Gas positive pressure type solid phase extraction device and extraction method

Abstract

The invention discloses a gas positive pressure type solid-phase extraction device and an extraction method, and relates to the technical field of solid-phase extraction devices. A gas positive pressure type solid-phase extraction device comprises a base and further comprises a first supporting column and a second supporting column which are both rotationally installed on the base, an upper rotary disc and a lower rotary disc are longitudinally installed on the first supporting column in a sliding mode, a plurality of placing holes are formed in the upper rotary disc and the lower rotary disc, and the first supporting column and the second supporting column are arranged on the base. An upper bottle body and a lower bottle body are inserted into the two sets of containing holes respectively, an opening and closing part for driving the upper rotary disc and the lower rotary disc to ascend and descend is arranged on the first supporting column, and a switching part for driving the first supporting column and the second supporting column to rotate is arranged on the base. According to the device, multiple steps of extraction can be automatically realized, the device is more convenient, labor-saving and efficient to use, and liquid can pass through the filler more quickly, so that the solid-phase extraction efficiency is improved.

Description

Technical Field

[0001] The present invention belongs to the technical field of solid-phase extraction devices, and more specifically, relates to a gas positive-pressure type solid-phase extraction device. Background Art

[0002] Solid-phase extraction, abbreviated as SPE, is a sample pretreatment technology developed in recent years. It is developed from the combination of liquid-solid extraction and column liquid chromatography technology and is mainly used for sample separation, purification, and concentration. Existing solid-phase extraction devices are divided into negative-pressure type and positive-pressure type.

[0003] The positive-pressure type includes gas positive-pressure type and plunger rod positive-pressure type. In a gas positive-pressure type solid-phase extraction device, mainly through a gas pressurization system, pressure is applied to the upper end of the solid-phase extraction cartridge to form an upper and lower pressure difference for sample liquid extraction. To ensure the consistency of extraction, the gas positive-pressure type solid-phase extraction device must separately set a control valve for each cartridge, with a complex pressure control system and complex liquid addition, resulting in low extraction efficiency. In a plunger rod positive-pressure type solid-phase extraction device, mainly through the plunger rod and the cartridge cavity to form a piston cavity to push the sample liquid for extraction.

[0004] However, it is found in the use of the extraction device that in the processes of sample loading, rinsing, and elution, manual-assisted pausing operations are required, which will seriously affect the extraction efficiency. And during the extraction process, the extraction column with packing inside basically remains stationary, which will result in a slow flow rate of the medium and affect the extraction efficiency. Summary of the Invention

[0005] The technical problem to be solved by the present invention is to overcome the deficiencies of the prior art and provide a gas positive-pressure type solid-phase extraction device that can overcome or at least partially solve the above problems.

[0006] To solve the above technical problem, the basic concept of the technical solution adopted by the present invention is: A gas positive-pressure type solid-phase extraction device includes a base, and further includes: a first pillar and a second pillar, both rotatably installed on the base. Among them, an upper turntable and a lower turntable are longitudinally slidably installed on the first pillar. Multiple placement holes are provided on both the upper turntable and the lower turntable. An upper bottle body and a lower bottle body are respectively inserted into two groups of the placement holes. An opening and closing part for driving the upper turntable and the lower turntable to lift is provided on the first pillar. A switching part for driving the first pillar and the second pillar to rotate is provided on the base; a secondary turntable fixedly installed on the outer wall of the second pillar. Among them, multiple circumferentially distributed storage holes are provided on the secondary turntable, and extraction columns are provided in multiple of the storage holes. A driving part for driving the extraction columns to rotate is provided on the secondary turntable.

[0007] Preferably, the opening and closing part includes two groups of support plates fixedly connected to the first support column. Lifting devices are fixedly installed on both groups of support plates, and the telescopic ends of the two lifting devices are fixedly connected to the upper turntable and the lower turntable respectively.

[0008] Preferably, the switching part includes a first motor fixedly installed on the base. A first incomplete gear is fixedly installed on the output shaft of the first motor. A first gear that cooperates with the first incomplete gear is fixedly installed on the first support column. Among them, a second incomplete gear is fixedly installed on the first support column, and a second gear that cooperates with the second incomplete gear is fixedly installed on the second support column.

[0009] Preferably, the driving part includes a second motor fixedly installed on the auxiliary turntable. A rotating pipe is rotatably connected in the storage hole. A polygonal sleeve is fixedly connected to the outer wall of the extraction column, and the polygonal sleeve is inserted into the rotating pipe. Among them, a driven gear is fixedly installed on the outer wall of the rotating pipe, a ring gear that meshes with the driven gear is rotatably installed at the bottom of the auxiliary turntable, and a driving gear that meshes with the ring gear is fixedly installed on the output shaft of the second motor.

