Application of emamectin benzoate, attractant as well as preparation method and application of attractant
By using inducers prepared by A-dimension salt and combining raw materials such as grape juice, it effectively inhibits fruit fly reproduction, solves the environmental pollution and resistance problems caused by chemical pesticides, and achieves efficient and low-toxic fruit fly control effects.
Patent Information
- Application Number
- CN202510265654.5
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-03-07
- Publication Date
- 2025-07-04
AI Technical Summary
The existing fruit fly control methods mainly rely on chemical pesticides, which lead to environmental pollution and resistance problems, and cannot effectively inhibit fruit fly reproduction. The traditional methods have limited prevention and control effects.
Adipodyssium is used as the main component inducer, combined with grape juice, brown sugar, corn flour, yeast powder and agar powder, and atractylodes are prepared to inhibit fruit fly reproduction through low-concentration Adipodyssium mother liquor, and its reproductive toxicity is used to control fruit fly reproduction.
At extremely low doses, it significantly inhibits fruit fly reproduction, lasts for a long time, reduces population expansion, avoids environmental pollution, reduces resistance risks, and provides efficient and low-toxic prevention and control strategies.
Smart Images
Figure CN120240459A_ABST
Abstract
Description
Technical Field
[0001] The present invention relates to the technical field of pest control, and particularly to the application of emamectin benzoate and a lure and its preparation method and uses. Background Art
[0002] Fruit flies are one of the important pests in agricultural production. Especially in fruit and vegetable cultivation, Drosophila melanogaster causes serious damage to crops. The existing control methods mainly rely on chemical pesticides, but these pesticides are not only harmful to the environment but also may pose a threat to human health.
[0003] Fruit flies (Drosophila) are a type of insect with extremely strong reproductive ability. They have a short life cycle and rapid generation replacement, and the population can increase rapidly within a very short time. Female fruit flies can lay hundreds of eggs throughout their life cycle and can continue to reproduce under suitable conditions, resulting in an exponential growth of the fruit fly population. This high reproductive ability not only exacerbates the harm of agricultural pests but also brings serious problems in fields such as food processing, storage, and experimental research. For example, during the storage of fruits and vegetables, the over-reproduction of fruit flies will cause fruit spoilage, bacterial transmission, and even affect food safety; in the laboratory environment, the out-of-control number of fruit flies may interfere with the experimental process and reduce the accuracy of research. Therefore, effectively controlling the reproduction of fruit flies is one of the key strategies to reduce their harm.
[0004] Currently, the prevention and control of fruit flies mainly rely on chemical insecticides, biological control, and physical control. However, chemical insecticides often reduce the population by directly killing adults or larvae, but fruit flies are extremely easy to develop resistance, and long-term use may lead to a decline in the control effect. In addition, traditional insecticides usually cannot directly affect the reproductive ability of fruit flies, so that the surviving individuals can still quickly restore the population size. Although biological control and physical control can reduce the number of fruit flies to a certain extent, it is difficult to fundamentally inhibit their reproduction, and the control effect is often limited. Therefore, developing a new method that can directly inhibit the reproduction of fruit flies has important practical significance. Summary of the Invention
[0005] The purpose of the present invention is to overcome the deficiencies in the prior art and provide an application of emamectin benzoate and a lure and its preparation method and uses, using a lure prepared by adding emamectin benzoate to control the reproduction of fruit flies. A very low concentration of emamectin benzoate can achieve good results, which is efficient, low-toxic, and environmentally friendly.
[0006] To achieve the above purpose, the present invention is implemented by the following technical solutions:
[0007] In the first aspect, the present invention provides an application of emamectin benzoate for preparing a lure for inhibiting the reproduction of fruit flies.
[0008] Further, the fruit fly is Drosophila melanogaster or Drosophila suzukii.
[0009] In a second aspect, the present invention provides an attractant for inhibiting the reproduction of fruit flies. The raw materials for preparing the attractant include emamectin benzoate, and the emamectin benzoate is applied to the use provided by the present invention.
[0010] Furthermore, the attractant for inhibiting the reproduction of fruit flies is prepared from the following raw materials: emamectin benzoate mother liquor, grape juice, water, brown sugar, corn flour, yeast powder, agar powder, and propionic acid;
[0011] Among them, the volume ratio of the emamectin benzoate mother liquor, grape juice, and water is (0.005 - 0.01):(40 - 50):(50 - 60);
[0012] The mass ratio of the brown sugar, corn flour, yeast powder, and agar powder is (20 - 24):(10 - 12):(5 - 6):(1 - 2);
[0013] The concentration of the emamectin benzoate mother liquor is 5 - 10 mg / L;
[0014] The dosage of the agar powder is 5 - 10 g / L based on the volume of water;
[0015] The dosage of the propionic acid is 5 - 10 ml added to every 1 L of the attractant.
