Plant cell culture fluid mixing device

Abstract

The invention relates to the technical field of cell culture fluid preparation, and particularly discloses a plant cell culture fluid mixing device. Comprising a mixing cavity, a top cover detachably connected with the mixing cavity, a motor arranged on the top cover, a stirring shaft coaxially connected with an output shaft of the motor and located in the mixing cavity, a scattering assembly connected with the stirring shaft and used for scattering raw materials firstly, and a stirring assembly used for mixing the raw materials later; the weighing assembly rotates on the basis of rotation of the stirring shaft and is used for enabling raw materials to fall into the mixing cavity under rotation, a discharging assembly is arranged on the weighing assembly, and the discharging assembly is opened on the basis of rotation of the weighing assembly; the device solves the problem that the quality of the mixed plant culture solution is unstable due to the mixing error of the traditional plant culture solution.

Description

Technical Field

[0001] This application relates to the technical field of cell culture medium preparation, and specifically discloses a mixing device for plant cell culture media. Background Art

[0002] A culture medium is a type of medium, and a liquid medium is called a culture medium. Media are divided into liquid media, solid media, and semi-solid media; The process of preparing a culture medium involves multiple steps, including selecting appropriate nutrients, adjusting the pH value, and performing sterilization treatment. During the preparation process, appropriate nutrients are selected according to the characteristics and nutritional requirements of the cells to be cultured. After selecting the appropriate nutrients, the selected substances need to be weighed by personnel and then mixed into a tank for stirring. After adjusting the pH value, finally, an equal amount is poured into cell culture tubes as required; During the mixing process of various substances, the following problems exist: In the mixing of culture media with viscous properties, such as MS medium, which consists of high-concentration inorganic salts, sucrose, and agar. Agar is used as a solidifying agent, sucrose provides a carbon source and increases the osmotic pressure, and the high concentration of inorganic salts makes the solution viscous. It is widely used in the culture of organs, anthers, cells, and protoplasts, such as tobacco, rice, etc.; Focusing on this important component of agar, the medium with added agar forms a viscous liquid after melting, but usually, it is heated and dissolved during preparation and solidifies after cooling. At this time, problems with errors will occur: the weighed agar is weighed as a solid, then heated and poured in, and this exchange process will cause errors in the quality of agar; After obtaining the masses of various substances through a measuring cup or a weighing device, a transfer device is often required to transfer them into the mixing device for plant cell culture media. In this process, there are errors. In view of this, the present invention provides a mixing device for plant cell culture media to solve the above problems. Summary of the Invention

[0003] The purpose of the present invention is to solve the problem that traditional plant culture media have mixing errors, which in turn leads to unstable quality of the mixed plant culture media.

[0004] To achieve the above purpose, the present invention provides the following basic solution: A mixing device for plant cell culture media, comprising a mixing chamber, a top cover detachably connected to the mixing chamber, a motor provided on the top cover, a stirring shaft coaxially connected to the output shaft of the motor and located inside the mixing chamber, a dispersing assembly connected to the stirring shaft for first dispersing the raw materials, and a stirring assembly for subsequent mixing; It further includes a weighing assembly that rotates on its own axis based on the rotation of the stirring shaft and is used for the raw materials to rotate and fall into the mixing chamber. An unloading assembly is arranged on the weighing assembly, and the unloading assembly is opened based on the rotation of the weighing assembly; The weighing assembly includes a plurality of discharge pipes, auxiliary holes opened in the top cover that match the positions and quantities of the discharge pipes, second rotating bearings installed in the auxiliary holes for connecting the discharge pipes, connecting pipes detachably connected to one end of the discharge pipes, and a weighing cylinder connected to the upper end of the unloading assembly. A cover plate is detachably connected to the top of the weighing cylinder, and the other end of the connecting pipe is connected to the lower end of the unloading assembly; The connecting pipe is threadedly connected to the discharge pipe. It further includes a driven gear, and an installation hole is opened in the driven gear. The connecting pipe passes through the installation hole and is fixedly connected to the driven gear. The weighing cylinder is transparent and a scale is engraved on the outer wall of the weighing cylinder. The end of the discharge pipe away from the weighing cylinder is located in the mixing chamber and above the dispersing assembly; The second rotating bearing is installed in the auxiliary hole. A main hole is opened in the center of the top cover, and a first rotating bearing is installed in the main hole. The inner ring of the first rotating bearing is used to connect the outer wall of the stirring shaft. The part of the stirring shaft located outside the mixing chamber is coaxially connected with a driving gear, and the driving gear meshes with the driven gear; The unloading assembly includes a cylinder body, a plurality of hollow partition plates circumferentially and uniformly fixed inside the cylinder body, leakage holes opened in the hollow partition plates, a plugging plate that can move inside the hollow partition plates, and a magnet sheet arranged inside the hollow partition plates for magnetically attracting the plugging plate. When the unloading assembly rotates to generate centrifugal force, the centrifugal force overcomes the magnetic force to change the position of the plugging plate and expose the leakage holes, and the leakage holes are at the lowest point position of the hollow partition plates.

