Dendrobium candidum tablet candy and preparation method thereof

Abstract

The invention provides dendrobium candidum tablet candies and a preparation method thereof, and belongs to the technical field of candies. The dendrobium officinale tablet candy is prepared from the following components in percentage by mass: 0.1-1% of a dendrobium officinale extract, 0.5-5% of Chinese prickly ash powder, 0-1.5% of vitamin C, 0-1% of citric acid, 0-5% of edible glucose, 0.1-1% of sorbitol, 0.1-1% of erythritol, 10-30% of maltodextrin, 0.1-1% of magnesium stearate, 0-10% of water and the balance of edible corn starch. According to the dendrobium candidum tablet candy provided by the invention, the Chinese prickly ash powder is utilized, so that the flavor is improved, microcirculation is promoted, and the immunity is enhanced.

Description

Technical Field

[0001] The invention belongs to the technical field of candies, and particularly relates to a dendrobium officinale tablet candy and a preparation method thereof. Background Art

[0002] Sichuan pepper has a long history of medicinal use. Modern medical research has found that it has multiple effects such as antioxidant, insecticidal, anesthetic, anti-inflammatory, analgesic, lipid-lowering, wrinkle-removing, anti-cancer, wind-removing and dehumidifying. The traditional extraction process of Sichuan pepper, such as steam extraction of Sichuan pepper powder aromatic oil and then leaching with ethyl acetate, has problems such as cumbersome process, high cost, and high loss of active ingredients. At present, new extraction technologies such as supercritical CO2 extraction technology, microwave-assisted extraction, and enzymatic extraction are gradually being applied to the production of Sichuan pepper powder. These technologies have high extraction efficiency, short extraction time, and no organic solvent residue, which can better retain the effective ingredients and flavor substances of Sichuan pepper.

[0003] Immunity, as the body's own defense mechanism, involves the synergistic effects of multiple factors such as immune cells, immune organs and immune molecules. Nowadays, consumers are paying more and more attention to food safety and health, and the demand for foods with specific health functions is also growing. The importance of self-immunity has reached a new height, and the demand for health foods that can enhance immunity, including candies, has become increasingly strong, which has strongly promoted the research and development of related products.

[0004] At present, there are various types of immunity-enhancing health candies on the market, including compressed candies, soft candies, gel candies, etc. However, the research on the preparation of health candies using pepper powder is relatively scarce and still needs to be developed.

[0005] In view of this, the present invention innovatively provides a Dendrobium officinale tablet candy containing pepper powder and a preparation method thereof, aiming to fill the market gap and provide consumers with more healthy choices. Summary of the invention

[0006] The technical problem to be solved by the present invention is to provide a candy made of dendrobium officinale tablets, which utilizes pepper powder to improve flavor, promote microcirculation and enhance immunity, in view of the deficiencies of the prior art.

[0007] In order to solve the above technical problems, the technical solution adopted by the present invention is: a Dendrobium officinale tablet candy, which is made of the following components in percentage by mass: 0.1-1% of Dendrobium officinale extract, 0.5-5% of pepper powder, 0-1.5% of vitamin C, 0-1% of citric acid, 0-5% of edible glucose, 0.1-1% of sorbitol, 0.1-1% of erythritol, 10-30% of maltodextrin, 0.1-1% of magnesium stearate, 0.1-1% of water, and the balance is edible corn starch.

[0008] Optionally, the pepper powder is prepared by the following method: Step 1: Provide pericarp of Chinese prickly ash, crush it to obtain Chinese prickly ash powder; Step 2: Perform supercritical CO2 extraction on the Chinese prickly ash powder. The CO2 flow rate is ≥ 140 m³ / h, and the entrainer is 5% ethanol. First, adjust the extraction pressure to 15 - 20 MPa, the extraction temperature to 45 - 50 °C, maintain for 25 - 35 min, and collect the volatile oil; then raise the extraction pressure to 30 - 35 MPa, the extraction temperature to 45 - 50 °C, maintain for 120 - 180 min, reduce the pressure to collect the extract, and set aside the residue; Step 3: Add edible alcohol to the residue, perform ultrasonic-assisted extraction, and recover ethanol by reduced-pressure distillation to obtain an edible alcohol extract; Step 4: Combine the volatile oil, extract, and edible alcohol extract, add pregelatinized edible corn starch and mix evenly, sieve to obtain Chinese prickly ash powder.

