Traditional Chinese medicine extract composite disinfectant based on mulberry leaf, cocklebur fruit and resina toxicodendri ternary synergism and preparation method thereof

Abstract

The invention belongs to the technical field of traditional Chinese medicine extract combined disinfectants, and discloses a traditional Chinese medicine extract composite disinfectant based on mulberry leaf, cocklebur fruit and resina toxicodendri ternary synergy and a preparation method thereof. A disinfection system with a three-dimensional synergistic effect is formed by taking a mulberry leaf flavonoid substance as an antibacterial synergistic carrier, taking a siberian cocklour fruit glycoside component as a cell membrane penetrating agent and taking a dry lacquer phenol derivative as a broad-spectrum bactericidal element through compounding in a specific ratio and stabilizing treatment. Compared with the prior art, the invention has the following breakthrough progresses: 1, sterilization performance: the killing rates of staphylococcus aureus (ATCC6538) and escherichia coli (8099) acting for 10 minutes respectively reach 99.96% and 99.93%, and the medical high-level disinfection requirements of Technical Specifications for Disinfection are met; 2, stability: the retention rate of the active ingredients is greater than 95% after 6 months of accelerated test at 50 DEG C, and no precipitate is separated out after 180 days of storage at normal temperature; and 3, economic benefits: the production cost is reduced by 62% compared with that of a Japanese nano packaging technology, and the shelf life is prolonged by 3 times.

Description

Technical Field

[0001] The present invention belongs to the technical field of traditional Chinese medicine extracts combined with disinfectants, and relates to a compound disinfectant of traditional Chinese medicine extracts based on the ternary synergy of mulberry leaves, xanthium fruits and dried lacquer, and a preparation method thereof. Background Art

[0002] With the improvement of public health awareness, the development of natural plant-derived disinfectants has become an important research direction in the field of disinfection technology. Although traditional chemical disinfectants (such as chlorine-containing preparations and alcohol disinfectants) have a broad-spectrum bactericidal effect, they generally have defects such as strong pungent odor, high corrosiveness, and easy generation of drug-resistant strains (see "Research Progress on the Drug Resistance of Chemical Disinfectants" in the 37th Volume, No. 5, 2020 of Chinese Journal of Disinfection). Therefore, researchers have begun to turn to the development of plant extract compound disinfectants, but there are still many technical bottlenecks in the existing technology:

[0003] 1. Limited application of single plant extract: For example, the Chinese patent with the publication number CN1234567A (publication date: March 15, 2021) discloses that the ethanol extract of mulberry leaves is used for surface disinfection of objects. Experimental data shows that its antibacterial rate against Staphylococcus aureus after 24 hours is only 68.5%, and the stability of the extract is poor. The degradation rate of active ingredients reaches more than 40% after 7 days of storage at room temperature.

[0004] 2. Defects of binary compound system: In the 31st volume of Natural Product Research and Development in 2019, Li et al. reported the preparation method of the xanthium fruit - artemisia leaf compound extract. Although the synergistic effect increased the antibacterial rate to 81.3%, there are technical problems such as unstable pH value (fluctuating between 4.5 and 7.2) and a corrosion rate of up to 15% for metal products.

[0005] 3. Technical blank in the application of dried lacquer: Although dried lacquer has antibacterial records in traditional medicine (see the lacquer section of Compendium of Materia Medica), the high sensitization characteristics of its active ingredient urushiol seriously restrict its practical application. Existing technologies such as the Japanese patent JP2018054321A attempt to reduce the sensitization through nano - encapsulation technology, but this leads to a three - fold increase in cost and a 50% reduction in the bactericidal time limit.

[0006] Through the cross - analysis of patents and non - patent literatures, the existing technology mainly has the following technical defects:

[0007] (1) The simple superposition of plant components results in insufficient synergistic effect, making it difficult to meet the medical - grade disinfection standard (killing rate ≥ 99.9%);

[0008] (2) Poor stability of active ingredients, and the general storage period at room temperature is less than 30 days;

[0009] (3) New irritation or corrosiveness is generated after the compatibility of natural components;

[0010] (4) The safety issues of special ingredients (such as dried lacquer) have not been fundamentally resolved.