[0005] Further, the dispersing assembly is arranged based on the stirring shaft. The dispersing assembly includes a frame symmetrically arranged on the outer periphery of the stirring shaft and a plurality of steel wires evenly arranged inside the frame. The steel wires are arranged vertically, and the gaps between adjacent steel wires are the same.

[0006] Further, the stirring assembly is arranged based on the stirring shaft and is arranged below the dispersing assembly. The stirring assembly includes a horizontal stirring rod and an inclined stirring rod. Both the horizontal stirring rod and the inclined stirring rod are connected to the stirring shaft, and the horizontal stirring rod is located below the inclined stirring rod. The inclined stirring rods are all inclined upward.

[0007] Further, the mixing chamber and the top cover are made of the same material, both are made of stainless steel. An external thread is arranged on the outer top of the mixing chamber, and an internal thread is arranged on the inner wall of the top cover. The mixing chamber and the top cover are connected through the external thread and the internal thread.

[0008] Further, a circular groove is formed at the top of the top cover, a magnet is arranged in the circular groove, and a protective shell magnetically connected to the magnet is further included for protecting the driving gear and the driven gear and restricting the violent shaking of the weighing cylinder.

[0009] Further, the protective shell includes a shell body, an iron sheet arranged at the bottom of the shell body for magnetically connecting with the magnet, a protective hole and a motor protection hole formed at the top of the shell body. The motor is installed through the motor protection hole, and the weighing cylinder is installed through the protective hole.

[0010] The principle and effect of this solution are as follows: 1. Compared with the prior art, the structure of this device is simple and ingenious. The core lies in that the weighing device is highly integrated on this mixing device, solving the problem that after obtaining the masses of various substances through a measuring cup or a weighing device, it is often necessary to use transition equipment to transfer them to the plant cell culture medium mixing device, and there are errors in this process.

[0011] 2. Compared with the prior art, this invention is provided with multiple discharge pipes based on the weighing device, and discharge assemblies are arranged on the discharge pipes. Before the mixing action starts, no material will be discharged, and all raw materials stay in the weighing device, facilitating relevant staff to observe whether the weighing is accurate. Therefore, this device can achieve precise mixing.

[0012] 3. Compared with the prior art, this device has advantages in discharging. Traditionally, it is natural discharging based on gravity, while this device uses rotational discharging based on gravity. This kind of discharging is faster and is not prone to the problem that raw materials stay in the weighing device and cannot flow out, and it is suitable for the mixing of viscous culture media.

[0013] 4. Compared with the prior art, this device is provided with a stirring assembly. The stirring assembly drives the weighing device to rotate, thereby realizing the rotational discharging of the weighing device. At the same time, when driving the weighing device to rotate and discharge, the discharge assembly is opened, that is, the discharging can only be realized when the mixing action starts, which is beneficial to achieving precise mixing of raw materials.