[0010] Furthermore, a plurality of cylinders are fixedly connected to the outer wall of the first support column. Exhaust holes and intake holes are respectively provided at both ends of the plurality of cylinders. A piston plate is longitudinally slidably installed in the cylinder, and a through hole is provided on the piston plate. A one-way valve is fixedly installed in the through hole. A pushing part for driving the piston plate to lift is provided on the base. The exhaust hole and the intake hole are respectively connected to the upper bottle body and the lower bottle body through connecting parts.

[0011] Furthermore, the pushing part includes a top rod fixedly connected to the lower end of the piston plate. The lower end of the top rod penetrates to the lower end of the cylinder. A convex platform is fixedly connected to the base. When the top rod moves to the position of the convex platform, the top rod drives the piston plate to slide upward, and a return spring is installed between the piston plate and the inner top of the cylinder.

[0012] Furthermore, the connecting part includes a first inner pipe arranged in the upper turntable and the lower turntable. Two second inner pipes are symmetrically arranged up and down in the first support column. Both groups of first inner pipes are respectively communicated with the two second inner pipes through hoses. Among them, the ends of the two second inner pipes are respectively connected to the exhaust hole and the intake hole. Annular plates are fixedly connected to the outer walls of the upper bottle body and the lower bottle body. An annular groove is provided at the lower end of the annular plate. A conveying hole extending into the upper bottle body and the lower bottle body is provided in the annular groove. The ends of the two first inner pipes respectively extend into the two annular grooves.

[0013] Furthermore, a plurality of top blocks arranged at equal intervals are fixedly connected to the lower end of the polygonal sleeve. A cross bar extending to the bottom of the polygonal sleeve is fixedly connected to the outer wall of the second support column.

[0014] Preferably, liquid inlet pipes and drain pipes are respectively arranged at the upper and lower ends of the extraction column. A feed pipe extending into one of the liquid inlet pipes is fixedly connected to the lower end of the upper bottle body. A feeding port is arranged at the top of the upper bottle body. A collecting pipe is fixedly connected to the top of the lower bottle body. The lower end of the drain pipe extends into one of the collecting pipes. The feed pipes of multiple upper bottle bodies and the collecting pipes of multiple lower bottle bodies are arranged vertically aligned with each other. Solenoid valves are fixedly installed in both the feed pipe and the collecting pipe.

[0015] A gas positive pressure type solid phase extraction method includes the following steps: S1. Load the sample solution, washing solution, and elution solution into the upper bottle body respectively; S2. Drive the upper turntable and the lower turntable to approach each other through a lifting device; S3. Open the solenoid valves in the feed pipe and the collecting pipe to allow the sample solution to enter the extraction column to complete sample loading; S4. Swap different upper bottle bodies and lower bottle bodies and sequentially complete the washing and elution operations; S5. During extraction, make the liquid passing through the extraction column generate a swirl and make the rotating extraction column vibrate; S6. During extraction, make a positive pressure generated at the inner top of the extraction column and a negative pressure generated at its inner bottom; S7. Take out the lower bottle body on the lower turntable.

[0016] After adopting the above technical solution, the present invention has the following beneficial effects compared with the prior art: 1. In the present invention, the first motor drives the first incomplete gear to rotate, and the upper turntable and the lower turntable can drive the upper bottle body and the lower bottle body to revolve, and rotate another upper bottle body and lower bottle body to be vertically aligned with the extraction column. At this time, the washing operation can be realized. When the first support rotates next time, the elution operation can be realized again, and each time a different lower bottle body is used for collection, so that multiple steps of extraction can be automatically realized, which is more convenient, labor-saving and efficient.

[0017] 2. In the present invention, when the first support rotates one week, the first support will also drive the second incomplete gear to rotate one week, and the auxiliary turntable will drive multiple extraction columns to revolve around the axis of the second support, so as to rotate another extraction column to the extraction station, that is, the position aligned with the upper bottle body and the lower bottle body, thus facilitating the automatic completion of another group of solid phase extraction operations.

[0018] 3. In the present invention, the second motor drives the annular gear to rotate, multiple driven gears will drive multiple rotating pipes to rotate, and the rotating pipes will drive the extraction column to rotate, so that the liquid passing through the extraction column can generate a swirl, enabling the liquid to pass through the packing faster, so as to improve the solid phase extraction efficiency.

