Green control method for tobacco bacterial wilt

Abstract

The invention discloses a green prevention and control method for tobacco bacterial wilt. The method comprises the steps of S1, tobacco rotation, wherein two kinds of crops are planted on the same land in turn; s2, seedling raising stage prevention and treatment: adding a microbial agent into a seedling raising substrate; s3, prevention and treatment in a transplanting stage: tobacco planting soil conservation, disease-free strong seedling selection, root dipping treatment with a microbial preparation, and hole application of an organic fertilizer with a disease inhibition function and an antagonistic microbial agent; s4, prevention and control at a field management stage: respectively performing intertillage and hilling 20-25 days after transplanting, and additionally applying a microbial agent; the method has the characteristics that different prevention and control methods are adopted for the bacterial wilt in different growth stages of the tobacco, and the best prevention and control effect is achieved by comprehensively applying the synergistic effect of various prevention and control technologies; the microbial agent is added into the seedling culture substrate, so that bacteria in the early stage of disease attack can be inhibited, and the growth promoting effect on non-infected plants is achieved.

Description

Technical Field

[0001] The present invention belongs to the fields of tobacco planting and plant protection, and particularly relates to a green control method for tobacco bacterial wilt. Background Art

[0002] Tobacco bacterial wilt is a bacterial soil-borne disease caused by Ralstonia solanacearum, which can parasitize more than 50 families and hundreds of plants, causing serious losses to the production of many crops and cash crops. Under suitable conditions, the wilt bacteria can survive in the soil for more than 5 months. The bacteria infect from the wounds or natural cracks of the plant roots, first colonize in the intercellular spaces of the roots, and finally enter the xylem vessels, rapidly reproduce and spread, and spread upward through the xylem to the stem, secreting extracellular polysaccharides and other substances to cause vascular blockage, resulting in plant wilting, seriously threatening crop production.

[0003] Tobacco bacterial wilt is a typical soil-borne disease. Due to long-term continuous cropping, the control difficulty of flue-cured tobacco soil-borne diseases increases and the control cost increases. At present, the control of tobacco bacterial wilt mainly relies on chemical control. Although chemical control has a good control effect on tobacco bacterial wilt, it will increase the drug resistance of pathogenic bacteria, affect the quality of the tobacco field soil environment and pesticide residues and many other problems. Therefore, how to reasonably and effectively alleviate the continuous cropping obstacle of flue-cured tobacco, improve the soil microecological environment, thereby control the occurrence of tobacco bacterial wilt, reduce the dependence on chemical pesticide control, and ensure the healthy, sustainable and safe development of tobacco leaf production has become an important problem to be solved urgently at present. Summary of the Invention

[0004] The purpose of the present invention is to provide a green control method for tobacco bacterial wilt to solve the problems raised in the above background art.

[0005] To achieve the above purpose, the present invention provides the following technical solution: A green control method for tobacco bacterial wilt, comprising:

[0006] S1. Tobacco rotation: Rotate and plant two crops on the same piece of land;

[0007] S2. Control at the seedling stage: Add microbial inoculants to the seedling substrate;

[0008] S3. Control at the transplanting stage: Conserve the tobacco-planting soil, select disease-free and strong seedlings, dip the roots with a microbial preparation, and at the same time apply organic fertilizer with disease-suppressing function and antagonistic microbial inoculants by hole application;

[0009] S4. Prevention and control during the field management stage: respectively, conduct intertillage and soil heaping 20 - 25 days after transplanting, and apply microbial inoculants; 35 - 40 days after transplanting, conduct secondary high soil heaping on the tobacco rhizosphere, and at the same time irrigate the roots with 42% trichloroisocyanuric acid solution; 7 - 10 days after the secondary high soil heaping, alternately use Zhongshengmycin and Tilmicosin agents; during the topping stage to the harvesting stage, promptly pull out and destroy diseased plants, and disinfect with lime water to avoid the spread of the disease.

[0010] Preferably, the two crops for tobacco rotation in S1 are flue - cured tobacco - maize.

[0011] Preferably, in S2, before tobacco seeding, conduct substrate soil mixing with bacteria treatment or bacteria - treated seedling treatment, and mix the substrate soil with the fermented bacterial liquid in a ratio of 20:1 and then sow and raise seedlings.

