A firming and anti-wrinkle composition and its application

Abstract

The present invention discloses an adenosine firming and anti-wrinkle composition and its application, specifically belonging to the field of cosmetic preparations; the composition comprises: adenosine, sunflower sprout extract, hydrolyzed pansy extract, and Artemia extract; the firming and anti-wrinkle composition provided by the present invention can effectively inhibit elastase synthesis and has excellent firming and anti-wrinkle effects; the firming and anti-wrinkle essence containing the firming and anti-wrinkle composition provided by the present invention has excellent firming and anti-wrinkle effects.

Description

Technical Field

[0001] The invention belongs to the field of cosmetic preparations, and particularly relates to a firming and anti-wrinkle composition and application thereof. Background Art

[0002] In the field of anti-aging skin care, elastin network degradation and collagen loss are the core mechanisms that lead to skin sagging and wrinkle formation. Current anti-wrinkle products mostly focus on a single mechanism of action: either promoting fibroblast energy metabolism through nucleotides such as adenosine, or using plant extracts to inhibit the activity of matrix metalloproteinases. However, existing technologies have significant limitations: (1) The target of a single ingredient is limited. For example, although conventional adenosine formulas can promote collagen synthesis, they cannot block the degradation of elastic fibers by elastase; (2) The synergistic effect of ingredients is insufficient. Most products adopt a simple superposition strategy and fail to form a multi-dimensional protection network of "synthesis promotion-degradation inhibition-structural repair"; (3) The influence of the hydration microenvironment is ignored. Water loss in the dermis will accelerate the brittle fracture of elastin, and existing technologies rarely improve the hydration status of the skin through the regulation of water channel proteins.

[0003] Of particular note, while Artemia extract, a novel bioactive ingredient, has demonstrated heat shock protein induction in some studies, no technical solutions have been found for its combination with adenosine or pansy extract. Furthermore, while small peptide fragments unique to hydrolyzed pansy extract have been shown to possess barrier-strengthening properties, they have not been explored for use in elastin protection systems. Existing technologies lack a solution that simultaneously achieves the following goals: precisely combining these ingredients to activate elastin's regenerative energy supply, inhibit enzymatic degradation, repair UV damage, and synergistically regulate the dermal hydration environment.

[0004] Based on this, there is an urgent need in this field to develop a new type of composition that, through a multi-pathway synergistic mechanism, can promote collagen synthesis while establishing a three-in-one protection system of "enzyme activity inhibition-structure reinforcement-damage repair" for elastin, fundamentally breaking through the efficacy bottleneck of existing anti-wrinkle technology. Summary of the Invention

[0005] In view of the shortcomings of the existing technology, the present invention aims to provide a composition with excellent firming and anti-wrinkle effects, in which the components synergize to effectively inhibit elastase synthesis and promote collagen regeneration.

[0006] In order to achieve the above object, the present invention discloses the following technical solutions:

[0007] In a first aspect, the present invention provides a firming and anti-wrinkle composition, comprising the following components in parts by mass:

[0008] Adenosine: 0.5-1.5 parts;

[0009] Sunflower sprout extract: 1-2 parts;

[0010] Hydrolyzed pansy extract: 4-6 parts;

[0011] Artemia extract: 1-2 parts.

[0012] Preferably, the firming and anti-wrinkle composition comprises the following components in parts by mass:

[0013] Adenosine: 0.5-1 part;

[0014] Sunflower sprout extract: 1-1.5 parts;

[0015] Hydrolyzed pansy extract: 5-6 parts;

[0016] Artemia extract: 1.5-2 parts.

[0017] Further preferably, the firming and anti-wrinkle composition comprises the following components in parts by mass:

[0018] Adenosine: 1 part;

[0019] Sunflower sprout extract: 1.5 parts;

[0020] Hydrolyzed pansy extract: 5 parts;

[0021] Artemia extract: 1.5 parts.

[0022] In a second aspect, the present invention provides use of the firming and anti-wrinkle composition described in the first aspect in the preparation of a skin care product with anti-wrinkle and / or firming effects.

[0023] Preferably, the skin care product is in the form of any one of cream, lotion, mask, and essence.