[0009] Optionally, the dosage of the entrainer in Step 2 is 4 - 5 mL / g.

[0010] Optionally, 35 - 40 g of edible alcohol is added to each gram of the residue in Step 3.

[0011] Optionally, the temperature of the ultrasonic-assisted extraction is 20 - 25 °C, and the time is 50 - 60 min.

[0012] Optionally, the ratio of the added mass of the pregelatinized edible corn starch to the combined mass of the volatile oil, extract, and edible alcohol extract is 100:(30 - 40).

[0013] Optionally, the mass percentage of vitamin C is 0.1 - 1.5%.

[0014] Optionally, the mass percentage of citric acid is 0.1 - 1%.

[0015] Optionally, the mass percentage of edible glucose is 0.1 - 5%.

[0016] The present invention also provides a preparation method of Dendrobium officinale compressed candies, including the following steps: Step 1: Mix Dendrobium officinale extract, Chinese prickly ash powder with maltodextrin, moisten with water, stir to form an inclusion compound, dry and sieve to obtain a maltodextrin mixture; Premix vitamin C with part of the edible corn starch to obtain a vitamin C premix; Step 2: Mix edible glucose, sorbitol, erythritol, citric acid and magnesium stearate with the remaining edible corn starch evenly, then sequentially add the maltodextrin mixture and vitamin C premix and mix evenly to obtain a pre-pressed material; Step 3: Press the pre-pressed material into tablets and dry to obtain Dendrobium officinale compressed candies.

[0017] Optionally, the mass ratio of the partial edible corn starch to the remaining edible corn starch is (3-4):1.

[0018] Optionally, the process parameters for tabletting include: using a rotary tablet press with a pressure of 15-25 kN.

[0019] Compared with the prior art, the beneficial effects of the present invention are as follows: Based on the above solution, the present invention provides a Dendrobium officinale tablet candy containing Chinese prickly ash powder, which combines Dendrobium officinale, Chinese prickly ash powder and vitamin C to form an anti-inflammatory, antioxidant and immune regulation synergistic system; and uses maltodextrin to encapsulate Chinese prickly ash powder to reduce the pungent taste and improve the sensory properties; the compound of edible glucose, sorbitol and erythritol is used to improve and regulate the taste; magnesium stearate is both a tablet lubricant and a magnesium supplement, achieving a dual effect. Based on the above component combination, the present invention optimizes the taste of the Dendrobium officinale tablet candy and enhances the immune effect.

[0020] The Dendrobium officinale tablet candy of the present invention fully optimizes the preparation process based on the above components, specifically manifested in: directly encapsulating Chinese prickly ash powder and Dendrobium officinale extract with water and maltodextrin, without the need for additional solvents or equipment, with controllable costs and improved storage stability; premixing vitamin C with edible corn starch, replacing complex processes such as conventional microcapsule coating through physical premixing, reducing oxidation loss while reducing costs; and using the lubricating effect of magnesium stearate to facilitate direct tabletting. The overall process cost is low, which is beneficial to maintaining the activity of the components and convenient for storage.

[0021] The Dendrobium officinale tablet candy of the present invention has excellent sensory quality and at the same time has the effect of enhancing immunity. Detailed implementation mode

[0022] To better understand the present invention, the content of the present invention will be further clearly described below in conjunction with embodiments, but the protection scope of the present invention is not limited to the following embodiments. In the following description, a large number of specific details are given to provide a more thorough understanding of the present invention. However, it is obvious to those skilled in the art that the present invention can be implemented without one or more of these details.