[0012] Preferably, for the conservation of tobacco - growing soil, select oyster shell powder products with a particle size of 100 mesh (0.15 mm), evenly spread them on the tobacco field before plowing, and then plow to mix them evenly with the soil, or apply them in furrows according to the situation;

[0013] When the pH is between 4.5 and 5.0, the application rate is 100 kg / 666.7 m2;

[0014] When the pH is between 5.0 and 5.5, the application rate is 65 kg / 666.7 m2;

[0015] When the pH is between 5.5 and 5.9, the application rate is 30 kg / 666.7 m2;

[0016] Or 20 - 30 days before transplanting, deeply plow, and spread 50 - 100 kg of quicklime per 666.7 m2.

[0017] Preferably, the organic fertilizer with disease - suppressing function and antagonistic microbial inoculants are composed of the organic fertilizer used for tobacco and Bacillus amyloliquefaciens and Lysobacter, and the specific method is:

[0018] Before applying the organic fertilizer, mix the microbial inoculant with the organic fertilizer at a ratio of 50 kg / t. If the amount is small and difficult to mix evenly, first mix 50 - 100 kg of organic fertilizer with 50 kg of the microbial agent and then evenly mix it with 1 t of organic fertilizer, and apply it as a base fertilizer when making ridges in the tobacco field.

[0019] Preferably, the application rate of the organic fertilizer with disease - suppressing function and antagonistic microbial inoculants is to apply 60 - 120 kg of biological bacterial fertilizer by hole application when transplanting tobacco seedlings, and the application frequency is 1 time.

[0020] Preferably, the microbial preparation is a 150 - 250 - fold liquid of Bacillus amyloliquefaciens and Lysobacter, and the time for root soaking treatment is 10 - 30 min.

[0021] Preferably, in S4:

[0022] Carry out intertillage and soil banking 20d - 25d after transplanting, and apply microbial inoculant agricultural phage + Bacillus subtilis at 150 - 300 times dilution and Bacillus amyloliquefaciens and Lysobacter at 150 - 250 times dilution, with the dosage of 300 mL / plant - 500 mL / plant;

[0023] Preferably, in S4, 35d - 40d after transplanting, carry out secondary high soil banking on the tobacco rhizosphere, and at the same time, carry out root irrigation treatment with 800 - 1000 times dilution of 42% trichloroisocyanuric acid, with the dosage of 300 mL / plant - 500 mL / plant.

[0024] Preferably, in S4, 7d - 10d after secondary high soil banking, alternately carry out root irrigation treatment with Zhongshengmycin and Tilmicosin at 800 - 1000 times dilution, with the dosage of 300 mL / plant - 500 mL / plant. During the topping period to the harvesting period, promptly pull out and destroy the diseased plants at the disease center, and disinfect the soil around the diseased plants with 1% lime water to prevent the spread of the disease.

[0025] Compared with the prior art, the beneficial effects of the present invention are:

[0026] The characteristics of the present invention are that different prevention and control methods are adopted for bacterial wilt disease at different growth stages of tobacco, and various prevention and control technologies are comprehensively applied and synergistically act to achieve the best prevention and control effect;

[0027] The present invention adds microbial inoculant to the seedling-raising substrate, which can inhibit the germs in the initial stage of the disease and promote the growth of non-infected plants;

[0028] In view of the local soil acidification situation, the present invention selects soil acidification improvement materials such as oyster shell powder and quicklime for treatment, inhibits the breeding and reproduction of bacterial wilt germs, reduces the number of bacterial wilt germs, and reduces the occurrence of bacterial wilt disease;

[0029] The present invention makes full use of the organic fertilizer added with functional microorganisms, which not only increases the fertilizer efficiency and improves the fertilizer utilization rate, but also inhibits the reproduction and growth of pathogenic bacteria in the soil, reduces and prevents the occurrence of diseases, and at the same time can induce the plant's own disease resistance mechanism in the plant body through the rhizosphere ducts, thereby enhancing the disease resistance of crops;

[0030] The present invention further selects microbial inoculant and chemical agents to cooperate and assist in preventing and controlling bacterial wilt disease. By applying microbial fertilizer at an early stage, a rhizosphere biological barrier for the healthy growth of tobacco plants is formed, the defense ability against bacterial wilt germs is enhanced, and the disease incidence is reduced, so the efficiency increase is more significant. BRIEF DESCRIPTION OF THE DRAWINGS

[0031] Figure 1 It is a process diagram of the prevention and control method of the present invention. Detailed implementation manners

[0032] The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. All other embodiments obtained by those of ordinary skill in the art based on the embodiments of the present invention without creative efforts shall fall within the protection scope of the present invention.