[0014] 5. Compared with the prior art, the discharge assembly is compared with the traditional valve body structure. Although the traditional valve body structure can also realize the retention of raw materials, there is also the problem that the raw materials do not fall cleanly, especially more obvious in viscous raw materials. The discharge assembly in this device can not only realize the retention of raw materials, but is better than the traditional valve body structure in the problem of the raw materials not falling cleanly, and the problem of the raw materials not falling cleanly is not obvious.

[0015] 6. Compared with the prior art, the present device has made improvements in stirring. Traditional stirring is the mixing of substances with substances. In order to improve the mixing degree, the present device is provided with a dispersing component, which is integrated on the stirring component, so that the raw materials first come into contact with the dispersing component. The dispersing component disperses the raw materials from a large volume to a small volume, and then the raw materials in a small volume will come into contact with the stirring component. The raw materials in a small volume have certain advantages in stirring and mixing, and the mixing degree and mixing efficiency are better than those of traditional stirring and mixing. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] In order to more clearly illustrate the technical solutions in the embodiments of the present application, the following will briefly introduce the drawings required for the description of the embodiments. Obviously, the drawings in the following description are only some embodiments of the present application. For those skilled in the art, without creative efforts, other drawings can also be obtained based on these drawings.

[0017] Figure 1 FIG. shows an exploded schematic view of a plant cell culture medium mixing device proposed in an embodiment of the present application; Figure 2 FIG. shows a complete front view schematic diagram of the installation of a plant cell culture medium mixing device proposed in an embodiment of the present application; Figure 3 FIG. shows a plant cell culture medium mixing device proposed in an embodiment of the present application Figure 1 specific structural schematic diagram of part A; Figure 4 FIG. shows a structural schematic diagram of a mixing component in a plant cell culture medium mixing device proposed in an embodiment of the present application; Figure 5 FIG. shows a structural schematic diagram of a protective shell in a plant cell culture medium mixing device proposed in an embodiment of the present application; Figure 6 FIG. shows a plant cell culture medium mixing device proposed in an embodiment of the present application Figure 3 cross-sectional schematic diagram of the hollow partition board therein. DETAILED DESCRIPTION OF THE EMBODIMENTS

[0018] In order to further elaborate on the technical means and effects adopted by the present invention to achieve the predetermined invention purpose, the following will, in conjunction with the accompanying drawings and preferred embodiments, describe in detail the specific implementation manners, structures, features and their effects of the present invention as follows.

[0019] The reference numerals in the accompanying drawings of the specification include: mixing chamber 1, external thread 2, stirring shaft 3, top cover 4, circular groove 5, secondary hole 6, motor 7, first rotating bearing 8, driving gear 9, discharge pipe 10, second rotating bearing 11, connecting pipe 12, driven gear 13, weighing cylinder 14, cover plate 15, cylinder body 16, hollow partition plate 17, leakage hole 18, plugging plate 19, magnet sheet 20, frame 21, steel wire 22, inclined stirring rod 23, horizontal stirring rod 24, protective shell 25, motor protection hole 26, protection hole 27.

[0020] For example, in the embodiment Figures 1-6 as shown: A plant cell culture medium mixing device includes a mixing chamber 1, a top cover 4 detachably connected to the mixing chamber 1, a motor 7 provided on the top cover 4, and a stirring shaft 3 coaxially connected to the output shaft of the motor 7 and located inside the mixing chamber 1, a dispersing assembly connected to the stirring shaft 3 for first dispersing raw materials and a stirring assembly for subsequent mixing; it further includes a weighing assembly that rotates self - rotatably based on the rotation of the stirring shaft 3 for the raw materials to rotate and fall into the mixing chamber 1, and a discharge assembly is provided on the weighing assembly, and the discharge assembly opens based on the rotation of the weighing assembly.

