Application of prodigiosin in promoting germination of plant seeds

Abstract

According to the application of prodigiosin in promoting germination of the plant seeds, a prodigiosin ethanol solution is applied to germination of the plant seeds, and the GI value of the plant seeds can be remarkably increased; wherein the germination promoting effect on tobacco seeds is optimal, and the highest GI value reaches 203.32%; after summer radish seeds are treated, the highest GI value reaches 154.49%, after arabidopsis thaliana seeds are treated, the highest GI value reaches 142.20%, and after pakchoi seeds are treated, the highest GI value reaches 142.60%.

Description

Technical Field

[0001] The present invention relates to the technical field of bio-agriculture, and particularly relates to the application of prodigiosin in promoting the germination of plant seeds. Background Art

[0002] During the process of crop cultivation, there have always been problems such as large amounts of chemical fertilizers and pesticides being applied, which have caused serious non-point source pollution to the soil and the environment, and to a certain extent, have affected the process of green and sustainable development of agriculture. With the continuous enhancement of people's awareness of environmental protection and health care, new natural plant growth regulators have been increasingly emphasized.

[0003] Prodigiosin is a photosensitive pigment with a tripyrrole ring skeleton structure, which can be metabolically synthesized by some microorganisms such as the genus Serratia ( Serratia ), the genus Streptomyces ( treptomyces ), the genus Pseudomonas ( seudomonas ), and some marine bacteria. Prodigiosin has the abilities of antibacterial, antiviral, antitumor, algicidal and alga-inhibiting, insecticidal, etc., and has broad application prospects in the fields of medicine, food preservation, biological pesticides, pollution control, etc.

[0004] In the application document with the application number CN202411663729.7 and the title of a composite film for fruit preservation, its preparation method and application, a composite film with prodigiosin and a film-forming matrix (chitosan combined with corn starch or polyvinyl alcohol) as the core components is disclosed. Experimental data show that this composite film has remarkable effects in inhibiting microorganisms and delaying the deterioration of fruit quality; in the composite film with corn starch as the matrix, when the prodigiosin concentration is 1×10⁻³ wt%, the inhibition zone diameters against Escherichia coli and Staphylococcus aureus reach 8.90 mm and 8.57 mm respectively, and at the same time, the water vapor transmission rate is as low as 0.15 g·m / m²·Pa·s, the light transmittance exceeds 40% in the visible light range, and it can significantly reduce the weight loss rate and decay rate of strawberries, and delay the decrease of vitamin C content; in the composite film with polyvinyl alcohol as the matrix, when the prodigiosin concentration is 1 mg / L, the water permeability rate is as low as 0.1121 g·cm² / d, and the fresh-keeping effect on bananas is better than that of commercially available PE film, the weight loss rate is only 4.34% (on the 10th day), and the retention amount of vitamin C content reaches 2.631 mg / 100 g; this composite film has the functions of antibacterial, water and oxygen barrier, and antioxidant, can effectively extend the storage period of fruits, and the raw materials are safe and degradable, with practical application potential.

[0005] In the patent application with the application number CN201610534232.4 and the subject name of a dyeing method for silk fabrics using prodigiosin, a dyeing method that optimizes the strain cultivation process and adds Elymus nutans extract is disclosed, which significantly improves the dyeing effect and antibacterial properties. Experimental data shows that under the conditions of only 0.1 - 0.5% of the prodigiosin dosage based on the silk quality, a dyeing temperature of 60 - 70 °C, and a heat preservation time of 15 - 25 min, the soaping color fastness, dry rubbing fastness, and wet rubbing fastness of the dyed fabric all reach level 4, which is better than the traditional method. The addition of Elymus nutans extract significantly enhances the antibacterial effect. The antibacterial rates against Candida albicans, Escherichia coli, and Staphylococcus aureus reach 98%, 95%, and 97% respectively, which is significantly improved compared with the control group without the added extract. This method does not require a mordant, the dyeing efficiency is increased by 4 times, and at the same time, the energy consumption and the use of chemical reagents are reduced, combining environmental protection and functionality, providing a new solution for the application of natural dyes in the field of silk dyeing.