[0033] Please refer to Figure 1 , the present invention provides a technical solution: The tobacco used in the following experimental examples is the flue-cured tobacco variety Yunyan 87. The Bacillus amyloliquefaciens and Lysobacter were provided by Yunnan Agricultural University. The organic fertilizer, the microbial inoculant agricultural phage + Bacillus subtilis (Yipin Nongbo), 42% trichloroisocyanuric acid, zhongshengmycin, and thiabendazole were provided by the Jinggu County Tobacco Company in Puer City.

[0034] Unless otherwise specified, the experimental methods and conditions used in the following experimental examples are all conventional experimental methods and conditions in the art, and relevant experimental manuals, well-known literatures or manufacturer's instructions can be referred to. Unless otherwise defined, the meanings of all technical and scientific terms used herein are the same as those commonly understood by those of ordinary skill in the art to which the present invention belongs.

[0035] Example 1

[0036] A green prevention and control method for tobacco bacterial wilt includes the following steps:

[0037] Step 1, rotate tobacco with corn.

[0038] Step 2, perform matrix soil bacterium mixing treatment or bacterized seedling treatment before tobacco seedling raising. Mix the matrix soil with the fermented bacterial liquid in a ratio of 20:1 and then sow and raise seedlings.

[0039] Step 3, select oyster shell powder products with a particle size of 100 meshes (0.15 mm), evenly spread them on the tobacco field before ploughing, and then plough to mix them evenly with the soil, or apply them in strips according to the situation. When the pH is between 4.5 and 5.0, the application rate is 100 kg / 666.7 m2; when the pH is between 5.0 and 5.5, the application rate is 65 kg / 666.7 m2; when the pH is between 5.5 and 5.9, the application rate is 30 kg / 666.7 m2. Or 20 d to 30 d before transplanting, deeply plough and apply 50 kg to 100 kg of quicklime per 666.7 m2.

[0040] Step 4: Apply organic fertilizer with disease-suppressing function and antagonistic microbial agents. The specific method is as follows: Before applying the organic fertilizer, mix the microbial agents (Bacillus amyloliquefaciens, Lysobacter) with the organic fertilizer at a ratio of 50 kg / t. If the amount is small and difficult to mix evenly, first mix 50 kg - 100 kg of organic fertilizer with 50 kg of the microbial agents, and then evenly mix it with 1 t of organic fertilizer, and apply it as a base fertilizer when making ridges in the tobacco field.

[0041] Step 5: The application rate of the organic fertilizer with disease-suppressing function and antagonistic microbial agents is 60 kg of biological bacterial fertilizer applied in holes during tobacco seedling transplantation, and the number of applications is 1 time.

[0042] Step 6: The microbial preparation is a 150 - 250-fold solution of Bacillus amyloliquefaciens and Lysobacter, and the time for root soaking treatment is 10 - 30 min.

[0043] Step 7: Conduct intertillage and soil heaping 20 d - 25 d after transplantation, and additionally apply a 150 - 300-fold solution of the microbial agent agricultural phage + Bacillus subtilis (Yipin Nongbo) and a 150 - 250-fold solution of Bacillus amyloliquefaciens and Lysobacter, with a dosage of 300 mL / plant - 500 mL / plant.

[0044] Step 8: Conduct secondary high soil heaping on the tobacco rhizosphere 35 d - 40 d after transplantation, and at the same time, irrigate the roots with an 800 - 1000-fold solution of 42% trichloroisocyanuric acid, with a dosage of 300 mL / plant - 500 mL / plant.

[0045] Step 9: 7 d - 10 d after the secondary high soil heaping, alternately irrigate the roots with Zhongshengmycin and an 800 - 1000-fold solution of thiabendazole, with a dosage of 300 mL / plant - 500 mL / plant.

[0046] Step 10: During the topping period to the harvesting period, promptly pull out and destroy the diseased plants at the disease center, and disinfect the soil around the diseased plants with 1% lime water to prevent the spread of the disease.

[0047] Example 2

[0048] A green prevention and control method for tobacco bacterial wilt, comprising the following steps:

[0049] Step 1: Rotate tobacco with corn.

[0050] Step 2: Conduct substrate soil mixing with bacteria treatment or bacteria-infected seedling treatment before tobacco seedling raising. Mix the substrate soil with the fermented bacterial liquid at a ratio of 20:1 and then sow and raise seedlings.