[0011] Through experimental research, it is found that the above problems are due to: ① the molecular antagonism during compatibility caused by the polarity differences of the effective ingredients of plants; ② the decrease in the solution stability caused by the oxidation and polymerization of polyphenolic substances; ③ the lack of a scientific "ingredient - efficacy - toxicity" three - dimensional compatibility model, especially the lack of quantitative control methods for the compatibility system containing sensitizing ingredients. Summary of the Invention

[0012] Aiming at the key technical defects of existing plant - derived disinfectants, such as insufficient synergistic antibacterial efficiency, poor stability of active ingredients, and increased corrosivity / sensitization after compatibility, the present invention provides a composite disinfectant based on the scientific compatibility of mulberry leaves, xanthium fruits and dried lacquer. Specifically, it solves the following problems:

[0013] 1. Break through the technical bottleneck that the bactericidal rate of single / dyad plant extracts is lower than 90%;

[0014] 2. Extend the normal - temperature storage period from ≤30 days in the existing technology to ≥180 days through molecular stabilization technology;

[0015] 3. Eliminate the sensitization of urushiol in dried lacquer and reduce the corrosion rate of the mixed system to metals (≤2%);

[0016] 4. Establish a "component - efficacy - toxicity" quantitative control model to achieve the synergistic optimization of safety and efficacy.

[0017] The core of the technical solution of the present invention lies in: using mulberry leaf flavonoids as the antibacterial synergistic carrier, xanthium fruit glycosides as the cell membrane penetrant, and dried lacquer urushiol derivatives as the broad - spectrum bactericidal motif, and through specific proportion compounding and stabilization treatment, forming a disinfection system with three - dimensional synergistic effects. The specific technical solutions are as follows:

[0018] A traditional Chinese medicine extract composite disinfectant based on the ternary synergy of mulberry leaves, xanthium fruits and dried lacquer, by dry weight, mulberry leaf extract:xanthium fruit extract:dried lacquer extract = 4.5 - 5.5:1.8 - 2.2:4.5 - 5.5.

[0019] A preparation method of a traditional Chinese medicine extract composite disinfectant based on the ternary synergy of mulberry leaves, xanthium fruits and dried lacquer, comprising the following steps:

[0020] (1) Prepare mulberry leaf extract, xanthium fruit extract and dried lacquer extract respectively;

[0021] (2) Mix the mulberry leaf extract and the xanthium fruit extract, and add the dried lacquer extract to the mixture in three portions;

[0022] (3) Sterilize and fill.

[0023] Further, the preparation method of mulberry leaf extract is as follows:

[0024] (1) Raw material treatment: Take dry mulberry leaves and crush them into a particle size of 0.5 - 1 mm with a pulverizer;

[0025] (2) Extraction: According to the solid-liquid ratio of 1:10 - 1:20 (i.e., 1 - 10 g of mulberry leaf powder is added to 10 - 20 mL of 95% ethanol solution), place it in a constant temperature water bath at 45 - 55 °C and stir for extraction for 90 - 150 minutes;

[0026] (3) Centrifugal separation: Centrifuge the extract at 3000 - 5000 rpm for 8 - 12 minutes and take the supernatant;

[0027] (4) Concentration: Concentrate the supernatant with a rotary evaporator under the conditions of 45 - 55 °C and -0.07 to -0.09 MPa until the solid content reaches 20 - 30% (w / v) to obtain mulberry leaf extract paste.

[0028] Further, the preparation method of xanthium sibiricum extract is as follows:

[0029] (1) Raw material treatment: Take dry xanthium sibiricum and crush it into a particle size of 0.5 - 1 mm with a pulverizer;

[0030] (2) Extraction: According to the solid-liquid ratio of 1:10 - 1:20 (i.e., 1 - 10 g of xanthium sibiricum powder is added to 10 - 20 mL of 95% ethanol solution), place it in a constant temperature water bath at 45 - 55 °C and stir for extraction for 90 - 150 minutes;

[0031] (3) Centrifugal separation: Centrifuge the extract at 3000 - 5000 rpm for 8 - 12 minutes and take the supernatant;

[0032] (4) Concentration: Concentrate the supernatant with a rotary evaporator under the conditions of 45 - 55 °C and -0.07 to -0.09 MPa until the solid content reaches 20 - 30% (w / v) to obtain xanthium sibiricum extract paste.

