Method for inhibiting germination of stachys sieboldii tubers and prolonging shelf life of stachys sieboldii tubers

Abstract

The invention provides a method for inhibiting germination of stachys sieboldii tubers and prolonging the shelf life of the stachys sieboldii tubers, which comprises the following steps: S1, washing harvested fresh tubers with tap water, and cleaning soil on the surfaces; s2, drying the surface moisture at room temperature; s3, soaking the stachys sieboldii tubers in a hydrogen peroxide solution for 30 minutes; s4, airing the surfaces of the stachys sieboldii tubers at room temperature; and S5, bagging the stachys sieboldii, and storing in a dark place at room temperature. The method has the beneficial effects that the germination of the stachys sieboldii can be inhibited for more than one week under a room-temperature storage condition, the content of oligosaccharides such as stachyose, raffinose and verbascose is maintained not to be reduced, and the quality of the stachys sieboldii is maintained while the shelf life of the stachys sieboldii is prolonged when the stachys sieboldii is stored at room temperature; the method solves the problem that the quality is reduced due to the fact that the stachys sieboldii germinates after being harvested.

Description

Technical Field

[0001] The present invention relates to the field of postharvest biological regulation, and in particular, to a method for inhibiting the sprouting of Stachys sieboldii tubers and prolonging their shelf life. Background Art

[0002] Stachys sieboldii Miq is a plant of the genus Stachys in the Lamiaceae family and is mainly used as a special vegetable in China. It is native to China, and the earliest record can be traced back to "Supplementary Materia Medica" in 739 AD. Subsequently, it has been introduced to other countries in North America, Europe, and Asia. The tubers of Stachys sieboldii are rich in oligosaccharides, amino acids, flavonoids, proteins, and vitamins. These beneficial components endow Stachys sieboldii with functions of improving memory, reducing blood lipids, and treating nephritis. In recent years, the oligosaccharides in its tubers, especially stachyose, have attracted wide attention. Stachyose is a tetrasaccharide composed of 2 molecules of galactose, 1 molecule of glucose, and 1 molecule of fructose. Its metabolism requires digestive enzymes that can catalyze α-1,6-glycosidic bonds. The digestive systems of humans and monogastric animals lack this enzyme. Stachyose can enter the posterior end of the digestive tract directly without being absorbed by human intestinal cells, but can be fermented and utilized by intestinal Bifidobacterium and a few Lactobacilli. Its proliferation effect on the beneficial Bifidobacterium flora can reach a 40-fold growth rate, which is significantly better than that of isomaltooligosaccharide and fructooligosaccharide. Therefore, the intake of stachyose will not cause an increase in blood sugar and is especially suitable for diabetic patients. Stachyose mainly exists in plants of the Lamiaceae, Leguminosae, and Scrophulariaceae families, especially in Stachys sieboldii tubers with extremely high content, up to 20% at most, which is significantly higher than that of soybeans (the stachyose content is only 2% - 4%) and other plants.

[0003] Although Stachys sieboldii is rich in various nutrients and has a very high yield (1 - 2 tons per mu), its development and utilization are still lagging behind. Currently, Stachys sieboldii is mainly used to make pickles and salted vegetables. As a flavoring food, its value has not been deeply developed. The main limiting factor is that the tubers of Stachys sieboldii will germinate quickly at room temperature after harvest, resulting in a reduction in quality. Stachys sieboldii needs to be processed immediately after harvest, and its shelf life is extremely short. Therefore, inhibiting the germination of Stachys sieboldii under room temperature storage conditions will help maintain the quality of Stachys sieboldii and enhance its commercial value. However, there is currently no efficient control method for inhibiting the germination of Stachys sieboldii.

[0004] Stachys sieboldii is a herbaceous plant with strong growth and extremely strong stress resistance. In the early stage, the research group of the inventor found in the tissue culture research of Stachys sieboldii that it can achieve efficient tissue culture and rapid propagation without exogenous hormones. Analysis found that the endogenous cytokinin content of Stachys sieboldii was significantly higher than that of other plants (Improvement of Stachys sieboldii (Miq.) growth by virus elimination of shoot apices cultivated on media free of plant growth regulators. In Vitro Cellular & Developmental Biology - Plant. 2024, 60: 538–547.). Based on this, it is speculated that the easy germination characteristics of Stachys sieboldii tubers may be related to its extremely high endogenous cytokinin, and the method of inhibiting the germination of Stachys sieboldii tubers may also be different from that of other tuberous plants. Therefore, we made improvements and proposed a method for inhibiting the germination of Stachys sieboldii tubers and extending its shelf life. Summary of the Invention

[0005] The object of the present invention is: A method for inhibiting the germination of Stachys sieboldii under room temperature storage conditions, by inhibiting the germination of Stachys sieboldii tubers at room temperature, effectively delaying its shelf life, and laying a foundation for the diversified development and utilization of Stachys sieboldii. To achieve the above invention object, the present invention provides the following technical solutions: A method for inhibiting the germination of Stachys sieboldii tubers and extending its shelf life, comprising the following steps: S1. Rinse the freshly harvested tubers with tap water to wash away the soil on the surface;

[0006] S2. Place the Stachys sieboldii tubers at room temperature to dry the surface moisture;

[0007] S3. Immerse the Stachys sieboldii tubers in step S2 in a hydrogen peroxide solution with a concentration of 1.0 - 2.5 mg / L for 30 min;

[0008] S4. Dry the surface of the Stachys sieboldii tubers at room temperature;

[0009] S5. Store the Stachys sieboldii in bags at room temperature.

