Hair-fixing and hair-loss-preventing composition, application thereof and hair-fixing and hair-loss-preventing product

Abstract

The invention discloses a hair-fixing and hair-loss-preventing composition, application thereof and a hair-fixing and hair-loss-preventing product. The hair-fixing and hair-loss-preventing composition comprises 17-type collagen, a plant extract and a peptide substance, wherein the plant extract comprises a cacumen biotae extract. On the basis of inhibiting 5alpha-reductase and resisting oxidation, the composition disclosed by the invention also has the effects of fixing hair and preventing hair loss by virtue of ways of relieving inflammatory response, activating hair papilla cells, maintaining health of the hair papilla cells, reducing hair follicle senescence cells and the like.

Description

Technical Field

[0001] The present invention relates to the field of cosmetics, and particularly to a hair-fixing and anti-hair loss composition, its application, and a hair-fixing and anti-hair loss product. Background Art

[0002] The problem of hair loss is multi-dimensional, and its impact is not limited to the appearance level, but more widely involves multiple aspects such as mental health, social activities, economic status, and medical health. From a psychological perspective, hair loss can significantly reduce an individual's self-esteem and self-image perception, thereby inducing negative psychological states such as anxiety and depression, having an adverse impact on social activities, and in severe cases, may even lead to social isolation. At the same time, gender identity and personal attractiveness may also be impacted by hair loss. In the field of sociology, the negative impact of hair loss on career development cannot be ignored, especially in those professional fields with strict appearance requirements, where the impact is more significant. In addition, hair loss may also interfere with the evaluation of self-confidence and attractiveness in interpersonal relationships, reducing the overall quality of life. Economically, the financial burden brought about by hair loss treatment cannot be ignored, including the high costs of hair transplantation and special hair care products.

[0003] Hair loss is a complex physiological phenomenon affected by multiple factors. There are many causes of hair loss. External environments such as ultraviolet rays, chemical substances, and heat treatment can all damage the hair and exacerbate hair loss. Research shows that nutritional status is also crucial for hair health. Lack of nutrients such as protein, iron, zinc, and vitamins will directly affect hair growth and quality. At the same time, certain systemic diseases, such as thyroid diseases and autoimmune diseases, are also closely related to hair loss. Drug use is also a major inducement of hair loss. Many drugs such as chemotherapy drugs and antidepressants may cause hair loss. Scalp health problems cannot be underestimated either. Microbial imbalance, fungal infection, and inflammatory diseases can all lead to hair loss. Psychological factors such as stress and anxiety indirectly affect hair growth by influencing hormone levels and the immune system. Physical damage, poor hair care habits, and physiological processes such as pregnancy and childbirth may also cause hair loss. Finally, as people age, the hair growth cycle changes, which is also an important cause of hair loss.

[0004] Genetic factors are also crucial among the factors affecting hair loss. Especially in androgenetic alopecia, genetic susceptibility plays a decisive role. Changes in hormone levels cannot be ignored either. For example, an increase in androgens can trigger follicular atrophy, thereby leading to hair loss. Hair follicle stem cells play a key role in the hair growth cycle. The hair growth cycle includes the anagen, catagen, and telogen phases. Among them, hair follicle stem cells are activated during the anagen phase to promote new hair growth. The decline or disorder of hair follicle stem cell function can cause hair loss because it cannot maintain the anagen phase of the hair follicle, resulting in sparse and falling hair. Especially in androgenetic alopecia, androgens may interfere with hair follicle stem cells, triggering follicular atrophy and hair thinning.

[0005] At present, there are also a large number of anti-hair loss compositions and formulations on the market. Chinese Patent Application CN118845516A discloses an anti-hair loss composition, which is composed of adenosine, coffee extract, L-carnitine tartrate, copper tripeptide-1, Platycladus orientalis extract and niacinamide. This composition has significant synergistic effects in improving cell antioxidant capacity and inhibiting 5α-reductase. However, this composition has no obvious effect on inhibiting the senescence or disorder of hair follicle stem cells and maintaining the anagen phase of hair follicles. Summary of the Invention

[0006] In order to solve the above problems, the present invention provides a hair-fixing and anti-hair loss composition and its application, as well as a hair-fixing and anti-hair loss product. On the basis of inhibiting 5α-reductase and antioxidant, the composition of the present invention also exerts the effects of hair fixation and anti-hair loss through reducing inflammatory response, activating dermal papilla cells, maintaining the health of dermal papilla cells and reducing senescent cells in hair follicles.

[0007] To achieve the above technical objectives, the present invention provides the following technical solutions.

[0008] In a first aspect, the present invention provides a hair-fixing and anti-hair loss composition, which includes type XVII collagen, plant extract and peptide substances, and the plant extract includes Platycladus orientalis extract.

[0009] In the present invention, the type XVII collagen can be obtained by conventional chemical synthesis methods or genetic engineering methods in the art.

[0010] In some embodiments, the molecular weight of the type XVII collagen is 20-200 kDa, such as 23.8 kDa, 97 kDa, 120 kDa or 180 kDa.

[0011] In the present invention, the Platycladus orientalis extract can be obtained by extracting with the branches or branches and leaves of the plant Platycladus orientalis (L.) Franco of the family Cupressaceae. The extraction method can be conventional in the art.

[0012] In the present invention, the peptide substances can be selected from one or more of myristoyl pentapeptide-4, myristoyl pentapeptide-17, myristoyl tetrapeptide-12, myristoyl hexapeptide-16, acetyl tetrapeptide-3, oligopeptide-41, oligopeptide-74, copper acetylated peptide, biotin tripeptide-1, acetyl octapeptide-2, copper tripeptide-1, oligopeptide-1 and copper tripeptide-34.

[0013] Among them, the myristoyl pentapeptide-4 can be obtained by chemical synthesis method or genetic engineering method, and its chemical formula is C 37 H 71 N7O 10, with a molecular weight of 774.00 and a CAS No. of 1392416-25-9.

