Cancer detection chip based on immune marker

Abstract

The invention discloses a cancer detection chip based on an immune marker, and relates to the technical field of cancer detection chips. A circuit board is fixedly connected to the interior of the shell; a water tank is fixedly connected to one side of the circuit board in the shell; a water pump is fixedly connected into the water tank; the top end of the water pump is fixedly connected with a first connecting pipe; a circulating pipe is mounted at one end of the circuit board; the water pump pumps water into the first connecting pipe, the water enters the circulating pipe through the first connecting pipe to flow, heat of the circuit board and the chip can be transmitted and diffused to the circulating pipe, the heat is taken away through flowing of the water in the circulating pipe, and therefore heat dissipation treatment is conducted on the circuit board and the chip, and the water enters the water tank through the circulating pipe. The heat dissipation mode is arranged on the back of the circuit board, normal operation is not affected, the chip is made to operate in a proper environment for a long time, the frequency of replacing chip assemblies is reduced, and the use cost is reduced.

Description

Technical Field

[0001] The present invention relates to the technical field of cancer detection chips, and particularly relates to a cancer detection chip based on immune markers. Background Art

[0002] A cancer detection chip is an integrated detection device based on microfluidic technology, biosensing or molecular diagnostic principles, which can quickly identify cancer-related biomarkers through a small amount of body fluid, so as to realize early screening, typing or prognosis monitoring of cancer; this technology combines material science, nanotechnology, optical analysis and artificial intelligence algorithms, and has the advantages of high throughput, high sensitivity, non-invasiveness, etc., and has gradually become the core tool in the field of liquid biopsy.

[0003] Existing cancer detection chips mainly selectively capture target biomarkers through micron-level channel design combined with surface modification; fluorescence labeling, circular dichroism spectroscopy or impedance change are used to detect the target in real time.

[0004] Although the technology of cancer detection chips has developed rapidly, there are still problems in its practical application. Local high temperature is easily generated during the operation of the chip, resulting in the denaturation and inactivation of biomolecules and affecting the detection accuracy; existing solutions need to rely on external cooling devices for rapid cooling, increasing the system complexity and cost; the performance attenuation during long-term use, the microfluidic channels are prone to efficiency decline due to biological contamination or nanoscale structure wear, and the chip components need to be frequently replaced, increasing the use cost. Summary of the Invention

[0005] The purpose of the present invention is to provide a cancer detection chip based on immune markers, so as to solve the technical problem that in the prior art, the microfluidic channels are prone to efficiency decline due to biological contamination or nanoscale structure wear, and the chip components need to be frequently replaced, increasing the use cost.

[0006] The technical problem to be solved by the present invention can be realized through the following technical solutions:

[0007] A cancer detection chip based on immune markers includes a housing; a circuit board is fixedly connected inside the housing; a water tank is fixedly connected to one side of the circuit board inside the housing; a water pump is fixedly connected inside the water tank; a first connecting pipe is fixedly connected to the top of the water pump; a circulation pipe is installed at one end of the circuit board, and one side of the circulation pipe is connected to the first connecting pipe; the other end of the circulation pipe is fixedly connected to a second connecting pipe; a chip connecting plate is fixedly connected inside the circuit board.

[0008] As a further scheme of the present invention: an ice maker is fixedly connected inside the housing; an electric slide rail is fixedly connected inside the ice maker; a motor is slidably connected to the top of the electric slide rail; an ice making box is fixedly connected to the output end of the motor.

[0009] As a further solution of the present invention: a first cylinder is fixedly connected to the side end of the ice maker; a second cylinder is fixedly connected to the side end of the first cylinder; a vibration block is fixedly connected to the bottom end of the second cylinder, and the vibration block is arranged above the ice making box.

[0010] As a further solution of the present invention: a limiting frame is fixedly connected to the inner side wall of the water tank; a limiting elastic piece is fixedly connected to the inner side wall of the limiting frame; a rubber elastic rod is fixedly connected to the inner side wall of the limiting frame, and one end of the rubber elastic rod is connected to the limiting elastic piece.

[0011] As a further solution of the present invention: a first rotating rod is rotatably connected to the bottom end of the water pump; a spiral blade is fixedly connected to the bottom end of the first rotating rod; a second rotating rod is rotatably connected to the inside of the water tank; a belt is rotatably connected between the second rotating rod and the first rotating rod; a stirring blade is fixedly connected to the bottom end of the second rotating rod.

[0012] As a further solution of the present invention: a crushing rod is fixedly connected to the top end of the second rotating rod; the crushing rod is arranged below the ice maker.

