Bacteriostatic nursing cream, cream paper and preparation method thereof

Abstract

The invention relates to the field of creams, in particular to a bacteriostatic nursing cream, cream paper and a preparation method thereof. The bacteriostatic nursing cream comprises the following substances in parts by weight: 3-8 parts of bacteriostatic polysaccharide; 70 to 75 parts of glycerol; 1 to 3 parts of propylene glycol; 8-12 parts of a softening agent; 0.01 to 0.05 part of a surfactant; and the antibacterial polysaccharide is beta-glucan. The components of the bacteriostatic nursing cream are optimized, beta-glucan is selected as a main bacteriostatic agent, meanwhile, high-concentration glycerol is selected to form a hydrogen bond dominated three-dimensional gel network in a system, and the three-dimensional gel network is used as a moisturizer to maintain a skin barrier and is also used as a slow-release carrier to wrap beta-glucan, so that the burst release risk of beta-glucan is reduced. Through proportional threshold design, the components form dynamic balance in physicochemical properties (viscosity, charge and hydrogen-bond interaction), and bacteriostasis, slow release and use experience are considered.

Description

Technical Field

[0001] The present application relates to the field of creams, and in particular to an antibacterial care cream, cream paper and a preparation method thereof. Background Art

[0002] As an important branch of the health field, female intimate care is related to physiological health and quality of life. Due to the unique anatomical structure of the female reproductive system, its mucosal barrier is susceptible to invasion by pathogenic microorganisms, leading to a high incidence of bacterial vaginitis, candidal infection and other diseases.

[0003] Traditional private care products mostly rely on antibiotics (such as metronidazole, clindamycin) or broad-spectrum chemical antibacterial agents (such as triclosan). Although they can quickly inhibit pathogenic bacteria, long-term use can easily destroy the balance of vaginal microecology, induce the emergence of drug-resistant strains, and even increase the risk of cervical lesions. Therefore, the development of new care products that have both efficient antibacterial, microecological friendly and long-term sustained-release properties has become a technical bottleneck that needs to be broken through in this field.

[0004] In view of the above-mentioned prior art, the inventors found that there is a contradiction between the single antibacterial ingredients of existing private care products and the destruction of the microecology. Although quaternary ammonium salt chemical antibacterial agents can quickly kill pathogens, the indiscriminate inhibition leads to a decrease in the survival rate of lactobacilli, exacerbating the risk of imbalance in the flora; secondly, the release control of the dosage form is insufficient. Traditional creams rely on simple physical mixing, and the release rate of active ingredients exceeds 90% within 24 hours after contact with body fluids, and it is impossible to maintain long-term antibacterial effects. Finally, due to the poor adaptability of traditional creams and other materials and processes. The existing coating process causes the paper base to absorb liquid too high, which not only affects the touch of use, but also causes the aggregation and inactivation of β-glucan during the drying process. Summary of the invention

[0005] In order to improve the above technical problems, the present application provides an antibacterial care cream, cream paper and a preparation method thereof.

[0006] In the first aspect, the present application provides an antibacterial care cream, which adopts the following technical solution:

[0007] An antibacterial care cream comprises the following materials in parts by weight: 3-8 parts of antibacterial polysaccharide; 70-75 parts of glycerol; 1-3 parts of propylene glycol; 8-12 parts of softener; and 0.01-0.05 parts of surfactant. The antibacterial polysaccharide is beta-glucan.

[0008] Through the above technical solution, the present application optimizes the components of the antibacterial nursing cream. 3-8 parts by weight of β-glucan is selected as the main antibacterial agent. On the one hand, as the core antibacterial component, if the concentration of β-glucan is too low, an effective antibacterial network cannot be formed; while if the concentration is too high, the viscosity of the cream will be too high, affecting the coating uniformity. On the other hand, the β-glycosidic bond structure of the β-glucan selected in the present application can specifically bind to the bacterial surface receptor, activate the immune response and destroy the cell wall of pathogenic bacteria.

