Preparation method of artificial seeds of desmodium triflorum

By preparing Sandou Jincao artificial seeds, using non-tissue culture methods and the technology of coating endosperm with functional substances, the problems of high production cost, low efficiency and long cycle of Sandou Jincao are solved, and low-cost and efficient seed preparation and application promotion are achieved.

CN120240323APending Publication Date: 2025-07-04SANMING AGRI SCI RES INST OF FUJIAN PROVINCE
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Patent Information

Application Number
CN202510615328.2
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2025-05-14
Publication Date
2025-07-04

AI Technical Summary

Technical Problem

Sandian Jincao has high production costs, low efficiency, long cycle, and difficult seed harvesting, which limits its large-scale application and promotion.

Method used

Artificial seeds were prepared by using the old mature stolons of three-point golden grass, and the germ was prepared by non-tissue culture method, and the endosperm was coated with functional substances. The secondary embedding technology of non-woven fabrics and water retention agents was combined to form artificial seeds.

Benefits of technology

The preparation process is simplified, costs are reduced, efficiency is improved, production cycle is shortened, and the application promotion of Sandian Jincao is promoted.

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Abstract

The invention discloses a method for preparing artificial seeds of desmodium triflorum, which comprises the following steps of: cutting an old and mature stolon into a seed section with a single bud, culturing, rooting and promoting bud to obtain an artificial germ, preparing a functional substance into artificial endosperm, and wrapping the artificial endosperm outside the artificial germ to obtain an artificial seed soft capsule. And finally, carrying out secondary embedding on the artificial seed soft capsule by using non-woven fabric and a water-retaining agent to form a desmodium triflorum artificial seed finished product. The artificial seeds are prepared in a non-tissue culture mode by simulating the structure of the seeds, the preparation method is simple, the defects of desmodium triflorum planting in the prior art are overcome, and application and popularization of desmodium triflorum can be accelerated.
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Description

Technical Field

[0001] The present invention relates to the technical field of plant artificial seed preparation, and more specifically to a method for preparing artificial seeds of Desmodium triflorum Background Art

[0002] As an important leguminous plant, Desmodium triflorum has broad application prospects in the fields of ecological restoration, medicinal development, and feed production. However, at present, Desmodium triflorum faces many limitations in production applications

[0003] Under the existing production mode, Desmodium triflorum is mainly produced using grass planting trays. Although this method can achieve a certain scale of seedling cultivation, it requires a large amount of land resources for cultivation and transplantation, and the costs of making, maintaining the grass planting trays and the investment in supporting facilities are high, greatly increasing the production cost of Desmodium triflorum. At the same time, there are difficulties in harvesting the seeds of Desmodium triflorum itself. Due to its small and scattered seeds, a large amount of manpower is required for harvesting, and the labor cost during the harvesting process remains high. Moreover, as a leguminous plant, the growth of Desmodium triflorum seedlings is extremely slow, and it takes a long time from seed germination to the formation of strong seedlings that can be transplanted. This not only prolongs the production cycle but also further increases the seedling cultivation cost and management difficulty

[0004] In summary, the traditional production method of Desmodium triflorum has problems such as high cost, low efficiency, and long cycle, seriously restricting the large-scale application and promotion of Desmodium triflorum. Therefore, developing a method for preparing artificial seeds of Desmodium triflorum with high efficiency and low cost has important practical significance for breaking through the current production bottleneck and accelerating the application and promotion of Desmodium triflorum in various fields Summary of the Invention

[0005] In view of this, the present invention uses the mature stolon tissue of Desmodium triflorum to prepare artificial seeds through treatment and embedding

[0006] To achieve the above object, the present invention adopts the following technical scheme

[0007] A method for preparing artificial seeds of Desmodium triflorum, comprising the following steps

[0008] Step 1: Preparation of artificial germ

[0009] (1.1) Collect mature stolons of Desmodium triflorum from the mother plant garden and cut them into seed segments of 5 - 8 mm with single buds

[0010] (1.2) Immerse them in a rooting and bud-promoting solution containing carbendazim, 6 - BA, and NAA for 2 - 3 h

[0011] (1.3) Lift it out and spread it flat in an environment with a temperature of 25-30°C and natural light. Sprinkle the germination promoter evenly, spray for 30-60 seconds each time, with an interval of 15 minutes, and continuously spray for 5-7 days until the buds and roots of the seed segments germinate, then artificial embryos are obtained;