[0014] Among them, the acetyl tetrapeptide-3 can be obtained by chemical synthesis or genetic engineering methods, and its chemical formula is C 22 H 39 N9O5, with a molecular weight of 509.6 and a CAS No. of 827306-88-7.

[0015] Among them, the tripeptide-1 copper, also known as copper peptide, tripeptide, blue copper peptide, and copper peptide, can be obtained by chemical synthesis or genetic engineering methods, and its chemical formula is C 14 H 23 CuN6O4 + , with a molecular weight of 402.92.

[0016] Among them, the myristoyl hexapeptide-16 can be obtained by chemical synthesis or genetic engineering methods, and its chemical formula is C 41 H 81 N9O6, with a molecular weight of 796.14 and a CAS No. of 959610-30-1.

[0017] Among them, the oligopeptide-41 can be obtained by chemical synthesis or genetic engineering methods, and its chemical formula is C 63 H 90 N 18 O 19 S, with a molecular weight of 1435.56.

[0018] Among them, the acetylated copper peptide can be obtained by chemical synthesis or genetic engineering methods, and its chemical formula is C 30 H 48 CuN 12 O 10 , with a molecular weight of 800.33 and a CAS No. of 130120-57-9.

[0019] Among them, the acetyl octapeptide-2 can be obtained by chemical synthesis or genetic engineering methods, and its chemical formula is C 44 H 81 N 13 O 14 , with a molecular weight of 1016.20.

[0020] In some embodiments, in the hair-fixing and anti-hair loss composition, the peptide substances include one or more of myristoyl pentapeptide-4, acetyl tetrapeptide-3, and tripeptide-1 copper.

[0021] In some embodiments, the hair-fixing and anti-hair loss composition includes type 17 collagen, Platycladus orientalis leaf extract, and myristoyl pentapeptide-4.

[0022] In some embodiments, the hair-fixing and anti-hair-loss composition consists of collagen type XVII, Platycladus orientalis leaf extract, and myristoyl pentapeptide-4.

[0023] In the above embodiments, preferably, the mass ratio of the collagen type XVII, Platycladus orientalis leaf extract, and myristoyl pentapeptide-4 is (0.0001 - 0.1):(0.1 - 5):(0.001 - 3), for example, 1:100:1.

[0024] In some embodiments, the hair-fixing and anti-hair-loss composition includes collagen type XVII, Platycladus orientalis leaf extract, and acetyl tetrapeptide-3.

[0025] In some embodiments, the hair-fixing and anti-hair-loss composition consists of collagen type XVII, Platycladus orientalis leaf extract, and acetyl tetrapeptide-3.

[0026] In the above embodiments, preferably, the mass ratio of the collagen type XVII, Platycladus orientalis leaf extract, and acetyl tetrapeptide-3 is (0.0001 - 0.1):(0.1 - 5):(0.003 - 0.5), for example, 1:100:3.

[0027] In some embodiments, the hair-fixing and anti-hair-loss composition includes collagen type XVII, Platycladus orientalis leaf extract, and tripeptide-1 copper.

[0028] In some embodiments, the hair-fixing and anti-hair-loss composition consists of collagen type XVII, Platycladus orientalis leaf extract, and tripeptide-1 copper.

[0029] In the above embodiments, preferably, the mass ratio of the collagen type XVII, Platycladus orientalis leaf extract, and tripeptide-1 copper is (0.0001 - 0.1):(0.1 - 5):(0.001 - 0.5), for example, 1:100:10.

[0030] In some embodiments, the hair-fixing and anti-hair-loss composition includes collagen type XVII, Platycladus orientalis leaf extract, myristoyl pentapeptide-4, and acetyl tetrapeptide-3.

[0031] In some embodiments, the hair-fixing and anti-hair-loss composition consists of collagen type XVII, Platycladus orientalis leaf extract, myristoyl pentapeptide-4, and acetyl tetrapeptide-3.

[0032] In the above embodiments, preferably, the mass ratio of the collagen type XVII, Platycladus orientalis leaf extract, myristoyl pentapeptide-4, and acetyl tetrapeptide-3 is (0.0001 - 0.1):(0.1 - 5):(0.001 - 3):(0.003 - 0.5), for example, 1:100:1:3.

[0033] In some embodiments, the hair-fixing and anti-hair loss composition comprises collagen type XVII, Platycladus orientalis leaf extract, myristoyl pentapeptide-4, and tripeptide-1 copper.

[0034] In some embodiments, the hair-fixing and anti-hair loss composition consists of collagen type XVII, Platycladus orientalis leaf extract, myristoyl pentapeptide-4, and tripeptide-1 copper.

[0035] In the above embodiments, preferably, the mass ratio of the collagen type XVII, Platycladus orientalis leaf extract, myristoyl pentapeptide-4, and tripeptide-1 copper is (0.0001 - 0.1):(0.1 - 5):(0.001 - 3):(0.001 - 0.5), for example, 1:100:1:10.

[0036] In some embodiments, the hair-fixing and anti-hair loss composition comprises collagen type XVII, Platycladus orientalis leaf extract, myristoyl pentapeptide-4, acetyl tetrapeptide-3, and tripeptide-1 copper.

[0037] In some embodiments, the hair-fixing and anti-hair loss composition consists of collagen type XVII, Platycladus orientalis leaf extract, myristoyl pentapeptide-4, acetyl tetrapeptide-3, and tripeptide-1 copper.

[0038] In the above embodiments, preferably, the mass ratio of the collagen type XVII, Platycladus orientalis leaf extract, myristoyl pentapeptide-4, acetyl tetrapeptide-3, and tripeptide-1 copper is (0.0001 - 0.1):(0.1 - 5):(0.001 - 3):(0.003 - 0.5):(0.001 - 0.5), for example, 1:100:1:3:10.