[0009] Meanwhile, the present application selects high-concentration glycerol to form a three-dimensional gel network dominated by hydrogen bonds in the system, which not only acts as a moisturizer to maintain the skin barrier, but also acts as a slow-release carrier to encapsulate β-glucan, reducing its burst release risk. As a co-solvent, it synergistically reduces the system viscosity with glycerol, and at the same time regulates the dissolved state and self-assembly behavior of β-glucan. The softener fills the pores of the gel network, reduces the surface friction coefficient, and improves the use comfort. The surfactant binds to β-glucan (negatively charged) through electrostatic interaction to form a "polysaccharide-antibacterial agent" complex, enhancing the penetration of the outer membrane of Gram-negative bacteria.

[0010] Therefore, through the design of proportional thresholds, the present application enables the components to form a dynamic balance in physical and chemical properties (viscosity, charge, hydrogen bond interaction), taking into account antibacterial, slow-release and user experience.

[0011] Further, the β-glucan includes at least one of β-1,3-glucan, β-1,4-glucan or β-1,6-glucan.

[0012] Through the above technical solution, the present application further optimizes the type of β-glucan. The main chain β-1,3 bond of β-1,3-glucan forms a helical structure, and the side chain β-1,6 bond increases the steric hindrance, making it easier to bind to the Dectin-1 receptor on the surface of macrophages and activate the secretion of cytokines such as IL-1β (immune regulation antibacterial). Its helical structure has the best stability in the glycerol gel. The linear structure of β-1,4-glucan makes it easier to penetrate the bacterial biofilm and inhibits biofilm formation by blocking the polysaccharide matrix synthase. The highly branched structure of β-1,6-glucan can physically block bacterial adhesion. By selectively combining different glycosidic bond types, multi-target antibacterial (immune activation, biofilm inhibition, physical barrier) is achieved, while avoiding the functional limitations of a single structure.

[0013] Further, the molecular weight of the β-glucan is 50-200 kDa.

[0014] Through the above technical solution, the molecular weight of β-glucan in the present application is optimized. Since β-glucan with a molecular weight lower than 50 kDa has insufficient chain length and cannot effectively crosslink to form a nanofiber network, the sustained-release performance decreases. In addition, small-molecule β-glucan easily penetrates the vaginal mucosa and enters the bloodstream, increasing the risk of systemic immune activation. When the molecular weight is higher than 200 kDa, the solubility of β-glucan in the glycerol-propylene glycol system decreases sharply, and high-shear stirring easily causes molecular chain breakage, generating free radical by-products. Therefore, the present application selects β-glucan with a molecular weight of 50-200 kDa, taking into account antibacterial activity, sustained-release performance, and process feasibility.

[0015] Further, the softener includes at least one of polydimethylsiloxane or fatty acid ester compounds.

[0016] Through the above technical solution, the present application defines the softener as polydimethylsiloxane or fatty acid ester compounds. Among them, the low surface tension of polydimethylsiloxane can reduce the contact angle between the cream and paper fibers, promoting the uniform penetration of the cream. The viscoelasticity of polydimethylsiloxane can also buffer the stress concentration during the deformation of the paper base. As a polar softener, the ester group of fatty acid ester compounds forms hydrogen bonds with glycerol, enhancing the denseness of the gel network. At the same time, the hydrophobic end of the ester chain covers the hydroxyl groups of paper fibers, reducing water absorption.

[0017] Further, the surfactant includes at least one of cetylpyridinium chloride, ethyl lauroyl arginate, or benzalkonium chloride.

[0018] Through the above technical solution, the present application selects a specific type of surfactant to achieve physical-chemical and biological synergistic antibacterial effects, and at the same time optimizes the loading and release behavior of active ingredients by using intermolecular forces.

[0019] In a second aspect, the present application provides an antibacterial care cream paper, adopting the following technical solution:

[0020] An antibacterial care cream paper includes the antibacterial care cream described in any one of the above.