[0023] Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present invention.

[0024] Unless otherwise specified, all raw materials are commercially available products, and unless otherwise specified, they do not contain other components not specifically indicated except for unavoidable impurities.

[0025] The Dendrobium officinale extract is a commercially available product, food grade, 10:1 water-soluble powder, purchased in powder form, and its particle size is equal to 80 mesh.

[0026] Example 1: A Dendrobium officinale pressed candy is made from the following components by mass percentage: 。

[0027] The above-mentioned Chinese prickly ash powder is prepared by the following method: Step 1: Provide Chinese prickly ash pericarp, crush it to obtain Chinese prickly ash powder; Step 2: Perform supercritical CO2 extraction on the Chinese prickly ash powder. The CO2 flow rate is 140 m³ / h, and the entrainer is 5% ethanol. First, adjust the extraction pressure to 18 MPa, the extraction temperature to 48 °C, and maintain for 30 min to collect the volatile oil; then raise the extraction pressure to 32 MPa, the extraction temperature to 48 °C, and maintain for 150 min, and collect the extract under reduced pressure. The residue is reserved for use; Step 3: Add edible alcohol to the residue, perform ultrasonic-assisted extraction, and recover ethanol by vacuum distillation to obtain an edible alcohol extract; Step 4: Combine the volatile oil, the extract, and the edible alcohol extract, add pregelatinized edible corn starch and mix evenly, and sieve to obtain Chinese prickly ash powder.

[0028] In Step 2, the dosage of the entrainer is 4.5 mL / g.

[0029] In Step 3, 38 g of edible alcohol is added to each gram of the residue.

[0030] The temperature of the ultrasonic-assisted extraction is 23 °C, and the time is 55 min.

[0031] The ratio of the added mass of pregelatinized edible corn starch to the combined mass of the volatile oil, the extract, and the edible alcohol extract is 100:35.

[0032] The present invention first realizes the extraction of volatile oil and oleoresin through supercritical CO2 fractional extraction. Under the conditions of an extraction pressure of 15 - 20 MPa and an extraction temperature of 45 - 50 °C, low-polarity volatile oils such as limonene and aromatic alcohol are extracted; under the conditions of an extraction pressure of 30 - 35 MPa, an extraction temperature of 45 - 50 °C, and maintaining for 120 - 180 min, macromolecular oleoresin components such as cannabinoids and phytosterols are extracted; using edible alcohol as a solvent, ultrasonic waves are used to assist in destroying cell walls to release active substances such as flavonoids and polyphenols, and the residual solvent is removed under reduced pressure. Therefore, the Chinese prickly ash powder of the present invention contains various active components, including limonene, aromatic alcohol, cannabinoids, phytosterols, flavonoids, and / or polyphenols.

[0033] The preparation method of the above-mentioned Dendrobium officinale pressed candy includes the following steps: Step 1: Mix the Chinese prickly ash powder with maltodextrin, moisten it with water, stir to form an inclusion compound, dry it and sieve to obtain a maltodextrin mixture; Premix vitamin C with a portion of edible corn starch to obtain a vitamin C premix; Step 2: Mix edible glucose, sorbitol, erythritol, citric acid, and magnesium stearate with the remaining edible corn starch, then sequentially add the maltodextrin mixture and the vitamin C premix and mix well to obtain a pre-pressed material; Step 3: Compress the pre-pressed material into tablets and dry to obtain Dendrobium officinale tablets.

[0034] The mass ratio of the portion of edible corn starch to the remaining edible corn starch is 3.5:1.

[0035] The process parameters for tablet pressing include: using a rotary tablet press, with a pressure of 20 kN, a tablet weight of 1.8 g, and a hardness of 75 N.

[0036] Examples 2 - 5: The difference from Example 1 is that the component ratios in the Dendrobium officinale tablets are as shown in Table 1.