[0039] In some embodiments, the hair-fixing and anti-hair loss composition comprises collagen type XVII, Platycladus orientalis leaf extract, and myristoyl hexapeptide-16.

[0040] In some embodiments, the hair-fixing and anti-hair loss composition consists of collagen type XVII, Platycladus orientalis leaf extract, and myristoyl hexapeptide-16.

[0041] In the above embodiments, preferably, the mass ratio of the collagen type XVII, Platycladus orientalis leaf extract, and myristoyl hexapeptide-16 is (0.0001 - 0.1):(0.1 - 5):(0.001 - 0.5), for example, 1:100:10.

[0042] In some embodiments, the hair-fixing and anti-hair loss composition comprises collagen type XVII, Platycladus orientalis leaf extract, and oligopeptide-41.

[0043] In some embodiments, the hair-fixing and anti-hair loss composition consists of collagen type XVII, Platycladus orientalis leaf extract, and oligopeptide-41.

[0044] In the above embodiments, preferably, the mass ratio of type XVII collagen, Platycladus orientalis extract, and oligopeptide-41 is (0.0001-0.1):(0.1-5):(0.001-0.5), for example, 1:100:10.

[0045] In some embodiments, the hair-fixing and anti-hair loss composition includes type XVII collagen, Platycladus orientalis extract, and copper acetopeptide.

[0046] In some embodiments, the hair-fixing and anti-hair loss composition consists of type XVII collagen, Platycladus orientalis extract, and copper acetopeptide.

[0047] In the above embodiments, preferably, the mass ratio of type XVII collagen, Platycladus orientalis extract, and copper acetopeptide is (0.0001-0.1):(0.1-5):(0.001-0.5), for example, 1:100:10.

[0048] In some embodiments, the hair-fixing and anti-hair loss composition includes type XVII collagen, Platycladus orientalis extract, and acetyl octapeptide-2.

[0049] In some embodiments, the hair-fixing and anti-hair loss composition consists of type XVII collagen, Platycladus orientalis extract, and acetyl octapeptide-2.

[0050] In the above embodiments, preferably, the mass ratio of type XVII collagen, Platycladus orientalis extract, and acetyl octapeptide-2 is (0.0001-0.1):(0.1-5):(0.001-0.5), for example, 1:100:10.

[0051] In a second aspect, the present invention provides a hair-fixing and anti-hair loss product, which includes the hair-fixing and anti-hair loss composition as described above.

[0052] In some embodiments, the hair-fixing and anti-hair loss product is shampoo, hair conditioner, hair essence, body wash, or body lotion.

[0053] In a third aspect, the present invention provides the use of the hair-fixing and anti-hair loss composition as described above in the preparation of a hair-fixing and anti-hair loss product.

[0054] On the basis of conforming to common general knowledge in the art, the above preferred conditions can be combined arbitrarily to obtain various preferred examples of the present invention.

[0055] The reagents and raw materials used in the present invention are all commercially available.

[0056] The positive and progressive effects of the present invention are as follows:

[0057] 1. The present invention uses type XVII collagen, Platycladus orientalis leaf extract, and peptide substances in combination. Based on inhibiting 5α-reductase and antioxidation, it also exerts the effects of hair strengthening and hair loss prevention by activating dermal papilla cells, maintaining the health of dermal papilla cells, and reducing follicular senescent cells. In addition, the composition of the present invention also has the effect of reducing inflammatory responses.

[0058] 2. The composition of the present invention can provide nutrients for all stages of hair growth. The type XVII collagen and peptide substances such as myristoyl pentapeptide-4, acetyl tetrapeptide-3, and tripeptide-1 copper in the composition also have the functions of promoting collagen synthesis, moisturizing the scalp, and regulating scalp blood flow.

[0059] 3. The composition materials of the present invention are easily available, safe, healthy, and have stable efficacy, and are suitable for large-scale industrial production. BRIEF DESCRIPTION OF THE DRAWINGS

[0060] Figure 1 Schematic diagram of the test results of the 5α-reductase inhibition rate test of different compositions in each example.

[0061] Figure 2 Schematic diagram of the test results of the antioxidant capacity test of different compositions in each example.

[0062] Figure 3 Schematic diagram of the test results of the ability to promote COL17 expression of different compositions in each example.

[0063] Figure 4 Schematic diagram of the test results of the ability to promote TGF-β2 expression of different compositions in each example.

[0064] Figure 5 Schematic diagram of the test results of the ability to promote VEGFA expression of different compositions in each example.

[0065] Figure 6 Schematic diagram of the test results of the ability to reduce follicular senescent cells of different compositions in each example. DETAILED DESCRIPTION OF THE INVENTION

[0066] The present invention will be further described below by way of examples, but the present invention is not limited to the scope of the described examples. For the experimental methods without specific conditions in the following examples, they are carried out according to conventional methods and conditions, or selected according to the product specifications.

[0067] Raw materials such as type XVII collagen, Platycladus orientalis leaf extract, myristoyl pentapeptide-4, acetyl tetrapeptide-3, tripeptide-1 copper, myristoyl hexapeptide-16, oligopeptide-41, copper acetopeptide, and acetyl octapeptide-2 are all purchased from raw material suppliers.

[0068] The manufacturer and model information of each raw material component is as follows:

[0069]

[0070]

[0071] Preparation of the hair-fixing and anti-hair-loss composition 1 in Example 1

[0072] Mix 17-type collagen, Platycladus orientalis leaf extract, and myristoyl pentapeptide-4 in a mass ratio of 1:100:1 to obtain the hair-fixing and anti-hair-loss composition 1.

[0073] For subsequent tests, dissolve the hair-fixing and anti-hair-loss composition 1 in water to prepare an aqueous solution, and the contents of 17-type collagen, Platycladus orientalis leaf extract, and myristoyl pentapeptide-4 in the solution are 0.001%, 0.1%, and 0.001% respectively.