[0013] As a further solution of the present invention: one end of the circulation pipe is connected to a heat dissipation pipe; a heat absorption plate is fixedly connected to one end of the chip connection board, and the heat dissipation pipe is arranged inside the heat absorption plate; a heat absorption fin is fixedly connected to one end of the heat dissipation pipe; a heat collection fin is fixedly connected to the inside of the heat dissipation pipe; a heat dissipation ring is fixedly connected to the bottom end of the heat collection fin.

[0014] As a further solution of the present invention: a sealing strip is fixedly connected to the inside of the ice maker, and the sealing strip is arranged above the ice making box.

[0015] The beneficial effects of the present invention: The water pump pumps water into the first connection pipe, and the water flows into the inside of the circulation pipe through the first connection pipe. The heat of the circuit board and the chip will be transferred and diffused to the circulation pipe, and the flowing water inside the circulation pipe takes away the heat, thereby cooling the circuit board and the chip. Then the water enters the inside of the water tank through the circulation pipe, avoiding the influence of high temperature on the denaturation and inactivation of biomolecules and ensuring the detection accuracy. This heat dissipation method is arranged on the back of the circuit board without affecting the normal operation, enabling the chip to operate in a suitable environment for a long time, reducing the number of times of replacing chip components, and reducing the use cost. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] The following further describes the present invention with reference to the accompanying drawings.

[0017] Figure 1 It is a schematic diagram of the overall structure of the present invention;

[0018] Figure 2 It is a schematic diagram of the housing structure of the present invention;

[0019] Figure 3 It is a schematic diagram of the circuit board structure of the present invention;

[0020] Figure 4 is the present invention Figure 2 The enlarged view of part A in the present invention;

[0021] Figure 5 is the schematic structural view of the heat absorption plate in the present invention;

[0022] In the figure: 1. housing; 2. circuit board; 3. water tank; 4. water pump; 5. first connecting pipe; 6. circulation pipe; 7. chip connecting plate; 8. second connecting pipe; 9. heat dissipation pipe; 10. heat absorption plate; 11. heat absorption fin; 12. heat collection fin; 13. heat dissipation ring; 14. first rotating rod; 15. spiral blade; 16. belt; 17. second rotating rod; 18. stirring blade; 19. ice maker; 20. electric slide rail; 21. motor; 22. ice making box; 23. first air cylinder; 24. second air cylinder; 25. vibration block; 26. limiting frame; 27. limiting elastic sheet; 28. rubber elastic rod; 29. crushing rod. Specific embodiments

[0023] Next, the technical solutions in the embodiments of the present invention will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without making creative efforts belong to the scope of protection of the present invention.

[0024] As Figures 1-5 shown, a cancer detection chip based on immune markers includes a housing 1; a circuit board 2 is fixedly connected inside the housing 1; a water tank 3 is fixedly connected to one side of the circuit board 2 inside the housing 1; a water pump 4 is fixedly connected inside the water tank 3; a first connecting pipe 5 is fixedly connected to the top of the water pump 4; a circulation pipe 6 is installed at one end of the circuit board 2, and one side of the circulation pipe 6 is connected to the first connecting pipe 5; the other end of the circulation pipe 6 is fixedly connected to a second connecting pipe 8; a chip connecting plate 7 is fixedly connected inside the circuit board 2.

[0025] During operation, the circulation pipe 6 is installed on the back of the circuit board 2, and the chip connection board 7 is arranged at the bottom of the chip. Heat is generated during the operation of the chip. At this time, the water pump 4 is started. There is water stored inside the water tank 3. The water pump 4 pumps the water into the first connecting pipe 5. The water enters the inside of the circulation pipe 6 through the first connecting pipe 5 and flows. The heat of the circuit board 2 and the chip will be transferred and diffused to the circulation pipe 6. The flowing water inside the circulation pipe 6 takes away the heat, thereby dissipating the heat of the circuit board 2 and the chip. The water then enters the inside of the water tank 3 through the circulation pipe 6, avoiding the influence of high temperature on the denaturation and inactivation of biomolecules and ensuring the detection accuracy. This heat dissipation method is set on the back of the circuit board 2 without affecting the normal operation, enabling the chip to operate in a suitable environment for a long time, reducing the number of times of replacing chip components, and reducing the use cost;

[0026] A ice maker 19 is fixedly connected inside the housing 1; an electric slide rail 20 is fixedly connected inside the ice maker 19; a motor 21 is slidably connected to the top end of the electric slide rail 20; an ice making box 22 is fixedly connected to the output end of the motor 21.

