Preparation method of semen persicae herbal eleven-ingredient solid beverage

Abstract

The invention discloses a preparation method of a peach kernel herbal eleven-ingredient solid beverage, which takes eleven traditional Chinese medicine compounds such as peach kernel, cordate houttuynia, purslane and the like as a core, and the effects of clearing away heat and toxic materials, resisting inflammation and moistening lung are synergistically enhanced through compatibility. The extraction, granulation and packaging processes of the medicinal materials are further optimized, and the problems of loss of active ingredients, moisture absorption and caking of particles and microbe control are solved. The industrial stable production in a 300,000-grade clean workshop is realized, and the GB 7101-2022 national standard beverage requirements for food safety are met.

Description

Technical Field

[0001] The present invention belongs to the technical fields of food processing and traditional Chinese medicine compound, and relates to a functional solid beverage with a specific traditional Chinese medicine ratio as the core and a preparation method thereof, specifically a preparation method of a solid beverage of eleven herbs with peach kernels. Background Art

[0002] With the improvement of people's living standards and the continuous strengthening of the concept of healthy consumption, foods with health care functions are increasingly favored by people. Solid beverages have the characteristics of being natural, green, functional and health care, and convenient to carry, and are increasingly loved by consumers. Especially the solid beverages of medicated and edible homologous substances, which not only have the advantages of solid beverages, but also have the characteristics of medicated and edible homologous foods for diet therapy or adjuvant medical treatment. The solid beverages of medicated and edible homologous substances greatly meet the modern people's pursuit of returning to nature and using food as medicine.

[0003] However, traditional solid beverages have the following problems: 1. In the traditional extraction and concentration process, traditional Chinese herbal medicines usually use long-term decoction at one time to extract active ingredients during decoction extraction. Ordinary heating concentration methods are operated under normal pressure and relatively high temperatures, resulting in partial loss of active ingredients; 2. The traditional trough-type mixer has low efficiency and poor mixing uniformity when mixing particles. Although methods such as spray drying or energy-saving drying are widely used, they will cause thermal sensitivity loss of components, and the particle size control is not fine enough. The inspection of particle shape and size often depends on manual work, with low efficiency and easy to produce errors; 3. Insufficient stability: The extract powder has strong hygroscopicity, is easy to agglomerate after granulation, and the quality drops significantly during the shelf life; 4. Hygiene risk: The cleanliness of ordinary production workshops is insufficient (usually at the ordinary level), resulting in a high risk of microbial over-standard.

[0004] Therefore, there is an urgent need for a technical solution that takes into account the scientificity of the formula, the efficiency of the process and the safety of production. Summary of the Invention

[0005] The purpose of the present invention is to provide a preparation method of a solid beverage of eleven herbs with peach kernels, so as to solve the problems of low extraction efficiency of medicinal components, easy moisture absorption of products and insufficient hygiene control in the production process of existing solid beverages.

[0006] A preparation method of a solid beverage of eleven herbs with peach kernels of the present invention includes the following raw materials and processes: Step 1: Pretreatment of raw materials First, detect the moisture, heavy metals and pesticide residues of each raw material according to the standards of the Chinese Pharmacopoeia (2020 Edition) to ensure that the raw materials meet the standards. Subsequently, remove the mildewed and insect-eaten particles of each raw material through a color sorter to ensure that the impurity content is ≤ 0.5%; The above raw material formula is in mass percentage: Houttuynia cordata 14%, Portulaca oleracea 13%, Prunus persica 13%, Gardenia jasminoides 9%, Morus alba leaf 9%, Coix lacryma-jobi 8%, Perilla frutescens 8%, Mentha haplocalyx 8%, Ginkgo biloba seed 6%, Prunus mume 6%, Cassia obtusifolia 6%. As a whole, it focuses on clearing heat and promoting diuresis, detoxifying and resolving phlegm, and also takes into account activating blood circulation and dredging collaterals, as well as regulating qi movement. The following analyzes its efficacy principle from the perspective of monarch, minister, assistant, and courier herbs: Analysis of the compatibility of monarch, minister, assistant, and courier herbs: Monarch herbs: Houttuynia cordata (14%), Portulaca oleracea (13%) Functions: Houttuynia cordata clears heat and detoxifies, reduces swelling and dissipates nodules, promotes diuresis and relieves stranguria. Modern research shows that it contains quercetin and flavonoid compounds, which can enhance immunity, anti-inflammatory and antibacterial; Portulaca oleracea cools blood and stops bleeding, relieves dysentery and inflammation, and contains norepinephrine to assist in reducing blood sugar and blood pressure.

