Controlled release data analysis method of flavone-doped active film

Abstract

The invention particularly relates to a controlled release data analysis method of a flavone-doped active film. The method comprises the following steps: preparing materials; acquiring data; analyzing data; and evaluating the result. According to the method, in the data analysis link, the conformity is calculated by comprehensively considering multiple factors such as satisfaction, adjacent deviation and intra-group average deviation in the modes of chart establishment, grouping analysis and the like, and then the expected value is obtained. Meanwhile, analyzing the residual flavone content in the film from the aspects of residual rate, area average difference value, different area ratio and the like when the flavone release time is up to obtain a correction value, and finally synthesizing the expected value and the correction value to obtain an evaluation value; the comprehensive and meticulous data processing mode can more accurately reflect the controlled release performance of the flavone active film, and provides a reliable basis for film performance evaluation.

Description

Technical Field

[0001] The present invention relates to the field of pharmaceutical technology, and particularly to a method for analyzing the controlled release data of a flavone-active film. Background Art

[0002] Flavonoids have various biological activities. As a new type of drug carrier, the flavone-active film can achieve the controlled release of flavones, improving the efficacy and safety of drugs.

[0003] However, at present, there is a lack of effective analysis methods for the controlled release performance data of flavone-active films. Traditional analysis methods often only focus on the overall release rate, ignoring the uneven distribution of flavones inside the film and the dynamic changes during the release process, resulting in the inability to accurately evaluate the controlled release performance of the film and making it difficult to optimize the film formulation and preparation process.

[0004] Therefore, a method for analyzing the controlled release data of a flavone-active film is needed. By analyzing the controlled release data of flavones in the flavone-active film, it is determined whether the flavone-active film meets the expected requirements to address the above-mentioned problems. Summary of the Invention

[0005] The purpose of the present invention is to provide a method for analyzing the controlled release data of a flavone-active film to solve the above problems.

[0006] To achieve the above purpose, the present invention adopts the following technical solutions: A method for analyzing the controlled release data of a flavone-active film includes the following parts: Material preparation: Prepare a preset number of flavone-active films with a set weight gradient and configure the controlled release environment according to the standard. Data acquisition: After preprocessing the flavone-active films of each weight, place them in the corresponding controlled release environment simulating the gastric space; collect the reaction solution in the controlled release environment at a preset time interval, extract the flavone content in the reaction solution and record it. Data analysis: Analyze the flavone content data in the reaction solution at each time point for the flavone-active films of each weight to obtain the controlled release expected values of the flavone-active films of each weight; and analyze the remaining flavone content in the flavone-active film when the flavone release time is reached according to the flavone release time in the expected requirements to obtain a correction value; comprehensively combine the expected value and the correction value to obtain an evaluation value. Result evaluation: Evaluate the flavone-active film based on the evaluation value obtained from the analysis.

[0007] Preferably, the analysis of the flavone content data in the reaction solution obtained at each time point for the flavone-active films of each weight specifically includes the following parts: Taking time as the abscissa and the flavonoid content in the reaction solution as the ordinate, a graph is established. The flavonoid content in the reaction solution obtained at each time point is marked on the graph and denoted as a numerical point; According to the time series, all numerical points are divided into several groups with a preset quantity, and the numerical points within each group are different from each other; After analyzing the numerical points within each group and comparing them with the expected requirements, the compliance of the numerical points within each group with the expected requirements is obtained; and the compliances corresponding to the numerical points within each group are comprehensively processed to obtain the controlled release expected value of the flavonoid active film with the corresponding weight.

[0008] Preferably, the analysis of the numerical points within each group and the comparison with the expected requirements to obtain the compliance of the numerical points within each group with the expected requirements specifically includes the following parts: From the expected requirements, the expected flavonoid content of the components released during the time corresponding to the numerical points within the group is obtained, and the flavonoid content corresponding to the numerical points within the group is accumulated to obtain the actual flavonoid content of the components; the actual flavonoid content of the components is divided by the expected flavonoid content of the components to obtain the satisfaction degree; According to the time series, the difference between adjacent numerical points within the group is calculated in sequence to obtain the adjacent value difference, and the number of adjacent value differences consistent with the number of numerical points is obtained. The adjacent value differences are summed to obtain the adjacent value combined difference; and the expected adjacent value combined difference in the expected requirements is obtained accordingly. After calculating the difference between the adjacent value combined difference within the group and the expected adjacent value combined difference, the absolute value is taken to obtain the adjacent deviation; The absolute values of the adjacent value differences within the group obtained above are averaged to obtain the average value within the group, and the expected average value within the group in the expected requirements is obtained. After calculating the difference between the average value within the group and the expected average value within the group, the absolute value is taken to obtain the average difference within the group; The weight factors of the satisfaction degree, adjacent deviation, and average difference within the group are preset, and the satisfaction degree, adjacent deviation, and average difference within the group are respectively multiplied by their corresponding weight factors and then summed to obtain the compliance; Thus, the compliance of each group corresponding to each weight of the flavonoid active film is obtained.