[0027] If the temperature is high in summer and the ordinary water cooling is insufficient, the motor 21 is slid by the electric slide rail 20. The sliding of the motor 21 causes the ice making box 22 to slide out of the inside of the ice maker 19. The motor 21 rotates the ice making box 22. There are ice cubes inside the ice making box 22. After the motor 21 rotates the ice making box 22, the ice cubes fall into the inside of the water tank 3, which can cool the water inside the water tank 3, thereby improving the cooling effect;

[0028] A first cylinder 23 is fixedly connected to the side end of the ice maker 19; a second cylinder 24 is fixedly connected to the side end of the first cylinder 23; a vibration block 25 is fixedly connected to the bottom end of the second cylinder 24, and the vibration block 25 is arranged above the ice making box 22.

[0029] The second cylinder 24 can repeatedly make the vibration block 25 hit the ice making box 22 to generate vibration. The repeated hitting of the ice making box 22 by the vibration block 25 can make the ice cubes fall, preventing the ice cubes from getting stuck inside the ice making box 22. The first cylinder 23 can adjust the position of the second cylinder 24;

[0030] A limiting frame 26 is fixedly connected to the inner side wall of the water tank 3; a limiting elastic sheet 27 is fixedly connected to the inner side wall of the limiting frame 26; a rubber elastic rod 28 is fixedly connected to the inner side wall of the limiting frame 26, and one end of the rubber elastic rod 28 is connected to the limiting elastic sheet 27.

[0031] The limiting frame 26 can limit the ice cubes to prevent the ice cubes from affecting the normal rotation of the stirring blade 18. The limiting frame 26 is provided with holes and grooves inside to facilitate the contact between water and ice cubes and prevent the ice cubes from escaping from the limiting frame 26. When the ice cubes fall, the limiting spring piece 27 will bend. When the stirring blade 18 rotates to stir the water, the ice cubes will move with the water. The limiting spring piece 27 can limit the ice cubes. The rubber elastic rod 28 can support the position of the limiting spring piece 27 to ensure that the limiting spring piece 27 limits the ice cubes.

[0032] The bottom end of the water pump 4 is rotatably connected to a No. 1 rotating rod 14; the bottom end of the No. 1 rotating rod 14 is fixedly connected to a spiral blade 15; the inside of the water tank 3 is rotatably connected to a No. 2 rotating rod 17; a belt 16 is rotatably connected between the No. 2 rotating rod 17 and the No. 1 rotating rod 14; the bottom end of the No. 2 rotating rod 17 is fixedly connected to a stirring blade 18.

[0033] Water will enter from the bottom end of the water pump 4. The water will drive the spiral blade 15 to rotate during the flow. The rotation of the spiral blade 15 will rotate the belt 16. The rotation of the belt 16 will rotate the second rotating rod 17. The rotation of the second rotating rod 17 can rotate the stirring blade 18. The stirring blade 18 and the spiral blade 15 can cooperate to stir the water inside the water tank 3, and the cold water around the ice cubes can be mixed with other water. The rotation of the stirring blade 18 can accelerate the melting speed of the ice cubes, so that the low temperature of the ice cubes is mixed with water, and the circuit board 2 can be quickly cooled.

[0034] A crushing rod 29 is fixedly connected to the top end of the second rotating rod 17 ; the crushing rod 29 is arranged below the ice maker 19 .

[0035] The rotation of the stirring blade 18 causes the crushing rod 29 to rotate, and the rotation of the crushing rod 29 can impact the ice cubes in the limiting frame 26. After the crushing rod 29 impacts and crushes the ice cubes, the mixing of the ice cubes and water can be accelerated, so that the water temperature inside the water tank 3 can be quickly reduced, and the heat dissipation effect on the circuit board 2 can be improved;

[0036] One end of the circulation pipe 6 is connected to a heat dissipation pipe 9; one end of the chip connection plate 7 is fixedly connected to a heat absorption plate 10, and the heat dissipation pipe 9 is arranged inside the heat absorption plate 10; one end of the heat dissipation pipe 9 is fixedly connected to a heat absorption sheet 11; the inside of the heat dissipation pipe 9 is fixedly connected to a heat collecting sheet 12; the bottom end of the heat collecting sheet 12 is fixedly connected to a heat dissipation ring 13.