[0010] As a preferred technical solution of the present invention, S1. Rinse the freshly harvested tubers with tap water to wash away the soil on the surface specifically includes the following steps: S11. Prepare tools and materials:

[0011] Freshly harvested Stachys sieboldii tubers;

[0012] Tap water source, water temperature is 15 - 25 °C, water pressure is 0.2 - 0.4 MPa;

[0013] Wash the container, such as a large basin or sink, and the capacity should be selected according to the number of tubers to ensure that the tubers can be fully immersed in water;

[0014] S12. Operating steps:

[0015] Turn on the tap and adjust the water flow to an appropriate size, with a water flow rate of 8 - 12 L / min;

[0016] Put the Stachys affinis tubers into the cleaning container, and the volume ratio of tubers to water is 1:3 - 1:5;

[0017] Rinse the Stachys affinis tubers with tap water to ensure that the water flow can fully contact the surface of the tubers and wash away the soil; you can gently rub the tubers by hand to help remove the soil, but pay attention to avoid damaging the tubers; the rinsing time is 3 - 5 min;

[0018] Keep rinsing until the soil on the surface of the tubers is completely washed away.

[0019] As a preferred technical solution of the present invention, S2. Placing the Stachys affinis tubers at room temperature to dry the surface moisture specifically includes the following steps:

[0020] The Stachys affinis tubers after being washed in step S1;

[0021] An indoor space with good ventilation;

[0022] A clean tray or drying rack;

[0023] S22. Operating steps:

[0024] Place the clean tray or drying rack in an indoor space with good ventilation;

[0025] Evenly place the washed Stachys affinis tubers on the tray or drying rack, and there should be a certain distance between the tubers to ensure air circulation;

[0026] Control the room temperature at 20 - 25 °C and the relative humidity at 40% - 60%;

[0027] Let the Stachys affinis tubers dry naturally at room temperature for 2 - 3 h; during this process, the tubers should be turned regularly to ensure uniform evaporation of the surface moisture, and turn them every 30 min.

[0028] As a preferred technical solution of the present invention, S3. The specific steps of soaking the Stachys affinis tubers of S2 in a hydrogen peroxide solution with a concentration of 1.0 - 2.5 mg / L for 30 min:

[0029] S31. Prepare tools and materials:

[0030] The Stachys affinis tubers with the surface moisture dried in step S2;

[0031] A hydrogen peroxide solution with a concentration of 1.0 - 2.5 mg / L;

[0032] A container large enough to hold the Chinese artichoke tubers, such as a plastic basin or a glass tank;

[0033] S32. Operation steps:

[0034] Prepare the corresponding amount of hydrogen peroxide solution according to the number of Chinese artichoke tubers. For example, if there are 10 kg of Chinese artichoke tubers, according to the ratio of tuber to solution volume of 1:2, 20 L of hydrogen peroxide solution is required;

[0035] Pour the prepared hydrogen peroxide solution into the container;

[0036] Put the Chinese artichoke tubers with their surface moisture dried into the hydrogen peroxide solution, ensuring that the tubers are completely immersed in the solution;

[0037] The soaking time is 30 min. During this process, the solution can be appropriately stirred to ensure full contact between the tubers and the solution.

[0038] As a preferred technical solution of the present invention, step S4. Air-drying the surface of the Chinese artichoke tubers at room temperature includes the following steps:

[0039] S41. Prepare tools and materials: Chinese artichoke tubers soaked in hydrogen peroxide solution through step S3; a well-ventilated indoor space; a clean tray or drying rack;

[0040] S42. Operation steps: Place a clean tray or drying rack in a well-ventilated indoor space; evenly arrange the Chinese artichoke tubers soaked in hydrogen peroxide solution on the tray or drying rack, and there should be a certain distance between the tubers to ensure air circulation; control the room temperature at 20 - 25 °C and the relative humidity at 40% - 60%.

[0041] As a preferred technical solution of the present invention, S42. Let the Chinese artichoke tubers air-dry naturally at room temperature, and the air-drying time is 1 - 2 h; during this process, the tubers should be turned regularly to ensure uniform evaporation of the surface moisture, and turn them every 20 min.

[0042] As a preferred technical solution of the present invention, step S5. Storage of Chinese artichoke at room temperature after bagging specifically includes the following steps:

[0043] Step S51. Put the air-dried Chinese artichoke tubers into non-woven bags one by one, and the weight of the Chinese artichoke tubers in each bag is about 3.5 kg; after bagging is completed, use a rope to seal the bag, leaving an appropriate gap to ensure air circulation;

[0044] Step S52: Place the bag containing the Stachys affinis tubers in the storage room, with a spacing of 10 - 15 cm between the bags to avoid extrusion.