[0019] 4. In the present invention, the extraction column drives the rotation of the polygonal sleeve on the outer wall, the polygonal sleeve drives the revolution of multiple top blocks around the axis of the extraction column, and the multiple top blocks will slide over the cross bar in sequence to generate vibrations, thereby causing the rotating extraction column to vibrate, which can further increase the flow rate of the liquid in the extraction column and improve the solid-phase extraction efficiency. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] In the drawings: Figure 1 is an isometric structural schematic diagram of a gas positive-pressure type solid-phase extraction device proposed by the present invention; Figure 2 is a partial isometric structural schematic diagram of a gas positive-pressure type solid-phase extraction device proposed by the present invention Figure 1 ; Figure 3 is a partial isometric structural schematic diagram of a gas positive-pressure type solid-phase extraction device proposed by the present invention Figure 2 ; Figure 4 is a partial isometric sectional structural schematic diagram of a gas positive-pressure type solid-phase extraction device proposed by the present invention; Figure 5 is a Figure 4 structural schematic diagram of part A of a gas positive-pressure type solid-phase extraction device proposed by the present invention; Figure 6 is an isometric structural schematic diagram of the auxiliary turntable of a gas positive-pressure type solid-phase extraction device proposed by the present invention; Figure 7 is a sectional isometric structural schematic diagram of the auxiliary turntable of a gas positive-pressure type solid-phase extraction device proposed by the present invention; Figure 8 is a sectional isometric structural schematic diagram of the cylinder block of a gas positive-pressure type solid-phase extraction device proposed by the present invention.

[0021] In the figure: 1, base; 2, first pillar; 3, upper turntable; 4, lower turntable; 5, second pillar; 6, auxiliary turntable; 7, extraction column; 8, liquid inlet pipe; 9, liquid discharge pipe; 10, storage hole; 11, placement hole; 12, upper bottle body; 13, lower bottle body; 14, feeding port; 15, collection pipe; 16, feed pipe; 17, limiting plate; 18, annular plate; 19, support plate; 20, lifting device; 21, first motor; 22, first incomplete gear; 23, first gear; 24, second incomplete gear; 25, second gear; 26, cylinder block; 27, exhaust hole; 28, air inlet hole; 29, piston plate; 30, return spring; 31, through hole; 32, ejector rod; 33, boss; 34, first inner tube; 35, annular groove; 36, conveying hole; 37, second inner tube; 38, hose; 39, annular gear; 40, driven gear; 41, second motor; 42, driving gear; 43, cross bar; 44, top block; 45, rotating tube; 46, polygonal sleeve. Specific embodiments

[0022] To make the objectives, technical solutions and advantages of the embodiments of the present invention clearer, the following will clearly and completely describe the technical solutions in the embodiments with reference to the accompanying drawings in the embodiments of the present invention. The following embodiments are used to illustrate the present invention, but are not used to limit the scope of the present invention.

[0023] Embodiment 1: Refer to Figures 1-8, A gas positive pressure solid phase extraction device, including a base 1 for supporting the entire device, and further including: a first pillar 2 and a second pillar 5 perpendicular to the base 1, both rotatably installed on the base 1. Among them, an upper turntable 3 and a lower turntable 4 are longitudinally slidably installed on the first pillar 2. A plurality of placement holes 11 are provided on both the upper turntable 3 and the lower turntable 4. An upper bottle body 12 and a lower bottle body 13 are respectively inserted into the two groups of placement holes 11. A plurality of upper bottle bodies 12 are respectively used for storing sample solutions, elution solutions, and elution solutions, etc. And the number of upper bottle bodies 12 is at least set to 3 - 6, and different numbers are required according to the target to be extracted. An opening and closing part for driving the upper turntable 3 and the lower turntable 4 to lift is provided on the first pillar 2. The opening and closing part includes two groups of support plates 19 fixedly connected to the first pillar 2. Lifting devices 20 are fixedly installed on both groups of support plates 19. The lifting devices 20 can be electric telescopic rods. The telescopic ends of the two groups of lifting devices 20 are respectively fixedly connected to the upper turntable 3 and the lower turntable 4. A switching part for driving the first pillar 2 and the second pillar 5 to rotate is provided on the base 1; a secondary turntable 6 fixedly installed on the outer wall of the second pillar 5. Among them, a plurality of circumferentially distributed storage holes 10 are provided on the secondary turntable 6. Extraction columns 7 are provided in a plurality of storage holes 10. Packing for adsorbing the target is provided in the extraction columns 7. A driving part for driving the extraction columns 7 to rotate is provided on the secondary turntable 6. Liquid inlet pipes 8 and drain pipes 9 are respectively provided at the upper and lower ends of the extraction column 7. The lower end of the upper bottle body 12 is fixedly connected to a feed pipe 16 extending into one of the liquid inlet pipes 8. A feeding port 14 is provided at the top of the upper bottle body 12. The top of the lower bottle body 13 is fixedly connected to a collection pipe 15. The lower end of the drain pipe 9 extends into one of the collection pipes 15. The feed pipes 16 of the plurality of upper bottle bodies 12 and the collection pipes 15 of the plurality of lower bottle bodies 13 are arranged vertically aligned. Solenoid valves are fixedly installed in both the feed pipe 16 and the collection pipe 15.