[0074] Preparation of the hair-fixing and anti-hair-loss composition 2 in Example 2

[0075] Mix 17-type collagen, Platycladus orientalis leaf extract, and acetyl tetrapeptide-3 in a mass ratio of 1:100:3 to obtain the hair-fixing and anti-hair-loss composition 2.

[0076] For subsequent tests, dissolve the hair-fixing and anti-hair-loss composition 2 in water to prepare an aqueous solution, and the contents of 17-type collagen, Platycladus orientalis leaf extract, and acetyl tetrapeptide-3 in the solution are 0.001%, 0.1%, and 0.003% respectively.

[0077] Preparation of the hair-fixing and anti-hair-loss composition 3 in Example 3

[0078] Mix 17-type collagen, Platycladus orientalis leaf extract, and tripeptide-1 copper in a mass ratio of 1:100:10 to obtain the hair-fixing and anti-hair-loss composition 3.

[0079] For subsequent tests, dissolve the hair-fixing and anti-hair-loss composition 3 in water to prepare an aqueous solution, and the contents of 17-type collagen, Platycladus orientalis leaf extract, and tripeptide-1 copper in the solution are 0.001%, 0.1%, and 0.01% respectively.

[0080] Preparation of the hair-fixing and anti-hair-loss composition 4 in Example 4

[0081] Mix 17-type collagen, Platycladus orientalis leaf extract, and myristoyl hexapeptide-16 in a mass ratio of 1:100:10 to obtain the hair-fixing and anti-hair-loss composition 4.

[0082] For subsequent tests, dissolve the hair-fixing and anti-hair-loss composition 4 in water to prepare an aqueous solution, and the contents of 17-type collagen, Platycladus orientalis leaf extract, and myristoyl hexapeptide-16 in the solution are 0.001%, 0.1%, and 0.01% respectively.

[0083] Preparation of Hair-Strengthening and Anti-Hair Loss Composition 5

[0084] Mix 17 - type collagen, Platycladus orientalis leaf extract, and oligopeptide - 41 in a mass ratio of 1:100:10 to obtain Hair-Strengthening and Anti-Hair Loss Composition 5.

[0085] For subsequent tests, dissolve Hair-Strengthening and Anti-Hair Loss Composition 5 in water to prepare an aqueous solution, where the contents of 17 - type collagen, Platycladus orientalis leaf extract, and oligopeptide - 41 in the solution are 0.001%, 0.1%, and 0.01% respectively.

[0086] Preparation of Hair-Strengthening and Anti-Hair Loss Composition 6

[0087] Mix 17 - type collagen, Platycladus orientalis leaf extract, and cupric peptide acetate in a mass ratio of 1:100:10 to obtain Hair-Strengthening and Anti-Hair Loss Composition 6.

[0088] For subsequent tests, dissolve Hair-Strengthening and Anti-Hair Loss Composition 6 in water to prepare an aqueous solution, where the contents of 17 - type collagen, Platycladus orientalis leaf extract, and cupric peptide acetate in the solution are 0.001%, 0.1%, and 0.01% respectively.

[0089] Preparation of Hair-Strengthening and Anti-Hair Loss Composition 7

[0090] Mix 17 - type collagen, Platycladus orientalis leaf extract, and acetyl octapeptide - 2 in a mass ratio of 1:100:10 to obtain Hair-Strengthening and Anti-Hair Loss Composition 7.

[0091] For subsequent tests, dissolve Hair-Strengthening and Anti-Hair Loss Composition 7 in water to prepare an aqueous solution, where the contents of 17 - type collagen, Platycladus orientalis leaf extract, and acetyl octapeptide - 2 in the solution are 0.001%, 0.1%, and 0.01% respectively.

[0092] Preparation of Hair-Strengthening and Anti-Hair Loss Composition 8

[0093] Mix 17 - type collagen, Platycladus orientalis leaf extract, myristoyl pentapeptide - 4, and acetyl tetrapeptide - 3 in a mass ratio of 1:100:1:3 to obtain Hair-Strengthening and Anti-Hair Loss Composition 8.

[0094] For subsequent tests, dissolve Hair-Strengthening and Anti-Hair Loss Composition 8 in water to prepare an aqueous solution, where the contents of 17 - type collagen, Platycladus orientalis leaf extract, myristoyl pentapeptide - 4, and acetyl tetrapeptide - 3 in the solution are 0.001%, 0.1%, 0.001%, and 0.003% respectively.

[0095] Preparation of Hair-Strengthening and Anti-Hair Loss Composition 9

[0096] Mix type XVII collagen, Platycladus orientalis leaf extract, Myristoyl Pentapeptide-4, and Copper Tripeptide-1 in a mass ratio of 1:100:1:10 to obtain the hair-fixing and anti-hair loss composition 9.

[0097] For subsequent tests, dissolve the hair-fixing and anti-hair loss composition 9 in water to prepare an aqueous solution, where the contents of type XVII collagen, Platycladus orientalis leaf extract, Myristoyl Pentapeptide-4, and Copper Tripeptide-1 in the solution are 0.001%, 0.1%, 0.001%, and 0.01% respectively.

[0098] Example 10 Preparation of the hair-fixing and anti-hair loss composition 10

[0099] Mix type XVII collagen, Platycladus orientalis leaf extract, Myristoyl Pentapeptide-4, Acetyl Tetrapeptide-3, and Copper Tripeptide-1 in a mass ratio of 1:100:1:3:10 to obtain the hair-fixing and anti-hair loss composition 10.

[0100] For subsequent tests, dissolve the hair-fixing and anti-hair loss composition 10 in water to prepare an aqueous solution, where the contents of type XVII collagen, Platycladus orientalis leaf extract, Myristoyl Pentapeptide-4, Acetyl Tetrapeptide-3, and Copper Tripeptide-1 in the solution are 0.001%, 0.1%, 0.001%, 0.003%, and 0.01% respectively.