[0024] In a third aspect, the present invention provides a firming and anti-wrinkle essence, comprising the firming and anti-wrinkle composition according to the first aspect, wherein the amount of the firming and anti-wrinkle composition added to the firming and anti-wrinkle essence is 1-3 wt %.

[0025] Preferably, the essence further comprises a moisturizer, a preservative, an emulsifier, and a solvent.

[0026] More preferably, the moisturizing agent is at least one of glycerin, butylene glycol, propylene glycol, 1,2-pentanediol, and dipropylene glycol.

[0027] More preferably, the preservative is phenoxyethanol and / or methylparaben.

[0028] More preferably, the emulsifier is PPG-26-butanediol polyether-26.

[0029] More preferably, the solvent is deionized water.

[0030] In a fourth aspect, the present invention provides a method for preparing the firming and anti-wrinkle essence according to the third aspect, comprising the following steps:

[0031] S1: Heat deionized water with a pH of 5.5-6.5 to 55-65°C, add adenosine, and stir until dissolved to obtain a mixed solution A;

[0032] S2: Cool the mixed solution A to 45-55°C, add a moisturizer, a preservative, and an emulsifier, and stir evenly to obtain a mixed solution B;

[0033] S3: After the mixed solution B is cooled to 35-45°C, the sunflower sprout extract, the hydrolyzed pansy extract and the Artemia extract are added and stirred evenly to obtain a firming and anti-wrinkle essence.

[0034] Beneficial effects of the present invention:

[0035] The present invention innovatively combines adenosine, sunflower sprout extract, hydrolyzed pansy extract, and Artemia extract to produce a firming and anti-wrinkle composition. Studies have shown that adenosine, sunflower sprout extract, hydrolyzed pansy extract, and Artemia extract form a multi-dimensional synergistic mechanism in inhibiting elastase:

[0036] 1. Multi-dimensional synergistic efficiency mechanism

[0037] Through the scientific ratio of adenosine, sunflower sprout extract, hydrolyzed pansy extract and Artemia extract, a three-in-one synergistic network of "energy supply-enzyme activity inhibition-structural reinforcement" is formed: adenosine activates the A2A receptor to promote ATP synthesis in fibroblasts, providing energy for elastin regeneration; Artemia extract (GP4G) achieves long-term protection by inducing heat shock protein 70 to repair DNA damaged by ultraviolet rays; sunflower sprout extract increases NAD+ levels through the SIRT1 pathway, enhances mitochondrial function and inhibits oxidative stress; hydrolyzed pansy extract enhances the skin's hydration barrier by upregulating AQP3 and HAS2; the four act synergistically on multiple signaling pathways such as TGF-β / Smad, Nrf2 / HO-1 and PPARγ, realizing full-link regulation from promoting elastin synthesis to inhibiting degradation.

[0038] 2. Highly effective firming and anti-wrinkle performance

[0039] Experimental data showed that the combination's inhibition rate against elastase reached 47.28% (Example 1), significantly higher than the control group lacking a single component (for example, the inhibition rate was only 29.19% when adenosine was missing). Human testing showed that after 28 days of continuous use, the average depth of fine lines and wrinkles decreased by 15.25%, their volume decreased by 33.80%, and skin elasticity increased by 16.57% (Example 1). These results were significantly superior to those of the control group (p<0.001), demonstrating its excellent firming and anti-wrinkle efficacy.

[0040] 3. Ingredient safety and compatibility advantages

[0041] By optimizing the ratios of the ingredients (e.g., 0.5-1.5 parts adenosine and 4-6 parts hydrolyzed pansy extract), significant effects can be achieved at low dosages (1-3wt%), avoiding the irritation risk associated with high concentrations of a single ingredient. Some ingredients are naturally derived actives (e.g., sunflower sprout and pansy extract), making them gentle and skin-friendly, making them suitable for those with sensitive skin.

[0042] 4. Wide application adaptability

[0043] The composition can be stably formulated into a variety of dosage forms, including creams, lotions, and serums (Examples 1-5). Its preparation process is simple (for example, the serum utilizes a gradient cooling mixing method), making it amenable to industrial production. In particular, in leave-on products (such as serums), it exhibits a long-lasting effect on the dermis, strengthening barrier function. DETAILED DESCRIPTION

[0044] The following will be combined with the embodiments of the present invention to clearly and completely describe the technical solutions of the present invention. Obviously, the embodiments described are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0045] The technical solutions of the present invention are further described in detail below with reference to specific embodiments. It should be understood that the following embodiments are merely used to explain the present invention and are not intended to limit the present invention.