[0006] In the patent application with the application number CN202410232947.9 and the subject name of an organometallic framework nanomaterial doped with copper and prodigiosin, its preparation method and application, an organometallic framework nanomaterial ZCProP doped with copper and prodigiosin is disclosed, which is prepared by doping copper and prodigiosin in zeolitic imidazolate framework - 90 by a one - pot method and modifying with polyethylene glycol. Experimental data shows that the material has uniform size, uniform distribution of copper elements, successful embedding of prodigiosin, and the surface modification with polyethylene glycol significantly reduces the zeta potential and improves biocompatibility. ZCProP has dual responsiveness to ATP and low pH, and has a significant degradation rate at pH 4.5 and in the presence of ATP, and can target mitochondria (the coincidence rate with mitochondrial markers shown by laser confocal microscopy reaches 0.79). In the experiment on tumor - bearing mice, the tumor volume growth inhibition effect of the ZCProP treatment group is the best within 14 days, shrinking by about 70% compared with the control group. This material generates free radicals through copper - ion - induced cuproptosis, the synergistic effect of prodigiosin, and the Fenton - like reaction, significantly enhancing the killing effect on tumor cells while reducing systemic toxicity, providing a new strategy for the treatment of triple - negative breast cancer.

[0007] It can be seen that the application potential of prodigiosin has been gradually developed, but so far, there is no report on the application of prodigiosin in promoting plant growth. Summary of the Invention

[0008] Aiming at the above - mentioned deficiencies of the prior art, the present invention provides an application of prodigiosin in promoting the germination of plant seeds. Applying the prodigiosin ethanol solution in the germination of plant seeds can significantly increase the GI value of plant seeds. Among them, when the weight ratio of prodigiosin to ethanol in the prodigiosin ethanol solution is 1:200, the germination index (GI) of tobacco seeds is 203.32%.

[0009] The technical solution of the present invention is as follows: The application of prodigiosin in promoting the germination of plant seeds can significantly increase the GI value of plant seeds, and the GI value is as high as 203.32%.

[0010] Preferably, the above application is specifically the application of a prodigiosin ethanol solution in seed germination.

[0011] Preferably, in the prodigiosin ethanol solution, the weight ratio of prodigiosin to ethanol is 1:100 - 500; wherein, the ethanol is anhydrous ethanol.

[0012] Preferably, the above application process is as follows: (1) Add a prodigiosin ethanol solution to a petri dish containing plant seeds, and the prodigiosin ethanol solution just covers the seeds; (2) Incubate. Under a closed state, incubate at a controlled temperature for 4 - 5 days and observe the germination status.

[0013] Preferably, the plant seeds are pre - sterilized with 75% ethanol for 5 - 7 minutes, then soaked in sterile deionized water for 24 hours, and the large and plump seeds that sink to the bottom are screened.

[0014] Preferably, the plant seeds are water radish seeds, tobacco seeds, Arabidopsis seeds, and Chinese cabbage seeds.

[0015] Preferably, in step (2), the incubation is carried out under dark conditions, and the incubation temperature is 30 ± 0.5 °C.

[0016] Compared with the prior art, the beneficial effects of the present invention are as follows: In the present invention, it is first proposed that prodigiosin can promote the germination of plant seeds; among them, the effect of promoting germination on tobacco seeds is the best, and the highest GI value reaches 203.32%; after treating water radish seeds, the highest GI value reaches 154.49%, after treating Arabidopsis seeds, the highest GI value reaches 142.20%, and after treating Chinese cabbage seeds, the highest GI value reaches 142.60%. Brief Description of the Drawings

[0017] In order to more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the following will briefly introduce the drawings required for use in the description of the embodiments or the prior art. Obviously, for those of ordinary skill in the art, without creative efforts, other drawings can also be obtained based on these drawings.