[0021] Through the above technical solution, the present application loads the cream onto the surface of the paper-based material. Since the fiber pores of the paper-based material and the β-glucan nanofibers form an interpenetrating network, physically intercepting pathogenic bacteria (interception efficiency > 95%), while allowing Lactobacillus to pass freely, achieving selective antibacterial effects. Secondly, the viscoelasticity of the cream ensures that it forms a continuous film on the paper surface rather than penetrating the inner layer, avoiding the inactivation of active ingredients by fiber adsorption. Finally, through the paper base as a mechanical support layer and the cream as an active layer, a "physical interception + chemical and biological antibacterial" double barrier is formed, breaking through the limitations of the single-action mode of traditional antibacterial paper.

[0022] Thirdly, the present application provides a preparation method of an antibacterial nursing cream paper, adopting the following technical solutions:

[0023] A preparation method of an antibacterial nursing cream paper includes the following preparation steps:

[0024] Take glycerol and propylene glycol and place them in a reaction kettle, stir at low temperature and low speed, and collect the liquid phase;

[0025] Add antibacterial polysaccharide in portions and increase the temperature and speed for stirring to induce the self-assembly of antibacterial polysaccharide to form nanofiber gel liquid;

[0026] Take a surfactant and spray it into the nanofiber gel liquid, then add a softener, lower the temperature and stir, and collect the liquid antibacterial nursing cream;

[0027] Take the antibacterial nursing cream and coat it on the surface of the virgin wood pulp paper until the moisture content reaches 25%, and then the antibacterial nursing cream paper can be prepared.

[0028] Through the above technical solutions, the present application first suppresses the thermal motion of glycerol molecules by low-temperature and low-speed premixing to make them orderly arranged to form a pre-gel network; low-speed stirring avoids introducing air bubbles and ensures the homogeneity of the liquid phase; secondly, the present application adds β-glucan in portions and increases the temperature and speed. Adding in portions avoids excessive local concentration leading to aggregation; increasing the temperature reduces the viscosity of the system, promotes the movement of β-glucan chain segments, and arranges them directionally into nanofibers under shear force. The micro-vortex generated by increasing the speed of stirring enhances the uniformity of fiber dispersion; at the same time, the present application injects the surfactant by spraying, and it is adsorbed on the surface of β-glucan fibers through liquid-liquid interface self-assembly, reducing the damage of free surfactant to the gel network. Finally, the present application lowers the temperature to fix the gel network, and adding a softener and then stirring at low speed avoids shear thinning and maintains the integrity of the network. This method realizes the goal of efficient loading of active ingredients and structural stability through precise control of temperature-shear force-loading sequence.

[0029] Further, the low temperature and low speed means stirring at a low speed of 200 rpm for 10 min at 40°C.

[0030] Further, the coating amount of the antibacterial nursing cream is 10 - 20 g / m 2 .

[0031] Through the above technical solutions, the present application optimizes the coating amount, makes the thickness of the cream layer moderate, the β-glucan loading amount meets the antibacterial requirement for 8 hours, and at the same time the mechanical properties of the paper base reach the best effect.

[0032] In summary, the present application has the following beneficial effects:

[0033] First, this application achieves a multi-dimensional precision attack on pathogens through the molecular synergistic design of β-glucan and surfactant. β-glucan, with its unique spatial conformation, can specifically recognize and bind to bacterial surface receptors, and significantly improve the antibacterial efficiency by destroying the integrity of the cell wall, interfering with metabolic pathways, and activating the triple mechanism of local immune response. At the same time, the surfactant is embedded in the β-glucan network through electrostatic adsorption or hydrophobic action to form a dynamic sustained-release reservoir, which gradually releases the active ingredients after contacting body fluids, avoiding the short-term failure problem of traditional products caused by the sudden release of ingredients. This "rapid onset-long-term maintenance" synergistic mode not only effectively inhibits common pathogens such as Escherichia coli, Staphylococcus aureus, and Candida albicans, but also blocks the formation and regeneration of bacterial biofilms, significantly reducing the recurrence rate of gynecological infections.