[0009] Preferably, the comprehensive processing of the compliances corresponding to the numerical points within each group to obtain the expected value of the flavonoid active film with the corresponding weight and the expected requirements specifically includes the following parts: According to the importance degree of the expected requirements, corresponding weight factors are assigned to each group in the time series, and the compliances of each group are multiplied by their corresponding weight factors and then summed to obtain the expected value of the flavonoid active film with the corresponding weight.

[0010] Preferably, according to the flavonoid release time in the expected requirements, the remaining flavonoid content in the flavonoid active film when the flavonoid release time is reached is analyzed to obtain a correction value, which specifically includes the following parts: Obtain the remaining flavonoid content in the flavonoid-active film, and divide the remaining flavonoid content by the initial flavonoid content of the flavonoid-active film to obtain the residual rate; According to the size of the flavonoid-active film, divide it into a preset number of regions, and sequentially obtain the flavonoid content of each region; divide the remaining flavonoid content in the flavonoid-active film by the number of regions to obtain the regional average value, and sequentially calculate the difference between the flavonoid content of each region and the regional average value, and take the absolute value to obtain the regional average difference; Sequentially obtain the difference in the flavonoid content contained in the adjacent regions of the flavonoid-active film, and record it as the adjacent-region difference; preset the allowable range of the adjacent-region difference, mark the adjacent-region differences that do not fall within the allowable range of the adjacent-region difference as abnormal adjacent-region differences, and mark the adjacent flavonoid-active film regions corresponding to the abnormal adjacent-region differences, count all the marked flavonoid-active film regions, and divide the number of all the marked flavonoid-active film regions by the number of regions divided by the flavonoid-active film to obtain the different-region ratio; After normalizing the residual rate, the regional average difference, and the different-region ratio, use the values of the residual rate, the regional average difference, and the different-region ratio as the three side lengths of a triangle respectively to establish a triangle model; and use the area of this triangle model as the correction value.

[0011] Preferably, the process of comprehensively obtaining the evaluation value by combining the expected value and the correction value specifically includes the following parts: After normalizing the expected value and the correction value, use the expected value as the two right-angled sides of a right-angled triangle, connect the other side to obtain a complete right-angled triangle, and use the correction value as the height of the right-angled triangle to establish a triangular pyramid model, and use the volume of this triangular pyramid model as the evaluation value.

[0012] Preferably, the process of evaluating the ketone-active film based on the obtained evaluation value specifically includes the following parts: Arrange the evaluation values corresponding to the flavonoid-active films of each weight in ascending order according to the numerical values, and select the minimum evaluation value; use the flavonoid-active film corresponding to the minimum evaluation value as the flavonoid-active film that most meets the expected requirements.

[0013] Preferably, before obtaining the remaining flavonoid content in the flavonoid-active film, first perform pre-treatment on the flavonoid-active film, specifically including: soaking the flavonoid-active film in physiological saline of a preset concentration for a predetermined time, and using a set instrument to transfer the flavonoid-active film to the area for flavonoid content determination.

[0014] In summary, due to the adoption of the above technical solutions, the beneficial effects of the present invention are: 1. In the data analysis stage of the present invention, by establishing charts, group analysis, etc., multiple factors such as satisfaction, adjacent deviation, and within-group average difference are comprehensively considered to calculate the compliance, and then the expected value is obtained. At the same time, the remaining flavonoid content in the film at the flavonoid release time is analyzed from aspects such as the residual rate, regional average difference value, and inter-region ratio to obtain the correction value. Finally, the expected value and the correction value are combined to obtain the evaluation value. This comprehensive and detailed data processing method can more accurately reflect the controlled release performance of the flavonoid active film and provide a reliable basis for film performance evaluation.