[0024] Specifically, the sample solution, the washing solution, and the elution solution are respectively filled into the upper bottle body 12. Then, the extraction column 7 with packing inside is respectively inserted into multiple transfer tubes 45 of the auxiliary turntable 6. Immediately afterwards, multiple upper bottle bodies 12 are successively inserted into the placement holes 11 of the upper turntable 3, and multiple lower bottle bodies 13 for storing waste liquid and extraction liquid are respectively inserted into multiple placement holes 11 of the lower turntable 4. And the feed pipe 16 of the upper bottle body 12 for storing the sample solution faces the liquid inlet pipe 8 of the extraction column 7, and the drain pipe 9 of the current extraction column 7 faces into the collection pipe 15 of one of the lower bottle bodies 13. Then, the lifting device 20 drives the upper turntable 3 and the lower turntable 4 to approach each other. Thus, the feed pipe 16 of one of the upper bottle bodies 12 is driven to be inserted into the liquid inlet pipe 8, and after the drain pipe 9 at the lower end of the current extraction column 7 is inserted into one of the collection pipes 15 below, the solenoid valves in the feed pipe 16 and the collection pipe 15 are opened. The sample solution in the upper bottle body 12 will enter the liquid inlet pipe 8 from the feed pipe 16, then enter the extraction column 7 from the liquid inlet pipe 8, and after being adsorbed by the packing, it is discharged from the drain pipe 9 into the collection pipe 15, and then enters the lower bottle body 13, thus realizing the sample loading operation. During this period, the switching part can automatically realize the switching of multiple upper bottle bodies 12, lower bottle bodies 13, and extraction columns 7, and can automatically realize multiple steps of extraction, which is more convenient, labor-saving and efficient to use. And the driving part can drive the extraction column 7 to rotate, so that the liquid passing through the extraction column 7 generates a swirl, enabling the liquid to pass through the packing faster, so as to improve the solid-phase extraction efficiency.

[0025] Example 2: Refer to Figure 2 , a gas positive pressure type solid-phase extraction device, which is basically the same as Example 1. Further: The switching part includes a first motor 21 fixedly installed on the base 1. The output shaft of the first motor 21 is fixedly installed with a first incomplete gear 22. A first gear 23 that cooperates with the first incomplete gear 22 is fixedly installed on the first support column 2. The teeth on the first incomplete gear 22 are incomplete. When the number of upper bottle bodies 12 is 4 groups, only one-fourth of the teeth on the first incomplete gear 22 are present. Among them, a second incomplete gear 24 is fixedly installed on the first support column 2, and a second gear 25 that cooperates with the second incomplete gear 24 is fixedly installed on the second support column 5. The second incomplete gear 24 is similar to the first incomplete gear 22.

[0026] Specifically, during extraction, the first motor 21 is started. The first motor 21 drives the first incomplete gear 22 to rotate. When the teeth on the first incomplete gear 22 mesh with the first gear 23, the first gear 23 starts to rotate. At this time, the electromagnetic valve is closed and the upper turntable 3 and the lower turntable 4 are separated from each other by the lifting device 20. The rotating first gear 23 drives the first support column 2 to rotate, and the first support column 2 drives the upper turntable 3 and the lower turntable 4 to rotate. The upper turntable 3 and the lower turntable 4 can drive the upper bottle body 12 and the lower bottle body 13 to revolve, and rotate another upper bottle body 12 and the lower bottle body 13 to be vertically aligned with the extraction column 7. At this time, the flushing work can be realized. When the first support column 2 rotates next time, the elution work can be realized, and each time a different lower bottle body 13 is used for collection, so that multiple steps of extraction can be automatically realized, which is more convenient, labor-saving and efficient.