[0021] Regarding the material selection of the mixing chamber 1 and the top cover 4: The materials of the mixing chamber 1 and the top cover 4 are the same, both are made of 304 stainless steel. Regarding the connection between the mixing chamber 1 and the top cover 4: An external thread 2 is provided on the outer top of the mixing chamber 1, and an internal thread is provided on the inner wall of the top cover 4. The mixing chamber 1 and the top cover 4 are connected through the external thread 2 and the internal thread.

[0022] Through the threaded connection of the mixing chamber 1 and the top cover 4, the rapid opening and closing of the mixing chamber 1 and the top cover 4 are realized, and then the mixed plant cell culture medium in the mixing chamber 1 is poured out, realizing the rapid collection of the mixed plant cell culture medium. Such as Figure 1 and Figure 2 as shown: The weighing assembly includes a plurality of discharge pipes 10, secondary holes 6 opened on the top cover 4 that match the positions and quantities of the discharge pipes 10, second rotating bearings 11 installed in the secondary holes 6 for connecting the discharge pipes 10, a connecting pipe 12 detachably connected to one end of the discharge pipe 10, and a weighing cylinder 14 connected to the connecting pipe 12. A cover plate 15 is detachably connected to the top of the weighing cylinder 14, and the discharge assembly is provided between the weighing cylinder 14 and the connecting pipe 12.

[0023] Specifically: Based on most existing plant cell culture media, four discharge pipes 10 are selected in this case. The discharge pipes 10 are all installed in the secondary holes 6. At the same time, a second rotating bearing 11 needs to be installed in the secondary holes 6 to avoid the movement interference caused by the self-rotation of the discharge pipes 10. The top of the discharge pipe 10 is used to connect the connecting pipe 12. The connecting pipe 12 is threadedly connected to the discharge pipe 10. The top of the connecting pipe 12 is connected to the weighing cylinder 14. The weighing cylinder 14 is the metering device in this case and uses volume metering. Therefore, for the observation of relevant staff, the weighing cylinder 14 is transparent and the outer wall of the weighing cylinder 14 is engraved with a scale. The top of the weighing cylinder 14 is threadedly connected with a cover plate 15. Since the weighing cylinder 14 will rotate, to avoid the splashing of raw materials, a cover plate 15 is provided to avoid the splashing phenomenon of raw materials; As Figure 1 and Figure 2 shown: It also includes a driven gear 13. An installation hole is opened on the driven gear 13. The connecting pipe 12 passes through the installation hole and is fixedly connected to the driven gear 13. After the connecting pipe 12 passes through the installation hole and is fixedly connected to the driven gear 13, the raw materials in the weighing cylinder 14 can enter the discharge assembly, then enter the connecting pipe 12, and finally reach the inside of the mixing chamber 1 through the connecting pipe 12; Regarding the self-rotation of the weighing cylinder 14: Since the second rotating bearing 11 has been installed in the secondary hole 6 to avoid the movement interference of the self-rotation of the discharge pipe 10, a main hole is opened at the center of the top cover 4. A first rotating bearing 8 is installed in the main hole. The inner ring of the first rotating bearing 8 is used to connect the outer wall of the stirring shaft 3. The first rotating bearing 8 avoids the movement interference of the connecting shaft. The part of the stirring shaft 3 located outside the mixing chamber 1 is coaxially connected to the driving gear 9. The driving gear 9 meshes with the driven gear 13.