[0012] Step Two: Preparation of artificial endosperm

[0013] Using a water-soluble fertilizer solution with N:P:K = 20:20:20 as the solvent, add sodium alginate, NAA, 6-BA, carbendazim, and penicillin, and mix evenly to obtain artificial endosperm;

[0014] Step Three: Preparation of artificial seeds

[0015] (3.1) Put the artificial embryos prepared in Step One into a plastic grid tray, then inject the artificial endosperm, and immerse it in a 2% (mass concentration) CaCl2 solution. After about 5 minutes, it forms a hard shell to form a soft capsule, that is, the artificial seed soft capsule of Desmodium triflorum is obtained;

[0016] (3.2) Wrap the surface of the artificial seed soft capsule with paraffin and put it into a non-woven micro-grid bag. Fill the empty space with a water-retaining agent, and seal the bag mouth after filling to form the finished product of Desmodium triflorum artificial seeds.

[0017] Preferably, in step (1.2), the concentration of carbendazim in the rooting and bud-promoting solution is 2 g / L, the concentration of 6-BA is 0.1-1.0 mg / L, and the concentration of NAA is 0.1-0.5 mg / L.

[0018] Preferably, in step (1.3), the germination promoter is a 1 g / L water-soluble fertilizer solution with N:P:K = 20:20:20, adding 2 g / L of carbendazim, 0.1 mg / L of 6-BA, and 0.5 mg / L of NAA.

[0019] Preferably, in step two, the concentration of the water-soluble fertilizer is 1 g / L, the mass concentration of sodium alginate is 3-5%, the content of NAA is 0.1-0.5 mg / L, the content of 6-BA is 0.1-1.0 mg / L, the content of carbendazim is 2 g / L, and the content of penicillin is 0.02 mg / L.

[0020] Preferably, in step (3.2), the non-woven micro-grid bag is made of polylactic acid.

[0021] Preferably, in step (3.2), the water-retaining agent is potassium polyacrylate. After the water-retaining agent absorbs water and swells, it can break through the non-woven material, similar to the way a seed breaks through the seed coat after absorbing water.

[0022] Preferably, in step (3.2), the surface of the non-woven micro-grid bag is coated with glutinous rice starch slurry and dried. Adding an appropriate amount of glutinous rice starch to the non-woven fabric can overcome the drawback that the non-woven fabric is too soft to be shaped, and make it processed into a shape similar to that of a glutinous rice capsule shell, that is, a non-woven fabric shell. Overcoming the drawback that the glutinous rice capsule shell disintegrates directly when encountering water, by controlling the thickness of the non-woven fabric capsule shell, it can disintegrate slowly within 5-7 days according to the germination characteristics of plants.

[0023] The non-woven micro-grid bag described in step (3.2) includes a main body and micro-grid bags evenly distributed on the main body. The main body is evenly distributed with the micro-grid bags of 0.5-2.0 mL specifications, and the thickness of the micro-grid bag is 5 gsm-15 gsm.

[0024] The thickness of the non-woven fabric of 5 gsm-10 gsm-15 gsm is proportional to the volume of the polylactic acid non-woven micro-grid bag of 0.5 ml-1.0 ml-2.0 ml. The thicker it is, the larger the volume of the micro-grid bag to be loaded, ensuring that the water-retaining agent loaded can break through the non-woven fabric within 5-7 days during the seed germination period.

[0025] Preferably, tear lines are provided around the micro-grid bags on the main body.

[0026] It can be seen from the above technical solutions that, compared with the prior art, the present invention discloses a method for preparing artificial seeds of Desmodium triflorum, and has the following beneficial effects:

[0027] The germ is prepared by non-tissue culture, and then the functional substance is used as the endosperm to coat the germ, providing nutrients for the germination of the germ and promoting its development. And through the secondary embedding technology, the seeds are prevented from sticking. The present invention prepares artificial seeds by non-tissue culture by imitating the structure of seeds. The preparation method is simple, and it overcomes the drawbacks of the prior art in planting Desmodium triflorum, and can accelerate the application and popularization of Desmodium triflorum. BRIEF DESCRIPTION OF THE DRAWINGS

[0028] In order to more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the following will briefly introduce the drawings required to be used in the description of the embodiments or the prior art. Obviously, the drawings in the following description are only the embodiments of the present invention. For those of ordinary skill in the art, other drawings can be obtained according to the provided drawings without creative efforts.