[0046] In the present invention:

[0047] raw material Purchased from Product Name adenosine Guangzhou Baihaobo Co., Ltd. adenosine Sunflower sprout extract Guangzhou Lingyu Trading Co., Ltd. RejuveNAD Hydrolyzed Pansy Extract Guangzhou Bisheng Trading Co., Ltd. AQUAPHYLINE EL Artemia extract Guangzhou Xufan Trading Co., Ltd. GP4G Other raw materials Commercially available -

[0048] Preparation of firming and anti-wrinkle composition

[0049] Each firming and anti-wrinkle composition is prepared by compounding the following components in parts by mass:

[0050] Composition 1: 1 part of adenosine, 1.5 parts of sunflower sprout extract, 5 parts of hydrolyzed pansy extract, and 1.5 parts of Artemia extract.

[0051] Composition 2: 1.5 parts of adenosine, 1 part of sunflower sprout extract, 4 parts of hydrolyzed pansy extract, and 2 parts of Artemia extract.

[0052] Composition 3: 0.5 parts of adenosine, 2 parts of sunflower sprout extract, 6 parts of hydrolyzed pansy extract, and 1 part of Artemia extract.

[0053] Composition ①: Different from composition 1, no adenosine was added, and the missing mass parts were supplemented by sunflower sprout extract, hydrolyzed pansy extract and Artemia extract in a mass ratio of 1.5:5:1.5.

[0054] Composition ②: Different from composition 1, no sunflower sprout extract was added, and the lack of mass parts was made up with adenosine, hydrolyzed pansy extract and Artemia extract in a mass ratio of 1:5:1.5.

[0055] Composition ③: Different from composition 1, no hydrolyzed pansy extract was added, and the missing mass parts were supplemented by adenosine, sunflower sprout extract, and Artemia extract in a mass ratio of 1:1.5:1.5.

[0056] Composition ④: Different from composition 1, no Artemia extract was added, and the missing mass parts were made up with adenosine, sunflower sprout extract and hydrolyzed pansy extract in a mass ratio of 1:1.5:5.

[0057] Composition ⑤: 1 part of adenosine, 5 parts of sunflower sprout extract, 1.5 parts of hydrolyzed pansy extract, and 1.5 parts of Artemia extract.

[0058] Performance test of firming and anti-wrinkle composition:

[0059] Elastase inhibition test

[0060] This test uses porcine pancreatic elastase as the test subject and commercially available N-succinyl-alanine-alanine-alanine-p-nitroanilide (AAAPVN) as the substrate. Porcine pancreatic elastase hydrolyzes AAAPVN, and the hydrolysis product increases absorbance at 420 nm. This absorbance is measured using a microplate reader to evaluate the firming and anti-wrinkle efficacy of the Examples and Comparative Examples.

[0061] 1. Solution Preparation

[0062] (1) Prepare Tris-HCl buffer (0.1 M pH = 7.5): Weigh 2.42 g of Tris into a beaker, add 200 mL of ultrapure water, and adjust the pH to 7.5 with concentrated HCl after complete dissolution.

[0063] (2) Samples to be tested: Take the above compositions respectively, dissolve the compositions with Tris-HCl buffer, and prepare a composition solution with a concentration of 2 wt %, which is set as the sample to be tested.

[0064] (3) Prepare substrate solution AAAPVN (2 mM): Weigh 4.51 mg of N-succinyl-alanine-alanine-alanine-p-nitroaniline and dissolve it in 5 mL of Tris-HCl buffer.

[0065] (4) Prepare porcine pancreatic elastase solution (0.171 U / mL): Dissolve 280 μL of porcine pancreatic elastase stock solution in 10 mL of Tris-HCl buffer.

[0066] 2. Grouping and Sample Addition

[0067] The experiment was divided into three groups: the test sample group, the blank control group, and the model control group. Three replicate wells were set up for each group at the same concentration. The amount of each solution added is shown in Table 1.