[0045] As a preferred technical solution of the present invention, the temperature of the storage room should be maintained at 12 - 14 °C, and the relative humidity should be controlled at 65% - 68%; a thermometer and a hygrometer can be used for monitoring, and if there is a deviation, the working status of the air conditioner and the humidifier should be adjusted in time;

[0046] During the storage process, check the status of the Stachys affinis tubers every 7 - 10 days to see if there are abnormal situations such as germination, rot, or discoloration; if problems are found, the problematic tubers should be taken out in time to avoid affecting the quality of other tubers.

[0047] As a preferred technical solution of the present invention, under room - temperature storage conditions, the germination of Stachys affinis tubers is inhibited for more than one week, and the contents of stachyose, raffinose, and verbascose oligosaccharides are maintained without decrease. While extending the shelf life under normal - temperature storage, the quality of Stachys affinis is maintained.

[0048] An application of a method for inhibiting the germination of Stachys affinis tubers and extending its shelf life in the agricultural product wholesale market of Stachys affinis, or the fresh food supermarket of Stachys affinis, or the e - commerce logistics distribution of Stachys affinis, characterized in that this method is used to treat the Stachys affinis tubers to extend its shelf life during the wholesale sales or logistics transportation process.

[0049] Compared with the prior art, the beneficial effects of the present invention: In the solution of the present invention, under room - temperature storage conditions, the germination of Stachys affinis tubers is inhibited for more than one week, and the contents of stachyose, raffinose, and verbascose oligosaccharides are maintained without decrease. While extending the shelf life under normal - temperature storage, the quality of Stachys affinis is maintained, solving the problem that the quality of Stachys affinis tubers decreases due to immediate germination after harvest, and laying a foundation for its diversified development. Description of the Drawings

[0050] Figure 1 It is the treatment effect of different inhibitors of the present invention on the germination of Stachys affinis tubers.

[0051] Figure 2 It is the experimental result of optimizing the hydrogen peroxide concentration of the present invention.

[0052] Figure 3 It is the sucrose content of the hydrogen peroxide treatment and the control group of the present invention stored for different times.

[0053] Figure 4 It is the glucose content of the hydrogen peroxide treatment and the control group of the present invention stored for different times.

[0054] Figure 5 It is the fructose content of the hydrogen peroxide treatment and the control group of the present invention stored for different times.

[0055] Figure 6 It is the raffinose content of the cottonseed treated with hydrogen peroxide in the present invention and the control group stored for different times.

[0056] Figure 7 It is the stachyose content of the cottonseed treated with hydrogen peroxide in the present invention and the control group stored for different times. Detailed implementation manners

[0057] To make the objectives, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Apparently, the described embodiments are some but not all of the embodiments of the present invention.

[0058] Therefore, the following detailed description of the embodiments of the present invention is not intended to limit the scope of the claimed present invention, but merely represents some embodiments of the present invention. All other embodiments obtained by those of ordinary skill in the art based on the embodiments of the present invention without creative efforts shall fall within the scope of protection of the present invention. It should be noted that, without conflict, the embodiments in the present invention and the features and technical solutions in the embodiments may be combined with each other. It should be noted that similar reference numerals and letters denote similar items in the following drawings. Therefore, once an item is defined in one drawing, it does not need to be further defined and explained in subsequent drawings.

[0059] Example 1: Please refer to Figures 1-7 , a method for inhibiting the sprouting of manna tubers and extending their shelf life, comprising the following steps: S1. Rinse the freshly harvested tubers with tap water to wash off the soil on the surface;

[0060] S2. Place the manna tubers at room temperature to dry the surface moisture;

[0061] S3. Immerse the manna tubers in step S2 in a hydrogen peroxide solution with a concentration of 1.0 - 2.5 mg / L for 30 min;

[0062] S4. Dry the surface of the manna tubers at room temperature;

[0063] S5. Store the manna in bags at room temperature.

[0064] S1. Rinsing the freshly harvested tubers with tap water to wash off the soil on the surface specifically includes the following steps: S11. Prepare tools and materials:

[0065] Freshly harvested manna tubers;

[0066] Tap water source with a water temperature of 15 - 25°C and a water pressure of 0.2 - 0.4 MPa;

[0067] Wash the container, such as a large basin or sink. The capacity should be selected according to the number of tubers to ensure that the tubers can be fully immersed in water;

[0068] S12. Operating steps:

[0069] Turn on the tap and adjust the water flow to an appropriate size, with a water flow rate of 8 - 12 L / min;

[0070] Put the Stachys affinis tubers into the cleaning container, and the volume ratio of tubers to water is 1:3 - 1:5;

[0071] Rinse the Stachys affinis tubers with tap water to ensure that the water flow can fully contact the surface of the tubers and wash away the soil; you can gently rub the tubers by hand to help remove the soil, but pay attention to avoid damaging the tubers; the rinsing time is 3 - 5 min;

[0072] Continue rinsing until the soil on the surface of the tubers is thoroughly washed away.