[0029] Figure 1 It is a side view of the non-woven micro-grid bag;

[0030] Figure 2 It is a top view of the non-woven micro-grid bag;

[0031] Figure 3 It is a top view of the non-woven micro-grid bag;

[0032] In the figure, 1 - main body, 2 - microcell bag, 3 - tear line. Detailed implementation mode

[0033] The technical solutions of the present invention will be clearly and completely described below in conjunction with the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. All other embodiments obtained by those of ordinary skill in the art based on the embodiments of the present invention without creative work belong to the scope of protection of the present invention.

[0034] As shown in the attached Figures 1 - 3 As shown, the non-woven microcell bag includes a main body 1 and microcell bags 2 evenly distributed on the main body. The main body 1 is evenly distributed with the microcell bags 2 of 0.5 - 2.0 mL specifications. The thickness of the microcell bag 2 is 5 gsm - 15 gsm, and the thickness of the non-woven fabric is 5 gsm - 10 gsm - 15 gsm, which is proportional to the volume of the polylactic acid non-woven microcell bag of 0.5 ml - 1.0 ml - 2.0 ml. The thicker it is, the larger the volume of the microcell bag to be loaded, ensuring that the water-retaining agent loaded can break through the non-woven fabric within 5 - 7 days during the seed germination period.

[0035] In some other specific improvement schemes, a tear line 3 is provided around the microcell bag 2 on the main body 1.

[0036] Example 1

[0037] In early April, stolons of Desmodium triflorum for overwintering dormancy were collected from the female parent garden and cut into about 5 mm bud-bearing seed segments.

[0038] ① Soak them in a rooting and bud-promoting solution containing 2 g / L of carbendazim, 0.1 mg / L of 6-BA, and 0.5 mg / L of NAA for 2 - 3 h; pick them up and spread them flat on the dense net in the artificial growth promotion room. The artificial growth promotion room relies on natural scattered light, controls the room temperature at 25 - 30 °C. Each dense net is 2 m high, 50 cm wide, and 2 m long. There are about 4 layers in each frame, with a spacing of 50 cm between each layer. Spraying equipment is arranged on each layer, and a rooting and bud-promoting agent (a solution of 1 g / L of Flower More general-purpose balanced water-soluble fertilizer (N:P:K = 20:20:20) added with 2 g / L of carbendazim, 0.1 mg / L of 6-BA, and 0.5 mg / L of NAA) is evenly sprayed. Continuously spray for 5 - 7 days until the buds and roots of the seed segments germinate to obtain the "germs" for preparing artificial seeds of Desmodium triflorum.

[0039] ②Put a pre-treated bud-bearing seed section of Desmodium triflorum into a plastic tray containing multiple plastic microcells. Each plastic microcell has a specification of 5 mm in width, 10 mm in length, 5 mm in height, and a volume of 0.25 ml, which is a long strip-shaped plastic microcell. Use a pipette to suck the artificial endosperm mixture (Flower More general-purpose balanced water-soluble fertilizer (N:P:K = 20:20:20) 1 g / L solution added with sodium alginate 40 g / L, carbendazim 2 g / L, penicillin 0.02 mg / L, 6-BA 0.1 mg / L, and NAA 0.5 mg / L) to fill the plastic microcells, and then soak them in a 2% (mass concentration) CaCl2 solution. After about 5 minutes, they form long strip-shaped soft capsules, obtaining Desmodium triflorum artificial seed soft capsules;

[0040] ③Secondary embedding of Desmodium triflorum artificial seed soft capsules. For secondary embedding, the polylactic acid non-woven fabric is pre-soaked and dried in a solution containing glutinous rice starch, sodium benzoate, or other preservative and insecticidal pesticides and pigments to make seed coatings of different colors. First, wrap the long strip-shaped soft capsules with a layer of paraffin (the purpose is to isolate the water-retaining agent and the soft capsule through paraffin to avoid the water analysis of the soft capsule), and then put them into the polylactic acid non-woven fabric microcell bags. The volume of each microcell bag is 0.5 mL, and the remaining space is filled with the agricultural water-retaining agent potassium polyacrylate. After filling, seal the bag mouth to obtain the finished Desmodium triflorum artificial seeds;

[0041] The specific structure of the polylactic acid non-woven fabric microcell bag is as Figures 1 - 2 shown. It can be that there are multiple microcell bags evenly distributed at a certain distance on a non-woven fabric with a specification of 42.5 cm * 42.5 cm, and the volume of each microcell bag is 0.5 - 2.0 mL; in this way, when planting, it can be spread out on the ground as a whole and then covered with a thin layer of fine soil, which is convenient for large-scale planting, saving labor and improving efficiency. It can also be that microcell bags with a volume of 0.5 - 2.0 mL are distributed on a non-woven fabric with a specification of 42.5 cm * 42.5 cm, as Figure 3 shown, with an easy-to-tear line 3 set around. When in use, it can be detached from the non-woven fabric and then sown.