[0024] Specifically: After the motor 7 is started, it drives the stirring shaft 3 to rotate self, realizing the rotation of the driving gear 9. When the driving gear 9 rotates, since the driving gear 9 meshes with the driven gear 13, the driven gear 13 rotates, and then the discharge pipe 10 rotates. After the discharge pipe 10 rotates, the weighing cylinder 14 and the discharge assembly rotate self; For the weighing cylinder 14: Due to the characteristics of the plant culture medium, the raw materials in the weighing cylinder 14 include non-viscous plant culture medium raw materials and viscous plant culture medium raw materials. Therefore, the rotating weighing cylinder 14 can accelerate the falling of the viscous plant culture medium raw materials, solve the phenomenon that the viscous plant culture medium raw materials stay in the weighing cylinder 14, and thus ensure the accuracy of the raw materials; Since most of the raw materials for viscous plant culture media use agar, the culture medium with agar forms a viscous liquid after melting. However, it is usually heated and dissolved during preparation and solidifies after cooling. Based on this, in this case, the measuring cylinder 14 is further improved. An electric heating resistance wire needs to be installed on the inner wall of the measuring cylinder 14 to ensure that when mixing certain raw materials that require agar as the raw material for the viscous plant culture medium, the agar is in a liquid state, which can accelerate the subsequent mixing of raw materials and prevent the agar from solidifying; Regarding the discharging assembly, the discharging assembly is the core of this case: As Figure 3 shown: The discharging assembly includes a cylinder body 16, a number of hollow partition plates 17 fixedly connected to the inside of the cylinder body 16 circumferentially and uniformly, leakage holes 18 opened on the hollow partition plates 17, a plugging plate 19 that can move inside the hollow partition plates 17, and a magnet sheet 20 arranged on the inner side of the hollow partition plates 17 for magnetically attracting the plugging plate 19. When the discharging assembly rotates to generate centrifugal force, the centrifugal force overcomes the magnetic force to change the position of the plugging plate 19 to expose the leakage holes 18, and the leakage holes 18 are located at the lowest point of the hollow partition plates 17.

[0025] When the measuring cylinder 14 does not rotate, it means that the motor 7 has not been started and the mixing has not begun. Then, for the measuring cylinder 14, enough time can be given to the operator for precise weighing. At this time, for the discharging assembly, the plugging plate 19 is in a connected state with the magnet sheet 20 due to magnetic attraction, the leakage holes 18 are blocked, and the liquid in the measuring cylinder 14 does not drop, which exactly fits the situation of giving the operator enough time for precise weighing. When the motor 7 is started, based on the above-mentioned setting relationship between the driving gear 9 and the driven gear 13, the measuring cylinder 14 rotates, and the discharging assembly also rotates. The rotating cylinder body 16 will inevitably generate centrifugal force, and the centrifugal force causes centrifugal movement. When the centrifugal force is greater than the magnetic force brought by the above-mentioned magnet sheet 20, the plugging plate 19 starts to move. Since there is a moving space for the plugging plate 19 inside the hollow partition plates 17 and the moving position is centrifugal, the leakage holes 18 are exposed. The raw materials in the measuring cylinder 14 rotate and fall due to gravity and start to enter the discharging pipe 10 through the leakage holes 18, and finally reach the mixing chamber 1; Specifically: As Figure 6 shown, because the leakage holes 18 are located at the lowest point of the hollow partition plates 17, the designed height of the hollow partition plates 17 is higher than the designed height of the leakage holes 18. By tilting both sides of the hollow partition plates 17 and arranging the leakage holes 18 in the middle, the leakage holes 18 can be located at the lowest point of the hollow partition plates 17. As Figure 6 shown, the magnet sheet 20 is located on the right inner side of the hollow partition plates 17 and has a certain height; After the centrifugal force is generated when the discharging component rotates and the centrifugal force overcomes the magnetic force, the discharging component moves outward and exposes the leakage hole 18. When the discharging component stops, since the leakage hole 18 is centered and the two sides of the hollow partition plate 17 are inclined, the blocking plate 19 moves downward obliquely, so that the blocking plate 19 contacts the magnet sheet 20 on the right inner side of the hollow partition plate 17, completing the reset of the blocking plate 19 and blocking the leakage hole 18 again.