[0015] 2. In the material preparation and controlled release environment setting stages of the present invention, strict control is carried out on various experimental conditions. These strict condition controls ensure the stability and consistency of the experimental environment, making the experimental results more reliable and repeatable, and laying a solid foundation for accurate subsequent data analysis. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] In the following description of exemplary embodiments with reference to the accompanying drawings, more details, features, and advantages of the present application are disclosed. In the drawings: Figure 1 is a flowchart of the present invention; DETAILED DESCRIPTION OF THE EMBODIMENTS

[0017] The following will describe several embodiments of the present application in more detail with reference to the accompanying drawings so that those skilled in the art can implement the present application. The present application can be embodied in many different forms and for many different purposes and should not be limited to the embodiments described herein. These embodiments are provided so that the present application is thorough and complete and fully conveys the scope of the present application to those skilled in the art. The embodiments do not limit the present application.

[0018] Unless otherwise defined, all terms (including technical terms and scientific terms) used herein have the same meaning as commonly understood by those of ordinary skill in the art to which the present application belongs. It will be further understood that terms such as those defined in commonly used dictionaries should be interpreted as having a meaning consistent with their meaning in the relevant art and / or the context of this specification and will not be interpreted in an idealized or overly formal sense unless clearly so defined herein.

[0019] Please refer to Figure 1 as shown, the present invention provides a technical solution: A method for analyzing the controlled release data of a flavonoid-active film, comprising the following parts: Material preparation; preparing a preset number of flavonoid-active films with a set weight gradient and configuring the controlled release environment according to the standard; Controlled-release environment: Prepare simulated gastric juice according to relevant standards (such as the Chinese Pharmacopoeia); generally, it is made by mixing hydrochloric acid solution (concentration about 0.1mol / L) and pepsin (concentration about 10g / L); use a pH meter to precisely adjust the pH value of the simulated gastric juice to between 1.2 - 1.5 to simulate the acidic environment of the human stomach; Select appropriate glass containers, such as stoppered conical flasks; ensure that the containers are clean and dry, and perform high-temperature sterilization before use to eliminate interfering factors such as microorganisms. The size of the container should be selected according to the scale of the experiment. For example, when conducting small-dose experiments, a 100mL stoppered conical flask can be selected; if a larger reaction system is required, a 500mL or 1000mL stoppered conical flask can be chosen; Prepare magnetic stirrers, dialysis bags (the cut-off molecular weight is selected according to experimental requirements, generally 1000 - 10000Da), thermostatic magnetic stirrers, pH test papers, pH meter calibration buffers, pipettes and matching tips, filter papers, centrifuge tubes, etc.; Calibrate the pH meter using standard buffers with pH values of 4.00, 7.00, and 9.18 to ensure the accuracy of the measurement results; during the calibration process, according to the operating instructions of the pH meter, immerse the electrode into the buffers with different pH values in sequence, and perform calibration adjustments after the readings are stable; Set the temperature to 37 ± 0.5°C, which is the approximate temperature of the human stomach; turn on the stirrer and check whether the stirring speed is uniform. The stirring speed can be set to 50 - 100 revolutions per minute by adjusting the speed knob of the stirrer to simulate the stirring effect of gastric peristalsis; Obtain data: After pre-treating flavonoid active films of various weights, place them in the controlled-release environment of their respective corresponding simulated gastric spaces; collect the reaction solution in the controlled-release environment at preset time intervals, extract the flavonoid content in the reaction solution and record it; The pre-treatment of the flavonoid active film includes: putting it into a petri dish containing a small amount of normal saline and soaking for 5 - 10 minutes to make it swell initially, and at the same time, impurities that may exist on the film surface can be removed; after soaking, gently blot the water on the film surface with filter paper; After each collection of the reaction solution in the controlled-release environment, replenish an equal amount of fresh simulated gastric juice into the controlled-release environment; Data analysis: Analyze the flavonoid content data in the reaction solution at each time point for flavonoid active films of various weights to obtain the controlled-release expected values of flavonoid active films of various weights; and based on the flavonoid release time in the expected requirements, analyze the remaining flavonoid content in the flavonoid active film when the flavonoid release time is reached to obtain a correction value; comprehensively combine the expected value and the correction value to obtain an evaluation value; Analyze the flavonoid content data in the reaction solution obtained at each time point for flavonoid active films of various weights, which specifically includes the following parts: Taking time as the abscissa and the flavonoid content in the reaction solution as the ordinate, a graph is established. The flavonoid content in the reaction solution