[0037] Table 1 Component ratios of Examples 2 - 5 (mass percentage, wt%) Example 6: The difference from Example 1 is that in the preparation method of a Dendrobium officinale tablet, the mass ratio of the portion of edible corn starch to the remaining edible corn starch is 3:1; the process parameters for tablet pressing include: a pressure of 15 kN, a tablet weight of 1.8 g, and a hardness of 50 N.

[0038] Example 7: The difference from Example 1 is that in the preparation method of a Dendrobium officinale tablet, the mass ratio of the portion of edible corn starch to the remaining edible corn starch is 4:1; the process parameters for tablet pressing include: a pressure of 25 kN, a tablet weight of 1.8 g, and a hardness of 100 N.

[0039] Example 8: The difference from Example 1 is that in the preparation method of a Dendrobium officinale tablet, the mass ratio of the portion of edible corn starch to the remaining edible corn starch is 4:1; the process parameters for tablet pressing include: a pressure of 23 kN, a tablet weight of 1.8 g, and a hardness of 83 N.

[0040] Comparative Example 1: The difference from Example 1 is that edible corn starch is used to replace maltodextrin.

[0041] Comparative Example 2: The difference from Example 1 is that the preparation method of the Dendrobium officinale tablet includes the following steps: Mix all the raw materials and stir well to obtain a pre-pressed material; Compress the pre-pressed material into tablets at a pressure of 20 kN, with a tablet weight of 1.8 g, and dry to obtain the Dendrobium officinale tablet.

[0042] Comparative Example 3: The difference from Example 1 is that the Chinese prickly ash powder is omitted.

[0043] Comparative Example 4: The difference from Example 1 is that the Chinese prickly ash powder is prepared by the following method: Step 1: Provide Chinese prickly ash pericarp, crush it to obtain Chinese prickly ash powder; Step 2: Perform supercritical CO2 extraction on the Chinese prickly ash powder, with a CO2 flow rate of 140 m³ / h, the entrainer being 5% ethanol, the extraction pressure being 32 MPa, the extraction temperature being 48 °C, maintaining for 150 min, reducing the pressure to collect the extract, and leaving the residue for later use; Step 3: Add edible alcohol to the residue, perform ultrasonic-assisted extraction, and recover ethanol by vacuum distillation to obtain an edible alcohol extract; Step 4: Combine the extract and the edible alcohol extract, add pregelatinized edible corn starch and mix evenly, and sieve to obtain Chinese prickly ash powder. Among them: the dosage of the entrainer in Step 2 is 4.5 mL / g. 38 g of edible alcohol is added to each gram of residue in Step 3. The temperature of ultrasonic-assisted extraction is 23 °C, and the time is 55 min. The ratio of the added mass of pregelatinized edible corn starch to the combined mass of the extract and the edible alcohol extract is 100:35.

[0044] Comparative Example 5: The difference from Example 1 is that the Chinese prickly ash powder is prepared by the following method: Step 1: Provide Chinese prickly ash pericarp, crush it to obtain Chinese prickly ash powder; Step 2: Perform supercritical CO2 extraction on the Chinese prickly ash powder, with a CO2 flow rate of 140 m³ / h, the entrainer being 5% ethanol, first adjust the extraction pressure to 18 MPa, the extraction temperature being 48 °C, maintaining for 30 min, and collect the volatile oil; then raise the extraction pressure to 32 MPa, the extraction temperature being 48 °C, maintaining for 150 min, and reduce the pressure to collect the extract; Step 3: Combine the volatile oil and the extract, add pregelatinized edible corn starch and mix evenly, and sieve to obtain Chinese prickly ash powder. Among them: the dosage of the entrainer in Step 2 is 4.5 mL / g. The ratio of the added mass of pregelatinized edible corn starch to the combined mass of the volatile oil and the extract is 100:35.