[0026] Compared with the traditional valve body structure, although the traditional valve body structure can also achieve the retention of raw materials, there is also the problem that the raw materials do not fall cleanly. Due to the existence of the valve body, in this case, the leakage hole 18 is located at the lowest point of the hollow partition plate 17. Based on the rotation function, most of the raw materials can enter the mixing chamber 1 through the leakage hole 18. For the traditional valve body structure, due to the lack of rotation and the blockage of the valve body, the raw materials passing through are not accurate enough. Therefore, compared with the traditional valve body switch structure, the discharging in this case is more accurate. When the discharging is more accurate, the mixing accuracy is higher and the quality after mixing is better.

[0027] As Figure 4 shown: The dispersing component is arranged based on the stirring shaft 3. The dispersing component includes a frame 21 symmetrically arranged on the outer periphery of the stirring shaft 3 and a number of steel wires 22 evenly arranged in the frame 21. The steel wires 22 are arranged vertically and the gaps between adjacent steel wires 22 are the same.

[0028] Specifically: When the raw materials falling into the mixing chamber 1 through the discharging pipe 10 are impacted by the steel wires 22, the large particles of the raw materials become small particles of the raw materials and then continue to fall. Therefore, the stirring component is arranged based on the stirring shaft 3 and is arranged below the dispersing component. Regarding the stirring component: The stirring component includes a horizontal stirring rod 24 and an inclined stirring rod 23. Both the horizontal stirring rod 24 and the inclined stirring rod 23 are connected to the stirring shaft 3 and the horizontal stirring rod 24 is located below the inclined stirring rod 23. The inclined stirring rods 23 are all inclined upward.

[0029] The reason for setting the horizontal stirring rod 24 and the inclined stirring rod 23 is to ensure multi-angle stirring postures and improve the mixing degree and mixing rate.

[0030] Since the driven gear 13 and the driving gear 9 are provided, in order to ensure the smooth meshing of the above two by preventing the driven gear 13 and the driving gear 9 from being exposed, a protective shell 25 needs to be provided.

[0031] Specifically: A circular groove 5 is formed at the top of the top cover 4, and a magnet is arranged in the circular groove 5. It further includes a protective case 25 that is magnetically connected to the magnet and is used to protect the driving gear 9 and the driven gear 13 and limit the violent shaking of the weighing cylinder 14. The protective case 25 includes a housing, an iron sheet arranged at the bottom of the housing for magnetic connection with the magnet, a protective hole 27 and a motor protection hole 26 formed at the top of the housing. The motor 7 is installed through the motor protection hole 26, and the weighing cylinder 14 is installed through the protective hole 27.

[0032] The quick installation and disassembly of the protective case 25 are completed through the magnet and the iron sheet. The protective case 25 itself can cover the driven gear 13 and the driving gear 9 to avoid the interference of the driven gear 13 and the driving gear 9 by the outside world, thereby ensuring the smooth meshing of the driven gear 13 and the driving gear 9. Since the weighing cylinder 14 will rotate on its own and there is a certain amount of self-rotation vibration, this vibration amount cannot be too large, otherwise it will affect the service life of the weighing cylinder 14. Therefore, a protective hole 27 is provided to alleviate this phenomenon. For the motor protection hole 26, the principle is the same. Because when the motor 7 starts, it will cause vibration of the motor 7. If the vibration amount is too large, it will affect the most basic mixing function of the entire device. Therefore, a motor protection hole 26 is provided to alleviate this phenomenon.

[0033] This device solves the problem that the traditional plant culture solution has mixing errors, which in turn leads to unstable quality of the mixed plant culture solution.

[0034] The above is only a preferred embodiment of the present invention, and it is not intended to limit the present invention in any form. Although the present invention has been disclosed above with the preferred embodiment, it is not intended to limit the present invention. Any person skilled in the art can make some changes or modifications to equivalent embodiments by using the disclosed technical content within the scope of the technical solution of the present invention. However, as long as it does not depart from the content of the technical solution of the present invention, any brief modifications, equivalent changes and modifications made to the above embodiments based on the technical essence of the present invention still fall within the scope of the technical solution of the present invention.