[0042] Sowing of artificial seeds:

[0043] In the middle and late April, sow about 100 - 120 Desmodium triflorum artificial seeds per square meter of the finished Desmodium triflorum artificial seeds. Broadcast them on the plot that has been pre-loosened, harrowed, and leveled, and cover them with a 2 - 3 mm thin layer of soil and then cover with a light-transmitting green thin non-woven fabric (to prevent heavy rain erosion). Remove the covered non-woven fabric after the Desmodium triflorum artificial seeds germinate and take root. During this period, water management work should be done well. The germination rate of Desmodium triflorum artificial seeds reaches more than 80%, and the germination is relatively neat. After about 2 months, the effect of full-plot coverage can be achieved.

[0044] Example 2

[0045] In early April, collect the about 5 mm bud-bearing seed sections cut from the overwintering dormant stolons of Desmodium triflorum from the mother plant garden;

[0046] ① Pre-soak in a rooting and sprouting solution containing 2g / L of carbendazim, 0.1mg / L of 6-BA and 0.5mg / L of NAA for 2-3h; pick up and spread on the dense net of the artificial growth promotion room. The artificial growth promotion room relies on natural scattered light and controls the room temperature at 25-30°C. Each dense net is 2m high, 50cm wide and 2m long. Each rack has about 4 layers, and the spacing between each layer is 50cm. Spraying equipment is arranged on each layer, and the rooting and sprouting agent (1g / L solution of Huaduoduo universal balanced water-soluble fertilizer (N:P:K=20:20:20) is added with 2g / L of carbendazim, 0.1mg / L of 6-BA and 0.5mg / L of NAA) is evenly sprayed. Spray continuously for 5-7 days until the buds and roots of the seed stage germinate, and the "embryo" for preparing the artificial seeds of three-point golden grass is obtained;

[0047] ② Take two sections of the pretreated bud seed segments and put them into a plastic tray containing multiple plastic microgrids. The specifications of each plastic microgrid are 5 mm wide, 10 mm long, 5 mm high, and 0.25 ml long strip plastic microgrids. Use a straw to absorb artificial endosperm mixed solution (1 g / L solution of Huaduoduo universal balanced water-soluble fertilizer (N:P:K=20:20:20) with 40 g / L sodium alginate, 2 g / L carbendazim, 0.02 mg / L penicillin, 0.1 mg / L 6-BA, and 0.5 mg / L NAA) to fill the plastic microgrids, and then immerse them in a CaCl2 solution with a mass concentration of 2%. After about 5 minutes, long strip soft capsules are formed to obtain three-point golden grass artificial seed soft capsules;

[0048] ③ Secondary embedding of the three-point golden grass artificial seed soft capsule, the polylactic acid non-woven fabric for secondary embedding is pre-immersed in a solution containing glutinous rice starch, sodium benzoate or other preservatives and insecticides and pigments and dried to make seed coatings of different colors, the long strip soft capsule is first wrapped with a layer of paraffin and then put into a polylactic acid non-woven fabric micro-grid bag, the volume of each micro-grid bag is 0.5mL, and the remaining space is filled with agricultural water-retaining agent potassium polyacrylate. After loading, the bag mouth is sealed to obtain the three-point golden grass artificial seed finished product;

[0049] Among them, the specific structure of the polylactic acid non-woven micro-cell bag is as follows Figures 1 - 2 As shown, it can be a non-woven fabric with specifications of 42.5cm*42.5cm with multiple micro-grid bags evenly distributed at a certain distance, and the volume of each micro-grid bag is 0.5-2.0mL; in this way, when planting, the whole piece can be spread on the ground and covered with thin fine soil, which is convenient for large-scale planting, saving labor and improving efficiency. It can also be a non-woven fabric with specifications of 42.5cm*42.5cm with a distribution volume of 0.5-2.0mL, such as Figure 3 As shown, an easy-tear line 3 is arranged around the periphery, which can be buckled off from the non-woven fabric and then sowed when in use.

