Antiviral composition containing dextran and alpha-bisabolol and application thereof

Abstract

The invention relates to an antiviral composition containing dextran and alpha-bisabolol, the weight ratio of dextran to alpha-bisabolol in the composition is (20: 1)-(1: 20), and the composition can be prepared into dosage forms such as soluble concentrate, suspending agent, emulsion in water and microemulsion. The composition is used for preventing and treating tobacco mosaic virus diseases, rice stripe diseases and vegetable and fruit virus diseases, is reasonable in components, has the effects of resisting viruses and improving the autoimmunity of crops, is good in antiviral effect, reduces the medication frequency and is low in medication cost, and the activity and the antiviral effect of the composition are not simple superposition of the activities of all the components but have a remarkable synergistic effect; and the composition has good safety to crops, and meets the safety requirements of pesticide preparations. The bactericidal composition has a good control effect on tobacco mosaic virus, rice stripe disease and virus diseases of vegetables, melons and fruits.

Description

Technical Field

[0001] The present invention relates to an antiviral composition containing glucomannan and α-bisabolol and its application, and particularly to an antiviral composition for preventing and controlling tobacco mosaic virus disease, rice stripe disease, and vegetable and fruit virus diseases. It is an antiviral composition composed of glucomannan as the first active ingredient and α-bisabolol as the second active ingredient, belonging to the technical field of pesticides. Background Art

[0002] Glucomannan, English name: Glucomannan, is composed of D-glucose and D-mannose monomers connected by β-1,4 glycosidic bonds. All glucomannans are acetylated, which makes them water-soluble.

[0003] Glucomannan can effectively inactivate viruses and has good control effects on diseases caused by various viruses. At the same time, it can promote photosynthesis, increase the accumulation of sugars and vitamins, improve the crop's own immunity and defense response, enhance the crop's stress resistance, and effectively promote crop growth, branching, flowering, fruiting, etc. Its elicitor for inducing systemic acquired resistance in crops exhibits unique biological activities. On the one hand, it can induce crops to produce excellent broad-spectrum disease resistance, not only inhibiting the early colonization, proliferation, and spread of virus diseases, but also inactivating the virus in vitro of the crop and inhibiting the long-distance spread of the virus. It has a high virus inhibition efficiency, good degradability, and the characteristics of no residue, safe operation, and strong stability. The control of plant diseases by glucomannan has been shown by the field efficacy tests of our company that glucomannan has good control effects on tobacco mosaic virus disease, rice stripe disease, and vegetable and fruit virus diseases. However, the glucomannan has a short effective period, resulting in more application times. Reasonable mixing of glucomannan can effectively exert its antiviral effect, delay the generation of resistance, and achieve better disease control effects. Currently, it mainly exists in the form of single agents. However, long-term continuous use of glucomannan has already produced certain resistance, and the single use cost is relatively high.

[0004] The common name of α-bisabolol is alpha-Bisabolol, and its chemical name is 1-methyl-4(1,5-dimethyl-1-hydroxyhexyl-4(5)-enyl) cyclohexene, CAS No.: 515-69-5. α-bisabolol is a non-toxic sesquiterpenol existing in natural essential oils. The domestic research on bisabolol mainly focuses on medicine and skin care. In the field of agricultural applications, research reports state that a sesquiterpenoid that can improve the disease resistance of rice was found in non-composite rice plants, and its regulatory mechanism was successfully analyzed. This research indicates that α-bisabolol may also be a kind of rice phytoalexin, and increasing its content in rice can improve the disease resistance of rice. However, there is no research report on the inhibitory effect of bisabolol on plant viruses.

[0005] Tobacco mosaic virus disease, vegetable and fruit virus diseases are common and frequently-occurring virus diseases, which often cause devastating disasters. At present, in the actual use process of curdlan in our company, it is found that curdlan has a good control effect on vegetable and fruit virus diseases, but the curdlan has a short effective period, resulting in more application times and high use costs. As a non-toxic sesquiterpenol existing in natural essential oils, α-bisabolol has not been promoted and used in the market, and there is also a problem of high single use cost. Summary of the Invention

[0006] The purpose of the present invention is to provide an antiviral composition with reasonable components, good antiviral effect, low drug cost and not easy to produce drug resistance.