[0007] Significance of compatibility: The two herbs have the highest proportion (a total of 27%). For the main symptoms of damp-heat accumulation and phlegm-fire congestion, they work together to clear heat and promote diuresis, and detoxify and expel pus.

[0008] 2. Minister herbs: Prunus persica (13%), Gardenia jasminoides (9%), Morus alba leaf (9%), Coix lacryma-jobi (8%) Prunus persica: Promotes blood circulation to remove stasis, loosens the bowels and relieves constipation, helps the monarch herbs resolve damp-heat stasis obstruction, and improves blood stasis type phlegm-dampness.

[0009] Gardenia jasminoides: Clears away fire and relieves vexation, clears heat and promotes diuresis, clears heat stagnation in the triple energizer, especially good at treating damp-heat in the liver and gallbladder.

[0010] Morus alba leaf: Disperses wind-heat, clears the liver and improves eyesight, assists in clearing lung heat and regulating liver qi.

[0011] Coix lacryma-jobi: Strengthens the spleen and promotes diuresis, detoxifies and dissipates nodules, removes the existing dampness and prevents the regeneration of damp pathogen.

[0012] Significance of compatibility: The minister herbs account for 39% in total, enhancing the power of the monarch herbs in clearing heat and promoting diuresis, and at the same time taking into account promoting blood circulation, strengthening the spleen, and soothing the liver.

[0013] 3. Assistant herbs: Perilla frutescens (8%), Mentha haplocalyx (8%), Ginkgo biloba seed (6%), Prunus mume (6%) Perilla frutescens: Regulates qi and soothes the middle, relieves exterior syndrome and dispels cold, regulates qi stagnation in the spleen and stomach, and prevents the cold-natured herbs from damaging the stomach.

[0014] Mentha haplocalyx: Soothes the liver and promotes qi movement, clears the head and eyes, helps the qi movement to be smooth, and also guides the herbs upward.

[0015] Ginkgo biloba seed: Astringes the lung and relieves asthma, stops leukorrhea and reduces urination, astringes the lung qi to prevent excessive dispersion.

[0016] Prunus mume: Promotes the production of body fluid and quenches thirst, astringes the intestine and expels roundworms, relieves the injury of body fluid caused by damp-heat, and coordinates the sour astringency and pungent dispersion.

[0017] Significance of compatibility: The assistant herbs account for 28%, regulating the bias of the properties of the monarch and minister herbs, and taking into account the rise and fall of qi movement and the balance of body fluid.

[0018] 4. Medicinal herb: Cassia seed (6%) Functions: Clearing heat and improving eyesight, moistening the intestines and promoting defecation, guiding damp-heat to be excreted through urine and feces, and concurrently treating red and swollen eyes.

[0019] Significance of compatibility: Accounting for 6%, it plays a guiding role, enabling the medicinal power of the whole formula to concentrate on the liver, gallbladder, spleen, stomach and intestines.

[0020] Step 2: Water extraction of raw materials Three times of water extraction are adopted. Each time, the water addition amount is 6 - 10 times the total weight of the raw materials, the extraction temperature is 80 - 90 °C, and each time is 1 - 1.5 hours. The filtrates of the three times are combined to obtain the extraction solution; The specific extraction process is as follows: The first extraction: The water addition amount is 10 times the total weight of the raw materials, the temperature is 85 °C, and the time is 1.5 h; The second extraction: The water addition amount is 8 times the total weight of the raw materials, the temperature is 90 °C, and the time is 1 h; The third extraction: The water addition amount is 6 times the total weight of the raw materials, the temperature is 80 °C, and the time is 1 h; After the filtrates of the three times are combined, they are filtered through a 200 - mesh sieve to obtain the extraction solution.