[0050] Sowing of artificial seeds:

[0051] In mid-to-late April, about 80-100 artificial seeds of Desmodium triflorum were sown per square meter. They were broadcast sown on a plot that had been pre-loosened, harrowed, and leveled, and covered with a 2-3 mm thin layer of soil, and then covered with a light-transmitting green non-woven fabric (to prevent heavy rain from washing away). The non-woven fabric was removed after the artificial seeds of Desmodium triflorum germinated and took root. During this period, water management work should be done well. The germination rate of the artificial seeds of Desmodium triflorum reached over 90%, and the germination was relatively neat. After about 2 months, the effect of full-coverage of the lawn could be achieved.

[0052] Example 3

[0053] In early September, mature stolons of Desmodium triflorum were collected from the mother plant garden and cut into seed segments with buds about 5 mm long.

[0054] ① First, soak them in a rooting and budding solution containing 2 g / L of carbendazim, 0.1 mg / L of 6-BA, and 0.5 mg / L of NAA for 2-3 hours; then pick them up and spread them flat on the dense net in the artificial growth chamber. The artificial growth chamber relies on natural scattered light, and the room temperature is controlled at 25-30 °C. Each dense net is 2 m high, 50 cm wide, and 2 m long, with about 4 layers, and the spacing between each layer is 50 cm. Spraying equipment is arranged on each layer, and a rooting and budding agent (a solution of 1 g / L of the general-purpose balanced water-soluble fertilizer of Flower More (N:P:K = 20:20:20) added with 2 g / L of carbendazim, 0.1 mg / L of 6-BA, and 0.5 mg / L of NAA) is evenly sprayed. After continuously spraying for 5-7 days until the buds and roots of the seed segments germinate, the "germs" for preparing artificial seeds of Desmodium triflorum are obtained.

[0055] ② Put one pretreated seed segment with a bud into a plastic tray containing multiple plastic microcells. Each plastic microcell has a specification of 5 mm wide, 10 mm long, 5 mm high, and a volume of 0.25 ml, which is a long-strip plastic microcell. Use a straw to suck the artificial endosperm mixture (a solution of 1 g / L of the general-purpose balanced water-soluble fertilizer of Flower More (N:P:K = 20:20:20) added with 50 g / L of sodium alginate, 2 g / L of carbendazim, 0.02 mg / L of penicillin, 0.1 mg / L of 6-BA, and 0.5 mg / L of NAA) to fill the plastic microcells, and then soak them in a 2% (mass concentration) CaCl2 solution. After about 5 minutes, they form long-strip soft capsules, and the soft capsules of artificial seeds of Desmodium triflorum are obtained.

[0056] ③ Secondary embedding of the soft capsules of artificial seeds of Desmodium triflorum. For secondary embedding, the polylactic acid non-woven fabric is pre-soaked in a solution containing glutinous rice starch, sodium benzoate, or other anti-corrosion and insecticidal pesticides and pigments, and then dried to make seed coatings of different colors. The long-strip soft capsules are first wrapped with a layer of paraffin and then put into a polylactic acid non-woven fabric microcell bag. The volume of each microcell bag is 0.5 mL, and the remaining space is filled with the agricultural water-retaining agent potassium polyacrylate. After filling, seal the bag mouth to obtain the finished products of artificial seeds of Desmodium triflorum.

[0057] Among them, the specific structure of the polylactic acid non-woven fabric microcell bag is asFigures 1 - 2 As shown, it can be a non-woven fabric with a specification of 42.5 cm * 42.5 cm, having a plurality of micro-grid bags evenly distributed at a certain distance, and the volume of each micro-grid bag is 0.5 - 2.0 mL. When planting, it can be laid flat on the ground and then covered with a thin layer of fine soil, which is convenient for large-scale planting, saves labor and improves efficiency. It can also be a non-woven fabric with a specification of 42.5 cm * 42.5 cm, on which micro-grid bags with a volume of 0.5 - 2.0 mL are distributed, such as Figure 3 As shown, an easy-to-tear line 3 is provided at the periphery. When in use, it can be detached from the non-woven fabric and then sown.

[0058] Sowing of artificial seeds:

[0059] In the middle and late September, about 100 - 120 artificial seeds of Desmodium triflorum per square meter are sown, scattered on a plot that has been pre-loosened, harrowed, leveled, and covered with a 2 - 3 mm thin layer of soil, and then covered with a light-transmitting green thin non-woven fabric (to prevent heavy rain erosion). The non-woven fabric covering is removed after the artificial seeds of Desmodium triflorum germinate and take root. During this period, water management work should be done well. The germination rate of the artificial seeds of Desmodium triflorum reaches over 80%, and the germination is relatively neat. After about 2 months, the effect of full-plot coverage can be achieved.