[0007] Another purpose of the present invention is to provide the antiviral use of the above composition for preventing and treating tobacco mosaic virus disease, rice stripe disease, vegetable and fruit virus diseases.

[0008] In order to overcome the defects of existing single preparations, the technical solution of the present invention is solved as follows: The present invention contains curdlan as the first active ingredient and α-bisabolol as the second active ingredient, wherein the weight ratio of the first active ingredient to the second active ingredient is 20:1 to 1:20. The cumulative amount of the first active ingredient and the second active ingredient is 0.1% to 20% of the total weight of the composition, preferably 0.5% to 5%.

[0009] According to the methods well-known to those skilled in the art, the antiviral composition containing curdlan of the present invention can be formulated into any dosage form permitted in agriculture during actual application, and the preparation dosage form is a solution, suspension, emulsion in water, microemulsion.

[0010] For the solution dosage form, those skilled in the art are very familiar with using corresponding auxiliaries to complete the present invention. The auxiliaries that can be used are: emulsifiers such as agricultural emulsion 700# (common name: alkylphenol formaldehyde resin polyoxyethylene ether), agricultural emulsion 2201#, Span-60# (common name: sorbitan stearate), Tween-60# (common name: polyoxyethylene sorbitan stearate), TX-10 (common name: octylphenol polyoxyethylene (10) ether), agricultural emulsion 1601# (common name: triphenylethylphenol polyoxypropylene polyoxyethylene block polymer), agricultural emulsion 600#, agricultural emulsion 400# one or more of them; solvents such as methanol, ethanol, acetone, cyclohexanone, dimethylformamide, dimethyl sulfoxide, N-methylpyrrolidone, cyclohexanone, isophorone one or more of them.

[0011] For suspension concentrates, the auxiliary agents that can be used are: dispersants such as polycarboxylates, lignosulfonates, alkylnaphthalenesulfonates, TERSPERSE 2425 (produced by Huntsman Corporation in the United States, alkylnaphthalenesulfonate type), one or more of them; emulsifiers such as Nongru 700# (common name: alkylphenol formaldehyde resin polyoxyethylene ether), Nongru 2201, Span-60# (common name: sorbitan monostearate), Emulsifier T-60 (common name: polyoxyethylene sorbitan monostearate), Nongru 1601# (common name: phenethylphenol polyoxyethylene polyoxypropylene ether), TERSPERSE 4894 (produced by Huntsman Corporation in the United States), TERSPERSE 2500 (produced by Huntsman Corporation in the United States), one or more of them; wetting agents such as alkylphenol polyoxyethylene ether formaldehyde condensate sulfate, alkylphenol polyoxyethylene ether phosphate, phenethylphenol polyoxyethylene ether phosphate, alkyl sulfates, alkyl sulfonates, naphthalenesulfonates, TERSPERSE 2500 (produced by Huntsman Corporation in the United States), one or more of them; thickeners such as xanthan gum, polyvinyl alcohol, bentonite, one or more of them; preservatives such as formaldehyde, benzoic acid, sodium benzoate, one or more of them; defoamers such as silicone defoamers; antifreeze agents such as ethylene glycol, propylene glycol, glycerol, urea, inorganic salts such as sodium chloride, one or more of them.