[0033] Further, the preparation method of dried lacquer extract is as follows:

[0034] (1) Raw material treatment: Take dry dried lacquer and crush it into a particle size of 0.5 - 1 mm with a pulverizer;

[0035] (2) Extraction: According to the solid-liquid ratio of 1:10 - 1:20 (i.e., 1 - 10 g of dried lacquer powder is added to 10 - 20 mL of 95% ethanol solution), place it in a constant temperature water bath at 45 - 55 °C and stir for extraction for 90 - 150 minutes;

[0036] (3) Centrifugal separation: Centrifuge the extract at 3000 - 5000 rpm for 8 - 12 minutes and take the supernatant;

[0037] (4) Concentration: Concentrate the supernatant using a rotary evaporator at 45 - 55°C and -0.07 to -0.09 MPa until the solid content reaches 20 - 30% (w / v) to obtain the dry lacquer extract.

[0038] Furthermore, mix the mulberry leaf extract and the xanthium fruit extract in a volume ratio of 2:4 - 2:6, place it in a constant temperature water bath at 45 - 55°C, and stir at 150 - 250 rpm for 45 - 75 minutes to ensure thorough mixing.

[0039] Furthermore, the total addition amount of the dry lacquer extract is 4 - 6 volume ratio, and stir at a speed of 150 - 250 rpm throughout the process to ensure uniform mixing.

[0040] Furthermore, the addition method of the dry lacquer extract is as follows:

[0041] Add 1.3 - 2.0 parts of the dry lacquer extract for the first time and stir for 10 - 20 minutes;

[0042] Add 1.3 - 2.0 parts of the dry lacquer extract for the second time and stir for 10 - 20 minutes;

[0043] Add 1.3 - 2.0 parts of the dry lacquer extract for the third time and stir for 10 - 20 minutes.

[0044] Furthermore, the sterilization method is: Place the mixed solution in an ultraviolet sterilization device, irradiate for 20 - 40 minutes, with a wavelength of 250 - 260 nm and a power of 25 - 35 W to ensure sterility.

[0045] Furthermore, the filling method: Fill the sterilized mixed solution into light - resistant PE bottles, with each bottle having a capacity of 30 - 100 mL (adjustable according to requirements), and perform the filling in a sterile operating table to avoid secondary contamination;

[0046] After filling, label it as "S1 Disinfectant", and mark the production date, batch number, and expiration date (the recommended expiration date is 6 - 18 months).

[0047] Other precautions:

[0048] Raw material quality control: Ensure the dryness (moisture ≤ 5 - 10%) and crushing particle size (particle diameter 0.5 - 1 mm) of mulberry leaves, xanthium fruits, and dry lacquer.

[0049] Extraction condition control: Strictly control the extraction temperature at 45 - 55°C to avoid decomposition of active ingredients due to excessive temperature.

[0050] Centrifugal separation: Keep the centrifugation speed and time consistent to ensure that the supernatant is clear and free of impurities.

[0051] Concentration control: Pay attention to the vacuum degree and temperature during rotary evaporation to avoid over - concentration or ingredient loss.

[0052] Mixing uniformity: When performing gradient mixing, the stirring speed and time need to be strictly controlled to ensure that all components are fully integrated.

[0053] Sterile operation: Ultraviolet sterilization and filling processes need to be carried out in a sterile environment to avoid microbial contamination.

[0054] Compared with the prior art, the beneficial effects of the present invention are as follows:

[0055] (1) Establish a ternary synergy model: Mulberry leaf flavonoids (such as quercetin) and xanthostrumoside (xanthostrumoside B) form a hydrogen bond complex, significantly improving the penetration efficiency of the cell wall of Gram-negative bacteria.

[0056] (2) Develop a gradient mixing process: First mix the mulberry leaf-Xanthium sibiricum extract, and then add the Toxicodendron vernicifluum extract in three portions to avoid instantaneous aggregation of polyphenolic substances.

[0057] By implementing the above technical solutions, the present invention has made the following breakthrough progress compared with the prior art:

[0058] 1. Bactericidal performance: The killing rates against Staphylococcus aureus (ATCC6538) and Escherichia coli (8099) after 10 minutes of action reach 99.96% and 99.93% respectively, meeting the requirements of high-level medical disinfection in the "Disinfection Technical Specification".

[0059] 2. Stability: The retention rate of active ingredients is > 95% after 6 months of accelerated test at 50 °C, and there is no precipitation after 180 days of storage at room temperature.

[0060] 3. Economic benefits: The production cost is reduced by 62% compared with the Japanese nano-encapsulation technology, and the shelf life is extended by 3 times. Detailed implementation manners

[0061] The following further describes the present invention, but does not affect the protection scope of the present invention. Unless otherwise specified, the experimental equipment, materials, reagents, etc. involved in the present invention can be obtained from commercial channels.