Claims

1. A plant cell culture medium mixing device, characterized in that, It includes a mixing chamber, a top cover detachably connected to the mixing chamber, a motor disposed on the top cover, a stirring shaft coaxially connected to the output shaft of the motor and located inside the mixing chamber, a dispersing assembly connected to the stirring shaft for first dispersing raw materials, and a stirring assembly for subsequent mixing; It further includes a weighing assembly that rotates automatically based on the rotation of the stirring shaft for the raw materials to rotate and fall into the mixing chamber. An outlet assembly is provided on the weighing assembly, and the outlet assembly is opened based on the rotation of the weighing assembly; The weighing assembly includes a number of outlet pipes, auxiliary holes opened on the top cover that match the positions and quantities of the outlet pipes, second rotating bearings installed in the auxiliary holes for connecting the outlet pipes, a connecting pipe detachably connected to one end of the outlet pipe, and a weighing cylinder connected to the upper end of the outlet assembly. A cover plate is detachably connected to the top of the weighing cylinder, and the other end of the connecting pipe is connected to the lower end of the outlet assembly; The connecting pipe is threadedly connected to the outlet pipe. It further includes a driven gear with a mounting hole opened thereon. The connecting pipe passes through the mounting hole and is fixedly connected to the driven gear. The weighing cylinder is transparent and a scale is engraved on the outer wall of the weighing cylinder. The end of the outlet pipe away from the weighing cylinder is located inside the mixing chamber and above the dispersing assembly; The second rotating bearing is installed in the auxiliary hole. A main hole is opened in the center of the top cover, and a first rotating bearing is installed in the main hole. The inner ring of the first rotating bearing is used to connect the outer wall of the stirring shaft. The part of the stirring shaft located outside the mixing chamber is coaxially connected to a driving gear, and the driving gear meshes with the driven gear; The outlet assembly includes a cylinder body, a number of hollow partition plates circumferentially and uniformly fixedly connected inside the cylinder body, leakage holes opened on the hollow partition plates, a plugging plate that can move inside the hollow partition plates, and a magnet sheet provided inside the hollow partition plates for magnetically attracting the plugging plate. When centrifugal force is generated during the rotation of the outlet assembly, the centrifugal force overcomes the magnetic force to change the position of the plugging plate and expose the leakage holes, and the leakage holes are at the lowest point position of the hollow partition plates.

2. The mixing device for a plant cell culture solution according to claim 1, characterized in that, The dispersing assembly is arranged based on the stirring shaft. The dispersing assembly includes a frame symmetrically arranged on the outer periphery of the stirring shaft and a number of uniformly arranged steel wires provided inside the frame. The steel wires are arranged vertically, and the gaps between adjacent steel wires are the same.

3. A plant cell culture medium mixing device according to claim 2, characterized in that, The stirring assembly is arranged based on the stirring shaft and is arranged below the dispersing assembly. The stirring assembly includes horizontal stirring rods and inclined stirring rods. The horizontal stirring rods and the inclined stirring rods are both connected to the stirring shaft, and the horizontal stirring rods are located below the inclined stirring rods. The inclined stirring rods are all inclined upward.

4. A plant cell culture medium mixing device according to claim 3, characterized in that, The mixing chamber and the top cover are made of the same material, both of which are stainless steel. An external thread is provided on the outer top of the mixing chamber, and an internal thread is provided on the inner wall of the top cover. The mixing chamber and the top cover are connected through the external thread and the internal thread.

5. The mixing device for a plant cell culture solution according to claim 4, characterized in that, A circular groove is opened on the top of the top cover, and a magnet is provided in the circular groove. It further includes a protective shell magnetically connected to the magnet for protecting the driving gear and the driven gear and restricting the violent shaking of the weighing cylinder.

6. The mixing device for a plant cell culture solution according to claim 5, characterized in that, The protective shell includes a shell body, an iron sheet provided at the bottom of the shell body for magnetic connection with the magnet, a protective hole and a motor protection hole opened on the top of the shell body. The motor is installed through the motor protection hole, and the weighing cylinder is installed through the protective hole.

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