[0012] For emulsion in water, the auxiliary agents that can be used are: emulsifiers such as nonylphenol polyoxyethylene (EO = 10) ether phosphate, triphenylethylphenol polyoxyethylene ether phosphate, Nongru 700# (common name: alkylphenol formaldehyde resin polyoxyethylene ether), Nongru 2201#, Span-60# (common name: sorbitan stearate), Tween-60# (common name: polyoxyethylene sorbitan stearate), TX-10 (common name: octylphenol polyoxyethylene (10) ether), Nongru 1601 (common name: triphenylethylphenol polyoxypropylene polyoxyethylene block polymer), Nongru 600#, Nongru 400#, one or more of them; solvents such as xylene, toluene, cyclohexanone, solvent naphtha (grades: S-150, S-180, S-200), one or more of them; antifreeze agents: ethylene glycol, propylene glycol, glycerol, urea, inorganic salts such as sodium chloride, one or more of them; thickeners such as xanthan gum, polyvinyl alcohol, bentonite, magnesium aluminum silicate, one or more of them; preservatives such as formaldehyde, benzoic acid, sodium benzoate, one or more of them, and the water is deionized water.

[0013] For microemulsions, the auxiliary agents that can be used include: emulsifiers such as calcium dodecylbenzenesulfonate (Agricultural Emulsifier 500#), Agricultural Emulsifier 700# (common name: alkylphenol formaldehyde resin polyoxyethylene ether), Agricultural Emulsifier 2201#, Span-60# (common name: sorbitan stearate), Tween-60# (common name: polyoxyethylene sorbitan stearate), TX-10 (common name: octylphenol polyoxyethylene (10) ether), Agricultural Emulsifier 1601 (common name: triphenylethylphenol polyoxypropylene polyoxyethylene block polymer), Agricultural Emulsifier 600#, Agricultural Emulsifier 400#, one or more of them; co-emulsifiers such as methanol, isopropanol, n-butanol, ethanol, one or more of them; solvents such as cyclohexanone, N-methylpyrrolidone, xylene, toluene, solvent oil (grades: S-150, S-180, S-200), one or more of them; and water is deionized water.

[0014] The components of the present invention are reasonable, with the functions of antiviral and improving the crop's own immunity. It has good antiviral effects, reduces the number of pesticide applications, and has low pesticide application costs. Moreover, its activity and antiviral effects are not a simple superposition of the activities of each component, but have a significant synergistic effect, slow down the generation of resistance, and are safe for crops, meeting the safety requirements of pesticide formulations. The present invention has good control effects on tobacco mosaic virus disease, rice stripe disease, and vegetable and fruit virus diseases. Detailed implementation mode

[0015] The following further illustrates the content of the present invention in conjunction with embodiments.

[0016] In order to control tobacco mosaic virus disease, rice stripe disease, and vegetable and fruit virus diseases in agricultural production, indoor bioassay synergistic research on the mutual compounding of glucan and α-bisabolol components was carried out.

[0017] The tests used tobacco mosaic virus, rice stripe virus, watermelon mosaic virus, and tomato yellow leaf curl virus (TYLCV) as test objects. The specific method was as follows: 1) The test agent α-bisabolol used the technical material produced by Wuhan PushiDa Biotechnology Co., Ltd., and the glucan technical material used the technical material produced by Shandong Kailinong Biotechnology Co., Ltd. The specific method is as follows: Prepare the original drug into the required test agents. First, set 5 different concentration gradients for the single agent and each mixed agent (set according to the geometric progression within the range of control efficacy from 5% to 90%). Select susceptible tobacco, rice, watermelon, and tomato varieties for potting. Wait until the seedlings grow to the 2- to 3-leaf stage for standby. The inoculation virus concentration is 8%, and the inoculation is carried out by sap rubbing. Each treatment has no less than 3 pots, with 5 plants in each pot. 24 hours after inoculation, evenly spray the agents on the standby tobacco, watermelon, zucchini, and tomato seedlings by the spraying method, and then cultivate them under suitable conditions. When the tested crops are fully diseased, investigate all the leaves of each pot of seedlings, and adopt the following grading method: Grade 0: Disease-free; Grade 1: The diseased area accounts for less than 5% of the whole leaf area; Grade 3: The diseased area accounts for 6% - 15% of the whole leaf area; Grade 5: The diseased area accounts for 16% - 25% of the whole leaf area; Grade 7: The diseased area accounts for 26% - 50% of the whole leaf area; Grade 9: The diseased area accounts for more than 50% of the whole leaf area.