[0034] Second, this application breaks through the technical bottleneck of poor compatibility between paper-based carriers and active ingredients through material-process collaborative design. The synergistic effect of the softener and gel network in the cream enables the paper base to maintain excellent flexibility and skin comfort after loading high-concentration active ingredients, avoiding the hardening and brittle cracking problems of traditional antibacterial paper. The "dense outer layer-porous inner layer" gradient structure constructed by the segmented drying process not only ensures the firm combination of the cream layer and the paper fiber, but also realizes the on-demand release of active ingredients through pore regulation. In addition, the formation of self-assembled nanofibers enhances the mechanical stability of the cream, allowing it to withstand changes in temperature and humidity during storage, transportation and use, and avoid inactivation of ingredients or stratification failure. This structural-functional integrated design enables the product to have both high-efficiency antibacterial properties, ease of use and long-term stability, meeting the diversified needs of daily care and special scenarios.

[0035] Third, this application is different from the broad-spectrum lethality of traditional chemical antibacterial agents. The cream paper achieves the dual goals of selective inhibition of pathogens and protection of probiotic activity by optimizing the proportion of ingredients and regulating release dynamics. While destroying the structure of pathogenic bacteria, β-glucan can provide the carbon source required for the proliferation of vaginal lactobacilli, promote their metabolic acid production, and maintain the acidic environment of the vagina. Surfactants only play a role in the membrane structure of pathogens at low concentrations, and have little effect on the stability of lactobacilli membranes. In addition, the hydrophilic gel layer formed by the high content of glycerol in the cream can simulate the natural mucus environment and reduce the physical stimulation of the mucosal barrier. This "harm-inhibiting and benefit-protecting" characteristic can not only quickly relieve inflammatory symptoms, but also maintain the homeostasis of vaginal flora for a long time, avoiding the risk of secondary infection caused by microecological imbalance in traditional products. DETAILED DESCRIPTION

[0036] The present application is further described in detail below with reference to the embodiments.

[0037] Example

[0038] Example 1

[0039] An antibacterial nursing cream comprises the following substances by weight: 3 kg of β-1,3-glucan with a molecular weight of 50 kDa, 70 kg of glycerol, 1 kg of propylene glycol, 8 kg of softener polydimethylsiloxane, and 0.01 kg of surfactant cetylpyridinium chloride;

[0040] A preparation method of an antibacterial nursing cream paper comprises the following steps:

[0041] Take glycerol and propylene glycol and place them in a reaction kettle. At 40 °C, stir at a low speed of 200 rpm for 10 min, and collect the liquid phase;

[0042] Add the antibacterial polysaccharide in three equal masses and raise the temperature to 50 °C, and stir at a stirring speed of 400 rpm for 25 min to induce the self-assembly of the antibacterial polysaccharide to form a nanofiber gel liquid;

[0043] Take the surfactant and spray it into the nanofiber gel liquid, then add the softener, and stir at 25 °C for 20 min to collect the liquid antibacterial nursing cream;

[0044] Take the liquid antibacterial nursing cream at 10 g / m 2 Coat it on the surface of the virgin wood pulp paper with a moisture content of 25%, and the antibacterial nursing cream paper can be prepared.

[0045] Example 2

[0046] An antibacterial nursing cream comprises the following substances by weight: 5 kg of β-1,3-glucan with a molecular weight of 50 kDa, 72 kg of glycerol, 2 kg of propylene glycol, 10 kg of softener polydimethylsiloxane, and 0.03 kg of surfactant cetylpyridinium chloride;

[0047] A preparation method of an antibacterial nursing cream paper comprises the following steps:

[0048] Take glycerol and propylene glycol and place them in a reaction kettle. At 40 °C, stir at a low speed of 200 rpm for 10 min, and collect the liquid phase;

[0049] Add the antibacterial polysaccharide in three equal masses and raise the temperature to 50 °C, and stir at a stirring speed of 400 rpm for 25 min to induce the self-assembly of the antibacterial polysaccharide to form a nanofiber gel liquid;

[0050] Take the surfactant and spray it into the nanofiber gel liquid, then add the softener, and stir at 25 °C for 20 min to collect the liquid antibacterial nursing cream;

[0051] Take the liquid antibacterial nursing cream at 10 g / m 2 Coat it on the surface of the virgin wood pulp paper until the moisture content is 25%, and the antibacterial nursing cream paper can be prepared.