[0021] Step 3: Ultrafiltration - nanofiltration refinement An ultrafiltration - nanofiltration combined device is used to refine the extraction solution; The ultrafiltration - nanofiltration combined device includes an extraction solution storage tank, a constant temperature water bath tank and a membrane module. The extraction solution storage tank, the constant temperature water bath tank and the membrane module are connected in sequence to form a circulation loop. A temperature sensor, a first pressure sensor and a first pressure regulating valve are installed on the connecting pipeline between the constant temperature water bath tank and the membrane module. A rotational speed regulator, a second pressure sensor and a second regulating valve are installed on the connecting pipeline between the membrane module and the extraction solution storage tank; The filter membrane inside the membrane module can be detachably replaced with an ultrafiltration membrane or a nanofiltration membrane, and a filtrate storage tank is also connected to the membrane module; The refinement process includes the following steps: 1) Place the extraction solution in the extraction solution storage tank, after being treated at a constant temperature in a constant temperature reaction kettle, pump it to the membrane module for ultrafiltration treatment, and then return it to the extraction solution storage tank. The whole process is in a circulating state, and finally, the ultrafiltration refined solution is collected from the filtrate storage tank; During ultrafiltration treatment, the filter membrane inside the membrane module is replaced with an ultrafiltration membrane. The ultrafiltration pressure range is 0.08 - 0.12 MPa, the temperature range is 25 - 40 °C, the cross - flow velocity range on the membrane surface is 3.3 - 5.3 m / s. When the ratio of the permeate to the retentate is 6 - 7:1, stop ultrafiltration; 2) Place the ultrafiltration refined liquid in the extraction liquid storage tank. After being treated at a constant temperature in a constant temperature reaction kettle, it is pumped to a membrane module for nanofiltration treatment, and then returned to the extraction liquid storage tank. The whole process is in a cyclic state. Finally, the nanofiltration refined liquid is collected from the filtrate storage tank. During nanofiltration treatment, the nanofiltration membrane is replaced inside the membrane module. The nanofiltration conditions are an operating pressure of 0.16 - 0.20 MPa, a temperature of 25 - 40 °C, a cross-flow velocity of the membrane surface of 4.5 - 6.5 m / s. Stop nanofiltration when the ratio of the retained liquid to the permeate is 5 - 6:1. Step 4: Low-temperature vacuum concentration Take the nanofiltration refined liquid and concentrate it under reduced pressure to make a paste. Concentration temperature: 60 - 65 °C, vacuum degree -0.08 MPa, concentrate to a relative density of 1.12 ± 0.02 g / mL. Step 5: Vacuum drying to make powder Dry the extract with a relative density of 1.25 after concentration under the common process conditions of vacuum drying, at a temperature of 60 °C and a vacuum degree of -0.09 MPa. The dried extract obtained is pulverized and passed through an 80-mesh sieve to obtain the dried extract powder. Step 6: Mixing and granulation Addition of excipients: Take maltodextrin and xylitol in a mass ratio of (2 - 3):1, mix them, and then mix the mixture with the extract powder in a mass ratio of (3 - 5):1. Granulation: Use a swing granulator, granulation time 10 - 20 min, particle size distribution 0.3 - 0.8 mm. Drying: Use fluidized bed drying, inlet air temperature 50 - 60 °C, drying time 30 - 40 min, moisture content of the final product ≤ 6.5%. Step 7: Clean packaging Inner packaging: Use an aluminum foil bag for packaging, oxygen transmission rate < 0.5 cc / m 2 ·day, and seal it with nitrogen in a 300,000-class clean workshop. Outer packaging: A composite film bag, sterilize it with ultraviolet light after labeling, wavelength 254 nm, irradiation dose 800 - 1000 μW·s / cm 2 .