[0018] Calculation method: Disease index = [∑(number of diseased leaves at each level × relative level value) / (total number of investigated leaves × 9)] × 100 Control efficacy (%) = [(control disease index - treatment disease index) / control disease index] × 100 Through the linear regression analysis between the probit value of the control efficacy and the logarithm of the series concentration, calculate the EC50 value of each agent, and calculate the co-toxicity coefficient (CTC) of the mixed agent by Sun Yunpei's method to evaluate the activity of the tested agents against the virus.

[0019] The co-toxicity coefficient (CTC) of the compound preparation ≥ 120 shows a synergistic effect; CTC ≤ 80 shows an antagonistic effect; 80 < CTC < 120 shows an additive effect.

[0019]

[0020]

[0021]

[0022]

[0023] As can be seen from Tables 1 to 4, when glucooligosaccharide is compounded with α-bisabolol in a weight ratio of 20:1 to 1:20, it has a good synergistic effect on tobacco mosaic virus disease, rice stripe disease, watermelon mosaic virus disease, and tomato yellow leaf curl virus disease.

[0024] Formulation Example 1 Weigh 0.5% glucooligosaccharide, 2.5% α-bisabolol, 4% Tween-60#, 2% Nongru 1601#, 10% dimethylformamide, and add ethanol to 100% by weight. After the above raw materials are mixed and stirred until completely dissolved, a 3% glucooligosaccharide·α-bisabolol soluble concentrate is prepared.

[0025] In the examples, the weight ratio of glucooligosaccharide to α-bisabolol can vary between 20:1 and 1:20, and the total weight component of the two is still 3%, forming new examples.

[0026] Formulation Example 2 Weigh 0.2% glucooligosaccharide, 2% α-bisabolol, 2% Nongru 1601#, 2% Nongru 600#, 8% N-methylpyrrolidone, and add isophorone to 100% by weight. After the above raw materials are mixed and stirred until completely dissolved, a 2.2% glucooligosaccharide·α-bisabolol soluble concentrate is prepared.

[0027] In the examples, the weight ratio of glucooligosaccharide to α-bisabolol can vary between 20:1 and 1:20, and the total weight component of the two is still 2.2%, forming new examples.

[0028] Formulation Example 3 Weigh 0.5% glucooligosaccharide, 1% α-bisabolol, 1% Nongru 1601#, 5% Nongru TX-10#, 10% cyclohexanone, and add isophorone to 100% by weight. After the above raw materials are mixed and stirred until completely dissolved, a 1.5% glucooligosaccharide·α-bisabolol soluble concentrate is prepared.

[0029] In the examples, the weight ratio of glucooligosaccharide to α-bisabolol can vary between 20:1 and 1:20, and the total weight component of the two is still 1.5%, forming new examples.

[0030] Formulation Example 4: Weigh 10% glucopolyose, 10% α-bisabolol, 3% TERSPERSE 4894 (produced by Huntsman Corporation, USA), 2% TERSPERSE 2500 (produced by Huntsman Corporation, USA), 3% TERSPERSE 2425 (produced by Huntsman Corporation, USA), 0.2% xanthan gum, 3% silica white, 5% ethylene glycol, 0.3% benzoic acid, 0.5% silicone defoamer (trade name: s-29 produced by Nanjing Sixin Applied Chemicals Co., Ltd.), and add deionized water to 100% by weight. The above raw materials are mixed, sheared and dispersed at high speed for 30 min, and then ground with a sand mill to obtain a 20% glucopolyose·α-bisabolol suspension.

[0031] In this example, the weight ratio of glucopolyose to α-bisabolol can vary between 20:1 and 1:20, and the total weight component of the two is still 20%, forming a new example.