[0051] Step 3: Select oyster shell powder products with a particle size of 100 mesh (0.15 mm), evenly spread them on the tobacco field before plowing, and then plow to mix them evenly with the soil, or apply them in furrows according to the situation. When the pH is between 4.5 and 5.0, the application rate is 100 kg / 666.7 m2; when the pH is between 5.0 and 5.5, the application rate is 65 kg / 666.7 m2; when the pH is between 5.5 and 5.9, the application rate is 30 kg / 666.7 m2. Or 20 d to 30 d before transplanting, deeply plow and spread 50 kg to 100 kg of quicklime per 666.7 m2.

[0052] Step 4: Apply organic fertilizers with disease-suppressing functions and antagonistic microbial agents. The specific method is as follows: Before applying organic fertilizers, mix microbial agents (Bacillus amyloliquefaciens, Lysobacter) with organic fertilizers at a ratio of 50 kg / t. If it is difficult to mix evenly due to a small amount, first mix 50 kg to 100 kg of organic fertilizers with 50 kg of microbial agents and then evenly mix them with 1 t of organic fertilizers, and apply them as base fertilizers when ridging the tobacco field.

[0053] Step 5: The application rate of organic fertilizers with disease-suppressing functions and antagonistic microbial agents is 90 kg of biological bacterial fertilizer applied in holes when transplanting tobacco seedlings, and the number of application times is 1 time.

[0054] Step 6: The microbial preparation is a 150- to 250-fold solution of Bacillus amyloliquefaciens and Lysobacter, and the soaking root treatment time is 10 to 30 min.

[0055] Step 7: Conduct intertillage and soil mulching 20 d to 25 d after transplanting, and additionally apply 150- to 300-fold solutions of agricultural phage + Bacillus subtilis (Yipin Nongbo) and 150- to 250-fold solutions of Bacillus amyloliquefaciens and Lysobacter, with a dosage of 300 mL / plant to 500 mL / plant.

[0056] Step 8: 35 d to 40 d after transplanting, conduct secondary high soil mulching on the tobacco rhizosphere, and at the same time, irrigate the roots with an 800- to 1000-fold solution of 42% trichloroisocyanuric acid, with a dosage of 300 mL / plant to 500 mL / plant.

[0057] Step 9: 7 d to 10 d after secondary high soil mulching, alternately irrigate the roots with 800- to 1000-fold solutions of Zhongshengmycin and Tioxymetone, with a dosage of 300 mL / plant to 500 mL / plant.

[0058] Step 10: During the topping period to the harvesting period, promptly pull out and destroy the diseased plants at the disease center, and disinfect the soil around the diseased plants with 1% lime water to prevent the spread of the disease.

[0059] Example 3

[0060] A green prevention and control method for tobacco bacterial wilt, comprising the following steps:

[0061] Step 1: Rotate tobacco with corn.

[0062] Step 2: Before tobacco seedling raising, perform substrate soil mixing with bacteria treatment or bacteria - treated seedling treatment. Mix the substrate soil with the fermented bacterial liquid in a ratio of 20:1, and then sow and raise seedlings.

[0063] Step 3: Select oyster shell powder products with a particle size of 100 mesh (0.15 mm), evenly spread them on the tobacco field before ploughing, and then plough to mix them evenly with the soil, or apply them in furrows according to the situation. When the pH is between 4.5 and 5.0, the application rate is 100 kg / 666.7 m²; when the pH is between 5.0 and 5.5, the application rate is 65 kg / 666.7 m²; when the pH is between 5.5 and 5.9, the application rate is 30 kg / 666.7 m². Or 20 d - 30 d before transplanting, deeply plough and apply 50 kg - 100 kg of quicklime per 666.7 m².

[0064] Step 4: Apply organic fertilizers with disease - suppressing functions and antagonistic microbial agents. The specific method is as follows: before applying organic fertilizers, mix microbial agents (Bacillus amyloliquefaciens, Lysobacter) with organic fertilizers at a ratio of 50 kg / t. If the amount is small and difficult to mix evenly, first mix 50 kg - 100 kg of organic fertilizers with 50 kg of microbial agents, and then evenly mix them with 1 t of organic fertilizers, and apply them as base fertilizers when ridging the tobacco field.

[0065] Step 5: The application rate of organic fertilizers with disease - suppressing functions and antagonistic microbial agents is 120 kg of biological bacterial fertilizer applied in holes at the time of tobacco seedling transplanting, and the application frequency is 1 time.