[0068] Table 1 Experimental groups

[0069] Sample addition volume (μL) Porcine pancreatic elastase (μL) AAAPVN (μL) Tris-HCl buffer (μL) Model control group 0 25 50 25 Blank control group 0 0 50 50 Sample group to be tested 25 25 50 0

[0070] 3. Determination method

[0071] The reaction was allowed to proceed at room temperature for 15 min, and the absorbance was measured at 420 nm using a microplate reader.

[0072] 4. Calculation formula

[0073] ;

[0074] Where: A0-average absorbance of blank control wells;

[0075] A1-average absorbance of sample wells;

[0076] A2 - average absorbance of model control wells.

[0077] 5. Experimental Results

[0078] Table 2 Results of porcine pancreatic elastase inhibition rate

[0079] Sample name Elastase inhibition rate Model comparison 0 Composition 1 47.28±0.19* Composition 2 47.51±0.14* Composition 3 46.27±0.39* Composition ① 29.19±0.11* Composition ② 37.45±0.26* Composition ③ 33.41±0.06* Composition ④ 35.22±0.14* Composition ⑤ 41.53±0.49*

[0080] Note: “*” indicates p < 0.05 compared with the model control group.

[0081] According to the test results in Table 2, the composition has an inhibitory effect on porcine pancreatic elastase, indicating that the composition has a certain firming and anti-wrinkle effect. The possible synergistic mechanism is as follows:

[0082] Adenosine promotes ATP synthesis in fibroblasts by activating A2A receptors, providing energy for elastin regeneration; Artemia extract (GP4G) repairs UV-damaged DNA by inducing heat shock protein 70, and the two form a complementary mechanism of "instant energy supply + long-term protection"; sunflower sprout extract activates the SIRT1 pathway to increase NAD+ levels, enhance mitochondrial function and scavenge free radicals. Its lupeol component can synergize with Artemia extract to inhibit IR / UV-induced MMP-1 production and block the NF-κB inflammatory pathway to elastin degradation; hydrolyzed pansy increases dermal-epidermal hydration by 50% by upregulating AQP3 water channel protein and HAS2 hyaluronic acid synthase, providing elastic fibers with the moist microenvironment required for resistance to enzymatic degradation, while enhancing the barrier function mediated by CD44 receptors and reducing protease penetration; the four work together to promote elastin synthesis through the TGF-β / Smad pathway, inhibit oxidative stress through the Nrf2 / HO-1 pathway, and regulate the PPARγ pathway to enhance lipid barrier stability.

[0083] In the performance test, compositions lacking any one of adenosine, sunflower sprout extract, hydrolyzed pansy extract, and Artemia extract (i.e., compositions ①-④) were tested. It was found that the inhibitory effects of compositions ①-④ on elastase were all weakened to a certain extent, indicating that each component in the composition is indispensable and that the components are interdependent at a certain ratio.

[0084] In the performance test, a composition with adjusted dosage ratios of the components (i.e., composition ⑤) was tested, and it was found that the inhibitory effect of composition ⑤ on elastase also showed a certain degree of decrease, indicating that the components in the composition have better efficacy under specific ratios.

[0085] Preparation of firming and anti-wrinkle essence

[0086] The components of the firming and anti-wrinkle essence are specifically shown in Table 3. The preparation method of the firming and anti-wrinkle essence comprises the following steps:

[0087] S1: Heat deionized water with a pH of 6 to 60°C, add adenosine, and stir until dissolved to obtain a mixed solution A;

[0088] S2: Cool the mixed solution to 50°C, add a moisturizer, a preservative, and an emulsifier, and stir evenly to obtain a mixed solution B;

[0089] S3: After the mixed solution B is cooled to 40° C., the sunflower sprout extract, the hydrolyzed pansy extract and the Artemia extract are added and stirred evenly to obtain a firming and anti-wrinkle essence.

[0090] The pH of the deionized water can be adjusted by hydrochloric acid or sodium hydroxide.