[0101] Comparative Example 1 Preparation of Comparative Composition 1

[0102] Mix type XVII collagen, Magnolia officinalis extract, and Myristoyl Pentapeptide-4 in a mass ratio of 1:100:1 to obtain Comparative Composition 1.

[0103] For subsequent tests, dissolve Comparative Composition 1 in water to prepare an aqueous solution. The contents of type XVII collagen, Magnolia officinalis extract, and Myristoyl Pentapeptide-4 in the solution are 0.001%, 0.1%, and 0.001% respectively.

[0104] Comparative Example 2 Preparation of Comparative Composition 2

[0105] Mix type XVII collagen, Polygonum multiflorum extract, and Myristoyl Pentapeptide-4 in a mass ratio of 1:100:1 to obtain Comparative Composition 2.

[0106] For subsequent tests, dissolve Comparative Composition 2 in water to prepare an aqueous solution. The contents of type XVII collagen, Polygonum multiflorum extract, and Myristoyl Pentapeptide-4 in the solution are 0.001%, 0.1%, and 0.001% respectively.

[0107] Comparative Example 3 Preparation of Comparative Composition 3

[0108] Mix type XVII collagen, ginger extract, and myristoyl pentapeptide-4 in a mass ratio of 1:100:1 to obtain Comparative Composition 3.

[0109] For subsequent tests, dissolve Comparative Composition 3 in water to prepare an aqueous solution. In the aqueous solution, the contents of type XVII collagen, ginger extract, and myristoyl pentapeptide-4 are 0.001%, 0.1%, and 0.001% respectively.

[0110] Preparation of Comparative Composition 4 in Comparative Example 4

[0111] Mix type XVII collagen, coffee extract, and myristoyl pentapeptide-4 in a mass ratio of 1:2.5:1 to obtain Comparative Composition 4.

[0112] For subsequent tests, dissolve Comparative Composition 4 in water to prepare an aqueous solution. In the aqueous solution, the contents of type XVII collagen, coffee extract, and myristoyl pentapeptide-4 are 0.001%, 0.0025%, and 0.001% respectively.

[0113] Preparation of Comparative Composition 5 in Comparative Example 5

[0114] Mix type XVII collagen, calamus extract, and myristoyl pentapeptide-4 in a mass ratio of 1:100:1 to obtain Comparative Composition 5.

[0115] For subsequent tests, dissolve Comparative Composition 5 in water to prepare an aqueous solution. In the aqueous solution, the contents of type XVII collagen, calamus extract, and myristoyl pentapeptide-4 are 0.001%, 0.1%, and 0.001% respectively.

[0116] Effect Example 1: 5α-Reductase Inhibition Rate Test

[0117] 1. Test Procedure

[0118] (1) Sample Tube: Add 1 mL of sample solution, 1 mL of enzyme solution, 1 mL of NADPH solution, and 1 mL of testosterone solution into a test tube, shake gently, pipette 200 μL into a 96-well microplate. Do three parallels for each sample and place it in a microplate reader for detection. Measure the absorbance at 340 nm, which is the first measured value A. 样品0 After incubating the sample at 37 °C for 20 minutes, place it in a microplate reader for detection. Measure the absorbance at 340 nm, which is the second measured value A. 样品20 .

[0119] (2) Enzyme tube: Add 1 mL of PBS solution, 1 mL of enzyme solution, 1 mL of NADPH solution, and 1 mL of testosterone solution into a test tube, gently shake well, pipette 200 μL into a 96-well microplate, make three parallels for each sample, place it in a microplate reader for detection, and measure the absorbance at 340 nm, which is the first measured value A. 酶0 After incubating the sample at 37 °C for 20 minutes, place it in a microplate reader for detection, and measure the absorbance at 340 nm, which is the second measured value A. 酶20 ; At the same time, perform blank and positive control detections.

[0120] (3) The 5α-reductase inhibition rate is calculated according to the following formula:

[0121]

[0122] Each sample is tested three times, and the average value is taken and recorded in Table 1.

[0123] 2. Test results

[0124] Table 1 Test results of 5α-reductase inhibition rate

[0125]

[0126]

[0127] The experimental results are shown in Table 1 and Figure 1 as follows. Compared with Components 1-9, Examples 1-10 have significantly improved in inhibiting 5α-reductase activity; among all the compositions, Example 10 has the strongest ability to inhibit 5α-reductase activity. Compared with Comparative Examples 1-5, Example 1 has a stronger ability to inhibit 5α-reductase activity. It can be seen that compared with other plant extracts, type 17 collagen and myristoyl pentapeptide-4 have a better cooperative effect with Platycladus orientalis extract.

[0128] Effect Example 2: Test on the antioxidant ability of the composition

[0129] 1. Test process

[0130] (1) Inoculation of dermal papilla cells: Inoculate cells into a 6-well plate at an inoculation density of 3×10 5 cells / well, and place the 6-well plate in an incubator (37 °C, 5% CO2) for overnight incubation.

[0131] (2) Solution preparation: Prepare test substances with different concentrations according to different test groups.

[0132] (3) H2O2 stimulation: According to the test group, when the cell confluence rate in the 6-well plate reaches about 60%, perform H2O2 stimulation, and the induction time is 1 hour.

[0133] (4) Administration: After the induction time ended, administration was carried out according to the test grouping. 2 mL of sample was added to each well, and 3 replicate wells were set for each group. Incubate in an incubator at 37 °C with 5% CO2 for 23 hours.

[0134] (5) Reactive oxygen species (ROS) detection: After incubation, 1 mL of DCFH-DA probe with a concentration of 10 μM was added to each well and incubated in an incubator (37 °C, 5% CO2) for 30 minutes; discard the culture medium containing DCFH-DA, wash 3 times with PBS, digest the cells with trypsin (0.25%), wash the cells 1 time with PBS, add a certain amount of fresh PBS, and detect by flow cytometry.