[0022] Compared with the prior art, the present invention has the following beneficial effects: 1. High-efficiency extraction: The extraction rate of the total active ingredients > 90%, which is 10 - 15% higher than the traditional process. Compared with the water extraction and alcohol precipitation method, the most widely used refined process in current traditional Chinese medicine extraction, ceramic membrane ultrafiltration can retain the nutritional components and active ingredients in the original medicinal liquid to the greatest extent, and nanofiltration can further remove water and inorganic salts and concentrate the active ingredients. Applying the two simultaneously to the separation and purification of traditional Chinese medicine extraction liquid has many advantages such as simple operation, short production cycle, energy conservation and environmental protection.

[0023] 2. Excellent stability: No caking after 3 months of accelerated test, and the moisture fluctuation is <1%; 3. Meeting safety standards: Microbiological indicators comply with GB 7101-2022, and no artificial preservatives are added; 4. Industrial applicability: Continuous production can be achieved in a 300,000-class clean workshop throughout the process, and the daily production capacity is ≥1 ton. Description of the Drawings

[0024] Figure 1 It is the process flow chart of the preparation of the solid beverage of the present invention.

[0025] Figure 2 It is the structural schematic diagram of the ultrafiltration-nanofiltration combined equipment used in the present invention.

[0026] In the figure, 1 - extraction liquid storage tank, 2 - constant temperature water bath, 3 - membrane module, 4 - temperature sensor, 5 - first pressure sensor, 6 - first regulating valve, 7 - second pressure sensor, 8 - second regulating valve, 9 - speed regulator, 10 - filtrate storage tank. Detailed Embodiments

[0027] The present invention will be further explained below with reference to the drawings and specific embodiments.

[0028] Example 1 Step 1: Pretreatment of raw materials First, detect the moisture, heavy metals, and agricultural residues of each raw material according to the standards of the 2020 edition of the Chinese Pharmacopoeia to ensure that the raw materials meet the standards. Subsequently, remove the mildewed and insect-eaten particles of each raw material through a color sorter to ensure that the impurity content is ≤0.5%; The above raw material formula is in terms of mass percentage, Houttuynia cordata 14%, Portulaca oleracea 13%, Prunus persica 13%, Gardenia jasminoides 9%, Morus alba 9%, Coix lacryma-jobi 8%, Perilla frutescens 8%, Mentha haplocalyx 8%, Ginkgo biloba 6%, Prunus mume 6%, Cassia obtusifolia 6%; The whole takes clearing heat and promoting diuresis, detoxifying and resolving phlegm as the core, and also takes into account promoting blood circulation and dredging collaterals and regulating qi movement. The following analyzes its efficacy principle from the perspective of monarch, minister, assistant, and envoy: Analysis of the compatibility of monarch, minister, assistant, and envoy: Monarch drugs: Houttuynia cordata (14%), Portulaca oleracea (13%) Functions: Houttuynia cordata clears heat and detoxifies, reduces swelling and dissipates nodules, and promotes diuresis and relieves stranguria. Modern research shows that it contains quercetin and flavonoid compounds, which can enhance immunity, anti-inflammatory and antibacterial; Portulaca oleracea cools blood and stops bleeding, relieves dysentery and inflammation, and contains norepinephrine to assist in lowering blood sugar and blood pressure.

[0029] Significance of compatibility: The two account for the highest proportion (a total of 27%), targeting the main syndromes of damp-heat accumulation and phlegm-fire congestion, and jointly achieving the effects of clearing heat and promoting diuresis, detoxifying and expelling pus.

[0030] 2. Minister drugs: Prunus persica (13%), Gardenia jasminoides (9%), Morus alba (9%), Coix lacryma-jobi (8%) Peach kernel: Promote blood circulation to remove stasis, moisten the intestines and relieve constipation, assist the monarch drug to resolve damp-heat stasis obstruction, and improve blood stasis type phlegm-dampness.