[0016] Furthermore, the attractant is prepared by a preparation method including the following steps: adding the emamectin benzoate mother liquor, grape juice, brown sugar, corn flour, yeast powder, and agar powder into water in proportion and mixing evenly, boiling and cooling, and then adding propionic acid in proportion and mixing evenly to obtain the attractant.
[0017] In a third aspect, the present invention provides a method for evaluating the reproductive toxicity of the attractant provided by the present invention using fruit flies, including: respectively placing the attractant and a control attractant in two traps, placing the two traps at two diagonals in a square insect net cage, placing a preset number of fruit flies in the central area of the insect net cage, taking single pairs of the surviving fruit flies in the two traps after culturing for 44 - 52 h, and counting the egg-laying amount of each fruit fly after culturing for 18 - 24 h;
[0018] Among them, the control attractant is prepared by replacing the emamectin benzoate mother liquor in the attractant with an equal amount of water.
[0019] Furthermore, for the preset number of fruit flies, the preset number is 250 - 300.
[0020] Furthermore, the fruit flies are Drosophila melanogaster or Drosophila suzukii.
[0021] In a third aspect, the present invention provides a use of the attractant for inhibiting the reproduction of fruit flies.
[0022] Fourthly, the present invention provides a use of the attractant in the control of fruit flies.
[0023] Compared with the prior art, the beneficial effects achieved by the present invention are as follows:
[0024] The present invention provides a use of emamectin benzoate for preparing an attractant for inhibiting the reproduction of fruit flies. By using the attractant prepared by adding emamectin benzoate to inhibit the reproduction of fruit flies, this inhibitory effect is not only persistent and stable, and the reproductive ability of fruit flies will not recover in a short time, but also significant effects can be exerted at a relatively low dose, which can effectively reduce the expansion of the fruit fly population. Compared with traditional insecticides, this agent has better selectivity, avoids environmental pollution, and at the same time reduces the risk of the development of fruit fly resistance.
[0025] The attractant for inhibiting the reproduction of fruit flies provided by the present invention can cause a serious decline in the fertility of fruit flies and then control the fruit fly population, has obvious development value, and the raw materials of the formula combination are easily available. The added content of emamectin benzoate is small, which avoids environmental pollution, is ecologically safe, has no residue, has an important application prospect in the prevention and control of fruit flies, and provides a brand-new strategy for the control of fruit flies. BRIEF DESCRIPTION OF THE DRAWINGS
[0026] Figure 1 It is a schematic diagram of the indoor device placement for the attractant trapping effect experiment provided by the embodiment of the present invention;
[0027] Figure 2 It is a bar chart of the attracting effect of the attractants provided by the embodiments and comparative examples of the present invention on Drosophila melanogaster;
[0028] Figure 3 It is a bar chart of the attractants provided by the embodiments and comparative examples of the present invention for inhibiting the egg-laying amount of Drosophila melanogaster;
[0029] Figure 4 It is a bar chart of the attractants provided by the embodiments and comparative examples of the present invention for inhibiting the egg-laying amount of Drosophila suzukii;
[0030] Figure 5 It is a bar chart of the attractants provided by the embodiments and comparative examples of the present invention for the persistence experiment of inhibiting the egg-laying amount of Drosophila melanogaster. DETAILED DESCRIPTION OF THE EMBODIMENTS
[0031] The present invention will be further described below with reference to the drawings. The following embodiments are only used to more clearly illustrate the technical solutions of the present invention and cannot be used to limit the protection scope of the present invention.
[0032] Example 1
[0033] This embodiment provides an attractant for inhibiting the reproduction of fruit flies. The attractant is prepared from the following raw materials: 0.00005 L of emamectin benzoate mother liquor, 0.4 L of grape juice, 0.5 L of water, 5 ml of propionic acid, 100 g of brown sugar, 50 g of corn flour, 25 g of yeast powder, and 5 g of agar powder.
[0034] The attractant is prepared by a preparation method including the following steps: Add emamectin benzoate mother liquor, grape juice, brown sugar, corn flour, yeast powder, and agar powder to water and mix evenly. After boiling and cooling, add propionic acid and mix evenly to obtain the attractant for inhibiting the reproduction of fruit flies.