[0135] (6) The ROS inhibition rate was calculated according to the following formula:

[0136]

[0137] For each sample, the average value of the test results of 3 replicate wells was taken and recorded in Table 2.

[0138] 2. Test results

[0139] Table 2 Test results of antioxidant capacity

[0140]

[0141]

[0142] The experimental results are shown in Table 2 and Figure 2 as follows. After treatment with components 1-7 and 9 at the above concentrations respectively, the content of reactive oxygen species (ROS) decreased slightly, and the ROS inhibition rate did not exceed 22%. After treatment with component 8 at the above concentrations respectively, the ROS content did not decrease but increased. After treatment with Examples 1-10 at the above concentrations respectively, the ROS content decreased significantly. Among them, Example 8 had the strongest ability to inhibit ROS. Compared with Comparative Examples 1-5, Example 1 had a stronger ability to inhibit ROS. It can be seen that compared with other plant extracts, type XVII collagen and myristoyl pentapeptide-4 have a better synergistic effect with Platycladus orientalis extract.

[0143] Effect Example 3: Test on the ability to promote the expression of COL17, TGF-β2 and VEGFA

[0144] 1. Test procedure

[0145] (1) Seed dermal papilla cells at a density of 1.5×10 5 cells per well in a 6-well plate and incubate overnight in a CO2 incubator (37 °C, 5% CO2).

[0146] (2) Administration and stimulation. Administration and stimulation were carried out according to the test groups, with administration and stimulation being carried out simultaneously, and 3 replicate wells were set in each group. In the BC (blank control) group, 2 mL of culture medium was added to each well. In the NC (negative control) group, 2 mL of culture medium containing H2O2 was added to each well. In the PC (positive control) group, 2 mL of culture medium containing H2O2 and WY14643 was added to each well. In the sample group, 2 mL of culture medium containing H2O2 and the test sample at the corresponding concentration was added to each well. After completion, all 6-well plates were transferred to a CO2 incubator (37 °C, 5% CO2) and incubated for 24 hours.

[0147] (3) After incubation, discard the old liquid, wash twice with 1 mL / well of PBS, add 1 mL of AG RNAex Pro Reagent to each well, pipette to lyse the cells, and then collect the samples. RNA was extracted, reverse transcribed into cDNA, and then subjected to fluorescence quantitative PCR detection, and the 2 -△△CT method was used for calculation.

[0148] (4) Calculation of upregulation rate:

[0149]

[0150] For each sample, the average value of the test results of 3 replicate wells was taken and recorded in Tables 3 - 5.

[0151] 2. Test results

[0152] Table 3 COL17 expression results

[0153]

[0154]

[0155] The experimental results are shown in Table 3 and Figure 3 as follows. After treatment with the above concentrations, the upregulation rate of the COL 17 gene expression was 44.23% for Component 1 (collagen type XVII), 17.31% for Component 2 (extract of Platycladus orientalis leaves). After treatment with the above concentrations, the COL 17 gene expression of Components 3 and 5 - 9 increased slightly, but the upregulation rate did not exceed 10%. After treatment with the above concentrations respectively, the COL 17 gene expression of Component 4 did not increase.

[0156] After treatment with the above concentrations respectively, the COL 17 gene expression in Examples 1 - 10 increased significantly, and the upregulation rate ranged from 67.31% to 117.31%. Among them, Example 9 had the largest upregulation rate of the COL 17 gene expression. Compared with Comparative Examples 1 - 5, Example 1 had a greater upregulation rate of the COL 17 gene expression. It can be seen that compared with other plant extracts, collagen type XVII and myristoyl pentapeptide - 4 have a better cooperative effect with the extract of Platycladus orientalis leaves.

[0157] Table 4 TGF-β2 expression results

[0158]

[0159]

[0160] As shown in Table 4 and Figure 4 as shown, after treatment with the above concentrations, the up-regulation rate of TGF-β2 gene expression in Component 1 (Collagen type XVII) was 144.81%, and the up-regulation rate of TGF-β2 gene expression in Component 2 (Platycladus orientalis extract) was 38.31% after treatment with the above concentrations; after treatment with the above concentrations of Components 3-9, the TGF-β2 gene expression increased slightly, but the up-regulation rate did not exceed 20%.

[0161] After the above concentrations of Examples 1-10 were treated respectively, the TGF-β2 gene expression increased significantly, and the up-regulation rate was about 300%, and even reached 364.29%. Among them, Example 10 had the largest up-regulation rate of TGF-β2 gene expression. Compared with Comparative Examples 1-5, Example 1 had a greater up-regulation rate of TGF-β2 gene expression. It can be seen that compared with other plant extracts, Collagen type XVII and Myristoyl Pentapeptide-4 have a better cooperative effect with Platycladus orientalis extract. The increase in TGF-β2 gene expression can promote the transformation of hair follicle stem cells from the quiescent phase to the growth phase, which is more conducive to hair fixation.

[0162] Table 5 VEGFA expression results

[0163]

[0164]

[0165] As shown in Table 5 and Figure 5 as shown, after treatment with the above concentrations, the up-regulation rate of VEGFA gene expression in Component 1 (Collagen type XVII) was 128.16%, and the up-regulation rate of VEGFA gene expression in Component 2 (Platycladus orientalis extract) was 63.79% after treatment with the above concentrations; after treatment with the above concentrations of Components 3-9, the VEGFA gene expression increased slightly, but the up-regulation rate did not exceed 23%.