[0073] S2. Placing the Stachys affinis tubers at room temperature to dry the surface moisture specifically includes the following steps:

[0074] The Stachys affinis tubers after being washed in step S1;

[0075] An indoor space with good ventilation;

[0076] A clean tray or drying rack;

[0077] S22. Operating steps:

[0078] Place the clean tray or drying rack in an indoor space with good ventilation;

[0079] Evenly place the washed Stachys affinis tubers on the tray or drying rack, and there should be a certain distance between the tubers to ensure air circulation;

[0080] Control the room temperature at 20 - 25 °C and the relative humidity at 40% - 60%;

[0081] Let the Stachys affinis tubers dry naturally at room temperature for 2 - 3 h; during this process, the tubers should be turned regularly to ensure that the surface moisture is evenly dissipated, and turn them every 30 min.

[0082] S3. Specific steps for soaking the Stachys affinis tubers from S2 in a hydrogen peroxide solution with a concentration of 1.0 - 2.5 mg / L for 30 min:

[0083] S31. Prepare tools and materials:

[0084] The Stachys affinis tubers with the surface moisture dried in step S2;

[0085] Hydrogen peroxide solution with a concentration of 1.0 - 2.5 mg / L;

[0086] A container large enough to hold the Chinese artichoke tubers, such as a plastic basin or a glass tank;

[0087] S32. Operating steps:

[0088] Prepare the corresponding amount of hydrogen peroxide solution according to the number of Chinese artichoke tubers. For example, if there are 10 kg of Chinese artichoke tubers, according to the ratio of tuber to solution volume of 1:2, 20 L of hydrogen peroxide solution is required;

[0089] Pour the prepared hydrogen peroxide solution into the container;

[0090] Put the Chinese artichoke tubers with their surface moisture dried into the hydrogen peroxide solution, ensuring that the tubers are completely immersed in the solution;

[0091] The soaking time is 30 min. During this process, the solution can be appropriately stirred to ensure full contact between the tubers and the solution.

[0092] Step S4. Drying the surface of the Chinese artichoke tubers at room temperature includes the following steps:

[0093] S41. Prepare tools and materials: Chinese artichoke tubers soaked in hydrogen peroxide solution in step S3; a well-ventilated indoor space; a clean tray or drying rack;

[0094] S42. Operating steps: Place a clean tray or drying rack in a well-ventilated indoor space; evenly arrange the Chinese artichoke tubers soaked in hydrogen peroxide solution on the tray or drying rack, and there should be a certain distance between the tubers to ensure air circulation; control the room temperature at 20 - 25 °C and the relative humidity at 40% - 60%.

[0095] S42. Let the Chinese artichoke tubers dry naturally at room temperature for 1 - 2 h; during this process, the tubers should be turned regularly to ensure uniform evaporation of the surface moisture, and turn them every 20 min.

[0096] Step S5. Storing the Chinese artichoke at room temperature after bagging specifically includes the following steps:

[0097] Step S51. Put the dried Chinese artichoke tubers into non-woven bags one by one, and the weight of Chinese artichoke tubers in each bag is about 3.5 kg; after bagging, use a rope to seal the bag, leaving an appropriate gap to ensure air circulation;

[0098] Step S52. Place the bags containing Chinese artichoke tubers in the storage room, and keep a distance of 10 - 15 cm between the bags to avoid extrusion.

[0099] The temperature in the storage room should be maintained at 12 - 14°C, and the relative humidity should be controlled at 65% - 68%. A thermometer and a hygrometer can be used for monitoring. If there are any deviations, the working states of the air conditioner and the humidifier should be adjusted in a timely manner.

[0100] During the storage process, check the state of the Stachys affinis tubers every 7 - 10 days to see if there are any abnormal situations such as sprouting, rotting, or discoloration. If problems are found, the problematic tubers should be taken out in a timely manner to avoid affecting the quality of other tubers.

[0101] Under room - temperature storage conditions, the sprouting of Stachys affinis tubers can be inhibited for more than one week, and the contents of stachyose, raffinose, and verbascose oligosaccharides can be maintained without decrease. While extending the shelf life at room temperature, the quality of Stachys affinis is maintained.

[0102] An application of a method for inhibiting the sprouting of Stachys affinis tubers and extending their shelf life in the agricultural product wholesale market of Stachys affinis, or the fresh food supermarket of Stachys affinis, or the e - commerce logistics distribution of Stachys affinis, which is characterized in that this method is used to process the Stachys affinis tubers to extend their shelf life during the wholesale sales or logistics transportation process.

[0103] The principle of the method for inhibiting the sprouting of Stachys affinis tubers and extending their shelf life: Rinse the fresh tubers with tap water to wash off the soil on the surface. The main principle of this step is to remove the impurities and soil on the tuber surface through the flushing action of water flow, providing a clean basis for subsequent processing. Appropriate water temperature (15 - 25°C), water pressure (0.2 - 0.4 MPa), and water flow rate (8 - 12 L / min) can ensure effective removal of soil while avoiding damage to the tubers.