[0027] When the first support column 2 rotates one week, the first support column 2 will also drive the second incomplete gear 24 to rotate one week. The second incomplete gear 24 meshes with the second gear 25 and drives it to rotate a fixed angle. The second gear 25 drives the auxiliary turntable 6 to rotate a fixed angle through the second support column 5. The auxiliary turntable 6 drives a plurality of extraction columns 7 to revolve around the axis of the second support column 5, so that another extraction column 7 can be rotated to the extraction station, that is, the position aligned with the upper bottle body 12 and the lower bottle body 13, so as to facilitate the automatic completion of another set of solid-phase extraction work.

[0028] Example 3: Refer to Figures 6-7 , a gas positive pressure type solid-phase extraction device, which is basically the same as Example 2. Further: The driving part includes a second motor 41 fixedly installed on the auxiliary turntable 6. A rotating pipe 45 is rotatably connected in the storage hole 10. A polygonal sleeve 46 is fixedly connected to the outer wall of the extraction column 7. The polygonal sleeve 46 is inserted into the rotating pipe 45. Among them, a driven gear 40 is fixedly installed on the outer wall of the rotating pipe 45. A ring gear 39 meshing with the driven gear 40 is rotatably installed at the bottom of the auxiliary turntable 6. The output shaft of the second motor 41 is fixedly installed with a driving gear 42 meshing with the ring gear 39. A limiting plate 17 that rests on the upper port of the rotating pipe 45 is also fixedly connected to the upper end outer wall of the extraction column 7.

[0029] Specifically, during extraction, the second motor 41 is started. The second motor 41 drives the ring gear 39 to rotate through the driving gear 42. The ring gear 39 drives a plurality of driven gears 40 to rotate. The plurality of driven gears 40 drive a plurality of rotating pipes 45 to rotate. The rotating pipes 45 drive the extraction columns 7 to rotate. Thus, a swirling flow can be generated in the liquid passing through the extraction column 7, so that the liquid can pass through the packing faster, so as to improve the solid-phase extraction efficiency.

[0030] A lower end of the above-mentioned polygonal sleeve 46 is fixedly connected with a plurality of top blocks 44 arranged at equal intervals, and an outer wall of the second support column 5 is fixedly connected with a cross bar 43 extending to the bottom of the polygonal sleeve 46.

[0031] Specifically, when the extraction column 7 rotates, the extraction column 7 drives the polygonal sleeve 46 on the outer wall to rotate, and the polygonal sleeve 46 drives the plurality of top blocks 44 to revolve around the axis of the extraction column 7. The plurality of top blocks 44 will sequentially slide over the cross bar 43 to generate vibrations, so that the rotating extraction column 7 generates vibrations, thereby further increasing the flow rate of the liquid in the extraction column 7 and improving the solid-phase extraction efficiency.

[0032] Example 4: Refer to Figures 3-5 and Figure 8 , a gas positive pressure type solid-phase extraction device, which is basically the same as that in Example 3. Further, a plurality of cylinders 26 are fixedly connected to an outer wall of the first support column 2. The number of the cylinders 26 is the same as the number of the upper bottle bodies 12. Exhaust holes 27 and air inlet holes 28 are respectively arranged at two ends of the plurality of cylinders 26. A piston plate 29 is longitudinally slidably installed in the cylinder 26, and a through hole 31 is arranged on the piston plate 29. A one-way valve is fixedly installed in the through hole 31. A pushing part for driving the piston plate 29 to lift is arranged on the base 1. The exhaust hole 27 and the air inlet hole 28 are respectively connected to the upper bottle body 12 and the lower bottle body 13 through connecting parts. The pushing part includes a top rod 32 fixedly connected to a lower end of the piston plate 29. A lower end of the top rod 32 penetrates to the lower end of the cylinder 26. A convex platform 33 is fixedly connected to the base 1. When the top rod 32 moves to the position of the convex platform 33, the top rod 32 drives the piston plate 29 to slide upward. A return spring 30 is installed between the piston plate 29 and an inner top of the cylinder 26; the connecting part includes a first inner tube 34 arranged in the upper turntable 3 and the lower turntable 4. Two second inner tubes 37 are symmetrically arranged up and down in the first support column 2. Two groups of the first inner tubes 34 are respectively communicated with two groups of the second inner tubes 37 through hoses 38. Among them, ends of the two second inner tubes 37 are respectively connected to the exhaust hole 27 and the air inlet hole 28. Annular plates 18 are fixedly connected to outer walls of the upper bottle body 12 and the lower bottle body 13. An annular groove 35 is arranged at a lower end of the annular plate 18. A conveying hole 36 extending into the upper bottle body 12 and the lower bottle body 13 is arranged in the annular groove 35. Ends of the two first inner tubes 34 respectively extend into the two groups of annular grooves 35.