[0031] Gardenia: Reduce pathogenic fire and relieve restlessness, clear heat and promote diuresis, clear the stagnant heat of the triple energizer, especially good at treating damp-heat in the liver and gallbladder.

[0032] Mulberry leaf: Disperse wind-heat, clear the liver and improve eyesight, assist in clearing lung heat and regulating liver qi.

[0033] Coix seed: Strengthen the spleen and promote diuresis, detoxify and dissipate nodules, remove the existing dampness and prevent the regeneration of damp pathogen.

[0034] Compatibility significance: The ministerial drugs account for 39% in total, enhancing the power of the monarch drug in clearing heat and promoting diuresis, and at the same time taking into account promoting blood circulation, strengthening the spleen and soothing the liver.

[0035] 3. Assistant drugs: Perilla (8%), Mentha haplocalyx (8%), Ginkgo biloba (6%), Fructus Mume (6%) Perilla: Regulate qi and relieve distension, relieve exterior syndrome and dispel cold, regulate qi stagnation in the spleen and stomach, and prevent the cold-natured drugs from damaging the stomach.

[0036] Mentha haplocalyx: Soothe the liver and promote qi movement, clear the head and eyes, assist the qi movement to be smooth, and also guide the drugs upward.

[0037] Ginkgo biloba: Astringe the lung and relieve asthma, arrest leucorrhea and reduce urination, astringe the lung qi to prevent excessive dispersion.

[0038] Fructus Mume: Promote the production of body fluid and quench thirst, astringe the intestine and expel ascaris, relieve the injury of body fluid caused by damp-heat, and coordinate the sour astringency and pungent dispersion.

[0039] Compatibility significance: The assistant drugs account for 28%, coordinate the bias of the properties of the monarch and ministerial drugs, and take into account the rise and fall of qi movement and the balance of body fluid.

[0040] 4. Guiding drug: Cassia seed (6%) Function: Clear heat and improve eyesight, moisten the intestines and relieve constipation, guide the damp-heat to be excreted through the urine and feces, and concurrently treat red and swollen eyes.

[0041] Compatibility significance: Accounting for 6%, playing the role of guiding the meridian, making the medicinal power of the whole prescription concentrate on the liver, gallbladder, spleen, stomach and intestines.

[0042] Step 2: Water extraction of raw materials The above raw material formula is subjected to water extraction by a three-time water extraction process, specifically as follows: The first extraction: The amount of added water is 10 times the total weight of the raw materials, the temperature is 85°C, and the time is 1.5 h; The second extraction: The amount of added water is 8 times the total weight of the raw materials, the temperature is 90°C, and the time is 1 h; The third extraction: The amount of added water is 6 times the total weight of the raw materials, the temperature is 80°C, and the time is 1 h; The three filtrates are combined and filtered through a 200-mesh sieve to obtain 120 L of extraction solution.

[0043] Step 3: Ultrafiltration - nanofiltration refinement As Figure 2 shown, the ultrafiltration - nanofiltration system combination equipment of the present invention includes an extraction liquid storage tank 1, a constant temperature water bath 2, and a membrane module 3. The extraction liquid storage tank 1, the constant temperature water bath 2, and the membrane module 3 are connected in sequence to form a circulation loop. A delivery pump (not shown in the figure), a temperature sensor 4, a first pressure sensor 5, and a first pressure regulating valve 6 are installed on the connecting pipeline between the constant temperature water bath 2 and the membrane module 3. A second pressure sensor 7, a second regulating valve 8, and a rotational speed regulator 9 are installed on the connecting pipeline between the membrane module and the extraction liquid storage tank; the filter membrane inside the membrane module 3 can be detachably replaced with an ultrafiltration membrane or a nanofiltration membrane, and a filtrate storage tank 10 is also connected to the membrane module 3.