[0032] Formulation Example 5: Weigh 10% glucopolyose, 0.5% α-bisabolol, 3% TERSPERSE 4894 (produced by Huntsman Corporation, USA), 1.5% TERSPERSE 2500 (produced by Huntsman Corporation, USA), 2% TERSPERSE 2425 (produced by Huntsman Corporation, USA), 0.2% xanthan gum, 3% silica white, 5% ethylene glycol, 0.3% benzoic acid, 0.5% silicone defoamer (trade name: s-29 produced by Nanjing Sixin Applied Chemicals Co., Ltd.), and add deionized water to 100% by weight. The above raw materials are mixed, sheared and dispersed at high speed for 30 min, and then ground with a sand mill to obtain a 10.5% glucopolyose·α-bisabolol suspension.

[0033] In this example, the weight ratio of glucopolyose to α-bisabolol can vary between 20:1 and 1:20, and the total weight component of the two is still 10.5%, forming a new example.

[0034] Formulation Example 6: Weigh 10% glucopolyose, 1% α-bisabolol, 4% TERSPERSE 4894 (produced by Huntsman Corporation, USA), 1.5% TERSPERSE 2500 (produced by Huntsman Corporation, USA), 1.5% TERSPERSE 2425 (produced by Huntsman Corporation, USA), 0.15% xanthan gum, 5% silica white, 5% ethylene glycol, 0.3% benzoic acid, 0.5% silicone defoamer (trade name: s-29 produced by Nanjing Sixin Applied Chemicals Co., Ltd.), and add deionized water to 100% by weight. The above raw materials are mixed, sheared and dispersed at high speed for 30 min, and then ground with a sand mill to obtain an 11% glucopolyose·α-bisabolol suspension.

[0035] In this example, the weight ratio of glucan oligosaccharide to α-bisabolol can vary between 20:1 and 1:20, and the total weight component of the two is still 11%, forming a new example.

[0036] Formulation Example 7: Weigh 15% glucan oligosaccharide, 3% α-bisabolol, 4% TERSPERSE 4894 (produced by Huntsman Corporation, USA), 1.5% TERSPERSE 2500 (produced by Huntsman Corporation, USA), 1% TERSPERSE 2425 (produced by Huntsman Corporation, USA), 0.18% xanthan gum, 2% silica white, 5% ethylene glycol, 0.3% benzoic acid, 0.5% silicone defoamer (trade name: s-29, produced by Nanjing Sixin Applied Chemicals Co., Ltd.), and add deionized water to 100% by weight. The above raw materials are mixed, sheared and dispersed at high speed for 30 min, and then milled with a sand mill to obtain an 18% glucan oligosaccharide·α-bisabolol suspension.

[0037] In this example, the weight ratio of glucan oligosaccharide to α-bisabolol can vary between 20:1 and 1:20, and the total weight component of the two is still 18%, forming a new example.

[0038] Formulation Example 8 Weigh 1% glucan oligosaccharide, 0.5% α-bisabolol, 3% agricultural emulsifier 2201#, 2% triphenylethylphenol polyoxyethylene ether phosphate, 2% Tween-60#, 2% Span-60#, 5% propylene glycol, 5% xylene, 5% cyclohexanone, 0.2% xanthan gum, 0.5% benzoic acid, and add deionized water to 100% by weight. The above raw materials are mixed and emulsified by high-speed shearing to obtain a 1.5% glucan oligosaccharide·α-bisabolol aqueous emulsion.

[0039] In this example, the weight ratio of glucan oligosaccharide to α-bisabolol can vary between 20:1 and 1:20, and the total weight component of the two is still 1.5%, forming a new example.

[0040] Formulation Example 9 Weigh 0.02% glucan oligosaccharide, 0.08% α-bisabolol, 2% agricultural emulsifier 2201#, 2% nonylphenol polyoxyethylene (EO = 10) ether phosphate, 2% triphenylethylphenol polyoxyethylene ether phosphate, 2% Span-60#, 5% ethylene glycol, 3% xylene, 3% cyclohexanone, 0.2% xanthan gum, 0.5% benzoic acid, and add deionized water to 100% by weight. The above raw materials are mixed and emulsified by high-speed shearing to obtain a 0.1% glucan oligosaccharide·α-bisabolol aqueous emulsion.