[0066] Step 6: The microbial preparation is a 150 - 250 - fold liquid of Bacillus amyloliquefaciens and Lysobacter, and the soaking root treatment time is 10 - 30 min.

[0067] Step 7: Perform intertillage and soil banking 20 d - 25 d after transplanting, and additionally apply 150 - 300 - fold liquid of agricultural phage + Bacillus subtilis (Yipin Nongbo) and 150 - 250 - fold liquid of Bacillus amyloliquefaciens and Lysobacter, with a dosage of 300 mL / plant - 500 mL / plant.

[0068] Step 8: 35 d - 40 d after transplanting, perform secondary high soil banking on the tobacco rhizosphere, and at the same time, perform root irrigation treatment with 800 - 1000 - fold liquid of 42% trichloroisocyanuric acid, with a dosage of 300 mL / plant - 500 mL / plant.

[0069] Step 9: 7 d - 10 d after secondary high soil banking, alternately perform root irrigation treatment with Zhongshengmycin and 800 - 1000 - fold liquid of thiabendazole, with a dosage of 300 mL / plant - 500 mL / plant.

[0070] Step 10: Timely pull out and destroy the diseased plants at the center of the disease during the topping period to the harvesting period, and disinfect the soil around the diseased plants with 1% lime water to prevent the spread of the disease.

[0071] Control example

[0072] According to the method of Example 1, the difference is that no prevention and control agents are applied.

[0073] Test example

[0074] The effects of the present invention will be further described below in combination with the application of the present method in tobacco planting. The specific test process and results are as follows.

[0075] Apply the methods in the above-mentioned examples and control examples to the entire process of tobacco seedling raising period, transplanting period, and field period. On the 50th day after transplanting, select 10 tobacco plants with uniform growth vigor to measure their plant height, stem girth, maximum leaf length, maximum leaf width, and number of effective leaves, investigate the disease occurrence situation, and calculate the control effect.

[0076] Investigation and grading standards for tobacco bacterial wilt:

[0077] Grade 0: The whole plant is disease-free;

[0078] Grade 1: There are occasional chlorotic spots on the stem, or the leaves below 1 / 2 of the diseased side wither;

[0079] Grade 3: There are black stripes on the stem, but not exceeding 1 / 2 of the stem height, or the leaves from 1 / 2 to 2 / 3 of the diseased side wither;

[0080] Grade 5: The black stripes on the stem exceed 1 / 2 of the stem height but do not reach the top of the stem, or the leaves above 2 / 3 of the diseased side wither;

[0081] Grade 7: The black stripes on the stem reach the top of the stem, or all the leaves of the diseased plant wither;

[0082] Grade 9: The diseased plant is basically dead.

[0083] The calculation is carried out using the following formula:

[0084] Incidence rate (%) = (number of diseased plants / number of investigated plants) × 100

[0085] Disease index = × 100

[0086] Relative control (%) = × 100

[0087] The investigation and statistical results of the agronomic traits of flue-cured tobacco under different treatments are shown in Table 1. The results show that 50 days after transplanting, the plant height, maximum leaf length, and maximum leaf width of Example 1, Example 2, and Example 3 are significantly higher than those of the control example. In summary, using the prevention and control method provided by the present invention to prevent and control tobacco bacterial wilt at various stages of tobacco can significantly improve the agronomic traits of tobacco plants, thereby improving the ability of tobacco plants to resist bacterial wilt and reducing the occurrence of bacterial wilt.

[0088] Table 1. Effects of different treatments on the agronomic traits of flue-cured tobacco

[0089]

[0090] The investigation and statistical results of the control effects of different treatments on tobacco bacterial wilt are shown in Table 2. The results show that 50 days after transplanting, the incidence rate and disease index of Example 1, Example 2, and Example 3 are significantly lower than those of the control example. Among them, Example 1 has the best control effect on tobacco bacterial wilt, and its relative control effect reaches 75.37%.