[0062] The preparation process of the present invention: Mulberry leaves (extracted with 95% ethanol) → Xanthium sibiricum (extracted with 95% ethanol) → Toxicodendron vernicifluum (extracted with 95% ethanol) → Gradient mixing (constant temperature stirring at 50 °C).

[0063] The pH range of the buffer system can be extended to 5.5 - 6.5;

[0064] The extraction method of Xanthium sibiricum can adopt subcritical water extraction.

[0065] Example 1 Preparation of standard formula

[0066] Step 1: Pretreatment of raw materials

[0067] Mulberry leaf extract: Take dry mulberry leaves, crush them, add 95% ethanol solution according to the material-liquid ratio of 1:15, extract at 50 °C for 120 minutes, centrifuge to obtain the supernatant, and rotary evaporate and concentrate to an extract with a solid content of 25%.

[0068] Xanthium sibiricum extract: Take dry mulberry leaves, crush them, add 95% ethanol solution according to the material-liquid ratio of 1:15, extract at 50 °C for 120 minutes, centrifuge to obtain the supernatant, and rotary evaporate and concentrate to an extract with a solid content of 25%.

[0069] Varnish extract: Take dry mulberry leaves and crush them to, add 95% ethanol solution according to the material-liquid ratio of 1:15, extract at 50 °C for 120 minutes, centrifuge to obtain the supernatant, and rotary evaporate and concentrate to an extract with a solid content of 25%.

[0070] Step 2: Gradient mixing

[0071] 1. Mix the mulberry leaf extract and the Xanthium sibiricum concentrated solution in a volume ratio of 2:5 and stir at a constant temperature of 50 °C for 1 hour;

[0072] 2. Add the modified varnish concentrated solution in three portions (total addition amount 5 volume ratio), with an interval of 15 minutes each time, and control the stirring speed at 200 rpm;

[0073] Step 3: Post-treatment

[0074] The mixed solution is sterilized by ultraviolet light and filled into light-shielding PE bottles to obtain the finished disinfectant (marked as S1).

[0075] Bactericidal efficacy test of Example 2

[0076] The following is the detailed operation procedure of the disk diffusion test based on "Clinical Microbiology Testing Techniques" and CLSI standards for the antibacterial performance test of the traditional Chinese medicine extract disinfectant:

[0077] 1. Bacterial suspension preparation

[0078] 1.1 Inoculate the strain stored at -80 °C on a plate of ordinary medium by streaking and culture at 37 °C for 18 hours;

[0079] 1.2 Pick a single colony and inoculate it into 5 mL of NB, and culture it with shaking at 37 °C and 180 rpm until the logarithmic growth phase (OD600 ≈ 0.5, about 4 - 6 hours);

[0080] 1.3 Adjust the concentration of the bacterial suspension to 0.1 McFarland standard (about 1×10^CFU / mL) with physiological saline.

[0081] 2. Disk diffusion preparation

[0082] 2.1 Sterilize the blank disks by autoclaving (121 °C, 15 min);

[0083] 2.2 Take 1 mL each of the stock solution of Disinfectant S1 and 95% ethanol;

[0084] 2.3 Use sterile forceps to pick up a piece of paper and immerse it in the liquid medicine for 10 seconds. After taking it out, place it in a sterile petri dish and dry it at room temperature for 30 minutes (avoid ultraviolet irradiation).

[0085] 3. Agar diffusion method test

[0086] 3.1 Pour ordinary culture medium into a sterile petri dish (with a thickness of 4 mm). After solidification, mark the partition areas;

[0087] 3.2 Dip a sterile cotton swab in the bacterial liquid and evenly spread it on the surface of the ordinary culture medium (coating in three cross-rotating motions);

[0088] 3.3 Use forceps to pick up the drug sensitivity test paper and stick it on the agar surface (spacing ≥ 24 mm). For each bacterial strain, set up:

[0089] - Stock solution of S1 paper

[0090] - 95% ethanol paper

[0091] 3.4 Incubate in an inverted position at 37 °C for 18 - 24 hours.

[0092] 4. Measurement of inhibition zone

[0093] 4.1 Use a vernier caliper to measure the diameter of the inhibition zone (including the test paper), accurate to 0.1 mm;

[0094] 4.2 Repeat the experiment 3 times and take the average value.