[0044] Among them, the temperature sensor 4 is used to monitor the temperature of the extraction liquid entering the membrane module to timely adjust the temperature of the constant temperature water bath; the first pressure sensor 5 and the second pressure sensor 7 are used to monitor the working pressure of the membrane module to determine whether the membrane module is operating normally; the first regulating valve 6 and the second regulating valve 8 are used to adjust the working pressure of the membrane module to meet the ultrafiltration / nanofiltration requirements; the rotational speed regulator 9 is used to regulate the flow rate and control the cross - flow velocity of the membrane surface.

[0045] Using the above - mentioned equipment to carry out ultrafiltration - nanofiltration purification and refinement on 120 L of extraction liquid, and finally obtaining 30 L of nanofiltration refined liquid; the specific steps are as follows: 1) Place the extraction liquid in the extraction liquid storage tank, after being thermostatically treated by a constant temperature reaction kettle, pump it to the membrane module for ultrafiltration treatment, and then return it to the extraction liquid storage tank. The whole process is in a circulating state, and finally collect the ultrafiltrate from the filtrate storage tank; the ultrafiltration process can select an inorganic ceramic membrane with a pore size of 50 nm as needed to achieve molecular - level separation of the water - extracted liquid. Using the orthogonal experimental method, the optimal process parameters are determined as follows: the ultrafiltration pressure range is 0.12 MPa, the temperature range is 25 °C, the cross - flow velocity range of the membrane surface is 5.3 m / s. When the ratio of the permeate to the retentate is 7:1, stop ultrafiltration; 3) Place the ultrafiltrate in the extraction liquid storage tank, after being thermostatically treated by a constant temperature reaction kettle, pump it to the membrane module for nanofiltration treatment, and then return it to the extraction liquid storage tank. The whole process is in a circulating state, and finally collect the nanofiltrate from the filtrate storage tank; the nanofiltration process can remove some water and inorganic salt ions, and can further concentrate and refine the astragalus ultrafiltrate. An organic membrane with a molecular weight cut - off of 10 kDa for the nanofiltration membrane is used. Using the orthogonal experimental method, the optimal process parameters are determined as follows: the nanofiltration pressure is 0.16 MPa, the temperature is 40 °C, the cross - flow velocity of the membrane surface is 4.5 m / s. When the ratio of the retentate to the permeate is 6:1, stop nanofiltration.

[0046] Step 4: Low - temperature vacuum concentration Take the nanofiltration refined liquid and concentrate it under reduced pressure to make a paste; Concentration temperature: 60 - 65 °C, vacuum degree - 0.08 MPa, concentrate to a relative density of 1.12 ± 0.02 g / mL; Step 5: Vacuum drying for powder preparation The concentrated extract with a relative density of 1.25 is dried under the common process conditions of vacuum drying, at a temperature of 60°C and a vacuum degree of -0.09 MPa. The obtained dry extract is pulverized and then passed through an 80-mesh sieve to obtain dry extract powder.

[0047] Step 6: Mixing and granulation Addition of excipients: Take 2.1 kg of maltodextrin and 0.7 kg of xylitol and mix them, then mix the mixture with 8.5 kg of extract powder according to the mass ratio; Granulation: Use a 40-mesh sieve swing granulator, with a granulation time of 10 min, and the particle size distribution of the granules is 0.3 - 0.8 mm; Drying: Use fluidized bed drying, with an inlet air temperature of 55°C, a drying time of 30 min, and the moisture content of the final product ≤ 6.5%; Step 7: Clean packaging After granulation and drying, it is packaged into 3 g / bags: Inner packaging: Use aluminum foil bags for packaging, with an oxygen transmission rate < 0.5 cc / m 2 ·day, and nitrogen is filled and sealed in a clean workshop of 300,000 - grade; Outer packaging: Composite film bags, after labeling, sterilized by ultraviolet light, with a wavelength of 254 nm and an irradiation dose of 800 - 1000 μW·s / cm 2 .