[0035] Example 2
[0036] This embodiment provides an attractant for inhibiting the reproduction of fruit flies and its preparation method. Different from Example 1, the attractant is prepared from the following raw materials: 0.000075 L of emamectin benzoate mother liquor, 0.4 L of grape juice, 0.5 L of water, 5 ml of propionic acid, 100 g of brown sugar, 50 g of corn flour, 25 g of yeast powder, and 5 g of agar powder.
[0037] Example 3
[0038] This embodiment provides an attractant for inhibiting the reproduction of fruit flies and its preparation method. Different from Example 1, the attractant is prepared from the following raw materials: 0.0001 L of emamectin benzoate mother liquor, 0.4 L of grape juice, 0.5 L of water, 5 ml of propionic acid, 100 g of brown sugar, 50 g of corn flour, 25 g of yeast powder, and 5 g of agar powder.
[0039] Comparative Example 1
[0040] This comparative example provides an attractant and its preparation method. The attractant is prepared from the following raw materials: 0.00005 L of emamectin benzoate mother liquor, 0.4 L of grape juice, 0.5 L of water, 5 ml of propionic acid, 100 g of sucrose, and 25 g of agar powder.
[0041] The attractant is prepared by a preparation method including the following steps: After adding emamectin benzoate mother liquor, grape juice, sucrose, and agar powder to water and mixing, heat to boiling. After cooling, add propionic acid to obtain the attractant provided by this comparative example.
[0042] Comparative Example 2
[0043] This comparative example provides an attractant and its preparation method. Different from Example 1, the preparation method of the attractant includes:
[0044] Mix 0.4 L of grape juice, 0.5 L of water, 100 g of brown sugar, 50 g of corn flour, 5 g of yeast powder, and 5 agar powder, heat to boiling, and after cooling, add 5 ml of propionic acid to obtain the attractant provided by this comparative example.
[0045] A reproductive inhibition experiment was conducted on the attractants provided in Examples 1, 2, 3 and Comparative Example 2. The attractants provided in Examples 1, 2, 3 and Comparative Example 2 were added to Drosophila tubes, 4 ml for each Drosophila tube. After it solidified, 1 fully mated female Drosophila melanogaster was introduced into each Drosophila tube, with 50 biological replicates for each example or comparative example.
[0046] The experimental results are as Figure 3 shown. The results indicate that the addition of emamectin benzoate at different concentrations can significantly inhibit Drosophila egg-laying. The average number of eggs laid by a single normal female Drosophila per day is 15 - 25. After treatment, the average number of eggs laid by a single female per day is basically less than 5, and the inhibitory effect is extremely obvious, proving that the addition of extremely low concentrations of emamectin benzoate can well inhibit the reproduction of Drosophila melanogaster, with great development value.
[0047] An attractant effect experiment was conducted on the attractants provided in Example 1 and Comparative Example 1. As Figure 1 shown, the attractants provided in Example 1 and Comparative Example 1 were respectively placed in two traps. The traps were placed at two corners of an insect net cage of 30 cm × 30 cm × 30 cm. 300 Drosophila melanogaster were introduced into the insect net cage to ensure that the distance from the Drosophila to each trap was equal and they could freely choose. After culturing for 48 hours, the number of Drosophila melanogaster in the two traps was counted. The experiment was conducted 4 times, and the results are as Figure 2 shown. The number of Drosophila attracted by the attractant provided in Example 1 is much larger than that of the attractant provided in Comparative Example 1. The experimental results show that the attractant provided in Example 1 has an obvious attracting effect, is simple to prepare, and the raw materials are easily available, with obvious development value.
[0048] The attractants provided in Example 1 and Comparative Example 2 were respectively placed in two traps. The traps were placed at two corners of an insect net cage of 30 cm × 30 cm × 30 cm. 100 Drosophila suzukii were introduced into the insect net cage to ensure that the distance from the Drosophila to each trap was equal and they could freely choose. After culturing for 24 h, a spawning comparison experiment was conducted on the surviving pairs of Drosophila suzukii in the two traps. After 24 h, their spawning amounts were counted. Since the spawning amount of Drosophila suzukii itself is relatively low, the spawning amounts of Drosophila suzukii were counted in groups of five. 5 biological replicates were taken for each spawning comparison experiment.