obtained at each time point is marked on the graph and recorded as a numerical point; According to the time series, all numerical points are divided into several groups with a preset quantity, and the numerical points within each group are different from each other; After analyzing the numerical points within each group and comparing them with the expected requirements, the compliance of the numerical points within each group with the expected requirements is obtained; and the compliances corresponding to the numerical points within each group are comprehensively processed to obtain the controlled release expectation value of the flavonoid active film with the corresponding weight; After analyzing the numerical points within each group and comparing them with the expected requirements, the compliance of the numerical points within each group with the expected requirements is obtained, which specifically includes the following parts: From the expected requirements, the expected flavonoid content of the components released during the time corresponding to the numerical points within the group is obtained, and the flavonoid content corresponding to the numerical points within the group is accumulated to obtain the actual flavonoid content of the components; the actual flavonoid content of the components is divided by the expected flavonoid content of the components to obtain the satisfaction degree; According to the time series, the difference between adjacent numerical points within the group is calculated in sequence to obtain the adjacent value difference, and the number of adjacent value differences consistent with the number of numerical points is obtained. The sum of each adjacent value difference is obtained to get the adjacent value combined difference; and the expected adjacent value combined difference in the expected requirements is obtained accordingly. After calculating the difference between the adjacent value combined difference within the group and the expected adjacent value combined difference, the absolute value is taken to obtain the adjacent deviation; After taking the absolute value of the adjacent value difference within the group obtained above and calculating the mean value, the within-group mean value is obtained, and the expected within-group mean value in the expected requirements is obtained. After calculating the difference between the within-group mean value and the expected within-group mean value, the absolute value is taken to obtain the within-group mean difference; Preset the weight factors of the satisfaction degree, adjacent deviation, and within-group mean difference. Multiply the satisfaction degree, adjacent deviation, and within-group mean difference by their corresponding weight factors respectively and then sum them up to obtain the compliance; Thus, the compliance of each group corresponding to each weight of the flavonoid active film is obtained; The compliances corresponding to the numerical points within each group are comprehensively processed to obtain the expectation value of the flavonoid active film with the corresponding weight and the expected requirements, which specifically includes the following parts: According to the importance degree of the expected requirements, each group on the time series is given a corresponding weight factor. The compliance of each group is multiplied by its corresponding weight factor and then summed up to obtain the expectation value of the flavonoid active film with the corresponding weight; According to the flavonoid release time in the expected requirements, when the flavonoid release time is reached, the remaining flavonoid content in the flavonoid active film is analyzed to obtain a correction value, which specifically includes the following parts: Before obtaining the remaining flavonoid content in the flavonoid-active film, the flavonoid-active film is pre-treated, specifically including: soaking the flavonoid-active film in physiological saline with a preset concentration for a predetermined time, and transferring the flavonoid-active film to the area for flavonoid content determination using a set instrument; wherein the concentration of the physiological saline is 0.9%, the predetermined soaking time is 8 minutes, and the set instrument is a sterile forceps; Obtain the remaining flavonoid content in the flavonoid-active film, and divide the remaining flavonoid content by the initial flavonoid content of the flavonoid-active film to obtain the residual rate; According to the size of the flavonoid-active film, it is divided into a preset number of areas, and the flavonoid content of each area is obtained in turn; divide the remaining flavonoid content in the flavonoid-active film by the number of areas to obtain the area average value, and calculate the difference between the flavonoid content of each area and the area average value in turn, and take the absolute value to obtain the area mean difference; Obtain the difference in the flavonoid content contained in the adjacent areas of the flavonoid-active film in turn, and record it as the adjacent area difference; preset the allowable range of the adjacent area difference, mark the adjacent area differences that do not fall within the allowable range of the adjacent area difference as abnormal adjacent area differences, and mark the adjacent flavonoid-active film areas corresponding to the abnormal adjacent area differences, count all the marked flavonoid-active film areas, and divide the number of all the marked flavonoid-active film areas by the number of areas divided by the flavonoid-active film to obtain the abnormal area ratio; After normalizing the residual rate, the area mean difference, and the abnormal area ratio, use the values of the residual rate, the area mean difference, and the abnormal area ratio as the three side lengths of a triangle respectively to establish a triangle model; and use the area of this triangle model as the correction value.