[0104] Place the tubers at room temperature to dry the surface moisture. In a well - ventilated indoor space, control the room temperature (20 - 25°C) and relative humidity (40% - 60%) to allow the moisture on the tuber surface to evaporate naturally. Regularly turning the tubers can promote the uniform dissipation of moisture and avoid local water accumulation leading to rotting. This step helps to reduce the moisture on the tuber surface and create suitable conditions for subsequent processing.

[0105] Soak the tubers in a hydrogen peroxide solution with a concentration of 1.0 - 2.5 mg·L-1 for 30 min. Hydrogen peroxide has certain bactericidal and sprout - inhibiting effects. Within this concentration range, hydrogen peroxide can effectively inhibit the sprouting of tubers while having less impact on the quality of tubers. Appropriate soaking time and the volume ratio of tubers to the solution can ensure that the tubers are fully in contact with the hydrogen peroxide solution and exert its sprout - inhibiting effect.

[0106] Air-dry the surface of the tubers at room temperature. Similar to step S2, by controlling the room temperature (20 - 25 °C) and relative humidity (40% - 60%), allow the moisture of the hydrogen peroxide solution remaining on the surface of the tubers to naturally evaporate, while preventing the tubers from absorbing excessive moisture. Regularly turning the tubers helps the moisture to disperse evenly and ensures the dryness of the tuber surface.

[0107] After bagging the air-dried tubers, store them at room temperature. Using non-woven bags can ensure a certain degree of air permeability and prevent the quality of the tubers from being affected due to lack of oxygen. Controlling the temperature (12 - 14 °C) and relative humidity (65% - 68%) of the storage room can create a relatively stable environment and delay the metabolic and sprouting processes of the tubers. Regularly checking the status of the tubers can help detect problems in a timely manner and take corresponding measures to prevent the problems from spreading and affecting the quality of other tubers.

[0108] Example 2: A method for inhibiting the sprouting of Stachys affinis tubers and extending their shelf life

[0109] S1. Rinse the freshly harvested tubers with tap water to wash away the soil on the surface

[0110] Prepare tools and materials: 5 kg of freshly harvested Stachys affinis tubers; a tap water source with a water temperature of 20 °C and a water pressure of 0.3 MPa; a large basin as the cleaning container with a capacity sufficient to accommodate the tubers to ensure that the tubers can be fully immersed in water;

[0111] Operation steps: Turn on the tap water faucet and adjust the water flow to an appropriate size, with a water flow rate of 10 L / min; place the Stachys affinis tubers in the cleaning container, with a volume ratio of tubers to water of 1:4; rinse the Stachys affinis tubers with tap water to ensure that the water flow can fully contact the surface of the tubers and wash away the soil; gently rub the tubers by hand to help remove the soil, but be careful not to damage the tubers; the rinsing time is 4 min; continue rinsing until the soil on the surface of the tubers is completely washed away.

[0112] S2. Place the Stachys affinis tubers at room temperature to air-dry the surface moisture. The Stachys affinis tubers after being washed in step S1;

[0113] An indoor space with good ventilation; clean trays;

[0114] Operation steps: Place the clean trays in an indoor space with good ventilation; evenly arrange the washed Stachys affinis tubers on the trays, and there should be a certain distance between the tubers to ensure air circulation;

[0115] Control the room temperature at 22 °C and the relative humidity at 50%; let the Stachys affinis tubers air-dry naturally at room temperature for 2.5 h; during this process, the tubers should be regularly turned to ensure the even dispersion of the surface moisture, and turn them every 30 min.

[0116] S3. Immerse the Stachys affinis tubercules obtained in S2 in a hydrogen peroxide solution with a concentration of 1.5 mg / L for 30 min. Prepare tools and materials: Stachys affinis tubercules with surface moisture dried after step S2; hydrogen peroxide solution with a concentration of 1.5 mg / L; a plastic basin large enough to hold the Stachys affinis tubercules.

[0117] Operation steps: Prepare an appropriate amount of hydrogen peroxide solution according to the number of Stachys affinis tubercules. For example, if there are 5 kg of Stachys affinis tubercules, according to the ratio of tubercules to solution volume of 1:2, 10 L of hydrogen peroxide solution is required. Pour the prepared hydrogen peroxide solution into the plastic basin. Put the Stachys affinis tubercules with surface moisture dried into the hydrogen peroxide solution to ensure that the tubercules are completely immersed in the solution. The soaking time is 30 min. During this process, the solution can be appropriately stirred to ensure full contact between the tubercules and the solution.

[0118] S4. Air-dry the surface of the Stachys affinis tubercules at room temperature. Prepare tools and materials: Stachys affinis tubercules soaked in hydrogen peroxide solution after step S3; a well-ventilated indoor space; a clean drying rack.

