frequency value according to the maximum amplitude value, find out the time value t1 of the resonance occurrence according to the resonant frequency value, and establish a coordinate system with amplitude as the y-axis and time as the x-axis; Step 2: Use a digital filter (FIR) to filter and remove noise from the collected signal S1, and then offset the filtered signal S1 in the y-axis direction to obtain a signal S2; Step 3: calibrate the signal S2 to obtain the original thromboelastogram signal S3; Step 4: perform point mapping, smoothing, and mirroring processing on the signal S3 to obtain the thromboelastogram S4.

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