[0091] Table 2. Effects of different treatments on the control effect of tobacco bacterial wilt

[0092]

[0093] Although the embodiments of the present invention have been shown and described, it will be understood by those of ordinary skill in the art that various changes, modifications, substitutions, and variations can be made to these embodiments without departing from the principles and spirit of the present invention. The scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A green prevention and control method for tobacco bacterial wilt, characterized in that: Including: S1. Tobacco rotation: Rotate and plant two crops on the same piece of land; S2. Prevention and control in the seedling stage: Add microbial inoculants to the seedling substrate; S3. Prevention and control in the transplanting stage: Conserve the tobacco-growing soil, select disease-free and strong seedlings, and dip the roots with microbial preparations. At the same time, apply organic fertilizers with disease-suppressing functions and antagonistic microbial inoculants by hole application; S4. Prevention and control in the field management stage: Respectively, conduct intertillage and soil mulching 20d - 25d after transplanting and apply additional microbial inoculants; 35d - 40d after transplanting, conduct secondary high soil mulching on the tobacco root and stem parts, and at the same time irrigate the roots with 42% trichloroisocyanuric acid solution; 7d - 10d after the secondary high soil mulching, alternately use Zhongshengmycin and Tilmicosin agents; timely pull out and destroy diseased plants from the topping stage to the harvesting stage, and disinfect with lime water to avoid the spread of the disease.

2. The green prevention and control method for tobacco bacterial wilt according to claim 1, characterized in that: The two crops in the tobacco rotation in S1 are flue-cured tobacco - maize.

3. The green prevention and control method of tobacco bacterial wilt according to claim 1, characterized in that: In S2, conduct substrate soil mixing with bacteria treatment or bacteria-treated seedling treatment before tobacco seeding. Mix the substrate soil and the fermented bacterial liquid at a ratio of 20:1 and then sow and raise seedlings.

4. A green prevention and control method for tobacco bacterial wilt according to claim 1, characterized in that: The conservation of the tobacco-growing soil is to select oyster shell powder products with a particle size of 100 mesh (0.15mm), evenly spread them on the tobacco field before plowing, and then plow to mix them evenly with the soil, or apply them in furrows according to the situation; The pH is between 4.5 and 5.0, and the application rate is 100 kg / 666.7 m2; The pH is between 5.0 and 5.5, and the application rate is 65 kg / 666.7 m2; The pH is between 5.5 and 5.9, and the application rate is 30 kg / 666.7 m2; Or 20d - 30d before transplanting, conduct deep plowing, and spread 50 kg - 100 kg of quicklime per 666.7 m2.

5. The green prevention and control method for tobacco bacterial wilt according to claim 1, characterized in that: The organic fertilizer with disease-suppressing functions and the antagonistic microbial inoculants are composed of the organic fertilizer used for tobacco and Bacillus amyloliquefaciens and Lysobacter enzymogenes. The specific method is: Before applying the organic fertilizer, mix the microbial inoculant and the organic fertilizer at a ratio of 50 kg / t. If it is difficult to mix evenly due to a small amount, first mix 50 kg - 100 kg of organic fertilizer with 50 kg of the microbial inoculant and then evenly mix it with 1 t of organic fertilizer, and apply it as a base fertilizer when making ridges in the tobacco field.

6. The green control method for tobacco bacterial wilt according to claim 1, characterized in that: The application rate of the organic fertilizer with disease-suppressing functions and the antagonistic microbial inoculants is to apply 60 - 120 kg of biological bacterial fertilizer by hole application when transplanting tobacco seedlings, and the number of applications is 1 time.

7. A green control method for tobacco bacterial wilt according to claim 1, characterized in that: The microbial preparation is a 150 - 250-fold solution of Bacillus amyloliquefaciens and Lysobacter enzymogenes, and the time for root dipping treatment is 10 - 30 min.

8. The green prevention and control method for tobacco bacterial wilt according to claim 1, characterized in that: In S4: Conduct intertillage and soil mulching 20d - 25d after transplanting and apply additional microbial inoculants, namely, agricultural phage + Bacillus subtilis at 150 - 300 times and Bacillus amyloliquefaciens and Lysobacter enzymogenes at 150 - 250 times, with a dosage of 300 mL / plant - 500 mL / plant.

9. The green control method for tobacco bacterial wilt according to claim 1, characterized in that: In S4, 35d - 40d after transplanting, conduct secondary high soil mulching on the tobacco root and stem parts, and at the same time conduct root irrigation treatment with an 800 - 1000-fold solution of 42% trichloroisocyanuric acid, with a dosage of 300 mL / plant - 500 mL / plant.

10. A green control method for tobacco bacterial wilt according to claim 1, characterized in that: In S4, after 7 to 10 days of secondary high earthing up, alternately use Zhongshengmycin and 800 - 1000 times liquid of thiabendazole for root irrigation, with the dosage of 300 mL / plant - 500 mL / plant. During the topping stage to the harvesting stage, promptly pull out and destroy the diseased plants at the disease center, and disinfect the soil around the diseased plants with 1% lime water to avoid the spread of the disease.

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