[0119] Operation steps: Place the clean drying rack in a well-ventilated indoor space. Arrange the Stachys affinis tubercules soaked in hydrogen peroxide solution evenly on the drying rack, and there should be a certain distance between the tubercules to ensure air circulation. Control the room temperature at 23 °C and the relative humidity at 50%.

[0120] Let the Stachys affinis tubercules air-dry naturally at room temperature for 1.5 h. During this process, the tubercules should be turned regularly to ensure uniform evaporation of surface moisture, and turn them once every 20 min.

[0121] S5. Store the Stachys affinis in bags at room temperature. Put the air-dried Stachys affinis tubercules into non-woven bags one by one, and the weight of Stachys affinis tubercules in each bag is about 3.5 kg. After bagging, use a rope to seal the bag, leaving an appropriate gap to ensure air circulation. Place the bags containing Stachys affinis tubercules in the storage room, and keep a 12-cm distance between the bags to avoid extrusion.

[0122] The temperature in the storage room should be maintained at 13 °C and the relative humidity should be controlled at 66%. A thermometer and a hygrometer can be used for monitoring, and if there is a deviation, the working status of the air conditioner and humidifier should be adjusted in time.

[0123] During the storage process, check the status of the Stachys affinis tubercules every 8 days to see if there are any abnormal situations such as sprouting, rotting or discoloration. If problems are found, the problematic tubercules should be taken out in time to avoid affecting the quality of other tubercules.

[0124] Example 3: A method for inhibiting the sprouting of Stachys affinis tubercules and extending their shelf life. S1. Rinse the freshly harvested tubercules with tap water to wash away the soil on the surface.

[0125] Prepare tools and materials: 8 kg of freshly harvested Stachys affinis tubers; a tap water source with a water temperature of 18°C and a water pressure of 0.25 MPa; a cleaning container, which is a sink, and its capacity should be selected according to the number of tubers to ensure that the tubers can be fully immersed in water;

[0126] Operation steps: Turn on the tap water faucet and adjust the water flow to an appropriate size, with a water flow rate of 9 L / min;

[0127] Put the Stachys affinis tubers into the cleaning container, with a volume ratio of tubers to water of 1:3.5; rinse the Stachys affinis tubers with tap water to ensure that the water flow can fully contact the surface of the tubers and wash away the soil; gently rub the tubers by hand to help remove the soil, but pay attention to avoid damaging the tubers; the rinsing time is 3.5 min; continue rinsing until the soil on the surface of the tubers is thoroughly washed away.

[0128] S2. Place the Stachys affinis tubers at room temperature to dry the surface moisture; the Stachys affinis tubers after being washed in step S1; a well-ventilated indoor space; a clean tray;

[0129] S22. Operation steps: Place the clean tray in a well-ventilated indoor space; evenly arrange the washed Stachys affinis tubers on the tray, and there should be a certain distance between the tubers to ensure air circulation; control the room temperature at 20°C and the relative humidity at 45%; let the Stachys affinis tubers dry naturally at room temperature for 2 h; during this process, the tubers should be turned regularly to ensure that the surface moisture is evenly dissipated, and turn them once every 30 min.

[0130] S3. Immerse the Stachys affinis tubers from S2 in a hydrogen peroxide solution with a concentration of 2 mg / L for 30 min

[0131] S31. Prepare tools and materials: The Stachys affinis tubers with the surface moisture dried in step S2; a hydrogen peroxide solution with a concentration of 2 mg / L; a glass tank large enough to hold the Stachys affinis tubers;

[0132] S32. Operation steps: Prepare the corresponding amount of hydrogen peroxide solution according to the number of Stachys affinis tubers; if there are 8 kg of Stachys affinis tubers, according to the volume ratio of tubers to solution of 1:2, 16 liters of hydrogen peroxide solution are needed; pour the prepared hydrogen peroxide solution into the glass tank; put the Stachys affinis tubers with the surface moisture dried into the hydrogen peroxide solution to ensure that the tubers are completely immersed in the solution; the immersion time is 30 min, and during this process, the solution can be appropriately stirred to make the tubers fully contact with the solution.

[0133] S4. Air-dry the surface of the Stachys sieboldii tuber at room temperature; S41. Prepare tools and materials: Stachys sieboldii tubers soaked in hydrogen peroxide solution through step S3; an indoor space with good ventilation; a clean drying rack; S42. Operating steps: Place the clean drying rack in an indoor space with good ventilation; evenly arrange the Stachys sieboldii tubers soaked in hydrogen peroxide solution on the drying rack, and there should be a certain distance between the tubers to ensure air circulation; control the room temperature at 22 °C and the relative humidity at 45%.

[0134] Let the Stachys sieboldii tubers air-dry naturally at room temperature for 1 h; during this process, the tubers should be turned regularly to ensure uniform evaporation of surface moisture, and turn them once every 20 min.