[0052] Example 3

[0053] An antibacterial nursing cream comprises the following substances by weight: 8 kg of β-1,3-glucan with a molecular weight of 50 kDa, 75 kg of glycerol, 3 kg of propylene glycol, 12 kg of softener polydimethylsiloxane, and 0.05 kg of surfactant cetylpyridinium chloride;

[0054] A preparation method of an antibacterial nursing cream paper comprises the following steps:

[0055] Take glycerol and propylene glycol and place them in a reaction kettle. At 40 °C, stir at a low speed of 200 rpm for 10 min, and collect the liquid phase;

[0056] Add the antibacterial polysaccharide in three equal masses and raise the temperature to 50 °C. Stir at a stirring speed of 400 rpm for 25 min to induce the self-assembly of the antibacterial polysaccharide to form a nanofiber gel liquid;

[0057] Take the surfactant and spray it into the nanofiber gel liquid, then add the softener, and stir at 25 °C for 20 min to collect the liquid antibacterial nursing cream;

[0058] Take the liquid antibacterial nursing cream at 10 g / m 2 Coat it on the surface of the virgin wood pulp paper until the moisture content reaches 25%, and the antibacterial nursing cream paper can be prepared.

[0059] Example 4

[0060] An antibacterial nursing cream comprises the following substances by weight: 5 kg of β-1,3-glucan with a molecular weight of 200 kDa, 72 kg of glycerol, 2 kg of propylene glycol, 10 kg of softener polydimethylsiloxane, and 0.03 kg of surfactant cetylpyridinium chloride;

[0061] A preparation method of an antibacterial nursing cream paper comprises the following steps:

[0062] Take glycerol and propylene glycol and place them in a reaction kettle. At 40 °C, stir at a low speed of 200 rpm for 10 min, and collect the liquid phase;

[0063] Add the antibacterial polysaccharide in three equal masses and raise the temperature to 50 °C. Stir at a stirring speed of 400 rpm for 25 min to induce the self-assembly of the antibacterial polysaccharide to form a nanofiber gel liquid;

[0064] Take the surfactant and spray it into the nanofiber gel liquid, then add the softener, and stir at 25 °C for 20 min to collect the liquid antibacterial nursing cream;

[0065] Take the liquid antibacterial nursing cream at 10 g / m 2Coat the surface of the virgin wood pulp paper until the moisture content reaches 25%, and the antibacterial care cream paper can be prepared.

[0066] Example 5

[0067] An antibacterial care cream contains the following substances by weight: 5 kg of β-1,4-glucan with a molecular weight of 50 kDa, 72 kg of glycerol, 2 kg of propylene glycol, 10 kg of softener polydimethylsiloxane, and 0.03 kg of surfactant cetylpyridinium chloride;

[0068] A preparation method of an antibacterial care cream paper includes the following steps:

[0069] Put glycerol and propylene glycol into a reaction kettle, stir at a low speed of 200 rpm at 40 °C for 10 min, and collect the liquid phase;

[0070] Add the antibacterial polysaccharide in three equal masses and raise the temperature to 50 °C, stir at a stirring speed of 400 rpm for 25 min to induce the self-assembly of the antibacterial polysaccharide to form a nanofiber gel liquid;

[0071] Take the surfactant and spray it into the nanofiber gel liquid, then add the softener, and stir at 25 °C for 20 min to collect the liquid antibacterial care cream;

[0072] Take the liquid antibacterial care cream at 10 g / m 2 Coat the surface of the virgin wood pulp paper until the moisture content reaches 25%, and the antibacterial care cream paper can be prepared.