[0166] After the above concentrations of Examples 1-10 were treated respectively, the VEGFA gene expression increased significantly, and the up-regulation rate ranged from 166.09% to 254.60%. Among them, Example 10 had the largest up-regulation rate of VEGFA gene expression. Compared with Comparative Examples 1-5, Example 1 had a greater up-regulation rate of VEGFA gene expression. It can be seen that compared with other plant extracts, Collagen type XVII and Myristoyl Pentapeptide-4 have a better cooperative effect with Platycladus orientalis extract.

[0167] Effect Example 4: Test on the ability to reduce follicular senescent cells

[0168] 1. Test procedure

[0169] (1) Seed dermal papilla cells at a density of 1.5×10 5 cells per well in a 6-well plate and incubate overnight in a CO2 incubator (37°C, 5% CO2).

[0170] (2) Administration of drugs and stimulation. According to the test groups, administer drugs and perform stimulation simultaneously, with 3 replicate wells in each group. In the BC (blank control) group, add 2 mL of culture medium to each well; in the NC (negative control) group, add 2 mL of culture medium containing H2O2 to each well; in the PC (positive control) group, add 2 mL of culture medium containing H2O2 and WY14643 to each well; in the sample group, add culture medium containing H2O2 and the test sample at the corresponding concentration to each well. After completion, transfer all 6-well plates to a CO2 incubator (37°C, 5% CO2) and incubate for 24 hours.

[0171] (3) Use a β-galactosidase staining kit to stain the cells after treatment with the test substance, and count the proportion of senescent cells among all cells.

[0172] For each sample, take the average of the test results from 3 replicate wells and record it in Table 6.

[0173] 2. Test results

[0174] Table 6 Test results on the ability of the composition to reduce follicular senescent cells

[0175]

[0176]

[0177] The experimental results are shown in Table 6 and Figure 6 as follows. Compared with Components 1-9, Examples 1-10 showed a significant improvement in inhibiting the senescence of dermal papilla cells; among all the compositions, Example 3 had the strongest ability to inhibit the senescence of dermal papilla cells. Compared with Comparative Examples 1-5, Example 1 had a stronger ability to inhibit the senescence of dermal papilla cells. It can be seen that compared with other plant extracts, type XVII collagen and myristoyl pentapeptide-4 have a better synergistic effect with Platycladus orientalis extract.

[0178] Application Example 1: Preparation of a hair care essence

[0179] Prepare a hair care essence using the anti-hair loss and hair strengthening composition of Example 1 according to the formula in Table 7. The preparation process is as follows:

[0180] Add raw material water and dipropylene glycol into the main pot respectively, stir evenly, then add type 17 collagen, and stir until completely dissolved; then add myristoyl pentapeptide-4 and stir evenly; next, premix the extract of Platycladus orientalis leaves with a certain amount of water first, after premixing evenly, add it into the main pot and stir evenly; then add dipotassium glycyrrhizinate and VE derivative, and stir evenly; test the pH of the material, and add arginine to adjust the pH to 6-7.

[0181] Table 7

[0182] raw material concentration Collagen Type XVII 0.02％ extract of Platycladus orientalis 2.0％ Myristoyl Pentapeptide-4 0.02％ Dipropylene Glycol 5％ Dipotassium Glycyrrhizinate 0.5％ VE derivative 0.4％ arginine 1.0％ water balance

[0183] Conduct the efficacy test of hair strengthening and anti-hair loss through mouse experiments. Mice in the telogen phase (skin is pink) were selected for the experiment. Take 18 male SPF-grade C57BL / 6 mice, 6-7 weeks old. After 7 days of adaptive feeding of the mice, they were randomly divided into 3 groups according to their body weights, namely the normal control group, the positive control group, and the test article group, with 6 mice in each group. Under the anesthesia of the mice, select an area of about 2 cm×3 cm on their backs as the depilation area, and the long side of this area is parallel to the spine. Use a hair clipper to shave the hair in this area, and further depilate it with depilatory cream. After pre-hair removal, the mice in the test article group were treated with the test article (hair care essence of Application Example 1) by skin application, and the intervention period was 21 days. The mice in the normal control group were smeared with normal saline, and the positive control group was smeared with a commercially available competitor product (5% minoxidil). The specific grouping and administration information are shown in Table 8:

[0184] Table 8

[0185]

[0186]

[0187] Mice in each group were photographed every day before and after the intervention to record the color of the skin and hair in the depilation area. And the hair growth of the mice was scored on the 3rd, 7th, 10th, 14th, and 21st days before and after the intervention. The hair scoring criteria are shown in Table 9:

[0188] Table 9

[0189] score description 0 No hair growth was observed in the hair removal area 1 Hair growth in ≤20% area 2 Hair growth in 20% - 40% area 3 Hair growth in 40% - 60% area 4 Hair growth in 60% - 80% area 5 Hair growth in >80% area

[0190] After the administration, the hair quality and hair length of the newly grown hair in the depilation area of each group of mice were detected. After removing the newly grown hair in the depilation area, it was weighed, and the hair quality was recorded per square centimeter of skin. In addition, 50 hairs were randomly selected from each mouse, fixed with transparent tape, and the length of the newly grown hair in the depilation area was measured with a ruler, and the measurement results were recorded.

[0191] The test results showed that, compared with the normal control group and the positive control group, the mice using the hair care essence of the present invention had faster hair growth. By 14 days, hair growth had occurred in more than 80% of the area, and the hair was longer and heavier.

[0192] Although the specific embodiments of the present invention have been described above, those skilled in the art should understand that this is only an example, and the protection scope of the present invention is defined by the appended claims. Without departing from the principles and essence of the present invention, those skilled in the art can make various changes or modifications to these embodiments, but these changes and modifications all fall within the protection scope of the present invention.

Claims

1. A hair-fixing and anti-hair-loss composition, characterized in that, The hair strengthening and anti - hair loss composition includes type XVII collagen, plant extracts and peptide substances, and the plant extracts include Platycladus orientalis leaf extract.