[0020] Among them, the selection of the triangle area model is based on the three-dimensional synergistic effect of the residual rate, the area mean difference, and the abnormal area ratio: the residual rate reflects the total amount control, the area mean difference reflects the spatial uniformity, and the abnormal area ratio quantifies the defect degree; the geometric combination of the three can intuitively reflect the overall performance of the film; Integrate the expected value and the correction value to obtain the evaluation value, which specifically includes the following parts: After normalizing the expected value and the correction value, use the expected value as the two right-angled sides of a right-angled triangle, connect the other side to obtain a complete right-angled triangle, and establish a triangular pyramid model with the correction value as the height of the right-angled triangle, and use the volume of this triangular pyramid model as the evaluation value; Among them, the triangular pyramid volume model combines the expected value (two-dimensional plane) and the correction value (height) to realize the four-dimensional comprehensive evaluation of the time dimension (expected value) and the space dimension (correction value); Result evaluation: Evaluate the ketone-active film based on the obtained evaluation value; Evaluate the ketone-active film based on the obtained evaluation value, which specifically includes the following parts: Arrange the evaluation values corresponding to the flavonoid active films of each weight in ascending order according to the numerical values, and select the smallest evaluation value among them; use the flavonoid active film with the weight corresponding to the smallest evaluation value as the flavonoid active film that most meets the expected requirements.

[0021] The above description of the embodiments enables those skilled in the art to implement or use the present invention. Various modifications to these embodiments will be obvious to those skilled in the art, and the general principles defined herein can be implemented in other embodiments without departing from the spirit or scope of the present invention. Therefore, the present invention will not be limited to these embodiments shown herein, but rather to the widest scope consistent with the principles and novel features disclosed herein.

Claims

1. A data analysis method for controlled release of a flavone-doped active film, characterized in that, It includes the following parts: Material preparation; Prepare a preset number of flavonoid active films with a set weight gradient, and configure a controlled release environment according to the standard; Obtain data: After preprocessing the flavonoid active films of each weight, place them in the controlled release environment corresponding to their respective simulated gastric spaces; Collect the reaction solution in the controlled release environment at preset time intervals, extract the flavonoid content in the reaction solution and record it; Data analysis: Analyze the flavonoid content data in the reaction solution at each time point for the flavonoid active films of each weight to obtain the controlled release expected values of the flavonoid active films of each weight; And according to the flavonoid release time in the expected requirements, analyze the remaining flavonoid content in the flavonoid active film when the flavonoid release time is reached to obtain a correction value; Combine the expected value and the correction value to obtain an evaluation value; Result evaluation: Evaluate the ketone active film according to the evaluation value obtained from the analysis.

2. The controlled release data analysis method of a flavone-doped active film according to claim 1, wherein The analysis of the flavonoid content data in the reaction solution obtained at each time point for the flavonoid active films of each weight specifically includes the following parts: Establish a graph with time as the abscissa and the flavonoid content in the reaction solution as the ordinate, mark the flavonoid content in the reaction solution obtained at each time point on the graph, and record it as a numerical point; According to the time series, divide all the numerical points into several groups with a preset number, and the numerical points in each group are different; After analyzing the numerical points in each group and comparing them with the expected requirements, obtain the compliance of the numerical points in each group with the expected requirements; And comprehensively process the compliance corresponding to the numerical points in each group to obtain the controlled release expected value of the flavonoid active film of the corresponding weight.