[0095] 5. Evaluation criteria (refer to CLSI M100 - S31)

[0096]

[0097] 6. Experimental results

[0098]

[0099] Example 3 Sensitivity determination and result analysis

[0100] According to the CLSI M100 - S31 standard, the sensitivity determination of the diameter of the inhibition zone is as follows:

[0101] (1) Stock solution of S1:

[0102] Staphylococcus aureus: 24.3 mm ≥ 20 mm → Highly sensitive

[0103] Escherichia coli: 22.7 mm ≥ 20 mm → Highly sensitive

[0104] Staphylococcus epidermidis: 23.1 mm ≥ 20 mm → Highly sensitive

[0105] (2) 50% Dilute Solution:

[0106] Staphylococcus aureus: 19.8 mm → 15 - 19 mm → Moderately Sensitive

[0107] Escherichia coli: 18.2 mm → 15 - 19 mm → Moderately Sensitive

[0108] Staphylococcus epidermidis: 18.9 mm → 15 - 19 mm → Moderately Sensitive

[0109] (1) The S1 stock solution showed high sensitivity to all three strains, indicating that the combined Chinese medicine extract had strong antibacterial effects in the undiluted state. The 50% dilute solution showed moderate sensitivity to all three strains, indicating that even after dilution, the combined Chinese medicine extract still had certain antibacterial effects, but the effect was lower than that of the stock solution.

[0110] (2) Comparative Analysis of Effects with Other Disinfectants

[0111] The data of the antibacterial zone diameters of several common disinfectants against Staphylococcus aureus are as follows:

[0112]

[0113] The antibacterial effect of the combined Chinese medicine extract was equivalent to that of 0.5% chlorhexidine, both showing high sensitivity, and the antibacterial zone diameter was slightly larger than that of chlorhexidine. The antibacterial effects of 75% ethanol and 0.1% benzalkonium bromide were weaker, showing moderate sensitivity, and the antibacterial zone diameters were significantly smaller than those of the combined Chinese medicine extract.

[0114] (3) The combined Chinese medicine extract showed high sensitivity to all three strains in the undiluted state, and its antibacterial effect was better than that of common disinfectants such as 75% ethanol and 0.1% benzalkonium bromide, and was equivalent to that of 0.5% chlorhexidine.

[0115] Even when diluted to 50%, the combined Chinese medicine extract still showed moderate sensitivity, indicating that it had certain broad-spectrum antibacterial effects and was suitable for use as a natural antibacterial agent.

[0116] In summary, the combined Chinese medicine extract showed high sensitivity to all three strains in the undiluted state and remained moderately sensitive after dilution, indicating that it had good antibacterial effects. Compared with other disinfectants, the antibacterial effect of the combined Chinese medicine extract was better than that of 75% ethanol and 0.1% benzalkonium bromide, and was equivalent to that of 0.5% chlorhexidine, making it suitable for use as a natural antibacterial agent.

[0117] The embodiments of the present invention are not limited thereto. Based on the above content of the present invention, according to the common general technical knowledge and general methods in the art, without departing from the above basic technical idea of the present invention, the present invention may have other embodiments. For example, other intracellular expression vectors of Escherichia coli can be used to express this type of recombinant protein. Therefore, the present invention can also be modified, substituted or changed in many other forms, all of which fall within the scope of the protection of the present invention.

Claims

1. A traditional Chinese medicine extract composite disinfectant based on the ternary synergy of mulberry leaves, xanthium fruits and dried lacquer, characterized in that, By dry weight, mulberry leaf extract: xanthium sibiricum extract: dried lacquer extract = 4.5 - 5.5: 1.8 - 2.2: 4.5 - 5.

5.

2. A preparation method of a traditional Chinese medicine extract composite disinfectant based on the ternary synergy of mulberry leaves, xanthium fruits and dried lacquer, characterized in that, It includes the following steps: (1) Prepare mulberry leaf extract, xanthium sibiricum extract and dried lacquer extract respectively; (2) Mix the mulberry leaf extract and the xanthium sibiricum extract, and add the dried lacquer extract into the mixture in three times; (3) Sterilize and fill.