[0033] Specifically, during the rotation of the first support column 2, the first support column 2 also drives a plurality of cylinders 26 to revolve around their axes. When the cylinder 26 moves to the extraction station, the ejector rod 32 at the bottom presses against the boss 33. Under the action of the boss 33, the ejector rod 32 drives the piston plate 29 to slide towards the inner top of the cylinder 26. The air inside the inner top of the cylinder 26 will be compressed, the air pressure will increase, and it will be transported to the annular groove 35 through the exhaust hole 27, the second inner tube 37, the hose 38, and the first inner tube 34, and then transported into the upper bottle body 12 through the delivery hole 36. The air pressure inside the upper bottle body 12 will increase. When the solenoid valve inside the feed pipe 16 is opened, the positive pressure inside the upper bottle body 12 will enable the internal liquid to flow more efficiently into the extraction column 7, so as to improve the extraction efficiency. A negative pressure will be generated inside the inner bottom of the cylinder 26, and a negative pressure will be generated inside the lower bottle body 13. When the solenoid valve inside the collection pipe 15 is opened, the lower bottle body 13 will suck air from the drain pipe 9 of the extraction column 7, enabling a positive pressure to be generated at the inner top of the extraction column 7 and a negative pressure to be generated at its inner bottom, significantly improving the solid-phase extraction efficiency. When the cylinder 26 disengages from the extraction station, the boss 33 no longer presses against the ejector rod 32, and the return spring 30 drives the piston plate 29 and the ejector rod 32 to move downward and reset. At this time, due to the setting of the through hole 31 and a one-way valve being installed inside the through hole 31, the downward moving piston plate 29 will not cause an air pressure difference inside the cylinder 26. That is to say, the gas at the inner bottom of the cylinder 26 can flow towards its inner top through the through hole 31 at this time.

[0034] Example 5: Refer to Figures 1-8 , a gas positive-pressure type solid-phase extraction method, comprising the following steps: S1. Respectively load the sample solution, the washing solution, and the elution solution into the upper bottle body 12; S2. Drive the upper turntable 3 and the lower turntable 4 to approach each other through the lifting device 20; S3. Open the solenoid valves inside the feed pipe 16 and the collection pipe 15 to enable the sample solution to enter the extraction column 7 to complete sample loading; S4. Exchange different upper bottle bodies 12 and lower bottle bodies 13, and sequentially complete the washing and elution operations; S5. During extraction, make the liquid passing through the extraction column 7 generate a swirl flow, and make the rotating extraction column 7 vibrate; S6. During extraction, make a positive pressure be generated at the inner top of the extraction column 7, and a negative pressure be generated at its inner bottom; S7. Take out the lower bottle body 13 on the lower turntable 4.

[0035] In the use of the present invention, a sample solution, a washing solution, and an elution solution are respectively filled into the upper bottle body 12. Then, extraction columns 7 with packing inside are respectively inserted into a plurality of rotating tubes 45 of the auxiliary turntable 6. Immediately afterwards, a plurality of upper bottle bodies 12 are successively inserted into the placement holes 11 of the upper turntable 3, and a plurality of lower bottle bodies 13 for storing waste liquid and extraction liquid are respectively inserted into a plurality of placement holes 11 of the lower turntable 4. And the feed pipe 16 of the upper bottle body 12 for storing the sample solution faces the liquid inlet pipe 8 of the extraction column 7, and the drain pipe 9 of the current extraction column 7 faces the collection pipe 15 of one of the lower bottle bodies 13. Then, the lifting device 20 drives the upper turntable 3 and the lower turntable 4 to approach each other. As a result, the feed pipe 16 of one of the upper bottle bodies 12 is driven to be inserted into the liquid inlet pipe 8, and the drain pipe 9 at the lower end of the current extraction column 7 is inserted into one of the lower collection pipes 15 below. After that, the solenoid valves in the feed pipe 16 and the collection pipe 15 are opened, and the sample solution in the upper bottle body 12 will enter the liquid inlet pipe 8 from the feed pipe 16, then enter the extraction column 7 from the liquid inlet pipe 8, and after being adsorbed by the packing, it is discharged from the drain pipe 9 into the collection pipe 15, and then enters the lower bottle body 13, thus realizing the sample loading operation. During this period, the first motor 21 is started, and the first motor 21 drives the first incomplete gear 22 to rotate. When the teeth on the first incomplete gear 22 mesh with the first gear 23, the first gear 23 starts to rotate. At this time, the solenoid valve is closed and the lifting device 20 drives the upper turntable 3 and the lower turntable 4 to separate from each other. And the rotating first gear 23 drives the first support column 2 to rotate, and the first support column 2 drives the upper turntable 3 and the lower turntable 4 to rotate. The upper turntable 3 and the lower turntable 4 can drive the upper bottle body 12 and the lower bottle body 13 to revolve, and make another upper bottle body 12 and the lower bottle body 13 rotate to be vertically aligned with the extraction column 7. At this time, the washing operation can be realized. When the first support column 2 rotates next time, the elution operation can be realized, and each time a different lower bottle body 13 is used for collection, so that multiple steps of extraction can be automatically realized, and the use is more convenient, labor-saving and efficient.