[0041] In this example, the weight ratio of glucan oligosaccharide to α-bisabolol can vary between 20:1 and 1:20, and the total weight component of the two is still 0.1%, forming a new example.

[0042] Formulation Example 10 Weigh 0.5% glucan, 1.5% α-bisabolol, 5% agricultural emulsifier 700#, 6% agricultural emulsifier 500#, 4% agricultural emulsifier 1601#, 10% cyclohexanone, 5% xylene, 5% n-butanol. After complete dissolution and thorough mixing, deionized water is added to 100% by weight. After stirring, a 2% glucan·α-bisabolol microemulsion is prepared.

[0043] In this example, the weight ratio of glucan to α-bisabolol can vary between 20:1 and 1:20, and the total weight component of the two is still 2%, forming a new example.

[0044] Formulation Example 11 Weigh 0.2% glucan, 0.3% α-bisabolol, 5% agricultural emulsifier 700#, 4% agricultural emulsifier 500#, 6% agricultural emulsifier 600#, 5% xylene, 10% isopropanol. After complete dissolution and thorough mixing, deionized water is added to 100% by weight. After stirring, a 5% glucan·α-bisabolol microemulsion is prepared.

[0045] In this example, the weight ratio of glucan to α-bisabolol can vary between 20:1 and 1:20, and the total weight component of the two is still 0.5%, forming a new example.

[0046] Formulation Example 12 Weigh 3% glucan, 2% α-bisabolol, 6% agricultural emulsifier 1601#, 3% agricultural emulsifier 500#, 7% agricultural emulsifier 600#, 15% cyclohexanone, 5% isopropanol. After complete dissolution and thorough mixing, deionized water is added to 100% by weight. After stirring, a 5% glucan·α-bisabolol microemulsion is prepared.

[0047] In this example, the weight ratio of glucan to α-bisabolol can vary between 20:1 and 1:20, and the total weight component of the two is still 5%, forming a new example.

[0048] Biological Example 1 Field Efficacy Test on Controlling Tobacco Virus Diseases For the investigation of controlling tobacco virus diseases, refer to "Guidelines for Field Efficacy Trials of Pesticides - Part 73: NY / T 464.73 - 2018 Fungicides for Controlling Tobacco Virus Diseases". The first application was carried out 1 day before tobacco transplanting, for preventive application on the seedbed. After transplanting and survival, application was continued twice continuously with an interval of 7 days. The control effect was investigated after application. The first investigation was carried out 10 days after the last application, and the last investigation was carried out before tobacco budding. In each plot, 5 representative points were randomly selected for sampling by the 5-point sampling method. 5 points were investigated in each plot, 10 plants were investigated at each point, and a total of 50 plants were investigated. The investigation was carried out by grading in units of plants. Grading method.

[0049] Disease severity classification method: Level 0: The whole plant is disease-free; Level 1: The heart veins are open and the leaves are slightly mosaic, but the whole plant has no obvious dwarfing; Level 3: 1 / 3 of the leaves are mosaic but not deformed, or the diseased plant is dwarfed to more than 3 / 4 of the normal plant height; Level 5: 1 / 3~1 / 2 of the leaves are mosaic, or a few leaves are deformed, or the main veins turn black, or the diseased plants are dwarfed to 2 / 3~3 / 4 of the normal plant height; Level 7: 1 / 2~2 / 3 of the leaves are mosaic, or deformed or the main and lateral veins are necrotic, or the diseased plants are dwarfed to 1 / 2~2 / 3 of the normal plant height; Level 9: The leaves of the entire plant are mosaic, severely deformed or necrotic, or the diseased plant is shrunk to more than 1 / 2 of the normal plant height.