[0073] Example 6

[0074] An antibacterial care cream contains the following substances by weight: 5 kg of β-1,6-glucan with a molecular weight of 50 kDa, 72 kg of glycerol, 2 kg of propylene glycol, 10 kg of softener polydimethylsiloxane, and 0.03 kg of surfactant cetylpyridinium chloride;

[0075] A preparation method of an antibacterial care cream paper includes the following steps:

[0076] Put glycerol and propylene glycol into a reaction kettle, stir at a low speed of 200 rpm at 40 °C for 10 min, and collect the liquid phase;

[0077] Add the antibacterial polysaccharide in three equal masses and raise the temperature to 50 °C, stir at a stirring speed of 400 rpm for 25 min to induce the self-assembly of the antibacterial polysaccharide to form a nanofiber gel liquid;

[0078] Take the surfactant and spray it into the nanofiber gel liquid, then add the softener, and stir at 25 °C for 20 min to collect the liquid antibacterial care cream;

[0079] Take the liquid antibacterial nursing cream and apply it at 10 g / m 2 onto the surface of the virgin wood pulp paper until the moisture content reaches 25%, then the antibacterial nursing cream paper can be prepared.

[0080] Example 7

[0081] An antibacterial nursing cream comprises the following substances by weight: 5 kg of β-1,6-glucan with a molecular weight of 50 kDa, 72 kg of glycerol, 2 kg of propylene glycol, 10 kg of the softener isopropyl myristate, and 0.03 kg of the surfactant cetylpyridinium chloride;

[0082] A method for preparing an antibacterial nursing cream paper comprises the following steps:

[0083] Take glycerol and propylene glycol and place them in a reaction kettle. At 40 °C, stir at a low speed of 200 rpm for 10 min, and collect the liquid phase;

[0084] Add the antibacterial polysaccharide in three equal masses and raise the temperature to 50 °C, and stir at a stirring speed of 400 rpm for 25 min to induce the self-assembly of the antibacterial polysaccharide to form a nanofiber gel liquid;

[0085] Take the surfactant and spray it into the nanofiber gel liquid, then add the softener, and stir at 25 °C for 20 min, and collect the liquid antibacterial nursing cream;

[0086] Take the liquid antibacterial nursing cream and apply it at 10 g / m 2 onto the surface of the virgin wood pulp paper until the moisture content reaches 25%, then the antibacterial nursing cream paper can be prepared.

[0087] Example 8

[0088] An antibacterial nursing cream comprises the following substances by weight: 5 kg of β-1,6-glucan with a molecular weight of 50 kDa, 72 kg of glycerol, 2 kg of propylene glycol, 10 kg of the softener isopropyl myristate, and 0.03 kg of the surfactant ethyl lauroyl arginate;

[0089] A method for preparing an antibacterial nursing cream paper comprises the following steps:

[0090] Take glycerol and propylene glycol and place them in a reaction kettle. At 40 °C, stir at a low speed of 200 rpm for 10 min, and collect the liquid phase;

[0091] Add the antibacterial polysaccharide in three equal masses and raise the temperature to 50 °C, and stir at a stirring speed of 400 rpm for 25 min to induce the self-assembly of the antibacterial polysaccharide to form a nanofiber gel liquid;

[0092] Take a surfactant and spray it into the nanofiber gel solution, then add a softener, and stir at 25°C for 20 min to collect a liquid antibacterial nursing cream;

[0093] Take the liquid antibacterial nursing cream at 10 g / m 2 Coat it on the surface of the virgin wood pulp paper until the moisture content reaches 25%, and then the antibacterial nursing cream paper can be prepared.

[0094] Example 9

[0095] An antibacterial nursing cream includes the following substances by weight: 5 kg of β-1,6-glucan with a molecular weight of 50 kDa, 72 kg of glycerol, 2 kg of propylene glycol, 10 kg of softener isopropyl myristate, and 0.03 kg of surfactant benzalkonium chloride;

[0096] A preparation method of an antibacterial nursing cream paper includes the following steps:

[0097] Put glycerol and propylene glycol into a reaction kettle, and stir at a low speed of 200 rpm at 40°C for 10 min to collect a liquid phase;

[0098] Add antibacterial polysaccharide in three equal masses and raise the temperature to 50°C, and stir at a stirring speed of 400 rpm for 25 min to induce the self-assembly of antibacterial polysaccharide to form a nanofiber gel solution;

[0099] Take a surfactant and spray it into the nanofiber gel solution, then add a softener, and stir at 25°C for 20 min to collect a liquid antibacterial nursing cream;

[0100] Take the liquid antibacterial nursing cream at 10 g / m 2 Coat it on the surface of the virgin wood pulp paper until the moisture content reaches 25%, and then the antibacterial nursing cream paper can be prepared.