2. The hair-fixing and anti-hair loss composition according to claim 1, wherein The molecular weight of the type XVII collagen is 20 - 200 kDa, such as 23.8 kDa, 97 kDa, 120 kDa or 180 kDa.

3. The hair-fixing and anti-hair-loss composition according to claim 1, wherein The mass ratio of the type XVII collagen, the Platycladus orientalis leaf extract and the peptide substances is (0.0001 - 0.1):(0.1 - 5):(0.001 - 3).

4. The hair-fixing and anti-hair-loss composition according to claim 1, wherein, The peptide substances are selected from one or more of myristoyl pentapeptide - 4, myristoyl pentapeptide - 17, myristoyl tetrapeptide - 12, myristoyl hexapeptide - 16, acetyl tetrapeptide - 3, oligopeptide - 41, oligopeptide - 74, copper acetopeptide, biotin tripeptide - 1, acetyl octapeptide - 2, tripeptide - 1 copper, oligopeptide - 1 and tripeptide - 34 copper.

5. The hair-fixing and anti-hair loss composition according to any one of claims 1-4, characterized in that, The hair strengthening and anti - hair loss composition includes type XVII collagen, Platycladus orientalis leaf extract and myristoyl pentapeptide - 4; preferably, the mass ratio of the type XVII collagen, the Platycladus orientalis leaf extract and myristoyl pentapeptide - 4 is (0.0001 - 0.1):(0.1 - 5):(0.001 - 3), such as 1:100:1; Or, the hair strengthening and anti - hair loss composition includes type XVII collagen, Platycladus orientalis leaf extract and acetyl tetrapeptide - 3; preferably, the mass ratio of the type XVII collagen, the Platycladus orientalis leaf extract and acetyl tetrapeptide - 3 is (0.0001 - 0.1):(0.1 - 5):(0.003 - 0.5), such as 1:100:3; Or, the hair strengthening and anti - hair loss composition includes type XVII collagen, Platycladus orientalis leaf extract and tripeptide - 1 copper; preferably, the mass ratio of the type XVII collagen, the Platycladus orientalis leaf extract and tripeptide - 1 copper is (0.0001 - 0.1):(0.1 - 5):(0.001 - 0.5), such as 1:100:

10.

6. The hair-fixing and anti-hair loss composition according to any one of claims 1-4, characterized in that, The hair strengthening and anti - hair loss composition includes type XVII collagen, Platycladus orientalis leaf extract, myristoyl pentapeptide - 4 and acetyl tetrapeptide - 3; preferably, the mass ratio of the type XVII collagen, the Platycladus orientalis leaf extract, myristoyl pentapeptide - 4 and acetyl tetrapeptide - 3 is (0.0001 - 0.1):(0.1 - 5):(0.001 - 3):(0.003 - 0.5), such as 1:100:1:3; Or, the hair strengthening and anti - hair loss composition includes type XVII collagen, Platycladus orientalis leaf extract, myristoyl pentapeptide - 4 and tripeptide - 1 copper; preferably, the mass ratio of the type XVII collagen, the Platycladus orientalis leaf extract, myristoyl pentapeptide - 4 and tripeptide - 1 copper is (0.0001 - 0.1):(0.1 - 5):(0.001 - 3):(0.001 - 0.5), such as 1:100:1:10; Alternatively, the hair strengthening and anti - hair loss composition comprises type XVII collagen, Platycladus orientalis leaf extract, myristoyl pentapeptide - 4, acetyl tetrapeptide - 3 and tripeptide - 1 copper; preferably, the mass ratio of type XVII collagen, Platycladus orientalis leaf extract, myristoyl pentapeptide - 4, acetyl tetrapeptide - 3 and tripeptide - 1 copper is (0.0001 - 0.1):(0.1 - 5):(0.001 - 3):(0.003 - 0.5):(0.001 - 0.5), for example, 1:100:1:3:

10.

7. The hair-fixing and anti-hair loss composition according to any one of claims 1-4, characterized in that, The hair strengthening and anti - hair loss composition comprises type XVII collagen, Platycladus orientalis leaf extract and myristoyl hexapeptide - 16; preferably, the mass ratio of type XVII collagen, Platycladus orientalis leaf extract and myristoyl hexapeptide - 16 is (0.0001 - 0.1):(0.1 - 5):(0.001 - 0.5), for example, 1:100:10; Alternatively, the hair strengthening and anti - hair loss composition comprises type XVII collagen, Platycladus orientalis leaf extract and oligopeptide - 41; preferably, the mass ratio of type XVII collagen, Platycladus orientalis leaf extract and oligopeptide - 41 is (0.0001 - 0.1):(0.1 - 5):(0.001 - 0.5), for example, 1:100:

10.

8. The hair-fixing and anti-hair loss composition according to any one of claims 1-4, characterized in that, The hair strengthening and anti - hair loss composition comprises type XVII collagen, Platycladus orientalis leaf extract and copper acetopeptide; preferably, the mass ratio of type XVII collagen, Platycladus orientalis leaf extract and copper acetopeptide is (0.0001 - 0.1):(0.1 - 5):(0.001 - 0.5), for example, 1:100:10; Alternatively, the hair strengthening and anti - hair loss composition comprises type XVII collagen, Platycladus orientalis leaf extract and acetyl octapeptide - 2; preferably, the mass ratio of type XVII collagen, Platycladus orientalis leaf extract and acetyl octapeptide - 2 is (0.0001 - 0.1):(0.1 - 5):(0.001 - 0.5), for example, 1:100:

10.

9. A hair strengthening and anti - hair loss product, which comprises the hair strengthening and anti - hair loss composition according to any one of claims 1 - 8; Preferably, the hair strengthening and anti - hair loss product is shampoo, hair conditioner, hair essence, body wash or body lotion.

10. Use of the hair strengthening and anti - hair loss composition according to any one of claims 1 - 8 in the preparation of a hair strengthening and anti - hair loss product.

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