[0045] Effect evaluation: 1. Sensory evaluation Perform sensory evaluation on the Dendrobium officinale tablet candies of Examples 1-5 and Comparative Examples 1-2 according to GB / T 29605-2013 "Sensory analysis - Guidelines for the control of sensory quality of foods" and GB 17399-2016 "National food safety standard - Candies". The scoring criteria are shown in Table 2, and the evaluation results are shown in Table 3.

[0046] Table 2 Sensory evaluation criteria Table 3 Evaluation results As can be seen from the data in Table 3, the Dendrobium officinale tablet candies of the present invention perform excellently in terms of appearance, taste, color, smell, and texture, with excellent overall quality and a score of over 90 points. This indicates that its ingredients and ratios are reasonable, and the production process is stable and reliable.

[0047] The results of the comparative experiments showed that in Comparative Example 1, when edible corn starch was used to replace maltodextrin, the appearance, color and texture of the product were significantly affected, and the quality was inferior to that of the present invention. In Comparative Example 2, only the single mixing method was adopted, and the produced Dendrobium officinale tablet candies performed far lower than expected in all aspects and could not meet the quality standards of the present invention.

[0048] It can be seen that by reasonably selecting components and optimizing the process, the present invention effectively overcomes the deficiencies in the comparative experiments and ensures the high quality and excellent performance of the Dendrobium officinale tablet candies.

[0049] 2. Evaluation of the effect of enhancing immunity The Dendrobium officinale tablet candies prepared in Example 1 and Comparative Examples 3-5 were ultramicro pulverized and passed through a 1000-mesh sieve for standby.

[0050] The zebrafish embryos at 7-8 hpf (hours post-fertilization) were transferred to a 12-well plate, and 0.003% PTU was added at 24 hpf to inhibit melanin production. A blank control group, a model group, administration groups of ultramicro powder of Dendrobium officinale tablet candies in Example 1 at different concentrations (0.1, 0.5, 1.0 mg / mL), Comparative Group 1, Comparative Group 2 and Comparative Group 3 were set up. There were 15 in each group, and 3 parallel groups were set.

[0051] The blank control group was given 1 mL of embryo medium per well (a solution made with pure water containing 5 mmol / L NaCl, 0.17 mmol / L KCl, 0.4 mmol / L CaCl2, 0.16 mmol / L MgSO4). The model group was given 10 μg / mL lipopolysaccharide per well. The administration groups were given ultramicro powder of Dendrobium officinale tablet candies in Example 1 at different concentrations (denoted as 1-TG) on the basis of the model group. At 48 hpf, the embryos were exposed to an environment containing or not containing ultramicro powder of Dendrobium officinale tablet candies in Example 1 at different concentrations. Comparative Group 1 was added with 1.0 mg / mL of the ultramicro powder of Comparative Example 3 (denoted as 3-TG), Comparative Group 2 was added with 1.0 mg / mL of the ultramicro powder of Comparative Example 4 (denoted as 4-TG), and Comparative Group 3 was added with 1.0 mg / mL of the ultramicro powder of Comparative Example 5 (denoted as 5-TG). The culture medium was changed every 24 hours, and the incubation was carried out at a constant temperature until 72 hpf.

[0052] (1) Determination of neutrophils: Sudan black staining was used. The embryos were fixed overnight with 4% PFA in PBS at room temperature, rinsed in PBS the next day, and incubated in freshly prepared Sudan black solution for 2 h. After the staining was completed, the zebrafish larvae in each group were washed with 70% ethanol until the washing solution was colorless, and the zebrafish larvae were anesthetized with 0.003% MS-222 solution. The stained larvae were observed and photographed using an Olympus upright fluorescence microscope, and the fluorescence intensity of individual zebrafish embryos was quantified using ImageJ software. The results are shown in Table 4.