[0037] The chip connection board 7 is provided at the back of the chip. The heat of the chip will be transferred to the chip connection board 7. The heat absorption plate 10 and the heat absorption fin 11 are made of heat absorption materials. The heat absorption plate 10 can quickly absorb the heat of the chip connection board 7 and transfer and diffuse it to the heat absorption plate 10. The heat absorption plate 10 then transfers and diffuses the heat to the heat absorption fin 11. The heat absorption fin 11 transfers and diffuses the heat to the heat collection fin 12 and the heat dissipation ring 13. The heat dissipation pipe 9 is connected to the circulation pipe 6. The water inside the circulation pipe 6 will pass through the inside of the heat dissipation pipe 9. When the water passes through the heat dissipation ring 13, it will take away the heat, so as to quickly dissipate the heat of the heat absorption plate 10, and then quickly dissipate the heat of the chip;

[0038] A sealing strip is fixedly connected inside the ice maker 19, and the sealing strip is provided above the ice making box 22.

[0039] The ice maker 19 can perform ice making treatment repeatedly to reduce the leakage of low temperature inside the ice maker 19. The sealing strip can improve the sealing performance of contact with the ice making box 22, so as to reduce energy leakage and reduce energy consumption;

[0040] Working principle of the present invention: During operation, the circulation pipe 6 is installed on the back of the circuit board 2, and the chip connection plate 7 is arranged at the bottom of the chip. Heat is generated during the operation of the chip. At this time, the water pump 4 is started. Water is stored inside the water tank 3. The water pump 4 pumps water into the first connecting pipe 5. The water enters the inside of the circulation pipe 6 through the first connecting pipe 5 and flows. The heat of the circuit board 2 and the chip will be transferred and diffused to the circulation pipe 6. The flowing water inside the circulation pipe 6 takes away the heat, thereby dissipating heat from the circuit board 2 and the chip. Then the water enters the inside of the water tank 3 through the circulation pipe 6, avoiding the influence of high temperature on the denaturation and inactivation of biomolecules and ensuring the detection accuracy. This heat dissipation method is set on the back of the circuit board 2 without affecting normal operation, enabling the chip to operate in a suitable environment for a long time, reducing the number of times of replacing chip components, and reducing the use cost; the chip connection plate 7 is arranged at the back of the chip. The heat of the chip will be transferred to the chip connection plate 7. The heat absorption plate 10 and the heat absorption fins 11 are made of heat-absorbing materials. The heat absorption plate 10 can quickly absorb the heat of the chip connection plate 7 and transfer and diffuse it to the heat absorption plate 10. The heat absorption plate 10 then transfers and diffuses the heat to the heat absorption fins 11. The heat absorption fins 11 transfer and diffuse the heat to the heat collection fins 12 and the heat dissipation ring 13. The heat dissipation pipe 9 is connected to the circulation pipe 6. The water inside the circulation pipe 6 will pass through the inside of the heat dissipation pipe 9. When the water passes through the heat dissipation ring 13, it will take away the heat, thereby quickly dissipating heat from the heat absorption plate 10 and then quickly dissipating heat from the chip; if the temperature is high in summer and ordinary water cooling is insufficient, the motor 21 is slid by the electric slide rail 20. The sliding of the motor 21 causes the ice-making box 22 to slide out of the ice maker 19. The motor 21 rotates the ice-making box 22. There are ice cubes inside the ice-making box 22. After the motor 21 rotates the ice-making box 22, the ice cubes fall into the inside of the water tank 3, which can cool the water inside the water tank 3, thereby improving the cooling effect; the second air cylinder 24 can repeatedly make the vibration block 25 impact the ice-making box 22 to cause vibration. By repeatedly impacting the ice-making box 22 with the vibration block 25, the ice cubes can fall, preventing the ice cubes from getting stuck inside the ice-making box 22. The first air cylinder 23 can adjust the position of the second air cylinder 24; the ice maker 19 can repeatedly perform ice-making treatment, reducing the leakage of low temperature inside the ice maker 19. The sealing strip can improve the sealing performance of the contact with the ice-making box 22, thereby reducing energy leakage and lowering energy consumption; water enters from the bottom end of the water pump 4. During the flowing process of the water, it drives the spiral blade 15 to rotate. The rotation of the spiral blade 15 causes the belt 16 to rotate. The rotation of the belt 16 further causes the second rotating rod 17 to rotate. The rotation of the second rotating rod 17 can rotate the stirring blade 18. The cooperation of the rotation of the stirring blade 18 and the spiral blade 15 stirs the water inside the water tank 3, mixing the cold water around the ice cubes with other water. Moreover, the rotation of the stirring blade 18 can accelerate the melting speed of the ice cubes, mixing the low temperature of the ice cubes with the water and quickly dissipating heat from the circuit board 2;The limiting frame 26 can limit the ice cubes to prevent the ice cubes from affecting the normal rotation of the stirring blade 18. The limiting frame 26 is provided with holes and grooves inside to facilitate the contact between water and ice cubes and prevent the ice cubes from escaping from the limiting frame 26. When the ice cubes fall, the limiting spring piece 27 will bend. When the stirring blade 18 rotates to stir the water, the ice cubes will move with the water. The limiting spring piece 27 can limit the ice cubes. The rubber elastic rod 28 can support the position of the limiting spring piece 27 to ensure that the limiting spring piece 27 limits the ice cubes. The rotation of the stirring blade 18 causes the crushing rod 29 to rotate. The rotation of the crushing rod 29 can impact the ice cubes in the limiting frame 26. After the crushing rod 29 impacts and crushes the ice cubes, the mixing of the ice cubes and water can be accelerated, thereby quickly reducing the water temperature inside the water tank 3 and improving the heat dissipation effect on the circuit board 2. ;