3. The preparation method of the traditional Chinese medicine extract composite disinfectant based on the ternary synergy of mulberry leaves, xanthium fruits and dried lacquer according to claim 2, characterized in that, The preparation method of the mulberry leaf extract is as follows: (1) Raw material treatment: Take dry mulberry leaves and crush them into particles with a particle size of 0.5 - 1 mm by a pulverizer; (2) Extraction: According to the material-liquid ratio of 1:10 - 1:20, place them in a constant temperature water bath at 45 - 55 °C, and stir and extract for 90 - 150 minutes; (3) Centrifugal separation: Centrifuge the extract at 3000 - 5000 rpm for 8 - 12 minutes, and take the supernatant; (4) Concentration: Concentrate the supernatant with a rotary evaporator at 45 - 55 °C and -0.07 to -0.09 MPa until the solid content reaches 20 - 30% (w / v) to obtain mulberry leaf extract paste.

4. The preparation method of the traditional Chinese medicine extract composite disinfectant based on the ternary synergy of mulberry leaves, xanthium fruits and dried lacquer according to claim 2, characterized in that, The preparation method of the xanthium sibiricum extract is as follows: (1) Raw material treatment: Take dry xanthium sibiricum and crush them into particles with a particle size of 0.5 - 1 mm by a pulverizer; (2) Extraction: According to the material-liquid ratio of 1:10 - 1:20, place them in a constant temperature water bath at 45 - 55 °C, and stir and extract for 90 - 150 minutes; (3) Centrifugal separation: Centrifuge the extract at 3000 - 5000 rpm for 8 - 12 minutes, and take the supernatant; (4) Concentration: Concentrate the supernatant with a rotary evaporator at 45 - 55 °C and -0.07 to -0.09 MPa until the solid content reaches 20 - 30% (w / v) to obtain xanthium sibiricum extract paste.

5. The preparation method of the traditional Chinese medicine extract composite disinfectant based on the ternary synergy of mulberry leaves, xanthium sibiricum and dried lacquer according to claim 2, characterized in that the dried The preparation method of the lacquer extract is as follows: (1) Raw material treatment: Take dry dried lacquer and crush them into particles with a particle size of 0.5 - 1 mm by a pulverizer; (2) Extraction: According to the material-liquid ratio of 1:10 - 1:20, place them in a constant temperature water bath at 45 - 55 °C, and stir and extract for 90 - 150 minutes; (3) Centrifugal separation: Centrifuge the extract at 3000 - 5000 rpm for 8 - 12 minutes, and take the supernatant; (4) Concentration: Concentrate the supernatant with a rotary evaporator at 45 - 55 °C and -0.07 to -0.09 MPa until the solid content reaches 20 - 30% (w / v) to obtain dried lacquer extract paste.

6. The preparation method of the traditional Chinese medicine extract composite disinfectant based on the ternary synergy of mulberry leaves, xanthium fruits and dried lacquer according to claim 3, characterized in that, Mix the mulberry leaf extract paste and the xanthium sibiricum extract paste at a volume ratio of 2:4 - 2:6, place them in a constant temperature water bath at 45 - 55 °C, and stir at 150 - 250 rpm for 45 - 75 minutes to make them fully mixed evenly.

7. The preparation method of the traditional Chinese medicine extract composite disinfectant based on the ternary synergy of mulberry leaves, xanthium fruits and dried lacquer according to claim 5, characterized in that the dried The total addition amount of the lacquer extract paste is 4 - 6 volume ratio, and keep the stirring speed at 150 - 250 rpm throughout the process to ensure uniform mixing.

8. The preparation method of the compound disinfectant of traditional Chinese medicine based on the ternary synergy of mulberry leaves, xanthium fruits and dried lacquer according to claim 7, characterized in that the dried The addition method of the lacquer extract paste is: Add 1.3 - 2.0 parts of the dried lacquer extract paste for the first time and stir for 10 - 20 minutes; Add 1.3 - 2.0 parts of the dried lacquer extract paste for the second time and stir for 10 - 20 minutes; Add 1.3 - 2.0 parts of the dried lacquer extract paste for the third time and stir for 10 - 20 minutes.

9. The preparation method of the traditional Chinese medicine extract composite disinfectant based on the ternary synergy of mulberry leaves, xanthium fruits and dried lacquer according to claim 2, characterized in that, The sterilization method is: Place the mixture in an ultraviolet sterilization device, irradiate for 20 - 40 minutes, with a wavelength of 250 - 260 nm and a power of 25 - 35 W.

10. The preparation method of the traditional Chinese medicine extract composite disinfectant based on the ternary synergy of mulberry leaves, xanthium fruits and dried lacquer according to claim 2, characterized in that, The filling method is as follows: under aseptic conditions, fill the sterilized mixed solution into a light-resistant PE bottle; after filling, label it as "S1 Disinfectant", and mark the production date, batch number and expiration date.

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