[0050] Prevention and treatment effect (%) = (CK disease index - PT disease index) / CK disease index × 100

[0051] As can be seen from Table 5, after the mixture of glucan and α-bisabolol in a certain ratio, it has a good synergistic effect, and at a certain application dosage, it has a good control effect on tobacco virus diseases, and can significantly reduce the application amount of each active ingredient. All treatments in the experiment had no obvious adverse effects on the growth of tobacco, and the leaf color, yield, etc. were normal, with good safety.

[0052] Biological Example 2 Field Efficacy Test on Controlling Tomato Yellow Leaf Curl Virus Disease The control of tomato virus disease was carried out according to the "Guidelines for Field Efficacy Tests of Pesticides Part 8 NY / T 1464.8-2007 Fungicides for Control of Tobacco Virus Disease". The plots of test agent, control agent and blank control were arranged in random blocks with a plot area of ​​30m 2 , repeated 4 times, applied pesticides at the early stage of the disease, investigated the disease base before applying pesticides, applied pesticides three times, with an interval of 7 days each time, and investigated the control effect before the next application of pesticides and 7-14 days after application of pesticides. The investigation adopted the random sampling method of 5 points in each plot, 5 points in each plot, and 6 plants at each point, Grading method: Grade 0: no symptoms; Level 1: clear veins, light mosaic; Level 3: mosaic of heart leaves and middle leaves; Level 5: The heart leaves and middle leaves are mosaic, a few leaves are deformed, wrinkled or the plant is slightly dwarfed; Level 7: Severe mosaic, most leaves are deformed, shrunken or the plant is dwarfed; Level 9: Severe mosaic, obvious leaf deformity and linear leaves, severe dwarfing of the plant, and even death.

[0053] Calculation method of drug efficacy:

[0054]

[0055] In the formula: CK0 and CK1 are the disease index before and after spraying in the blank control area respectively; PT0 and PT1 are the disease index before and after spraying in the medicament treatment area respectively.

[0056]

[0057] It can be seen from Table 6 that after mixing laminarin and α-bisabolol in a certain proportion, it has a good synergistic effect. At a certain application dose, it has a good control effect on tomato yellow leaf curl virus disease and can significantly reduce the application amount of each active ingredient. All treatments in the experiment have no obvious adverse effects on the growth of tomatoes, and the leaf color, yield, etc. are normal, and the safety is good.

[0058] To sum up, the present invention for preventing and treating tobacco mosaic virus disease, rice stripe disease, and vegetable and fruit virus diseases has reasonable components, good antiviral effect, reduces the number of drug uses, has low drug cost, and has a significant synergistic effect, slows down the generation of resistance, has good safety for crops, and meets the safety requirements of pesticide formulations.

Claims

1. An antiviral composition containing glucan and α-bisabolol, characterized in that Comprising: A) The first active ingredient, glucooligosaccharide; B) The second active ingredient, α-bisabolol; The weight ratio of the first active ingredient to the second active ingredient is 20:1 to 1:

20.

2. The antiviral composition containing glucan and α-bisabolol according to claim 1, characterized in that: The cumulative amount of the first active ingredient and the second active ingredient is 0.1% to 20% of the total weight of the composition.

3. The antiviral composition containing glucan and α-bisabolol according to claim 2, characterized in that: The cumulative amount of the first active ingredient and the second active ingredient is 0.5% to 5% of the total weight of the composition.

4. The antiviral composition containing glucan and α-bisabolol according to claim 1, wherein: When the composition is actually used, it is formulated into any dosage form permitted in agriculture.

5. The antiviral composition containing glucan and α-bisabolol according to claim 4, wherein: The dosage form of the composition is a solution, a suspension, an emulsion in water, or a microemulsion.

6. Use of the antiviral composition containing glucooligosaccharide and α-bisabolol according to claim 1 in the prevention and treatment of tobacco mosaic virus disease, rice stripe disease, and viral diseases of vegetables and fruits.

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