[0018] Figure 1 It shows the influence of prodigiosin ethanol solutions with different concentrations on the GI value of water radish seeds.

[0019] Figure 2Effect of prodigiosin ethanol solutions with different concentrations on GI value of tobacco seeds.

[0020] Figure 3 Effect of prodigiosin ethanol solutions with different concentrations on GI value of Arabidopsis thaliana seeds.

[0021] Figure 4 Effect of prodigiosin ethanol solutions with different concentrations on GI value of Chinese cabbage seeds. Detailed implementation manners

[0022] The various exemplary implementation manners of the present invention will now be described in detail. This detailed description should not be considered as a limitation of the present invention, but rather as a more detailed description of certain aspects, characteristics and implementation manners of the present invention.

[0023] It should be understood that the terms described in the present invention are only for describing specific implementation manners and are not used to limit the present invention. Additionally, for the numerical ranges in the present invention, it should be understood that each intermediate value between the upper and lower limits of the range is also specifically disclosed. Any intermediate value within any stated value or stated range, as well as each smaller range between any other stated value or intermediate value within the stated range, is also included in the present invention. The upper and lower limits of these smaller ranges can be independently included or excluded from the range.

[0024] Unless otherwise specified, all technical and scientific terms used herein have the same meaning as commonly understood by those of ordinary skill in the art to which the present invention pertains. Although the present invention only describes preferred methods and materials, any methods and materials similar or equivalent to those described herein can also be used in the implementation or testing of the present invention. All documents mentioned in this specification are incorporated by reference to disclose and describe the methods and / or materials related to the documents. In case of conflict with any incorporated document, the content of this specification shall prevail.

[0025] Without departing from the scope or spirit of the present invention, various improvements and changes can be made to the specific implementation manners of the specification of the present invention, which are obvious to those skilled in the art. Other implementation manners obtained from the specification of the present invention are obvious to those skilled in the art. The specification and examples of the present invention are only exemplary.

[0026] Regarding the terms "comprising", "including", "having", "containing", etc. used herein, they are all open-ended terms, meaning including but not limited to.

[0027] In the present invention, the prodigiosin used is a commercially available prodigiosin standard product, 50 µg / bottle, with a purity of ≥95%, from Shanghai Yuanye Bio-Technology Co., Ltd.; the absolute ethanol is of analytical grade.

[0028] The GI value of plant seeds is calculated according to the following formula (1):

[0029] Wherein, A1 is the percentage of the number of germinated seeds in the experimental group to the total number of seeds put in, with the unit of %; A2 is the average root length value of all seeds in the experimental group, with the unit of mm; B1 is the percentage of the number of germinated seeds in the control group to the total number of seeds put in, with the unit of %; B2 is the average root length value of all seeds in the control group, with the unit of mm.

[0030] Example 1 The application of prodigiosin in promoting the germination of plant seeds is as follows: Step 1, prepare a prodigiosin ethanol solution Dissolve prodigiosin in ethanol. The weight ratio of prodigiosin to ethanol is 1:100. After mixing, a prodigiosin ethanol solution is obtained; Step 2, pre-treat plant seeds Pre-treat radish seeds, tobacco seeds, Arabidopsis seeds and Chinese cabbage seeds with 75% ethanol for 5 min respectively, and then soak the seeds in sterile deionized water for 24 h. Select the large and plump seeds that sink to the bottom; Step 3, prepare samples Experimental group: Use a petri dish with a diameter of 10 cm. Put 30 plant seeds in each petri dish, add the prodigiosin ethanol solution to the petri dish so that the prodigiosin ethanol solution covers the seeds, and record the volume; Control group: Use sterile deionized water to replace the prodigiosin ethanol solution, and the addition amount of sterile deionized water is the same as that of the prodigiosin ethanol solution; Both the experimental group and the control group are set with 3 replicates; Step 4, culture Culture radish seeds, tobacco seeds, Arabidopsis seeds and Chinese cabbage seeds under closed and dark conditions; the culture temperature is 30 ± 0.5 °C, culture for 4 d, observe the germination status, measure the root length with a vernier caliper, and calculate the GI value of plant seeds; the GI value is calculated according to Formula 1.