[0049] The results are as Figure 4 shown. For Drosophila suzukii cultured in the trap with the attractant provided in Comparative Example 2, the spawning amount was 15 - 20 eggs. While for Drosophila suzukii cultured in the trap with the attractant provided in Example 1, the spawning amount of the attractant was extremely low and almost no eggs were laid, and the effect was extremely obvious, indicating that the attractant provided in Example 1 effectively inhibited the reproduction of Drosophila suzukii.
[0050] A reproductive inhibition persistence experiment was conducted on the attractants provided in Example 1 and Comparative Example 2. Different from the reproductive inhibition experiment, Drosophila melanogaster were taken out every day for 1 - 8 days after treatment for an oviposition comparison experiment, and their oviposition amounts were counted. The experimental results are as Figure 5 shown. As in the bar chart shown in Figure 5 , for the bar chart data of each day, the data on the left side of the bar chart each day is the result of the reproductive inhibition persistence experiment of the attractant provided in Comparative Example 2, and the data on the right side is the result of the reproductive inhibition persistence experiment of the attractant provided in Example 1. It can be seen from Figure 5 that after the attractant provided in Example 1, the oviposition amount of the female individuals of Drosophila melanogaster decreased significantly, almost reaching the effect of sterility, and this inhibitory effect could last continuously. Even after the 6th day, when the oviposition amount of some Drosophila began to recover, there were still differences compared with the control group, proving that the attractant provided by the present invention has a persistent and stable inhibitory effect on Drosophila reproduction.
[0051] The embodiments of the present invention have been described above in conjunction with the accompanying drawings. However, the present invention is not limited to the above - mentioned specific embodiments. The above - mentioned specific embodiments are merely illustrative rather than restrictive. Under the inspiration of the present invention, those of ordinary skill in the art can also make many forms without departing from the spirit and scope protected by the claims of the present invention. These all fall within the protection scope of the present invention.
Claims
1. Use of emamectin benzoate for preparing an attractant for inhibiting the reproduction of Drosophila melanogaster.
2. The use according to claim 1, wherein: The Drosophila melanogaster is Drosophila melanogaster or Drosophila suzukii.
3. An attractant for inhibiting the reproduction of Drosophila, characterized in that, The raw materials for preparing the attractant include emamectin benzoate, and the emamectin benzoate is applied to the use as described in claim 1.
4. The attractant according to claim 3, wherein The attractant for inhibiting the reproduction of Drosophila melanogaster is prepared from the following raw materials: emamectin benzoate mother liquor, grape juice, water, brown sugar, corn flour, yeast powder, agar powder and propionic acid; Among them, the volume ratio of the emamectin benzoate mother liquor, grape juice and water is (0.005 - 0.01):(40 - 50):(50 - 60); The mass ratio of the brown sugar, corn flour, yeast powder and agar powder is (20 - 24):(10 - 12):(5 - 6):(1 - 2); The concentration of the emamectin benzoate mother liquor is 5 - 10 mg / L; The dosage of the agar powder is 5 - 10 g / L based on the volume of water; The dosage of the propionic acid is 5 - 10 ml added to each 1 L of the attractant.
5. The attractant according to claim 4, characterized in that, The attractant is prepared by a preparation method including the following steps: adding the emamectin benzoate mother liquor, grape juice, brown sugar, corn flour, yeast powder and agar powder into water in proportion and mixing evenly, boiling and cooling, and then adding propionic acid in proportion and mixing evenly to obtain the attractant.
6. A method for evaluating the reproductive toxicity of the attractant according to any one of claims 3-5 using Drosophila melanogaster, characterized in that, Including: Placing the attractant and the control attractant in two traps respectively, placing the two traps at two diagonals in a square insect net cage, placing a preset number of Drosophila melanogaster in the central area of the insect net cage, taking single pairs of the surviving Drosophila melanogaster in the two traps after culturing for 44 - 52 h, and counting the egg laying amount of each Drosophila melanogaster after culturing for 18 - 24 h; Among them, the control attractant is prepared by replacing the emamectin benzoate mother liquor in the attractant with an equal amount of water.
7. The method according to claim 6, wherein: The preset number of Drosophila melanogaster, where the preset number is 250 - 300.
8. The method according to claim 6, characterized in that: The Drosophila melanogaster is Drosophila melanogaster or Drosophila suzukii.
9. Use of an attractant as described in any one of claims 3 - 5 for inhibiting the reproduction of Drosophila melanogaster.
10. Use of an attractant as described in any one of claims 3 - 5 in the control of Drosophila melanogaster.