[0101] Example 10

[0102] An antibacterial nursing cream includes the following substances by weight: 5 kg of β-1,6-glucan with a molecular weight of 50 kDa, 72 kg of glycerol, 2 kg of propylene glycol, 10 kg of softener isopropyl myristate, and 0.03 kg of surfactant benzalkonium chloride;

[0103] A preparation method of an antibacterial nursing cream paper includes the following steps:

[0104] Put glycerol and propylene glycol into a reaction kettle, and stir at a low speed of 200 rpm at 40°C for 10 min to collect a liquid phase;

[0105] Add the antibacterial polysaccharide in three equal masses and heat up to 50 °C, stir for 25 min at a stirring speed of 400 rpm to induce the self-assembly of the antibacterial polysaccharide to form a nanofiber gel liquid;

[0106] Take the surfactant and spray it into the nanofiber gel liquid, then add the softener, stir at 25 °C for 20 min, and collect the liquid antibacterial nursing cream;

[0107] Take the liquid antibacterial nursing cream at 20 g / m 2 Coat it on the surface of the virgin wood pulp paper until the moisture content reaches 25%, and the antibacterial nursing cream paper can be prepared.

[0108] Comparative Example 1

[0109] An antibacterial nursing cream, compared with Example 1, uses chitosan (deacetylation degree ≥ 85%, molecular weight 150 kDa) of equal mass to replace β-1,3-glucan, and the other components and steps are the same as those in Example 1.

[0110] Comparative Example 2

[0111] An antibacterial nursing cream, compared with Example 1, stir and mix glycerol, propylene glycol, antibacterial polysaccharide, surfactant and softener together to prepare the cream; take the cream at 10 g / m 2 Coat it on the surface of the virgin wood pulp paper and dry it at 25 °C for 12 h until the moisture content reaches 25% to prepare the cream paper.

[0112] Performance testing

[0113] 1. Antibacterial rate test standard: GB / T 20944.3-2008 "Evaluation of antibacterial properties of textiles - Part 3: Oscillation method";

[0114] Test method:

[0115] (1) Bacterial species selection: Escherichia coli (ATCC 25922), Candida albicans (ATCC 10231).

[0116] (2) Operating steps:

[0117] Cut the cream paper into 5×5 cm 2 specimens, soak them in 10 mL PBS buffer (pH 7.4), and ultrasonically extract for 30 min; take 100 μL of the extract and mix it with 900 μL of the bacterial suspension (1×10 6 CFU / mL), incubate at 37 °C with shaking for 24 h; dilute and spread on an agar plate, incubate at 37 °C for 24 h, and count the number of colonies.

[0118] 2. Lactobacillus survival rate test

[0119] Test standard: ISO 16140-3:2021 "Food chain microbiology - Method validation - Part 3: Strain-specific validation protocol"

[0120] Test method: Strain: Lactobacillus (ATCC 33820). Operating steps:

[0121] Mix the extract of the cream paper with the Lactobacillus suspension (1×10 6 CFU / mL) in equal volume, and incubate anaerobically at 37°C for 24 h; detect the viable bacteria ratio by flow cytometry (PI / FDA double staining).

[0122] 3. Sustained-release performance test

[0123] Test standard: USP<711> "Dissolution test" (for drug release)

[0124] Test method: Simulated release medium: Artificial vaginal fluid (pH 4.5, containing 0.5% NaCl, 0.1% glucose).

[0125] Operating steps: Place the cream paper in 100 mL of the release medium, and oscillate at a constant temperature of 37°C (100 rpm). Take 5 mL of samples at preset time points (2 h, 6 h, 24 h, 48 h), and supplement with an equal amount of fresh medium. Detect the concentrations of β-glucan and surfactant by HPLC (chromatographic conditions: C18 column, mobile phase acetonitrile - water, detection wavelength 210 nm).