[0036] When the first support column 2 rotates one week, the first support column 2 will also drive the second incomplete gear 24 to rotate one week. The second incomplete gear 24 meshes with the second gear 25 and drives it to rotate a fixed angle, and the second gear 25 drives the auxiliary turntable 6 to rotate a fixed angle through the second support column 5. The auxiliary turntable 6 drives a plurality of extraction columns 7 to revolve around the axis of the second support column 5, so that another extraction column 7 can be rotated to the extraction station, that is, the position aligned with the upper bottle body 12 and the lower bottle body 13, thus facilitating the automatic completion of another set of solid phase extraction work.

[0037] During extraction, the second motor 41 is started. The second motor 41 drives the ring gear 39 to rotate through the driving gear 42. The ring gear 39 drives a plurality of driven gears 40 to rotate. The plurality of driven gears 40 drive a plurality of rotating tubes 45 to rotate. The rotating tubes 45 drive the extraction column 7 to rotate. Thus, a swirl can be generated in the liquid passing through the extraction column 7, enabling the liquid to pass through the packing faster, so as to improve the solid-phase extraction efficiency.

[0038] The above are only the preferred embodiments of the present invention, and do not impose any form of limitation on the present invention. Although the present invention has been disclosed above with the preferred embodiments, it is not intended to limit the present invention. Any person skilled in the art of the present invention can make some changes or modifications to equivalent embodiments by using the technical content prompted above within the scope of the technical solution of the present invention. However, as long as it does not depart from the content of the technical solution of the present invention, any simple modification, equivalent change and modification made to the above embodiments according to the technical essence of the present invention still fall within the scope of the present invention.

Claims

1. A gas positive pressure type solid phase extraction device, comprising a base (1), characterized in that, Further comprising: A first pillar (2) and a second pillar (5), both rotatably mounted on the base (1), wherein, an upper turntable (3) and a lower turntable (4) are longitudinally slidably mounted on the first pillar (2), a plurality of placement holes (11) are provided on both the upper turntable (3) and the lower turntable (4), an upper bottle body (12) and a lower bottle body (13) are respectively inserted into two groups of the placement holes (11), an opening and closing part for driving the upper turntable (3) and the lower turntable (4) to lift is provided on the first pillar (2), and a switching part for driving the first pillar (2) and the second pillar (5) to rotate is provided on the base (1); A secondary turntable (6) fixedly mounted on the outer wall of the second pillar (5), wherein, a plurality of circumferentially distributed storage holes (10) are provided on the secondary turntable (6), extraction columns (7) are provided in a plurality of the storage holes (10), and a driving part for driving the extraction columns (7) to rotate is provided on the secondary turntable (6).

2. The gas positive pressure type solid phase extraction device according to claim 1, characterized in that, The opening and closing part includes two groups of support plates (19) fixedly connected to the first pillar (2), lifting devices (20) are fixedly mounted on both of the two groups of support plates (19), and the telescopic ends of the two groups of lifting devices (20) are respectively fixedly connected to the upper turntable (3) and the lower turntable (4).

3. The gas positive pressure type solid phase extraction device according to claim 1, characterized in that, The switching part includes a first motor (21) fixedly mounted on the base (1), a first incomplete gear (22) is fixedly mounted on the output shaft of the first motor (21), and a first gear (23) cooperating with the first incomplete gear (22) is fixedly mounted on the first pillar (2), wherein, a second incomplete gear (24) is fixedly mounted on the first pillar (2), and a second gear (25) cooperating with the second incomplete gear (24) is fixedly mounted on the second pillar (5).