[0053] (2)Macrophage assay: Add neutral red solution containing 0.003% PTU at 2.5 mg / mL, incubate in the dark at 28.5 °C for 2.5 hours to produce the best macrophage staining effect; after incubation, aspirate the dye, wash the zebrafish larvae in each group with embryo medium until the washing solution is clear, anesthetize the zebrafish larvae with 0.003% MS-222 solution, observe the stained larvae with an Olympus upright fluorescence microscope and take pictures, and use ImageJ software to quantify the fluorescence intensity of individual zebrafish embryos. The results are shown in Table 4.

[0054] Table 4 Results of neutrophil and macrophage assays Note: ### P < 0.001, significantly different from the blank control group, ** P < 0.01 and * P < 0.05, significantly different from the model group.

[0055] Table 4 results show that in the medium-dose administration group compared with the model group, the numbers of neutrophils and macrophages were significantly reduced, and there was a significant difference between the two groups; there was a highly significant difference in the high-dose administration group compared with the model group, indicating that the ultrafine powder of the Dendrobium officinale pressed candy of the present invention can significantly reduce the aggregation of neutrophils and macrophages at the inflammatory site in LPS-stimulated zebrafish, can initiate the innate immune response, help the body resist infection, and enhance the immune ability to a certain extent. The comparison results show that in the high-dose concentration of Comparison Group 1, there was no significant difference compared with the model group; in the high-dose concentration of Comparison Group 2, there was no significant difference compared with the model group; in the high-dose concentration of Comparison Group 3, there was no significant difference compared with the model group. It can be seen that the Dendrobium officinale pressed candy of the present invention has a more significant immune-enhancing effect by combining Dendrobium officinale extract and Chinese prickly ash powder.

[0056] In summary, the Dendrobium officinale pressed candy of the present invention has excellent sensory quality and at the same time has the effect of enhancing immunity.

[0057] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and not to limit them. Any other modifications or equivalent replacements made by those of ordinary skill in the art to the technical solutions of the present invention shall fall within the scope of the claims of the present invention as long as they do not depart from the spirit and scope of the technical solutions of the present invention.

Claims

1. A Dendrobium officinale pressed candy, characterized in that: It is made of the following components by mass percentage: 0.1 - 1% of Dendrobium officinale extract, 0.5 - 5% of Chinese prickly ash powder, 0 - 1.5% of vitamin C, 0 - 1% of citric acid, 0 - 5% of edible glucose, 0.1 - 1% of sorbitol, 0.1 - 1% of erythritol, 10 - 30% of maltodextrin, 0.1 - 1% of magnesium stearate, 0 - 10% of water, and the balance is edible corn starch.

2. The Dendrobium officinale pressed candy according to claim 1, characterized in that: The mass percentage of the vitamin C is 0.1 - 1.5%.

3. The Dendrobium officinale pressed candy according to claim 1, wherein: The mass percentage of the citric acid is 0.1 - 1%.

4. The tablet confectionery of Dendrobium officinale as claimed in claim 1, wherein: The mass percentage of the edible glucose is 0.1 - 5%.

5. The preparation method of a Dendrobium officinale pressed candy according to any one of claims 1-4, characterized in that: It includes the following steps: Step 1: Mix the Dendrobium officinale extract, Chinese prickly ash powder and maltodextrin, moisten with water, stir to form an inclusion complex, sieve after drying to obtain a maltodextrin mixture; Premix the vitamin C with part of the edible corn starch to obtain a vitamin C premix; Step 2: Mix the edible glucose, sorbitol, erythritol, citric acid and magnesium stearate with the remaining edible corn starch, then sequentially add the maltodextrin mixture and the vitamin C premix and mix evenly to obtain a pre-pressed material; Step 3: Press the pre-pressed material into tablets and dry to obtain Dendrobium officinale tablet candies.

6. The tablet candy of Dendrobium officinale as claimed in claim 5, wherein: The mass ratio of the part of the edible corn starch to the remaining edible corn starch is (3 - 4):

1.

7. The tablet candy of Dendrobium officinale as claimed in claim 5, wherein: The process parameters for tablet pressing include: using a rotary tablet press with a pressure of 15 - 25 kN.

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