[0135] S5. Store the Stachys sieboldii at room temperature after bagging; S51. Put the air-dried Stachys sieboldii tubers into non-woven bags one by one, and the weight of the Stachys sieboldii tubers in each bag is about 3.5 kg; after bagging, use a rope to seal the bag, leaving an appropriate gap to ensure air circulation; S52. Place the bags containing Stachys sieboldii tubers in the storage room, and keep a 10-cm distance between the bags to avoid extrusion.

[0136] The temperature of the storage room should be maintained at 12 °C, and the relative humidity should be controlled at 65%; a thermometer and a hygrometer can be used for monitoring, and if there is a deviation, the working status of the air conditioner and humidifier should be adjusted in time;

[0137] During the storage process, check the status of the Stachys sieboldii tubers once every 7 days to see if there are abnormal situations such as sprouting, rotting or discoloration; if problems are found, the problematic tubers should be taken out in time to avoid affecting the quality of other tubers.

[0138] Experimental example: Effects of different treatment conditions on the germination rate of Stachys sieboldii tubers

[0139] This experiment aims to study the effects of different treatment conditions on the germination rate of Stachys sieboldii tubers. The experiment set up a control group and treatment groups with different concentrations. The specific treatment conditions and results are as follows:

[0140] 1. Experimental materials:

[0141] Fresh Stachys sieboldii tubers

[0142] 2. Experimental treatments:

[0143] Control group: Treated with tap water, concentration 0 mg / L.

[0144] ClO2 treatment group: Set three concentration gradients, which are 0.01 mg / L, 0.005 mg / L, and 0.001 mg / L respectively.

[0145] H2O2 treatment group: Three concentration gradients were set, namely 0.3 mg / L, 1.5 mg / L, and 3 mg / L.

[0146] 3. Experimental methods:

[0147] S1. Rinse the harvested fresh tubers with tap water to wash away the soil on the surface;

[0148] S2. Place the Stachys sieboldii tubers at room temperature to dry the surface moisture;

[0149] S3. Immerse the Stachys sieboldii tubers in step S2 in a hydrogen peroxide solution with a concentration of 1.0 - 3 mg / L for 30 min;

[0150] S4. Dry the surface of the Stachys sieboldii tubers at room temperature;

[0151] S5. Pack the Stachys sieboldii and store it at room temperature.

[0152] Experimental data:

[0153] Table 1 Germination rates of Stachys sieboldii tubers after 2 weeks of storage under different treatment conditions

[0154]

[0155] After 2 weeks of storage, the germination rate of Stachys sieboldii tubers treated with the control group (tap water) was 87.8%.

[0156] In the ClO2 treatment group, the germination rate of Stachys sieboldii tubers treated with a concentration of 0.01 mg / L was 100%, the germination rate of those treated with a concentration of 0.005 mg / L was 84%, and the germination rate of those treated with a concentration of 0.001 mg / L was 64%.

[0157] In the H2O2 treatment group, the germination rate of Stachys sieboldii tubers treated with a concentration of 0.3 mg / L was 34%, the germination rate of those treated with a concentration of 1.5 mg / L was 12.6%, and the germination rate of those treated with a concentration of 3 mg / L was 42%.

[0158] It can be seen from the experimental results that different treatment conditions have a significant impact on the germination rate of Stachys sieboldii tubers. In the ClO2 treatment group, the treatment effect of a concentration of 0.01 mg / L is relatively special, and the germination rate reaches 100%, while the treatment effects of other concentrations are different. In the H2O2 treatment group, the treatment with a concentration of 1.5 mg / L has a significant effect on inhibiting germination, and the germination rate is only 12.6%.

[0159] The above embodiments are only used to illustrate the present invention and do not limit the technical solutions described in the present invention. Although the present specification has described the present invention in detail with reference to the above respective embodiments, the present invention is not limited to the above specific implementation manners. Therefore, any modification or equivalent replacement made to the present invention; and all technical solutions and their improvements that do not depart from the spirit and scope of the invention are covered by the scope of the claims of the present invention.

Claims

1. A method for inhibiting the sprouting of Stachys affinis tubers and prolonging their shelf life, characterized in that, It includes the following steps: S1. Rinse the freshly harvested tubers with tap water to wash off the soil on the surface; S2. Place the Stachys affinis tubers at room temperature to air-dry the surface moisture; S3. Immerse the Stachys affinis tubers in step S2 in a hydrogen peroxide solution with a concentration of 1.0 - 2.5 mg / L for 30 min; S4. Air-dry the surface of the Stachys affinis tubers at room temperature; S5. Store the Stachys affinis in bags at room temperature.

2. A method for inhibiting the sprouting of Stachys affinis tubers and prolonging their shelf life according to claim 1, characterized in that, S1. Rinsing the freshly harvested tubers with tap water to wash off the soil on the surface specifically includes the following steps: S11. Prepare tools and materials: freshly harvested Stachys affinis tubers; Tap water source with a water temperature of 15 - 25 °C and a water pressure of 0.2 - 0.4 MPa; cleaning containers, including large basins or sinks, with a capacity selected according to the number of tubers to ensure that the tubers are immersed in water; S12. Operating steps: Turn on the tap water faucet and adjust the water flow to an appropriate size, with a water flow rate of 8 - 12 L / min; Put the Stachys affinis tubers into the cleaning container, with a volume ratio of tubers to water of 1:3 - 1:5; rinse the Stachys affinis tubers with tap water for 3 - 5 min.