4. A positive pressure gas solid phase extraction device according to claim 1, characterized in that, The driving part includes a second motor (41) fixedly mounted on the secondary turntable (6), a rotating pipe (45) is rotatably connected in the storage hole (10), a polygonal sleeve (46) is fixedly connected to the outer wall of the extraction column (7), and the polygonal sleeve (46) is inserted into the rotating pipe (45), wherein, a driven gear (40) is fixedly mounted on the outer wall of the rotating pipe (45), an annular gear (39) meshing with the driven gear (40) is rotatably mounted at the bottom of the secondary turntable (6), and a driving gear (42) meshing with the annular gear (39) is fixedly mounted on the output shaft of the second motor (41).

5. A positive pressure gas solid phase extraction device according to claim 4, characterized in that, A plurality of cylinders (26) are fixedly connected to the outer wall of the first pillar (2), exhaust holes (27) and air inlet holes (28) are respectively provided at both ends of the plurality of cylinders (26), a piston plate (29) is longitudinally slidably mounted in the cylinder (26), and a through hole (31) is provided on the piston plate (29), a one-way valve is fixedly mounted in the through hole (31), a pushing part for driving the piston plate (29) to lift is provided on the base (1), and the exhaust holes (27) and the air inlet holes (28) are respectively connected to the upper bottle body (12) and the lower bottle body (13) through connecting parts.

6. The gas positive pressure type solid phase extraction device according to claim 5, characterized in that, The pushing part includes a push rod (32) fixedly connected to the lower end of the piston plate (29). The lower end of the push rod (32) penetrates to the lower end of the cylinder block (26). A boss (33) is fixedly connected to the base (1). When the push rod (32) moves to the position of the boss (33), the push rod (32) drives the piston plate (29) to slide upward. A return spring (30) is installed between the piston plate (29) and the inner top of the cylinder block (26).

7. The gas positive pressure type solid phase extraction device according to claim 5, characterized in that, The connecting part includes a first inner tube (34) arranged in the upper turntable (3) and the lower turntable (4). Two second inner tubes (37) are symmetrically arranged up and down in the first pillar (2). Both groups of the first inner tubes (34) are respectively connected to the two groups of second inner tubes (37) through hoses (38). Among them, the ends of the two second inner tubes (37) are respectively connected to the exhaust hole (27) and the intake hole (28). Ring plates (18) are fixedly connected to the outer walls of the upper bottle body (12) and the lower bottle body (13). An annular groove (35) is provided at the lower end of the ring plate (18). A delivery hole (36) extending into the upper bottle body (12) and the lower bottle body (13) is provided in the annular groove (35). The ends of the two first inner tubes (34) respectively extend into the two groups of annular grooves (35).

8. The gas positive pressure type solid phase extraction device according to claim 4, characterized in that, A plurality of top blocks (44) arranged at equal intervals are fixedly connected to the lower end of the polygonal sleeve (46). A cross bar (43) extending to the bottom of the polygonal sleeve (46) is fixedly connected to the outer wall of the second pillar (5).

9. The gas positive pressure type solid phase extraction device according to claim 1, wherein Liquid inlet pipes (8) and liquid discharge pipes (9) are respectively arranged at the upper and lower ends of the extraction column (7). A feed pipe (16) extending into one of the liquid inlet pipes (8) is fixedly connected to the lower end of the upper bottle body (12). A feeding port (14) is provided at the top of the upper bottle body (12). A collecting pipe (15) is fixedly connected to the top of the lower bottle body (13). The lower end of the liquid discharge pipe (9) extends into one of the collecting pipes (15). The feed pipes (16) of the plurality of upper bottle bodies (12) and the collecting pipes (15) of the plurality of lower bottle bodies (13) are arranged vertically aligned. Solenoid valves are fixedly installed in both the feed pipe (16) and the collecting pipe (15).

10. A positive pressure gas solid phase extraction method, characterized in that, Using a gas positive pressure type solid phase extraction device as described in claim 7, the following steps are included: S1. Respectively load the sample solution, the washing solution, and the elution solution into the upper bottle body (12). S2. Drive the upper turntable (3) and the lower turntable (4) to approach each other through the lifting device (20). S3. Open the solenoid valves in the feed pipe (16) and the collecting pipe (15) to enable the sample solution to enter the extraction column (7) to complete sample loading. S4. Exchange different upper bottle bodies (12) and lower bottle bodies (13) and sequentially complete the washing and elution operations. S5. During extraction, make the liquid passing through the extraction column (7) generate a swirl and make the rotating extraction column (7) vibrate. S6. During extraction, make a positive pressure generated at the inner top of the extraction column (7), while a negative pressure is generated at its inner bottom. S7. Take out the lower bottle body (13) on the lower turntable (4).

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