3. A method for analyzing the controlled release data of a flavonoid-doped active film according to claim 2, characterized in that, The analysis of the numerical points in each group and comparing them with the expected requirements to obtain the compliance of the numerical points in each group with the expected requirements specifically includes the following parts: Obtain the expected component flavonoid content released within the time corresponding to the numerical points in the group from the expected requirements, accumulate the flavonoid content corresponding to the numerical points in the group to obtain the actual component flavonoid content; Divide the actual component flavonoid content by the expected component flavonoid content to obtain the satisfaction; According to the time series, calculate the difference between adjacent numerical points in the group in turn to obtain the adjacent value difference, obtain the number of adjacent value differences consistent with the number of numerical points, sum up each adjacent value difference to obtain the adjacent value combined difference; And obtain the expected adjacent value combined difference in the expected requirements accordingly, calculate the difference between the adjacent value combined difference in the group and the expected adjacent value combined difference, and take the absolute value to obtain the adjacent deviation; Take the absolute value of the adjacent value difference in the group obtained above and calculate the mean value to obtain the within-group mean value, and obtain the expected within-group mean value in the expected requirements, calculate the difference between the within-group mean value and the expected within-group mean value, and take the absolute value to obtain the within-group mean difference; Preset the weight factors of satisfaction, adjacent deviation, and within-group mean difference, multiply satisfaction, adjacent deviation, and within-group mean difference by their corresponding weight factors respectively and then sum them up to obtain the compliance; Thus, obtain the compliance of each group corresponding to each weight flavonoid active film.

4. A method for analyzing the controlled release data of a flavonoid-doped active film according to claim 3, characterized in that The comprehensive processing of the compliance corresponding to the numerical points in each group to obtain the expected value of the flavonoid active film of the corresponding weight and the expected requirements specifically includes the following parts: According to the importance of the expected requirements, corresponding weight factors are assigned to each group in the time series. After multiplying the compliance of each group by its corresponding weight factor and summing them up, the expected value of the corresponding weight flavonoid-active film is obtained.

5. A method for analyzing the controlled release data of a flavonoid-doped active film according to claim 1, characterized in that, Based on the flavonoid release time in the expected requirements, when the flavonoid release time is reached, the remaining flavonoid content in the flavonoid-active film is analyzed to obtain a correction value, which specifically includes the following parts: Obtain the remaining flavonoid content in the flavonoid-active film, and divide the remaining flavonoid content by the initial flavonoid content of the flavonoid-active film to obtain the residual rate; According to the size of the flavonoid-active film, it is divided into a preset number of regions, and the flavonoid content of each region is obtained in turn; divide the remaining flavonoid content in the flavonoid-active film by the number of regions to obtain the regional average value, and calculate the difference between the flavonoid content of each region and the regional average value in turn, and take the absolute value to obtain the regional mean difference; Obtain the difference in the flavonoid content contained in the adjacent regions of the flavonoid-active film in turn, and record it as the adjacent region difference; Preset the allowable range of the adjacent region difference, mark the adjacent region differences that do not fall within the allowable range of the adjacent region difference as abnormal adjacent region differences, and mark the adjacent flavonoid-active film regions corresponding to the abnormal adjacent region differences. Count all the marked flavonoid-active film regions, and divide the number of all the marked flavonoid-active film regions by the number of regions divided by the flavonoid-active film to obtain the different region ratio; After normalizing the residual rate, the regional mean difference, and the different region ratio, use the values of the residual rate, the regional mean difference, and the different region ratio as the three side lengths of a triangle respectively to establish a triangle model; and use the area of this triangle model as the correction value.

6. A method for analyzing the controlled release data of a flavone-doped active film according to claim 1, characterized in that, The comprehensive combination of the expected value and the correction value to obtain an evaluation value specifically includes the following parts: After normalizing the expected value and the correction value, use the expected value as the two right-angled sides of a right-angled triangle, connect the other side to obtain a complete right-angled triangle, and use the correction value as the height of the right-angled triangle to establish a triangular pyramid model. Use the volume of this triangular pyramid model as the evaluation value.

7. A method for analyzing the controlled release data of a flavonoid-doped active film according to claim 1, characterized in that, Evaluating the flavonoid-active film based on the obtained evaluation value specifically includes the following parts: Arrange the evaluation values corresponding to the flavonoid-active films of each weight in ascending order according to the numerical size, and select the minimum evaluation value; use the flavonoid-active film corresponding to the minimum evaluation value as the flavonoid-active film that best meets the expected requirements.

8. A method for analyzing the controlled release data of a flavone-doped active film according to claim 5, characterized in that Before obtaining the remaining flavonoid content in the flavonoid-active film, first pre-treat the flavonoid-active film, specifically including: soaking the flavonoid-active film in physiological saline with a preset concentration for a predetermined time, and using a set instrument to transfer the flavonoid-active film to the area for flavonoid content determination.

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