3. A method for inhibiting the sprouting of Stachys affinis tubers and prolonging their shelf life according to claim 2, characterized in that, S2. Placing the Stachys affinis tubers at room temperature to air-dry the surface moisture specifically includes the following steps: The Stachys affinis tubers after being cleaned in step S1; a well-ventilated indoor space; a clean tray or drying rack; S22. Operating steps: Place the clean tray or drying rack in a well-ventilated indoor space; Evenly place the cleaned Stachys affinis tubers on the tray or drying rack, and there should be a spacing between the tubers; Control the room temperature at 20 - 25 °C and the relative humidity at 40% - 60%; Let the Stachys affinis tubers air-dry naturally at room temperature for 2 - 3 h; and regularly turn the tubers to evenly dissipate the surface moisture, turning them every 30 min.

4. A method for inhibiting the sprouting of Stachys affinis tubers and prolonging their shelf life according to claim 3, characterized in that, S3. The specific steps of immersing the Stachys affinis tubers in step S2 in a hydrogen peroxide solution with a concentration of 1.0 - 2.5 mg / L for 30 min: S31. Prepare tools and materials: The Stachys affinis tubers with the surface moisture air-dried in step S2; a hydrogen peroxide solution with a concentration of 1.0 - 2.5 mg / L; a container for holding the Stachys affinis tubers, including a plastic basin or a glass tank; S32. Operating steps: Prepare the corresponding amount of hydrogen peroxide solution according to the number of Stachys affinis tubers; For 10 kg of Stachys affinis tubers, according to the volume ratio of tubers to solution of 1:2, 20 L of hydrogen peroxide solution is required; Pour the prepared hydrogen peroxide solution into the container; Put the Stachys affinis tubers with the surface moisture air-dried into the hydrogen peroxide solution and ensure that the tubers are completely immersed in the solution; soak for 30 min and stir the solution to make the tubers fully contact with the solution.

5. A method for inhibiting the sprouting of Stachys affinis tubers and extending their shelf life according to claim 4, characterized in that, Step S4. Air-drying the surface of the Stachys affinis tubers at room temperature includes the following steps: S41. Prepare tools and materials: The Stachys affinis tubers soaked in hydrogen peroxide solution in step S3; a well-ventilated indoor space; a clean tray or drying rack; S42. Operating steps: Place a clean tray or drying rack in a well-ventilated indoor space; evenly arrange the Stachys affinis tubers soaked in hydrogen peroxide solution on the tray or drying rack, and there should be a spacing between the tubers; control the room temperature at 20 - 25 °C and the relative humidity at 40% - 60%.

6. A method for inhibiting the sprouting of Stachys affinis tubers and prolonging their shelf life according to claim 5, characterized in that, S42. Let the Stachys affinis tubers dry naturally at room temperature for 1 - 2 hours; and turn the tubers regularly, turning them every 20 minutes.

7. A method for inhibiting the sprouting of Stachys affinis tubers and extending their shelf life according to claim 6, characterized in that, Step S5. The storage of Stachys affinis at room temperature specifically includes the following steps: Step S51. Put the dried Stachys affinis tubers into non-woven bags one by one, with each bag containing 3.5 kilograms of Stachys affinis tubers; after bagging, use a rope to seal the bag and leave a gap. Step S52. Place the bags containing Stachys affinis tubers in the storage room, with a spacing of 10 - 15 cm between the bags.

8. A method for inhibiting the sprouting of Stachys affinis tubers and prolonging their shelf life according to claim 7, characterized in that, Step S52. The temperature of the storage room should be maintained at 12 - 14 °C, and the relative humidity is controlled at 65% - 68%; use a thermometer and a hygrometer for monitoring, and adjust the working status of the air conditioner and humidifier in time if there is a deviation. During the storage process, check the status of the Stachys affinis tubers every 7 - 10 days to see if there are abnormal situations such as sprouting, rotting, or discoloration; when found, take out the problematic tubers in time.

9. A method for inhibiting the sprouting of Stachys affinis tubers and prolonging their shelf life according to any one of claims 1 to 8, characterized in that, Under the room temperature storage conditions, the germination of Stachys affinis tubers is inhibited for more than one week, and the contents of stachyose, raffinose, and verbascose oligosaccharides are maintained without decrease, while maintaining the quality of Stachys affinis during the extended shelf life at room temperature.

10. Use of a method for inhibiting the sprouting of Stachys affinis tubers and prolonging their shelf life according to any one of claims 1 to 8 in the agricultural product wholesale market of Stachys affinis, or the fresh food supermarket of Stachys affinis, or the e-commerce logistics distribution of Stachys affinis, characterized in that, Use this method to process Stachys affinis tubers to extend their shelf life during wholesale sales or logistics transportation.

Images