[0031] Example 2 The application of prodigiosin in promoting the germination of plant seeds is as follows: Step 1, prepare a prodigiosin ethanol solution Dissolve prodigiosin in ethanol. The weight ratio of prodigiosin to ethanol is 1:200. After mixing, a prodigiosin ethanol solution is obtained; Step 2, pre-treat plant seeds Respectively, pre-treat the water radish seeds, tobacco seeds, Arabidopsis seeds and pakchoi seeds with 75% ethanol for 5 min, and then soak the seeds in sterile deionized water for 24 h. Screen for large and plump seeds that sink to the bottom. Step 3: Sample preparation Experimental group: Use a petri dish with a diameter of 10 cm. Place 30 plant seeds in each petri dish. Add the prodigiosin ethanol solution to the petri dish so that the seeds are just covered, and record the volume. Control group: Use sterile deionized water to replace the prodigiosin ethanol solution, and the amount of sterile deionized water added is the same as that of the prodigiosin ethanol solution. Both the experimental group and the control group are set with 3 replicates. Step 4: Cultivation Cultivate the water radish seeds, tobacco seeds, Arabidopsis seeds and pakchoi seeds under closed and dark conditions; the cultivation temperature is 30 ± 0.5 °C, cultivate for 4 d, observe the germination status, measure the root length with a vernier caliper, and calculate the GI value of the plant seeds; the GI value is calculated according to Formula 1.

[0032] Example 3 The application of prodigiosin in promoting the germination of plant seeds is as follows: Step 1: Prepare the prodigiosin ethanol solution Dissolve prodigiosin in ethanol, and the weight ratio of prodigiosin to ethanol is 1:400. After mixing, obtain the prodigiosin ethanol solution. Step 2: Pre-treatment of plant seeds Respectively, pre-treat the water radish seeds, tobacco seeds, Arabidopsis seeds and pakchoi seeds with 75% ethanol for 5 min, and then soak the seeds in sterile deionized water for 24 h. Screen for large and plump seeds that sink to the bottom. Step 3: Sample preparation Experimental group: Use a petri dish with a diameter of 10 cm. Place 30 seeds in each petri dish. Add the prodigiosin ethanol solution to the petri dish so that the seeds are just covered, and record the volume. Control group: Use sterile deionized water to replace the prodigiosin ethanol solution, and the amount of sterile deionized water added is the same as that of the prodigiosin ethanol solution. Both the experimental group and the control group are set with 3 replicates. Step 4: Cultivation Cultivate the water radish seeds, tobacco seeds, Arabidopsis seeds and pakchoi seeds under closed and dark conditions; the cultivation temperature is 30 ± 0.5 °C, cultivate for 4 d, observe the germination status, measure the root length with a vernier caliper, and calculate the GI value of the plant seeds; the GI value is calculated according to Formula 1.

[0033] Example 4 The application of prodigiosin in promoting the germination of plant seeds is as follows: Step 1: Prepare a prodigiosin ethanol solution Dissolve prodigiosin in ethanol. The weight ratio of prodigiosin to ethanol is 1:500. After mixing, a prodigiosin ethanol solution is obtained; Step 2: Pretreat plant seeds Pre-sterilize radish seeds, tobacco seeds, Arabidopsis seeds and pakchoi seeds with 75% ethanol for 5 min respectively, and then soak the seeds in sterile deionized water for 24 h. Select the large and plump seeds that sink to the bottom; Step 3: Prepare samples Experimental group: Use a petri dish with a diameter of 10 cm. Put 30 seeds in each petri dish, add the prodigiosin ethanol solution to the petri dish so that the prodigiosin ethanol solution covers the seeds, and record the volume; Control group: Use sterile deionized water to replace the prodigiosin ethanol solution, and the amount of sterile deionized water added is the same as the amount of prodigiosin ethanol solution added; Both the experimental group and the control group are set with 3 replicates; Step 4: Cultivate Cultivate radish seeds, tobacco seeds, Arabidopsis seeds and pakchoi seeds under closed and dark conditions; the cultivation temperature is 30 ± 0.5 °C, cultivate for 4 d, observe the germination status, measure the root length with a vernier caliper, and calculate the GI value of the plant seeds; the GI value is calculated according to Formula 1.