[0091] Table 3 Components of the firming and anti-wrinkle essence (mass percentage)

[0092]

[0093] Performance test of firming anti-wrinkle serum:

[0094] 1. Test method:

[0095] Fifty volunteers aged 30±3 years with many fine lines and wrinkles, sagging, lack of elasticity, and dry face were screened and randomly divided into 10 groups, with 5 people in each group. Each volunteer used the sample for 4 weeks. The multifunctional 3D skin imaging analyzer Antera3D was used to take photos of the test parts of each group of volunteers before and after using the test product. The elasticity value of the test parts of each group of volunteers before and after using the test product was measured using the elasticity probe of the SKIN-FX skin tester. The data were recorded and expressed as average values to evaluate the anti-wrinkle and firming effects.

[0096] The samples prepared in Examples 1-5 were used as test samples according to Table 4 below.

[0097] Table 4 Usage amount and method

[0098] Use area face Usage About 3.0g (3mL). Frequency of use and usage cycle Use once in the morning and evening for 28 consecutive days How to use After cleansing, apply the sample to the entire face and massage until completely absorbed

[0099] Before the test, volunteers carefully read and signed the informed consent form. The samples were confirmed to be mild to the skin through human patch tests and no adverse reactions occurred. The experiment used a before-and-after comparison method with a test period of 4 weeks and skin data collection times of 3 times, at 0 days and 28 days respectively.

[0100] 2. Evaluation basis:

[0101] Positive determination:

[0102] Comparing the samples before and after use, the average depth and volume of fine lines / wrinkles / folds were significantly improved (p < 0.05), indicating that the samples have anti-wrinkle effects.

[0103] Comparing the samples before and after use, the skin elasticity value was significantly improved (p < 0.05), indicating that the samples have a firming effect.

[0104] Negative determination:

[0105] Comparing the samples before and after use, there was no significant improvement in the average depth and volume of fine lines / wrinkles / folds (p≥0.05), indicating that the samples had no anti-wrinkle effect.

[0106] Comparing the samples before and after use, there was no significant improvement in skin elasticity (p≥0.05), indicating that the samples had no firming effect.

[0107] Irritation evaluation: Observe the skin condition of the volunteers 30 minutes after using the sample. If the result is negative, continue to observe for 24 hours.

[0108] Judging criteria:

[0109] Grade 0: negative reaction;

[0110] Grade 1: Suspicious reaction, only slight erythema;

[0111] Grade 2: weak positive reaction, erythema, infiltration, edema, and papules may be present;

[0112] Grade 3: Strong positive reaction, with erythema, infiltration, edema, papules, and the reaction may extend beyond the test area;

[0113] Grade 4: Very strong positive reaction, obvious erythema, severe infiltration, edema, confluent blister, and reaction beyond the test area.

[0114] 3. Calculation formula

[0115] Change rate%=(X 28d -X 0d ) / X 0d ×100%

[0116] Where X is the measured value of each skin index; 28d is the measured value on the 28th day, and 0d is the measured value on the 0th day; the specific results are shown in Table 5.

[0117] Table 5 Test results

[0118] experimental group Average depth change of fine lines / wrinkles / folds / % Average volume change rate of fine lines / wrinkles / folds / % Fine lines / wrinkles / folds elasticity value change rate / % Irritation (level) Example 1 <![CDATA[-15.25 *** ]]> <![CDATA[-33.80 *** ]]> <![CDATA[16.57 *** ]]> 0 Example 2 <![CDATA[-14.51 *** ]]> <![CDATA[-30.47 *** ]]> <![CDATA[15.42 *** ]]> 0 Example 3 <![CDATA[-15.55 *** ]]> <![CDATA[-34.26 *** ]]> <![CDATA[16.93 *** ]]> 0 Example 4 <![CDATA[-15.27 *** ]]> <![CDATA[-33.91 *** ]]> <![CDATA[16.62 *** ]]> 0 Example 5 <![CDATA[-15.04 *** ]]> <![CDATA[-33.25 *** ]]> <![CDATA[16.51 *** ]]> 0 Comparative Example ① <![CDATA[-7.63 *** ]]> <![CDATA[-20.26 *** ]]> <![CDATA[9.35 *** ]]> 0 Comparative Example ② <![CDATA[-11.52 *** ]]> <![CDATA[-24.35 *** ]]> <![CDATA[12.52 *** ]]> 0 Comparative Example ③ <![CDATA[-10.18 *** ]]> <![CDATA[-22.07 *** ]]> <![CDATA[11.16 *** ]]> 0 Comparative Example ④ <![CDATA[-11.07 *** ]]> <![CDATA[-23.52 *** ]]> <![CDATA[11.94 *** ]]> 0 Comparative Example ⑤ <![CDATA[-13.62 *** ]]> <![CDATA[-29.79 *** ]]> <![CDATA[14.86 *** ]]> 0

[0119] Note: “***” indicates comparison with before using the product, p < 0.001.