[0048] Example 2 Accelerated stability test To evaluate the degradation trend of the drug under short-term extreme conditions, at least 3 batches of the finished packaged solid beverages produced normally in Example 1 are taken for testing respectively. According to the standard accelerated conditions set by ICH Q1A(R2), 40 ± 2°C, relative humidity (RH) 75% ± 5%, for 6 months. The results show that: stored at 40°C / RH 75% for 6 months, there is no caking, the moisture fluctuation < 1%, the retention rate of flavonoids > 90%, and the microbial indicators meet GB 7101 - 2022.

[0049] Example 3 In vitro antiviral experiment The effects of the finished packaged solid beverage on the cytopathic effect caused by influenza A H1N1 virus were investigated by using the cytopathic effect (CPE) and MTT assay. After the dog kidney cell line (MDCK cells) and human laryngeal carcinoma epithelial cell line (Hep-2 cells) were revived and their states were adjusted, they were inoculated into 96-well cell culture plates at concentrations of 1.5×105 cells / mL and 2.0×105 cells / mL. According to the virus infection process, three experimental groups were designed: drug prevention group (A), replication inhibition group (B), and direct killing group (C). Among them, the virus inoculation concentration was 100 TCID50, and the test medicinal virus growth solution was diluted 5 concentrations downward from TC0. During the experiment, the cytopathic effect CPE was observed regularly under an inverted microscope, and finally the MTT assay was used to detect the activity of the virus-infected cells and calculate the cell survival rate.

[0050] The experimentally measured maximum non-toxic concentrations (TCO) of the solid beverage on the dog kidney cell line (MDCK cells) and human laryngeal carcinoma epithelial cell line (Hep-2 cells) were 3.12 mg / mL and 4.11 mg / mL, respectively. The median infective dose TCID of influenza A H1N1 virus on MDCK cells and Hep-2 cells 50 was 10 -4.5 and 10 -3.6 . Among the three action modes in this experiment, the survival rates of the virus-infected cells in each concentration group in the replication inhibition group were higher. Among them, the survival rate of MDCK cells increased by up to 61.6% when the concentration of the solid beverage was 3 mg / mL; the survival rate of Hep-2 cells increased by up to 40.6% when the concentration of the solid beverage was 4 mg / mL. The results of the experimental group were significantly different from those of the drug blank group in terms of cell survival rate (p<0.05).