[0034] Statistically analyze the GI values of the radish seeds, tobacco seeds, Arabidopsis seeds and pakchoi seeds cultured in Examples 1-4. The results are shown in Table 1 as follows: Table 1 Effects of prodigiosin ethanol solution on the GI value of plant seeds

[0035] Combined with Table 1, it can be seen that: ① Prodigiosin can promote the germination of radish seeds, tobacco seeds, Arabidopsis seeds and pakchoi seeds and improve the germination index of the seeds; and the germination-promoting concentration of prodigiosin for various seeds is different; ② When the weight ratio of prodigiosin to ethanol in the prodigiosin ethanol solution is 1:200, the germination indices of tobacco seeds and Arabidopsis seeds are the highest. Among them, the germination index (GI) of tobacco seeds is 203.32%, and the germination index (GI) of Arabidopsis seeds is 142.20%; ③ When the weight ratio of prodigiosin to ethanol in the prodigiosin ethanol solution is 1:400, the germination indices of radish seeds and pakchoi seeds are the highest. Among them, the germination index (GI) of radish seeds is 154.49%, and the germination index (GI) of pakchoi seeds is 142.60%; That is to say, the prodigiosin ethanol solution at a certain concentration can significantly increase the GI values of water radish seeds, tobacco seeds, Arabidopsis seeds, and pakchoi seeds.

[0036] Although the present invention has been described in detail by referring to the preferred embodiments, the present invention is not limited thereto. Without departing from the spirit and essence of the present invention, those of ordinary skill in the art can make various equivalent modifications or substitutions to the embodiments of the present invention, and these modifications or substitutions should all be within the scope of the present invention. / Any person skilled in the art within the technical scope disclosed by the present invention can easily think of changes or substitutions, which should all be covered within the protection scope of the present invention. Therefore, the protection scope of the present invention shall be subject to the protection scope of the claims.

Claims

1. Application of prodigiosin in promoting germination of plant seeds, characterized in that, It can significantly increase the GI value of plant seeds, and the GI value is as high as 203.32%.

2. The application according to claim 1, characterized in that Specifically, it is the application of prodigiosin ethanol solution in seed germination.

3. The application according to claim 2, wherein In the prodigiosin ethanol solution, the weight ratio of prodigiosin to ethanol is 1:100 - 500; among them, the ethanol is anhydrous ethanol.

4. The application according to claim 2, wherein The above application process is as follows: (1) Add the prodigiosin ethanol solution to the petri dish containing plant seeds, and the prodigiosin ethanol solution just needs to cover the seeds. (2) Incubate. Under a closed state, incubate at a controlled temperature for 4 - 5 days and observe the germination status.

5. The application according to claim 4, characterized in that The plant seeds are pre - sterilized with 75% ethanol for 5 - 7 minutes, and then soaked in sterile deionized water for 24 hours, and the large and plump seeds that sink to the bottom are screened.

6. The application according to claim 1, characterized in that, The plant seeds are water radish seeds, tobacco seeds, Arabidopsis seeds, and pakchoi seeds.

7. The application according to claim 4, characterized in that, In step (2), the incubation is carried out under dark conditions, and the incubation temperature is 30 ± 0.5 °C.

Images