[0120] Result analysis:

[0121] According to the test results in Table 5, the essence provided by the present invention has a firming and anti-wrinkle effect. Compared with the results of Examples 1-3, it can be seen that the firming and anti-wrinkle performance effect has a certain dependence on the concentration of the composition.

[0122] Comparing the results of Examples 1, 4-5, it can be seen that the dosage ratio of the various raw materials in the firming and anti-wrinkle composition has a certain influence on the firming and anti-wrinkle effect.

[0123] Comparison of the results of Example 1 with Comparative Examples ①-⑤ shows that the raw materials in the firming and anti-wrinkle composition have a synergistic effect.

[0124] Furthermore, according to the results of the irritation test, the essence provided by the present invention is safe and non-irritating.

[0125] Finally, it should be noted that the above descriptions are merely preferred embodiments of the present invention and are not intended to limit the present invention. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art will be able to modify the technical solutions described in the aforementioned embodiments or substitute equivalents for some of the technical features. Any modifications, equivalent substitutions, and improvements made within the spirit and principles of the present invention shall be included within the scope of protection of the present invention.

Claims

1. A firming and anti-wrinkle composition, characterized in that: The composition consists of the following components in parts by mass: Adenosine: 0.5-1.5 parts; Sunflower sprout extract: 1-2 parts; Hydrolyzed pansy extract: 4-6 parts; Artemia extract: 1-2 parts.

2. The composition according to claim 1, characterized in that The composition consists of the following components in parts by mass: Adenosine: 0.5-1 part; Sunflower sprout extract: 1-1.5 parts; Hydrolyzed pansy extract: 5-6 parts; Artemia extract: 1.5-2 parts.

3. The composition according to claim 1, characterized in that The composition consists of the following components in parts by mass: Adenosine: 1 part; Sunflower sprout extract: 1.5 parts; Hydrolyzed pansy extract: 5 parts; Artemia extract: 1.5 parts.

4. Use of the composition according to any one of claims 1 to 3 in the preparation of a skin care product with anti-wrinkle and / or firming effects.

5. The application according to claim 4, characterized in that: The skin care product is in the form of any one of cream and lotion.

6. The application according to claim 4, characterized in that: The skin care product dosage form is any one of essence and facial mask.

7. A firming and anti-wrinkle essence, characterized in that: The invention comprises 1-3 wt % of the firming and anti-wrinkle composition according to any one of claims 1 to 3.

8. The essence according to claim 7, characterized in that The composition further comprises 1-3 wt% of a moisturizing agent, 0.5-1.5 wt% of a preservative, 0.5-1.5 wt% of an emulsifier, and deionized water to make up to 100 wt%.

9. The essence according to claim 8, characterized in that The moisturizing agent is one or more of glycerin, butylene glycol, propylene glycol, 1,2-pentanediol, and dipropylene glycol; the preservative is phenoxyethanol and / or methylparaben.

10. The essence according to claim 8, characterized in that The emulsifier is PPG-26-butanediol polyether-26.

11. A method for preparing the firming and anti-wrinkle essence according to any one of claims 8 to 10, characterized in that: The following steps are involved: S1: Heat deionized water with a pH of 5.5-6.5 to 55-65°C, add adenosine, and stir until dissolved to obtain a mixed solution A; S2: Cool the mixed solution A to 45-55°C, add a moisturizer, a preservative, and an emulsifier, and stir evenly to obtain a mixed solution B; S3: After the mixed solution B is cooled to 35-45°C, the sunflower sprout extract, the hydrolyzed pansy extract and the Artemia extract are added and stirred evenly to obtain a firming and anti-wrinkle essence.

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