[0041] The above is a detailed description of an embodiment of the present invention, but the content is only a preferred embodiment of the present invention and cannot be considered to limit the scope of implementation of the present invention. All equivalent changes and improvements made within the scope of the present invention should still fall within the scope of the patent coverage of the present invention.

Claims

1. A cancer detection chip based on immune markers, characterized in that: The invention comprises a shell (1); a circuit board (2) is fixedly connected to the inside of the shell (1); a water tank (3) is fixedly connected to one side of the circuit board (2) inside the shell (1); a water pump (4) is fixedly connected to the inside of the water tank (3); a first connecting pipe (5) is fixedly connected to the top of the water pump (4); a circulation pipe (6) is installed at one end of the circuit board (2), and one side of the circulation pipe (6) is connected to the first connecting pipe (5); a second connecting pipe (8) is fixedly connected to the other end of the circulation pipe (6); and a chip connecting plate (7) is fixedly connected to the inside of the circuit board (2).

2. The cancer detection chip based on immune markers according to claim 1, characterized in that: An ice maker (19) is fixedly connected inside the housing (1); an electric slide rail (20) is fixedly connected inside the ice maker (19); a motor (21) is slidably connected to the top end of the electric slide rail (20); and an ice box (22) is fixedly connected to the output end of the motor (21).

3. The cancer detection chip based on immune markers according to claim 2, characterized in that: The side end of the ice maker (19) is fixedly connected to a No. 1 cylinder (23); the side end of the No. 1 cylinder (23) is fixedly connected to a No. 2 cylinder (24); the bottom end of the No. 2 cylinder (24) is fixedly connected to a vibration block (25), and the vibration block (25) is arranged above the ice box (22).

4. The cancer detection chip based on immune markers according to claim 1, characterized in that: The inner wall of the water tank (3) is fixedly connected to a limit frame (26); the inner wall of the limit frame (26) is fixedly connected to a limit spring sheet (27); the inner wall of the limit frame (26) is fixedly connected to a rubber elastic rod (28), and one end of the rubber elastic rod (28) is connected to the limit spring sheet (27).

5. The cancer detection chip based on immune markers according to claim 1, characterized in that: The bottom end of the water pump (4) is rotatably connected to a first rotating rod (14); the bottom end of the first rotating rod (14) is fixedly connected to a spiral blade (15); the inside of the water tank (3) is rotatably connected to a second rotating rod (17); a belt (16) is rotatably connected between the second rotating rod (17) and the first rotating rod (14); and the bottom end of the second rotating rod (17) is fixedly connected to a stirring blade (18).

6. The cancer detection chip based on immune markers according to claim 5, characterized in that: A crushing rod (29) is fixedly connected to the top end of the second rotating rod (17); the crushing rod (29) is arranged below the ice maker (19).

7. The cancer detection chip based on immune markers according to claim 1, characterized in that: One end of the circulation pipe (6) is connected to a heat dissipation pipe (9); one end of the chip connection plate (7) is fixedly connected to a heat absorption plate (10), and the heat dissipation pipe (9) is arranged inside the heat absorption plate (10); one end of the heat dissipation pipe (9) is fixedly connected to a heat absorption sheet (11); the inside of the heat dissipation pipe (9) is fixedly connected to a heat collection sheet (12); and the bottom end of the heat collection sheet (12) is fixedly connected to a heat dissipation ring (13).

8. The cancer detection chip based on immune markers according to claim 2, characterized in that: A sealing strip is fixedly connected to the interior of the ice maker (19), and the sealing strip is arranged above the ice box (22).

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