[0126] The detection results are shown in Table 1 below:

[0127] Table 1 Performance detection table

[0128]

[0129]

[0130]

[0131] It can be found from the comparison between Examples 1-9 and Comparative Example 1 that the technical solution of the present application has good antibacterial effects against Escherichia coli and Candida albicans. By comparing the antibacterial polysaccharides used in Examples 1-3 and Comparative Example 1, it shows that through the molecular synergistic design of β-glucan and surfactant, the present application realizes multi-dimensional precise strikes against pathogenic bacteria. It can effectively inhibit common pathogenic bacteria such as Escherichia coli, Staphylococcus aureus, and Candida albicans, and can also block the formation and regeneration of bacterial biofilms, significantly reducing the recurrence rate of gynecological infections.

[0132] Further comparing with Examples 1-9 and Comparative Example 2, it shows that the technical solution of the present application first suppresses the thermal motion of glycerol molecules through low-temperature and low-speed premixing to make them arrange orderly to form a pre-gel network; low-speed stirring avoids introducing air bubbles and ensures the homogeneity of the liquid phase; secondly, the present application adds β-glucan in batches and increases the temperature and speed. Adding in batches avoids excessive local concentration leading to aggregation; increasing the temperature reduces the viscosity of the system, promotes the movement of β-glucan segments, and arranges them into nanofibers under shear force. The micro-vortices generated by increasing the stirring speed enhance the uniformity of fiber dispersion; at the same time, the present application injects surfactant by spraying, and self-assembles and adsorbs on the surface of β-glucan fibers through the liquid-liquid interface, reducing the damage of free surfactant to the gel network. Finally, the present application cools down to fix the gel network, and low-speed stirring after adding the softener avoids shear thinning and maintains the integrity of the network. This method realizes the efficient loading of active ingredients and structural stability through the precise control of temperature-shear force-feeding sequence.

[0133] Finally, looking at the technical solutions of Examples 1-9, the technical solution of Example 10 prepares the best cream paper, achieving triple optimization of antibacterial-microecology-flexibility.

[0134] This specific embodiment is only an explanation of the present application, and it is not a limitation of the present application. Those skilled in the art can make modifications without creative contributions to this embodiment according to needs after reading this specification, but as long as it is within the scope of the claims of the present application, it is protected by the patent law.

Claims

1. An antibacterial nursing cream, characterized in that, Comprising the following substances in parts by weight: The antibacterial polysaccharide is β-glucan.

2. The bacteriostatic nursing cream according to claim 1, characterized in that, The β-glucan includes at least one of β-1,3-glucan, β-1,4-glucan or β-1,6-glucan.

3. The bacteriostatic nursing cream according to claim 1, characterized in that, The molecular weight of the β-glucan is 50 - 200 kDa.

4. The bacteriostatic nursing cream according to claim 1, wherein The softener includes at least one of polydimethylsiloxane or fatty acid ester compounds.

5. The bacteriostatic nursing cream according to claim 1, characterized in that, The surfactant includes at least one of cetylpyridinium chloride, ethyl lauroyl arginate or benzalkonium chloride.

6. An antibacterial nursing cream paper, characterized in that, Comprising the antibacterial nursing cream according to any one of claims 1 - 5.

7. The preparation method of an antibacterial nursing cream paper according to claim 6, characterized in that, Comprising the following preparation steps: Take glycerol and propylene glycol and place them in a reaction kettle, stir at low temperature and low speed, and collect the liquid phase; Add the antibacterial polysaccharide in portions and increase the temperature and speed for stirring to induce the self-assembly of the antibacterial polysaccharide to form a nanofiber gel liquid; Take the surfactant and spray it into the nanofiber gel liquid, then add the softener, cool down and stir, and collect the liquid antibacterial nursing cream; Take the antibacterial nursing cream and coat it on the surface of the virgin wood pulp paper until the moisture content is 25%, then the antibacterial nursing cream paper can be prepared.

8. The preparation method of an antibacterial nursing cream paper according to claim 7, characterized in that, The low temperature and low speed means stirring at a low speed of 200 rpm for 10 min at 40°C.

9. The preparation method of an antibacterial nursing cream paper according to claim 7, characterized in that, The coating amount of the antibacterial nursing cream is 10 - 20 g / m 2 .

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