[0060] The various embodiments in this specification are described in a progressive manner. Each embodiment focuses on the differences from other embodiments. The same or similar parts among the various embodiments can be referred to each other. The above description of the disclosed embodiments enables those skilled in the art to implement or use the present invention. Various modifications to these embodiments will be obvious to those skilled in the art. The general principles defined herein can be implemented in other embodiments without departing from the spirit or scope of the present invention. Therefore, the present invention will not be limited to these embodiments shown herein, but rather to the widest scope consistent with the principles and novel features disclosed herein.

Claims

1. A preparation method of artificial seeds of Desmodium triflorum, characterized in that, It includes the following steps: Step 1: Preparation of artificial germ (1.1) Collect the mature stolons of Desmodium triflorum from the maternal garden and cut them into seed segments of 5 - 8 mm with a single bud; (1.2) Immerse them in a rooting and budding solution containing carbendazim, 6 - BA, and NAA for 2 - 3 h; (1.3) Lift them out and spread them flat in an environment with a temperature of 25 - 30 °C and natural light. Sprinkle the germination promoter evenly, spray for 30 - 60 s each time, with an interval of 15 min, and continuously spray for 5 - 7 days until the buds and roots of the seed segments germinate, then the artificial germ is obtained; Step 2: Preparation of artificial endosperm Using a water - soluble fertilizer solution with N:P:K = 20:20:20 as the solvent, add sodium alginate, NAA, 6 - BA, carbendazim, and penicillin, and mix evenly to obtain the artificial endosperm; Step 3: Preparation of artificial seeds (3.1) Put the artificial germ prepared in Step 1 into a plastic grid tray, then inject the artificial endosperm, and immerse it in a 2% (mass concentration) CaCl2 solution. After about 5 min, it forms a hard shell to form a soft capsule, that is, the artificial seed soft capsule of Desmodium triflorum is obtained; (3.2) Wrap the surface of the artificial seed soft capsule with paraffin and put it into a non - woven micro - grid bag. Fill the empty space with a water - retaining agent, seal the bag mouth after filling, and form the finished product of Desmodium triflorum artificial seeds.

2. The preparation method of the artificial seeds of Desmodium triflorum according to claim 1, characterized in that, In step (1.2), the concentration of carbendazim in the rooting and budding solution is 2 g / L, the concentration of 6 - BA is 0.1 - 1.0 mg / L, and the concentration of NAA is 0.1 - 0.5 mg / L.

3. The preparation method of the artificial seeds of Desmodium triflorum according to claim 1, characterized in that, In step (1.3), the germination promoter is a 1 g / L water - soluble fertilizer solution with N:P:K = 20:20:20, adding 2 g / L of carbendazim, 0.1 mg / L of 6 - BA, and 0.5 mg / L of NAA.

4. The preparation method of the artificial seeds of Desmodium triflorum according to claim 1, characterized in that, In step 2, the concentration of the water - soluble fertilizer is 1 g / L, the mass concentration of sodium alginate is 3 - 5%, the content of NAA is 0.1 - 0.5 mg / L, the content of 6 - BA is 0.1 - 1.0 mg / L, the content of carbendazim is 2 g / L, and the content of penicillin is 0.02 mg / L.

5. The preparation method of the artificial seeds of Desmodium triflorum according to claim 1, characterized in that, In step (3.2), the non - woven micro - grid bag is made of polylactic acid.

6. The preparation method of the artificial seeds of Desmodium triflorum according to claim 1, characterized in that, In step (3.2), the water - retaining agent is potassium polyacrylate.

7. The preparation method of the artificial seeds of Desmodium triflorum according to claim 1, characterized in that, In step (3.2), the surface of the non - woven micro - grid bag is coated with glutinous rice starch paste and dried.

8. The preparation method of the artificial seeds of Desmodium triflorum according to claim 1, characterized in that, In step (3.2), the non - woven micro - grid bag includes a main body and micro - grid bags evenly distributed on the main body. The main body is evenly distributed with micro - grid bags of 0.5 - 2.0 mL specification, and the thickness of the micro - grid bag is 5 gsm - 15 gsm.

9. The preparation method of the artificial seeds of Desmodium triflorum according to claim 8, characterized in that, An easy - tear line is provided at the four - week position of the micro - grid bag on the main body.