Claims

1. A method for preparing a solid beverage of eleven herbs with peach kernels, comprising the following raw materials and processes: Raw materials: Calculated by mass percentage, Houttuynia cordata 14%, Portulaca oleracea 13%, peach kernels 13%, Gardenia jasminoides 9%, Morus alba leaves 9%, Coix lacryma-jobi 8%, Perilla frutescens 8%, Mentha haplocalyx 8%, Ginkgo biloba seeds 6%, Prunus mume 6%, Cassia obtusifolia 6%; The process includes the following steps: Step 1: Pretreatment of raw materials First, detect the moisture, heavy metals, and pesticide residues of each raw material according to the standards of the 2020 edition of the Chinese Pharmacopoeia to ensure that the raw materials meet the standards. Subsequently, use a color sorter to remove mildewed and insect-eaten particles from each raw material to ensure that the impurity content is ≤0.5%; Step 2: Water extraction of raw materials Perform water extraction three times, with the water addition amount each time being 6 - 10 times the total weight of the raw materials, the extraction temperature being 80 - 90°C, and each time for 1 - 1.5 hours. Combine the filtrates of the three times to obtain an extract; Step 3: Ultrafiltration - nanofiltration refinement Use an ultrafiltration - nanofiltration combined device to refine the extract; The ultrafiltration - nanofiltration combined device includes an extract storage tank, a constant temperature water bath, and a membrane module. The extract storage tank, the constant temperature water bath, and the membrane module are connected in sequence to form a circulation loop. A temperature sensor, a first pressure sensor, and a first pressure regulating valve are installed on the connecting pipeline between the constant temperature water bath and the membrane module. A speed regulator, a second pressure sensor, and a second regulating valve are installed on the connecting pipeline between the membrane module and the extract storage tank; The filter membrane inside the membrane module can be detachably replaced with an ultrafiltration membrane or a nanofiltration membrane, and a filtrate storage tank is also connected to the membrane module; The refinement process includes the following steps: 1) Place the extract in the extract storage tank, perform constant temperature treatment in a constant temperature reaction kettle, then pump it to the membrane module for ultrafiltration treatment, and then return it to the extract storage tank. The whole process is in a circulating state, and finally, collect the ultrafiltration refined liquid from the filtrate storage tank; 2) Place the ultrafiltration refined liquid in the extract storage tank, perform constant temperature treatment in a constant temperature reaction kettle, then pump it to the membrane module for nanofiltration treatment, and then return it to the extract storage tank. The whole process is in a circulating state, and finally, collect the nanofiltration refined liquid from the filtrate storage tank; Step 4: Low-temperature vacuum concentration Take the nanofiltration refined liquid and concentrate it under reduced pressure to make a paste; Concentration temperature: 60 - 65°C, vacuum degree -0.08 MPa, concentrate to a relative density of 1.12 ± 0.02 g / mL; Step 5: Vacuum drying to make powder Dry the concentrated extract with a relative density of 1.25 under the common process conditions of vacuum drying, at a temperature of 60°C and a vacuum degree of -0.09 MPa; The dried dry extract is pulverized and passed through an 80-mesh sieve to obtain dry extract powder; Step 6: Mixing and granulation Addition of excipients: Take maltodextrin and xylitol and mix them in a mass ratio of (2 - 3):1, and then mix the mixture with the extract powder in a mass ratio of (3 - 5):1; Granulation: Use a swing granulator, granulation time 10 - 20 min, particle size distribution 0.3 - 0.8 mm; Drying: Use fluidized bed drying, inlet air temperature 50 - 60°C, drying time 30 - 40 min, final product moisture ≤6.5%; Step 7: Clean packaging Inner packaging: Packed in aluminum foil bags with an oxygen transmission rate of <0.5cc / m 2 ·day, nitrogen-filled and sealed in a clean workshop of 300,000-class cleanliness; Outer packaging: Composite film bag, sterilized by ultraviolet light after labeling, wavelength 254 nm, irradiation dose 800 - 1000 μW·s / cm 2 .

2. The preparation method of an eleven-herb solid beverage with peach kernels as claimed in claim 1, characterized in that, In step 2), perform water extraction three times to fully extract the raw materials, specifically as follows: First extraction: The water addition amount is 10 times the total weight of the raw materials, the temperature is 85°C, and the time is 1.5 h; Second extraction: The amount of water added is 8 times the total weight of the raw materials, the temperature is 90 °C, and the time is 1 h; Third extraction: The amount of water added is 6 times the total weight of the raw materials, the temperature is 80 °C, and the time is 1 h; The three filtrates are combined and filtered through a 200-mesh sieve to obtain the extract.

3. The preparation method of an eleven-herb solid beverage with peach kernels as claimed in claim 1, characterized in that, In step 3), during the ultrafiltration treatment, the inside of the membrane module is replaced with an ultrafiltration membrane. The ultrafiltration pressure range is 0.08 - 0.12 MPa, the temperature range is 25 - 40 °C, the cross-flow velocity on the membrane surface ranges from 3.3 to 5.3 m / s. When the ratio of the permeate to the retentate is 6 - 7:1, stop ultrafiltration.

4. The preparation method of an eleven-herb solid beverage with peach kernels as claimed in claim 1, wherein In step 3), during the nanofiltration treatment, the inside of the membrane module is replaced with a nanofiltration membrane. The nanofiltration conditions are an operating pressure of 0.16 - 0.20 MPa, a temperature of 25 - 40 °C, a cross-flow velocity on the membrane surface of 4.5 - 6.5 m / s. When the ratio of the retentate to the permeate is 5 - 6:1, stop nanofiltration.

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